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Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, An
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 10-30-2014
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A Gram-staining-negative, strictly aerobic, yellowish-orange-pigmented, flexirubin-positive, rod-shaped, non-flagellated, non-spore-forming and non-gliding marine bacterium, designated strain CC-PY-50T, was isolated from estuarine water off Pingtung, Taiwan. Strain produced zeaxanthin as a major carotenoid pigment, and showed highest pairwise 16S rRNA gene sequence similarity to Bizionia hallyeonensis T-y7T (93.9 %) followed by Corallibacter vietnamensis KMM 6217T (93.8 %), Geojedonia litorea YCS-16T (93.7 %) and other Flavobacteriaceae members (<93.7 %). Strain CC-PY-50T established a distinct phyletic lineage associated with Mangrovimonas yunxiaonensis LYYY01T (93.1 % sequence similarity) with poor bootstrap support during neighbor-joining and maximum-likelihood phylogenetic analyses (37 % for each). The polar lipid profile of strain CC-PY-50T characterized to accommodate large number of unknown lipids including major amounts of three unidentified aminolipids and two unidentified lipids, and moderate amounts of an unidentified phospholipid, an unidentified glycolipid and an unidentified lipid. In addition, phosphatidylethanolamine was also detected in significant amounts. The major (>5 % of total) fatty acids were iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH, C16:0 and C16:1 ?6c and/or C16:1 ?7c. The DNA G + C content was 37.1 mol% and menaquinone-6 (MK-6) was the sole respiratory quinone. Based on the phylogenetic evidence and several distinguishing phenotypic and chemotaxonomic features, strain CC-PY-50T is proposed to represent a novel genus and species of the family Flavobacteriaceae, for which the name Hanstruepera neustonica gen. nov., sp. nov. is proposed. The type strain of the type species Hanstruepera neustonica is CC-PY-50T (=JCM 19743T =BCRC 80747T). The emended descriptions of the species Sediminibacter furfurosus, Mangrovimonas yunxiaonensis, Antarcticimonas flava and Hoppeia youngheungensis are also proposed.
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Cribrihabitans neustonicus sp. nov., isolated from coastal surface seawater, and emended description of the genus Cribrihabitans Chen et al. 2014.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 09-01-2014
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A Gram-stain-negative, strictly aerobic, rod- or oval-shaped, motile, non-spore-forming bacterium, designated strain CC-AMHB-3(T), was isolated from coastal surface seawater off Hualien, Taiwan. The novel strain showed high pairwise 16S rRNA gene sequence similarities to Ruegeria mobilis NBRC 101030(T) (96.5?%), Ruegeria scottomollicae LMG 24367(T) (96.4?%), Phaeobacter aquaemixtae SSK6-1(T) (96.2?%), Phaeobacter daeponensis TF-218(T) (96.2?%), Cribrihabitans marinus CZ-AM5(T) (96.1?%) and other species of the family Rhodobacteraceae (?95.9?%). However, strain CC-AMHB-3(T) formed a distinct phyletic lineage associated with C. marinus CZ-AM5(T) during phylogenetic analyses. The polar lipid profile of strain CC-AMHB-3(T) included major amounts of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine; moderate amounts of phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid; and trace amounts of an unidentified lipid and an unidentified phospholipid, which was qualitatively almost in line with that of C. marinus CZ-AM5(T) but remarkably distinct as compared with the type species of the genera Ruegeria (Ruegeria atlantica JCM 21234(T)) and Phaeobacter (Phaeobacter gallaeciensis JCM 21319(T)). In line with the fatty acid profile of C. marinus CZ-AM5(T), the major (>5?% of total) fatty acids of strain CC-AMHB-3(T) were C18?:?1?7c and/or C18?:?1?6c (summed feature 8), 11-methyl C18?:?1?7c and C16?:?0. The DNA G+C content was 66.7 mol%. Ubiquinone-10 (Q-10) was the sole respiratory quinone. Thus, based on the results of the polyphasic study presented here, strain CC-AMHB-3(T) is considered to represent a novel species of the genus Cribrihabitans, for which the name Cribrihabitans neustonicus sp. nov. is proposed; the type strain is CC-AMHB-3(T) (?=?JCM 19537(T)?=?BCRC 80695(T)). In addition, an emended description of the genus Cribrihabitans is also proposed.
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Description of Algoriphagus taiwanensis sp. nov., a xylanolytic bacterium isolated from surface seawater, and emended descriptions of Algoriphagus mannitolivorans, Algoriphagus olei, Algoriphagus aquatilis and Algoriphagus ratkowskyi.
Antonie Van Leeuwenhoek
PUBLISHED: 06-17-2014
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A novel, Gram-stain negative, strictly aerobic, oval or rod-shaped, red-pigmented, non-spore-forming xylanolytic bacterial strain, designated CC-PR-82(T), was isolated from surface seawater of Pingtung coast, Taiwan, and characterized by polyphasic taxonomy. Strain CC-PR-82(T) shared highest pairwise 16S rRNA gene sequence similarity to Algoriphagus mannitolivorans IMSNU 14012(T) (97.0 %) followed by 'A. boseongensis' BS-R1(T) (96.5 %) and A. olei CC-Hsuan-617(T) (95.6 %), whereas other (n = 26) Algoriphapus species shared 95.6-92.6 % sequence similarities. The novel strain further established a distinct phyletic lineage tightly associated with Algoriphagus species. The DNA-DNA hybridization value obtained between CC-PR-82(T) and A. mannitolivorans DSM 15301(T) was 29.0 % (33.4 % reciprocal using A. mannitolivorans DSM 15301(T) probe). The major (>5 % of total) fatty acids were identified as iso-C15:0, C16:1 ?6c and/or C16:1 ?7c, iso-C16:0, iso-C17:1 ?9c and/or C16:0 10-methyl, anteiso-C15:0 and C17:1 ?6c. Polar lipids included major amounts of an unidentified aminolipid and an unidentified lipid; moderate amounts of phosphatidylethanolamine, phosphatidylserine and an unidentified aminolipid; trace amounts of an unidentified phospholipid, an unidentified lipid and an unidentified glycolipid. The DNA G+C content was determined to be 42.3 mol%. Menaquinone-7 was the sole respiratory quinone. Based on the polyphasic characteristics, that are in line with those of Algoriphagus species, in addition to distinguishing phylogenetic and phenotypic features, strain CC-PR-82(T) appears to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus taiwanensis sp. nov. (type strain CC-PR-82(T) = JCM 19755(T) = BCRC 80746(T)) is proposed. In addition, emended descriptions of the species A. mannitolivorans, A. aquatilis, A. olei and A. ratkowskyi are also proposed.
