JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
The Diagnosis of Neonatal Pulmonary Atelectasis Using Lung Ultrasound.
Chest
PUBLISHED: 10-24-2014
Show Abstract
Hide Abstract
Generally?the diagnosis of neonatal pulmonary atelectasis (NPA) is based on history, clinical and chest x-ray (CXR) findings while ultrasound could not be used in lung disease diagnostics. Recently, ultrasound has been used for the diagnosis of many kinds lung conditions, but few studies have investigated ultrasound for the diagnosis of NPA. In this study, we evaluated the usefulness of lung ultrasound for the diagnosis of NPA.
Related JoVE Video
Insight into the reactivity and electronic structure of dinuclear dinitrosyl iron complexes.
Inorg Chem
PUBLISHED: 10-03-2014
Show Abstract
Hide Abstract
A combination of N/S/Fe K-edge X-ray absorption spectroscopy (XAS), X-ray diffraction data, and density functional theory (DFT) calculations provides an efficient way to unambiguously delineate the electronic structures and bonding characters of Fe-S, N-O, and Fe-N bonds among the direduced-form Roussin's red ester (RRE) [Fe2(?-SPh)2(NO)4](2-)(1) with {Fe(NO)2}(10)-{Fe(NO)2}(10) core, the reduced-form RRE [Fe2(?-SPh)2(NO)4](-)(3) with {Fe(NO)2}(9)-{Fe(NO)2}(10) core, and RRE [Fe2(?-SPh)2(NO)4] (4) with {Fe(NO)2}(9)-{Fe(NO)2}(9) core. The major contributions of highest occupied molecular orbital (HOMO) 113?/? in complex 1 is related to the antibonding character between Fe(d) and Fe(d), Fe(d), and S atoms, and bonding character between Fe(d) and NO(?*). The effective nuclear charge (Zeff) of Fe site can be increased by removing electrons from HOMO to shorten the distances of Fe···Fe and Fe-S from 1 to 3 to 4 or, in contrast, to increase the Fe-N bond lengths from 1 to 3 to 4. The higher IR ?NO stretching frequencies (1761, 1720 cm(-1) (4), 1680, 1665 cm(-1) (3), and 1646, 1611, 1603 cm(-1) (1)) associated with the higher transition energy of N1s ??*(NO) (412.6 eV (4), 412.3 eV (3), and 412.2 eV (1)) and the higher Zeff of Fe derived from the transition energy of Fe1s ? Fe3d (7113.8 eV (4), 7113.5 eV (3), and 7113.3 eV (1)) indicate that the N-O bond distances of these complexes are in the order of 1 > 3 > 4. The N/S/Fe K-edge XAS spectra as well as DFT computations reveal the reduction of complex 4 yielding complex 3 occurs at Fe, S, and NO; in contrast, reduction mainly occurs at Fe site from complex 3 to complex 1.
Related JoVE Video
Synthesis, self-assembly and photophysical properties of oligo(2,5-dihexyloxy-1,4-phenylene vinylene)-block-poly(ethylene glycol).
Soft Matter
PUBLISHED: 10-03-2014
Show Abstract
Hide Abstract
We describe the synthesis and characterization of a family of diblock copolymers with 5 units of a dihexyloxy-phenylenevinylene block (OHPV) connected to a series of poly(ethylene glycol) (PEG) chains of different average lengths (12, 45 and 115 PEG units: OHPV5-b-PEG12, OHPV5-b-PEG45, OHPV5-b-PEG115). All three polymers underwent self-assembly in ethanol, a good solvent for the PEG units, but poor for the OHPV segment. The nature of the structures formed depends sensitively on the length of the PEG block. OHPV5-b-PEG115 formed long fiber-like micelles of uniform width, whereas OHPV5-b-PEG45 formed fragile broad ribbons. We also obtained thin ribbons with OHPV5-b-PEG12 but they tend to fold and twist upon themselves. The structures obtained were characterized by transmission electron microscopy (TEM) and atomic force microscopy (AFM), as well as by wide-angle X-ray scattering (WAXS) and differential scanning calorimetry (DSC). In addition, their photophysical properties were examined by UV-vis, steady state fluorescence and fluorescence decay measurements. The results of these experiments indicate that the OHPV groups pack differently in the fiber-like micelles of OHPV5-b-PEG115 than in the lamellar structures formed by OHPV5-b-PEG45.
Related JoVE Video
[Rapid quantitative analysis of hydrocarbon composition of furfural extract oils using attenuated total reflection infrared spectroscopy].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-02-2014
Show Abstract
Hide Abstract
A set of rapid analysis system for hydrocarbon composition of heavy oils was designed using attenuated total reflection FTIR spectrometer and chemometrics to determine the hydrocarbon composition of furfural extract oils. Sixty two extract oil samples were collected and their saturates and aromatics content data were determined according to the standard NB/SH/T0509-2010, then the total contents of resins plus asphaltenes were calculated by the subtraction method in the percentage of weight. Based on the partial least squares (PLS), calibration models for saturates, aromatics, and resin+asphaltene contents were established using attenuated total reflection FTIR spectroscopy, with their SEC, 1.43%, 0.91% and 1.61%, SEP, 1.56%, 1.24% and 1.81%, respectively, meeting the accuracy and repeatability required for the standard. Compared to the present standard method, the efficiency of hydrocarbon composition analysis for furfural extract oils is significantly improved by the new method which is rapid and simple. The system could also be used for other heavy oil analysis, with excellent extension and application foreground.
Related JoVE Video
Design and Synthesis of a New Layered Thermoelectric Material LaPbBiS3O.
Inorg Chem
PUBLISHED: 10-01-2014
Show Abstract
Hide Abstract
A new quinary oxysulfide LaPbBiS3O was designed and successfully synthesized via a solid-state reaction in a sealed evacuated quartz tube. This material, composed of stacked NaCl-like [M4S6] (where M = Pb, Bi) layers and fluorite-type [La2O2] layers, crystallizes in the tetragonal space group P4/nmm with a = 4.0982(1) Å, c = 19.7754(6) Å, and Z = 2. Electrical resistivity and Hall effect measurements demonstrate that it is a narrow gap semiconductor with an activation energy of ?17 meV. The thermopower and the figure of merit at room temperature were measured to be -52 ?V/K and 0.23, respectively, which makes LaPbBiS3O and its derivatives be promising for thermoelectric applications.
Related JoVE Video
Parkinson's disease patients with pain suffer from more severe non-motor symptoms.
Neurol. Sci.
PUBLISHED: 09-06-2014
Show Abstract
Hide Abstract
Non-motor symptoms, including pain, depression, sleep disorder, and olfactory dysfunction, occur frequently in patients with Parkinson's disease (PD), even before the onset of motor symptoms. Although studies have examined the correlation between pain and depression or sleep disorder in PD, few studies have investigated the correlation between pain and a range of other non-motor symptoms of PD. PD patients (n = 142) with or without pain were included in the study. PD severity was evaluated with the Unified Parkinson's Disease Rating Scale (UPDRS) and the Hoehn and Yahr (H/Y) staging scale. Pain severity was analyzed with the Visual Analog Scale. The Hamilton Rating Scale for Depression (HRSD; 24 items), Montreal Cognitive Assessment Beijing Version (MoCA), and non-motor questionnaire (NMSQT) measured symptoms of depression, cognitive function, and non-motor symptoms. The incidence of pain was 47.9 % in patients with PD, most of whom had moderate pain levels. Patients with pain showed higher HRSD, UPDRS, H/Y, and NMSQT scores and lower MoCA scores compared to those of patients without pain. HRSD and NMSQT scores were closely related with pain (P < 0.001). Non-motor symptoms were more prominent in patients with pain compared to that of controls and PD patients without pain.
