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Find video protocols related to scientific articles indexed in Pubmed.
Three new species of Simulium (Nevermannia) (Diptera: Simuliidae) from Vietnam.
Zootaxa
PUBLISHED: 09-24-2014
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Three new species of black flies, Simulium (Nevermannia) langbiangense, S. (N.) phami, and S. (N.) bachmaense, are described on the basis of females, males, pupae and larvae collected in Vietnam. All three species are assigned to the Simulium feuerborni species-group of Simulium (Nevermannia). Simulium (N.) langbiangense sp. nov. is characterized by the female sensory vesicle with a large opening, and a short common basal stalk of the six pupal gill filaments, S. (N.) phami sp. nov. is most striking in having the pupal gill with five filaments, a character not reported in species of the S. feuerborni species-group, and S. (N.) bachmaense sp. nov. is characterized by the female genital fork with a triangular lobe-like projection pointed posteromedially on each arm, and the small larval postgenal cleft. This represents the first record of the S. feuerborni species-group from Vietnam.
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A new species and species-group of Simulium (Simulium) (Diptera: Simuliidae) from Thailand.
J. Med. Entomol.
PUBLISHED: 08-15-2014
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Simulium (Simulium) atipornae sp. nov. is described from females, males, pupae, and larvae in Thailand. This new species is characterized in the female by the claw with a small subbasal tooth, ovipositor valve triangular with its inner margin nearly straight; in the male by the style with a short subbasal protuberance and ventral plate Y-shaped, with toothed posterior margin; and in the pupa by the head and thoracic integument almost bare and gill with six filaments. Taxonomic notes are given to compare this new species with nine related species. A new species-group, the christophersi species-group, is proposed to accommodate S. atipornae sp. nov. and nine related species.
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New species and records of black flies (Diptera: Simuliidae) from Vinh Phuc Province, Vietnam.
Zootaxa
PUBLISHED: 07-17-2014
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Four new species of black flies are described, and three others are reported as newly recorded, based on adults reared from pupae, pupae and larvae collected in and near Tam Dao National Park, Vinh Phuc Province, Vietnam. New species include Simulium (Gomphostilbia) hongthaii sp. nov., S. (G.) tamdaoense sp. nov. (both species placed in the asakoae species-group), S. (Simulium) taythienense sp. nov. and S. (S.) xuandai sp. nov. (the two latter species placed in the striatum species-group). Newly recorded species are S. (G.) brinchangense Takaoka, Sofian-Azirun & Hashim, S. (Nevermannia) aureohirtum Brunetti and S. (S.) brevipar Takaoka & Davies. These discoveries increase the number of species of black flies known in Vietnam from 21 to 28. 
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A new species of Simulium (Gomphostilbia) (Diptera: Simuliidae) from Malaysia, with keys to 32 species of the Simulium ceylonicum species-group.
J. Med. Entomol.
PUBLISHED: 06-06-2014
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Simulium (Gomphostilbia) leparense sp. nov. is described from females, males, and pupae collected from Peninsular Malaysia. This new species is assigned to the ceylonicum species-group of the subgenus Gomphostilbia, and is characterized by the female and male scuta covered with dark-brown short hairs, smaller number of male upper-eye facets, presence of shiny paired spots on the male abdominal segments 2-8, and absence of grapnel-shaped hooklets on the pupal abdominal segment 9. The male and pupa of S. capillatum Takaoka, which was originally described from larvae collected from Sarawak and Sabah, are described for the first time. Keys to identify all 32 species of the Simulium ceylonicum species-group including 27 species from other countries are provided for females, males, pupae, and mature larvae.
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A two center 320 slice CT study for evaluating coronary arteries in subjects with chronic atrial fibrillation: A comparison of prospective and retrospective ECG-gating acquisition.
Int. J. Cardiol.
PUBLISHED: 05-23-2014
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To compare diagnostic-accuracy of prospective and retrospective-ECG-gated acquisition in 320-slice-CT for detecting coronary-artery stenosis in subjects with chronic-atrial-fibrillation (CAF) in a two-center study.
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CHA2DS2-VASc score is a useful-predictor of not prognosis but coronary-arteriosclerosis in chronic atrial-fibrillation compared with CHADS2 score: A two-center study of 320-slice CT, part 2.
Int. J. Cardiol.
PUBLISHED: 05-23-2014
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The aim of this study is to predict the risk of coronary-arteriosclerosis and prognosis in subjects with chronic-atrial-fibrillation (CAF) using the CHADS2 and CHA2DS2-VASc scores by 320-slice-CT and invasive-coronary-angiography (ICA) in a two-center-study.
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A new species of Simulium (Gomphostilbia) (Diptera: Simuliidae) from Thailand, with keys to 11 species of the Simulium varicorne species-group.