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Rhizobium straminoryzae sp. nov., isolated from the surface of rice straw.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 06-04-2014
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An aerobic, Gram-stain-negative, rod-shaped bacterium, designated strain CC-LY845(T), was isolated from the surface of rice straw in Taiwan. Cells were non-motile, and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus Rhizobium, with closest similarity to Rhizobium pseudoryzae KCTC 23294(T) (97.6?%), R. rhizoryzae KCTC 23652(T) (97.0?%) and R. oryzae LMG 24253(T) (96.7?%); other species showed lower levels of similarity (<96.6?%). The DNA-DNA relatedness of strain CC-LY845(T) and R. pseudoryzae KCTC 23294(T) was 34.8±3.1?% (reciprocal value 39.2±2.2?%). Phylogenetic analysis based on the housekeeping atpD and recA genes showed that the novel strain could be distinguished from R. pseudoryzae KCTC 23294(T) (92.7 and 91.5?%, respectively) and other species of the genus Rhizobium. The temperature range for growth was 25-42 °C, the pH range was 5.0-9.0 and NaCl concentrations up to 4.0?% (w/v) were tolerated. Strain CC-LY845(T) did not form nodules on four different legumes, and the nodD and nifH genes were not detected by PCR. The major fatty acids were C16?:?0 and summed feature 8 (C18?:?1?7c/C18?:?1?6c). The polyamine pattern of strain CC-LY845(T) showed spermidine and putrescine as major polyamines. The predominant quinone system was ubiquinone 10 (Q-10). The DNA G+C content was 68.3±2.4 mol%. Base on its phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY845(T) is proposed to represent a novel species within the genus Rhizobium, for which the name Rhizobium straminoryzae sp. nov. is proposed. The type strain is strain CC-LY845(T) (?=?BCRC 80698(T)?=?JCM 19536(T)).
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Youngimonas vesicularis gen. nov., sp. nov., of the family Rhodobacteraceae, isolated from surface seawater, reclassification of Donghicola xiamenensis Tan et al. 2009 as Pseudodonghicola xiamenensis gen. nov., comb. nov. and emended description of the genus Donghicola
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 05-20-2014
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A Gram-staining-negative, non-pigmented, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, devoid of bacteriochlorophyll, designated strain CC-AMW-E(T), was isolated from surface seawater off the coast at Kending, Taiwan. Strain CC-AMW-E(T) shared 95.7 and 93.9% 16S rRNA gene sequence similarity, respectively, with the type strains of the type species of the genera Donghicola (Donghicola eburneus SW-277(T)) and Roseovarius (Roseovarius tolerans EL-172(T)). The predominant (>75% of the total) fatty acid was summed feature 8 (C(18?:?1)?6c and/or C(18?:?1)?7c). The polar lipid profile included major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and an unidentified aminolipid. In addition, moderate amounts of an unidentified lipid and trace amounts of an unidentified phospholipid were detected. The DNA G+C content was 67.9 mol%. Ubiquinone Q-10 was the sole respiratory quinone. Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics (in particular its polar lipid pattern), we conclude that strain CC-AMW-E(T) represents a novel genus and species of the family Rhodobacteraceae, for which the name Youngimonas vesicularis gen. nov., sp. nov. is proposed. The type strain of Youngimonas vesicularis is CC-AMW-E(T) (?=?JCM 18819(T)?=?BCRC 80549(T)). In addition, an emended description of the genus Donghicola Yoon et al. 2007 and the reclassification of Donghicola xiamenensis Tan et al. 2009 as Pseudodonghicola xiamenensis gen. nov., comb. nov. (type strain Y-2(T)?=?MCCC 1A00107(T)?=?LMG 24574(T)?=?CGMCC 1.7081(T)) are proposed.
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Gramella oceani sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from marine sediment.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 05-16-2014
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A Gram-staining-negative, yellow-pigmented, strictly aerobic, zeaxanthin-producing, rod-shaped, non-endospore-forming, appendaged bacterial strain that exhibits gliding motility, designated CC-AMSZ-T(T), was isolated from marine sediment off coastal Kending, Taiwan. Strain CC-AMSZ-T(T) shared 94.9% and 96.7-94.1% 16S rRNA gene sequence similarities with Gramella echinicola KMM 6050(T) and other species of the genus Gramella, respectively, and formed a distinct phyletic lineage in phylogenetic trees. The major (?5% of the total) fatty acids were C(16?:?0), iso-C(15?:?0), anteiso-C(15?:?0), C(16?:?1)?6c and/or C(16?:?1)?7c and iso-C(17?:?1)?9c and/or C(16?:?0) 10-methyl. Phosphatidylethanolamine, six unidentified lipids and three unidentified aminolipids were the polar lipid components. The DNA G+C content was 38.6 mol%. The predominant respiratory quinone was menaquinone-6 (MK-6). Based on the phylogenetic distinctiveness and distinguishing phenotypic characteristics, strain CC-AMSZ-T(T) represents a novel species of the genus Gramella, for which the name Gramella oceani sp. nov. is proposed. The type strain is CC-AMSZ-T(T) (?=?JCM 18809(T)?=?BCRC 80547(T)).
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Pseudomonas hussainii sp. nov., isolated from droppings of a seashore bird, and emended descriptions of Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 04-17-2014
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Two Gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strains that are motile by a monopolar flagellum, designated CC-AMH-11(T) and CC-AMHZ-5, were isolated from droppings of a seashore bird off the coast of Hualien, Taiwan. The strains showed 99.7% mutual pairwise 16S rRNA gene sequence similarity, while exhibiting <96.2% sequence similarity to strains of other species of the genus Pseudomonas (95.7-95.9% similarity with type species, Pseudomonas aeruginosa LMG 1242T), and formed a distinct co-phyletic lineage in the phylogenetic trees. The common major fatty acids (>5% of the total) were C18 : 1?7c and/or C18 : 1?6c (summed feature 8), C16 : 1?6c and/or C16 : 1?7c (summed feature 3), C16 : 0 and C12 : 0. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine, an unidentified lipid and an unidentified phospholipid were detected as common polar lipids. The DNA G+C contents of strains CC-AMH-11(T) and CC-AMHZ-5 were 61.1 and 61.6 mol%, respectively. The common major respiratory quinone was ubiquinone 9 (Q-9), and the predominant polyamine was putrescine. The DNA-DNA hybridization obtained between the two strains was 79.0% (reciprocal value 89.4% using CC-AMHZ-5 DNA as the probe). The very high 16S rRNA gene sequence similarity and DNA-DNA relatedness and the poorly distinguishable phenotypic features witnessed between CC-AMH-11(T) and CC-AMHZ-5 suggested unambiguously that they are two distinct strains of a single genomic species. However, the strains also showed several genotypic and phenotypic characteristics that distinguished them from other closely related species of Pseudomonas. Thus, the strains are proposed to represent a novel species of Pseudomonas, for which the name Pseudomonas hussainii sp. nov. is proposed. The type strain is CC-AMH-11(T) (?= JCM 19513(T)?= BCRC 80696(T)); a second strain of the same species is CC-AMHZ-5 (?= JCM 19512 = BCRC 80697). In addition, emended descriptions of the species Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis are also proposed.
