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Find video protocols related to scientific articles indexed in Pubmed.
Fractional extreme value adaptive training method: fractional steepest descent approach.
IEEE Trans Neural Netw Learn Syst
PUBLISHED: 03-21-2015
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The application of fractional calculus to signal processing and adaptive learning is an emerging area of research. A novel fractional adaptive learning approach that utilizes fractional calculus is presented in this paper. In particular, a fractional steepest descent approach is proposed. A fractional quadratic energy norm is studied, and the stability and convergence of our proposed method are analyzed in detail. The fractional steepest descent approach is implemented numerically and its stability is analyzed experimentally.
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Endovascular embolization for managing anastomotic bleeding after stapled digestive tract anastomosis.
Acta Radiol
PUBLISHED: 11-20-2014
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Anastomotic bleeding is an infrequent but life-threatening complication after stapled digestive tract anastomosis. Endovascular embolization is one of the available treatments, but precise clinical outcomes are yet to be evaluated.
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The chemotaxis of M1 and M2 macrophages is regulated by different chemokines.
J. Leukoc. Biol.
PUBLISHED: 11-01-2014
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The homing of proinflammatory (M1) and the "alternatively activated" anti-inflammatory (M2) macrophages plays a different role in the process of inflammation. Chemokines are the major mediators of macrophage chemotaxis, but how they differentially regulate M1 and M2 macrophages remains largely unclear. In the present study, we attempted to screen chemokines that differentially induce chemotaxis of M1 and M2 macrophages and to explore the underlying mechanism. Among the 41 chemokines that specifically bind to 20 chemokine receptors, CCL19, CCL21, CCL24, CCL25, CXCL8, CXCL10, and XCL2 specifically induced M1 macrophage chemotaxis, whereas CCL7 induced chemotaxis of both M1 and M2 macrophages. Whereas the differential effects of these chemokines on M1/M2 macrophage chemotaxis could be attributable to the predominant expression of their cognate receptors on the macrophage subsets, CCR7, the receptor for CCL19/CCL21, appeared to be an exception. Immunoblot analysis indicated an equivalent level of CCR7 in the whole cell lysate of M1 and M2 macrophages, but CCL19 and CCL21 only induced M1 macrophage chemotaxis. Both immunoblot and confocal microscopy analyses demonstrated that CCR7 was predominantly expressed on the cell surface of M1 but in the cytosol of M2 macrophages before ligand stimulation. As a result, CCL19 or CCL21 induced activation of both MEK1-ERK1/2 and PI3K-AKT cascades in M1 but not in M2 macrophages. Intriguingly, CCL19/CCL21-mediated M1 macrophage chemotaxis was blocked by specific inhibition of PI3K rather than MEK1. Together, these findings suggest that recruitment of M1 and M2 macrophages is fine tuned by different chemokines with the involvement of specific signaling pathways.
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[Effects of low molecular weight organic acids on speciation of exogenous Cu in an acid soil].
Huan Jing Ke Xue
PUBLISHED: 10-24-2014
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In order to ascertain the effect of LMWOA (citric acid, tartaric acid, oxalic acid) on Cu-contaminated soils and to investigate the change of Cu species, a red soil derived from quartz sandstone deposit was added by Cu (copper) in the form of CuSO4 x 5H2O so as to simulate soil Cu pollution, keeping the additional Cu concentrations were 0, 100, 200, 400 mg x kg(-1) respectively. After 9 months, different LMWOA was also added into the simulated soil, keeping the additional LMWOAs in soil were 0, 5, 10, 20 mmol x kg(-1) respectively. After 2 weeks incubation, the modified sequential extraction method on BCR (European Communities Bureau of Reference) was used to evaluate the effects of these LMWOAs on the changes of copper forms in soil. The result showed that the percentage of weak acid dissolved Cu, the most effective form in the soil increased with three organic acids increase in quantity in the simulated polluted soil. And there was a good activation effect on Cu in the soil when organic acid added. Activation effects on Cu increased with concentration of citric acid increasing, but it showed a rise trend before they are basically remained unchanged in the case of tartaric acid and oxalic acid added in the soil. On the contrary, the state of the reduction of copper which was regarded as a complement for effective state decreased with the increased concentration of organic acid in the soil, especially with citric acid. When 20 mmol x kg(-1) oxalic acid and citric acid were added into the soil, the activation effect was the best; whereas for tartaric, the concentration was 10 mmol x kg(-1). In general, the effect on the changes of Cu forms in the soil is citric acid > tartaric acid > oxalic acid.
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[Effect of advanced glycosylation end products on oxidative stress and MCP-1 in human renal mesangial cells].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
PUBLISHED: 10-22-2014
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To investigate the effects of advanced glycosylation end products(AGEs)modified bovine serum albumin (AGE-BSA) on the expression of reactive oxygen species (ROS) and monocyte chemoattractant protein-1 (MCP-1) in human renal mesangial cells (HRMCs).
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Proposed fast performance evaluation of an imaging system with a discrete detector array.
Appl Opt
PUBLISHED: 10-17-2014
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This paper proposes a newly developed fast measurement of a modulation transfer function (MTF) optical system inclusive of on-axis and off-axis measurement. First, we discuss how a description of an imager in terms of its optical transfer function is not appropriate for a discrete imaging system when aliasing occurs, since these optical systems transform high spatial frequencies into low frequencies; we then measure how an efficient microscanning method could remove the aliasing effects from assigned telecentric optics and nontelecentric optics. A knife edge function and a slit function as a light source are employed in this measurement. The experiment with the newly designed MTF measurement system synchronizes on-axis and off-axis measurement. In addition, a microscan method with a specially written macro is introduced in this experiment to eliminate aliasing effects. After simulation and experimental analysis, first the slit function as a target delivers decent MTF repeatability for this newly developed MTF measurement system, which synchronizes with on-axis and off-axis measurement simply in 2 s after all equipment is ready and aligned. Second, after the six-step microscanning, aliasing will be eliminated to near zero in most cases. Finally, it is concluded that during the microscan, there is no difference between telecentric and nontelecentric optics.
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Coculture of Peripheral Blood-Derived Mesenchymal Stem Cells and Endothelial Progenitor Cells on Strontium-Doped Calcium Polyphosphate Scaffolds to Generate Vascularized Engineered Bone.
Tissue Eng Part A
PUBLISHED: 10-10-2014
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Vascularization of engineered bone tissue is critical for ensuring its survival after implantation and it is the primary factor limiting its clinical use. A promising approach is to prevascularize bone grafts in vitro using endothelial progenitor cells (EPC) derived from peripheral blood. Typically, EPC are added together with mesenchymal stem cells (MSC) that differentiate into osteoblasts. One problem with this approach is how to promote traditional tissue engineering bone survival with a minimally invasive method. In this study, we examined the effectiveness of administering to stimulate the release of peripheral blood stem cells and their co-culturing system for generating prevascularized engineered bone. Cells were isolated by Ficoll density gradient centrifugation and identified as EPC and MSC based on morphology, surface markers, and functional analysis. EPC and MSC were cocultured in several different ratios, and cell morphology and tube formation were assessed by microscopy. Expression of osteogenesis and vascularization markers was quantified by enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction, and histochemical and immunofluorescence staining. Increasing the proportion of EPC in the coculture system led to greater tube formation and greater expression of the endothelial cell marker CD31. An EPC:MSC ratio of 75:25 gave the highest expression of osteogenesis and angiogenesis markers. Cocultures adhered to a three-dimensional scaffold of strontium-doped calcium polyphosphate and proliferated well. Our findings show that coculturing peripheral blood-derived EPC and MSC may prove useful for generating prevascularized bone tissue for clinical use.
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Discovery of 2-[5-(4-Fluorophenylcarbamoyl)pyridin-2-ylsulfanylmethyl]phenylboronic Acid (SX-517): Noncompetitive Boronic Acid Antagonist of CXCR1 and CXCR2.
