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Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination
JoVE Journal
Biology
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JoVE Journal Biology
Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination
DOI:

06:18 min

June 03, 2020

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Chapters

  • 00:04Introduction
  • 00:36Laboratory Bench and Tool Preparation
  • 01:13Sub-Sampling and Sample Preparation
  • 01:59Plate Preparation
  • 02:01Plate Preparation
  • 02:29Sub-Sample Transfer
  • 05:07Results: Representative Blank Well DNA Concentration
  • 05:50Special Consideration

Summary

Automatic Translation

Current methods for loading 96-well plates for DNA extractions can be prone to contamination. We detail a new method for loading 96-well plates that reduces risk of cross-contamination among wells. Our method will help other researchers capitalize on the efficiency of high-throughput DNA extraction techniques and minimize risk of contamination.

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