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Description of Niveispirillum fermenti gen. nov., sp. nov., isolated from a fermentor in Taiwan, transfer of Azospirillum irakense (1989) as Niveispirillum irakense comb. nov., and reclassification of Azospirillum amazonense (1983) as Nitrospirillum amazonense gen. nov.
Antonie Van Leeuwenhoek
PUBLISHED: 04-15-2014
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A taxonomic study was carried out on a novel aerobic bacterial strain (designated CC-LY736(T)) isolated from a fermentor in Taiwan. Cells of strain CC-LY736(T) were Gram-stain negative, spiral-shaped and motile by means of a monopolar flagellum. Strain CC-LY736(T) shared the greatest degree of 16S rRNA gene sequence similarity to Azospirillum irakense DSM 11586(T) (97.2 %), Rhodocista centenaria JCM 21060(T) (96.3 %) and Rhodocista pekingensis JCM 11669(T) (96.1 %). The major fatty acids were C16:0, C16:1 ?5c, C19:0 cyclo ?8c, C18:1 ?7c/C18:1 ?6c, C16:0 3-OH and C18:1 2-OH. The predominant polar lipids included phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidyldimethylethanolamine and two unidentified glycolipids. The common major respiratory quinone was ubiquinone Q-10 and predominant polyamines were sym-homospermidine and putrescine. The DNA G+C content of strain CC-LY736(T) was 67.6 ± 0.1 mol %. During phylogenetic analysis, strain CC-LY736(T) formed a unique phyletic lineage associated with Rhodocista species. However, the combination of genetic, chemotaxonomic and physiological data clearly indicated that strain CC-LY736(T) was a novel representative of the family Rhodospirillaceae. Based on the polyphasic comparison, the name Niveispirillum fermenti gen. nov., sp. nov. is proposed; the type strain of the type species is CC-LY736(T) (= BCRC 80504(T) = LMG 27263(T)). In addition, the reclassifications of Azospirillum irakense as Niveispirillum irakense comb. nov. (type strain KBC1(T) = ATCC 51182(T) = BCRC 15764(T) = CIP 103311(T)), and Azospirillum amazonense as Nitrospirillum amazonense gen. nov., sp. nov. (type strain Am14(T) = ATCC 35119(T) = BCRC 14279(T) = DSM 3787(T)) are proposed based on the polyphasic taxonomic data obtained in this study.
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Gramella planctonica sp. nov., a zeaxanthin-producing bacterium isolated from surface seawater, and emended descriptions of Gramella aestuarii and Gramella echinicola.
Antonie Van Leeuwenhoek
PUBLISHED: 01-30-2014
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A Gram-stain negative, strictly aerobic, zeaxanthin-producing, rod-shaped, non-spore-forming bacterial strain which is motile by gliding, designated CC-AMWZ-3(T), was isolated from surface seawater off coastal Kending, Taiwan. Strain CC-AMWZ-3(T) was found to share 93.3 % and 96.0-92.4 % pairwise 16S rRNA gene sequence similarity to Gramella echinicola KMM 6050(T) and other Gramella species, respectively, and formed distinct phyletic lineage during phylogenetic analysis. The major fatty acids were identified as C16:0, iso-C15:0, anteiso-C15:0, C16:1 ?6c and/or C16:1 ?7c and iso-C17:1 ?9c and/or C16:0 10-methyl. Polar lipids were found to include phosphatidylethanolamine, six unidentified lipids and three unidentified aminolipids. The DNA G+C content was determined to be 40.6 mol%. Menaquinone-6 was the sole respiratory quinone identified and triamine-sym-homospermidine was the predominant polyamine. Based on the polyphasic characteristics that are in line with those of Gramella species, in addition to distinguishing phylogenetic and phenotypic features, strain CC-AMWZ-3(T) appears to represent a novel species of the genus Gramella, for which the name Gramella planctonica sp. nov. (type strain CC-AMWZ-3(T) = JCM 18807(T) = BCRC 80553(T)) is proposed. In addition, emended descriptions of the species Gramella aestuarii and Gramella echinicola are also proposed.
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Novosphingobium arabidopsis sp. nov., a novel DDT-resistant bacterium isolated from the Arabidopsis thaliana rhizosphere.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 10-24-2013
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An aerobic, Gram-stained-negative, rod-shaped, DDT-resistant bacterium (defined strain CC-ALB-2T) was isolated from the Arabidopsis thaliana rhizosphere. Strain CC-ALB-2T is able to grow at 25-37°C, pH 5.0-8.0, NaCl 1.0% (w/v) and tolerate DDT up to 200 mg l-1. The 16S rRNA gene sequence analysis of strain CC-ALB-2T showed highest sequence similarity to Novosphingobium stygium KCTC 2891T (97.1%) and Novosphingobium soli DSM 22821T (96.8%), and showed lower levels of similarity (<97.0%) to other Novosphingobium species. The major fatty acid profile consists of C14:0 2OH (13.1%), C16:0 (10.0%), C15:0 iso 3OH (5.8%), C16:1 ?7c / C16:1 ?6c (24.7%) and C18:1 ?7c / C18:1 ?6c (42.4%). The polar lipid profile constitutes sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylglycerol and phosphatidyl choline. The polyamine pattern showed predominance of spermidine as major polyamine. The predominant quinone system is ubiquinone (Q-10). The DNA G+C content is 68.9±0.1 mol%. Base on the phylogenetic, phenotypic and chemotaxonomic features, strain CC-ALB-2T is proposed to represent a novel species within the genus Novosphingobium for which the name Novosphingobium arabidopsis sp. nov. (=BCRC 80571T =JCM 18896T) is proposed.
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Robertkochia marina gen. nov., sp. nov., of the family Flavobacteriaceae isolated from surface seawater, and emended descriptions of the genera Joostella and Galbibacter.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 10-14-2013
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A Gram-staining-negative, orange-pigmented, strictly aerobic, carotenoid-producing, rod-shaped, non-flagellated, non-spore-forming bacterium motile by gliding designated strain CC-AMO-30DT was isolated from surface seawater collected near Taichung harbor, Taiwan. Strain CC-AMO-30DT shared pairwise 16S rRNA gene sequence similarities of 94.8, 93.7 and 92.5 % to the type species of the genera Joostella, Pustulibacterium and Galbibacter, respectively, and formed a distinct monophyletic lineage in phylogenetic trees. The major fatty acids (? 5 % of total fatty acids) were iso-C 15:1 G, iso-C15:0, iso-C17:0 3-OH, iso-C15:0 3-OH, iso-C16:0 and anteiso-C15:0. Polar lipid profile consisted of phosphatidylethanolamine, four unidentified lipids, two unidentified aminolipids and an unidentified phospholipid. The major polyamine was triamine sym-homospermidine. The DNA G + C content was 47.1 mol% and the predominant respiratory quinone was menaquinone-6 (MK-6). Based on the phylogenetic distinctiveness, and distinguishing phenotypic and chemotaxonomic characteristics, strain CC-AMO-30DT represents a novel genus and species of the family Flavobacteriaceae, for which the name Robertkochia marina gen. nov., sp. nov. is proposed; the type strain of the type species is CC-AMO-30DT (= JCM 18552T = BCRC 80469T). Emended descriptions of the genera Joostella and Galbibacter are also proposed.
