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酵母的4D显微镜
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酵母的4D显微镜

Article DOI: 10.3791/58618-v • 12:00 min • April 28th, 2019
April 28th, 2019 •
1, 1
1Department of Molecular Genetics and Cell Biology, University of Chicago

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该协议描述了使用多色4D(延时3D)共聚焦显微镜对萌芽酵母中荧光标记细胞内腔的分析。选择成像参数是为了捕捉足够的信号,同时限制光损伤。自定义 ImageJ 插件允许跟踪和定量分析标记的结构。

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生物学 问题 146 共聚焦显微镜 4D 成像 荧光 光漂白 去卷积 ImageJ 酵母
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