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Find video protocols related to scientific articles indexed in Pubmed.
Proteomic Investigation of Signatures for Geniposide-Induced Hepatotoxicity.
J. Proteome Res.
PUBLISHED: 10-23-2014
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Evaluating the safety of traditional medicinal herbs and their major active constituents is critical for their widespread usage. Geniposide, a major active constituent with a defined structure from the traditional medicinal herb Gardenia jasminoides ELLIS fruit, exhibits remarkable anti-inflammatory, antiapoptotic, and antifibrotic properties and has been used in a variety of medical fields, mainly for the treatment of liver diseases. However, geniposide-induced hepatotoxicity and methods for the early detection of hepatotoxicity have yet to be reported. In this study, geniposide-induced hepatotoxicity was investigated. In addition, candidate biomarkers for the earlier detection of geniposide-induced hepatotoxicity were identified using a label-free quantitative proteomics approach on a geniposide overdose-induced liver injury in a rat model. Using an accurate intensity-based, absolute quantification (iBAQ)-based, one-step discovery and verification approach, a candidate biomarker panel was easily obtained from individual samples in response to different conditions. To determine the biomarkers' early detection abilities, five candidate biomarkers were selected and tested using enzyme-linked immunosorbent assays (ELISAs). Two biomarkers, glycine N-methyltransferase (GNMT) and glycogen phosphorylase (PYGL), were found to indicate hepatic injuries significantly earlier than the current gold standard liver biomarker. This study provides a first insight into geniposide-induced hepatotoxicity in a rat model and describes a method for the earlier detection of this hepatotoxicity, facilitating the efficient monitoring of drug-induced hepatotoxicity.
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Effects of Hypothermia on S100B and Glial Fibrillary Acidic Protein in Asphyxia Rats After Cardiopulmonary Resuscitation.
Cell Biochem. Biophys.
PUBLISHED: 09-12-2014
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The aim of the study was to investigate the effects of hypothermia on S100B and glial fibrillary acidic protein (GFAP) in serum and hippocampus CA1 area in asphyxiated rats after cardiopulmonary resuscitation (CPR). A total of 100 SD rats were designated into four groups: group A, sham operation group; group B, rats received conventional resuscitation; group C, rats received conventional resuscitation and hypothermia at cardiac arrest; group D, rats received conventional resuscitation and hypothermia at 30 min after restoration of spontaneous circulation (ROSC). Rats were then killed by cardiac arrest at 2 and 4 h after ROSC; brain tissue was taken to observe dynamic changes of S100B and GFAP in serum and hippocampus CA1 area. Following ROSC, S100B levels increased from 2 to 4 h in group B, C, and D. In addition, S100B in serum and hippocampus CA1 area was all significantly increased at different time points compared with group A (P < 0.05). Following ROSC, serum S100B level at 2 h in group C was significantly decreased compared with group B, but the difference was not statistically significant (P > 0.05). Moreover, S100B in serum at 4 h after ROSC was significantly decreased (P < 0.05), S100B in cortex was significantly decreased (P < 0.05). The expression of GFAP was also examined. GFAP level in hippocampus CA1 area was significantly decreased in group B, C, and D at 4 h after ROSC compared with group A (P < 0.05). S100B and GFAP were expressed in rat serum and hippocampus CA2 area at early stage after ROSC, which can be used as sensitive markers for brain injury diagnosis and prognosis prediction. Hypothermia is also shown to reduce brain injury after CPR.
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[Material and mechanisms for evaluation of Shuanghuanglian injection induced pseudoanaphylactoid reactions].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 10-25-2011
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To investigate the substance basis and the mechanism of pseudoanaphylactoid reactions (PR) induced by Shuanghuanglian injection (SHLI).
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Synergistic effect of CH-296 and interferon gamma on cytokine-induced killer cells expansion for patients with advanced-stage malignant solid tumors.
Cancer Biother. Radiopharm.
PUBLISHED: 07-28-2011
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Cytokine-induced killer cells (CIKs) are heterogenous antitumor effectors including interferon gamma (IFN-?)-amplified CD3(+)CD56(+) cells. CH-296 has been shown to stimulate T-cell proliferation in the presence of T cell receptor (TCR)-stimulating signals. The purpose of this study was to investigate the synergistic effect of CH-296 and IFN-? on expansion of CIKs for treating patients with advanced-stage malignant solid tumors.
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[Evaluation of vitro hepatotoxicity of monocrotaline by precision-cut liver slice technique].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 06-11-2011
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To modify the empirical method of precision-cut liver slice technique, and study the hepatotoxicity of monocrotaline by this technique.
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[Development of animal model for anaphylactoid test of rodent].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 05-24-2011
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To establish a simple and feasible method of anaphylactoid test on awaked small animals for screening and assessing anaphylactoid reaction of traditional Chinese medicine (TCM) injection with different concentration of tween 80.