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Chitinophaga taiwanensis sp. nov., a novel bacterium isolated from the Arabidopsis thaliana rhizosphere.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 10-04-2013
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An aerobic, Gram-negative, rod-shaped bacterium (strain CC-ALB-1T) was isolated from the Arabidopsis thaliana rhizosphere. Strain CC-ALB-1T is able to grow at 20-30 °C, pH 5.0-8.0 and NaCl 1.0% (w/v). The 16S rRNA gene sequence analysis of strain CC-ALB-1T showed highest sequence similarity to Chitinophaga ginsengisegetis KCTC 12654T (96.9%) and Chitinophaga niastensis JCM 15441T (96.7%), and other species showed lower levels of similarity (<97.0%) to all other Chitinophaga species. The fatty acid profile consists of C15:0 iso, C17:0 iso 3OH, C15:1 ?5c, C16:1 ?5c and C16:1 ?7c/C16:1 ?6c. The polar lipid profile constitutes phosphatidylethanolamine (PE), unidentified aminolipids (AL1-2) and unidentified lipids (L1-5). The predominant quinone system is menaquinone (MK-7). The DNA G+C content is 53.4±0.4 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic features, strain CC-ALB-1T is proposed to represent a novel species within the genus Chitinophaga for which the name Chitinophaga taiwanensis sp. nov. (=BCRC 80570T =JCM 18895T) is proposed.
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Aquibacter zeaxanthinifaciens gen. nov., sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from surface seawater, and emended descriptions of the genera Aestuariibaculum and Gaetbulibacter.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 09-18-2013
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A Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming and gliding marine bacterium designated strain CC-AMZ-304T was isolated from coastal surface seawater near Taichung harbor, Taiwan. Strain CC-AMZ-304T predominantly synthesized zeaxanthin and thus formed yellow-colored colonies on marine agar. The novel strain showed unstable phylogenetic position, although sharing high pairwise 16S rRNA gene sequence similarities of 95.9-94.9, 95.7 and 95.1-93.9 % with Gaetbulibacter species (n=4), Aestuariibaculum suncheonense SC17T and Bizionia species (n=7), respectively. The polar lipid profile of strain CC-AMZ-304T consisted of phosphatidylethanolamine, five unidentified lipids, one unidentified phospholipid, two unidentified aminolipids and one unidentified glycolipid. The major (> 5 % of the total) fatty acids were C15:0 iso, C15:1 iso G, C17:0 iso 3-OH, C15:0 iso 3-OH and C 15:1 ?5c. The DNA G + C content was 36.0 mol%. Menaquinone-6 (MK-6) was the sole respiratory quinone and the major polyamine was triamine sym-homospermidine. Phylogenetic distinctiveness, unique polar lipid composition, presence of significant amounts of branched hydroxyl fatty acids (C17:0 iso 3-OH and C15:0 iso 3-OH ) and of low amount of C15:0 anteiso, and several additional distinguishing biochemical features clearly discriminated strain CC-AMZ-304T from the type species of the genera Aestuariibaculum and Gaetbulibacter. Thus, based on the present polyphasic evidences, strain CC-AMZ-304T is proposed to represent a novel genus and species within the family Flavobacteriaceae, for which the name Aquibacter zeaxanthinifaciens gen. nov., sp. nov. proposed; the type strain is of the type species is CC-AMZ-304T (= JCM 18557T = BCRC 80463T). Emended descriptions of the genera Aestuariibaculum and Gaetbulibacter are also proposed.
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Kordia aquimaris sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from surface seawater, and emended description of the genus Kordia.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 08-30-2013
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A Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming and gliding marine bacterium designated strain CC-AMZ-301(T) was isolated from coastal surface seawater near Taichung harbour, Taiwan. Strain CC-AMZ-301(T) predominantly synthesized zeaxanthin and thus formed yellow colonies on marine agar. The novel strain showed high pairwise 16S rRNA gene sequence similarity to Kordia periserrulae IMCC1412(T) (95.0?%), K. algicida KCTC 8814P(T) (94.4?%) and K. antarctica IMCC3317(T) (94.2?%), and formed a phylogenetic lineage tightly associated with species of the genus Kordia. The polar lipid profile of strain CC-AMZ-301(T) consisted of three unidentified lipids, three unidentified aminolipids and one unidentified phospholipid. The major fatty acids (>5?% of total) were iso-C17?:?0 3-OH, iso-C15?:?0, summed feature 3 (C16?:?1?7c and/or C16?:?1?6c) and summed feature 9 (iso-C17?:?1?9c and/or 10-methyl C16?:?0). The DNA G+C content was 36.4 mol%. Menaquinone 6 (MK-6) was the predominant respiratory quinone. The major polyamine was the triamine sym-homospermidine. Based on evidence from the present polyphasic study, strain CC-AMZ-301(T) is proposed to represent a novel species of the genus Kordia, for which the name Kordia aquimaris sp. nov. is proposed; the type strain is CC-AMZ-301(T) (?=?JCM 18556(T)?=?BCRC 80464(T)). An emended description of the genus Kordia is also proposed.
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Luteibaculum oceani gen. nov., sp. nov., a carotenoid-producing, lipolytic bacterium isolated from surface seawater, and emended description of the genus Owenweeksia Lau et al. 2005.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 08-29-2013
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A yellow-pigmented, Gram-staining-negative, strictly aerobic, rod-shaped, non-flagellated, non-spore-forming, lipolytic and gliding marine bacterium designated strain CC-AMWY-103B(T) was isolated from surface seawater collected at Kending, Taiwan. The strain shared the highest 16S rRNA gene sequence similarity of 89.4?% with Owenweeksia hongkongensis JCM 12287(T) and Brumimicrobium mesophilum YH207(T), and established a distinct phyletic lineage associated with the members of the family Cryomorphaceae. The polar lipid profile of strain CC-AMWY-103B(T) consisted of phosphatidylethanolamine, ten unidentified lipids and four unidentified aminolipids. The major fatty acids (>5?% of the total) were iso-C15?:?0, iso-C15?:?1 G, C15?:?1?5c, iso-C17?:?0 3-OH and C15?:?1?8c. The DNA G+C content was 44.2±0.3 mol%. The predominant respiratory quinone was menaquinone-6 (MK-6) and the major polyamine was spermidine. Based on its genetic, phylogenetic, phenotypic and chemotaxonomic distinctiveness, strain CC-AMWY-103B(T) is proposed to represent a distinct member of the family Cryomorphaceae, for which the name Luteibaculum oceani gen. nov., sp. nov. is proposed; the type strain of Luteibaculum oceani is CC-AMWY-103B(T) (?=?JCM 18817(T)?=?BCRC 80551(T)). An emended description of the genus Owenweeksia is also proposed.
