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Find video protocols related to scientific articles indexed in Pubmed.
Genomic organization, transcriptomic analysis, and functional characterization of avian ?- and ?-keratins in diverse feather forms.
Genome Biol Evol
PUBLISHED: 08-24-2014
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Feathers are hallmark avian integument appendages, although they were also present on theropods. They are composed of flexible corneous materials made of ?- and ?-keratins, but their genomic organization and their functional roles in feathers have not been well studied. First, we made an exhaustive search of ?- and ?-keratin genes in the new chicken genome assembly (Galgal4). Then, using transcriptomic analysis, we studied ?- and ?-keratin gene expression patterns in five types of feather epidermis. The expression patterns of ?-keratin genes were different in different feather types, whereas those of ?-keratin genes were less variable. In addition, we obtained extensive ?- and ?-keratin mRNA in situ hybridization data, showing that ?-keratins and ?-keratins are preferentially expressed in different parts of the feather components. Together, our data suggest that feather morphological and structural diversity can largely be attributed to differential combinations of ?- and ?-keratin genes in different intrafeather regions and/or feather types from different body parts. The expression profiles provide new insights into the evolutionary origin and diversification of feathers. Finally, functional analysis using mutant chicken keratin forms based on those found in the human ?-keratin mutation database led to abnormal phenotypes. This demonstrates that the chicken can be a convenient model for studying the molecular biology of human keratin-based diseases.
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Supraomohyoid neck dissection and modified radical neck dissection for clinically node-negative oral squamous cell carcinoma: A prospective study of prognosis, complications and quality of life.
J Craniomaxillofac Surg
PUBLISHED: 08-06-2014
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To assess the prognosis and morbidity between supraomohyoid neck dissection (SOND) and modified radical neck dissection (MRND) for oral squamous cell carcinoma (OSCC) in patients with a clinically node-negative neck (cN0).
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Relationship between Chinese medicine pattern types, clinical severity, and prognosis in patients with acute cerebral infarct.
Explore (NY)
PUBLISHED: 08-03-2013
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We investigated the relationship between Chinese medicine pattern (CMP) types, their severity, and prognosis in patients (n = 187) with acute cerebral infarct (ACI). Six CMPs (wind, phlegm, fire-heat, blood stasis, qi deficiency, and yin deficiency and yang hyperactivity) were evaluated according to inspection, listening and smelling, inquiry, and palpitation. The severity and prognosis of each pattern type was determined according to the Glasgow Coma Scale (GCS), Modified Rankin Scale (MRS), National Institutes of Health Stroke Scale (NIHSS), Barthel Index (BI), and Functional Independence Measure (FIM), recorded at stroke onset and 12 weeks after stroke onset. The phlegm pattern (PP) patients displayed lower GCS, BI, and FIM scales scores, and higher MRS and NIHSS scales scores, than the nonphlegm pattern (N-PP) patients at, and 12 weeks after stroke onset, suggesting the clinical severity is greater and the prognosis is worse in PP patients with ACI than in non-PP patients with ACI.
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Genome-wide patterns of genetic variation in two domestic chickens.
Genome Biol Evol
PUBLISHED: 07-02-2013
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Domestic chickens are excellent models for investigating the genetic basis of phenotypic diversity, as numerous phenotypic changes in physiology, morphology, and behavior in chickens have been artificially selected. Genomic study is required to study genome-wide patterns of DNA variation for dissecting the genetic basis of phenotypic traits. We sequenced the genomes of the Silkie and the Taiwanese native chicken L2 at ?23- and 25-fold average coverage depth, respectively, using Illumina sequencing. The reads were mapped onto the chicken reference genome (including 5.1% Ns) to 92.32% genome coverage for the two breeds. Using a stringent filter, we identified ?7.6 million single-nucleotide polymorphisms (SNPs) and 8,839 copy number variations (CNVs) in the mapped regions; 42% of the SNPs have not found in other chickens before. Among the 68,906 SNPs annotated in the chicken sequence assembly, 27,852 were nonsynonymous SNPs located in 13,537 genes. We also identified hundreds of shared and divergent structural and copy number variants in intronic and intergenic regions and in coding regions in the two breeds. Functional enrichments of identified genetic variants were discussed. Radical nsSNP-containing immunity genes were enriched in the QTL regions associated with some economic traits for both breeds. Moreover, genetic changes involved in selective sweeps were detected. From the selective sweeps identified in our two breeds, several genes associated with growth, appetite, and metabolic regulation were identified. Our study provides a framework for genetic and genomic research of domestic chickens and facilitates the domestic chicken as an avian model for genomic, biomedical, and evolutionary studies.
