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Find video protocols related to scientific articles indexed in Pubmed.
Measurement of slurry droplets in coal-fired flue gas after WFGD.
Environ Geochem Health
PUBLISHED: 06-05-2014
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China owns the world's largest capacity of coal-fired power units. By the end of 2012, the capacity of installed national thermal power has been 819.68 million kilowatts. The latest standard requires that newly built power plants emit SO2 in no more than 100 mg/m(3) and the emission of old ones be lower than 200 mg/m(3) while in some key areas the emission should be controlled under 50 mg/m(3). So by the end of 2012, 90 % of the active coal-fired units have been equipped with flue gas desulfurization devices. Among the desulfurization methods adopted, limestone-gypsum wet flue gas desulphurization accounts for 92 %, causing the problem of fine droplets in the exhaust gas after defogger, which may even form "gypsum rain." At present, sampling methods are widely used at home and abroad, such as magnesium ion tracer method, modified magnesium ion tracer method and chemical analysis. In addition, some scholars use aerodynamic methods, such as ELPI, to measure the diameter distribution and concentration. The methods mentioned above all have their own demerits, such as the inability to on-line, continuous measurements and the need of prolonged measuring time. Thus, in this paper some potential optical on-line methods are presented, such as Fraunhofer diffraction pattern analysis and wavelength-multiplexed laser extinction. Also brought up are their measuring scope and merits. These methods have already been utilized to measure small liquid droplets and their demonstrations and evaluations are as well stated. Finally, a 3D imaging method based on digital holographic microscope is proposed for in-line measurement of size and concentration of slurry droplets. The feasibility of this method is demonstrated by preliminary experimental investigation.
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Inhibition of endometrial cancer by n-3 polyunsaturated fatty acids in preclinical models.
Cancer Prev Res (Phila)
PUBLISHED: 05-27-2014
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Although preclinical and epidemiologic studies have shown the importance of n-3 polyunsaturated fatty acids (PUFA) in the prevention of hormone-responsive cancers such as breast cancer, evidence of the association between n-3 PUFAs and endometrial cancer risk is limited and no previous study has examined the effect of n-3 PUFAs on endometrial cancer in cellular and animal models. In this study, we demonstrated that docosahexenoic acid (DHA) dose- and time-dependently inhibited endometrial cancer cell proliferation, colony formation, and migration and promoted apoptosis. Dietary n-3 PUFAs efficiently prevented endometrial cancer cell growth in xenograft models. Moreover, ectopic expression of fat-1, a desaturase, catalyzed the conversion of n-6 to n-3 PUFAs and produced n-3 PUFAs endogenously, also suppressed endometrial tumor cell growth and migration, and potentiated apoptosis in endometrial cancer cell lines. Interestingly, implanted endometrial cancer cells were unable to grow in fat-1 transgenic SCID mice. Further study revealed that mTOR signaling, which plays an essential role in cell proliferation and endometrial tumorigenesis, is a target of n-3 PUFAs. Exogenous or endogenous n-3 PUFAs efficiently suppressed both mTOR complex 1 (mTORC1) and mTORC2 in vitro and in vivo. Moreover, both dietary n-3 PUFAs and transgenic expression of fat-1 in mice effectively repressed mTORC1/2 signaling and endometrial growth elicited by unopposed estrogen. Taken together, our findings provide comprehensive preclinical evidences that n-3 PUFAs efficiently prevent endometrial cancer and establish mTORC1/2 as a target of n-3 PUFAs.
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Organocatalytic enantioselective construction of multi-functionalized spiro oxindole dienes.
Org. Biomol. Chem.
PUBLISHED: 05-21-2014
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A diastereo- and enantio-selective domino Michael-cyclization-tautomerization reaction of isatylidene malononitriles with ?,?-dicyanoalkenes catalyzed by a cinchona alkaloid-derived bifunctional thiourea catalyst has been developed. A series of multi-functionalized spiro oxindole diene derivatives have been obtained in good to excellent yields (up to 97%) with good to excellent enantioselectivities (up to 96%) as well as good diastereoselectivities (up to 7.9?:?1). In addition, an anomalous temperature effect on the enantioselectivity has also been studied for this transformation.