J. Med. Entomol.
PUBLISHED: 04-15-2014
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Simulium (Gomphostilbia) piroonae sp. nov. is described on the basis of females, males, pupae, and mature larvae collected in Mae Hong Son Province, Thailand. This new species is placed in the chumpornense subgroup of the varicorne species-group in the subgenus Gomphostilbia by having the antenna with eight flagellomeres, the pleural membrane bare, the female subcosta lacking hairs, and the pupal gill basally divided into two somewhat inflated branches. It is distinguished from all 10 known species of this group by the pupal gill with six filaments. Keys to identify 11 species of the varicorne species-group are provided for females, males, pupae, and mature larvae.
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Detection of broken sutures and metal-ring fractures in AneuRx stent-grafts by using three-dimensional CT angiography after endovascular abdominal aortic aneurysm repair: association with late endoleak development and device migration.
Radiology
PUBLISHED: 03-21-2014
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To determine the prevalence of demonstrable stent-graft degradation by using three-dimensional computed tomographic (CT) angiography to assess endoleak and stent-graft migration after endovascular aortic aneurysm repair (EVAR).
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Two new species of Simulium (Gomphostilbia) (Diptera: Simuliidae) from peninsular Malaysia, with keys to 10 peninsular Malaysian species of the Simulium batoense species group.
J. Med. Entomol.
PUBLISHED: 03-11-2014
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Two new blackfly species, Simulium (Gomphostilbia) azhari and Simulium (Gomphostilbia) johorense, are described based on adult females, males, pupae, and larvae collected from Peninsular Malaysia, and assigned to the parahiyangum subgroup and the duolongum subgroup of the batoense species group of the subgenus Comphostilbia, respectively. S. (G.) azhari sp. nov. is characterized in the female by the narrow frons, and in the male by the broad style and the ventral plate moderately produced ventrally. S. (G.) johorense sp. nov. is also remarkable in having the female subcosta lacking hairs or bearing a reduced number of hairs ranging from one to five. The pupae of both new species share a similar arrangement of the eight gill filaments (i.e., stalks of dorsal and middle triplets and ventral pair arising at the same level from the short common basal stalk), although relative lengths of filaments of the ventral pair to those of the dorsal and middle triplets are different between the two new species. Taxonomic notes are given to distinguish these new species from other related species. Keys to identify all 10 species of the batoense species group in Peninsular Malaysia are provided for adult females, males, pupae, and mature larvae.
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Two new species of the Simulium batoense species-group of Simulium (Gomphostilbia) (Diptera: Simuliidae) from Peninsular Malaysia.
Zootaxa
PUBLISHED: 03-07-2014
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Two new black fly species, Simulium (Gomphostilbia) tekamense and Simulium (Gomphostilbia) jerantutense, are described based on adult females emerged from pupae in Peninsular Malaysia, and assigned to the binuanense subgroup of the batoense species-group in the subgenus Gomphostilbia. Simulium (G.) tekamense sp. nov. is characterized in the female by the subcosta with 0-2 hairs, and presence of a  deep notch on the apex of the mediolongitudinal ridge of the cibarium, and in the pupa by one of two paired gill filaments of the middle triplet much thicker than the counter filament. Simulium (G.) jerantutense sp. nov. is characterized in the female by the short claw tooth 0.46 times the length of the claw, and in the pupa by the gill filaments arranged as [2+1+(1+2)]+2 filaments from dorsal to ventral. Taxonomic notes are given to distinguish these new species from related species.
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Mitochondrial DNA markers reveal high genetic diversity but low genetic differentiation in the black fly Simulium tani Takaoka & Davies along an elevational gradient in Malaysia.
PLoS ONE
PUBLISHED: 01-01-2014
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The population genetic structure of Simulium tani was inferred from mitochondria-encoded sequences of cytochrome c oxidase subunits I (COI) and II (COII) along an elevational gradient in Cameron Highlands, Malaysia. A statistical parsimony network of 71 individuals revealed 71 haplotypes in the COI gene and 43 haplotypes in the COII gene; the concatenated sequences of the COI and COII genes revealed 71 haplotypes. High levels of genetic diversity but low levels of genetic differentiation were observed among populations of S. tani at five elevations. The degree of genetic diversity, however, was not in accordance with an altitudinal gradient, and a Mantel test indicated that elevation did not have a limiting effect on gene flow. No ancestral haplotype of S. tani was found among the populations. Pupae with unique structural characters at the highest elevation showed a tendency to form their own haplotype cluster, as revealed by the COII gene. Tajima's D, Fu's Fs, and mismatch distribution tests revealed population expansion of S. tani in Cameron Highlands. A strong correlation was found between nucleotide diversity and the levels of dissolved oxygen in the streams where S. tani was collected.
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Simulium (Asiosimulium) furvum, a new species of black fly (Diptera: Simuliidae) from Thailand.