Related JoVE Video
Arterial Hemorrhage from Cesarean Scar: A rare cause of Recurring Massive Uterine Bleeding and Successful Surgical Management.
J Minim Invasive Gynecol
PUBLISHED: 08-25-2014
Show Abstract
Hide Abstract
Abnormal uterine bleeding and other gynecologic complications associated with previous cesarean section scar are only recently being identified and described. Herein we report a rare case of a woman with recurring massive uterine bleeding after two cesarean sections. Curettage and hormone therapy were unsuccessfully used in an attempt to control the bleeding. After she was transferred to our hospital she had another episode of vaginal bleeding which was successfully managed with oxytocin and hemostatic. Diagnostic hysteroscopy performed under anesthesia revealed an abnormal transected artery in the cesarean section scar with a thrombus visible. In the treatment at the beginning of Laparoscopic Management we adopted temporary bilateral uterine artery occlusion with titanium clips to prevent massive hemorrhage. Secondly with the aid of hysteroscopy, the bleeding site was opened and then the cesarean scar was wedge-resected and stitched interruptedly with 1-0 absorbable sutures. The postoperative recovery was uneventful. It would seem that the worldwide use of cesarean section delivery may contribute to the risk of gynecologic disturbances including some unrecognized and complex conditions as seen in this case.
Related JoVE Video
Improved outcomes of transported neonates in Beijing: the impact of strategic changes in perinatal and regional neonatal transport network services.
World J Pediatr
PUBLISHED: 08-15-2014
Show Abstract
Hide Abstract
Infants born outside perinatal centers may have compromised outcomes due to the transfer speed and efficiency to an appropriate tertiary center. This study aimed to evaluate the impact of regional coordinated changes in perinatal supports and retrieval services on the outcome of transported neonates in Beijing, China.
Related JoVE Video
Scratch wound closure of myoblasts and myotubes is reduced by inflammatory mediators.
Int Wound J
PUBLISHED: 08-14-2014
Show Abstract
Hide Abstract
Complex interactions exist between muscle repair processes and acute inflammatory responses that are initiated by exercise-induced muscle damage. The purpose of this study was to examine whether inflammatory mediators secreted by activated macrophages affect the migration of myogenic cells to the injury site. Migration was measured using a scratch wound closure assay in C2 C12 -derived myogenic cells incubated in activated macrophage-conditioned medium. Both myoblast and myotube migrations were significantly reduced in activated macrophage-conditioned medium compared with control medium. Furthermore, we demonstrated that the inhibitory effect on myoblast and myotube migrations was mediated, at least in part, by the two major cytokines secreted by activated macrophages, tumour necrosis factor (TNF)-? and interleukin (IL)-6. These findings suggest that the migration rate of myogenic cells may be reduced by inflammatory mediators. It may provide useful insights for future researches on the role of macrophages in the process of muscle repair and regeneration.
Related JoVE Video
A convenient tandem one-pot synthesis of donor-acceptor-type triphenylene 2,3-dicarboxylic esters from diarylacetylene.
Org. Biomol. Chem.
PUBLISHED: 08-09-2014
Show Abstract
Hide Abstract
A tandem one-pot method for the direct synthesis of polysubstituted triphenylene 2,3-dicarboxylic esters with different substitution patterns was developed by enyne metathesis of diarylacetylene, followed by Diels-Alder, aromatization and a cyclization cascade.
Related JoVE Video
Low mannose-binding lectin (MBL) levels and MBL genetic polymorphisms associated with the risk of neonatal sepsis: An updated meta-analysis.
Early Hum. Dev.
PUBLISHED: 08-07-2014
Show Abstract
Hide Abstract
Relatively low serum mannose-binding lectin (MBL) levels and MBL genetic polymorphisms have been implicated as high risk factors for neonatal sepsis. However, different studies have reported conflicting findings and have generally been underpowered to exclude modest effect sizes.
Related JoVE Video
Ethyleneglycol tungsten complexes of calix[6 and 8]arenes: synthesis, characterization and ROP of ?-caprolactone.
Dalton Trans
PUBLISHED: 08-07-2014
Show Abstract
Hide Abstract
By varying the reaction conditions, the reaction of [W(eg)3] (eg = 1,2-ethanediolato) with p-tert-butylcalix[n]areneHn (n = 6 or 8) in refluxing toluene affords, following work-up, a number of products which have been fully characterized. From the reaction of p-tert-butylcalix[6]areneH6 with one or two equivalents of [W(eg)3], only the oxo-bridged complex {[W(eg)]2(?-O)p-tert-butylcalix[6]arene} (1) could be isolated, whereas the use of four equivalents of [W(eg)3], in the presence of molecular sieves, afforded {[W(eg)2]2p-tert-butylcalix[6]areneH2}·2MeCN (2); molecules of 2 pack in bi-layers. Under similar conditions, use of one or two equivalents of [W(eg)3] and p-tert-butylcalix[8]areneH8 afforded {[W(eg)]2p-tert-butylcalix[8]arene}·MeCN (3) in which each tungsten centre was bound by four calixarene oxygens. By contrast, the small orange prisms resulting from the use of four equivalents of [W(eg)3] and p-tert-butylcalix[8]areneH8 were shown by synchrotron radiation to be a mixture of two isomers 4a/4b·3.5MeCN). In the major isomer {1,2-[W(eg)2]2p-tert-butylcalix[8]areneH4} (4a), two tungsten centres bind to neighbouring sets of phenolate oxygens, whereas in the minor isomer {1,3-[W(eg)2]2p-tert-butylcalix[8]areneH4} (4b), there is a protonated phenolic group between the two pairs of phenolate oxygens bound to tungsten; the major?:?minor ratio is about 83?:?17. Use of p-tert-butyltetrahomodioxacalix[6]areneH6 with two equivalents of [W(eg)3] resulted in the isolation of {[WO(eg)]2p-tert-butyltetrahomodioxacalix[6]areneH2} (5·0.83toluene·MeCN), in which each dimethyleneoxa bridge is bound to an oxotungsten(vi) centre. Complexes 1-5, together with the known complex [W(eg)p-tert-butylcalix[4]arene] (6), have been screened for their ability to ring open polymerize (ROP) ?-caprolactone; for 1, 2 and 5, 6 conversion rates were good (>88%) at 110 °C over 12 or 24 h, whereas the calix[8]arene complexes 3 and 4 under the same conditions were inactive.
Related JoVE Video
[Research progress in using near infrared for monitoring drug quality in domestic circulation].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 08-07-2014
Show Abstract
Hide Abstract
Drugs are special goods that are directly related to public health, so their quality should be monitored in any link of their whole lifecycle. With nondestructive, rapid and environmentally friendly characteristics, near infrared technique is highly suitable for monitoring drug quality in the open market as well as the distribution channels. The present paper reviewed the current situation (analytical objects, methods and instruments) about the application of near infrared spectroscopy in monitoring the quality of the final drug products, Chinese crude drug or decoction pieces in domestic circulation since 1997, expounded the unsolved problems and future prospects.
Related JoVE Video
[Chemical constituents from twigs of Piper hancei].
Zhong Yao Cai
PUBLISHED: 08-06-2014
Show Abstract
Hide Abstract
To investigate the chemical constituents from the twigs of Piper hancei.
Related JoVE Video
[The role of CD4+CD25+ regulatory T cells in helminth infection immunity].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 07-29-2014
Show Abstract
Hide Abstract
Regulatory T cells (Tregs), a class of CD4+ T lymphocytes which are different from the Th1 and Th2 lymphocytes, have immunosuppressive function and play an important role in maintaining immune homeostasis and immune tolerance. Previous studies showed that the generation of Tregs could be induced, and Tregs reduced the immune pathological damage to the host by inhibiting T-cell function in helminth infection.
Related JoVE Video
Sleep disorders increase the risk of burning mouth syndrome: a retrospective population-based cohort study.
