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JoVE Journal Cancer Research

에 약물 저항 관련 소설 스플 라이스 변종을 감지하는 RNA 시퀀싱을 사용하여

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

Journal (Cancer Research)

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Journal (Cancer Research)

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

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에 약물 저항 관련 소설 스플 라이스 변종을 감지하는 RNA 시퀀싱을 사용하여

Article DOI: 10.3791/54714-v • 09:58 min • December 9th, 2016

December 9th, 2016 •

Rocco Sciarrillo^1,2,3, Anna Wojtuszkiewicz¹, Irsan E. Kooi⁴, Valentina E. Gómez³, Ugo Boggi⁵, Gerrit Jansen⁶, Gert-Jan Kaspers^1,7, Jacqueline Cloos*¹, Elisa Giovannetti*^3,8,9

¹Department of Pediatric Oncology/Hematology, VU University Medical Center, ²Department of Hematology, VU University Medical Center, ³Department of Medical Oncology, VU University Medical Center, ⁴Department of Clinical Genetics, VU University Medical Center, ⁵Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana, Universita’ di Pisa, ⁶Amsterdam Immunology and Rheumatology Center, VU University Medical Center, ⁷Princess Máxima Center for Pediatric Oncology, ⁸Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa, ⁹Institute of Nanoscience and Nanotechnology, CNR-Nano

* These authors contributed equally

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여기에서 우리는 고형 종양 및 혈액 악성 종양에서 약물 내성에 비정상적인 스 플라이 싱의 영향을 조사하기위한 프로토콜을 설명합니다. 이 목표를 위해, 우리는 RNA-서열을 통해 부모와 체외에서 저항 모델의 transcriptomic 프로필을 분석 후보 유전자를 확인하기 위해 QRT-PCR 기반의 방법을 설립했다.

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암 연구 문제 (118) 화학 요법 저항 세포 독성 대체 접합 RNA-SEQ transcriptomics

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