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Find video protocols related to scientific articles indexed in Pubmed.
Secular changes of stature in rural children and adolescents in china, 1985-2010.
Biomed. Environ. Sci.
PUBLISHED: 12-19-2014
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To analyze and assess secular change in stature in rural children and adolescents in China from 1985 to 2010.
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Polymerization Topochemistry of Cellulose Nanocrystals: a Function of Surface Dehydration Control.
Langmuir
PUBLISHED: 11-12-2014
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The activation (dehydration) of cellulose nanocrystals (CNCs) toward surface "brush" polymerization is accomplished either by freeze-drying or solvent-exchange. However, the question of which one of these protocols to choose over the other is left to the discretion of the researcher. The current study attempts to shed light on this question by installing a standard polymer, polycaprolactone (PCL), onto the surface of both freeze-dried and solvent-exchanged CNCs by ring-opening polymerization (ROP), and studying the differences in polymerization and final product properties. The work demonstrates that the efficiency of surface polymerization and final product properties are in fact influenced by the protocols. The differences between the two sample PCL-grafted CNCs were investigated by X-ray photoelectron spectroscopy (XPS), elemental analysis, gel permeation chromatography (GPC), and contact angle measurements. The freeze-dried samples had significantly reduced PCL surface density. The crystallinity of the solvent-exchanged PCL-grafted CNCs (SECNC-g-PCL), however, was lower than that of either pure CNCs or freeze-dried PCL-grafted CNCs (FDCNC-g-PCL). It was determined that solvent-exchange sufficiently modified the CNC surface to provide enhanced reactivity, an effect that was not as apparent for FDCNC-g-PCL. The dispersibility, surface morphology, surface accessibility, aggregation, and thermal stability of the two species were also quite different. In general, it was found that solvent-exchanged CNCs are more favorable toward surface brush polymerization.
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[The new method monitoring crop water content based on NIR-Red spectrum feature space].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 11-01-2014
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Moisture content is an important index of crop water stress condition, timely and effective monitoring of crop water content is of great significance for evaluating crop water deficit balance and guiding agriculture irrigation. The present paper was trying to build a new crop water index for winter wheat vegetation water content based on NIR-Red spectral space. Firstly, canopy spectrums of winter wheat with narrow-band were resampled according to relative spectral response function of HJ-CCD and ZY-3. Then, a new index (PWI) was set up to estimate vegetation water content of winter wheat by improveing PDI (perpendicular drought index) and PVI (perpendicular vegetation index) based on NIR-Red spectral feature space. The results showed that the relationship between PWI and VWC (vegetation water content) was stable based on simulation of wide-band multispectral data HJ-CCD and ZY-3 with R2 being 0.684 and 0.683, respectively. And then VWC was estimated by using PWI with the R2 and RMSE being 0.764 and 0.764, 3.837% and 3.840%, respectively. The results indicated that PWI has certain feasibility to estimate crop water content. At the same time, it provides a new method for monitoring crop water content using remote sensing data HJ-CCD and ZY-3.
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Clinical Management and Outcomes in Patients with Hyperfunctioning Distant Metastases from Differentiated Thyroid Cancer After Total Thyroidectomy and Radioactive Iodine Therapy.
Thyroid
PUBLISHED: 10-22-2014
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Background: Hyperfunctioning distant metastasis (HFDM) from differentiated thyroid cancer (DTC) is a rare entity. This study aimed to assess the outcomes of DTC patients presenting with HFDM after total thyroidectomy and radioactive iodine therapy. Methods: A total of 5367 DTC patients treated with (131)I after total thyroidectomy were analyzed retrospectively from January 1991 to June 2013. Therapeutic efficacy was evaluated based on changes in serum thyroglobulin (Tg) and anatomical imaging changes in metastatic lesions. The relationships between survival time and several variables were assessed by univariate and multivariate analyses using the Kaplan-Meier method and Cox's proportional hazards model respectively. Results: Thirty-eight patients with HFDM from DTC were diagnosed, including four with hyperthyroidism, four with subclinical hyperthyroidism, and three with subclinical hypothyroidism. The remaining 27 were euthyroid. Of 25 patients with lung metastases, 84% (21/25) showed disappearance or shrinkage of lung nodules; of 24 patients with bone metastases, 66.67% (16/24) exhibited no obvious imaging changes in metastatic bone lesions after (131)I therapy. Serum Tg decreased significantly in 81.58% (31/38) and increased in 18.42% (7/38) after (131)I therapy. The 10-year survival rate of DTC patients with HFDM was 65.79% (25/38). Multivariate analyses identified age at occurrence of distant metastases (<45 years), only lung metastases, and papillary thyroid cancer (PTC; p=0.032, NA, and 0.043) as independent predictors of survival. Conclusion: The response of hyperfunctioning lung metastases to (131)I treatment was better than that of non-hyperfunctioning lung metastases in DTC, while hyperfunctioning bone metastases responded similarly compared to non-hyperfunctioning bone metastases. Patients younger than 45 years at occurrence of distant metastases, those with only lung metastases, and patients with PTC had better prognoses.
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Performance of 18F-Fluoride PET or PET/CT for the Detection of Bone Metastases: A Meta-analysis.
Clin Nucl Med
PUBLISHED: 10-08-2014
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This study aimed to evaluate the diagnostic accuracy of F-fluoride PET or PET/CT compared with Tc-MDP bone scintigraphy and F-FDG PET/CT in the detection of bone metastases.
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[Quantitative analysis of Cu in water by collinear DP-LIBS].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-02-2014
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The purpose of this research is to study the influence of double pulse laser induced breakdown spectroscopy (DP-LIBS) on the sensitivity of Cu in water. The water solution of Cu was tested by collinear DP-LIBS in this article. The results show that spectral intensity of Cu can be enhanced obviously by DP-LIBS, compared with single pulse laser induced breakdown spectroscopy (SP-LIBS). Besides, the experimental results were significantly impacted by delay time between laser pulse and spectrometer acquisition, delay time of double laser pulse and energy of laser pulse and so on. The paper determined the best conditions for DP-LIBS detecting Cu in water. The optimal acquisition delay time was 1 380 ns. The best laser pulse delay time was 25 ns. The most appropriate energy of double laser pulse was 100 mJ. Characteristic analysis of spectra of Cu at 324.7 and 327.4 nm was done for quantitative analysis. The detection limit was 3.5 microg x mL(-1) at 324.7 nm, and the detection limit was 4.84 microg x mL(-1) at 327.4 nm. The relative standard deviation of the two characteristic spectral lines was within 10%. The calibration curve of characteristic spectral line, established by 327.4 nm, was verified with 500 microg x mL(-1) sample. Concentration of the sample was 446 microg x mL(-1) calculated by the calibration curve. This research shows that the detection sensitivity of Cu in water can be improved by DP-LIBS. At the same time, it had high stability.
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[Efficacy observation of post-stroke dysphagia treated with acupuncture at Lianquan (CV 23)].
Zhongguo Zhen Jiu
PUBLISHED: 09-20-2014
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To verify the clinical efficacy on post-stroke dysphagia treated with acupuncture at Lianquan (CV 23).
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Foxo1 Inhibits Diabetic Mucosal Wound Healing but Enhances Healing of Normoglycemic Wounds.