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Microvascular autologous transplantation of partial submandibular gland for severe keratoconjunctivitis sicca.
Br J Ophthalmol
PUBLISHED: 06-28-2013
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To evaluate the feasibility of microvascular autologous transplantation of partial submandibular gland (SMG) to prevent or reduce epiphora in severe keratoconjunctivitis sicca (KCS).
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Crestal Approach for Maxillary Sinus Augmentation in Patients with ?4?mm of Residual Alveolar Bone.
Clin Implant Dent Relat Res
PUBLISHED: 04-06-2013
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PURPOSE: Less morbidity is the major advantage to a one-stage crestal approach to maxillary sinus elevation. However, the ability to ensure high primary implant stability in a severely atrophied ridge is of chief concern. The purpose of this study is to measure and compare the success rate of implants placed at the time of crestal approach sinus lift in patients with ?4?mm of residual alveolar bone (RAB) and >4?mm of RAB. MATERIALS AND METHODS: In this three-site multicenter study, one hundred two patients, 53 males and 49 females, (23-89 years old; mean?=?56.2) were evaluated. Three experienced surgeons (>15 years) performed the crestal approach sinus lift microsurgeries with simultaneous implant placement. At baseline and at the follow-up appointments, calibrated examiners measured radiographic interproximal bone level using ImageJ for Windows after calibration of the radiographs. References for the bone level measurements were the platform, first and second threads of the implants. Statistical analyses, using STATA version 12, stratified patients according to RAB height (group 1: RAB of ?4?mm; n?=?35 and group 2: RAB?>?4?mm; n?=?67), age, gender, and treatment center. RESULTS: The success rate was 100% for group 1 and 98.51% for group 2 at 6 to 100 months postprosthetic loading (mean?=?29.7 months). The peri-implant bone loss averaged 0.55?mm (interquartile range [IQR]?=?0.5 [0-1]) in group 1 and 0.07?mm (IQR?=?0 [0-0]) in group 2. There was no statistically significant difference between the two groups. Clinical outcomes were independent of age, gender, and treatment center. CONCLUSIONS: The RAB height did not increase crestal bone loss or reduce the success rate of the implants and associated prostheses. The crestal approach should be considered a viable technique for use in patients with residual bone height of ?4?mm and merits further evaluation.
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Extraperitoneal presentation of pseudomyxoma peritonei as retroperitoneal invasion with skin fistula-a case report.
Gan To Kagaku Ryoho
PUBLISHED: 12-29-2011
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Pseudomyxoma peritonei (PMP) is characterized by mucinous ascites and implants throughout the peritoneal cavity. The tumor rarely involves retroperitoneum. We present a patient of PMP with retroperitoneal presentation and spontaneous development of a skin fistula. There had been only 7 such cases reported.
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Instantaneous room-temperature and highly enantioselective ArTi(O-i-Pr)3 additions to aldehydes.
Chem. Commun. (Camb.)
PUBLISHED: 09-27-2011
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Direct asymmetric additions of ArTi(O-i-Pr)(3) to aldehydes catalyzed by a titanium catalyst of (R)-H(8)-BINOL are reported. The reactions proceed instantaneously at room temperature, affording alcohols in ?90% ee. Importantly, the ArTi(O-i-Pr)(3) reagent differentiates the ligand effectiveness in an order of H(8)-BINOL > BINOL > TADDOL > diol 3 > disulfonamide 2.
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[Comparison of lateral arm flap and radial forearm flap in reconstruction after oral cancer ablation].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-09-2011
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To compare the clinical application of lateral arm flap and radial forearm flap in reconstruction after oral cancer ablation.
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Diagnosis and treatment of congenital dilatation of Stensens duct.
Laryngoscope
PUBLISHED: 07-28-2011
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This study aimed to describe the diagnosis and management of congenital dilation of Stensens duct (CDSD) in seven cases.
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[Reconstruction of hypopharyngeal circumferential and cervical esophageal defects with free jejunal interposition in 112 cases].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 07-26-2011
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To investigate the results of reconstruction of hypopharyngeal circumferential and cervical esophageal defects with free jejunal transfer.
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[A preliminary study on free medial sural artery perforator flap for head and neck reconstruction following tumor ablation].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 06-29-2011
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To investigate the application of free medial sural artery perforator flap to reconstruct the defect following head and neck tumor ablation.
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Prevention of radiation-induced xerostomia by submandibular gland transfer.
Head Neck
PUBLISHED: 05-20-2011
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This study was carried out for the purpose of evaluating the efficacy of submandibular gland transfer to prevent radiation-induced xerostomia.
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Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses.