Sleep Med.
PUBLISHED: 07-23-2014
Show Abstract
Hide Abstract
Sleep disorders (SD), including apnea and nonapnea, and burning mouth syndrome (BMS) have been mutually associated with systemic diseases. Based on our research, the association between BMS and SD has not been elucidated. We determined whether SD patients have an increased risk of BMS.
Related JoVE Video
[An optimal selection method of samples of calibration set and validation set for spectral multivariate analysis].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 07-11-2014
Show Abstract
Hide Abstract
The side effects in spectral multivariate modeling caused by the uneven distribution of sample numbers in the region of the calibration set and validation set were analyzed, and the "average" phenomenon that samples with small property values are predicted with larger values, and those with large property values are predicted with less values in spectral multivariate calibration is showed in this paper. Considering the distribution feature of spectral space and property space simultaneously, a new method of optimal sample selection named Rank-KS is proposed. Rank-KS aims at improving the uniformity of calibration set and validation set. Y-space was divided into some regions uniformly, samples of calibration set and validation set were extracted by Kennard-Stone (KS) and Random-Select (RS) algorithm respectively in every region, so the calibration set was distributed evenly and had a strong presentation. The proposed method were applied to the prediction of dimethylcarbonate (DMC) content in gasoline with infrared spectra and dimethylsulfoxide in its aqueous solution with near infrared spectra. The "average" phenomenon showed in the prediction of multiple linear regression (MLR) model of dimethylsulfoxide was weakened effectively by Rank-KS. For comparison, the MLR models and PLS1 models of MDC and dimethylsulfoxide were constructed by using RS, KS, Rank-Select, sample set partitioning based on joint X- and Y-blocks (SPXY) and proposed Rank-KS algorithms to select the calibration set, respectively. Application results verified that the best prediction was achieved by using Rank-KS. Especially, for the distribution of sample set with more in the middle and less on the boundaries, or none in the local, prediction of the model constructed by calibration set selected using Rank-KS can be improved obviously.
Related JoVE Video
Identification and characterization of the antifungal substances of a novel Streptomyces cavourensis NA4.
J. Microbiol. Biotechnol.
PUBLISHED: 07-09-2014
Show Abstract
Hide Abstract
A new actinomycete strain NA4 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antifungal activities against soil-borne fungal pathogens. It was identified as Streptomyces cavourensis by morphological, physiological and phylogenetic analysis based on 16S rRNA gene sequence. The main antifungal components were isolated and identified from the fermentation culture as bafilomycins B1 and C1. These compounds exhibited significant antifungal activities and broad antifungal spectrum. The results suggest that the Streptomyces cavourensis NA4 and bafilomycins B1 and C1 could be used as potential biocontrol agents for soil-borne fungal diseases of plants.
Related JoVE Video
Endosonographic features of histologically proven gastric ectopic pancreas.
Gastroenterol Res Pract
PUBLISHED: 06-03-2014
Show Abstract
Hide Abstract
Gastric ectopic pancreas is an uncommon developmental anomaly and its histological diagnosis is usually difficult by using a conventional biopsy forceps. In the literature, most cases of gastric ectopic pancreas were usually diagnosed by gross pattern during endoscopic examination or features of endoscopic ultrasound. In contrast, this disease was seldom diagnosed by histology in clinical practice. Although the typical endoscopic ultrasonographic features of ectopic pancreas include heterogeneous echogenicity, indistinct borders, and a location within 2 or more layers, it can also exhibit hypoechoic homogeneous echogenicity and a distinct border within the fourth sonographic layer (muscularis propria) similar to the endoscopic ultrasonographic features of gastrointestinal stromal tumors. In our study, we found that 53% of gastric ectopic pancreas originated within the fourth sonographic layer, demonstrating hypoechoic, homogeneous echogenicity, and distinct borders. Therefore, recognizing endoscopic ultrasonographic features, combining with deep biopsy, endoscopic ultrasound-guided fine needle aspiration/core needle biopsy can prevent conducting unnecessary resection. Surgical resection is the mainstay treatment for symptomatic gastric ectopic pancreas, but endoscopic resection using endoscopic mucosal resection or endoscopic submucosal dissection technique provides an alternative method of removing superficial-type and deep-type gastric ectopic pancreas.
Related JoVE Video
[Effects of recombinant human interleukin-11 on LPS-induced intestinal epithelial cell injury in rats].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 05-27-2014
Show Abstract
Hide Abstract
To study the effects of recombinant human interleukin-11 (rhIL-11) on the proliferation and apoptosis of rat intestinal epithelial cell line (IEC-6).
Related JoVE Video
Isolation, identification and characterization of related substances in furbenicillin.
J. Antibiot.
PUBLISHED: 05-21-2014
Show Abstract
Hide Abstract
Furbenicillin is a broad-spectrum semisynthetic penicillin with strong antibacterial activity against Gram-negative bacteria. In this study, three impurities in furbenicillin, including an unknown epimer, were determined. On the basis of a complete analysis of the spectrum (MS, (1)H,(13)C, 2D NMR and CD) and the results of chemical methods, the unknown epimer impurity was identified as 10-epi-furbenicillin (impurity 1). Isolation and structure elucidation of impurity 1 was also reported here for the first time.The Journal of Antibiotics advance online publication, 22 October 2014; doi:10.1038/ja.2014.145.
Related JoVE Video
Lung ultrasonography for the diagnosis of severe neonatal pneumonia.
Chest
PUBLISHED: 05-17-2014
Show Abstract
Hide Abstract
Lung ultrasonography is useful for the diagnosis of pneumonia in children and adults. This study investigated the lung ultrasound findings in severe neonatal pneumonia.
Related JoVE Video
Clinical, microbiologic, and outcome analysis of mycotic aortic aneurysm: the role of endovascular repair.
Surg Infect (Larchmt)
PUBLISHED: 05-06-2014
Show Abstract
Hide Abstract
Mycotic aortic aneurysm (MAA) is an infrequent but devastating form of vascular disease.
Related JoVE Video
A MCP-1 promoter polymorphism at G-2518A is associated with spontaneous preterm birth.
Mol. Genet. Genomics
PUBLISHED: 04-25-2014
Show Abstract
Hide Abstract
Monocyte chemoattractant protein-1 (MCP-1) is an important chemokine involved in the pathogenesis of spontaneous preterm birth (SPTB). We examined whether the MCP-1 G-2518A polymorphism is associated with the risk of SPTB in a Chinese population. The MCP-1 G-2518A polymorphism was genotyped in 569 preterm singleton neonates and in 673 term neonates using polymerase chain reaction-restriction fragment length polymorphism analysis. The distribution of the MCP-1 G-2518A genotype and the allele frequencies between the SPTB patients and the controls were not significantly different in the overall sample. However, we found that the AA genotype was associated with significantly increased susceptibility to very SPTB (<32 weeks) [odds ratio (OR) 2.07; 95 % confidence interval (CI), 1.27-3.36; P = 0.005) and extremely SPTB (<28 weeks) (OR 2.74; 95 % CI, 1.10-6.72; P = 0.014) compared with -2518G-positive genotypes (GG + GA genotypes). When extremely preterm neonates and very preterm neonates were combined, the AA genotype was also significantly associated with increased susceptibility to SPTB (OR 2.23; 95 % CI, 1.40-3.54; P < 0.001). The MCP-1 G-2518A polymorphism was not associated with increased susceptibility to SPTB in patients with premature rupture of the membranes (PROM) or in those without PROM. Our findings suggest that the MCP-1 G-2518A polymorphism may plays a role in mediating the susceptibility to SPTB in the Chinese population. Knowledge of genetic factors contributing to the pathogenesis of SPTB may have implications for screening and treatment of this disorder.
