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JoVE Journal Developmental Biology
A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
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A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals

Article DOI: 10.3791/60809-v • 08:06 min • February 14th, 2020
February 14th, 2020 •
1, 1, 1
1Cell, Developmental and Integrative Biology Department, University of Alabama at Birmingham

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Summary
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We describe the isolation, dispersion and plating of dental pulp (DP) primary cells with trigeminal (TG) neurons cultured atop overlying transwell filters. Cellular responses of DP cells can be analyzed with immunofluorescence or RNA/protein analysis. Immunofluorescence of neuronal markers with confocal microscopy permits the analysis of neurite outgrowth responses.

Tags

Co-culture Method Neurite Outgrowth Dental Pulp Paracrine Signals Dental Pulp Stem Cells Trigeminal Neurons Crosstalk Cell Populations Outcomes Measurement Neural Tissue Repair Traumatic Events Neurodegenerative Diseases Protocol Stages Dispersion Optimization Replicates Euthanization Mouse Mandible Separation Maxilla Removal Tongue Removal Dissecting Microscope Alveolar Bone Tissue
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