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Patch di registrazione pinza dei canali ionici Espressa in ovociti di Xenopus
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Patch di registrazione pinza dei canali ionici Espressa in ovociti di Xenopus

Article DOI: 10.3791/936-v • 08:01 min • October 16th, 2008
October 16th, 2008 •
1, 2, 2
1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Molecular and Cellular Physiology, Stanford University School of Medicine

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Questo è inteso come una introduzione alla registrazione di patch clamp da ovociti di Xenopus laevis. Esso copre la rimozione della membrana vitellina, la formazione di un sigillo gigaohm (gigaseal), e la conversione facoltativa delle patch al di fuori-out topologia.

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Biologia Cellulare Numero 20 Elettrofisiologia patch clamp pinza di tensione ovociti Biofisica Gigaseal canali ionici
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