J. Med. Entomol.
PUBLISHED: 06-28-2013
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Simulium (Asiosimulium) furvum sp. nov. (Diptera: Simuliidae) is described from female, male, pupal, and larval specimens collected from Maewa National Park, Lampang Province, Thailand. This new species represents the fourth member of the subgenus Asiosimulium Takaoka & Chochoote, one of two small black fly subgenera endemic in the Oriental Region. It is characterized by a pear-shaped spermatheca in the female; a ventral plate in the male with a laterally compressed median keel directed ventrally and with a deep notch posteromedially, and aedeagal membrane with stout spines; and by 22 gill filaments in the pupa. Taxonomic notes are provided to separate this new species from three known species, Simulium (Asiosimulium) oblongum Takaoka & Choochote and Simulium (Asiosimulium) wanchaii Takaoka & Choochote, both from Thailand, and Simulium (Asiosimulium) suchitrae Takaoka from Nepal.
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Determination of optimum periods between onset of suspected acute myocarditis and ¹?F-fluorodeoxyglucose positron emission tomography in the diagnosis of inflammatory left ventricular myocardium.
Int. J. Cardiol.
PUBLISHED: 06-11-2013
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To determine optimum periods for (18)F-fluorodeoxyglucose (FDG) positron emission tomography (PET) examination in subjects with suspected acute myocarditis, we compared (18)F-FDG PET with endomyocardial biopsy (EMB) using the latest definition of (18)F-FDG PET for inflammatory left ventricular (LV) myocardium.
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Morphological and molecular characterization of a microsporidian parasite, Takaokaspora nipponicus n. gen., n. sp. from the invasive rock pool mosquito, Ochlerotatus japonicus japonicus.
J. Invertebr. Pathol.
PUBLISHED: 06-03-2013
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A new genus and species of Microsporidia, Takaokaspora nipponicus n. gen., n. sp. is described from Ochlerotatus japonicus japonicus (Theobald) and Ochlerotatus hatorii (Yamada) based on light microscope and ultrastructural morphology, developmental features, transmission cycles and comparative sequence analyses of the small subunit ribosomal DNA (SSU rDNA). The microsporidium is both vertically and horizontally transmitted, exhibits dimorphic development alternating between diplokaryotic and monokaryotic stages and produces two morphologically distinct spores, one in larvae and another in adult females. Horizontal transmission of infection to larval mosquitoes occurs via direct oral ingestion of uninucleate spores that are produced in vertically-infected larval hosts. Development in horizontally-infected hosts is diplokaryotic following karyokinesis of uninucleate schizonts and binary fission to produce small (4.3?m × 2.0?m) membrane free, ovoid, binucleate spores that are confined to adult female reproductive tissues (ovariole sheath and oviducts). Vertical transmission of the microsporidium from adult females to larval progeny takes place via surface contamination of the egg (transovum). Microsporidian development in vertically-infected larvae is haplophasic with unpaired nuclei throughout, producing rosette-shaped sporogonial plasmodia contained within a thin non-persistent sporophorous vesicle and culminating in the formation of membrane free, uninucleate, conical spores (7.0?m×2.8?m). Development is confined to host fat body tissue which appears as swollen white masses in the thorax and selected segments of the abdomen causing larvae to appear abnormally distorted and results in death during the third and fourth instar stages. The SSU rDNA sequences obtained from the two morphologically identical microsporidia isolated from Oc. j. japonicus and Oc. hatorii were nearly identical and unique when compared with GenBank entries of all other mosquito-parasitic species. Phylogenetic trees constructed by Maximum Parsimony, Maximum Likelihood and bootstrap analyses using the Neighbor Joining search parameter yielded similar typologies. In each case, the novel microsporidium was the sister group to the clade containing Parathelohania species from Anopheles mosquitoes and the monotypic Novothelohania ovalae from Ochlerotatus caspius showing approximately 10-13% sequence divergence to those two genera providing strong support for establishment as a separate genus.
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Anatomical relationship of coronary sinus/great cardiac vein and left circumflex coronary artery along mitral annulus in atrial fibrillation before radiofrequency catheter ablation using 320-slice CT.
Int. J. Cardiol.
PUBLISHED: 05-23-2013
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We evaluated anatomical relationships between the coronary sinus and great cardiac vein (CS/GCV) and left circumflex coronary artery (LCX) along the mitral annulus (MA) in patients with atrial fibrillation (AF) using 320-slice CT.
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Detection of adrenal veins on selective retrograde CT adrenal venography in comparison with digital subtraction angiography in subjects with established diagnosis of one-sided adrenal aldosterone-producing tumor confirmed by adrenal vein sampling, histopa
Int. J. Cardiol.