J. Med. Chem.
PUBLISHED: 10-08-2014
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The G protein-coupled chemokine receptors CXCR1 and CXCR2 play key roles in inflammatory diseases and carcinogenesis. In inflammation, they activate and recruit polymorphonuclear cells (PMNs) through binding of the chemokines CXCL1 (CXCR1) and CXCL8 (CXCR1 and CXCR2). Structure-activity studies that examined the effect of a novel series of S-substituted 6-mercapto-N-phenyl-nicotinamides on CXCL1-stimulated Ca(2+) flux in whole human PMNs led to the discovery of 2-[5-(4-fluorophenylcarbamoyl)pyridin-2-ylsulfanylmethyl]phenylboronic acid (SX-517), a potent noncompetitive boronic acid CXCR1/2 antagonist. SX-517 inhibited CXCL1-induced Ca(2+) flux (IC50 = 38 nM) in human PMNs but had no effect on the Ca(2+) flux induced by C5a, fMLF, or PAF. In recombinant HEK293 cells that stably expressed CXCR2, SX-517 antagonized CXCL8-induced [(35)S]GTP?S binding (IC50 = 60 nM) and ERK1/2 phosphorylation. Inhibition was noncompetitive, with SX-517 unable to compete the binding of [(125)I]-CXCL8 to CXCR2 membranes. SX-517 (0.2 mg/kg iv) significantly inhibited inflammation in an in vivo murine model. SX-517 is the first reported boronic acid chemokine antagonist and represents a novel pharmacophore for CXCR1/2 antagonism.
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Baicalin attenuates TNBS-induced colitis in rats by modulating the Th17/Treg paradigm.
Arch. Pharm. Res.
PUBLISHED: 09-23-2014
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Baicalin, a flavonoid, has a wide range of pharmacological properties, including immunomodulation. The objective of this study was to investigate the effect of baicalin on the balance of T helper 17 (Th17) and regulatory T (Treg) cells in a colitis model. The rat colitis model was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). Baicalin (10 ml/kg, each) or mesalazine (positive control) was then administered orally for 7 days. Inflammatory and immunological responses were evaluated by pathology, enzyme-linked immunosorbent assay, real-time polymerase chain reaction, western blot analysis, and flow cytometry. Our study showed that baicalin not only significantly attenuated TNBS-induced colitis by reducing the disease activity index as well as macroscopic and microscopic scores, but it also improved the weight loss and shortening of the colon. Baicalin treatment also induced a significant decrease in the levels of inflammatory mediators, including the myeloperoxidase activity, the levels of tumor necrosis factor ?, IL-1?, and Th1-related cytokines IL-12 and IFN-?. Furthermore, the beneficial effects of baicalin seem to be associated with regulation of the Th17 and Treg paradigm. We found that administration of baicalin significantly downregulated the number of Th17 cells and the levels of Th17-related cytokines (IL-17 and IL-6) and retinoic acid receptor-related orphan receptor ?t. In contrast, there was an increase in Treg cells numbers, Treg-related cytokines transforming growth factor-? and IL-10, and forkhead box P3. Our results suggest that the anti-inflammatory effect of baicalin may be linked to modulation of the balance between Th17 and Treg cells in TNBS-induced ulcerative colitis.
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X-linked inhibitor of apoptosis-associated factor l (XAFl) enhances the sensitivity of colorectal cancer cells to cisplatin.
Med. Oncol.
PUBLISHED: 09-12-2014
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The purpose of present study was to investigate the roles of X-linked inhibitor of apoptosis-associated factor l (XAFl) in regulation apoptosis of colorectal cancer (CRC) cells after treatment with cisplatin (DDP). A total of ten paired cancerous and non-cancerous tissues were collected from patients with CRC after surgery. The levels of XAFl protein were detected by Western blot. Primary CRC cells were separated from cancer tissues, and its viability or apoptosis after treatment with DDP was determined with MTT or Annexin V/PI assays, respectively. Furthermore, we either up-regulated transfecting a XAF1 overexpression vector or down-regulated XAF1 by siRNA interference. And then, the XAF1 levels and its sensitivity to cisplatin were assessed. XAFl had a lower expression in the cancerous tissues from samples T1, T2 and T3 than their paired non-cancerous tissues N1, N2 and N3. However, the expression of XAF1 was not detected in samples T4 and N1. XAF1 levels in cancer tissues significantly decreased in comparison with normal tissues. Cell abilities of primary cells were significantly decreased in a dose-dependent manner, after treatment with a series concentrations of cisplatin (2, 5, 10 ?g/mL) for 48 h. Although, after down-expression of XAFl by siRNA, cisplatin caused a significant decreases in apoptosis rates in CRC cells. The up-regulation of XAF1 distinctly increased apoptosis in CRC cells administered by cisplatin (P < 0.001). The XAFl could promoted apoptosis and enhanced chemotherapy sensitivity to cisplatin in CRC cells.
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Intra-arterial calcium stimulation test with hepatic venous sampling for preoperative diagnosis of a large insulinoma in an obese young man.
Singapore Med J
PUBLISHED: 09-06-2014
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Herein, we report the case of a large benign insulinoma in an obese young man with a three-year history of asymptomatic hypoglycaemia. He presented to our outpatient department with a two-week history of dizziness and morning cold sweats. A random serum glucose test revealed hypoglycaemia. Upon admission, computed tomography and magnetic resonance imaging of the abdomen with intravenous contrast media showed an enhancing mass lesion in the uncinate process of the pancreas. To confirm the diagnosis, an intra-arterial calcium stimulation test with hepatic venous sampling was performed for preoperative localisation and to exclude the presence of occult insulinomas. The patient underwent an exploratory laparotomy, with successful resection of the pancreatic head tumour. Histology confirmed the diagnosis of insulinoma. The patient's postoperative recovery was uneventful, and he has not developed further episodes of hypoglycaemia three years post surgery.
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Tissue-engineered ribs for chest wall reconstruction: a case with 12-year follow-up.
Regen Med
PUBLISHED: 08-28-2014
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We hereby report on a case in which a huge chest wall defect generated by resection of a massive aggressive tumor (desmoplastic fibroma) was repaired with osteogenic-induced mesenchymal stem cells embedded in a bone-derived biomaterial. In this case, there were three challenges to overcome: reconstruction of the soft tissue, repair of the skeletal defect of the thoracic wall and repair of the defect in the pleural cavity. The defects of soft tissue and pleural cavity were reconstructed, respectively, with an ipsilateral abdominal flap and a diaphragm muscular flap. The huge defect in the chest wall was successfully repaired with the tissue-engineered ribs, which was confirmed by long-term follow-up with computerized tomography and histological and immunohistochemical evaluations. In view of its effectiveness and safety, tissue-engineered bones may have a broad application for the repair of large skeletal defects and bone regeneration.
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Brocazines A-F, Cytotoxic Bisthiodiketopiperazine Derivatives from Penicillium brocae MA-231, an Endophytic Fungus Derived from the Marine Mangrove Plant Avicennia marina.
J. Nat. Prod.
PUBLISHED: 08-08-2014
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Six new disulfide-bridged diketopiperazine derivatives, brocazines A-F (1-6), along with one known analogue (7), were isolated and identified from the cytotoxic extract of Penicillium brocae MA-231, a fungus obtained from the fresh tissue of the marine mangrove plant Avicennia marina. The structures of these compounds were established on the basis of detailed interpretation of NMR and mass spectroscopic data. X-ray crystallographic analysis confirmed the structure of 1 and established the structure and absolute configuration of 5, while the absolute configurations for compounds 1, 4, and 6 were deduced by comparison of the CD data with those of 5. Compounds 1, 2, 5, and 6 showed cytotoxic activities against several tumor cell lines.
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Progesterone and norgestrel alter transcriptional expression of genes along the hypothalamic-pituitary-thyroid axis in zebrafish embryos-larvae.
Comp. Biochem. Physiol. C Toxicol. Pharmacol.