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TGF?R2 is a major target of miR-93 in nasopharyngeal carcinoma aggressiveness.
Mol. Cancer
PUBLISHED: 03-01-2014
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MiR-17-92 cluster and its paralogues have emerged as crucial regulators of many oncogenes and tumor suppressors. Transforming growth factor-? receptor II (TGF?R2), as an important tumor suppressor, is involved in various cancer types. However, it is in cancer that only two miRNAs of this cluster and its paralogues have been reported so far to regulate TGF?R2. MiR-93 is oncogenic, but its targetome in cancer has not been fully defined. The role of miR-93 in nasopharyngeal carcinoma (NPC) still remains largely unknown.
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Increased expression of cathepsin L: a novel independent prognostic marker of worse outcome in hepatocellular carcinoma patients.
PLoS ONE
PUBLISHED: 01-01-2014
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To investigate the expression and role of Cathepsin L (CTSL) in Hepatocellular carcinoma (HCC) tissue and cell line (MHCC-97H), and to evaluate the clinical and prognostic significance of CTSL protein in patients with HCC.
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Artesunate inhibits the growth of gastric cancer cells through the mechanism of promoting oncosis both in vitro and in vivo.
Anticancer Drugs
PUBLISHED: 08-21-2013
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This study aims to investigate the significance and mechanism of artesunate involved in suppressing the proliferation of gastric cancer in vitro and in vivo. In the in-vitro experiments, artesunate inhibited the growth of gastric cancer cell lines (SGC-7901, BGC-823, and AGS) with concentration-dependent activity, with no significant effect on GES-1 cells. BGC-823 cells treated with artesunate showed the typical morphologic features of oncosis rather than apoptosis. Meanwhile, we observed calcium overload, downregulation of vascular endothelial growth factor expression, and upregulation of calpain-2 expression in the artesunate-treated BGC-823 cells. In addition, the in-vivo study showed that artesunate produced a dose-dependent tumor regression in nude mice. The antitumor activity of 240 mg/kg artesunate was similar to that of 10 mg/kg docetaxel. Furthermore, compared with the control group, no significant difference was observed in the body weight of artesunate-treated nude mice other than docetaxel-treated nude mice. These observations show that artesunate has concentration-dependent inhibitory activities against gastric cancer in vitro and in vivo by promoting cell oncosis through an impact of calcium, vascular endothelial growth factor, and calpain-2 expression.
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Quantum critical point of spin-boson model and infrared catastrophe in bosonic bath.
J Chem Phys
PUBLISHED: 05-10-2013
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An analytic ground state is proposed for the unbiased spin-boson Hamiltonian, which is non-Gaussian and beyond the Silbey-Harris ground state with lower ground state energy. The infrared catastrophe in Ohmic and sub-Ohmic bosonic bath plays an important role in determining the degeneracy of the ground state. We show that the infrared divergence associated with the displacement of the nonadiabatic modes in bath may be removed from the proposed ground state for the coupling ? < ?c. Then ?c is the quantum critical point of a transition from non-degenerate to degenerate ground state and our calculated ?c agrees with previous numerical results.
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Secondary structure prediction for complete rDNA sequences (18S, 5.8S, and 28S rDNA) of Demodex folliculorum, and comparison of divergent domains structures across Acari.
Exp. Parasitol.