Diabetes
PUBLISHED: 09-03-2014
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Re-epithelialization is an important part in mucosal wound healing. Surprisingly little is known about the impact of diabetes on the molecular events of mucosal healing. We examined the role of the transcription factor forkhead box O1 (Foxo1) in oral wounds of diabetic and normoglycemic mice with keratinocyte specific Foxo1 deletion. Diabetic mucosal wounds had significantly delayed healing with reduced cell migration and proliferation. Foxo1 deletion rescued the negative impact of diabetes on healing but had the opposite effect in normoglycemic mice. Diabetes in vivo and high glucose in vitro enhanced expression of chemokine (C-C motif) ligand 20 (CCL20) and interleukin-36? (IL-36?) in a Foxo1 dependent manner. High glucose stimulated Foxo1 binding to CCL20 and IL36? promoters and CCL20 and IL-36? significantly inhibited migration of these cells in high glucose. In normal healing Foxo1 was needed for transforming growth factor-?1 (TGF-?1) expression and in standard glucose TGF-?1 rescued the negative effect of Foxo1 silencing on migration in vitro. We propose that Foxo1 under diabetic or high glucose conditions impairs healing by promoting high levels of CCL20 and IL-36? expression but under normal conditions enhances it by inducing TGF-?1. This finding provides mechanistic insight into how Foxo1 mediates the impact of diabetes on mucosal wound healing.
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Dual-phase (99m)Tc-MIBI scintigraphy with delayed neck and thorax SPECT/CT and bone scintigraphy in patients with primary hyperparathyroidism: correlation with clinical or pathological variables.
Ann Nucl Med
PUBLISHED: 08-15-2014
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The purpose of this study was to assess the relationship between (99m)Tc-MIBI and (99m)Tc-MDP bone scintigraphy and clinical or pathological variables, including preoperative serum PTH levels and tumor diameter, in patients with newly diagnosed PHPT.
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Thickness of soft tissue of lower extremities measured with magnetic resonance imaging as a new indicator for staging unilateral secondary lower extremity lymphedema.
Acta Radiol
PUBLISHED: 08-08-2014
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Chronic progressive swelling of the lower extremity due to secondary lower extremity lymphedema (LEL) can affect a patient's quality of life, both physically and psychologically. A feasible and reproducible method for detecting and staging LEL will facilitate decision-making about appropriate management strategies.
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Right pulmonary artery atresia with left circumflex coronary collaterals supplying the affected lung diagnosed by V/Q scintigraphy and CTA: a case report and review of the literature.
Ann Nucl Med
PUBLISHED: 08-08-2014
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Ventilation-perfusion (V/Q) lung scintigraphy has been a popular diagnostic test for evaluation of pulmonary thromboembolism (PE) for almost 40 years. Despite the validation of V/Q scintigraphy, it is important to remember that there are causes of unmatched perfusion defects that are not due to PE. Here, we describe a very rare case of right main pulmonary artery atresia with left circumflex coronary collaterals supplying the affected lung in an adult patient diagnosed by V/Q scan and CT angiography (CTA). Lung perfusion scan disclosed the total absence of perfusion in the right lung, while ventilation scan disclosed decreased size of the right lung with diminished but homogeneous radioactivity distribution. CTA showed occlusion of the right main pulmonary artery with no evidence of embolus. Three-dimensional reconstruction demonstrated large, tortuous collateral vessels arising from the left circumflex coronary branch to the affected lung indicating collaterals formed from the coronary circulation to the pulmonary circulation. We highlight that demonstrations on V/Q scintigraphy in such cases should be interpreted with caution.
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Validity of soft-tissue thickness of calf measured using MRI for assessing unilateral lower extremity lymphoedema secondary to cervical and endometrial cancer treatments.
Clin Radiol
PUBLISHED: 08-06-2014
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To determine whether soft-tissue thickness of the calf measured using MRI could be valid for assessing unilateral lower extremity lymphoedema (LEL) secondary to cervical and endometrial cancer treatments.
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Log-transformation and its implications for data analysis.
Shanghai Arch Psychiatry
PUBLISHED: 08-06-2014
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The log-transformation is widely used in biomedical and psychosocial research to deal with skewed data. This paper highlights serious problems in this classic approach for dealing with skewed data. Despite the common belief that the log transformation can decrease the variability of data and make data conform more closely to the normal distribution, this is usually not the case. Moreover, the results of standard statistical tests performed on log-transformed data are often not relevant for the original, non-transformed data.We demonstrate these problems by presenting examples that use simulated data. We conclude that if used at all, data transformations must be applied very cautiously. We recommend that in most circumstances researchers abandon these traditional methods of dealing with skewed data and, instead, use newer analytic methods that are not dependent on the distribution the data, such as generalized estimating equations (GEE).
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Extracting work from magnetic-field-coupled Brownian particles.
Phys Rev E Stat Nonlin Soft Matter Phys
PUBLISHED: 08-01-2014
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Thermodynamics of the magnetic-field-coupled Brownian particles is studied. We show that in the presence of the magnetic field, work can be extracted from the reservoir even when the measurement operation and the potential change operation are applied in different spatial directions. In particular, we show that more work can be extracted if the measurements are applied in two different directions simultaneously. In all these cases, we show that the generalized second law involving the measurement information and potential change is satisfied. In addition, we show how the continuous potential change and measurement position affect the work extraction.
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[Influence of goethite on anaerobic fermentation of organic fraction of municipal solid waste (OFMSW)].
Huan Jing Ke Xue
PUBLISHED: 07-25-2014
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Effects of goethite on the anaerobic fermentation process of organic fraction of municipal solid wastes, including the biogas production and characteristics of leachate, were investigated in the anaerobic digesters constructed by PVC. The results showed that the addition of goethite promoted hydrolysis and acidogenesis efficiency of solid wastes and gas production rate. The total gas volume was 163.4 L,which increased by 20% compared to the blank. The intermediate products of Fe2+ , NH+4 -N, NO-3 -N, COD and organic acids in the leachate were analyzed. Results showed that the addition of goethite reduced the system ORP to - 124 mV which could improve the activity of the anaerobic microorganism. Addition of goethite could also promote the utilization of organic acids which decreased the inhibition effects of organic acids on the anaerobic microorganism.
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Magnetic resonance imaging for the normal mesostenium and involvement of the mesostenium in acute pancreatitis.
Biomed Res Int
PUBLISHED: 07-20-2014
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The main point of this paper is to study MRI findings of the normal mesostenium and the involvement of the mesostenium in acute pancreatitis and to discuss the relationship between the involvement of the mesostenium and the severity of acute pancreatitis. In clinical practice, the mesenterical involvement in acute pancreatitis was often observed on MRI in daily works, which was little recorded in the reported studies. We conducted the current study to assess the mesenterical involvement in acute pancreatitis with MRI. We found that the mesenterical involvement of acute pancreatitis patients is common on MRI. The mesenterical involvement has a positive correlation with the MR severity index and the Acute Physiology and Chronic Healthy Evaluation II scoring system. It has been shown that MR can be used to visualize mesenterical involvement, which is a supplementary indicator in evaluating the severity of acute pancreatitis and local and systemic complications.
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[Comparison of genetic damage in mice exposed to black carbon and ozone-oxidized black carbon].
Beijing Da Xue Xue Bao
PUBLISHED: 06-20-2014
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To make an assessment on the genotoxicity caused by black carbon (BC) and ozonized black carbon (O?-BC).
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Effects of repeated Cr(VI) intratracheal instillation on club (Clara) cells and activation of nuclear factor-kappa B pathway via oxidative stress.
Toxicol. Lett.