PUBLISHED: 07-15-2014
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The aim of this study was to investigate the effects of progestins on the hypothalamic-pituitary-thyroid (HPT) axis in the early stage of zebrafish. Zebrafish embryos were exposed to progesterone (P4) or norgestrel (NGT) at 5, 50 and 100ngL(-1) for 144h post fertilization (hpf), and the transcriptional levels of target genes along the hypothalamic-pituitary-thyroid axis were determined daily. The results showed that P4 had only minor effects on the mRNA expression of thyroglobulin (Tg), iodothyronine deiodinase type ? (Dio1) and thyroid hormone receptor ? (Thrb) genes. Similarly, the effects of NGT on transcripts of thyrotropin-releasing hormone (Trh), Dio1, iodothyronine deiodinase type II (Dio2) and thyroid hormone receptor ? (Thra) genes were generally low. In addition, NGT resulted in some alterations of Tg and Thrb transcripts at different time points. However, a strong induction of Nis mRNA by P4 and NGT was observed in zebrafish embryos-larvae. The overall results showed that besides Nis no effects on the hypothalamic-pituitary-thyroid (HPT) axis are observed following exposure to P4 and NGT, which imply that both P4 and NGT have potential effects on the thyroid endocrine system by inducing transcript of Nis gene during the early stage of zebrafish.
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Assessment of cardiac output and volume load by transpulmonary thermodilution technique in immature pigs.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 07-07-2014
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To assess the accuracy of cardiac output(CO)measured by transpulmonary thermodilution technique(TPTD)and explore the validity of intrathoracic blood volume index(ITBVI)for assessment of circulatory volume status.
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Insight into astrocyte activation after optic nerve injury.
J. Neurosci. Res.
PUBLISHED: 06-04-2014
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In response to optic nerve damage, astrocytes become reactive. This reactivity can be identified by the presence of morphological and molecular changes throughout the retina and optic nerve as well as the formation of a glial scar. The process of astrocyte activation exhibits spatial and temporal characteristics, and it is finely regulated by complex signaling mechanisms. Excessive astrocyte activation plays a crucial role in progressive optic nerve injury. This review focuses on the spatial and temporal characteristics and mechanisms of astrocyte activation and discusses the modulation of astrocyte activation. Further insight into astrocyte activation might provide targets for future therapeutic interventions. © 2014 Wiley Periodicals, Inc.
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Effect of Tiotropium on Heart Rate Variability in Stable Chronic Obstructive Pulmonary Disease Patients.
J Aerosol Med Pulm Drug Deliv
PUBLISHED: 05-21-2014
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Abstract Background: The chronic use of the long-acting anticholinergic agent, tiotropium, in chronic obstructive pulmonary disease (COPD) has been linked in some reports to an increase in adverse cardiovascular effects. Decreased heart rate variability (HRV) is a condition seen in COPD patients that has also been linked to poor cardiovascular outcome. We aimed in this study to investigate changes in HRV caused by tiotropium administration to COPD patients in order to determine whether changes occurred that might contribute to an increase in adverse cardiovascular events. Methods: Seventy patients with moderate-to-severe stable COPD were treated with once-daily dosing of tiotropium (two puffs of Spiriva Respimat, 2.5??g solution) for 3 months. HRV, pulmonary function, and quality of life were measured before and after 1 and 3 months of therapy. Results: Pulmonary function and quality of life improved significantly, after both 1 and 3 months of therapy. No significant change in HRV parameters occurred, except for a significant decrease in the high-frequency and increase in the low-frequency component of HRV at the 1-month assessment. Conclusion: Changes in HRV caused by tiotropium use are not sufficient to explain a possible increase in adverse cardiovascular events.
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miR-10b is overexpressed in hepatocellular carcinoma and promotes cell proliferation, migration and invasion through RhoC, uPAR and MMPs.
J Transl Med
PUBLISHED: 05-14-2014
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BackgroundRecently, miR-10b is identified as a miRNA highly expressed in many human cancers, promoting cell migration and invasion. However, the specific function of miR-10b in hepatocellular carcinoma (HCC) is unclear at this point.MethodsThe miR-10b expression levels in 60 paired different TNM Stage HCC tumor tissues compared with adjacent non-tumor (ANT) tissues, normal tissue control (8 benign tumor and 7 normal liver tissues), 3 normal liver and 7 HCC cell lines were measured by real-time quantitative RT-PCR and to evaluate their association with HCC clinicopathologic features. Invasion assay, MTT proliferation assay and wound-healing assay were performed to test the invasion and proliferation of HCC cell after transfection. The effect of miR-10b on HCC in vivo was validated by murine xenograft model.ResultsWe found that miR-10b expression was increased in human HCC tissues and cell lines compared with normal control, respectively. The expression of miR-10b was correlated with HCC metastatic ability. Overexpression of miR-10b in MHCC-97 L cells increased cell motility and invasiveness, whereas inhibition of miR-10b in MHCC-97H cells reduced cell motility and invasiveness in vitro and in vivo. We also showed that HOXD10 was negatively regulated by miR-10b at the posttranscriptional level, via a specific target site within the 3¿UTR by luciferase reporter assay. Furthermore, we found that miR-10b induced HCC cell invasion and migration by modulating the HOXD10 target gene RhoC, uPAR, MMP-2 and MMP-9 expression.ConclusionsOur results suggested that miR-10b was overexpressed in HCC and promoted HCC cell migration and invasion through the HOXD10/ RhoC/ uPAR/ MMPs pathway which may provide a novel bio-target for HCC therapy.
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A time-course transcriptional kinetics of the hypothalamic-pituitary-gonadal and hypothalamic-pituitary-adrenal axes in zebrafish eleuthero-embryos after exposure to norgestrel.
Environ. Toxicol. Chem.
PUBLISHED: 05-07-2014
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The objective of this study was to investigate the effects of norgestrel (NGT) on the hypothalamic-pituitary-gonadal (HPG) and hypothalamic-pituitary-adrenal (HPA) axes in zebrafish eleuthero-embryos. Zebrafish embryos were exposed to different concentrations of NGT (0, 5, 50 and 100 ng L(-1) ) for 144 h post fertilization (hpf), and the transcriptional profiles of the HPG and HPA axes were examined every day. NGT modulated the expression of Pgr and Vtg1 mRNAs mainly at 96 hpf for all treatment groups. In addition, NGT strongly altered the expression of Cyp11a1 mRNA above 5 ng L(-1) (significant up-regulation from 48 to 120 hpf and significant down-regulation for 144 hpf). NGT treatment could significantly induce expression of Cyp19a1a, Cyp11b, Gnrh2, Gnrh3 and Lhb mRNAs, but inhibit transcripts of Hsd11b2 and Crh genes above 5 ng L(-1) at different time points. The transcriptional expression levels of Esr1, Ar, Star, Hsd17b3, Fshb, and Pomc were also mediated by 5 ng L(-1) NGT or higher during different exposure periods. Taken together, the overall results imply that the transcriptional changes in zebrafish eleuthero-embryos may pose a potential effect on embryonic development, in particular the brain and gonadogenesis. Environ Toxicol Chem © 2014 SETAC.
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Contamination profiles of perfluoroalkyl substances in five typical rivers of the Pearl River Delta region, South China.
Chemosphere
PUBLISHED: 05-05-2014
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A survey on contamination profiles of eighteen perfluoroalkyl substances (PFASs) was performed via high performance liquid chromatography-tandem mass spectrometry for surface water and sediments from five typical rivers of the Pearl River Delta region, South China in summer and winter in 2012. The total concentrations of the PFASs in the water phase of the five rivers ranged from 0.14 to 346.72 ng L(-1). The PFAS concentrations in the water phase were correlated positively to some selected water quality parameters such as chemical oxygen demand (COD) (0.7913) and conductivity (0.5642). The monitoring results for the water samples showed significant seasonal variations, while those for the sediment samples showed no obvious seasonal variations. Among the selected 18 PFASs, perfluorooctane sulfonic acid (PFOS) was the dominant PFAS compound both in water and sediment for two seasons with its maximum concentration of 320.5 ng L(-1) in water and 11.4 ng g(-1) dry weight (dw) in sediment, followed by perfluorooctanoic acid (PFOA) with its maximum concentration of 26.48 ng L(-1) in water and 0.99 ng g(-1) dw in sediment. PFOS and PFOA were found at relatively higher concentrations in the Shima River and Danshui River than in the other three rivers (Xizhijiang River, Dongjiang River and Shahe River). The principal component analysis for the PFASs concentrations in water and sediment separated the sampling sites into two groups: rural and agricultural area, and urban and industrial area, suggesting the PFASs in the riverine environment were mainly originated from industrial and urban activities in the region.