PUBLISHED: 04-06-2013
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Adrenal vein (AV) sampling (AVS) is the diagnostic gold standard for primary aldosteronism (PA), but right-sided AVS is difficult. We compared detection of AVs by selective retrograde CT adrenal venography (SRCTAV) with digital subtraction angiography (DSA).
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Diagnostic accuracy of coronary 320 slice CT angiography using retrospective electrocardiogram gated acquisition compared with virtual prospective electrocardiogram gated acquisition with and without padding.
Int. J. Cardiol.
PUBLISHED: 03-06-2013
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To reduce radiation-exposure, prospective-ECG-gating without padding is preferable. To evaluate diagnostic-accuracy of coronary 320-slice-CT angiography using various-acquisition-methods, we compared retrospective-ECG-gated with dose-modulation and "virtual" prospective-ECG-gating with and without padding.
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Specific organized substrates of ventricular fibrillation: comparison of 320-slice CT heart images in non-ischemic ventricular fibrillation subjects with non-ischemic sustained and non-sustained ventricular tachycardia subjects.
Int. J. Cardiol.
PUBLISHED: 01-23-2013
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If specific organized substrates of ventricular-fibrillation (VF) are identified, they may provide important-information for prevention of sudden-cardiac-death. To identify specific organized substrates of VF, we compared 320-slice CT heart images in non-ischemic VF subjects with non-ischemic sustained and non-sustained ventricular-tachycardia (VT) subjects.
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Infective larvae of Cercopithifilaria spp. (Nematoda: Onchocercidae) from hard ticks (Ixodidae) recovered from the Japanese serow (Bovidae).
Parasite
PUBLISHED: 01-17-2013
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Hard ticks taken from the Japanese serow, Capricornis crispus, in Yamagata Prefecture, Honshu, harboured infective larvae of onchocercid filariae after incubation from the 22nd to the 158th day. Haemaphysalis flava and H. japonica contained one to eight filarial larvae; females, males and a nymph of the ticks were infected. The 44 infective larvae recovered were 612-1,370 ?m long, and 11 of them, 930-1,340 ?m long, were studied in detail. The larvae possessed the morphologic characteristics of the larvae of the genus Cercopithifilaria, namely an oesophagus with a posterior glandular part, no buccal capsule and a long tail with three terminal lappets. Five types (A to E) of infective larvae were identified based on the morphologic characteristics. While to date five species of Cercopithifilaria have been described from the Japanese serow, a specific identification of the larvae found in this study was generally not possible. Only type E larvae could be tentatively assigned to Cercopithifilaria tumidicervicata, as they had a cervical swelling similar to that of the adults of this species. A key for the identification of the five larval types is presented. The study presents circumstantial evidences indicating that H. flava and H. japonica may transmit Cercopithifilaria spp. to Japanese serows. It also suggests the possibility that such filarial larvae will be found in hard ticks anywhere, because Cercopithifilaria is distributed worldwide, though this genus generally goes unnoticed, as its microfilariae occur in the skin, not in the blood, of host animals.
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Macrogenomic evidence for the origin of the black fly Simulium suzukii (Diptera: Simuliidae) on Okinawa Island, Japan.
PLoS ONE
PUBLISHED: 01-01-2013
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To determine the geographic origin of the black fly Simulium suzukii on Okinawa Island, Japan, macrogenomic profiles derived from its polytene chromosomes were compared with those of mainland and other insular populations of S. suzukii and of the isomorphic Simulium tani species complex. The Okinawan population is a chromosomally unique cytoform, designated D, which is essentially monomorphic and differs by about 27 fixed rearrangements from the chromosomal standard sequence for the subgenus Simulium and by two fixed differences from its nearest known relative, representing the type of S. suzukii, on the main islands of Japan. Chromosomal band sequences revealed two additional, sympatric cytoforms of S. suzukii, designated A and B, each with species status, in Korea, and a third cytoform, designated C, on Hokkaido, Japan. A new cytoform, K, of S. tani from Malaysia, representing the type of S. tani, is more closely related to cytoforms in Thailand, as are populations from Taiwan previously treated as S. suzukii but more closely aligned with S. tani and newly recognized as cytoform L of the latter nominal species. Rooting of chromosomal band sequences by outgroup comparisons allowed directionality of chromosomal rearrangements to be established, enabling phylogenetic inference of cytoforms. Of 41 macrogenomic rearrangements discovered in the five new cytoforms, four provide evidence for a stepwise origin of the Okinawan population from populations characteristic of the main islands of Japan. The macrogenomic approach applied to black flies on Okinawa Island illustrates its potential utility in defining source areas for other species of flies including those that might pose medical and veterinary risks.
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Impact of quantitatively determined native thoracic aortic tortuosity on endoleak development after thoracic endovascular aortic repair.
AJR Am J Roentgenol
PUBLISHED: 11-24-2011
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The objective of our study was to assess whether there is an association between native thoracic aortic curvature and the development of endoleaks after thoracic endovascular aortic repair.
