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Find video protocols related to scientific articles indexed in Pubmed.
Hypermethylation of Sp1 binding site suppresses hypothalamic POMC in neonates and may contribute to metabolic disorders in adults: impact of maternal dietary CLA.
Diabetes
PUBLISHED: 12-30-2013
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Epigenetic regulation of neuropeptide genes associated with central appetite control plays an important part in the development of nutritional programming. While POMC is critical in appetite control, the molecular mechanism of methylation-related regulation of POMC remains unclear. Based on the report that the proximal Sp1 binding site in POMC promoter is crucial for the leptin-mediated activation of POMC, the methylation of this site was investigated here in both cultured cells and postnatal mice reared by the dams with dietary supplementation of CLA. The change of milk composition made the offspring undergo the increase of food intake, the suppression of POMC, the attenuation of Sp1-promoter interaction and the hypermethylation of CpG dinucleotides at -100 and -103 within Sp1 binding site of POMC promoter, which may be associated with the decrease of hypothalamic Sp1 and/or plasma S-adenosylhomocystein. In cultured cells the methylation of the -100 CpG dinucleotides of POMC promoter blocked both the formation of Sp1-promoter complex and the leptin-induced activation of POMC. In addition, a catch-up growth and adult metabolic changes like adult hyperglycemia and insulin resistance were observed in these postnatal pups, suggesting that this CLA-mediated hypermethylation may contribute, at least in part, to the metabolic disorders.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.