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[Characteristics of spatial variation of soil nutrients in sloping field in a gorge karst region, southwest China].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 04-29-2014
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Based on a grid (20 m x 20 m) sampling, spatial heterogeneity and pattern of soil nutrients in sloping field in the gorge karst region, southwestern China, were explored by using classical statistics and geostatistics methods. The results showed that soil nutrient contents in slope field in the canyon karst region were more abundant, where pH value had a weak variation and the soil organic matter (SOM) had a moderate degree of variation. All the soil nutrients had moderate or strong variation with an order of available phosphorus (AP) > total potassium (TK) > SOM > alkaline nitrogen (AN) > total nitrogen (TN) > total phosphorus (TP) > available potassium (AK). All of the soil nutrients had a good spatial autocorrelation and the autocorrelation function performed in the same law of developing from positive to negative direction with the inflection point ranged from 80 to 100 m. In addition, the Moran's I was small for TK and AP while large for other nutrients. Characteristics of spatial variation differed among soil nutrients. Exponential model fitted best for TK and AP, in which the ratio of nugget to sill (C0/(C0 + C)) and the range (A) were small and the fractal dimension (D) was high, showed a strong spatial correlation. Spherical model fitted best for other soil nutrients, with C0/(C0 + C) , the range (A) and D showing a moderate autocorrelation. Kriging analysis clearly indicated that pH, SOM, TN, TP and AN were distributed in a concave pattern, while AP and AK had fragmented patch distribution. Therefore, vegetation, topography, human disturbance and strong heterogeneity of microhabitats are main factors leading to the differences in patterns of soil nutrients on the sloping land in the gorge karst region.
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Pulse oximetry with clinical assessment to screen for congenital heart disease in neonates in China: a prospective study.
Lancet
PUBLISHED: 04-22-2014
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Several pioneering studies have provided evidence for the introduction of universal pulse oximetry screening for critical congenital heart disease. However, whether the benefits of screening reported in studies from high-income countries would translate with similar success to low-income countries is unknown. We assessed the feasibility and reliability of pulse oximetry plus clinical assessment for detection of major congenital heart disease, especially critical congenital heart disease, in China.
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Roles of resolvins in the resolution of acute inflammation.
Cell Biol. Int.
PUBLISHED: 04-06-2014
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Resolution is an active process that terminates inflammatory response to maintain health. Acute inflammation and its timely resolution are important in host response to danger signals. Unresolved inflammation is associated with widely recurrent diseases. Resolvins, including the D and E series, are endogenous lipid mediators generated during the resolution phase of acute of inflammation from the ?-3 PUFAs, DHA, and EPA. They have anti-inflammatory and pro-resolving properties that have been determined in many inflammation studies in animal models. In this review, we provide an updated overview of biosynthesis, actions, and signaling pathways of resolvins, thereby underscoring their diverse protective roles and introducing novel therapeutic strategies for inflammation-associated diseases.
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Noninvasive measurement of glucose in artificial plasma with near-infrared and Raman spectroscopy.
Appl Spectrosc
PUBLISHED: 04-04-2014
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The goal of this research was to develop a method for noninvasive blood glucose assay. Near-infrared (NIR) spectroscopy and Raman spectroscopy, two more promising techniques compared to other methods, were investigated in two kinds of artificial plasma (AP). Calibration models were generated by performing partial least squares (PLS) regression and optimized individually by considering spectral range, spectral pretreatment methods, and number of model factors. The two spectroscopic models were validated for the determination of glucose, and the results show that the two spectroscopic models established are robust, accurate, and repeatable. Compared to Raman spectroscopy, the performance of NIR spectroscopy was much better, with lower root mean square errors of cross-validation (RMSECV) of 0.128 and 0.094 mg/ml, lower root mean square errors of validation (RMSEP) of 0.061 and 0.046 mg/ml, higher correlation coefficients (R) of 99.15% and 99.55%, and higher residual predictive deviations (RPD) of 10.8 and 15.0 for artificial plasma I and II, respectively.
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Expression patterns of metallothionein, cytochrome P450 1A and vitellogenin genes in western mosquitofish (Gambusia affinis) in response to heavy metals.
Ecotoxicol. Environ. Saf.
PUBLISHED: 04-03-2014
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The aim of this study was to evaluate the effects of three metals (Zn, Cd and Pb) on hepatic metallothionein (MT), cytochrome P450 1A (CYP1A) and vitellogenin (Vtg) mRNA expression in the liver of adult female mosquitofish (Gambusia affinis) after 1, 3 or 8d. Both concentration-response and time-course effects of hepatic MT, CYP1A and Vtg at the transcription level were determined by quantitative real-time PCR. The results from this study showed that Zn, Cd and Pb could significantly induced MT, CYP1A and Vtg mRNA expression levels in mosquitofish. In general, this study demonstrated that heavy metals modulate MT, CYP1A and Vtg mRNA expression levels in a metal-, concentration- or time-dependent manner.
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Gastric hepatoid adenocarcinoma: A computed tomography report of six cases.
World J. Gastroenterol.
PUBLISHED: 03-22-2014
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We describe the computed tomography (CT) imaging findings in six cases (five males and one female; age range 61-78 years; mean age 67.3 years) with histologically proven hepatoid adenocarcinoma of the stomach (HAS). Five of the six patients had elevated serum alpha-fetoprotein levels. The most common type of gross appearance HAS on CT is a polypoid mass (83%, 5/6). The most common contrast enhancement pattern was heterogeneous. All six patients had a regional lymphadenopathy larger than 6 mm in its short axis. Liver metastases (n = 3) were noted. Venous tumor thrombosis was identified in the portal vein (n = 2) of the regions near primary gastric tumors or metastatic masses. Our findings suggest in an elderly, male patients with a large heterogeneous enhancement tumor, the presence of distant metastases, regional lymphadenopathy and characteristically increased serum alpha-fetoprotein levels indicates a high likelihood of HAS.
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Rotator cuff repair using a decellularized tendon slices graft: an in vivo study in a rabbit model.
Knee Surg Sports Traumatol Arthrosc
PUBLISHED: 02-20-2014
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Although varieties of surgical repair techniques and materials have been used to repair rotator cuff defects, re-tearing frequently occurs. The purpose of this study is to evaluate the postoperative outcomes of rotator cuff repairs with a decellularized tendon slices (DTSs) graft in a rabbit model.
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Pulmonary arteriovenous malformations presenting as refractory heart failure.
J Thorac Dis
PUBLISHED: 02-17-2014
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A 22-year-old young man with a history of idiopathic dilated cardiomyopathy (IDC) was admitted to our hospital due to difficult-to-control heart failure. A thoracic X-ray showed multiple nodules at the both pulmonary hilus and upper lobe of the right lung. Computed tomography (CT) angiography of the thorax confirmed arteriovenous malformation (AVM). However, effective treatment was impossible due to the poor physical condition; he died a few days later. Here we reported on the case of pulmonary arteriovenous malformations (PAVMs) being misdiagnosed as refractory heart failure.
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Xylem formation can be modeled statistically as a function of primary growth and cambium activity.
New Phytol.
PUBLISHED: 01-26-2014
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Primary (budburst, foliage and shoot) growth and secondary (cambium and xylem) growth of plants play a vital role in sequestering atmospheric carbon. However, their potential relationships have never been mathematically quantified and the underlying physiological mechanisms are unclear. We monitored primary and secondary growth in Picea mariana and Abies balsamea on a weekly basis from 2010 to 2013 at four sites over an altitudinal gradient (25-900 m) in the eastern Canadian boreal forest. We determined the timings of onset and termination through the fitted functions and their first derivative. We quantified the potential relationships between primary growth and secondary growth using the mixed-effects model. We found that xylem formation of boreal conifers can be modeled as a function of cambium activity, bud phenology, and shoot and needle growth, as well as species- and site-specific factors. Our model reveals that there may be an optimal mechanism to simultaneously allocate the photosynthetic products and stored nonstructural carbon to growth of different organs at different times in the growing season. This mathematical link can bridge phenological modeling, forest ecosystem productivity and carbon cycle modeling, which will certainly contribute to an improved prediction of ecosystem productivity and carbon equilibrium.