PUBLISHED: 06-13-2014
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Hexavalent chromium [Cr(VI)] exposure is known to induce respiratory inflammation and contribute to lung cancer development, but little is known about its target cell type in lung. In the current study, we investigated the effects of repeated Cr(VI) intratracheal instillation on club (Clara) cells and club (Clara) cell secretory protein (CC16) in rats and explored whether the nuclear factor-kappa B (NF-?B) related pathway was involved. We also studied the role of orally delivered Zn against Cr-induced adverse health effects. For four weeks, sixty Sprague-Dawley male rats received weekly intratracheal instillation of potassium dichromate (K2Cr2O7) at 0, 0.063 and 0.630mgCr/kg with or without daily intragastric administration of zinc sulfate (ZnSO4) at 10mg Zn/kg. Results showed that exposure to Cr(VI) significantly increased the organ coefficient of lung (organ weight as a percentage of body weight), albumin and total protein level in bronchoalveolar lavage fluid (BALF), indicating lung injury and compromised bronchoalveolar/blood barrier (BA/BB) integrity. With increasing Cr(VI) dose, the secretion of CC16 decreased in a dose-dependent manner, suggesting that CC16 can serve as a peripheral biomarker for club cell damage during early lung injury induced by Cr(VI). Increased expression of NF-?B were observed in club cells in both Cr-exposed groups, indicating upregulation of NF-?B, which can be induced by reactive oxygen species (ROS) generated by club cells during Cr reduction with repetitive Cr(VI) exposure. Cr-induced DNA damage was also observed, as significant increase of 8-OHdG was found with Cr exposure at 0.630mg/kg week. Oral Zn supplementation did not alleviate changes in serum CC16 level under Cr(VI) exposure, indicating its failure in protecting against Cr(VI)-induced club cell damage.
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Robust multi-type plasmid modifications based on isothermal in vitro recombination.
Gene
PUBLISHED: 06-13-2014
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A robust strategy for multi-type plasmid modifications is developed based on the isothermal in vitro recombination technology, by which any combination of the sequence modifications can be efficiently achieved in plasmids at any desired position in a seamless manner. As an example, we showed that a plasmid modification with insertion of a GFP gene, deletion of a 623-bp fragment, and substitution of an ampicillin resistance gene by a kanamycin resistance gene was accomplished simultaneously by this method. Therefore, the isothermal in vitro recombination-based multi-type plasmid modification strategy is a useful approach for broad application prospects in molecular biology studies.
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Stepwise and cooperative assembly of a cytokinetic core complex in Saccharomyces cerevisiae.
J. Cell. Sci.
PUBLISHED: 06-03-2014
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Actomyosin ring (AMR) contraction and the synthesis of an extracellular septum are interdependent pathways that mediate cytokinesis in the yeast Saccharomyces cerevisiae and other eukaryotes. How these interdependent pathways are physically connected is central for understanding cytokinesis. The yeast IQGAP (Iqg1p) belongs to the conserved AMR. The F-BAR-domain-containing protein Hof1p is a member of a complex that stimulates cell wall synthesis. We report here on the stepwise formation of a physical connection between both proteins. The C-terminal IQ-repeats of Iqg1p first bind to the essential myosin light chain before both proteins assemble with Hof1p into the Mlc1p-Iqg1p-Hof1p (MIH) bridge. Mutations in Iqg1p that disrupt the MIH complex alter Hof1p targeting to the AMR and impair AMR contraction. Epistasis analyses of two IQG1 alleles that are incompatible with formation of the MIH complex support the existence and functional significance of a large cytokinetic core complex. We propose that the MIH complex acts as hinge between the AMR and the proteins involved in cell wall synthesis and membrane attachment.
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Retrograde labeling of retinal ganglion cells in adult zebrafish with fluorescent dyes.
J Vis Exp
PUBLISHED: 05-20-2014
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As retrograde labeling retinal ganglion cells (RGCs) can isolate RGCs somata from dying sites, it has become the gold standard for counting RGCs in RGCs survival and regeneration experiments. Many studies have been performed in mammalian animals to research RGCs survival after optic nerve injury. However, retrograde labeling of RGCs in adult zebrafish has not yet been reported, though some alternative methods can count cell numbers in retinal ganglion cell layers (RGCL). Considering the small size of the adult zebrafish skull and the high risk of death after drilling on the skull, we open the skull with the help of acid-etching and seal the hole with a light curing bond, which could significantly improve the survival rate. After absorbing the dyes for 5 days, almost all the RGCs are labeled. As this method does not need to transect the optic nerve, it is irreplaceable in the research of RGCs survival after optic nerve crush in adult zebrafish. Here, we introduce this method step by step and provide representative results.
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Efficacy and safety of selumetinib compared with current therapies for advanced cancer: a meta-analysis.
Asian Pac. J. Cancer Prev.
PUBLISHED: 04-11-2014
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Selumetinib is a promising and interesting targeted therapy agent as it may reverse radioiodine uptake in patients with radioiodine-refractory differentiated thyroid cancer. We conduct this meta- analysis to compare the efficacy and safety of selumetinib with current therapies in patients with advanced cancer.
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Species-specific alternative splicing leads to unique expression of sno-lncRNAs.
BMC Genomics
PUBLISHED: 04-03-2014
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Intron-derived long noncoding RNAs with snoRNA ends (sno-lncRNAs) are highly expressed from the imprinted Prader-Willi syndrome (PWS) region on human chromosome 15. However, sno-lncRNAs from other regions of the human genome or from other genomes have not yet been documented.
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Causal inference for community-based multi-layered intervention study.
Stat Med
PUBLISHED: 04-02-2014
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Estimating causal treatment effect for randomized controlled trials under post-treatment confounding, that is, noncompliance and informative dropouts, is becoming an important problem in intervention/prevention studies when the treatment exposures are not completely controlled. When confounding is present in a study, the traditional intention-to-treat approach could underestimate the treatment effect because of insufficient exposure of treatment. In the recent two decades, many papers have been published to address such confounders to investigate the causal relationship between treatment and outcome of interest based on different modeling strategies. Most of the existing approaches, however, are suitable only for standard experiments. In this paper, we propose a new class of structural functional response model to address post-treatment confounding in complex multi-layered intervention studies within a longitudinal data setting. The new approach offers robust inference and is readily implemented. We illustrate and assess the performance of the proposed structural functional response model using both real and simulated data.
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Treatment effectiveness of Fränkel function regulator on the Class III malocclusion: a systematic review and meta-analysis.
Am J Orthod Dentofacial Orthop
PUBLISHED: 04-01-2014
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The Fränkel function regulator III appliance (FR-3) has been used to correct Class III malocclusions for many years; however, its treatment effectiveness is controversial. In this study, we aimed to assess the effectiveness of the FR-3 in treating patients with Class III malocclusion in the growth and development period.
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Mycobacterium tuberculosis serine protease Rv3668c can manipulate the host-pathogen interaction via Erk-NF-?B axis-mediated cytokine differential expression.
J. Interferon Cytokine Res.
PUBLISHED: 03-31-2014
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Tuberculosis caused by Mycobacterium tuberculosis (MTB) remains a serious global public health concern. About one-third of the global population has been latently infected with this pathogen. MTB proteases are important virulence factors and involve in subverting the host immunity. MTB protease Rv3668c was implicated in drug action and dormancy by Gene Expression Omnibus data. To define the role of Rv3668c in pathogen-host interaction, we constructed recombinant strain Mycobacterium smegmatis-Rv3668c (Ms-Rv3668c). The resultant strains were used to challenge the human macrophage cell line U937. The cytokine levels and the survival of recombinants and macrophages were monitored. The results showed that recombinant Ms-Rv3668c specifically upregulated the secretion of proinflammatory cytokines TNF-?, IL-1?, and IL-6 and downregulated the secretion of anti-inflammatory cytokine IL-10 by U937 cells, consistent with the upregulated transcription of TNF-? and IL-1?. Rv3668c recombinants demonstrated prolonged survival within the U937 cells and accelerated the death of the host cells. Inhibitor experiments showed that the ERK-NF-?B axis was involved in the Rv3668c-triggered TNF-? and IL-1? changes. These results provided evidence for the engagement of Rv3668c in the interaction between Mycobacterium and host.
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Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus.
Cell Res.