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Simulium (Gomphostilbia) taitungense, a new species of black fly (Diptera: Simuliidae) from Taiwan, with description of the male of Simulium (Gomphostilbia) tuenense Takaoka.
Trop Biomed
PUBLISHED: 04-28-2011
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Simulium (Gomphostilbia) taitungense sp. nov. is described on the basis of reared adult, pupal and mature larval specimens collected from Taitung, Taiwan. This new species is placed in the ceylonicum species-group within the subgenus Gomphostilbia and is distinguished from related known species by the characteristic colour markings on the ventral surface of the head capsule and on the dorsal surface of the abdomen in the larva. The male of Simulium (Gomphostilbia) tuenense Takaoka, which was originally described from a pharate pupa and a larva, is described for the first time; the association of the adult stage with the larval stage was confirmed by the comparison of the sequences of the mitochondrial 16S rRNA gene; this species is also placed in the ceylonicum species-group.
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Comparison of image characteristics of plaques in culprit coronary arteries by 64 slice CT and intravascular ultrasound in acute coronary syndromes.
Int. J. Cardiol.
PUBLISHED: 03-26-2011
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To evaluate plaque image characteristics in coronary artery culprit-lesions in subjects with acute coronary syndromes (ACS), we retrospectively compared coronary arterial images by 64-slice CT before conventional-coronary-angiogram with those by intravascular ultrasound (IVUS).
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A new species of Simulium (Nevermannia) (Diptera, Simuliidae) from Thailand, with keys to members of the Simulium feuerborni species-group in Thailand.
Zookeys
PUBLISHED: 03-23-2011
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Simulium (Nevermannia) maeaiensesp. n. is described on the basis of female, male, pupal and larval specimens collected from Chiang Mai Province, Thailand. This species is assigned to the feuerborni species-group of the subgenus Simulium (Nevermannia), and is distinctive among this species-group in having the female cibarium furnished with numerous dark minute conical processes on the lower part, the female genital fork with a strongly sclerotized horizontal bar on each arm, and six long pupal gill filaments arising nearly at the same level from the common basal stalk and lying in a horizontal plane. Identification keys to seven species of the feuerborni species-group reported from Thailand are provided for females, males, pupae and mature larvae.
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Zoonotic filariasis caused by Onchocerca dewittei japonica in a resident of Hiroshima Prefecture, Honshu, Japan.
Parasitol. Int.
PUBLISHED: 05-13-2010
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A female of Onchocerca sp. was found to be the probable causative agent of a subcutaneous nodule in the left knee of a 70-year-old man in a rural area of Hiroshima Prefecture, Honshu, the main island of Japan. We compared the characteristics of the agent with the features of the four previously suspected species found in cattle and horses in various parts of the world, as well as O. lupi and O. jakutensis that were suspected or proved, respectively, in zoonotic cases in Europe. In addition, the morphologic characteristics of this parasite were compared with those of the four Onchocerca species found in wild animals in Japan. Based on such characteristics as the large triangle ridges, the considerable distance between any two adjacent ridges, and the absence of inner cuticular striae in the longitudinal sections, we found the causative agent in the present case to be identical to the female of Onchocerca dewittei japonica. All five previous cases of zoonotic onchocerciasis in Japan had been found in Oita, Kyushu, the main southern island. This human case caused by O. dewittei japonica suggests that zoonotic onchocerciasis is liable to occur in rural areas in Japan where wild boar, Simulium vectors, and humans overlap.
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Quantitative evaluation of left atrial volumes and ejection fraction by 320-slice computed-tomography in comparison with three- and two-dimensional echocardiography: a single-center retrospective-study in 22 subjects.
Int. J. Cardiol.
PUBLISHED: 03-25-2010
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To evaluate efficacy and reproducibility of 320-slice computed tomography (CT) for measuring left atrial (LA) maximum (LAVmax) and minimum volume (LAVmin) during the cardiac cycle, we compared CT with three- and two-dimensional (3D and 2D) transthoracic echocardiogram (TTE).
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Quality of coronary arterial 320-slice computed tomography images in subjects with chronic atrial fibrillation compared with normal sinus rhythm.
Int. J. Cardiol.
PUBLISHED: 02-14-2010
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To evaluate coronary arterial image quality on 320-slice CT in subjects with chronic atrial fibrillation (CAf) vs. normal sinus rhythm (NSR).
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Suitable solutions for reconstituting the ultrasound contrast agent "Levovist" used in contrast echocardiogram: in vitro and in vivo evaluation of the influence of osmotic pressure.
Int. J. Cardiol.