Biotechnol Biofuels
PUBLISHED: 05-07-2011
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Neocallimastix patriciarum is one of the common anaerobic fungi in the digestive tracts of ruminants that can actively digest cellulosic materials, and its cellulases have great potential for hydrolyzing cellulosic feedstocks. Due to the difficulty in culture and lack of a genome database, it is not easy to gain a global understanding of the glycosyl hydrolases (GHs) produced by this anaerobic fungus.
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[Functional maxillary reconstruction with free composite fibula flap].
Beijing Da Xue Xue Bao
PUBLISHED: 02-16-2011
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Maxillary defects resulting from tumor resection or trauma can cause severe functional and cosmetic deformities. Maxillary reconstruction has long been a challenge for oral maxillofacial surgeons. Functional maxillary reconstruction with vascularized composite bone flap and osseointegrated implants is one of the most important improvements in head and neck reconstructive surgery. Since 1999, our research group has performed a comprehensive research on functional maxillary reconstruction with free composite fibula flap. Clinical data of the patients with maxillary reconstruction using free fibula flap were analyzed to describe the indications and principles of perioperative period of this technique. The modified free fibula flexor-hallucis longus myofascial flap was introduced, which could overcome the disadvantages of traditional free composite fibula flap. The donor site morbidity, post-operative speech outcome, mastication function, and quality of life were evaluated objectively. The biomechanical effects of stress distribution on maxilla reconstructed by free fibula composite flap were analyzed by three-dimensional finite element analysis. These studies demonstrated maxillary defects can be reconstructed successfully using free fibula flaps. This procedure also allows dental implant or conventional denture rehabilitation, which can improve the patients appearance and oral function and enhance the overall quality of life. The fibula free flap transfer has a high success rate and low perioperative complication rate, making it an ideal choice for maxillary defect reconstruction.
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Clinical and anatomic study on the ducts of the submandibular and sublingual glands.
J. Oral Maxillofac. Surg.
PUBLISHED: 02-23-2010
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To investigate the relationship between the ducts of the submandibular gland (SMG) and sublingual gland (SLG) and discuss its clinical application relating to SMG radiologic examinations and transfer.
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Primate mandibular reconstruction with prefabricated, vascularized tissue-engineered bone flaps and recombinant human bone morphogenetic protein-2 implanted in situ.
Biomaterials
PUBLISHED: 02-16-2010
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Several studies have validated successful mandibular reconstruction with prefabricated tissue-engineered bone flaps and recombinant human bone morphogenetic protein-2 (rhBMP-2) implanted in situ. Whether rhBMP-2 applied with the prefabrication technique enables faster ossification of mandibular defects than rhBMP-2 applied in situ is unknown. We aimed to compare mandibular reconstruction with prefabricated, vascularized tissue-engineered bone flaps with rhBMP-2 and rhBMP-2 applied in situ in primates (Rhesus monkey). We also compared the use of the carriers demineralized freeze-dried bone allograft (DFDBA) and coralline hydroxyapatite (CHA) for applying rhBMP-2. After computed tomography of the monkey head, custom meshes were made, loaded with rhBMP-2-incorporated DFDBA or CHA, and implanted in the latissimus dorsi muscle. Meanwhile, contralateral segmental mandibular defects were created, and custom meshes loaded with DFDBA, CHA, or rhBMP-2-incooperated DFDBA and CHA were implanted in situ. Thirteen weeks later, the bone flaps with rhBMP-2-incorporated DFDBA or CHA were transferred to repair segmental mandibular defects. The meshes loaded with DFDBA or CHA alone showed no bone regeneration 13 weeks after implantation in latissimus dorsi muscle. Radiography, angiography and histological analysis were used to evaluate the repair and vascularization of the implant. Segmental mandibular defects were successfully restored with prefabricated bone flaps and rhBMP-2-incorporated CHA in situ, but other segmental mandibular defects remained with rhBMP-2-incorporated DFDBA, DFDBA and CHA in situ. Moreover, mandibles reconstructed with rhBMP-2-incorporated CHA bone flaps revealed more regenerated and homogeneous bone formation than did other reconstructions. The study suggested that the prefabrication technique induced better mandibular reconstruction and bone regeneration in quantity and quality.
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[Reliability of venae comitant of facial artery as the donor vein in microvascular autologous submandibular gland transfer].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 07-07-2009
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To analyse the reliability of concomitant venae of facial artery as the donor vein in microvascular autologous submandibular gland transfer.
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[Reliability of superficial temporal artery and vein as the recipient vessels in free flap transfers in head and neck region].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 07-01-2009
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To examine the reliability of superficial temporal artery and vein as the recipient vessels in free flap transfers in head and neck region.