PUBLISHED: 03-25-2014
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The human 8q24 gene desert contains multiple enhancers that form tissue-specific long-range chromatin loops with the MYC oncogene, but how chromatin looping at the MYC locus is regulated remains poorly understood. Here we demonstrate that a long noncoding RNA (lncRNA), CCAT1-L, is transcribed specifically in human colorectal cancers from a locus 515 kb upstream of MYC. This lncRNA plays a role in MYC transcriptional regulation and promotes long-range chromatin looping. Importantly, the CCAT1-L locus is located within a strong super-enhancer and is spatially close to MYC. Knockdown of CCAT1-L reduced long-range interactions between the MYC promoter and its enhancers. In addition, CCAT1-L interacts with CTCF and modulates chromatin conformation at these loop regions. These results reveal an important role of a previously unannotated lncRNA in gene regulation at the MYC locus.
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A functional peptidoglycan hydrolase characterized from T4SS in 89K pathogenicity island of epidemic Streptococcus suis serotype 2.
BMC Microbiol.
PUBLISHED: 03-20-2014
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Streptococcus suis serotype 2 (S. suis 2) has evolved efficient mechanisms to cause streptococcal toxic shock syndrome (STSS), which is a new emerging infectious disease linked to S. suis. We have previously reported that a type IV secretion system (T4SS) harbored by the specific 89K pathogenicity island (PAI) of S. suis 2 contributes to the development of STSS and mediates horizontal transfer of 89K. However, the 89K T4SS machinery assembly in vivo and in vitro is poorly understood, and the component acting directly to digest the bacterial cell wall needs to be identified.
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The effects of Glycyrrhizae uralenis and its major bioactive components on pharmacokinetics of daphnetin in Cortex daphnes in rats.
J Ethnopharmacol
PUBLISHED: 03-15-2014
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Glycyrrhizae uralenis (GU) is often prescribed together with Cortex daphnes (CD) in traditional Chinese medicinal practice to increase the efficacy of CD on the treatment of rheumatoid arthritis (RA), but the reasons were still unknown. In order to clarify the rationality of herbaceous compatibility between CD and GU, the comparative evaluations on pharmacokinetic behaviors of daphnetin (a predominantly active ingredient in CD) after intragastric administration of CD and CD-GU (combination of CD and GU) extract were studied. In addition, the effects of glycyrrhizin and liquiritin, active ingredients of Glycyrrhiza triterpenes and Glycyrrhiza flavones respectively, on the pharmacokinetics of daphnetin were also investigated.
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Circulating hematopoietic stem and progenitor cells are myeloid-biased in cancer patients.
Proc. Natl. Acad. Sci. U.S.A.
PUBLISHED: 03-03-2014
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Cancer is associated with a profound perturbation in myelopoiesis that results in the accumulation of myeloid-derived suppressor cells (MDSCs) to promote disease progression. Recent studies in mice suggest that tumor-derived factors could regulate the differentiation of hematopoietic stem and progenitor cells (HSPCs) in the bone marrow and subsequently contribute to dysregulation of hematopoiesis. However, the nature and role of HPSCs in patients with cancer remain unknown. Here we show, in detailed studies of the peripheral blood from 133 untreated patients with seven different types of tumors, that the composition of circulating HSPCs was significantly altered in patients with solid tumors. The frequencies of circulating granulocyte-monocyte progenitors (GMPs) were increased four to seven fold in all types of tumors examined, and the circulating hematopoietic precursors exhibited myeloid bias with a skew toward granulocytic differentiation in patients with solid tumors. These myeloid precursors are selectively enriched in tumor tissues, and the high levels of circulating GMPs were positively correlated with disease progression. By using cord blood-derived CD34(+) cells, we developed an in vitro short-term culture model to effectively induce the rapid generation of MDSCs. We found that, among the factors produced by various tumors, GM-CSF, granulocyte colony-stimulating factor, and IL-6 could not only promote the myeloid-biased differentiation, but also induce the differentiation of myeloid precursors into functional MDSCs. These findings suggest that the altered circulating HSPCs may serve as an important link between dysregulated bone marrow hematopoiesis and accumulated MDSCs in patients with cancer.
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Competitive adsorption of heavy metal by extracellular polymeric substances (EPS) extracted from sulfate reducing bacteria.
Bioresour. Technol.
PUBLISHED: 02-24-2014
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Competitive adsorption of heavy metals by extracellular polymeric substances (EPS) extracted from Desulfovibrio desulfuricans was investigated. Chemical analysis showed that different EPS compositions had different capacities for the adsorption of heavy metals which was investigated using Cu(2+) and Zn(2+). Batch adsorption tests indicated that EPS had a higher combined ability with Zn(2+) than Cu(2+). This was confirmed and explained by Fourier transform infrared (FTIR) and excitation-emission matrix (EEM) spectroscopy analysis. FTIR analysis showed that both polysaccharides and protein combined with Zn(2+) while only protein combined with Cu(2+). EEM spectra further revealed that tryptophan-like substances were the main compositions reacted with the heavy metals. Moreover, Zn(2+) had a higher fluorescence quenching ability than Cu(2+).
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Genotoxic evaluation of titanium dioxide nanoparticles in vivo and in vitro.
Toxicol. Lett.
PUBLISHED: 02-17-2014
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With the extensive application of titanium dioxide (TiO2) nanoparticles (NPs) in food industry, there is a rising debate concerning the possible risk associated with exposure to TiO2 NPs. The purpose of this study is to evaluate the genotoxicity of TiO2 NPs using in vivo and in vitro test systems. In vivo study, the adult male Sprague-Dawley rats were exposed to anatase TiO2 NPs (75 ± 15 nm) through intragastric administration at 0, 10, 50 and 200mg/kg body weight every day for 30 days. The ?-H2AX assay showed TiO2 NPs could induce DNA double strand breaks in bone marrow cells after oral administration. However, the micronucleus test revealed that the oral-exposed TiO2 NPs did not cause damage to chromosomes or mitotic apparatus observably in rat bone marrow cells. In vitro study, Chinese hamster lung fibroblasts (V79 cells) were exposed to TiO2 NPs at the dose of 0, 5, 10, 20, 50 and 100 ?g/mL. Significant decreases in cell viability were detected in all the treated groups after 24h and 48h exposure. Significant DNA damage was only observed at the concentration of 100 ?g/mL after 24h treatment using the comet assay. The obvious gene mutation was observed at the concentration of 20 and 100 ?g/mL after 2h treatment using hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene mutation assay. This study presented a comprehensive genotoxic evaluation of TiO2 NPs, and TiO2 NPs were shown to be genotoxic both in vivo and in vitro tests. The gene mutation and DNA strand breaks seem to be more sensitive genetic endpoints for the detection of TiO2 NPs induced genotoxic effects.
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GRE T2?-weighted MRI: principles and clinical applications.
Biomed Res Int
PUBLISHED: 02-10-2014
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The sequence of a multiecho gradient recalled echo (GRE) T2*-weighted imaging (T2*WI) is a relatively new magnetic resonance imaging (MRI) technique. In contrast to T2 relaxation, which acquires a spin echo signal, T2* relaxation acquires a gradient echo signal. The sequence of a GRE T2*WI requires high uniformity of the magnetic field. GRE T2*WI can detect the smallest changes in uniformity in the magnetic field and can improve the rate of small lesion detection. In addition, the T2* value can indirectly reflect changes in tissue biochemical components. Moreover, it can be used for the early diagnosis and quantitative diagnosis of some diseases. This paper reviews the principles and clinical applications as well as the advantages and disadvantages of GRE T2*WI.
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The effect of superparamagnetic iron oxide with iRGD peptide on the labeling of pancreatic cancer cells in vitro: a preliminary study.