PUBLISHED: 10-22-2009
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To evaluate the influence of the osmotic pressure of solutions used for reconstituting the ultrasound contrast agent "Levovist" on the degree of video intensity of the enhancement and video intensity decay in contrast echocardiogram, we used 6 solutions with different osmotic pressures in both vitro and in vivo experiments.
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Left atrial compensatory function in subjects with early stage primary hypertension assessed by using left atrial volumetric emptying fraction acquired by transthoracic echocardiography.
Int. J. Cardiol.
PUBLISHED: 10-22-2009
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To evaluate left atrial (LA) function in subjects with early stage primary hypertension (HT) and without enlargement of LA, we used transthoracic echocardiogram and measured LA volumetric emptying fraction and compared the results with those in healthy volunteers.
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Left atrial wall thickness in paroxysmal atrial fibrillation by multislice-CT is initial marker of structural remodeling and predictor of transition from paroxysmal to chronic form.
Int. J. Cardiol.
PUBLISHED: 08-26-2009
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We used ECG-gated MSCT to evaluate alterations in the LA wall in patients with paroxysmal atrial fibrillation (AF) (PAF) and compared with chronic AF (CAF) and normal sinus rhythm (NSR).
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Differentiation of diagnosis and prognoses of non-coronary arterial primary myocardial diseases with left ventricular focal myocardial thinning evaluated by multislice computed tomography.
Int. J. Cardiol.
PUBLISHED: 08-24-2009
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This study sought to differentiate diagnosis and prognoses of non-coronary arterial primary myocardial diseases with focal left ventricular myocardial (LVM) thinning evaluated by multislice-CT. Based on the presence of fibro-fatty change and asynergy in LVM, we sought to understand the clinical significance of detection of non-coronary arterial focal LVM thinning.
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Comprehensive evaluation of characteristics of left ventricular myocardium in a subject with non-coronary arterial cardiac dysfunction through segment by segment analysis using various diagnostic modalities.
Int. J. Cardiol.
PUBLISHED: 05-19-2009
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We performed segment by segment analysis for comprehensive evaluation of the characteristics of the left ventricular (LV) myocardium by multislice CT (MSCT), magnetic resonance imaging (MRI), single photon emission computed tomography (SPECT) and positron emission tomography (PET) in a patient with non-coronary arterial cardiac dysfunction. If diagnosis had been performed only by transthoracic echocardiogram and conventional coronary angiography, this subject might have been diagnosed as having dilated cardiomyopathy. However, we succeeded in the detailed evaluation of characteristics of LV myocardium non-invasively. Because of the difference in spatial resolution, MSCT and MRI could only detect focal fibrosis and MSCT could only detect fatty changes in the LV myocardium with an accurate ratio of thickness of lesions in comparison with the thickness of the whole LV myocardium. Conversely, a drawback of PET and SPECT was the partial volume effect and these methods could visualize the lesions as only diffuse decrements of attenuation and could not provide detailed information. Furthermore in MSCT, LV wall motion abnormality could be visualized by showing the ribs, sternum and descending aorta and in particular MSCT could obtain much information, including extra cardiac findings.
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Cytogenetic and molecular evidence for an additional new species within the taxon Anopheles barbirostris (Diptera: Culicidae) in Thailand.
Parasitol. Res.
PUBLISHED: 05-19-2009
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ITS2 DNA sequences of 42 isoline colonies of Anopheles barbirostris species A1 and A2 were analyzed and a new genetic species, temporarily designated as species A4 (Chiang Mai), was revealed. The large sequence divergences of the ITS2 (0.116-0.615), COI (0.023-0.048), and COII (0.030-0.040) genes between A. barbirostris species A4/A1 (Chiang Mai), A4/A2 (Phetchaburi), A4/A3 (Kanchanaburi), and A4/Anopheles campestris-like Form E (Chiang Mai) provided good supporting evidence. Species A1, A2, A3, and A4 share a mitotic karyotype of Form A (X(1), X(2), Y(1)). Crossing experiments between species A4 and the other four species yielded strong reproductive isolation producing few and/or non-hatched eggs and inviable and/or abnormal development of the reproductive system of F(1) progenies. Moreover, available F(1) hybrid larvae showed asynaptic polytene chromosome arms. Hence, molecular and cytogenetic evidence strongly support the existence of A. barbirostris species A4, which is more closely related to A. campestris-like Form E than to species A1, A2, and A3. Additionally, crossing experiments among 12 and seven isolines of different cytological forms of species A1 (A, B, C, D) and A2 (A, B), respectively, yielded fertile and viable F(1) progenies. Thus, different karyotypic forms occurring in natural populations of species A1 and A2 merely represent intraspecies variation of sex chromosomes due to the extra blocks of heterochromatin.
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Comparison of three techniques for evaluation of de novo asymptomatic pulmonary arterial thrombosis following deep vein thrombosis in total knee arthroplasty.
Int. J. Cardiol.