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Applying computer techniques in maxillofacial reconstruction using a fibula flap: a messenger and an evaluation method.
J Craniofac Surg
PUBLISHED: 03-12-2009
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While the application of computer-assisted maxillofacial surgery becomes increasingly popular, the translation from virtual models and surgical plans to actual bedside maneuvers and the evaluation of the repeatability of virtual planning remain to be major challenges. The objective of this study was to experiment the technique of using a resin template as a messenger in maxillofacial reconstruction involving a fibula flap. Another aim was to find a quantitative and objective method to evaluate the repeatability of preoperative planning. Seven patients who underwent maxillary or mandibular reconstruction were included in this study. The mean age was 25 years, and the mean follow-up period was 18.7 months. Virtual planning was carried out before surgery. A resin template was made according to the virtual design of bone graft through rapid prototyping technique and served as a guide when surgeons shaped the fibula flap during surgery. The repeatability of the virtual plan was evaluated based on the matching percentage between the actual postoperative model and the computer-generated outcome. All patients demonstrated satisfactory clinical outcomes. The mean repeatability was 87.5% within 1 mm and 96.5% within 2 mm in isolated bone graft. It was 71.4% within 1 mm and 89.9% within 2 mm in reconstructed mandible or maxilla. These results demonstrated that a resin template based on virtual plan and rapid prototyping technique is a reliable messenger to translate from computer modeling to bedside surgical procedures. The repeatability of a virtual plan can be easily and quantitatively evaluated through our three-dimensional differential analysis method.
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Tangy scent in Toona sinensis (Meliaceae) leaflets: isolation, functional characterization, and regulation of TsTPS1 and TsTPS2, two key terpene synthase genes in the biosynthesis of the scent compound.
Curr Pharm Biotechnol
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Toona sinensis (Chinese Mahogany; Meliaceae), a subtropical deciduous tree, has a tangy scent resembling a mix of shallots and garlic. T. sinensis has long been known for its medicinal efficacy for treating enteritis, dysentery, itch and some cancers. However, its volatile components and their biosynthesis remain unexamined. In this study, we identified the spectrum of volatile compounds, isolated and functionally characterized two terpene synthase genes, Tstps1 and Tstps2, responsible for terpenoid synthesis in T. sinensis leaflets. TsTPS1 and TsTPS2 afford multiple products upon incubation with geranyl and farnesyl diphosphate respectively and mainly regulate the biosynthesis of (+) limonene and ?- elemene in vitro, respectively. Headspace analyses show that 98% of leaflet volatiles were sesquiterpenoids and the developing leaflets released a greater diversity and quantity of volatiles than the mature leaflets did, and that ?-elemene was the dominant component in both of them. These data suggested that tangy scent of T. sinensis consists of a combination of terpenoids and that Tstps2 was the major gene involved in the terpenoid biosynthesis in T. sinensis. In situ hybridization revealed that glandular cells of the leaf rachises accumulated abundant Tstps1 mRNA transcripts. Our GFP-based assay further unprecedentedly demonstrated that the transit-peptide of TsTPS1 targets specifically to the mitochondria.
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[Retrospective study of 93 patients with jaw osteoradionecrosis].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
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To investigate the clinical feature, treatment, and prognosis of hospitalized patients with jaw osteoradionecrosis.
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Polybrominated diphenyl ethers in foodstuffs from Taiwan: level and human dietary exposure assessment.
Sci. Total Environ.
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Polybrominated diphenyl ethers (PBDEs) may contaminate food through bioconcentration and biomagnification. PBDEs often exist in the food chain and are consumed by humans. This study aims to determine the concentrations of PBDEs in food intake and to estimate the daily exposure of Taiwanese citizens to PBDEs. One hundred and eight food samples from nine types of commonly consumed foodstuffs were collected from northern, central, southern, and eastern regions of Taiwan. The samples were analyzed for PBDE level by gas chromatography mass spectrometry. Also, a daily dietary intake survey was conducted of 466 adults (153 men, 313 women) in these four regions of Taiwan. Taiwanese daily dietary intake of PBDE is calculated by means of food PBDEs level and daily dietary intake. The result of this study showed the highest concentration of ?PBDE was found in butter (890.3±309.0 pg/g wet weight), followed by egg and pork (553.0±185.0 pg/g wet weight and 545.4±181.0 pg/g wet weight). Deca-BDE was found the highest concentration among eight kinds PBDEs. The average daily intake of PBDEs for the 466 subjects was 67.95±23.01 ng/day. There was a significant difference between the daily intake of ?PBDE in different regions of Taiwan (p<0.05). The highest daily intake of ?PBDE was in northern Taiwan, which is also the most urbanized area.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.