Biomed Res Int
PUBLISHED: 02-10-2014
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The iRGD peptide loaded with iron oxide nanoparticles for tumor targeting and tissue penetration was developed for targeted tumor therapy and ultrasensitive MR imaging. Binding of iRGD, a tumor homing peptide, is mediated by integrins, which are widely expressed on the surface of cells. Several types of small molecular drugs and nanoparticles can be transfected into cells with the help of iRGD peptide. Thus, we postulate that SPIO nanoparticles, which have good biocompatibility, can also be transfected into cells using iRGD. Despite the many kinds of cell labeling studies that have been performed with SPIO nanoparticles and RGD peptide or its analogues, only a few have applied SPIO nanoparticles with iRGD peptide in pancreatic cancer cells. This paper reports our preliminary findings regarding the effect of iRGD peptide (CRGDK/RGPD/EC) combined with SPIO on the labeling of pancreatic cancer cells. The results suggest that SPIO with iRGD peptide can enhance the positive labeling rate of cells and the uptake of SPIO. Optimal functionalization was achieved with the appropriate concentration or concentration range of SPIO and iRGD peptide. This study describes a simple and economical protocol to label panc-1 cells using SPIO in combination with iRGD peptide and may provide a useful method to improve the sensitivity of pancreatic cancer imaging.
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Patterns of lymph node recurrence after radical surgery impacting on survival of patients with pT1-3N0M0 thoracic esophageal squamous cell carcinoma.
J. Korean Med. Sci.
PUBLISHED: 01-28-2014
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The aim of this study was to investigate how patterns of lymph nodes recurrence after radical surgery impact on survival of patients with pT1-3N0M0 thoracic esophageal squamous cell carcinoma. One hundred eighty consecutive patients with thoracic esophageal squamous cell carcinoma underwent radical surgery, and the tumors were staged as pT1-3N0M0 by postoperative pathology. Lymph nodes recurrence was detected with computed tomography 3-120 months after the treatment. The patterns of lymph nodes recurrence including stations, fields and locations of recurrent lymph nodes, and impacts on patterns of survival were statistically analyzed. There was a decreasing trend of overall survival with increasing stations or fields of postoperative lymph nodes involved (all P<0.05). Univariate analysis showed that stations or fields of lymph nodes recurrence, and abdominal or cervical lymph nodes involved were prognostic factors for survival (all P<0.05). Cox analyses revealed that the field was an independent factor (P<0.05, odds ratio=2.73). Lymph nodes involved occurred predominantly in cervix and upper mediastinum (P<0.05). In conclusion, patterns of lymph node recurrence especially the fields of lymph nodes involved are significant prognostic factors for survival of patients with pT1-3N0M0 thoracic esophageal squamous cell carcinoma.
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Epithelial-mesenchymal transition involved in pulmonary fibrosis induced by multi-walled carbon nanotubes via TGF-beta/Smad signaling pathway.
Toxicol. Lett.
PUBLISHED: 01-27-2014
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Multi-walled carbon nanotubes (MWCNT) are a typical nanomaterial with a wide spectrum of commercial applications. Inhalation exposure to MWCNT has been linked with lung fibrosis and mesothelioma-like lesions commonly seen with asbestos. In this study, we examined the pulmonary fibrosis response to different length of MWCNT including short MWCNT (S-MWCNT, length=350-700nm) and long MWCNT (L-MWCNT, length=5-15?m) and investigated whether the epithelial-mesenchymal transition (EMT) occurred during MWCNT-induced pulmonary fibrosis. C57Bl/6J male mice were intratracheally instilled with S-MWCNT or L-WCNT by a single dose of 60?g per mouse, and the progress of pulmonary fibrosis was evaluated at 7, 28 and 56 days post-exposure. The in vivo data showed that only L-MWCNT increased collagen deposition and pulmonary fibrosis significantly, and approximately 20% of pro-surfactant protein-C positive epithelial cells transdifferentiated to fibroblasts at 56 days, suggesting the occurrence of EMT. In order to understand the mechanism, we used human pulmonary epithelial cell line A549 to investigate the role of TGF-?/p-Smad2 signaling pathway in EMT. Our results showed that L-MWCNT downregulated E-cadherin and upregulated ?-smooth muscle actin (?-SMA) protein expression in A549 cells. Taken together, both in vivo and in vitro study demonstrated that respiratory exposure to MWCNT induced length dependent pulmonary fibrosis and epithelial-derived fibroblasts via TGF-?/Smad pathway.
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PAHs in sediment cores at main river estuaries of Chaohu Lake: implication for the change of local anthropogenic activities.
Environ Sci Pollut Res Int
PUBLISHED: 01-26-2014
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In the present study, 28 polycyclic aromatic hydrocarbons (PAHs) were investigated in four sediment cores collected from the main river estuaries of Chaohu Lake, one of the severely polluted lakes in China. The results indicate that elevated concentrations of total PAHs (?28PAH) were found in the samples from the estuary of Nanfei River (ENF), considering BaP-based total toxicity equivalent (TEQ-BaP) and toxic unit (TU) results; there are potential adverse environmental implications. The total organic carbon (TOC) played an important role on the accumulation of PAHs at ENF and the estuary of Tongyang River (ETY). The predominant PAHs are high molecular weight (HMW) homologous for all samples; as a result, industrial wastewater from a steel company is expectedly the key source of PAHs in ENF, while coke consumption would be the important source of PAHs at other three sampling sites. Vertical distribution of PAHs in the sediment cores could be explained by the local social and economic activities. Furthermore, a minor variation of PAH composition in the sediment core could be justified by the stable structure of energy consumption in the Anhui Province. These results justify the need for further enhancement of industrial wastewater treatment and development of renewable energies which are the key factors on the control of PAH pollution in China.
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XRCC3 polymorphisms are associated with the risk of developing radiation-induced late xerostomia in nasopharyngeal carcinoma patients treated with intensity modulation radiated therapy.
Jpn. J. Clin. Oncol.
PUBLISHED: 01-22-2014
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The incidence of radiation-induced late xerostomia varies greatly in nasopharyngeal carcinoma patients treated with radiotherapy. The single-nucleotide polymorphisms in genes involved in DNA repair and fibroblast proliferation may be correlated with such variability. The purpose of this paper was to evaluate the association between the risk of developing radiation-induced late xerostomia and four genetic polymorphisms: TGF?1 C-509T, TGF?1 T869C, XRCC3 722C>T and ATM 5557G>A in nasopharyngeal carcinoma patients treated with Intensity Modulation Radiated Therapy.
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In vivo antibacterial activity of MRX-I, a new oxazolidinone.
Antimicrob. Agents Chemother.
PUBLISHED: 01-06-2014
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MRX-I is a potent oxazolidinone antibiotic against Gram-positive pathogens, including methicillin-resistant Staphylococcus aureus (MRSA), penicillin-resistant Streptococcus pneumoniae (PRSP), penicillin-intermediate S. pneumoniae (PISP), and vancomycin-resistant enterococci (VRE). In this study, the in vivo efficacy of orally administered MRX-I was evaluated using linezolid as a comparator. MRX-I showed the same or better efficacy than linezolid in both systemic and local infection models against the tested strains.
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Preparation and adsorption of bovine serum albumin-imprinted polyacrylamide hydrogel membrane grafted on non-woven polypropylene.
Talanta
PUBLISHED: 01-03-2014
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Bovine serum albumin (BSA) imprinted polypropylene (PP) fiber-grafted polyacrylamide (PAM) hydrogel membrane (PP-g-PAM MIP) was prepared using non-woven PP fiber as matrix, BSA as template molecule, and acrylamide (AM) as functional monomer via UV radiation-reduced polymerization in an aqueous phase. SEM, FT-IR, DSC and TG were used to characterize the PP grafted PAM hydrogel. Influence factors on the adsorption capacity of PP-g-PAM MIP were investigated, such as monomer concentration, cross-linker concentration, template molecule amount and pH values in BSA solution. The adsorption and recognition properties of PP-g-PAM MIP were evaluated and the results showed that the PP-g-PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA as compared with non-imprinted ones. PP-g-PAM MIPs could recognize the template protein using Lys, Ova, BHb, and Glo as control proteins, and the selectivity factor (?) was above 2.0. The imprinting efficiency of PP-g-PAM MIP tended to be stable after three cycles and maintained 76% of the initial value of the imprinting efficiency even after five repetitions, which was more excellent than that of PAM microsphere. The PP-g-PAM MIP is low cost and easy to be prepared, which would show its potential applications in the fields of extracting and testing required proteins from cells or particulate samples.