PUBLISHED: 05-15-2009
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Deep venous thrombosis (DVT) following total knee arthroplasty (TKA) frequently results in pulmonary arterial thrombosis (PAT). Using multislice-CT (MSCT), we evaluated the incidence of de novo asymptomatic PAT following DVT in subjects undergoing TKA and compared these results by ventilation-perfusion (VP) lung scintigram and ultrasonography of the lower extremities.
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Detailed distribution of acute pulmonary thromboemboli: direct evidence for reduction of acquisition length and radiation dose for triple rule-out CT angiography.
Int. J. Cardiol.
PUBLISHED: 05-14-2009
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To reduce the redundant acquisition range and total radiation dose for planning appropriate "triple rule-out" CT angiography (CTA) for acute chest pain, we evaluated the detailed distribution of pulmonary thromboemboli (PTE) in subjects with acute PTE.
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Cardiac sarcoidosis complicated with atrioventricular block and wall thinning, edema and fibrosis in left ventricle: confirmed recovery to normal sinus rhythm and visualization of edema improvement by administration of predonisolone.
Int. J. Cardiol.
PUBLISHED: 05-14-2009
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A 65 year-old female had a node of some kind in her right leg five years ago and was diagnosed with sarcoidosis by gallium scintigraphy. Serum angiotension-converting enzyme levels had gradually increased, and three months ago she felt palpitations and dizziness when standing. On electrocardiogram, 2:1 atrioventricular (AV) block was observed. On transthoracic echocardiogram, the basal portion of the interventricular septum (IVS) revealed wall thinning with dyskinetic motion and lack of systolic thickening, and low attenuation. The basal portion of the left ventricular (LV) posterior inferior wall revealed mild wall thickening with low attenuation. Enhanced multislice-CT revealed a thickened LV posterior wall and thinned basal portion of IVS with interstitial change suggesting presence of fibrosis or edema. Late enhancement was also observed in the basal portion of the LV posterior inferior wall and basal IVS in T1 weighted magnetic resonance imaging (MRI); in addition, an area, the center of which indicated low attenuation surrounded by high attenuation, was observed in the basal portion of the LV posterior inferior wall in T2 weighted MRI. Positron emission tomography (PET) imaging using F-18 fluoro-deoxyglucose with the subject fasted for 6 h beforehand, revealed strong uptake in the basal portion of IVS and a thickened LV posterior wall, suggesting the presence of inflammation. Administration of predonisolone was started before pacemaker implantation and clinical symptoms immediately disappeared; in addition AV block recovered to normal sinus rhythm. On a repeat MRI performed four months later, the late enhancement in T1 weighted MRI and the high attenuation surrounding low attenuation in the basal portion of the LV posterior inferior wall in T2 weighted MRI both disappeared, and we confirmed that temporary edema had also disappeared.
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Positive influence of aging on the occurrence of fat replacement in the right ventricular myocardium determined by multislice-CT in subjects with atherosclerosis.
Int. J. Cardiol.
PUBLISHED: 02-15-2009
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We evaluated predictors of fat replacement (FR) in the right-ventricular-myocardium (RVM) determined by MSCT in atherosclerotics not receiving anti-arrhythmia drugs and evaluated the relationship between the presence of FR in the RVM and the occurrence of ventricular premature beats (VPB).
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New classification of aortic dissection during the cardiac cycle as pulsating type and static type evaluated by electrocardiogram-gated multislice CT.
Int. J. Cardiol.
PUBLISHED: 02-03-2009
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In some four-dimensional images acquired by electrocardiogram (ECG)-gated multislice computed tomography (MSCT) of thoracic aortic dissection (AD), true lumen (TL) gets larger (pulsating-type), whereas in others, TL and false lumen (FL) do not change (static-type) in a cardiac cycle. We have characterized these types.
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Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand.
Mem. Inst. Oswaldo Cruz
PUBLISHED: 01-30-2009
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Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.
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Buergers disease-like vasculitis associated with Kimuras disease.
Int. J. Cardiol.
PUBLISHED: 01-07-2009
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A 46-year-old man was first diagnosed as Buergers disease according to his clinical and radiological features because he had no evidence of parasitic, allergic and connective tissue disease. Soft subcutaneous nodules suspected of lymphadenopathy on the bilateral inguinal regions were recognized after admission. Positron emission tomography scan showed the increased uptake of (18)F-fluoro-2-deoxyglucose in the bilateral inguinal regions. We finally diagnosed him as Kimuras disease based on pathologic findings and laboratory data, and started steroid therapy. The uptake of (18)F-fluoro-2-deoxyglucose disappeared and his leg pain was improved after the treatment. This is the first case report presenting a patient of Kimuras disease with Buergers disease-like vasculitis who was demonstrated by positron emission tomography.