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Association of miR-146a gene polymorphism with risk of nasopharyngeal carcinoma in the central-southern Chinese population.
J. Hum. Genet.
PUBLISHED: 01-16-2014
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This case-control study focused on estimating the association between miR-146a polymorphism and risk of nasopharyngeal carcinoma (NPC) in central-south China. In total, 160 patients with NPC and 200 healthy controls in central-south China were genotyped using polymerase chain reaction-restriction fragment length polymorphism assay. Chi-square test was used to assess the different distribution of miR-146a polymorphism between NPC patients and controls; and logistic regression analysis was applied to analyze the associations between miR-146a polymorphism with cancer risk in different contrast models. Significant differences between NPC patients and controls were found in genotype (P=0.033 for GG versus CG versus CC; and odds ratio (OR)=0.568, 95% confidence interval (CI)=0.354-0.912, P=0.019 for CG versus CC; and OR=0.503, 95% CI=0.261-0.971, P=0.041 for CG versus CC; and OR=0.564, 95% CI=0.360-0.884, P=0.012 for GG+CG versus CC, respectively) and allelic analysis (P=0.025 for G versus C). Our findings suggested that polymorphism of mir-146a was associated with NPC in the central-southern Chinese population.
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Checkpoint kinase 1 is negatively regulated by miR-497 in hepatocellular carcinoma.
Med. Oncol.
PUBLISHED: 01-15-2014
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Checkpoint kinase 1 (CHEK1) is an evolutionarily conserved Ser/Thr kinase, which mediates cell-cycle arrest after DNA damage, and we previously reported that CHEK1 was overexpressed and associated with poor prognosis in hepatocellular carcinoma (HCC), indicating it was oncogenic gene. In this study, we aimed to elucidate the mechanism of CHEK1 overexpression in HCC. We first verified the upregulated CHEK1 by qRT-PCR and western blot in 30 HCC samples compared with corresponding non-tumor liver tissues. In silico analysis showed that CHEK1 was a candidate target of miR-497, which was previously found to be downregulated in HCC by us. To test whether miR-497 could bind to 3'untranslated region (3'UTR) of CHEK1, luciferase reporter assay was conducted. The result revealed that miR-497 could bind to the 3'untranslated region (3'UTR) of CHEK1 mRNA. Western blot showed that ectopic expression of miR-497 suppressed the CHEK1 expression and inhibition of miR-497 led to significant upregulation of CHEK1. Finally, miR-497 expression was measured in the same 30 HCC samples, and the correlation between miR-497 and CHEK1 was analyzed. The results indicated that miR-497 was downregulated in HCC and had a significant negative correlation with CHEK1. Taken together, these results demonstrated that CHEK1 was negatively regulated by miR-497, and the overexpressed CHEK1 was resulted from the downregulated miR-497 in HCC, which provided a potential molecular target for HCC therapy.
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Coronary artery indexed diameter and z score regression equations in healthy Chinese Han children.
J Clin Ultrasound
PUBLISHED: 01-07-2014
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In children with coronary disease, clinical decision should be based on detailed measurements of the coronary arteries by two-dimensional echocardiography. We aimed to establish coronary artery reference indexed diameter and z scores regression equations in a large cohort of Chinese Han children.
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Measuring steady-state cerebral vasomotor reactivity using non-triggered phase-contrast magnetic resonance imaging.
Magn Reson Imaging
PUBLISHED: 01-03-2014
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To investigate cVMR by using CO2-based hypercapnic challenge and blood flow monitoring employing non-triggered phase contrast (PC) magnetic resonance imaging. Six healthy volunteers (6 male; mean age: 29 years) participated this study after providing institutionally approved consent. This study used non-triggered PC imaging to increase temporal resolution of dynamic blood flow measurements, allowing real-time monitoring of the hypercapnic challenge response. Results suggest that vasomotor reactivity measured by non-triggered PC imaging is positively associated with the concentration of inhaled CO2. This study concludes that CO2 challenge combined with non-triggered PC flow imaging is potentially useful to provide diagnostic information for patients with cerebrovascular disease.
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Cyclin D1 G870A Polymorphism and Risk of Nasopharyngeal Carcinoma: A Case-Control Study and Meta-Analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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Cyclin D1 (CCND1) plays a key role in cell cycle regulation. It is a well-established human oncogene which is frequently amplified or overexpressed in cancers. The association between CCND1 G870A polymorphism and cancer risk has been widely assessed. However, a definitive conclusion between CCND1 G870A polymorphism and risk of nasopharyngeal carcinoma (NPC) remains elusive.
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Izalpinin from fruits of alpinia oxyphylla with antagonistic activity against the rat bladder contractility.
Afr J Tradit Complement Altern Med
PUBLISHED: 01-01-2014
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Alpinia oxyphylla (Zingiberaceae), an herbaceous perennial plant, its capsular fruit is commonly used in traditional Chinese medicine for the treatment of different urinary incontinence symptoms including frequency, urgency and nocturia. These symptoms are similar to the overactive bladder syndrome. In our lab, we found that the 95% ethanol extract of the capsular fruits exhibited significant anti-muscarinic activity. Some constituents in capsular fruits including flavonoids (e.g., izalpinin and tectochrysin), diarylheptanoids (e.g., yakuchinone A and yakuchinone B) and sesquiterpenes (e.g., nootkatone), are regarded as representative chemicals with putative pharmacological activities.
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Correlation between exposure to magnetic fields and embryonic development in the first trimester.
PLoS ONE
PUBLISHED: 01-01-2014
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To explore the correlation between maternal magnetic field (MF) exposure in daily life and embryonic development.
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Combination of gefitinib and DNA methylation inhibitor decitabine exerts synergistic anti-cancer activity in colon cancer cells.
PLoS ONE
PUBLISHED: 01-01-2014
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Despite recent advances in the treatment of human colon cancer, the chemotherapy efficacy against colon cancer is still unsatisfactory. In the present study, effects of concomitant inhibition of the epidermal growth factor receptor (EGFR) and DNA methyltransferase were examined in human colon cancer cells. We demonstrated that decitabine (a DNA methyltransferase inhibitor) synergized with gefitinib (an EGFR inhibitor) to reduce cell viability and colony formation in SW1116 and LOVO cells. However, the combination of the two compounds displayed minimal toxicity to NCM460 cells, a normal human colon mucosal epithelial cell line. The combination was also more effective at inhibiting the AKT/mTOR/S6 kinase pathway. In addition, the combination of decitabine with gefitinib markedly inhibited colon cancer cell migration. Furthermore, gefitinib synergistically enhanced decitabine-induced cytotoxicity was primarily due to apoptosis as shown by Annexin V labeling that was attenuated by z-VAD-fmk, a pan caspase inhibitor. Concomitantly, cell apoptosis resulting from the co-treatment of gefitinib and decitabine was accompanied by induction of BAX, cleaved caspase 3 and cleaved PARP, along with reduction of Bcl-2 compared to treatment with either drug alone. Interestingly, combined treatment with these two drugs increased the expression of XIAP-associated factor 1 (XAF1) which play an important role in cell apoptosis. Moreover, small interfering RNA (siRNA) depletion of XAF1 significantly attenuated colon cancer cells apoptosis induced by the combination of the two drugs. Our findings suggested that gefitinib in combination with decitabine exerted enhanced cell apoptosis in colon cancer cells were involved in mitochondrial-mediated pathway and induction of XAF1 expression. In conclusion, based on the observations from our study, we suggested that the combined administration of these two drugs might be considered as a novel therapeutic regimen for treating colon cancer.