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Liver stiffness measurement-based scoring system for significant inflammation related to chronic hepatitis B.
PLoS ONE
PUBLISHED: 01-01-2014
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Liver biopsy is indispensable because liver stiffness measurement alone cannot provide information on intrahepatic inflammation. However, the presence of fibrosis highly correlates with inflammation. We constructed a noninvasive model to determine significant inflammation in chronic hepatitis B patients by using liver stiffness measurement and serum markers.
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Abdominal regional fat distribution on MRI correlates with cholecystolithiasis.
PLoS ONE
PUBLISHED: 01-01-2014
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To determine whether abdominal regional fat distribution pattern on MRI is correlated with cholecystolithiasis.
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A splice mutation and mRNA decay of EXT2 provoke hereditary multiple exostoses.
PLoS ONE
PUBLISHED: 01-01-2014
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Hereditary multiple exostoses (HME) is an autosomal dominant disease. The classical paradigm of mutation screening seeks to relate alterations in the exostosin glycosyltransferase genes, EXT1 and EXT2, which are responsible for over 70% of HME cases. However, the pathological significance of the majority of these mutations is often unclear.
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Mycobacterium tuberculosis Rv3402c enhances mycobacterial survival within macrophages and modulates the host pro-inflammatory cytokines production via NF-kappa B/ERK/p38 signaling.
PLoS ONE
PUBLISHED: 01-01-2014
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Intracellular survival plays a central role in the pathogenesis of Mycobacterium tuberculosis, a process which depends on an array of virulence factors to colonize and replicate within the host. The M. tuberculosis iron regulated open reading frame (ORF) rv3402c, encoding a conserved hypothetical protein, was shown to be up-regulated upon infection in both human and mice macrophages. To explore the function of this ORF, we heterologously expressed the rv3402c gene in the non-pathogenic fast-growing Mycobacterium smegmatis strain, and demonstrated that Rv3402c, a cell envelope-associated protein, was able to enhance the intracellular survival of recombinant M. smegmatis. Enhanced growth was not found to be the result of an increased resistance to intracellular stresses, as growth of the Rv3402c expressing strain was unaffected by iron depletion, H2O2 exposure, or acidic conditions. Colonization of macrophages by M. smegmatis expressing Rv3402c was associated with substantial cell death and significantly greater amount of TNF-? and IL-1? compared with controls. Rv3402c-induced TNF-? and IL-1? production was found to be mediated by NF-?B, ERK and p38 pathway in macrophages. In summary, our study suggests that Rv3402c delivered in a live M. smegmatis vehicle can modify the cytokines profile of macrophage, promote host cell death and enhance the persistence of mycobacterium within host cells.
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The normal transverse mesocolon and involvement of the mesocolon in acute pancreatitis: an MRI study.
PLoS ONE
PUBLISHED: 01-01-2014
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To study the MRI findings of the normal transverse mesocolon and the involvement of the mesocolon in acute pancreatitis (AP) as well as the relationship between the involvement of the mesocolon and the severity of AP.
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Quantitative assessment of the presence and severity of cirrhosis in patients with hepatitis B using right liver lobe volume and spleen size measured at magnetic resonance imaging.
PLoS ONE
PUBLISHED: 01-01-2014
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To determine whether right liver lobe volume (RV) and spleen size measured utilizing magnetic resonance (MR) imaging could identify the presence and severity of cirrhosis in patients with hepatitis B.
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Effect of bacteria on the wound healing behavior of oral epithelial cells.
PLoS ONE
PUBLISHED: 01-01-2014
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Wounded tissue offers opportunity to microflora to adhere, colonize, invade and infect surrounding healthy tissue. The bacteria of the oral cavity have the potential to alter the wound healing process by interacting with keratinocytes. The aim of this study was to investigate mechanisms through which oral bacteria may influence re-epithelialization by interacting with gingival keratinocytes. By an in vitro scratch assay we demonstrate that primary gingival keratinocytes have impaired closure when exposed to two well characterized oral bacteria, P. gingivalis, and to a lesser extent, F. nucleatum. P. gingivalis reduced wound closure by ? 40%, which was partially dependent on proteolytic activity, and bacteria was still present within infected cells 9 days later despite exposure to bacteria for only 24 h. Both oral bacteria caused keratinocyte apoptosis at the wound site with cell death being greatest at the wound edge. P. gingivalis and F. nucleatum adversely affected cell proliferation and the effect also had a spatial component being most striking at the edge. The impact of the bacteria was long lasting even when exposure was brief. Cell migration was compromised in bacteria challenged keratinocytes with P. gingivalis having more severe effect (p<0.05) than F. nucleatum. Quantitative real time PCR of bacteria challenged cells showed that P. gingivalis and to a lesser extent F. nucleatum significantly downregulated cell cycle genes cyclin1, CDK1, and CDK4 (p<0.05) that are critical for GI/S transition. Further, genes associated with cell migration such as integrin beta-3 and -6 were significantly downregulated by P. gingivalis (p<0.05).
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[Effect of the interaction of microorganisms and iron oxides on arsenic releasing into groundwater in Chinese Loess].
Huan Jing Ke Xue
PUBLISHED: 12-25-2013
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A large part of groundwater in the Chinese Loess Plateau area is characterized by high arsenic concentration. Anaerobic bacteria have been considered to play key roles in promoting arsenic releasing from loess to groundwater. However, this hypothesis remains unconfirmed. Based on modeling experiments, this study investigated the speciation of arsenic in loess, and then determined the release rates and quantities of arsenic with the mediation of anaerobic bacteria. The results showed that arsenic contents in loess were between 23 mg.kg-1 and 30 mg.kg-1. No obvious arsenic content difference among loess samples was observed. The ratios for specific adsorbed, iron oxides co-precipitated and silicate co-precipitated arsenic were 37.76% , 36. 15% and 25. 69% , respectively. Indigenous microorganisms, dissimilatory iron reducing bacteria (DIRB) and sulfate reducing bacteria (SRB) could all promote the release of arsenic from loess. Organic matters highly affected the release rates. More than 100 mg.L-1 sodium lactate was required for all bacterial experiments to facilitate obvious arsenic release. Considering the redox condition in loess, the contribution of SRB to arsenic release in loess area was less feasible than that of DIRB and indigenous microorganisms.
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[Evidence-based study of chromate exposed workers health surveillance indexes].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 12-20-2013
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The health surveillance proposal for chromate exposed workers was provided and analyzed on the evidence-based study and then to be improved.
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[Apoptosis of A549 cells induced by cloned Noxa gene].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 12-12-2013
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To clone the Noxa gene and to observe the apoptosis of A549 cells transfected with the recombinant plasmid of pcDNA-Noxa.
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FOXO1 promotes wound healing through the up-regulation of TGF-?1 and prevention of oxidative stress.