Related JoVE Video
[Perinatal high-risk factors for necrotizing enterocolitis in preterm infants: a case-control study].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 04-23-2014
Show Abstract
Hide Abstract
To study the timing of presentation and perinatal high-risk factors for necrotizing enterocolitis (NEC) in preterm infants with a gestational age of <33 weeks.
Related JoVE Video
A novel silent deletion, an insertion mutation and a nonsense mutation in the TCOF1 gene found in two Chinese cases of Treacher Collins syndrome.
Mol. Genet. Genomics
PUBLISHED: 04-13-2014
Show Abstract
Hide Abstract
Treacher Collins syndrome (TCS) is the most common and well-known craniofacial disorder caused by mutations in the genes involved in pre-rRNA transcription, which include the TCOF1 gene. This study explored the role of TCOF1 mutations in Chinese patients with TCS. Mutational analysis of the TCOF1 gene was performed in three patients using polymerase chain reaction and direct sequencing. Among these three patients, two additional TCOF1 variations, a novel 18 bp deletion and a novel 1 bp insertion mutation, were found in patient 1, together with a novel nonsense mutation (p.Ser476X) and a previously reported 4 bp deletion (c.1872_1875delTGAG) in other patients. Pedigree analysis allowed for prediction of the character of the mutation, which was either pathological or not. The 18 bp deletion of six amino acids, Ser-Asp-Ser-Glu-Glu-Glu (798*803), which was located in the CKII phosphorylation site of treacle, seemed relatively benign for TCS. By contrast, another novel mutation of c.1072_1073insC (p.Gln358ProfsX23) was a frameshift mutation and expected to result in a premature stop codon. This study provides insights into the functional domain of treacle and illustrates the importance of clinical and family TCS screening for the interpretation of novel sequence alterations.
Related JoVE Video
Periodontal disease with treatment reduces subsequent cancer risks.
QJM
PUBLISHED: 04-10-2014
Show Abstract
Hide Abstract
The aim of our study was to evaluate the relationship between routine treatment of periodontal disease (PD) and the subsequent risks for cancers in Taiwan.
Related JoVE Video
Sleep disorders increase the risk of osteoporosis: a nationwide population-based cohort study.
Sleep Med.
PUBLISHED: 03-22-2014
Show Abstract
Hide Abstract
This study evaluated the relationship between sleep disorders (SDs) and osteoporosis risk in Taiwan.
Related JoVE Video
High maternal serum estradiol environment in the first trimester is associated with the increased risk of small-for-gestational-age birth.
J. Clin. Endocrinol. Metab.
PUBLISHED: 02-28-2014
Show Abstract
Hide Abstract
There are increasing concerns that a disrupted endocrine environment may disturb the growth of the fetus. Assisted reproductive technology (ART) situates gamete/embryo in a supraphysiological estradiol (E2) environment and, thus, provides an ideal model to investigate this problem.
Related JoVE Video
Acute kidney injury in adults receiving extracorporeal membrane oxygenation.
J. Formos. Med. Assoc.
PUBLISHED: 02-17-2014
Show Abstract
Hide Abstract
Extracorporeal membrane oxygenation (ECMO) has been utilized for critically ill patients such as patients with postcardiotomy cardiogenic shock or life-threatening respiratory failure. Acute kidney injury (AKI) that develops during ECMO is associated with a very poor outcome, possibly because of accumulated extravascular water causing interstitial overload, impaired oxygen transport through tissues, and increased extravascular lung water volume with impaired O2 transport. Increased water is associated with subsequent organ dysfunction, particularly of the heart, lungs, and brain. Based on single-center studies, the incidence of AKI is 70-85% in ECMO patients. Therefore, renal replacement therapy is required in approximately 50% of these patients. This review summarizes three modalities that can be used to introduce renal replacement therapy to patients on ECMO, the pathophysiology of AKI in ECMO, and the impact of AKI on mortality. This review also identifies specific research-focused questions that need to be addressed to predict AKI early and to improve outcomes in this at-risk adult population.
Related JoVE Video
Modified high-intensity interval training increases peak cardiac power output in patients with heart failure.
Eur. J. Appl. Physiol.
PUBLISHED: 02-15-2014
Show Abstract
Hide Abstract
Although high-intensity interval aerobic training (HIT) effectively improves aerobic fitness, the risk of cardiac arrest transiently increases during strenuous physical exertion in patients with cardiovascular disease. For safety and efficacy concerns, this investigation explored the effect of a modified HIT (mHIT) on exertional ventilatory-hemodynamic efficiency in heart failure patients with reduced ejection fraction (HFREF).
Related JoVE Video
Inhibiting mast cell degranulation by HO-1 affects dendritic cell maturation in vitro.
Inflamm. Res.
PUBLISHED: 02-12-2014
Show Abstract
Hide Abstract
Mast cell (MC) degranulation can break peripheral immune tolerance. However, its mechanism remains unclear. Our goal was to study the stabilization of MC membranes by heme oxygenase-1 (HO-1) in order to influence dendritic cell (DC) function.
Related JoVE Video
Immunoprotection against influenza virus H9N2 by the oral administration of recombinant Lactobacillus plantarumNC8 expressing hemagglutinin in BALB/c mice.
Virology
PUBLISHED: 01-27-2014
Show Abstract
Hide Abstract
The H9N2 avian influenza virus (AIV) has become increasingly concerning due to its role in severe economic losses in the poultry industry. Transmission of AIV to mammals, including pigs and humans, has accelerated efforts to devise preventive strategies. To develop an effective oral vaccine against H9N2 AIV, a recombinant Lactobacillus plantarum NC8 strain expressing the hemagglutinin (HA) gene of H9N2 AIV was constructed in this study. Mice were orally immunized with the recombinant NC8-pSIP409-HA strain, and sIgA, IgG and HI antibodies were produced by the NC8-pSIP409-HA strain, which also induced CD8(+) T cell immune responses. Most importantly, oral administration produced complete protection against challenge with mouse-adapted H9N2 virus. These results indicate that the recombinant NC8-pSIP409-HA was more effective at inducing the mucosal, humoral and cellular immune responses. Therefore, L. plantarum NC8-pSIP409-HA could become a promising oral vaccine candidate against H9N2 AIV.
Related JoVE Video
Prognosis of patients with acute respiratory distress syndrome on extracorporeal membrane oxygenation: the impact of urine output on mortality.
Ann. Thorac. Surg.
PUBLISHED: 01-27-2014
Show Abstract
Hide Abstract
Extracorporeal membrane oxygenation (ECMO) has been utilized for patients in critical condition, including life-threatening respiratory failure and postcardiotomy cardiogenic shock. This study analyzed the outcomes of patients with acute respiratory distress syndrome (ARDS) treated by ECMO and identified the relationship between prognosis and urine output (UO) obtained on the first day of ECMO support.
Related JoVE Video
Protective immunity conferred by porcine circovirus 2 ORF2-based DNA vaccine in mice.
Microbiol. Immunol.
PUBLISHED: 01-25-2014
Show Abstract
Hide Abstract
Post-weaning multisystemic wasting syndrome (PMWS) associated with porcine circovirus type 2 (PCV2) has caused the swine industry significant health challenges and economic damage. Although inactivated and subunit vaccines against PMWS have been used widely, so far no DNA vaccine is available. In this study, with the aim of exploring a new route for developing a vaccine against PCV2, the immunogenicity of a DNA vaccine was evaluated in mice. The pEGFP-N1 vector was used to construct a PCV2 Cap gene recombinant vaccine. To assess the immunogenicity of pEGFP-Cap, 80 BALB/c mice were immunized three times at 2 weekly intervals with pEGFP-Cap, LG-strain vaccine, pEGFP-N1 vector or PBS and then challenged with PCV2. IgG and cytokines were assessed by indirect ELISA and ELISA, respectively. Specimens stained with hematoxylin and eosin (HE) and immunohistochemistry (IHC) techniques were examined histopathologically. It was found that vaccination of the mice with the pEGFP-Cap induced solid protection against PCV2 infection through induction of highly specific serum IgG antibodies and cytokines (IFN-? and IL-10), and a small PCV2 viral load. The mice treated with the pEGFP-Cap and LG-strain developed no histopathologically detectable lesions (HE stain) and IHC techniques revealed only a few positive cells. Thus, this study demonstrated that recombinant pEGFP-Cap substantially alleviates PCV2 infection in mice and provides evidence that a DNA vaccine could be an alternative to PCV2 vaccines against PMWS.