PUBLISHED: 05-06-2013
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According to base pairing, the rRNA folds into corresponding secondary structures, which contain additional phylogenetic information. On the basis of sequencing for complete rDNA sequences (18S, ITS1, 5.8S, ITS2 and 28S rDNA) of Demodex, we predicted the secondary structure of the complete rDNA sequence (18S, 5.8S, and 28S rDNA) of Demodex folliculorum, which was in concordance with that of the main arthropod lineages in past studies. And together with the sequence data from GenBank, we also predicted the secondary structures of divergent domains in SSU rRNA of 51 species and in LSU rRNA of 43 species from four superfamilies in Acari (Cheyletoidea, Tetranychoidea, Analgoidea and Ixodoidea). The multiple alignment among the four superfamilies in Acari showed that, insertions from Tetranychoidea SSU rRNA formed two newly proposed helixes, and helix c3-2b of LSU rRNA was absent in Demodex (Cheyletoidea) taxa. Generally speaking, LSU rRNA presented more remarkable differences than SSU rRNA did, mainly in D2, D3, D5, D7a, D7b, D8 and D10.
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Heparanase is involved in the proliferation and invasion of nasopharyngeal carcinoma cells.
Oncol. Rep.
PUBLISHED: 03-05-2013
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Heparanase (HPSE), an endo-?-D-glucuronidase, is overexpressed in nasopharyngeal carcinoma (NPC). The purpose of our study was to investigate the possible role of HPSE in the development of NPC. RNA interference (RNAi) using an HPSE small hairpin RNA (HPSE shRNA) was used to identify the effects of HPSE on the regulation of the malignant behaviors of NPC. CNE-2, a highly metastatic human NPC cell line in which HPSE mRNA and protein levels were detected to be the highest in three NPC cell lines involved in the research, was selected as a cell model in vitro and in vivo. The results showed that downregulation of HPSE significantly inhibited the proliferative and invasive abilities of CNE-2 cells partially through MAPK signaling. Compared with the parental NPC cells, HPSE-silenced cells exhibited attenuated capacity for developing tumors in nude mice, while the growth of tumor xenografts derived from these cells was dramatically suppressed. In conclusion, our results suggest that HPSE contributes to the proliferation and metastasis of NPC, and HPSE may be a potent molecular target for NPC treatment.
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The protective effect of Cold-inducible RNA-binding protein (CIRP) on testicular torsion/detorsion: an experimental study in mice.
J. Pediatr. Surg.
PUBLISHED: 02-19-2013
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To evaluate the expression of Cold-inducible RNA-binding protein (CIRP) in torsion/detorsion of the testes in different phases and demonstrate the protective effect of CIRP on testicular injury after torsion/detorsion (T/D) in an experimental mouse model.
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Thymosin alpha-1-transformed Bifidobacterium promotes T cell proliferation and maturation in mice by oral administration.
Int. Immunopharmacol.
PUBLISHED: 01-24-2013
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Thymosin alpha-1 (T?1) has been used as an immune potentiator for treatment of immune deficiency diseases by injection administration. However, injection is inconvenient and may cause many side effects. In order to improve the administration convenience of T?1, a human T?1 gene transformed Bifidobacterium longum (BL-T?1) was prepared and its effects on mice immunity by oral administration were investigated. The Balb/c mice were treated with BL-T?1, which was pre-induced with 0.2% l-arabinose, every other day for 2 weeks. The B. longum transformed with empty vector (BL-0) was used as the negative control, and normal saline (NS, 0.9% saline) was used as the blank control. The results shown that (1) the CD3(+)CD4(+) and CD3(+)CD8(+) T-cells in blood, spleen and thymus, and the CD4(+)CD8(+) cells in thymus and spleen of BL-T?1 group were all significantly increased than that of negative control BL-0 group respectively; (2) the interferon-? (IFN-?) and interleukin-12 (IL-12) in serum of BL-T?1 group were significantly increased. No significant differences were found in the levels of tumor necrosis factor-? (TNF-?) and interleukin-4 (IL-4) between BL-T?1 group and BL-0 group; (3) thymic hyperplasia and lymphadenectasis were observed in BL-T?1 group after three-month treatment. In conclusion, the T?1-transformed B. longum promotes thymus and lymph nodes growth, stimulates T cell proliferation and maturation, and enhances cellular immunity through Th1 pathway by oral administration.