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[Advanced molecular technologies for the diagnosis of congenital malformation in neonates].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 11-16-2013
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Congenital malformation is one of the most frequent causes of infant death in western countries and major cities in China. Though genetic screening of newborns remains a hot issue and concern, the mortality rate associated with birth defects has not been significantly reduced over the past 20 years. Many genetic diseases manifest symptoms during the first 28 days of life, but full clinical symptoms might not be evident in newborns. Moreover, genetic aberrations is highly heterogeneous. These complicated factors lead to the establishment of diagnosis based on nonspecific or obscure symptoms. Recently developed array comparative genomic hybridization (CGH) and next generation sequencing (NGS) techniques with efficient high-resolution allow to screening of the entire genome for DNA copy number variants and sequencing respectively. These new and powerful tools can shorten the differential diagnosis process and quicken to movement towards targeted treatment and genetic and prognostic counseling.
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Assessment of 64-row computed tomographic angiography for diagnosis and pretreatment planning in pulmonary sequestration.
Radiol Med
PUBLISHED: 11-15-2013
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This study was done to evaluate the clinical implications and results of a prospective protocol using 64-row computed tomographic angiography (CTA) for diagnosis and pre-treatment planning in pulmonary sequestration (PS).
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[Clinical characteristics and imaging evaluation in children with renovascular hypertension].
Zhonghua Er Ke Za Zhi
PUBLISHED: 11-15-2013
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To characterize the clinical and angiographic features in children with renovascular hypertension.
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Sulfur-Containing Cytotoxic Curvularin Macrolides from Penicillium sumatrense MA-92, a Fungus Obtained from the Rhizosphere of the Mangrove Lumnitzera racemosa.
J. Nat. Prod.
PUBLISHED: 11-06-2013
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Sumalarins A-C (1-3), the new and rare examples of sulfur-containing curvularin derivatives, along with three known analogues (4-6), were isolated and identified from the cytotoxic extract of Penicillium sumatrense MA-92, a fungus obtained from the rhizosphere of the mangrove Lumnitzera racemosa . Their structures were established by detailed interpretation of NMR and MS data, and compound 1 was confirmed by X-ray crystallographic analysis. Compounds 1-3 and 5 showed potent cytotoxicity against some of the tested tumor cell lines. Sulfur substitution at C-11 or a double bond at C-10 significantly increased the cytotoxic activities of the curvularin analogues.
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Expression of Cx43-related microRNAs in patients with tetralogy of Fallot.
World J Pediatr
PUBLISHED: 10-21-2013
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Abnormal expression of connexin 43 (Cx43) has been reported to play an important role in the development of conotrunccal anomalies. However, less is known about the underlying reason for its abnormal expression. MicroRNAs (miRNAs), as an important part of gene expression regulation, have been implicated in some cardiac diseases. This study aimed to investigate the expression of Cx43 and its related miRNAs in patients with tetralogy of Fallot (TOF), and illustrate the potential role of abnormal miRNAs regulation to Cx43 expression in the pathology of TOF.
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[Comparative analysis of the clinical characteristics of orthopedic inpatients in Lushan and Wenchuan earthquakes].
Beijing Da Xue Xue Bao
PUBLISHED: 10-19-2013
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To systematically analyze and compare the clinical characteristics of orthopedic inpatients in Lushan and Wenchuan earthquake, so as to provide useful references for future earthquakes injury rescue.
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[Histone acetylation and expression of acetylation-related enzymes in children with tetralogy of Fallot].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 10-18-2013
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To study the expression of histone acetyltransferases (HATs) and histone deacetylases (HDACs) in children with tetralogy of Fallot (TOF), and to investigate the role of histone acetylation and acetylation-related enzymes in the pathogenesis of TOF.
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Preparation of N-doped TiO2 by oxidizing TiN and its application on phenol degradation.
Water Sci. Technol.
PUBLISHED: 08-30-2013
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Incomplete oxidation of titanium nitride (TiN) to prepare nitrogen-doped TiO2 was verified by calcining TiN at different temperatures in air for 30 min. The as-prepared samples were characterized by X-ray diffraction, UV-Vis diffuse reflectance spectra and X-ray photoelectron spectroscopy. The results confirmed that oxidizing TiN incompletely is an effective and simple method to prepare nitrogen-doped TiO2. Photocatalytic degradation of phenol was conducted to evaluate the photocatalytic activity of as-prepared samples. The results showed that phenol can be degraded efficiently by the as-prepared samples under visible light; low phenol concentration was conducive to degradation; the optimum calcination temperature and photocatalyst dosage are 650 °C and 0.5 g/L, respectively. The effects of different light sources on phenol degradation were compared. The reusability of nitrogen-doped TiO2 was tested and the results indicated a relatively good reusability under laboratory conditions.
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[Effect of fertilization on phenolic components and antioxidant activities of Artemisia annua].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 08-17-2013
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A pot experiment with variable fertilizer treatments was carried out to study the influence of fertilization on the concentration and accumulation of polyphenols, scopoletin, chrysosplenol-D and chrysosplenetin in roots, stems and leaves and their antioxidant activities. The main aims were to fertilize scientifically in cultivation of Artemisia annua and improve the quality of the harvest organs.
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[Changes in phototaxical index of Daphnia carinata under electric field of direct current in response to Cr6+ and Hg2+].
Huan Jing Ke Xue
PUBLISHED: 08-17-2013
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In order to detect toxicants sensitively in water by phototaxity of Daphnia, we studied the influence of DC electric field on phototaxity of self-bred Daphnia carinata, a mono-clone with high phototaxity, and the changes of their phototaxity in response to Cr6+ and Hg2+ after the treatment of DC electric field. The result indicated that the treatment of DC electric field changed their phototaxical indexes from positive to negative as the electric density increased and the time prolonged. The criteria values that showed no sign of changes in the phototaxical index of Daphnia carinata were 0.01 mA for 5 min. However, the limit for detecting Cr6+ and Hg2+ by their phototaxical index decreased significantly after treatment with 0.01 mA/5 min. The detection limits were only 32 microg x L(-1) for Cr6+ and 4.2 microg x L(-1) for Hg2+ after this treatment, much lower than those without pretreatment (56 microg x L(-1) for Cr6+ and 5.6 microg x L(-1) for Hg2+, respectively). Therefore, appropriate DC electric field to pre-treat Daphnia could decrease the detection limit, widen the concentration range of detection, and increased the sensibilities in water monitoring.
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[Allelopathic effect of artemisinin on green algae].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 08-16-2013
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To study the growth effects of differing concentrations of artemisinin on green algae and to evaluate the ecological risk. The effects of artemisinin on the growth and the content change of chlorophyll, protein, oxygen, conductivity, SOD, CAT, MDA in Chlorella pyrenoidosa and Scenedesmus oblique were studied through 96 h toxicity tests. Artemisinin accelerated the growth of algae at a lower concentration ( <40 microg . L-1) with content increase of chlorophyll or protein and so on, and it inhibited the growth of algae at higher concentration ( >80 microg . L-1). The content of chlorophyll or protein in algae cells reduced with the increasing concentration of artemisinin, exhibiting the good concentration-effect relationship. SOD and CAT activity was stimulated at low concentrations ( <40 microg . L-1 ) and inhibited at high concentrations ( >80 microg . L- 1). However, MDA content increased significantly with the increase of concentration. According to the seven kinds of indicators changes, the time-response and dose-response suggested that the surfactant first hurt in Ch. pyrenoidosa was damaging membrane by changing membrane lipid molecules soluble. And primary mechanism on Chlorophyta cells might be related to the oxidation damage of lipid and other biological large molecules caused by artemisinin. The large-scale intensive planting of Artemisia annua may reduce the surrounding water productivity.
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Neonatal pulse oximetry screening improves detecting of critical congenital heart disease.
Chin. Med. J.
PUBLISHED: 07-24-2013
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Life-threatening critical congenital heart disease (cCHD) is often not detected in the neonatal period. Unrecognized cCHD results in high morbidity and mortality rates. As a non-invasive, convenient, quick and accurate measuring method, pulse oximetry is considered to be a promising strategy to screen for cCHD in neonates. This article is a review on the neonatal pulse oximetry screening for cCHD.