Related JoVE Video
Neonatal outcomes of very preterm infants from a neonatal intensive care center.
World J Pediatr
PUBLISHED: 01-25-2014
Show Abstract
Hide Abstract
Information about clinical outcomes of very preterm (VPT) infants in tertiary neonatal intensive care unit (NICU) setting is scant in China. This study aimed to investigate the mortality and morbidity of VPT infants admitted to BaYi Children's Hospital, which serves as a NICU referral center for the city of Beijing, China.
Related JoVE Video
Schisandrin A and B affect subventricular zone neurogenesis in mouse.
Eur. J. Pharmacol.
PUBLISHED: 01-22-2014
Show Abstract
Hide Abstract
Schisandrin A and B (Sch A and B) are the main effective components extracted from the oriental medicine Schisandra chinensis which is traditionally used to enhance mental and intellectual function. Although their neuroprotective effects have been demonstrated, their influences on neurogenesis are still unknown. In the brain, new neural cells born in the subventricular zone (SVZ) next to the lateral ventricles migrate along the rostral migratory stream (RMS) to the olfactory bulb (OB). To investigate the effects of Sch A and B on neurogenesis in the SVZ-RMS-OB system, Sch A and B were intragastrically administrated at dosages of 1, 10 and 20 mg/kg d respectively. The dose of 10 mg/kg d was selected for further analysis based on the preliminary analysis. In the SVZ, significant increases of phosphohistone H3 positive proliferating cells and the intensity of glial fibrillary acidic protein (GFAP+) cells were noticed in Sch B group. In the RMS, Sch A treatment augmented the intensity of doublecortin positive neuroblasts. In the OB, Sch A decreased tyrosine hydroxylase cells and Calbindin (CalB+) cells, while Sch B increased CalB+ cells and Calretinin (CalR+) cells. These results suggest that Sch B stimulates SVZ proliferation by enhancing GFAP+ cells and improves the survival of OB interneurons, while Sch A promotes neuroblast formation in the RMS but impairs the survival of OB interneurons. The present study provides the first evidence that Sch B exerts neuroprotective functions by enhancing neurogenesis, but Sch A mainly negatively regulates neurogenesis, in the adult SVZ-RMS-OB system.
Related JoVE Video
Superconductivity in a layered Ta4Pd3Te16 with PdTe2 chains.
J. Am. Chem. Soc.
PUBLISHED: 01-15-2014
Show Abstract
Hide Abstract
Superconductivity in low-dimensional compounds has long attracted much interest. Here we report superconductivity in a low-dimensional ternary telluride Ta4Pd3Te16 in which the repeating layers contain edge-sharing octahedrally coordinated PdTe2 chains along the crystallographic b axis. Measurements of electrical resistivity, magnetic susceptibility and specific heat on the Ta4Pd3Te16 crystals, grown via a self-flux method, consistently demonstrate bulk superconductivity at 4.6 K. Further analyses of the data indicate significant electron-electron interaction, which allows electronic Cooper pairing in the present system.
Related JoVE Video
Therapeutic opinion on endovascular repair for mycotic aortic aneurysm.
Ann Vasc Surg
PUBLISHED: 01-06-2014
Show Abstract
Hide Abstract
Our aim in this study was to assess the feasibility of endovascular repair for mycotic aortic aneurysms (MAAs) and to provide a therapeutic opinion.
Related JoVE Video
Role of mtDNA Haplogroups in the Prevalence of Osteoarthritis in Different Geographic Populations: A Meta-Analysis.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
Osteoarthritis (OA) is the most common form of arthritis and has become an increasingly important public-health problem. However, the pathogenesis of OA is still unclear. In recent years, its correlation with mtDNA haplogroups attracts much attention. We aimed to perform a meta-analysis to investigate the association between mtDNA haplogroups and OA.
Related JoVE Video
Microsatellite polymorphism in the heme oxygenase-1 gene promoter and the risk of atrial fibrillation in Taiwanese.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
Atrial fibrillation (AF) is associated with increased oxidative stress. Emerging evidence suggests that heme oxygenase-1 (HO-1) is a potent antioxidant system against various oxidative stress-related diseases. The human HO-1 promoter has a GT-repeat length polymorphism that can determine the level of gene transcription.
Related JoVE Video
Value of amplitude-integrated electroencephalograph in early diagnosis and prognosis prediction of neonatal hypoxic-ischemic encephalopathy.
Int J Clin Exp Med
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
To investigate value of amplitude-integrated electroencephalograph (aEEG) in early diagnosis and prediction of long-term prognosis of neonatal hypoxic-ischemic encephalopathy (HIE), 120 HIE Children were randomly assigned into aEEG group and control group (n = 60 per group). Children in each group were sub-divided into mild, moderate and severe HIE groups (n = 20 per group). 1, 3, 14 and 28 days after birth, aEEG was performed in aEEG group; 3, 14 and 28 days after birth, neonatal behavioral neurological assessment (NBNA) was done in both groups. Children who discharged were followed up at adjusted gestational age of 12 months with Denver Developmental Screening Test (DDST) and prognosis evaluation.
Related JoVE Video
[Lung ultrasound for diagnosis of neonatal atelectasis].
Zhonghua Er Ke Za Zhi
PUBLISHED: 12-17-2013
Show Abstract
Hide Abstract
The diagnosis of neonatal atelectasis (NA) is usually based on clinical manifestations and chest X-rays, lung ultrasounds are not included in the diagnostic work-up of NA.Recently, ultrasounds have been used extensively and successfully in the diagnosis of many kinds of lung diseases, but few studies have addressed NA. The aim of this study was to evaluate the ultrasound imaging features of NA-and to evaluate the value of lung ultrasound in diagnosing NA.
Related JoVE Video
Variable range hopping conductivity and spin glass behavior in spin-ladder Ba0.6K0.4Fe2Se3 single crystals.
J Phys Condens Matter
PUBLISHED: 12-09-2013
Show Abstract
Hide Abstract
Ba0.6K0.4Fe2Se3 (BKFS) single crystals were investigated by means of measurements of powder x-ray diffraction, temperature-dependent resistivity, anisotropic dc magnetization, ac magnetic susceptibility and specific heat. The powder x-ray diffraction indicates staggered iron displacements along the ladders with short and long Fe-Fe bond lengths (2.64(2) and 2.91(2) ?) variation. The resistivity of BKFS exhibits variable range hopping behavior with ln(?) ? T(-1/2) at low temperature. The magnetic susceptibility ?(T) exhibits a sharp cusp at around 20 K in a zero-field-cooled process. The frequency-dependent ac magnetic susceptibility reveals that the cusp feature is attributable to spin glass behavior. The anisotropic ac magnetic susceptibility indicates that BKFS is probably an anisotropic Heisenberg-like spin glass with its easy magnetization plane perpendicular to the chain direction. The specific heat also supports an insulating and spin glass ground state. Extended Curie-Weiss behavior above 40 K was observed with a reduced effective moment (?eff = 1.66 ?B/Fe for H?b and ?eff = 1.82 ?B/Fe for H ? b) in BKFS, which is close to the spin-only magnetism with [Formula: see text].