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Entanglement evolution and quantum phase transition of biased s = 1/2 spin-boson model.
Phys Rev E Stat Nonlin Soft Matter Phys
PUBLISHED: 02-28-2011
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The ground state and the spectral structure of lower-lying excited states of a dissipative two-level system coupled to a sub-Ohmic bath (s = 1/2) with nonzero bias have been studied using the unitary transformation method. By calculating the ground-state entanglement entropy, the ground-state average of ?(z)(G), and the static susceptibility of the two-level system, we explore the nature of the transition (crossover) between the delocalized and localized state of the two-level system. Furthermore, we calculate the time-dependent expectation (?(z)(t)) and the time evolution of the entanglement entropy to show that, when the system undergoes a transition (crossover) from the delocalized to the localized state, the time evolution of the two-level system changes from coherent to decoherent dynamics.
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[Inhibitory effect of photodynamic therapy combined with paclitaxel on the proliferation of esophageal carcinoma Eca-109 cells].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 06-30-2010
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To investigate the inhibitory effect of photodynamic therapy (PDT) in combination with paclitaxel (PCT) on proliferation in esophageal carcinoma Eca-109 cells line.
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[Expression of PAK4 in breast cancer and benign breast pathological changes].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 05-27-2010
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To study the role of p21-activated kinase-4 (PAK4) in the occurrence, progression and metastasis of breast cancer.
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[Inhibitory effect of ZD6474 combined with adriamycin on MCF-7 human breast cancer cells in vitro].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 03-26-2010
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To investigate the killing effect of ZD6474 combined with adriamycin (ADM) on MCF-7 human breast cancer cells.
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Non-markovian dynamics of a biased qubit coupled to a structured bath.
J Phys Condens Matter
PUBLISHED: 02-23-2010
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A new analytical approach, beyond the rotating wave approximation, based on unitary transformations and the non-markovian master equation for the density operator, is applied to treat the biased spin-boson model with a Lorentzian structured bath for arbitrary detunings at zero temperature. Compared to zero bias, we find that the non-equilibrium dynamics demonstrates two more damping oscillation frequencies and one additional relaxation frequency for non-zero bias. Analytical expressions for the non-markovian dynamics and the corresponding spectrum, the localized-delocalized transition point, the coherent-incoherent transition point, the analytical ground energy, the renormalized tunneling factor and the susceptibility are determined. The sum rule and the Shiba relation are checked in the coherent regime.
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Ras homologue enriched in brain is a critical target of farnesyltransferase inhibitors in non-small cell lung cancer cells.
Cancer Lett.
PUBLISHED: 01-18-2010
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The purpose of this work is to study mechanisms underlying anti-tumor effects of farnesyltransferase inhibitors (FTIs) in non-small cell lung cancer (NSCLC). We demonstrate that mRNA and protein levels of Ras homologue enriched in brain (Rheb) are highly expressed both in NSCLC tissues and in NSCLC cell lines. Rheb expression levels correlate with phosphorylation of its downstream target S6 and the sensitivity of NSCLC cells to FTIs (R115777 and SCH66336)-induced growth inhibition and apoptosis. FTIs effectively and preferentially inhibited Rheb downstream signaling in NSCLC cells. Moreover, inhibition of Rheb functions by FTIs or dominant-negative Rheb mutants enhance the effects of cisplatin on NSCLC cells. Rheb-CSVL, a FTIs-resistant mutant, reduces the effects of FTIs on NSCLC cells. Our results suggest that Rheb is a critical target for FTIs therapy in NSCLC.
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Influence of temperature on coherent dynamics of a two-level system immersed in a dissipative spin bath.