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Pseudomonas guguanensis sp. nov., a gammaproteobacterium isolated from a hot spring.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 08-05-2013
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An aerobic, Gram-stain-negative, rod-shaped bacterium (designated strain CC-G9A(T)), motile by a polar-flagellum, was isolated from a hot spring water sample in Taiwan. Strain CC-G9A(T) could grow at 20-42 °C, pH 6.0-10.0 and tolerate up to 7?% (w/v) NaCl. The 16S rRNA gene sequence analysis of strain CC-G9A(T) showed pairwise sequence similarity to Pseudomonas mendocina LMG 1223(T) (97.7?%), Pseudomonas alcaligenes ATCC 14909(T) (97.8?%), Pseudomonas alcaliphila DSM 17744(T) (97.8?%), Pseudomonas toyotomiensis JCM 15604(T) (97.6?%), Pseudomonas oleovorans subsp. lubricantis DSM 21016(T) (97.6?%) and Pseudomonas argentinensis BCRC 17807(T) (97.5?%), and lower sequence similarity to other species of the genus Pseudomonas. According to DNA-DNA association analysis, the relatedness of strain CC-G9A(T) to P. mendocina BCRC 10458(T), P. alcaliphila DSM 17744(T), P. alcaligenes BCRC 11893(T), P. oleovorans subsp. lubricantis DSM 21016(T), P. argentinensis BCRC 17807(T) and P. oleovorans subsp. oleovorans BCRC 11902 was 55.1±3.1, 13.7±1.5, 14.1±1.8, 58.5±1.1, 28.9±2.0 and 28.6±1.8?%, respectively. The evolutionary trees reconstructed based on 16S rRNA, gyrB and rpoB gene sequences revealed varying phylogenetic neighbourhoods of strain CC-G9A(T) with regard to the most closely related type strains. The predominant quinone system was ubiquinone (Q-9) and the DNA G+C content was 64.3±1.3 mol%. The major fatty acids were C10?:?0 3-OH, C12?:?0, C12?:?0 3-OH, C16?:?0 and summed features 3 and 8 consisting of C16?:?1?7c/C16?:?1?6c and C18?:?1?7c/C18?:?1?6c, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-G9A(T) is proposed to represent a novel species within the genus Pseudomonas for which the name Pseudomonas guguanensis sp. nov. is proposed. The type strain is CC-G9A(T) (?=?BCRC 80438(T)?=?JCM 18416(T)).
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Shimia biformata sp. nov., isolated from surface seawater, and emended description of the genus Shimia Choi and Cho 2006.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 08-01-2013
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A novel, Gram-staining-negative, non-flagellated, oval or short-rod-shaped, strictly aerobic and non-spore-forming marine bacterium, designated strain CC-AMW-C(T), was isolated from coastal surface seawater in Kending County, Taiwan. Cells of strain CC-AMW-C(T) displayed unusual morphology and formed colourless or beige colonies on marine agar. The isolate shared pairwise 16S rRNA gene sequence similarities of 97.2 and 97.1?% with Shimia marina BCRC 80068(T) and Shimia isoporae BCRC 80085(T), respectively, and established a discrete phyletic lineage closely associated with the members of the genus Shimia. DNA-DNA hybridization values indicated <18.2?% genomic relatedness with species of the genus Shimia. The polar lipid profile of strain CC-AMW-C(T) comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, four unidentified lipids and an unidentified phospholipid. The predominant fatty acids were C18?:?1?7c and/or C18?:?1?6c (summed feature 8; 75.5?%). The DNA G+C content was 61.2 mol%. The predominant respiratory quinone was ubiquinone Q-10 and the major polyamine was cadaverine. The chemotaxonomic evidence, including extraordinary amounts of C18?:?1?7c and/or C18?:?1?6c, major polar lipids, polyamine, quinone and DNA G+C contents of CC-AMW-C(T), was in line with that of the members of the genus Shimia. Thus, strain CC-AMW-C(T) should be classified as a novel species of the genus Shimia, for which the name Shimia biformata sp. nov. is proposed. The type strain is CC-AMW-C(T) (?=?JCM 18818(T)?=?BCRC 80548(T)). An emended description of the genus Shimia is also proposed.
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Sphingomicrobium marinum sp. nov. and Sphingomicrobium flavum sp. nov., isolated from surface seawater, and emended description of the genus Sphingomicrobium.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 07-16-2013
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Two Gram-staining-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated and non-spore-forming amylolytic marine bacterial strains, designated CC-AMZ-30M(T) and CC-AMZ-30N(T), were isolated from coastal surface seawater in Taiwan. Strain CC-AMZ-30M(T) shared pairwise 16S rRNA gene sequence similarities of 95.8, 95.0 and <94.0?% to Sphingomicrobium lutaoense CC-TBT-3(T), Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) and other sphingomonads, respectively. Strain CC-AMZ-30N(T) shared 97.0, 96.7, 95.0 and <95.1?% similarities to strain CC-AMZ-30M(T), Sphingomicrobium lutaoense CC-TBT-3(T), Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) and other sphingomonads, respectively. The common polar lipids of the two strains include a signature glycolipid (GL2), diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and sphingoglycolipid in major amounts besides moderate-to-trace amounts of an unidentified aminolipid and several unidentified glycolipids. Both strains contained C18?:?1?7c/C18?:?1?6c, C16?:?1?7c/C16?:?1?6c, C16?:?0 and C18?:?1 2-OH as major (>5?% of the total) fatty acids. Strains CC-AMZ-30M(T) and CC-AMZ-30N(T) had DNA G+C contents of 64.2 and 65.2 mol%, respectively. The major polyamine was spermidine in strain CC-AMZ-30M(T) and triamine sym-homospermidine in strain CC-AMZ-30N(T). Both strains contained ubiquinone Q-10 as the predominant respiratory quinone. Differential phylogenetic and chemotaxonomic evidence including the presence of characteristic GL2, C18?:?1 2-OH and several other phenotypic features supported the classification of strains CC-AMZ-30M(T) and CC-AMZ-30N(T) as two novel species of the genus Sphingomicrobium, for which we propose the names Sphingomicrobium marinum sp. nov. and Sphingomicrobium flavum sp. nov., respectively; corresponding type strains are Sphingomicrobium marinum CC-AMZ-30M(T) (?=?JCM 18554(T)?=?BCRC 80466(T)) and Sphingomicrobium flavum CC-AMZ-30N(T) (?=?JCM 18555(T)?=?BCRC 80467(T)). An emended description of the genus Sphingomicrobium is also proposed.
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Zeaxanthin production by novel marine isolates from coastal sand of India and its antioxidant properties.
Appl. Biochem. Biotechnol.