Related JoVE Video
Retrospective review of vancomycin-induced nephrotoxicity in patients with leukemia.
J Oncol Pharm Pract
PUBLISHED: 10-31-2013
Show Abstract
Hide Abstract
BackgroundThe occurrence of nephrotoxicity with vancomycin is approximately 17%, but can increase to 35% when combined with other nephrotoxic agents. Patients with hematologic malignancies may be at greater risk for vancomycin-induced nephrotoxicity due to nephrotoxic chemotherapy and tumor lysis syndrome.
Related JoVE Video
Synthesis of benzimidazoles by potassium tert-butoxide-promoted intermolecular cyclization reaction of 2-iodoanilines with nitriles.
Org. Biomol. Chem.
PUBLISHED: 09-30-2013
Show Abstract
Hide Abstract
The synthesis of benzimidazoles by intermolecular cyclization reaction of 2-iodoanilines with nitriles has been developed. These reactions proceeded without the aid of any transition metals or ligands and just using KOBu(t) as the base. A variety of substituted benzimidazole derivatives can be synthesized by the approach.
Related JoVE Video
Non-apnea Sleep Disorder Increases the Risk of Periodontal Disease: A Retrospective Population-Based Cohort Study (Version 3).
J. Periodontol.
PUBLISHED: 09-24-2013
Show Abstract
Hide Abstract
Aim: The aim of this study was to determine whether non-apnea sleep disorder patients with comorbidity have an increased risk of periodontal disease. Materials and Methods: Patients newly diagnosed with non-apnea sleep disorders (NA-SD) in 1997-2010 were identified as the study cohort from the Taiwan National Health Insurance (TNHI) database. For each NA-SD patient, two matched controls without sleep disorders were randomly selected for comparison. Results: The overall incidence rate ratio of severe periodontal diseases was 39% higher in the NA-SD cohort than in the without NA-SD cohort (7.93 vs. 5.69 per 1,000 person-years), with an adjusted HR of 1.36, (95% CIs = 1.30-1.43). The effect of NA-SD on the risk of severe periodontal diseases was higher in young patients, and middle-aged patients, as compared with patients over 65 years of age (? 35 years of age, HR = 1.13, 95% CIs = 1.04-1.24; 35-50 years of age, HR = 1.73, 95% CIs = 1.61-1.86; 50- 65 years of age, HR =1.69, 95% CI = 1.58-1.81). Conclusion: Non-apnea sleep disorder might increase the risk of periodontal disease.
Related JoVE Video
[Research progress in cytokines and signaling pathways for promoting pulmonary angiogenesis and vascular development].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 09-17-2013
Show Abstract
Hide Abstract
With the advances in pre- and post-natal medical care, the incidence of bronchopulmonary dysplasia (BPD) is on the rise, while its pathogenesis remains not clear. New BPD theory shows that the core pathogenesis of BPD is simple alveolar structure and pulmonary microvascular abnormalities that eventually lead to reduced pulmonary gas exchange, so the research on pulmonary microvascular development was gradually taken seriously. Pulmonary angiogenesis and vascular development require the participation of various cytokines and signaling pathways, the most important of which include VEGF/VEGFR pathway, Ang/Tie pathway, Ephrins/Eph pathway, and Notch/Jagged1 pathway. These cytokines and signaling pathways play important roles in pulmonary vascular development.
Related JoVE Video
Development of chitosan/heparin nanoparticle-encapsulated cytolethal distending toxin for gastric cancer therapy.
Nanomedicine (Lond)
PUBLISHED: 09-11-2013
Show Abstract
Hide Abstract
Aim: The aim of this work was to develop pH-responsive nanoparticles encapsulating CdtB and to demonstrate that these particles represent a potential therapeutic agent for gastric cancer. Materials & methods: Chitosan/heparin nanoparticle-encapsulated CdtB was prepared and the delivery efficiency was monitored by confocal laser scanning microscopy. The molecular basis of the nanoparticle-encapsulated CdtB-mediated p53 activation pathway was explored by immunoblot analysis. Antitumoral activities were investigated by analyzing the cell cycle and apoptosis. Results: Chitosan/heparin nanoparticle-encapsulated CdtB preferentially inhibited the proliferation of cells derived from gastric cancer, but not in primary gastric epithelial cells. Treatment of cells with nanoparticle-encapsulated CdtB enhanced cell-cycle arrest at G2/M, followed by apoptosis. Moreover, our data showed that the mechanism for nanoparticle-encapsulated CdtB-induced cell death was mediated by ATM-dependent DNA damage checkpoint responses. Conclusion: These findings indicate that chitosan/heparin nanoparticle-encapsulated CdtB could represent a new CdtB delivery strategy for the treatment of gastric cancer. Original submitted 8 May 2012; Revised submitted 5 February 2013.
Related JoVE Video
Self-assembly of amphiphilic homopolymers bearing ferrocene and carboxyl functionalities: effect of polymer concentration, ?-cyclodextrin, and length of alkyl linker.
Langmuir
PUBLISHED: 08-12-2013
Show Abstract
Hide Abstract
Three new acrylamide monomers containing ferrocene and tert-butyl ester groups were first synthesized via multistep nucleophilic substitution reaction under mild conditions followed by reversible addition-fragmentation chain transfer (RAFT) homopolymerization to give well-defined homopolymers with narrow molecular weight distributions (M(w)/M(n) ? 1.36). The target amphiphilic homopolymers were obtained by the acidic hydrolysis of tert-butyoxycarbonyls to carboxyls in every repeating unit using CF3COOH. The self-assembly behaviors of these amphiphilic homopolymers bearing both ferrocene and carboxyl moieties in each repeating unit in aqueous media were investigated by transmission emission microscopy (TEM), dynamic light scattering (DLS), and atomic force microscopy (AFM). Large compound micelles with different morphologies were formed by these amphiphilic homopolymers, which consist of the corona formed by hydrophilic carboxyls and the core containing numerous reverse micelles with hydrophilic islands of carboxyls in continuous hydrophobic phase of ferrocene-based segments. The morphologies of the formed micelles could be tuned by the concentration of amphiphilic homopolymers, pH value of the solution, the length of -CH2 linker between ferrocene group and carboxyl, and the amount of ?-cyclodextrin (?-CD).
Related JoVE Video
A radiologists guide to treatment response criteria in oncologic imaging: functional, molecular, and disease-specific imaging biomarkers.
AJR Am J Roentgenol
PUBLISHED: 07-26-2013
Show Abstract
Hide Abstract
This article reviews the functional, molecular, and disease-specific imaging biomarkers of treatment response.
Related JoVE Video
A radiologists guide to treatment response criteria in oncologic imaging: anatomic imaging biomarkers.
AJR Am J Roentgenol
PUBLISHED: 07-26-2013
Show Abstract
Hide Abstract
The purpose of this article is to describe the imaging biomarkers of treatment response and provide an overview of anatomic imaging biomarkers.
Related JoVE Video
Differential diagnosis of colorectal polyps with respect to similar capabilities of white light colonoscopy and narrow-band imaging.
Int J Colorectal Dis
PUBLISHED: 07-23-2013
Show Abstract
Hide Abstract
The responses of polyps to light essentially determine the diagnostic capability of an endoscopy system in differentiating adenomas from hyperplastic polyps. Compared with white light colonoscopy (WLC), narrow-band imaging (NBI) is expected to improve the diagnostic capability. The diagnostic capabilities of WLC and NBI are evaluated and compared based on the polyp responses.
Related JoVE Video
Lipopolysaccharides shapes the human Whartons jelly-derived mesenchymal stem cells in vitro.
Cell. Physiol. Biochem.
PUBLISHED: 07-21-2013
Show Abstract
Hide Abstract
Although the expression of toll-like receptors (TLRs) on different types of human mesenchymal stem cells (hMSCs) has recently been reported, controversy remains regarding the presence of TLR4 as well as its engagement and impact on human Whartons jelly-derived MSCs (hWJ-MSCs).