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A new species of Simulium (Simulium) (Diptera: Simuliidae) from Langkawi Island, Peninsular Malaysia.
J. Med. Entomol.
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Simulium (Simulium) kisapense sp. nov. is described on the basis of reared adult, pupal, and larval specimens collected from Langkawi Island, Peninsular Malaysia, and placed in the multi-striatum species-group in the subgenus Simulium. This new species is characterized by the bare basal section of the female radial vein, the male ventral plate with setae, the eight pupal gill filaments divergent at an angle of >90 degrees, and the shoe-shaped cocoon with small lateral window(s). Taxonomic notes are given to separate this new species from S. (S.) hirtinervis Edwards and S. (S.) malayense Takaoka and Davies, both from Peninsular Malaysia, and several other known species from Bhutan, India, Nepal, and Thailand.
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Clinical significance of fat infiltration in the moderator band and right ventricular myocardium in multislice CT, and its association with abnormal conduction seen in electrocardiogram.
Int. J. Cardiol.
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We evaluated the clinical significance of fat infiltration in the moderator-band (MB) of the right ventricle (RV) and in the RV myocardium (RVM) and its association with conduction abnormalities in the electrocardiogram.
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Acanthocheilonema delicata n. sp. (Nematoda: Filarioidea) from Japanese badgers (Meles anakuma): description, molecular identification, and Wolbachia screening.
Parasitol. Int.
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Acanthocheilonema delicata n. sp. (Filarioidea: Onchocercidae: Onchocercinae) is described based on adult filarioids and microfilariae obtained from subcutaneous connective tissues and skin, respectively, of Japanese badgers (Meles anakuma) in Wakayama Prefecture, Japan. No endemic species of the genus had been found in Japan. Recently, some filarioids (e.g., Acanthocheilonema reconditum, Dirofilaria spp., and Onchocerca spp.) have come to light as causative agents of zoonosis worldwide. The new species was readily distinguished from its congeners by morphologic characteristics such as body length, body width, esophagus length, spicule length, and the length of microfilariae. Based on the molecular data of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene, A. delicata n. sp. was included in the clade of the genus Acanthocheilonema but differed from two other congeneric species available for study, A. viteae and A. reconditum. Acanthocheilonema delicata n. sp. did not harbor Wolbachia. It is likely that the fauna of filarioids from mammals on the Japanese islands is characterized by a high level of endemicity.
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Two new species of Simulium (Gomphostilbia) (Diptera: Simuliidae) from Peninsular Malaysia.
J. Med. Entomol.
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Two new species of black flies, Simulium (Comphostilbia) terengganuense sp. nov. and Simulium (Gomphostilbia) aziruni sp. nov. (Diptera: Simuliidae), are described on the basis of reared adult, pupal, and larval specimens collected from Peninsular Malaysia. Both species are placed in the batoense species-group within the subgenus Gomphostilbia, one of two dominant subgenera of the genus Simulium in Peninsular Malaysia as well as in the Oriental Region. Strikingly, three morphological characteristics that rarely occur in the subgenus Gomphostilbia are found in these two new species: the very narrow female frons and the mushroom-like pupal terminal hooks in S. (G.) terengganuense sp. nov. and the pupal gill composed of an inflated horn-like structure and eight slender filaments in S. (G.) aziruni sp. nov.
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Notes on black flies (Diptera: Simuliidae) from North-East India: new records of five species from Arunachal Pradesh and taxonomic reviews of two species from Assam.
Trop Biomed
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In recent surveys of black flies in Arunachal Pradesh, North-East India, five species are newly recorded from Arunachal Pradesh: Simulium (Montisimulium) nemorivagum Datta, 1973, Simulium (Gomphostilbia) darjeelingense Datta, 1973, Simulium (Gomphostilbia) decuplum Takaoka & Davies, 1995, Simulium (Simulium) barnesi Takaoka & Suzuki, 1984 and Simulium (Simulium) pradyai Takaoka & Somboon, 2008, of which the latter three species also represent new records from India. Taxonomic reviews of two species of black flies reported from Assam show that Simulium (Gomphostilbia) unum Datta, 1975 is a junior synonym of Simulium (Gomphostilbia) darjeelingense, and the larva of Simulium (Gomphostilbia) sp. nr. varicorne Edwards is identifiable as Simulium (Gomphostilbia) parahiyangum Takaoka & Sigit, 1992.
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Diagnostic accuracy of 320-slice computed-tomography for detection of significant coronary artery stenosis in patients with various heart rates and heart rhythms compared with conventional coronary-angiography.
Int. J. Cardiol.
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To evaluate the diagnostic accuracy of 320-slice CT for detection of significant coronary artery stenosis in patients with various heart rates (HR) and heart rhythms, including tachycardia and chronic atrial-fibrillation (CAF) compared with conventional-coronary-angiography (CAG).
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.