June 2017: This Month in JoVE

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    Summary

    Date Published: 6/05/2017, Issue 124

    Cite this Article

    Chamberlain, N., Kolski-Andreaco, A. June 2017: This Month in JoVE. J. Vis. Exp. (124), e5841, (2017).

    Abstract

    Here's a look at what's coming up in the June 2017 issue of JoVE: The World's Premier Video Journal

    A "handy" method to test the efficacy of sanitizing techniques is our first feature this month, from JoVE Immunology and Infection. Here, our Authors first "spike" the gloved hands of volunteers with a test organism, and then employ a hand-washing technique of interest. Then, the remaining organisms are collected, and unwashed and test samples are compared. These results allow researchers to provide recommendations for best cleanliness practices during emerging infectious disease outbreaks. We can all hi-five with confidence to that!

    As spring is in full bloom so are allergies, and these are the subjects of our next video highlight. In JoVE Medicine, our Authors describe a novel diagnostic approach for determining specific types of grass pollen allergy using a cohort of patients from Southern China - an area with previously limited data on local allergens. Here, the researchers employed component-resolved diagnostics involving recombinant or purified allergens, to detect sensitization to specific types of grass pollen in test and control individuals. This allows for more targeted treatment of allergic airway diseases in patients with allergic rhinitis or asthma, and that's nothing to sneeze at!

    From grassy gametes to adult plant cells, next we showcase a method for visualizing plant cell wall components, from JoVE Biochemistry. Here, our Authors demonstrate a technique for accurately distinguishing and quantifying cell wall constituents - including those with similar chemical structures such as lignin, cellulose and hemicellulose - using a combination of Raman imaging and multivariate analysis. This method overcomes the barriers to studies of dynamic changes in cell wall components during chemical, physical or biological treatments.

    Finally this month we move from the sessile to the mobile and examine motor neuron development with JoVE Neuroscience. Studying late stage-16 Drosophila embryos, our Author details an immunohistochemistry method to analyze axonal motor neuron projections. By positioning fixed embryos in a filleted arrangement and staining with FasII antibody, the resulting preparations are ideal for the characterization of genes involved in motor axon path-finding and target recognition. It's a neat little technique, with lots of muscle.

    You've just had a sneak peek of the June 2017 issue of JoVE. Visit the website to see the full-length articles, plus many more, in JoVE: The World's Premier Video Journal.

    Protocol

    Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

    Sangyun Jeong

    Department of Molecular Biology, Chonbuk National University

    This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development.

    A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

    Wenting Luo*1,2,3,4, Guichang Pan*5, Huimin Huang1,2,3,4, Peiyan Zheng1,2,3,4, Nili Wei1,2,3,4, Yifei Zhang5, Guangqiao Zeng1,2,3,4, Baoqing Sun1,2,3,4

    1State Key Laboratory of Respiratory Disease, 2National Clinical Research Center of Respiratory Disease, 3Guangzhou Institute of Respiratory Diseases, 4Department of Allergy and Clinical Immunology, First Affiliated Hospital of Guangzhou Medical University, 5Department of Respiratory Medicine, Sixth Affiliated Hospital of Guangzhou Medical University

    This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.

    A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens

    Marlene K. Wolfe, Daniele S. Lantagne

    Department of Civil and Environmental Engineering, Tufts University

    Handwashing is widely recommended to prevent infectious disease transmission. However, there is little evidence on which handwashing methods are most efficacious at removing infectious disease pathogens. We developed a method to assess the efficacy of handwashing methods at removing microorganisms.

    Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall

    Xun Zhang, Sheng Chen, Feng Xu

    Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University

    This protocol aims to present a general method to visualize lignin, cellulose, and hemicellulose in plant cell walls using Raman imaging and multivariate analysis.

    Disclosures

    No conflicts of interest declared.

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