PUBLISHED: 07-08-2013
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Zeaxanthin carotenoids are class of commercially important natural products and diverse biomolecules produced by plants and many microorganisms. Bacteria often produce a cocktail of polar and nonpolar carotenoids limiting their industrial applications. Marine members of the family Flavobacteriaceae are known to produce potential carotenoids such as astaxanthin and zeaxanthin. A few bacterial species have been reported for the predominant production zeaxanthin. Here, we report the molecular identification of the zeaxanthin as a major carotenoid produced by two novel bacteria (YUAB-SO-11 and YUAB-SO-45) isolated from sandy beaches of South West Coast of India and the effect of carbon sources on the production of zeaxanthin. The strains were identified based on the 16S rRNA gene sequencing as a member of genus Muricauda. The closest relatives of YUAB-SO-11 and YUAB-SO-45 were Muricauda aquimarina (JCM 11811(T)) (98.9 %) and Muricauda olearia (JCM 15563(T)) (99.2 %), respectively, indicating that both of these strains might represent a novel species. The highest level of zeaxanthin production was achieved (YUAB-SO-11, 1.20 ± 0.11 mg g(-1)) and (YUAB-SO-45, 1.02 ± 0.13 mg g(-1)) when cultivated in marine broth supplemented with 2 % NaCl (pH 7) and incubated at 30 °C. Addition of 0.1 M glutamic acid, an intermediate of citric acid cycle, enhanced the zeaxanthin production as 18 and 14 % by the strains YUAB-SO-11 and YUAB-SO-45 respectively. The zeaxanthin showed in vitro nitric oxide scavenging, inhibition of lipid peroxidation, and 2,2-diphenyl-1-picryl hydrazyl scavenging activities higher than the commercial zeaxanthin. The results of this study suggest that two novel strains YUAB-SO-11 and YUAB-SO-45 belonging to genus Muricauda produce zeaxanthin as a predominant carotenoid, and higher production of zeaxanthin was achieved on glutamic acid supplementation. The pigment showed good in vitro antioxidant activity, which can be exploited further for commercial applications.
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Description of Noviherbaspirillum malthae gen. nov., sp. nov., isolated from an oil-contaminated soil, and proposal to reclassify Herbaspirillum soli, Herbaspirillum aurantiacum, Herbaspirillum canariense and Herbaspirillum psychrotolerans as Noviherbaspi
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 06-07-2013
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An aerobic, Gram-negative, rod-shaped bacterium with polar flagella, strain CC-AFH3(T), was isolated from an oil-contaminated site located in Kaohsiung county, Taiwan. Strain CC-AFH3(T) grew at 20-40 °C, pH 5.0-10.0 and <2?% (w/v) NaCl. 16S rRNA gene sequence analysis indicated that strain CC-AFH3(T) showed the greatest degree of similarity to Herbaspirillum soli SUEMI10(T) (96.5?%), H. aurantiacum SUEMI08(T) (96.3?%), H. canariense SUEMI03(T) (96.0?%), H. psychrotolerans PB1(T) (95.4?%) and members of other Herbaspirillum species (94.1-95.2?%), and lower similarity to members of other genera (<94?%). Phylogenetic analyses also positioned the novel strain in the genus Herbaspirillum as an independent lineage. The major fatty acids in strain CC-AFH3(T) were C10?:?0 3-OH, C12?:?0, C14?:?0 2-OH, C16?:?0, iso-C15?:?0 3-OH, C17?:?0 cyclo, C16?:?1?7c/C16?:?1?6c and C18?:?1?7c/C18?:?1?6c. The major polar lipids of strain CC-AFH3(T) were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The predominant quinone was ubiquinone 8 (Q-8) and the DNA G+C content was 63.4 mol%. On the basis of 16S rRNA gene sequence analysis in combination with physiological and chemotaxonomic data, strain CC-AFH3(T) represents a novel species in a new genus, for which we propose the name Noviherbaspirillum malthae gen. nov., sp. nov.; the type strain of Noviherbaspirillum malthae is CC-AFH3(T) (?=?BCRC 80516(T)?=?JCM 18414(T)). We also propose the reclassification of Herbaspirillum soli, Herbaspirillum aurantiacum, Herbaspirillum canariense and Herbaspirillum psychrotolerans as Noviherbaspirillum soli comb. nov. (type strain SUEMI10(T)?=?LMG 26149(T)?=?CECT 7840(T)), Noviherbaspirillum aurantiacum comb. nov. (type strain SUEMI08(T)?=?LMG 26150(T)?=?CECT 7839(T)), Noviherbaspirillum canariense comb. nov. (type strain SUEMI03(T)?=?LMG 26151(T)?=?CECT 7838(T)) and Noviherbaspirillum psychrotolerans comb. nov. (type strain PB1(T)?=?DSM 26001(T)?=?LMG 27282(T)), respectively. An emended description of Herbaspirillum seropedicae is also presented.
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Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 05-03-2013
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An aerobic, Gram-stain-negative, spiral or rod-shaped, non-spore-forming, diazotrophic bacterium (strain CC-LY743(T)) was isolated from a fermentative tank in Taiwan. Strain CC-LY743(T) was able to grow at 20-37 °C and pH 6.0-8.0 and tolerated up to 3.0?% (w/v) NaCl. It was positive for nitrogen fixation, with activity of 10.6 nmol ethylene h(-1). 16S rRNA gene sequence analysis of strain CC-LY743(T) showed highest similarity to Azospirillum picis DSM 19922(T) (96.1?%), Azospirillum oryzae JCM 21588(T) (96.0?%) and Azospirillum rugosum DSM 19657(T) (96.0?%) and lower similarity (<96.0?%) to all other Azospirillum species. Highest nifH gene sequence similarities were obtained with Azospirillum brasilense BCRC 12270(T) (92.0?%), Azospirillum formosense BCRC 80273(T) (92.3?%) and A. rugosum DSM 19657(T) (91.8?%). It was positive in the rapid identification by a genus-specific primer set. The predominant quinone system was ubiquinone 10 (Q-10) and the DNA G+C content was 69.6±0.1 mol%. The major fatty acids found in strain CC-LY743(T) were n-C16?:?0, C19?:?0 cyclo ?8c, C14?:?0 3-OH/C16?:?1 iso I, C16?:?1?7c/C16?:?1?6c and C18?:?1?7c/C18?:?1?6c. Based on its phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY743(T) is considered to represent a novel species within the genus Azospirillum for which the name Azospirillum fermentarium sp. nov. is proposed. The type strain is CC-LY743(T) (?=?BCRC 80505(T)?=?JCM 18688(T)?=?LMG 27264(T)).