J Chem Phys
PUBLISHED: 10-10-2009
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The quantum dynamics of a two-level system coupled to an Ohmic spin bath is studied by means of the perturbation approach based on a unitary transformation. A scattering function xi(k) is introduced in the transformation to take into account quantum fluctuations. By the master equation within the Born approximation, nonequilibrium dynamics quantities are calculated. The method works well for the coupling constant 0
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[Clinical research of bevacizumab in combination with irinotecan, fluorouracil and leucovorin for advanced metastatic colorectal cancer].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 07-15-2009
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To evaluate the efficacy of bevacizumab in combination of irinotecan,fluorouracil and leucovorin for metastatic colorectal cancer treated by failed prior oxaliplatin -based regiment.
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[The effect of diethylstilbestrol on inducing abdominal cryptorchidism and relevant genetic expression in rats].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 06-19-2009
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To study the effect of diethylstilbestrol (DES) at different doses on transabdominal testicular descent in rats and the expression of INSL3 in the testis and HOXA10 in the gubernaculum.
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Prenatal exposure to diaethylstilbestrol in the rat inhibits transabdominal testicular descent with involvement of the INSL3/LGR8 system and HOXA10.
Chin. Med. J.
PUBLISHED: 06-05-2009
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Prenatal exposure to diaethylstilbestrol (DES) has been found to lead to intra-abdominal cryptorchidism, but the mechanism is still not completely clear. This study investigated the roles of the INSL3/LGR8 system and HOXA10 in DES-induced intra-abdominal cryptorchidism (DIIAC). The effect of DES on steroidogenic factor-1 (SF-1), that has been reported to control transcription of insulin-like factor 3 (INSL3), was also investigated.
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[Expression of estrogen receptor beta in benign prostatic hyperplasia complicated by chronic prostatitis].
Zhonghua Nan Ke Xue
PUBLISHED: 05-29-2009
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To observe the expression of estrogen receptor-beta (ERbeta) in benign prostatic hyperplasia (BPH) complicated by chronic prostatitis, and to evaluate the correlation of chronic prostatitis with ERbeta expression.
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[Sorafenib reverses multidrug resistance of hepatoma cells in vitro].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 05-23-2009
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To explore the role of sorafenib in reversing multidrug resistance (MDR) in hepatoma BEL-7402/FU cells and its possible mechanisms.
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[Retrovirus-mediated SHP-1 gene expression in human breast cancer MDA-MB-231 cells].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 05-23-2009
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To construct a retrovirus-mediated expression system carrying human SHP-1 gene to transfer SHP-1 gene in human breast cancer MDA-MB-231 cells.
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Non-Markovian dynamics of a dissipative two-level system: nonzero bias and sub-Ohmic bath.
Phys Rev E Stat Nonlin Soft Matter Phys
PUBLISHED: 04-13-2009
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The quantum dynamics of the dissipative two-level system with nonzero bias and sub-Ohmic bath is studied by means of the perturbation approach based on a unitary transformation. It has been shown that for the sub-Ohmic bath it is necessary to use the non-Markovian approach, especially for the short time behavior of the coupled system and environment. The nonequilibrium correlation P(t) has been calculated to show that a finite bias may favor the short time coherence. The spectrum of the susceptibility chi"(omega) of the sub-Ohmic case may have a double peak structure in the range of omega>0 when the coupling alpha is relatively strong. Besides, the coherence-decoherence transition point alpha(c) is determined for different 0
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[Value of (18)F-FDG PET/CT in the diagnosis of laryngeal carcinoma].
Nan Fang Yi Ke Da Xue Xue Bao
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To assess the clinical value of (18)F-FDGPET/CT in the diagnosis of larynx carcinoma. Methods Forty-seven patients with larynx carcinoma or suspected larynx carcinoma underwent (18)F-FDG PET/CT examination within a week before therapy. The value of (18)F-FDG PET/CT in the diagnosis and staging of the malignancy was compared with that of unenhanced contrast CT.
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Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites.