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Factors influencing discomfort during anterior ultrasound-guided injection for hip arthrography.
J Chin Med Assoc
PUBLISHED: 07-09-2013
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Although ultrasound (US)-guided injection techniques for magnetic resonance arthrography of the hip have been used with increasing frequency to diagnose internal joint derangements, little is known about patient tolerance, which is relevant information for patients. The objective of this study was to evaluate prospectively the association between possible influencing factors and discomfort felt during the performance of anterior US-guided injection techniques targeting the femoral head-neck junction during hip arthrography.
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Docetaxel (Taxotere®)-induced cavitary change of pulmonary metastatic lesions complicated by bilateral spontaneous pneumothoraces in a patient with primary adenocarcinoma of the lung.
Singapore Med J
PUBLISHED: 07-04-2013
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Pneumothorax is a complication that rarely occurs after chemotherapy for lung cancer. We report the chest computed tomography findings of a case of spontaneous pneumothorax complicating docetaxel (Taxotere®) treatment for pulmonary metastasis in a 70-year-old woman with pulmonary adenocarcinoma. The patient developed bilateral pneumothoraces, which was induced by changes in the cavitary pulmonary metastatic lesions, after systemic chemotherapy with docetaxel. The chest computed tomography findings and possible mechanisms of this unusual complication are discussed in this report.
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The thickness of the anterior compartment does not indicate compartment syndrome in acutely traumatised legs?
Injury
PUBLISHED: 06-29-2013
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The study was conducted to achieve early detection of increased compartment pressure by comparing the changes between the thickness and pressure within the musculofascial compartments after lower leg injury.
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Clinical significance and prognostic value of microRNA expression signatures in hepatocellular carcinoma.
Clin. Cancer Res.
PUBLISHED: 06-28-2013
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MicroRNAs (miRNAs) play important roles in the development and progression of cancer. The aim of this study is to identify miRNA expression signatures in hepatocellular carcinoma and delineate their clinical significance for hepatocellular carcinoma.
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Genetic variants reducing MTR gene expression increase the risk of congenital heart disease in Han Chinese populations.
Eur. Heart J.
PUBLISHED: 06-24-2013
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Elevated homocysteine levels are known to be a risk factor for congenital heart disease (CHD), but the mechanism underlying this effect is unknown. During early embryonic development, homocysteine removal is dictated exclusively by the MTR activity. To examine the role of MTR in CHD risk, we identified genetic variants in MTR and investigated the mechanisms that affect its expression levels and that increase the risk of CHD in Chinese populations.
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Effects of microvascular permeability changes on contrast-enhanced T1 and pharmacokinetic MR imagings after ischemia.
Stroke
PUBLISHED: 06-06-2013
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Brain enhancement on contrast-enhanced T1-weighted imaging (CET1-WI) after ischemic stroke is generally accepted as an indicator of the blood-brain barrier disruption. However, this phenomenon usually starts to become visible at the subacute phase. The purpose of this study was to evaluate the time-course profiles of K(trans), cerebral blood volume (vp), and CET1-WI with early detection of blood-brain barrier changes on K(trans) maps and their role for prediction of subsequent hemorrhagic transformation in acute middle cerebral arterial infarct.
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[Allelopathic effects of extracts from fibrous roots of Coptis chinensis on two leguminous species].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 05-31-2013
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An experiment was carried out to study the allelopathic effects of Coptis chinensis fibrous root extracts (CRE) on the germination and seedling growth of Vicia faba and Pisum sativum in order to alleviate the allelopathic effects and increase land productivity. The seeds of both garden pea (P. sativum) and broad been (V. faba) were germinated in CRE solution of various concentrations, the germination rate, seedling growth and related physiological indexes were measured. The result indicated that there were no significant effects of CRE in low concentrations on seed germination, including both the rate and index, and seed vitality and membrane permeability. With the increment of CRE concentrations, however, the high seed membrane permeability and germination inhibition were observed. For example, the germination rates were reduced by 23.4% (P. sativum) and 9.5% (V. faba), respectively, in CRE solution with 800 mg . L-1. Simultaneously, soluble sugars and the free amino acids in the seeds were lower than those in the control (without CRE) after soaking seeds in CRE solutions. In addition, the seedling growth and nitrate reductase activity were stimulated by CRE at low concentrations in contrast to high concentrations which behaved otherwise and inhibited the nutrient utilization in endosperm. Therefore, the large amount of allelochemicals released from the roots and remains of C. chinensis in soils could inhibit the seed germination and seedling growth of legumes, which may lead to decrease even fail crop yields after growing this medical plant.
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Orphan nuclear receptor nurr1 as a potential novel marker for progression in human prostate cancer.
Asian Pac. J. Cancer Prev.
PUBLISHED: 05-18-2013
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A number of studies have indicated that Nurr1, which belongs to a novel class of orphan nuclear receptors (the NR4A family), is important for carcinogenesis. Here we investigated expression of Nurr1 protein in benign and malignant human prostate tissues and association with clinicopathologic features using immunohistochemical techniques. Moreover, we also investigated the ability of Nurr1 to influence proliferation, migration, invasion and apoptosis of human prostate cancer cells using small interfering RNA silencing. Immunohistochemical analysis revealed that the expression of Nurr1 protein was higher in prostate cancer tissues than in benign prostate tissue (P < 0.001), levels being positively correlated with tumor T classification (P = 0.003), N classification (P = 0.017), M classification (P = 0.011) and the Gleason score (P = 0.020) of prostate cancer patients. In vitro, silencing of endogenous Nurr1 attenuated cell proliferation, migration and invasion, and induced apoptosis of prostate cancer cells. These results suggest that Nurr1 may be used as an indicator for prostate cancer progression and be useful for novel potential therapeutic strategies.
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Inhibition of NADPH oxidase 4-related signaling by sodium hydrosulfide attenuates myocardial fibrotic response.
Int. J. Cardiol.
PUBLISHED: 04-29-2013
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Myocardial fibrosis plays a pivotal role in the development of heart failure. Hydrogen sulfide (H2S) is an endogenous gasotransmitter with potent cardioprotective properties; however, whether H2S is involved in fibrotic process remains unknown. This study aimed to explore the role of H2S in the process of cardiac fibrosis and the underlying mechanisms.
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MicroRNA Deregulation in Right Ventricular Outflow Tract Myocardium in Nonsyndromic Tetralogy of Fallot.
Can J Cardiol
PUBLISHED: 04-28-2013
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Tetralogy of Fallot (TOF) is 1 of the most common heart defects in children, and the underlying mechanisms remain largely elusive. MicroRNAs (miRNAs) are a class of regulators of gene expression and are increasingly recognized for their roles in heart development.
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Effects of steroid hormones on reproduction- and detoxification-related gene expression in adult male mosquitofish, Gambusia affinis.
Comp. Biochem. Physiol. C Toxicol. Pharmacol.
PUBLISHED: 03-24-2013
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The molecular mechanisms that mediate fish reproduction and detoxification in response to steroid hormones were studied by using adult male western mosquitofish (Gambusia affinis) as sentinel species. The expression patterns of three vitellogenins (VtgA, VtgB and VtgC), two estrogen receptors (ER? and ER?), two androgen receptors (AR? and AR?), metallothionein (MT) and cytochrome P450 1A (CYP1A) in the liver and testis of adult male mosquitofish were assessed through exposure treatments with progesterone (P), testosterone (T) and 17?-estradiol (E2), alone and in combination for eight days. The results showed that expression patterns of Vtg subtype, ER subtype, AR subtype, MT and CYP1A genes in male mosquitofish varied according to tissue and specific hormone stress. Vtg subtype mRNA expression was induced in the liver in E2-added treatments, and an up-regulation of ER? mRNA expression was also observed. In addition, hormone treatments increased three Vtg subtype mRNA expression levels in the testis, at least to some extent. All hormone treatments significantly inhibited ER?, ER? and AR? mRNA expression in the testis. Some of hormone treatments could affect MT and CYP1A gene expression in mosquitofish. In general, multiple hormone treatments showed different effects on target gene expression compared with corresponding hormone alone. The results from the present study provided valuable information on the toxicological effects of steroid hormones in mosquitofish.
