ניטור אקטין פירוק עם מיקרוסקופיה זמן לשגות

Published 11/08/2006
1 Comment
  CITE THIS  SHARE 
Biology

Your institution must subscribe to JoVE's Biology section to access this content.

Fill out the form below to receive a free trial or learn more about access:

Welcome!

Enter your email below to get your free 10 minute trial to JoVE!





By clicking "Submit", you agree to our policies.

 

Summary

Cite this Article

Copy Citation

Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).

Please note that all translations are automatically generated.

Click here for the english version. For other languages click here.

Abstract

Protocol

  1. לבנות תא תזרים כפי שמוצג בסרטון. ודא את החותם parafilm הדוקה ולהשתמש coverslips שטף.
  2. דגירה אקטין מחייב סוכן בחדר עבור '5-10.
  3. בלוק הלא ספציפית אתרי הקישור על coverslip הזכוכית עם חלבון חסימה.
  4. אקטין לפלמר בתוך החדר על ידי זורם G-אקטין ב polymerizing חיץ.
  5. כשלון unpolymerized אקטין ידי זורם חיץ עודף.

Subscription Required. Please recommend JoVE to your librarian.

Comments

1 Comment

  1. What is the second pair of shorter parafilm strips for? Why do you polymerize in the chamber and how? G-actin in polymerization buffer. Is't that F-actin? What is the actin binding agent and blocking protein and their buffers and their concentrations?

    Reply
    Posted by: Anonymous
    July 28, 2009 - 2:41 PM

Post a Question / Comment / Request

You must be signed in to post a comment. Please or create an account.

Video Stats