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JoVE Journal
Developmental Biology

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斑马鱼原位杂交染色的基因分型与定量
 
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斑马鱼原位杂交染色的基因分型与定量

Article DOI: 10.3791/59956-v 05:41 min January 28th, 2020
January 28th, 2020

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

基因编辑技术使研究人员能够相对轻松地生成斑马鱼突变体来研究基因功能。在这里,我们提供了一个指南,以执行平行胚胎基因分型和定量的原位杂交信号在斑马鱼。这种无偏的方法在基于原位杂交的表型分析中提供了更高的精度。

Tags

发育生物学,第155期,基因分型,原位杂交,斑马鱼,突变体,偏置,图像定量
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