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McAllister, C., Navani, D. JoVE Monthly Highlights: November 2017. J. Vis. Exp. (129), e5927, (2017).
Here's a look at what's coming up in the November 2017 issue of JoVE: The World's Premier Video Journal.
From a cornucopia of articles for November, we begin with cosmetic applications for chicken feathers. In JoVE Biochemistry, our authors describe a method to prepare keratin hydrolysate from chicken feathers via alkaline-enzymatic hydrolysis, and demonstrate how to test its effect on the skin barrier. These keratin hydrolysate formulations improve skin barrier function by reducing transepidermal water loss.
Without "feather" ado, JoVE Environment goes from the farm to the field. Here, our authors present a methodology to create life tables of Bemisia tabaci, a global agricultural pest. Life tables allow quantification of the rates and sources of mortality in insect populations, and as these methods can be extrapolated to other pests, this research provides a valuable tool for the management of economically expensive pests in agroecosystems.
In JoVE Biology, we switch focus from the pests to the plants, with an experimental procedure for detecting protein-protein interaction dynamics in the tobacco plant Nicotiana benthemiana. A luciferase complementation assay is used to quantify the interaction between two proteins in leaves infiltrated with Agrobacterium tumefaciens. This method provides a simple, rapid proceedure that may be used to answer key questions in the plant signal transduction field.
Our final article, in JoVE Applied Physics, really turns up the heat. Here, our authors describe a series of wind tunnel experiments designed to study the transition of a fire from the ground to canopy in chaparral shrubs. Such experiments may lead to an increased understanding of chaparral crown fires, and can provide knowledge to aid in wildlife prediction and control. For that, we give thanks.
You've just had a sneak peek of the November 2017 issue of JoVE. Visit the website to see the full-length articles, plus many more, in JoVE: The World's Premier Video Journal.
Pavel Mokrejš1, Matouš Huťťa1, Jana Pavlačková2, Pavlína Egner2
The goal of the protocol is to prepare keratin hydrolysate from chicken feathers by alkaline-enzymatic hydrolysis and to test whether adding keratin hydrolysate into a cosmetics ointment base improves skin barrier function (heightening hydration and diminishing transepidermal water loss). Tests are conducted on men and woman volunteers.
Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Kaiwen Sun*, Yuyu Zheng*, Ziqiang Zhu
This manuscript describes an easy and rapid experimental procedure for determining protein-protein interactions based on the measurement of luciferase activity.
Jeanette Cobian-Iñiguez1, AmirHessam Aminfar1, Joey Chong2, Gloria Burke2, Albertina Zuniga1, David R. Weise2, Marko Princevac1
This protocol describes wind tunnel experiments designed to study the transition of a fire from the ground to the canopy of chaparral shrubs.
Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System
Steven E. Naranjo1, Peter C. Ellsworth2
Life tables allow quantification of the sources and rates of mortality in insect populations and contribute to understanding, predicting and manipulating population dynamics in agroecosystems. Methods for conducting and analyzing cohort-based life tables in the field for an insect with sessile immature life stages are presented.
No conflicts of interest declared.