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Isolation og karakterisering af voksne hjertefibroblaster og Myofibroblaster
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Isolation og karakterisering af voksne hjertefibroblaster og Myofibroblaster

Article DOI: 10.3791/60909-v • 10:45 min • March 12th, 2020
March 12th, 2020 •
1, 1, 1,2, 1
1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 2American Red Cross, National Headquarters

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Opnåelse af en ren population af fibroblaster er afgørende for at studere deres rolle i sårreparation og fibrose. Beskrevet her er en detaljeret metode til at isolere fibroblaster og myofibroblaster fra uskadte og tilskadekomne musehjerter efterfulgt af karakterisering af deres renhed og funktionalitet ved immunfluorescens, RTPCR, fluorescens-assisteret cellesortering, og kollagen gel sammentrækning.

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Biologi isolation αSMA fluorescens aktiveret cellesortering fibroblaster myofibroblast kollagengel immunfluorescens MEFSK4
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