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[Review on experiment of traditional Chinese medicine treating to osteoporosis].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 05-24-2011
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Searched the articles between 2000 and 2010, found out and summarized the articles with the topic on the experiment and new techniques of traditional Chinese medicine treating to osteoporosis. The preventive and therapeutic effect to osteoporosis by traditional Chinese medicine had been developed in the past 10 years. The study on standardization of experimental drugs, and the mechanism study with modern cell culture techniques should be enhanced.
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Melanoma B16-F1 cells coated with fusion protein of mouse calreticulin and virus G-protein coupled receptor induced the antitumor immune response in Balb/C mice.
Cancer Biol. Ther.
PUBLISHED: 03-15-2011
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In apoptotic progress of tumor cells stimulated by special agents, the calreticulin (CRT) was relocated from endoplasmic reticulum onto the cell surface. When used as cellular antigen to immunize experimental animals, these CRT-coated apoptotic tumor cells could initiate effective anti-tumor immunoresponse against homologous tumor cells, indicating the value of CRT in anti-tumor immunotherapy. In order to develop an universal technique that could make CRT-coating more efficiently in the tumor cells, in this study, a mouse CRT recombinant gene with virus G-protein coupled receptor (vGPCR) was constructed and cloned into vector pcDNA3.1(+). When resulted plasmid pcDNA3.1(+)-mCRT/ vGPCR was stably transfected into the mouse melanoma B16-F1 cells, the mCRT-vGPCR recombinant protein was synthesized. With the membrane-locating ability of vGPCR in the recombinant protein, mCRT-vGPCR was carried onto the surface of B16-F1 cells efficiently. Overexpression of mCRT-vGPCR on the cell surface could enhance the phagocytosis of B16-F1 by macrophages in vitro. When mCRT-vGPCR coated B16-F1 cells were used as a cell-antigen to immunize mice, the specific anti-tumor immune response against the homologous tumor cells was initiated efficiently. Our data in this study may provide a new possibility for CRT-mediated tumor immune prevention and treatment.
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Comparison of hypoglycemic activity of fermented mushroom of Inonotus obliquus rich in vanadium and wild-growing I. obliquus.
Biol Trace Elem Res
PUBLISHED: 03-03-2011
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The effects of vanadium-enriched and wild Inonotus obliquus were tested on hyperglycemic mice. The vanadium content of the culture medium was 0.6%, reaching a concentration of 3.0 mg/g in the cultured mushroom while in the wild variety is 1/100 of that amount. The toxicity of vanadium at the 3.0 mg/g level is negligible, but its anti-diabetic effects are significantly different to those of the wild variety (p?
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Displacement of bilirubin from albumin in plasma from jaundiced newborns. An in vitro study of purified Chinese herbal constituents and sulfisoxazole.
Phytother Res
PUBLISHED: 02-01-2011
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The aim of the present study was to clarify the in vitro potential of the purified Chinese herbal constituents LZX-A (neferine), QTJ (sinomenine), YHS (tetrahydropalmitine) and SQZG (notoginsenoside R1) to displace the highly bound bilirubin from albumin binding sites in plasma from jaundiced newborn infants. Sulfisoxazole (1.32?mM) was used as a positive control for bilirubin displacement. The displacing potential of the herbal constituents was investigated at assumed therapeutic concentrations and up to 100 times higher. Total (TB) and unbound (UB) bilirubin in plasma were measured by the peroxidase method. Sulfisoxazole increased the UB concentration in plasma by more than 60%. An increased % displacement of bilirubin was found at higher TB levels confirming the presence also of lower affinity binding sites for bilirubin in plasma. None of the purified herbal constituents showed any bilirubin displacing properties and were unaffected by the level of TB in plasma. The combination of sulfisoxazole and the herbal constituents showed no synergistic effect. It is concluded that none of the investigated purified herbal constituents possess any significant potential in vitro to increase the UB concentration in plasma from jaundiced newborn infants.
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[Pharmacology experimental study of new hematischesis compounds after Flos Sophorae carbonized].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 12-09-2010
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To search new effective compounds, the different hemostatic effects of Flos Sophorae, Flos Sophorae Carbonisatus and principal constituent were observed.
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[Pseudoanaphylactoid reaction analysis of Chinese herbal injections in Beagle dogs].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 12-09-2010
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To investigate the preclinical evaluation method of pseudoanaphylactoid reactions for Chinese herbal injections.
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[Detection of bacterial endotoxin content in eight kinds of injection by cytokine revulsion].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 10-12-2010
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By using RAW 264.7 macrophage cell line, we studied the dose-effect relationship of endotoxin induced RAW 264.7 cells to release TNF-alpha, and then detected the content of endotoxin in 8 kinds of injections, so that we can investigate the feasibility and the interference factors of the novel test.
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[Content detection of bacterial endotoxin in two kinds of injection by gelatin technique].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 09-09-2010
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To detect content of bacterial endotoxin in Yuxingcao and Qingkailing injections by specific and nonspecific tachypleus amebocyte lysate technique for in order to investigate the feasibility of specific tachypleus amebocyte lysate technique for detecting bacterial endotoxin in traditional Chinese drug injections.