Related JoVE Video
Identification of a mutation in GDF9 as a novel cause of diminished ovarian reserve in young women.
Hum. Reprod.
PUBLISHED: 07-12-2013
Show Abstract
Hide Abstract
Do any mutations in growth differentiation factor 9 (GDF9) have a role in diminished ovarian reserve (DOR) in young women?
Related JoVE Video
Decreased number of Gemini of coiled bodies and U12 snRNA level in amyotrophic lateral sclerosis.
Hum. Mol. Genet.
PUBLISHED: 06-04-2013
Show Abstract
Hide Abstract
Disappearance of TAR-DNA-binding protein 43 kDa (TDP-43) from the nucleus contributes to the pathogenesis of amyotrophic lateral sclerosis (ALS), but the nuclear function of TDP-43 is not yet fully understood. TDP-43 associates with nuclear bodies including Gemini of coiled bodies (GEMs). GEMs contribute to the biogenesis of uridine-rich small nuclear RNA (U snRNA), a component of splicing machinery. The number of GEMs and a subset of U snRNAs decrease in spinal muscular atrophy, a lower motor neuron disease, suggesting that alteration of U snRNAs may also underlie the molecular pathogenesis of ALS. Here, we investigated the number of GEMs and U11/12-type small nuclear ribonucleoproteins (snRNP) by immunohistochemistry and the level of U snRNAs using real-time quantitative RT-PCR in ALS tissues. GEMs decreased in both TDP-43-depleted HeLa cells and spinal motor neurons in ALS patients. Levels of several U snRNAs decreased in TDP-43-depleted SH-SY5Y and U87-MG cells. The level of U12 snRNA was decreased in tissues affected by ALS (spinal cord, motor cortex and thalamus) but not in tissues unaffected by ALS (cerebellum, kidney and muscle). Immunohistochemical analysis revealed the decrease in U11/12-type snRNP in spinal motor neurons of ALS patients. These findings suggest that loss of TDP-43 function decreases the number of GEMs, which is followed by a disturbance of pre-mRNA splicing by the U11/U12 spliceosome in tissues affected by ALS.
Related JoVE Video
[Therapeutic effect of Ommaya reservoir implantation on hydrocephalus in premature infants following intraventricular hemorrhage and factors associted with the therapeutic effect].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 05-17-2013
Show Abstract
Hide Abstract
To observe the therapeutic effect of Ommaya reservoir implantation on hydrocephalus in premature infants following intraventricular hemorrhage (IVH) and to investigate factors influencing the therapeutic effect.
Related JoVE Video
Surgical treatment for patients with periodontal disease reduces risk of end-stage renal disease: a nationwide population-based retrospective cohort study.
J. Periodontol.
PUBLISHED: 05-07-2013
Show Abstract
Hide Abstract
Background: The association between periodontal disease treatment and end-stage renal disease (ESRD) remains unclear. This study aims to determine whether surgical periodontal treatment reduces ESRD risk. Methods: From the insurance claims data of patients with periodontal disease who were free of ESRD from 1997 to 2009, 35,496 patients were identified who underwent surgery for subgingival curettage and/or periodontal flap and are considered the treatment cohort. For comparison, 141,824 patients who did not undergo these treatments were considered the no-treatment cohort. Follow-ups were performed until the end of 2009 to estimate the incidence and risk of ESRD in these two cohorts. Cox proportional hazard regression was used to estimate the related hazard ratio (HR) and 95% confidence interval (CI) of ESRD. Results: The incidence of ESRD was lower in the treatment cohort than in the no-treatment cohort (4.66 versus 7.38 per 10,000 person-years), with an adjusted HR of 0.59 (95% CI = 0.46 to 0.75). Sex- and age-specific analysis showed that the incidence rate ratio of the treatment cohort to the no-treatment cohort was higher for women than for men and declined with age. The risks of ESRD were consistently lower in the treatment cohort even when compared by comorbidity. Conclusions: Patients with periodontal disease who undergo procedures for subgingival curettage and/or periodontal flap have a remarkably decreased risk of ESRD.
Related JoVE Video
[Determination of alpha-cellulose content of natural cellulose pulp in a new clean pulping process using near infrared diffuse reflectance spectroscopy].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 04-17-2013
Show Abstract
Hide Abstract
A new near infrared diffuse reflectance spectroscopy method is proposed to rapidly detect alpha-cellulose content of natural cellulose (plant fiber: cotton, wood) pulp in a new clean pulping process. One hundred forty two samples were collected and their alpha-cellulose content data were determined by standard method GB/T 9107-1999. The samples were homogenized by grinding pretreatment to improve spectroscopy measurement accuracy. Effective classification models were built by SIMCA, with the total correct identification. Using partial least squares (PLS) quantitative calibration, alpha-cellulose of the whole and separate cotton and wood pulp was established, with the correlation coefficients of 0.954, 0.911, 0.839, SEP, 0.024, 0.012 and 0.016, respectively. The repeatability results obtained by the new method are in agreement with the results from GB/T 9107-1999. The new method is feasible for determining alpha-cellulose content of natural cellulose (plant fiber: cotton, wood) in clean pulping process.
Related JoVE Video
High-performance anion-exchange chromatography coupled with diode array detection for the determination of dencichine in Panax notoginseng and related species.
J Sep Sci
PUBLISHED: 03-28-2013
Show Abstract
Hide Abstract
A high-performance anion-exchange chromatography coupled with diode array detection method was developed for the determination of dencichine in Panax notoginseng and related species. The analysis was performed on an Eprogen Synchropak WAX column (4.6 × 250 mm, 6 ?m) with 50 mM NaH2 PO4 aqueous solution isocratic elution. The method was validated in terms of linearity, sensitivity, precision, stability, and accuracy. It was found that the calibration curve for dencichine showed good linearity (R(2) = 0.9999) within the test range. The LOD and LOQ were 0.77 and 3.06 ng, respectively. The RSD for intra- and interday repeatability was 0.2 and 0.5%, respectively. The test solution of dencichine is stable at least for three days at room temperature and for seven days at 4 °C. The mean recovery of dencichine was 102.0%. The established method was successfully applied to determine dencichine in the raw root of P. nogoginseng, P. ginseng, and P. quinquefolium as well as the steamed root of P. notoginseng. Compared with previous reports, this method is sensitive, selective, and accurate, which is helpful to evaluate the quality of P. notoginseng and related species.
Related JoVE Video
[Bedside diode laser photocoagulation for 103 cases with serious retinopathy of prematurity in NICU].
Zhonghua Er Ke Za Zhi
PUBLISHED: 03-27-2013
Show Abstract
Hide Abstract
To evaluate the efficacy and safety of the bedside diode laser photocoagulation for severe retinopathy of prematurity in neonatal intensive care unit (NICU).
Related JoVE Video
Simultaneous determination of flavonoids, isochlorogenic acids and triterpenoids in Ilex hainanensis Using high performance liquid chromatography coupled with diode array and evaporative light scattering detection.
Molecules
PUBLISHED: 01-14-2013
Show Abstract
Hide Abstract
A high performance liquid chromatography coupled with diode array and evaporative light scattering detection (HPLC-DAD-ELSD) method for simultaneous determination of eight major bioactive compounds including two flavonoids (rutin and eriodictyol-7-O-?-D-glucopyranoside), two isochlorogenic acids (isochlorogenic acid A and isochlorogenic acid C) and four triterpenoids (ilexhainanoside D, ilexsaponin A1, ilexgenin A and ursolic acid) in Ilex hainanensis has been developed for the first time. The 283 nm wavelength was chosen for determination of two flavonoids and two isochlorogenic acids. ELSD was applied to determine four triterpenoids. The analysis was performed on an Agilent Zorbax SB-C18 column (250 × 4.6 mm i.d., 5 µm) with gradient elution of 0.2% formic acid in water and acetonitrile. The method was validated for linearity, limit of detection, limit of quantification, precision, repeatability and accuracy. The proposed method has been successfully applied for simultaneous quantification of the analytes in four samples of Ilex hainanensis, which is helpful for quality control of this plant.
