Journal
/
Neuroscience
/
Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

DOI:
10.3791/60278-v

•

05:52 min

•

October 20, 2019

•

, ,
1CERVO Brain Research Center, Department of Psychiatry and Neurosciences,Université Laval, 2Center for Neural Circuits and Behavior, Department of Neuroscience and Section of Neurobiology, Division of Biology,University of California at San Diego, 3Neurophotometrics Ltd.

Chapters

  • 00:04Title
  • 00:49Optical Path Between CMOS and Patch Cord
  • 01:58Setup of ROIs and Recording Arena
  • 03:09In-vivo and Dual-color Recordings
  • 04:52Results: Florescence Increased After Administration of Air Puffs
  • 05:18Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol details how to implement and perform multi-fiber photometry recordings, how to correct for calcium-independent artifacts, and important considerations for dual-color photometry imaging.

Tags

Multi-fiber PhotometryFiber PhotometryCSOM ImagingNeural ActivityFreely-moving AnimalsGenetically Encoded Custom IndicatorOptical FibersFive-axis TranslatorCMOS CameraRegions Of Interest (ROIs)Fiber LabelingRecording Arena Setup

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

Recording Single Neurons’ Action Potentials from Freely Moving Pigeons Across Three Stages of Learning

Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array

A Novel Behavioral Assay to Investigate Gustatory Responses of Individual, Freely-moving Bumble Bees (Bombus terrestris)

Recording EEG in Freely Moving Neonatal Rats Using a Novel Method

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

Noninvasive EEG Recordings from Freely Moving Piglets

Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

Read Article