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JoVE Journal Bioengineering
Dextran Merking og opptak i Live og funksjonell Murine Cochlear Hair Cells
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Dextran Merking og opptak i Live og funksjonell Murine Cochlear Hair Cells

Article DOI: 10.3791/60769-v • 05:55 min • February 8th, 2020
February 8th, 2020 •
1, 1
1Molecular Physiology and Biophysics Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health

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Her presenterer vi en metode for å visualisere opptaket av 3 kDa Texas Red-merket dextran i auditive hårceller med funksjonelle mechanotransduction kanaler. I tillegg kan dextrans på 3-10 kDa brukes til å studere endocytose i håret og støtte celler i orgelet Corti.

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Bioengineering Utgave 156 Dextran indre øre hårceller mechanotransduction kanal fargeopptak endocytose intracellulære vesikler konfokal mikroskopi
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