Related JoVE Video
Inhibition of Helicobacter pylori CagA-Induced Pathogenesis by Methylantcinate B from Antrodia camphorata.
Evid Based Complement Alternat Med
PUBLISHED: 01-08-2013
Show Abstract
Hide Abstract
The bacterial pathogen Helicobacter pylori (Hp) is the leading risk factor for the development of gastric cancer. Hp virulence factor, cytotoxin-associated gene A (CagA) interacted with cholesterol-enriched microdomains and leads to induction of inflammation in gastric epithelial cells (AGS). In this study, we identified a triterpenoid methylantcinate B (MAB) from the medicinal mushroom Antrodia camphorata which inhibited the translocation and phosphorylation of CagA and caused a reduction in hummingbird phenotype in HP-infected AGS cells. Additionally, MAB suppressed the Hp-induced inflammatory response by attenuation of NF-?B activation, translocation of p65 NF-?B, and phosphorylation of I?B-?, indicating that MAB modulates CagA-mediated signaling pathway. Additionally, MAB also suppressed the IL-8 luciferase activity and its secretion in HP-infected AGS cells. On the other hand, molecular structure simulations revealed that MAB interacts with CagA similarly to that of cholesterol. Moreover, binding of cholesterol to the immobilized CagA was inhibited by increased levels of MAB. Our results demonstrate that MAB is the first natural triterpenoid which competes with cholesterol bound to CagA leading to attenuation of Hp-induced pathogenesis of epithelial cells. Thus, this study indicates that MAB may have a scope to develop as a therapeutic candidate against Hp CagA-induced inflammation.
Related JoVE Video
Using extracorporeal membrane oxygenation to rescue acute myocardial infarction with cardiopulmonary collapse: the impact of early coronary revascularization.
Resuscitation
PUBLISHED: 01-07-2013
Show Abstract
Hide Abstract
To investigate the therapeutic impact of combining extracorporeal membrane oxygenation (ECMO) and early coronary revascularization on acute myocardial infarction (AMI)-induced cardiopulmonary collapse.
Related JoVE Video
The SEPS1 G-105A polymorphism is associated with risk of spontaneous preterm birth in a Chinese population.
PLoS ONE
PUBLISHED: 01-01-2013
Show Abstract
Hide Abstract
Inflammation plays an important role in the etiology and pathophysiology of spontaneous preterm birth (SPTB), and selenoprotein S (SEPS1) is involved in regulating the inflammatory response. Recently the G-105A promoter polymorphism in SEPS1 was shown to increase pro-inflammatory cytokine expression. We examined whether this functional polymorphism was related to the risk of SPTB in a Chinese population. We also examined the impact of premature rupture of membranes (PROM) on susceptibility to SPTB. The SEPS1 G-105A polymorphism was genotyped in 569 preterm singleton neonates and 673 term neonates by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. ? (2) tests and logistic regression analyses were used to calculate the odds ratios (ORs) and 95% confidence intervals (95% CIs). We observed that, compared with the GG genotype, -105A positive genotypes (GA + AA genotypes) were associated with significantly increased susceptibility to SPTB (adjusted OR, 1.87; 95% CI, 1.36-2.57; P<0.001). The -105A positive genotypes were also significantly associated with increased susceptibility to SPTB, both in the patients with PROM (adjusted OR, 2.65; 95% CI, 1.73-4.03; P<0.001) and in those without PROM (adjusted OR, 1.56; 95% CI, 1.09-2.24; P?=?0.015). The -105A positive genotypes were also significantly associated with increased susceptibility to SPTB between extremely preterm neonates and controls (adjusted OR, 4.46; 95% CI, 1.86-10.73; P?=?0.002) and between moderately preterm neonates and controls (adjusted OR, 1.76; 95% CI, 1.25-2.47; P?=?0.001). Our findings suggest that the SEPS1 G-105A polymorphism contributes to the risk of developing SPTB in a Chinese population.
Related JoVE Video
Characterization of putative cholesterol recognition/interaction amino acid consensus-like motif of Campylobacter jejuni cytolethal distending toxin C.
PLoS ONE
PUBLISHED: 01-01-2013
Show Abstract
Hide Abstract
Cytolethal distending toxin (CDT) produced by Campylobacter jejuni comprises a heterotrimeric complex formed by CdtA, CdtB, and CdtC. Among these toxin subunits, CdtA and CdtC function as essential proteins that mediate toxin binding to cytoplasmic membranes followed by delivery of CdtB into the nucleus. The binding of CdtA/CdtC to the cell surface is mediated by cholesterol, a major component in lipid rafts. Although the putative cholesterol recognition/interaction amino acid consensus (CRAC) domain of CDT has been reported from several bacterial pathogens, the protein regions contributing to CDT binding to cholesterol in C. jejuni remain unclear. Here, we selected a potential CRAC-like region present in the CdtC from C. jejuni for analysis. Molecular modeling showed that the predicted functional domain had the shape of a hydrophobic groove, facilitating cholesterol localization to this domain. Mutation of a tyrosine residue in the CRAC-like region decreased direct binding of CdtC to cholesterol rather than toxin intermolecular interactions and led to impaired CDT intoxication. These results provide a molecular link between C. jejuni CdtC and membrane-lipid rafts through the CRAC-like region, which contributes to toxin recognition and interaction with cholesterol.
Related JoVE Video
Prognosis of patients on extracorporeal membrane oxygenation plus continuous arteriovenous hemofiltration.
Chang Gung Med J
PUBLISHED: 12-27-2011
Show Abstract
Hide Abstract
Extracorporeal membrane oxygenation (ECMO) has been utilized for critically ill patients such as those with life-threatening respiratory failure or post-cardiotomy cardiogenic shock. Patients on ECMO with acute renal failure have high mortality rates. This study identifies specific predictors of hospital mortality for patients receiving ECMO and continuous arteriovenous hemofiltration (CAVH).
Related JoVE Video
Adult bicuspid aortic valve endocarditis with extensive paravalvular invasion attributable to disseminated varicella zoster infection.
Ann Thorac Cardiovasc Surg
PUBLISHED: 12-09-2011
Show Abstract
Hide Abstract
We report a previously healthy 21-year-old man who developed disseminated varicella zoster infection complicated with encephalitis, acute renal insufficiency, liver dysfunction, and an apparent pustular skin superinfection with Staphylococcus aureus. He later developed an extensively destructive endocarditis affecting a congenital bicuspid aortic valve, accompanied with leaflet perforation, complete atrio-ventricular (AV) block, and invasion of vegetation to both left and right atrium; the endocarditis was attributed to the same skin pathogen, S. aureus. He underwent radical debridement of the aortic valve, membranous ventricular septum, and mitral anterior fibrous trigone, followed by reconstruction of intracardiac defects with 2 autologous pericardial patches and aortic valve replacement. After a permanent pacemaker implantation and 4 weeks of antibiotic treatment, he was discharged after an uneventful postoperative course.
Related JoVE Video
One stone two birds: construction of polysubstituted benzenes from the same starting material and precatalyst by switching the active sites of catalyst with different additives.
Chem. Commun. (Camb.)
PUBLISHED: 11-23-2011
Show Abstract
Hide Abstract
Two different tetrasubstituted benzenes were selectively constructed from the same starting materials by tuning the active sites of the Grubbs second generation catalyst (GC-II) with CuI or AgOTf as the additive.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.