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Find video protocols related to scientific articles indexed in Pubmed.
A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo.
Drug Discov Ther
PUBLISHED: 09-30-2014
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Cancer is the second leading cause of death by disease in the world. Chemotherapy is one of three major therapeutic methods for cancer treatment, but cancer cells gradually evolve resistance to chemotherapeutic reagents. For centuries, traditional Chinese medicine (TCM) was used to fight against cancer. In recent years, a number of effective component mechanisms of TCM have been increasingly illuminated. As we know, chemical structures of reagents decide or affect their activities on target pathways. Thus, we classified some antitumor-related TCM components reported in the last five years into thirteen groups by their chemical structures, such as, alkaloids, diterpenoids, triterpenes, sesquiterpenes, anthraquinones, benzoquinones, flavonoids, berbamines, xanthones, saponins, steroids, polysaccharides, and glycosides. In various cancer cell lines, these constituents target dozens of signaling pathways in vitro and in vivo. Among these components, there are three sets: i) mainly apoptosis-related groups, such as, alkaloids, diterpenoids, anthraquinones, berbamines, and xanthones, target pathways like the mitochondrial pathway, NF-?B pathway, p53 pathway and so on; ii) mainly proliferation, invasion and metastasis-related groups, such as, triterpenes, sesquiterpenes, polysaccharides, and glycosides, target pathways like the mTOR pathway, ?-catenin pathway, ERK pathway and so on; iii) both apoptosis and proliferation, invasion and metastasis-related groups, such as benzoquinones, flavonoids, saponins, and steroids, target the pathways in i) and ii) synchronously. These will provide association information between TCM components and signaling pathways to promote studies on mechanisms of effective constituents, target drug development, and combinational chemotherapy. TCM could be alternative medicine for cancer treatment in the future.
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Can gamma-glutamyl transferase levels contribute to a better prognosis for patients with hepatocellular carcinoma?
Drug Discov Ther
PUBLISHED: 07-18-2014
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Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Hepatic resection has long been considered a main treatment option for HCC, but the high rate of recurrence after hepatic resection remains a problem that impacts the prognosis and survival of patients with HCC. Thus, clarifying the factors for survival and risk factors for tumor recurrence after hepatic resection is crucial. Imaging studies are currently emphasized before selecting a treatment and predicting the prognosis for patients with HCC. Recently, laboratory testing of des-gamma-carboxyprothrombin (DCP), alpha-fetoprotein (AFP), indocyanine green 15 min after administration (ICG-R15), and ?-glutamyl transpeptidase (?-GTP) has garnered attention as a way to select treatment and predict the prognosis of patients with HCC. ?-GTP in particular has critical clinical significance as an indicator of prognosis. This indicator helps to predict prognosis and it helps with the selection of further treatment, as was revealed by studies based on different subgroups of patients published in the past 5 years. The reason for the association between ?-GTP and early recurrence and poor survival is being investigated. Preoperative laboratory results (DCP, AFP, ICG-R15, and ?-GTP) may warrant attention and need to be fully evaluated before selecting a treatment and predicting prognosis in order to improve the prognosis for patients with HCC.
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Prediction of signaling pathways involved in enterovirus 71 infection by algorithm analysis based on miRNA profiles and their target genes.
Arch. Virol.
PUBLISHED: 05-25-2014
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Enterovirus 71 (EV71) causes major outbreaks of hand, foot, and mouth disease. Host factors and signaling pathways exhibit important functions in the EV71 life cycle. We conducted algorithm analysis based on miRNA profiles and their target genes to identify the miRNAs and downstream signaling pathways involved in EV71 infection. The miRNA profiles of human rhabdomyosarcoma cells treated with interferon (IFN-)-? or IFN-? were compared with those of cells infected with EV71. Genes targeted by differentially expressed miRNAs were identified and assigned to different signaling pathways according to public databases. The results showed that host miRNAs specifically responded to the viral infection and IFN treatment. Some miRNAs, including miR-124 and miR-491-3p, were regulated in opposite manners by the IFNs and EV71. Some signaling pathways regulated by both EV71 infection and IFN treatment were also predicted. These pathways included axon guidance, Wingless/Int1 (Wnt) signaling cascade, platelet-derived growth factor receptor (PDGFR)/PDGF, phosphatidylinositol 3-kinase (PI3K), Jun N-terminal kinase (JNK)/mitogen-activated protein kinase (MAPK), transforming growth factor-beta receptor (TGF-?R)/TGF-?, SMAD2/3, insulin/insulin-like growth factor (IGF), bone morphogenetic protein (BMP), CDC42, ERB1, hepatocyte growth factor receptor (c-Met), eukaryotic translation initiation factor 4E (eIF4E), protein kinase A (PKA), and IFN-? pathways. The identified miRNA and downstream signaling pathways would help to elucidate the interaction between the virus and the host. The genomics method using algorithm analysis also provided a new way to investigate the host factors and signaling pathways critical for viral replication.
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Measures to combat H7N9 virus infection in China: live poultry purchasing habits, poultry handling, and living conditions increase the risk of exposure to contaminated environments.
Biosci Trends
PUBLISHED: 09-24-2013
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From March 31 to May 31, 2013, 132 cases of humans were infected with the H7N9 avian influenza virus, 39 of which resulted in death in China, which sparked global concerns about public health. Fortunately, no new case was reported in China since May 8, which seems like to make it step into a stable stage, and the emergency response to the event launched by Shanghai, Jiangsu, Zhejiang, Anhui, Jiangxi, Shandong, and Hunan of China have been terminated currently. However, on July 20 and August 10, two new cases were reported from two provinces--Hebei and Guangdong--where no case was reported during the period of spring of 2013. The emerged two new cases rung an alarm bell, thus, the continued public health response cannot let down its guard. Based on our before studies, we found that live poultry purchasing habits, poultry handling, and living conditions increase the risk of exposure to H7N9 virus contaminated environments in China. Due to the difficulty in changing live poultry purchasing habits and in thoroughly removing or closing live poultry markets in China, we suggest that enhanced regulation of poultry markets would be a more feasible and effective strategy to fight against H7N9 virus infection in China. Moreover, in view of the fact that frequent and inevitable contact between rural residents and poultry where rural residents lived also exists due to poultry handling and living conditions, the enhanced regulations on environmental health are also needed for free-range poultry, especially in rural areas.
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Methicillin-resistant Staphylococcus aureus antibiotic resistance and virulence.
Biosci Trends
PUBLISHED: 07-10-2013
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Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most critical causes of healthcare-related or community-related infections. Resistance to most ?-lactam antibiotics makes MRSA a big threat to clinical treatment. Utilization of low efficiency antibiotics such as vancomycin and teicoplanin makes new choices for therapies. Recently, much researchhas shed light on relevance between genetic mutations of MRSA and clinical characteristics such as antibiotic resistance, and virulence. These findings could contribute to development of novel antibiotics and vaccines.
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Consensus rules in variant detection from next-generation sequencing data.
PLoS ONE
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A critical step in detecting variants from next-generation sequencing data is post hoc filtering of putative variants called or predicted by computational tools. Here, we highlight four critical parameters that could enhance the accuracy of called single nucleotide variants and insertions/deletions: quality and deepness, refinement and improvement of initial mapping, allele/strand balance, and examination of spurious genes. Use of these sequence features appropriately in variant filtering could greatly improve validation rates, thereby saving time and costs in next-generation sequencing projects.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.