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Sphingomicrobium astaxanthinifaciens sp. nov., an astaxanthin-producing glycolipid-rich bacterium isolated from surface seawater and emended description of the genus Sphingomicrobium.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 03-22-2013
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A Gram-stain-negative, rod-shaped, strictly aerobic, flagellated and non-spore-forming marine bacterium designated strain CC-AMO-30B(T) was isolated from coastal surface seawater, Taiwan. Strain CC-AMO-30B(T) synthesized astaxanthin [40 µg (g dry weight)(-1)] and formed reddish-orange-coloured colonies on marine agar (Difco 2216). The strain showed highest pairwise 16S rRNA gene sequence similarity to Sphingomicrobium lutaoense CC-TBT-3(T) (96.4%) followed by other members of the family Sphingomonadaceae (<94%) and established a discrete phyletic lineage associated with the former. The polar lipid profile constituted a remarkable number of unidentified glycolipids (GL1-8), in addition to diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and two unidentified lipids (L1-2). The major fatty acids (>5% of total fatty acids) were C(18:1)?7c/C(18:1)?6c (summed feature 8), C(16:1)?7c/C(16:1)?6c (summed feature 3), C(18:1) 2-OH, methyl C(18:1)?7c, C(17:1)?6c and C(16?:?0). DNA G+C content was 70.6%; major respiratory quinone was ubiquinone Q-10; predominant polyamine was the triamine sym-homospermidine. Chemotaxonomic evidence including characteristic glycolipid profile, presence of significant amounts of C(18:1) 2-OH and absence of typical hydroxylated fatty acids such as C(14:0) 2-OH, C(15:0) 2-OH and C(16:0) 2-OH in considerable amounts, accompanied by phylogenetic distinctiveness and several other phenotypic features support the classification of strain CC-AMO-30B(T) as a representative of a novel species within the genus Sphingomicrobium for which the name Sphingomicrobium astaxanthinifaciens sp. nov. is proposed; the type strain is CC-AMO-30B(T) (?=JCM 18551(T)?=BCRC 80465(T)).
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Chiayiivirga flava gen. nov., sp. nov., a novel bacterium of the family Xanthomonadaceae isolated from an agricultural soil, and emended description of the genus Dokdonella.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 03-08-2013
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A novel Gram-reaction-negative, yellow-pigmented, aerobic, non-motile and rod-shaped bacterium designated strain CC-YHH031(T) was isolated from an agricultural soil collected at Chiayi County, Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CC-YHH031(T) formed a discrete monophyletic lineage in the family Xanthomonadaceae, sharing high pairwise sequence similarity of 93.5-95.2 and 94.8% with species of the genus Dokdonella (94.9% similarity to the type strain of the type species) and Aquimonas voraii GPTSA 20(T), respectively. The genomic DNA G+C content of strain CC-YHH031(T) was 68.6 ± 0.7 mol% and the predominant respiratory quinone was ubiquinone Q-8. Spermidine was the principal polyamine, with minor amounts of putrescine. Major fatty acids (>5% of total fatty acids) were iso-C(16:00, iso-C(15:0), C(16:1)?7c and/or C(16:1)?6c (summed feature 3), iso-C(17:1)?9c, iso-C(14:0), iso-C(11:0) and iso-C(11:0) 3-OH. The polar lipid profile of strain CC-YHH031(T) included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids (APL1-2) and four unidentified phospholipids (PL1-4). Strain CC-YHH031(T) was distinguished particularly from the type species of the genus Dokdonella (Dokdonella koreensis) by the presence of major amounts of iso-C(14:0) and summed feature 3 and minor amounts of iso-C(17:0) and by the complete absence of anteiso-C(17:0), the presence of PL1-3 and APL1-2, the absence of APL3 and the presence of putrescine in the former. On the basis of distinguishing genotypic and phenotypic evidence, strain CC-YHH031(T) is proposed to represent a novel genus and species within the family Xanthomonadaceae, for which the name Chiayiivirga flava gen. nov., sp. nov. is proposed. The type strain of Chiayiivirga flava is CC-YHH031(T) (?=BCRC 80273(T)?=DSM 24163(T)).
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Pseudomonas formosensis sp. nov., a gamma-proteobacteria isolated from food-waste compost in Taiwan.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 02-22-2013
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A taxonomic study was carried out on a novel aerobic bacterial strain, designated CC-CY503(T), isolated from food-waste compost in Taiwan. Cells were Gram-stain-negative short rods, motile by means of a monopolar flagellum. Strain CC-CY503(T) was able to grow at 20-50 °C and pH 6.0-10.0 and to tolerate <6% NaCl (w/v). Phylogenetic analysis of 16S rRNA gene sequences showed that this bacterium belonged to the genus Pseudomonas, with Pseudomonas pertucinogena ATCC 190(T) as the closest neighbour, sharing a sequence similarity of 97.9%. The DNA-DNA relatedness value of strain CC-CY503(T) with P. pertucinogena ATCC 190(T) was 37.8 ± 2.3%. The phylogenetic trees reconstructed based on gyrB and rpoB gene sequences supported the classification of strain CC-CY503(T) as a novel member of the genus Pseudomonas. The predominant quinone system was ubiquinone (Q-9) and the DNA G+C content was 63.1 ± 0.4 mol%. The major fatty acids were C(12:0), C(16:0), C(17:0) cyclo, C(19:0) cyclo ?8c and summed features 3 and 8 consisting of C(16:1)?7c/C(16:1)?6c and C(18:1)?7c/C(18:1)?6c, respectively. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. On the basis of its distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-CY503(T) (?=BCRC 80437(T)?=JCM 18415(T)) is proposed to represent a novel species within the genus Pseudomonas, for which the name Pseudomonas formosensis sp. nov. is proposed.
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Cohnella formosensis sp. nov., a xylanolytic bacterium isolated from the rhizosphere of Medicago sativa L.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 01-11-2013
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A Gram-positive, spore-forming, aerobic, rod-shaped, xylanolytic bacterium designated strain CC-Alfalfa-35(T) was isolated from the rhizosphere of Medicago sativa L. in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain CC-Alfalfa-35(T) was affiliated to the genus Cohnella. Strain CC-Alfalfa-35(T) shared 95.3 % pairwise 16S rRNA gene sequence similarity to the type strain of the type species of the genus Cohnella (Cohnella thermotolerans DSM 17683(T)) besides showing a similarity of 97.4-93.6 % with other recognized species of the genus Cohnella. The DNA-DNA hybridization value between CC-Alfalfa-35(T) and Cohnella thailandensis KCTC 22296(T) was 37.7 % ± 1.7 % (reciprocal value, 55.7 % ± 3.0 %). Predominant cellular fatty acids were iso-C16 : 0 and anteiso-C15 : 0. The polar lipid profile constituted diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lysyl-phosphatidylglycerol, three unidentified phospholipids and three unidentified aminophospholipids. The major respiratory quinone was MK-7 and the DNA G+C content was 58.3 mol%. Strain CC-Alfalfa-35(T) contained meso-diaminopimelic acid as the major diamino acid in the cell-wall peptidoglycan. Based on the polar lipid and fatty acid profiles, which were in line with those of C. thermotolerans DSM 17683(T), coupled with additional distinguishing genotypic, phenotypic and chemotaxonomic features, strain CC-Alfalfa-35(T) is proposed to represent a novel species within the genus Cohnella, for which the name Cohnella formosensis sp. nov. is proposed. The type strain is CC-Alfalfa-35(T) ( = JCM 18405(T) = BCRC 80428(T)).