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Prolyl isomerase Pin1 regulated signaling pathway revealed by Pin1 +/+ and Pin1 -/- mouse embryonic fibroblast cells.
Pathol. Oncol. Res.
PUBLISHED: 03-22-2013
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Pin1 (peptidylprolyl cis/trans isomerase, NIMA-interacting 1) plays a key role in a number of diseases including cancer and Alzheimer disease. Previous studies have identified a wide range of phosphoproteins as Pin1 substrates. Related pathways were analyzed separately. The aim of this study was to provide a comprehensive picture involving Pin1 regulation. A genome-wide mRNA expression microarray was carried out using the RNA isolation from Pin1 (+/+) and Pin1 (-/-) mouse embryonic fibroblast (MEF) cells. Signaling pathways regulated by Pin1 were analyzed with the utility of KEGG pathway and GO annotation. An expression pattern regulated by Pin1 was revealed. A total of 606 genes, 375 being up-regulated and 231 down-regulated, were differentially expressed when comparing Pin1 +/+ to Pin1 -/- MEF cells. Totally 48 pathways were shown to be regulated by Pin1 expression in KEGG pathway analysis. In the GO annotation system, 19 processes on biological processes, 15 processes on cellular components, and 18 processes on molecular functions were found to be in the regulation of Pin1 expression. Pathways related to immune system and cancer showed most significant association with Pin1 regulation. Pin1 is an important regulator in a wide range of signaling pathways that were related to immune system and cancer.
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[Influence of aluminum and manganese on the growth, nutrient uptake and the efflux by ectomycorrhizal fungi].
Huan Jing Ke Xue
PUBLISHED: 03-16-2013
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Al3+ and Mn2+ limit forest growth and vegetation restoration in strongly acidic soils and mining areas of aluminum and manganese. The knowledge on the influence of these two elements on ectomycorrhizal fungi can provide theoretical and technical supports for the selection of powerful ectomycorrhizal fungal strains and the bioremediation of contaminated soil. Three ectomycorrhizal fungal strains, namely Suillus luteus 13 (Sl 13), Cenococcum geophilum 04 (Cg 04) and Pisolithus tinctorius 715 (Pt 715), were grown in liquid culture mediums with Al3+ and Mn2+ added alone and together to investigate fungal growth, nutrient uptake and organic acid efflux. The results showed that the biomass of Sl 13, Cg 04 and Pt 715 was decreased by 70.35%, 52.44% and 18.55%, respectively, under Mn2+ stress. Al3 also decreased the biomass of Sl 13 by 50.74% but increased that of Cg 04. The growth of ectomycorrhizal fungi was further inhibited when grown in culture solutions with addition of both Mn2+ and Al3 and the least growth inhibition was found with Pt 715. Cg 04 might thus have a strong resistance to Al3+ stress and Pt 715 to both Al3+ and Mn2+ compared to the others. Al3+ and Mn2+ decreased the nutrient uptake by the fungi, particularly by Sl 13 which showed more obvious reduction than Pt 715 and Cg 04. However, Al3+ and Mn2+ increased the efflux of oxalic acid and protons by ectomycorrhizal fungi. An additional oxalic acid exudation by Cg 04 was observed in the coexistence of Al3+ and Mn2+ and Pt 715 exuded not only oxalic acid but also succinic acid. Therefore, ectomycorrhizal fungi resistant to Mn2+ and Al3+ could effuse more organic acids than the sensitive ones in order to alleviate the harmfulness through complexation under the stress.
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The application of MR imaging in the detection of hip involvement in patients with ankylosing spondylitis.
Eur J Radiol
PUBLISHED: 03-11-2013
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To investigate the changes in hip MR imaging, evaluate the frequency of hip involvement and compare the value of clinical symptoms, radiographs, and MR imaging in the detection of hip involvement in patients with ankylosing spondylitis (AS).
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Expression of NF-?B and osteopontin of synovial fluid of patients with knee osteoarthritis.
Asian Pac J Trop Med
PUBLISHED: 03-10-2013
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To explore the significance of osteopontin and nuclear factor ?B (NF-?B) expression in patients with knee osteoarthritis.
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Dual-delivery of vancomycin and icariin from an injectable calcium phosphate cement-release system for controlling infection and improving bone healing.
Mol Med Rep
PUBLISHED: 03-08-2013
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Infectious bone diseases following severely contaminated open fractures are frequently encountered in clinical practice. It is difficult to successfully repair bone and control infection at the same time. To identify a better treatment method, we prepared a dual-drug release system that was comprised of icariin (IC, a natural osteoinductive molecule), vancomycin (VA) and injectable calcium phosphate cement (CPC). The ultrastructure of the dual-drug release system was evaluated by scanning electron microscopy and the biocompatibility was assessed by cell culture. In addition, the release kinetics of IC and VA were respectively investigated by using high?performance liquid chromatography. Finally, this system was used to repair Staphylococcus aureus-contaminated bone defects in a rabbit model. Twelve weeks after the implantation of IC-VA/CPC, the contaminated bone defects were completely repaired, with significantly improved formation of lamellar bone and recanalization of the marrow cavity compared with the controls (CPC without antibiotics or osteoinductive agent). These results demonstrate that this dual-drug release system, with its concomitant antibiotic and osteoinductive properties, has significant potential for the treatment of contaminated bone injury or infectious bone disease.
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Hormonal effects of tetrabromobisphenol A using a combination of in vitro and in vivo assays.
Comp. Biochem. Physiol. C Toxicol. Pharmacol.
PUBLISHED: 03-04-2013
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The aim of this study was to investigate the hormonal effects of tetrabromobisphenol A (TBBPA) in vitro on recombinant yeasts and in vivo on mosquitofish (Gambusia affinis). The in vitro bioassays for (anti-)androgenic activities showed that TBBPA had a weak androgenic activity in vitro with recombinant yeast systems carrying human androgen receptor (hAR). In the in vivo bioassays, the gene expression patterns of vitellogenin (Vtg), estrogen receptors (ER? and ER?), and androgen receptors (AR? and AR?) in adult males and juveniles after exposure to TBBPA for 60 days were evaluated. Significant up-regulation of Vtg, ER?, and ER? mRNAs was observed in the liver after exposure to 500 nM of TBBPA. In the testis, the lowest concentration of TBBPA (50 nM) markedly induced Vtg, ER?, and AR? mRNA expression, but the same concentration significantly inhibited AR? mRNA expression. In addition, in juveniles, 100 nM of TBBPA significantly up-regulated the expression of Vtg, ER?, and AR? mRNAs. However, TBPPA did not cause histological alterations in the liver and testis of adult male mosquitofish. The results from this present study suggest that TBBPA could display low but multiple hormonal activities despite its low toxicity to mosquitofish.
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Outcomes of thrombolysis with and without predilation of the inferior vena cava (IVC) in patients with Budd-Chiari syndrome with old IVC thrombosis.
Vasc Endovascular Surg
PUBLISHED: 02-14-2013
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To compare the efficacy of thrombolysis with and without predilation of the inferior vena cava (IVC) for Budd-Chiari syndrome (BCS) with old IVC thrombosis.
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Management of limb fractures in a teaching hospital: comparison between Wenchuan and Yushu earthquakes.
Chin. J. Traumatol.
PUBLISHED: 02-07-2013
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To comparatively analyze the medical records of patients with limb fractures as well as rescue strategy in Wenchuan and Yushu earthquakes so as to provide references for post-earthquake rescue.
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Infectious complications in patients with crush syndrome following the Wenchuan earthquake.
Chin. J. Traumatol.
PUBLISHED: 02-07-2013
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To analyze the results of clinical and bacteriological examinations in patients with crush syndrome who suffered infectious complications after an earthquake in Sichuan, China.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.