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Journal / Genetics / Halvkvantitativ detektion av RNA-beroende…
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Genetics

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JoVE Journal Genetics
Halvkvantitativ detektion av RNA-beroende RNA-polymeras aktivitet av mänskliga telomeras omvänt transkriptas Protein
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Halvkvantitativ detektion av RNA-beroende RNA-polymeras aktivitet av mänskliga telomeras omvänt transkriptas Protein

Article DOI: 10.3791/57021-v • 08:26 min • June 12th, 2018
June 12th, 2018 •
*1, *1, 1, 1, 1
1Division of Cancer Stem Cell, National Cancer Center Research Institute
* These authors contributed equally

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Mänskliga telomeras omvänt transkriptas (TERT) syntetiserar inte bara telomeric DNA utan också dubbelsträngat RNA genom RNA-beroende RNA-polymeras aktivitet. Här beskriver vi en nyinrättade analys för att upptäcka RNA-beroende RNA-polymeras aktivitet av endogena TERT.

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Genetik fråga 136 TERT RNA-beroende RNA-polymeras dubbelsträngat RNA telomeras immunoprecipitation radioisotoper
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