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[Material and mechanisms induced pseudo allergic reactions of Yuxingcao injection].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 09-07-2010
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To investigate the characteristics, sensitizin and the mechanism of pseudo allergic reaction induced by Yuxingcao injection.
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Mitoxantrone-mediated apoptotic B16-F1 cells induce specific anti-tumor immune response.
Cell. Mol. Immunol.
PUBLISHED: 12-17-2009
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In the process of cell apoptosis induced by specific reagents, calreticulin (CRT) in endoplasmic reticulum is transferred and coated onto the cell membrane. As a sort of specific ligand, the CRT on the surface of apoptotic cells could mediate recognition and clearance of apoptotic cells by phagocytes. In this research we discovered that mitoxantrone could induce apoptosis of mouse melonoma B16-F1 tumor cells, accompanied by the membrane translocation and coating of CRT. When mitoxantrone-treated B16-F1 cells were used as antigen to inoculate mice, the mice acquired an ability to suppress proliferation of homologous tumor cells. Splenocytes from these mice showed an increased cytolytic effect on homologous B16-F1 cells but no such effect on non-homologous H22 tumor cells. All these results suggested that mitoxantrone-treated apoptotic B16-F1 cells could be used as a sort of cell vaccine to initiate effective anti-tumor immunoresponse in mice.
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[Study on embryo toxicity of Cinnabaris].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 12-03-2009
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To observe the effect of Cinnabaris on mouse embryos after pregnant mice were treated by Cinnabaris in different periods of pregnancy.
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[Anti-thrombosis effect and its mechanism of Qingkailing injection].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 09-26-2009
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To investigate the anti-thrombosis effect and its mechanism of Qingkailing injection (QKL).
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[Study of mercury cumulation in Cinnabar-treated rats].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 07-27-2009
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To investigate the mercury cumulation following single dose or long-term use of Cinnabar to rats.
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[Omparative study on allergen assessment animal models in Brown Norway rat and guinea pig].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 05-23-2009
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To compare the sensitivity of Brown Norway rats (BN) with Guinea pigs (GP) as allergen assessment animal models.
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[Study on hepatoxicity and nephrotoxicity of cinnabar in rats].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 05-19-2009
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To investigate hepatoxicity and nephrotoxicity of cinnabar to provide the scientific basis for safe uses in clinic.
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Whole-cell vaccine coated with recombinant calreticulin enhances activation of dendritic cells and induces tumour-specific immune responses.
Oncol. Rep.
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It has been reported that calreticulin (CRT) plays an important role in mediating immunogenic tumour cell death. In the process of tumour cell apoptosis induced by specific stimuli, CRT is quickly transferred from the endoplasmic reticulum to the cell membrane. As a specific ligand, CRT on the surface of apoptotic tumour cells could mediate the recognition and clearance of apoptotic tumour cells by professional and non-professional phagocytes. In our previous studies, we used B16-F1 mouse melanoma cells coated with mCRT-vGPCR (a recombinant fusion protein of mouse CRT and virus G-protein-coupled receptor) as a whole-cell tumour vaccine to immunise experimental animals and found that this whole-cell vaccine could strongly inhibit the growth of homologous tumours. In this study, we further evaluated immune responses induced by this mCRT-vGPCR-coated whole-cell vaccine both in vivo and in vitro. An in vitro phagocytosis assay showed that the mCRT-vGPCR on the cell surface greatly enhanced the engulfment of B16-F1 cells by dendritic cells (DCs). The specific antitumour immune response was observed when the mCRT-vGPCR-coated B16-F1 cells were used as a whole-cell tumour vaccine to immune mice, which included significantly enhanced cytotoxic T lymphocyte (CTL) activities and increased the number of IFN-?-producing T cells. These results indicate that the mCRT-vGPCR-coated whole-cell vaccine can induce specific antitumour immunity though the activation of DCs. These results may provide an experimental basis for the development of new tumour vaccines.
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[Development of gastric precancerous lesion animal model].
Zhongguo Zhong Yao Za Zhi
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To establish a model of gastric precancerous lesion by using Aristolochic manshuriensis which contains aristolochic acids.
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An investigation of the anti-diabetic effects of an extract from Cladonia humilis.
Pak J Pharm Sci
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The effect of Cladonia humilis on glycaemic metabolism was researched in this studied. The blood glucose, insulin secretion and glycogen synthesis of the hyperglycemic mice induced by alloxan were analyzed respectively. The gluconeogenesis and the sugar tolerance of the normal mice were also analyzed in this paper. After the hyperglycemic mice were orally administrated with Cladonia humilis extract, the blood glucose was decreased (p<0.05), the level of insulin secretion and glycogen synthesis were elevated (p<0.05, p<0.01, respectively). In addition, Cladonia humilis extract could inhibit the gluconeogenesis (p<0.01) and improve the sugar tolerance in normal control mice. These results maybe account for the causes of Cladonia humilis extract-induced significant decreases of the blood glucose in hyperglycemic mice.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.