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Supercritical carbon dioxide micronization of zeaxanthin from moderately thermophilic bacteria Muricauda lutaonensis CC-HSB-11T.
J. Agric. Food Chem.
PUBLISHED: 03-18-2011
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Moderately thermophilic bacterial strain CC-HSB-11(T) (Muricauda lutaonensis), which was described recently from a coastal hot spring of Green Island, Taiwan, has been identified to produce zeaxanthin as a predominant xanthophyll by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Cell culture in bioreactor produced 3.12 ± 0.18 mg zeaxanthin L(-1) of culture. Micronization of zeaxanthin was achieved through supercritical carbon dioxide antisolvent precipitation method. Yield of zeaxanthin after the process was 53.4%. Dynamic light scattering assay determined the polydisperse existence of micronized particles of size 3 nm to 2 ?m. Field emission scanning electron microscopy revealed distinct morphology and size distribution heterogeneity of particles. Integrity of zeaxanthin after the antisolvent process was assessed by LC-MS/MS. The technique capitalizes on the inherent ability of CC-HSB-11(T) to synthesize zeaxanthin and the work demonstrated feasibility of antisolvent precipitation method to produce microparticles exploiting a bacterial strain.
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Aureimonas ferruginea sp. nov. and Aureimonas rubiginis sp. nov., two siderophore-producing bacteria isolated from rusty iron plates.
Int. J. Syst. Evol. Microbiol.
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Two novel siderophore-producing strains designated CC-CFT023(T) and CC-CFT034(T), isolated from a rusty iron plate, are characterized in this study. Cells of both strains were catalase- and oxidase-positive. Strain CC-CFT023(T) showed a high pairwise 16S rRNA gene sequence similarity to Aureimonas altamirensis LMG 23375(T) (96.0 %), whereas, strain CC-CFT034(T) shared a maximum similarity to Aureimonas ureilytica DSM 18598(T) (95.8 %). The predominant quinone system was ubiquinone (Q-10) and the DNA G+C contents of CC-CFT023(T) and CC-CFT034(T) were 69.5 mol% and 67.7 mol%, respectively. The major fatty acids of these strains were C16 : 0, C18 : 0, C19 : 0 cyclo ?8c, C18 : 1 2-OH and summed features 3 and 8, consisting of C16 : 1?7c/C16 : 1?6c and C18 : 1?7c/C18 : 1?6c, respectively. Polar lipid profiles of both of these isolates include diphosphatidylglycerol (DPG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and most importantly sulfoquinovosyldiacylglycerol (SQDG), which is one of the typical molecular features reported to delineate the members of the genus Aureimonas from their closest relatives, species of the genus Aurantimonas. Based on the phylogenetic, phenotypic and chemotaxonomic features, strains CC-CFT023(T) and CC-CFT034(T) represent two novel species within the genus Aureimonas, for which the names Aureimonas ferruginea sp. nov. (type strain CC-CFT023(T) = BCRC 80439(T) = JCM 18444(T)) and Aureimonas rubiginis sp. nov. (type strain CC-CFT034(T) = BCRC 80440(T) = JCM 18445(T)) are proposed.
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Pseudomonas sagittaria sp. nov., a siderophore-producing bacterium isolated from oil-contaminated soil.
Int. J. Syst. Evol. Microbiol.
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An aerobic, Gram-stain-negative, rod-shaped bacterium with a single polar flagellum, designated CC-OPY-1(T), was isolated from an oil-contaminated site in Taiwan. CC-OPY-1(T) produces siderophores, and can grow at temperatures of 25-37 °C and pH 5.0-9.0 and tolerate <5 % (w/v) NaCl. The 16S rRNA gene sequence analysis of CC-OPY-1(T) showed high pairwise sequence similarity to Pseudomonas alcaligenes BCRC 11893(T) (97.1 %), Pseudomonas. alcaliphila DSM 17744(T) (97.1 %), Pseudomonas tuomuerensis JCM 14085(T) (97.1 %), Pseudomonas toyotomiensis JCM 15604(T) (96.9 %) and lower sequence similarity to remaining species of the genus Pseudomonas. The phylogenetic trees reconstructed based on gyrB and rpoB gene sequences supported the classification of CC-OPY-1(T) as a novel member of the genus Pseudomonas. The predominant quinone system of strain CC-OPY-1T was ubiquinone (Q-9) and the DNA G+C content was 68.4 ± 0.3 mol%. The major fatty acids were C12 : 0, C16 : 0, C17 : 0 cyclo and summed features 3 and 8 consisting of C16 : 1?7c/C16 : 1?6c and C18 : 1?7c/C18 : 1?6c, respectively. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylcholine (PC) and two unknown phospholipids (PL1-2). Due to distinct phylogenetic, phenotypic and chemotaxonomic features, CC-OPY-1(T) is proposed to represent a novel species within the genus Pseudomonas for which the name Pseudomonas sagittaria sp. nov. is proposed. The type strain is CC-OPY-1(T) ( = BCRC 80399(T) = JCM 18195(T)).
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Siansivirga zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing member of the family Flavobacteriaceae isolated from coastal seawater of Taiwan.
FEMS Microbiol. Lett.
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A strictly aerobic, Gram-negative, rod-shaped bacterium (strain CC-SAMT-1(T)) showing gliding motility was isolated from coastal seawater of China Sea, Taiwan. Strain CC-SAMT-1(T) synthesizes all-trans-zeaxanthin (6.5 ± 0.5 mg g(-1) dry biomass) as a predominant xanthophyll carotenoid. As determined by 16S rRNA gene analysis, strain CC-SAMT-1(T) shared very high sequence similarity to the members of the genera Mariniflexile (96.1-95.3%) and Gaetbulibacter (96.0-95.9%); however, it formed a distinct phyletic lineage distantly associated with Mariniflexile species. Polar lipid profile constitutes phosphatidylethanolamine, four unidentified aminolipids, four unidentified lipids, and an unidentified glycolipid. Strain CC-SAMT-1(T) contains excessive unidentified aminolipid lipid (AL2-4) and glycolipid contents, and therefore clearly distinct from Mariniflexile species. Major fatty acids (> 5% of total fatty acids) were iso-C(15:0) (14.8%), iso-C(17:0) 3-OH (11.8%), iso-C(15:1) G (10.6%), anteiso-C(15:0) (9.7%), C(16:0) (8.1%), iso-C(16:0) 3-OH (7.9%), iso-C(15:0) 3-OH (7.5%), and summed feature 3 (containing C(16:1) ?6c and/or C(16:1) ?7c) (7.5%). Menaquinone-6 (MK-6) was major respiratory quinone. DNA G+C content was 33.7 mol%. Based on polyphasic taxonomy, strain CC-SAMT-1(T) represents a novel genus and species in the family Flavobacteriaceae for which the name Siansivirga zeaxanthinifaciens gen. nov., sp. nov. is proposed. The type strain is CC-SAMT-1(T) (= BCRC 80315(T) = JCM 17682(T)).
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