J Zhejiang Univ Sci B
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To our knowledge, few reports on Demodex studied at the molecular level are available at present. In this study our group, for the first time, cloned, sequenced and analyzed the chitin synthase (CHS) gene fragments of Demodex folliculorum, Demodex brevis, and Demodex canis (three isolates from each species) from Xian China, by designing specific primers based on the only partial sequence of the CHS gene of D. canis from Japan, retrieved from GenBank. Results show that amplification was successful only in three D. canis isolates and one D. brevis isolate out of the nine Demodex isolates. The obtained fragments were sequenced to be 339 bp for D. canis and 338 bp for D. brevis. The CHS gene sequence similarities between the three Xian D. canis isolates and one Japanese D. canis isolate ranged from 99.7% to 100.0%, and those between four D. canis isolates and one D. brevis isolate were 99.1%-99.4%. Phylogenetic trees based on maximum parsimony (MP) and maximum likelihood (ML) methods shared the same clusters, according with the traditional classification. Two open reading frames (ORFs) were identified in each CHS gene sequenced, and their corresponding amino acid sequences were located at the catalytic domain. The relatively conserved sequences could be deduced to be a CHS class A gene, which is associated with chitin synthesis in the integument of Demodex mites.
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Downregulation of cold-inducible RNA-binding protein activates mitogen-activated protein kinases and impairs spermatogenic function in mouse testes.
Asian J. Androl.
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Cold-inducible RNA-binding protein (CIRP) is an RNA-binding protein that is expressed in normal testes and downregulated after heat stress caused by cryptorchidism, varicocele or environmental temperatures. The purpose of this study was to investigate the functions of CIRP in the testes. We employed RNAi technique to knock down the expression of CIRP in the testes, and performed haematoxylin and eosin staining to evaluate morphological changes following knockdown. Germ cell apoptosis was examined by terminal deoxynucleotidal transferase-mediated dUTP nick end labelling (TUNEL) assay, and mitogen-activated protein kinase (MAPK) signalling pathways were investigated by Western blotting to determine the possible mechanism of apoptosis. We found that using siRNA is a feasible and reliable method for knocking down gene expression in the testes. Compared to controls, the mean seminiferous tubule diameter (MSTD) and the thickness of the germ cell layers decreased following siRNA treatment, whereas the percentage of apoptotic seminiferous tubules increased. The p44/p42, p38 and SAPK/JNK MAPK pathways were activated after downregulation of CIRP. In conclusion, we discovered that downregulation of CIRP resulted in increased germ cell apoptosis, possibly via the activation of the p44/p42, p38 and SAPK/JNK MAPK pathways.
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Sequencing for complete rDNA sequences (18S, ITS1, 5.8S, ITS2, and 28S rDNA) of Demodex and phylogenetic analysis of Acari based on 18S and 28S rDNA.
Parasitol. Res.
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Due to the difficulty of DNA extraction for Demodex, few studies dealt with the identification and the phyletic evolution of Demodex at molecular level. In this study, we amplified, sequenced, and analyzed a complete (Demodex folliculorum) and an almost complete (D12 missing) (Demodex brevis) ribosomal DNA (rDNA) sequence and also analyzed the primary sequences of divergent domains in small-subunit ribosomal RNA (rRNA) of 51 species and in large-subunit rRNA of 43 species from four superfamilies in Acari (Cheyletoidea, Tetranychoidea, Analgoidea, and Ixodoidea). The results revealed that 18S rDNA sequence was relatively conserved in rDNA-coding regions and was not evolving as rapidly as 28S rDNA sequence. The evolutionary rates of transcribed spacer regions were much higher than those of the coding regions. The maximum parsimony trees of 18S and 28S rDNA appeared to be almost identical, consistent with their morphological classification. Based on the fact that the resolution capability of sequence length and the divergence of the 13 segments (D1-D6, D7a, D7b, and D8-D12) of 28S rDNA were stronger than that of the nine variable regions (V1-V9) of 18S rDNA, we were able to identify Demodex (Cheyletoidea) by the indels occurring in D2, D6, and D8.
