JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Phylogeny and molecular evolution analysis of PIN-FORMED 1 in angiosperm.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
PIN-FORMED 1 (PIN1) is an important secondary transporter and determines the direction of intercellular auxin flow. As PIN1 performs the conserved function of auxin transport, it is expected that the sequence and structure of PIN1 is conserved. Therefore, we hypothesized that PIN1 evolve under pervasive purifying selection in the protein-coding sequences in angiosperm. To test this hypothesis, we performed detailed evolutionary analyses of 67 PIN1 sequences from 35 angiosperm species. We found that the PIN1 sequences are highly conserved within their transmembrane regions, part of their hydrophilic regions. We also found that there are two or more PIN1 copies in some of these angiosperm species. PIN1 sequences from Poaceae and Brassicaceae are representative of the modern clade. We identified 12 highly conserved motifs and a significant number of family-specific sites within these motifs. One family-specific site within Motif 11 shows a different residue between monocots and dicots, and is functionally critical for the polarity of PIN1. Likewise, the function of PIN1 appears to be different between monocots and dicots since the phenotype associated with PIN1 overexpression is opposite between Arabidopsis and rice. The evolution of angiosperm PIN1 protein-coding sequences appears to have been primarily driven by purifying selection, but traces of positive selection associated with sequences from certain families also seem to be present. We verified this observation by calculating the numbers of non-synonymous and synonymous changes on each branch of a phylogenetic tree. Our results indicate that the evolution of angiosperm PIN1 sequences involve strong purifying selection. In addition, our results suggest that the conserved sequences of PIN1 derive from a combination of the family-specific site variations and conserved motifs during their unique evolutionary processes, which is critical for the functional integrity and stability of these auxin transporters, especially in new species. Finally, functional difference of PIN1 is likely to be present in angiosperm because the positive selection is occurred in one branch of Poaceae.
Related JoVE Video
[Technical standards of traditional Chinese medicine industry].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 09-07-2010
Show Abstract
Hide Abstract
Basing on the basic theory of traditional Chinese medicine (TCM), the technical standards of TCM industry is such a Link that it is to clarify the internal relations between the basic theory of TCM and developing of TCM industry. This article analyzed several problems of technical standards of TCM industry, such as basic theory of TCM and standardization problem of TCM industry. Technical standards of TCM industry must receive the guidance of basic theory of TCM, so that it will promote the process of modernization and internationalization of TCM industry.
Related JoVE Video
[Marburg I polymorphism of Factor VII-activating protease and cerebral infarction].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 11-17-2009
Show Abstract
Hide Abstract
To determine the relation between Marburg I polymorphism of Factor VII-activating protease (FSAP) and cerebral infarction,and to analyze whether it is one of the risk factors of cerebral infarction.
Related JoVE Video
Effect of alphaIIb Ala477Pro(A446P) mutation of the platelet glycoprotein on the expression of alphaIIbbeta3 complex.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 09-08-2009
Show Abstract
Hide Abstract
To explore the effect of glycoprotein (GP) alphaIIb Ala477Pro(A446P) mutation on the expression of integrin alphaIIbbeta3.
Related JoVE Video
Detection of myeloperoxidase activity in primary leukemic cells by an enhanced chemiluminescent assay for differentiation between acute lymphoblastic and non-lymphoblastic leukemia.
Clin. Chim. Acta
PUBLISHED: 01-22-2009
Show Abstract
Hide Abstract
Myeloperoxidase (MPO) plays a crucial role in the differentiation of acute lymphoblastic leukemia (ALL) and acute non-lymphoblastic leukemia (ANLL). In this report, we proposed the application of the enhanced chemiluminescent (ECL) technique to the determination of MPO activity in blasts of acute leukemia (AL).
Related JoVE Video
[In vitro selection of single strand deoxyribonucleic acid aptamers binding to cells from patients with acute myeloblastic leukemia].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
Show Abstract
Hide Abstract
To screen aptamers binding CD33+/CD34- cells from patients with acute myeloblastic leukemia M2 subtype (AML-M2).
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.