J. Cell Biol.
PUBLISHED: 10-21-2013
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Keratinocyte mobilization is a critical aspect of wound re-epithelialization, but the mechanisms that control its precise regulation remain poorly understood. We set out to test the hypothesis that forkhead box O1 (FOXO1) has a negative effect on healing because of its capacity to inhibit proliferation and promote apoptosis. Contrary to expectations, FOXO1 is required for keratinocyte transition to a wound-healing phenotype that involves increased migration and up-regulation of transforming growth factor ?1 (TGF-?1) and its downstream targets, integrin-?3 and -?6 and MMP-3 and -9. Furthermore, we show that FOXO1 functions in keratinocytes to reduce oxidative stress, which is necessary to maintain cell migration and prevent cell death in a TGF-?1-independent manner. Thus, our studies identify a novel function for FOXO1 in coordinating the response of keratinocytes to wounding through up-regulation of TGF-?1 and other factors needed for keratinocyte migration and protection against oxidative stress, which together promote migration and decrease apoptosis.
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[Role of c-Myc in mesenchymal stromal cell-mediated drug resistance in acute leukemia cells].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 10-16-2013
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To explore the role of c-Myc in mesenchymal stromal cell-mediated drug resistance and elucidate the molecular mechanism of acute myeloid leukemia (AML) from the version of tumor microenvironment.
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[Construction and expression of non-structural protein gene 3-4b of HCV 1b based on the adenoassociated virus vector].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
PUBLISHED: 09-19-2013
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OBJECTIVE :To clone 1b type of HCV NS3-4b Gene and express in HEK 293 cells, lay the foundation for further study of the HCV NS3-4b recombinant adeno-associated virus vaccine and its dendritic cell vaccine.
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Diameters of left gastric vein and its originating vein on magnetic resonance imaging in liver cirrhosis patients with hepatitis B: Association with endoscopic grades of esophageal varices.
Hepatol. Res.
PUBLISHED: 08-16-2013
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To determine whether diameters of the left gastric vein (LGV) and its originating vein are associated with endoscopic grades of esophageal varices.
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Synthetic effect between iron oxide and sulfate mineral on the anaerobic transformation of organic substance.
Bioresour. Technol.
PUBLISHED: 08-04-2013
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Synthetic effect between sulfate minerals (gypsum) and iron oxide (hematite) on the anaerobic transformation of organic substance was investigated in the current study. The results showed that gypsum was completely decomposed while hematite was partially reduced. The mineral phase analysis results showed that FeS and CaCO3 was the major mineralization product. Methane generation process was inhibited and inorganic carbon contents in the precipitates were enhanced compared to the control without hematite and gypsum. The inorganic carbon content increased with the increasing of hematite dosages. Co-addition of sulfate minerals and iron oxide would have a potential application prospect in the carbon sequestration area and reduction of the greenhouse gas release. The results would also reveal the role of inorganic mineral in the global carbon cycle.
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Iodine-131 SPET/CT and (18)F-FDG PET/CT for the identification and localization of mediastinal lymph node metastases from differentiated thyroid carcinoma.
Hell J Nucl Med
PUBLISHED: 08-02-2013
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Mediastinal lymph node metastases (MLNM) from differentiated thyroid carcinoma (DTC) are considered difficult to diagnose. The aim of this study was to assess the value of iodine-131 ((131)I) single photon emission tomography/computed tomography (SPET/CT) and of (18)F-fluorodeoxyglucose ((18)F-FDG) positron emission tomography/computed tomography (PET/CT) for the diagnosis of MLNM from DTC. Five hundred and eleven consecutive patients operated for DTC and treated with (131)I for ablation of the remnant thyroid and/or for treatment of metastases were enrolled in the study and underwent an (131)I whole body scan ((131)I-WBS). Thirty seven sites of increased (131)I uptake, on the (131)I-WBS that could be an indication for MLNM were re-evaluated by a (131)I-SPET/CT scan. Thirty four other patients with negative (131)I-WBS but having elevated serum thyroglobulin (Tg), were examined by (18)F-FDG PET/CT to possibly diagnose MLNM. A total of 44 DTC patients with MLNM were identified, among the above 37 and 34 cases: 25/37 (67.6%) cases were examined and identified by (131)I-SPET/CT and 19/34 (55.9%) cases by (18)F-FDG PET/CT. A total of 25 and 19 cases were identified. The male-to-female ratio and the average age in patients with (18)F-FDG-avid MLNM were significantly higher than in patients with (131)I-avid MLNM. Among the above 44 patients, 40 patients had superior mediastinal nodal metastases, 9 had aortic nodal metastases and only 1 inferior mediastinal nodal metastases. A patient could have metastases in more than one site. In conclusion, our study suggests that in 511 operated DTC patients, treated for remnant ablation and/or for metastases and examined by (131)I-WBS, there were 37 cases doubtful of having MLNM in the (131)I-WBS and 34 cases doubtful, because of negative (131)I-WBS and elevated Tg. The (131)I-SPET/CT scan was sensitive for detecting MLNM in 25 of the 37 cases and the (18)F-FDG PET/CT in 19 of the 34 cases. These hybrid imaging modalities, when applied as above, were suitable for detecting more MLNM and thus, better supporting treatment planning in these DTC patients.
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Graves disease in an adolescent with dual congenital ectopia and no orthotopic thyroid gland identified by Tc-99m-pertechnetate SPET/CT imaging.
Hell J Nucl Med
PUBLISHED: 08-02-2013
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This is the first case of Graves disease in an adolescent with lingual and prelaryngeal dual congenital ectopia and no orthotopic thyroid gland identified by technetium-99m-pertechnetate ((99m)TcO(-)4) SPET/CT imaging in a 15 years old boy. After 8 weeks treatment with methimazole, Graves disease subsided. Fine needle aspiration cytology of the mass revealed the normal colloid and normal follicular cells without an atypia or lymphoid elements, suggesting a benign ectopic thyroid gland. In conclusion, there is no report in the literature with DETT lingual and prelaryngeal absence of orthotopic thyroid tissue and Graves disease as in our case. This case also highlights the potential ascendancy of (99m)TcO(-)4 SPET/CT in diagnosing the DETT.
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Beijing ambient particle exposure accelerates atherosclerosis in ApoE knockout mice.
Toxicol. Lett.
PUBLISHED: 07-26-2013
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Air pollution is associated with significant adverse health effects including increased cardiovascular morbidity and mortality. However research on the cardiovascular effect of "real-world" exposure to ambient particulate matter (PM) in susceptible animal model is very limited. In this study, we aimed to investigate the association between Beijing ambient particle exposure and the atherosclerosis development in the apolipoprotein E knockout mice (ApoE(-/-) mice).
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Cardiovascular effects of pulmonary exposure to titanium dioxide nanoparticles in ApoE knockout mice.
J Nanosci Nanotechnol
PUBLISHED: 07-18-2013
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Existing studies on the inhalation toxicology of titanium dioxide (TiO2) have focused on possible carcinogenic capacity; however researches on the cardiovascular effect are limited, particularly in terms of susceptible animal models. The present study examined the inhalation toxicology of nano-TiO2 in ApoE knockout mice (ApoE-/- mice), an atherosclerosis susceptible animal model. The nano-TiO2 particles used were anatase type and the diameter ranged from 5 to 10 nm. ApoE-/- mice were randomly divided into five groups (high dose group, median dose group, low dose group, PBS vehicle control group and the nontreatment control group), each of which were given tracheal instillation of nano-TiO2 at the dose of 100 microg, 50 microg and 10 microg and PBS solution per week respectively, totally for six weeks, while the nontreatment control group received no tracheal instillation. We measured various indicators of inflammation, endothelial dysfunction and lipid metabolism in serum, and determined plaque formation on the aorta. After six weeks of treatment, there was significant difference between the high dose group and PBS control group in terms of C reactive protein (CRP), nitric oxide (NO), endothelial nitric oxide synthases (eNOS), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) in serum. The results also showed ratio of plaque area to luminal area and the ratio of the lipid-rich core area to plaque area in the median and high nano-TiO2 dose group significantly increased respectively in HE stained cross-sections. Our study showed that tracheal instillation of nano-TiO2 particles induced considerable systemic inflammation, endothelial dysfunction and lipid metabolism dysfunction, contributing to the progression of atherosclerosis.