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[Granzyme B-VEGF receptor-binding peptide fusion protein expressed in B. longum induces apoptosis of KDR-positive cells].
Nan Fang Yi Ke Da Xue Xue Bao
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To express granzyme B-vascular endothelial growth factor (VEGF) receptor-binding peptide (GrB-VRB) fusion protein in Bifidobacteria longum (B. longum) and investigate the effects of this fusion protein on the proliferation and apoptosis of cells expressing VEGF receptor II, the kinase domain receptor (KDR).
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Cold-inducible RNA-binding protein (CIRP) regulates target mRNA stabilization in the mouse testis.
FEBS Lett.
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Cold-inducible RNA-binding protein (CIRP) is an RNA-binding protein that is expressed in normal testis and down-regulated after heat stress. Recent studies suggest that CIRP contributes to male fertility problems but the mechanisms are unclear. The purpose of this study was to identify the likely mechanism of CIRP in reproduction. Based on the RNA-Binding Protein Immunoprecipitation-Microarray (Chip) Profiling (RIP-Chip) and biotin pull-down assays, we found that the mRNAs binding with CIRP in testis were mostly associated with translation regulator activity, antioxidant activity, envelope and reproduction, including important mRNAs related to male infertility. We also discovered that (Un)(n ?2) was the possible core recognition sequence, and the binding mRNAs increased their stabilization. Our results improve our understanding of the mechanism by which heat stress causes male infertility.
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MicroRNA-10b promotes migration and invasion through CADM1 in human hepatocellular carcinoma cells.
Tumour Biol.
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MicroRNA-10b (miR-10b) was recently reported to be dysregulated in some types of cancer and to play a role in invasion and metastasis. However, effects and potential mechanisms of action of miR-10b in the metastasis of hepatocellular carcinoma (HCC) have not been explored. In this study, we confirmed that miR-10b is highly expressed in metastatic HCC tissues and in metastatic HCC cell lines by qRT-PCR. Moreover, patients with higher miR-10b expression had significantly poorer overall survival, and high miR-10b expression was an independent predictor of poor prognosis. Inhibition of miR-10b reduced cell migration and invasion in MHCC97H cells, whereas over-expression of miR-10b in HepG2 cells increased cell migration and invasion. Bioinformatics and luciferase reporter assays revealed that miR-10b binds the 3-UTR of CADM1 mRNA and represses its translation. Western blot and qRT-PCR showed that CADM1 is inhibited by miR-10b over-expression. Silencing of CADM1 resulted in substantially increased cell motility and invasion similar to that observed with over-expression of miR-10b in HepG2 cells. These results suggest that miR-10b may positively regulate the invasion and metastasis of HCC through targeting CADM1.
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Targeting of mTORC2 prevents cell migration and promotes apoptosis in breast cancer.
Breast Cancer Res. Treat.
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Most of breast cancers are resistant to mammalian target of rapamycin complex 1 (mTORC1) inhibitors rapamycin and rapalogs. Recent studies indicate mTORC2 is emerging as a promising cancer therapeutic target. In this study, we compared the inhibitory effects of targeting mTORC1 with mTORC2 on a variety of breast cancer cell lines and xenograft. We demonstrated that inhibition of mTORC1/2 by mTOR kinase inhibitors PP242 and OSI-027 effectively suppress phosphorylation of Akt (S473) and breast cancer cell proliferation. Targeting of mTORC2 either by kinase inhibitors or rictor knockdown, but not inhibition of mTORC1 either by rapamycin or raptor knockdown promotes serum starvation- or cisplatin-induced apoptosis. Furthermore, targeting of mTORC2 but not mTORC1 efficiently prevent breast cancer cell migration. Most importantly, in vivo administration of PP242 but not rapamycin as single agent effectively prevents breast tumor growth and induces apoptosis in xenograft. Our data suggest that agents that inhibit mTORC2 may have advantages over selective mTORC1 inhibitors in the treatment of breast cancers. Given that mTOR kinase inhibitors are in clinical trials, this study provides a strong rationale for testing the use of mTOR kinase inhibitors or combination of mTOR kinase inhibitors and cisplatin in the clinic.
