JoVE Monthly Highlights: March 2017

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Published 3/01/2017
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Chamberlain, N., Kolski-Andreaco, A. JoVE Monthly Highlights: March 2017. J. Vis. Exp. (121), e5832, (2017).

Abstract

Here's a look at what's coming up in the March 2017 issue of JoVE: The Journal of Visualized Experiments.

As the start of spring brings new life, we begin the month investigating exciting new developments in embryo storage for in-vitro fertility treatment. In JoVE Developmental Biology, our authors present a modified technique for vitrification and preservation of human embryos. This aseptic technique uses all FDA-compliant materials including ionomeric resin embryo straws and sterile flexipettes, and allows fast, safe, and repeatable embryo storage independent of operator variation. In addition, with a greater than 95% embryo survival-rate the method contributes to excellent pregnancy outcomes.

Paper cards...printers, it's all so 1990's, right? Well, our next video highlight might make you reconsider. In JoVE Bioengineering this month, our authors reveal 3-dimensional paper microfluidic devices for immunoassays. By patterning paper with hydrophobic barriers, the transfer of fluids can be controlled and directed. Stacking multiple layers of patterned paper creates sophisticated 3-D microfluidic networks that can be utilized in cheap and simple bioanalytical assays, such as the detection of Human Chorionic Gonadotriphin, or hCG - the pregnancy hormone. So don't throw out those old Valentine's Day cards yet - upcycle!

In JoVE Medicine this month, our authors reveal an accurate procedure for quantifying the plaque-forming amyloid-beta protein in cerebral spinal fluid to provide diagnoses in suspected Alzheimer's cases. Current diagnosis is mostly based on mental state and cognitive testing, with some studies beginning to examine amyloid-beta levels, but this is hampered by variability and reproducibility issues. Here, our authors use a combination of solid-phase extraction and liquid chromatography tandem mass spectrometry to quantify amyloid-beta levels to a high degree of accuracy, quantifying the deposition of the protein in plaques in the brain and enhancing the power for accurate diagnoses.

Our final video highlight this month comes from JoVE Behavior, and showcases a new technique to create human body illusions. Here, our authors demonstrate a computer-based virtual hand and face that move in synchrony with the operator's action, creating an illusion that the model is an extension of the participant's own body. This is a useful tool in the study of self-representation, and is an advance over traditional studies requiring demonstrators to mimic actions on participants in time with actions performed on a physical model body part. Ah, just one small step until I realize my dream of becoming a super-fast blue hedgehog.

You've just had a sneak peek of the March 2017 issue of JoVE. Visit the website to see the full-length articles, plus many more, in JoVE: The Journal of Visualized Experiments.

Protocol

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Syrena C. Fernandes, Daniel J. Wilson, Charles R. Mace

Department of Chemistry, Tufts University

We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

Josef Pannee1,2, Kaj Blennow1,2, Henrik Zetterberg1,2,3, Erik Portelius1,2

1Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, Sahlgrenska University Hospital, 2Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 3UCL Institute of Neurology, Queen Square

A reference measurement procedure for the absolute quantification of Aβ1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is described.

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Laura B. Goodman1, Renee R. Anderson1, Marcia Slater2, Elen Ortenberg2, Randall W. Renshaw1, Brittany D. Chilson1, Melissa A. Laverack1, John S. Beeby1, Edward J. Dubovi1, Amy L. Glaser1

1Population Medicine and Diagnostic Sciences, Cornell University Animal Health Diagnostic Center, 2Thermo Fisher Scientific Inc.

High-throughput testing of DNA and RNA based pathogens by nanoscale PCR is described using a syndromic canine and equine respiratory PCR panel.

Creating Virtual-hand and Virtual-face Illusions to Investigate Self-representation

Ke Ma, Dominique P. Lippelt, Bernhard Hommel

Cognitive Psychology Unit, Leiden University

Here, we describe virtual-hand and virtual-face illusion paradigms that can be used to study body-related self-perception/-representation. They have already been used in various studies to demonstrate that, under specific conditions, a virtual hand or face can be incorporated into one's body representation, suggesting that body representations are rather flexible.

Disclosures

No conflicts of interest declared.

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