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Alteration of Th1/Th2/Th17 cytokine profile and humoral immune responses associated with chromate exposure.
Occup Environ Med
PUBLISHED: 06-27-2013
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The role of chromate exposure in the deregulation of total lymphocyte and other immune factors is largely unclear.
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[Comparative analysis for the cytotoxicity and genotoxicity of multi-walled carbon nanotubes with different lengths and surface modifications in A549 cells].
Beijing Da Xue Xue Bao
PUBLISHED: 06-19-2013
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To compare the cytotoxicity and DNA strand breakage induced by multi-walled carbon nanotubes (MWCNTs) with different lengths and different surface modifications in human alveolar type II cells (A549 cells).
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Secular changes on the distribution of body mass index among Chinese children and adolescents, 1985-2010.
Biomed. Environ. Sci.
PUBLISHED: 06-18-2013
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To analyze the change in Body Mass Index (BMI) distribution among Chinese children and adolescents for the development of more effective intervention for childhood obesity.
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Splicing biomarkers of disease severity in myotonic dystrophy.
Ann. Neurol.
PUBLISHED: 06-04-2013
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To develop RNA splicing biomarkers of disease severity and therapeutic response in myotonic dystrophy type 1 (DM1) and type 2 (DM2).
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[Construction and virulence evaluation of the virB1-89K gene knockout mutant of type IV-like secretion system of Streptococcus suis serotype 2].
Wei Sheng Wu Xue Bao
PUBLISHED: 05-18-2013
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To construct the virB1-89K gene knockout mutant and its complementary strain of Streptococcus suis serotype 2 (SS2) highly virulent strain 05ZYH33 and evaluate the role of virB1-89K in the pathogenesis of SS2.
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[Effect of natural and hydrothermal synthetic goethite on the release of methane in the anaerobic decomposition process of organic matter].
Huan Jing Ke Xue
PUBLISHED: 05-15-2013
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The effects of natural goethite (NGt) and synthetic goethite (SGt) on the release of methane in the anaerobic biochemical system consisted of dissimilatory iron-reducing bacteria (DIRB) and methane-producing bacteria (MPB) were investigated through batch tests with sodium acetate as the carbon source. To explore the effects and mechanisms of both mineral materials on the release of methane in the anaerobic decomposition process of organic matter in the presence of DIRB, the main gas components and total organic carbon (TOC) , total inorganic carbon (TIC), and Fe2+ in the aqueous phase of the experimental process were determined and XRD analyses were conducted for the solid-phase product. Moreover, the minerals were analyzed by specific surface area (BET), X-ray diffraction (XRD), X-ray fluorescence (XRF). Modified Gompertz equation was used to fit the cumulative methane and carbon dioxide. Results showed that the maximum cumulative production of methane was brought forward by 60-78 days by the addition of goethite and CO2 was effectively reduced by 30% - 67% compared with the control samples. SGt was more effective than NGt in promoting the release of CH4 and reducing the CO, emission. Furthermore, the analysis of the solid product showed that the addition of goethite can fix part of CO2 by the formation of siderite.
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PAHs in the Chinese environment: levels, inventory mass, source and toxic potency assessment.
Environ Sci Process Impacts
PUBLISHED: 05-10-2013
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This paper presents a systematic but preliminary study on the levels, inventory mass, emission sources and risk of exposure to PAHs in China by examining 14 polycyclic aromatic hydrocarbons (from the 16 priority PAHs listed by the U.S. EPA, excluding naphthalene and acenaphthylene) in four main environmental media (air, water, soil and sediment). The concentration of individual PAHs in the air, soil, freshwater, seawater, freshwater sediment and marine sediment of China was compared with the global concentration range (GCR) of PAHs from a large number of studies. The PAH levels were found at the high end of the GCR in the air, at the upper middle of the GCR in the water body, and at the middle of the GCR in the soil and sediment. These indicate that PAHs still are emitted heavily in China. About 530?000 tons of ?14PAH was estimated to be distributed into these four media in China. Soil possesses the highest proportion of the PAHs (60%), and the air has the lowest proportion (<0.5%). Therefore, the soil and sediment play an important role in the storage of PAHs. More than 10 thousand tons of PAHs are emitted from all kinds of sources. Firewood, straw, domestic and coking were considered as the main emissions of PAHs in the energy supply. A benzo[a]pyrene (BaP) based hazard quotient (HQ) was introduced to assess the potential toxic risk of exposure. The terrestrial water environment was found to have a high BaP exposure. The HQ value was more than 1 for 58% of freshwater and 39% of freshwater sediment samples. Urban and developed sites were considered to have high BaP exposure risk.
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Mycobacterium tuberculosis PE_PGRS17 promotes the death of host cell and cytokines secretion via Erk kinase accompanying with enhanced survival of recombinant Mycobacterium smegmatis.
J. Interferon Cytokine Res.
PUBLISHED: 05-10-2013
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Tuberculosis (TB) remains a serious threat to global public health, largely due to the successful manipulation of the host immunity by its etiological agent Mycobacterium tuberculosis. The PE_PGRS protein family of M. tuberculosis might be a contributing factor. To investigate the roles of PE_PGRS17, the gene of PE_PGRS 17 was expressed in nonpathogenic fast growing Mycobacterium smegmatis. We found that the recombinant strain survives better than the control in macrophage cultures, accompanied by more host cell death and a marked higher secretion of tumor necrosis factor-alpha by a recombinant strain compared with control. Blocking the action of Erk kinase by an inhibitor can abolish the above effects. In brief, our data showed that PE_PGRS 17 might facilitate pathogen survival and disserve the host cell via remodeling the macrophages immune niche largely consisting of inflammatory cytokines. This furnishes a novel insight into the immune role of this mycobacterium unique gene family.
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Tumor volume of resectable adenocarcinoma of the esophagogastric junction at multidetector CT: association with regional lymph node metastasis and N stage.
Radiology
PUBLISHED: 05-08-2013
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To determine whether the volume of resectable adenocarcinoma of the esophagogastric junction (AEG) measured at multidetector computed tomography (CT) is associated with regional lymph node metastasis and N stage.
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[Study on the efficacy of quarantine during outbreaks of acute hemorrhagic conjunctivitis outbreaks at schools through the susceptive-infective-quarantine-removal model].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 05-08-2013
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To assess the efficacy of quarantine for acute hemorrhagic conjunctivitis (AHC) outbreaks control in schools, by using the Compartment Model. Through combining the characters of both AHC and compartment model, we built a susceptive-infective-removal (SIR) model suited for AHC outbreaks control in schools, and then quarantine was added into the model to develop a susceptive-infective-quarantine-removal (SIQR) model. An outbreak of AHC in Changsha in 2011 was employed as a sample to assess the effect of quarantine for the prevention and control of AHC. Basic reproduction number (?0) of the AHC outbreak without intervention was 6.80, thus the transmission speed of the disease became quite fast. If no intervention had been adopted, almost all the students, faculties and staff members would have been infected within 23 days, and the accumulative cases would become 738, with the total attack rate (TAR) as 99.73%. The peak of the outbreak was at Sep. 11th and the number of new cases was 126 on that day. The efficacy would have been different if quarantine forces had been taken at different time and differently. The bigger and earlier the quarantine force had been adopted, the lower morbidity peak and the smaller TAR would have been appeared, with better efficacy of outbreak control. If the quarantine rate had been taken at the level of 90% on the sixth day, the accumulative case would have been reduced to 132 and the TAR had become 17.84% consequently. Quarantine program could be used as a main intervention approach to be employed for ACH outbreak at schools.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.