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Communication: Engineered tunable decay rate and controllable dissipative dynamics.
J Chem Phys
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We investigate the steering dissipative dynamics of a two-level system (qubit) by means of the modulation of an assisted tunneling degree of freedom which is described by a quantum-oscillator spin-boson model. Our results reveal that the decoherence rate of the qubit can be significantly suppressed and simultaneously its quality factor is enhanced. Moreover, the modulated dynamical susceptibility exhibits a multi-peak feature which is indicative of the underlying structure and measurable in experiment. Our findings demonstrate that the interplay between the combined degrees of freedom and the qubit is crucial for reducing the dissipation of qubit and expanding the coherent regime of quantum operation much large. The strategy might be used to fight against deterioration of quantum coherence in quantum information processing.
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Further evidence for the existence of major susceptibility of nasopharyngeal carcinoma in the region near HLA-A locus in Southern Chinese.
J Transl Med
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Nasopharyngeal carcinoma (NPC) is a multi-factorial malignancy closely associated with environmental factors, genetic factors and Epstein-Barr virus infection. Human leukocyte antigen (HLA) complex, specially the region near HLA-A locus, was regarded as a major candidate region bearing NPC genetic susceptibility loci in many previous studies including two recent genome-wide association (GWA) studies. To provide further evidence for the NPC susceptibility in the region near HLA-A locus based on other previous studies, we carried out a two-stage hospital-based case control association study including 535 sporadic NPC patients and 525 cancer-free control subjects from Guangdong, a high prevalence area of NPC in China.
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Complete sequence analysis of 18S rDNA based on genomic DNA extraction from individual Demodex mites (Acari: Demodicidae).
Exp. Parasitol.
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The study for the first time attempted to accomplish 18S ribosomal DNA (rDNA) complete sequence amplification and analysis for three Demodex species (Demodex folliculorum, Demodex brevis and Demodex canis) based on gDNA extraction from individual mites. The mites were treated by DNA Release Additive and Hot Start II DNA Polymerase so as to promote mite disruption and increase PCR specificity. Determination of D. folliculorum gDNA showed that the gDNA yield reached the highest at 1 mite, tending to descend with the increase of mite number. The individual mite gDNA was successfully used for 18S rDNA fragment (about 900 bp) amplification examination. The alignments of 18S rDNA complete sequences of individual mite samples and those of pooled mite samples ( ? 1000mites/sample) showed over 97% identities for each species, indicating that the gDNA extracted from a single individual mite was as satisfactory as that from pooled mites for PCR amplification. Further pairwise sequence analyses showed that average divergence, genetic distance, transition/transversion or phylogenetic tree could not effectively identify the three Demodex species, largely due to the differentiation in the D. canis isolates. It can be concluded that the individual Demodex mite gDNA can satisfy the molecular study of Demodex. 18S rDNA complete sequence is suitable for interfamily identification in Cheyletoidea, but whether it is suitable for intrafamily identification cannot be confirmed until the ascertainment of the types of Demodex mites parasitizing in dogs.
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Overexpression of Cks1 increases the radiotherapy resistance of esophageal squamous cell carcinoma.
J. Radiat. Res.
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The Cks1 protein is a member of the highly conserved family of Cks/Suc1 proteins, which interact with Cdks, and was found to be an essential cofactor for efficient Skp2-dependent ubiquitination of p27. The present study was undertaken to examine the expression status of Cks1 in esophageal squamous cell carcinoma and its significance.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.