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Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination

Article DOI: 10.3791/61405-v • 06:18 min • June 3rd, 2020
June 3rd, 2020 •
*1,2, *3,4
1Department of Ecosystem Science and Management, University of Wyoming, 2Program in Ecology, University of Wyoming, 3EPSCoR-IDEA, University of Wyoming, 4Department of Botany, University of Wyoming
* These authors contributed equally

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Current methods for loading 96-well plates for DNA extractions can be prone to contamination. We detail a new method for loading 96-well plates that reduces risk of cross-contamination among wells. Our method will help other researchers capitalize on the efficiency of high-throughput DNA extraction techniques and minimize risk of contamination.

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High-throughput DNA Extraction Plates Contamination Reduction Loading Efficiency Sample Cross-contamination Methodology Microbiome Research Benchtop Sterilization Tools Sterilization Sub-sampling Sample ID Assignment Centrifuge Tubes Extraction Blank PCR Tubes 96-well Plate Preparation
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