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Find video protocols related to scientific articles indexed in Pubmed.
Analysis of spatial distribution characteristics of dissolved organic matter in typical greenhouse soil of northern China using three dimensional fluorescence spectra technique and parallel factor analysis model.
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 11-01-2014
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The aim of the present work is to study the soil DOM characteristics in the vegetable greenhouse with a long-term of cultivation. Results showed that the soil DOM mainly consisted of three components, fulvic acid-like (C1), humic acid-like (C2) and protein-like (C3), with C1 as the majority one. The distribution of DOM in space was also studied. In vertical direction, C1 and C2 decreased significantly with the increase in soil depth, while C3 component decreased after increased. The humification coefficient decreased fast from 0-20 to 30-40 cm, and then increased from 30-40 to 40-50 cm. In the horizontal direction, the level of C2 component varied greatly in space, while that of C1 component changed little, and that of C3 component fell in between the above two. The change in the humification degree of each soil layer significantly varied spatially. Humification process of soil organic matter mainly occurred in the surface soil layer. In addition, the humification degree in space also changed significantly. The new ideas of this study are: (1) Analyze the composition and spatial heterogeneity of soil DOM in the vegetable greenhouse; (2) Use three dimensional fluorescence spectra technology and parallel factor analysis model successfully to quantify the components of soil DOM, which provides a new method for the soil DOM analysis.
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[Evaluation of greenlight photoselective vaporization of prostate and plasmakinetic resection of prostate for the treatment of eldly benign prostatic hyperplasia men with high surgical risk: a prospective randomised control trial].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 10-08-2014
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To compare the clinical effects and safety between greenlight photoselective vaporization of prostate (PVP) and plasmakinetic resection of prostate (PRP) in eldly benign prostatic hyperplasia (BPH) men with high surgical risk. METHODS; Fifty three eldly BPH patients, who had the indication of surgical treatment but complicated with at least one internal disease, were randomly divided into PVP group and PRP group. The clinical data of all the patients were collected and compared between the two groups, including pre-operative and post-operative international prostate symptom score (IPSS), postvoid residual urine (PVR), urine flow rate (Qmax), quality of life (QOL) as well as prostate volume (PV), operational time, operative bleeding volume, postoperative recovery, peri-operative complications.
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Association mapping for partial resistance to Phytophthora sojae in soybean (Glycine max (L.) Merr.).
J. Genet.
PUBLISHED: 09-06-2014
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Association mapping is a powerful high-resolution mapping tool for complex traits. The objective of this study was to identify QTLs for partial resistance to Phytophthora sojae. In this study, we evaluated a total of 214 soybean accessions by the hypocotyl inoculation method, and 175 were susceptible. The 175 susceptible accessions were then evaluated for P. sojae partial resistance using slant board assays. The 175 accessions were screened with 138 SSR markers that generated 730 SSR alleles. A subset of 495 SSR loci with minor allele frequency (MAF) ? 0.05 was used for association mapping by the Tassel general linear model (GLM) and mixed linear model (MLM) programmes. This soybean population could be divided into two subpopulations and no or weak relatedness was detected between pairwise accessions. Four SSR alleles, Satt634-133, Satt634-149, Sat_222-168 and Satt301-190, associated with partial resistance to P. sojae were detected by both GLM and MLM methods. Of these identified markers, one marker, Satt301, was located in regions where P. sojae resistance QTL have been previously mapped using linkage analysis. The identified markers will help to understand the genetic basis of partial resistance, and facilitate future marker-assistant selection aimed to improve resistance to P. sojae and reduce disease-related mortality in soybean.
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3.0T MRI arterial spin labeling and magnetic resonance spectroscopy technology in the application of Alzheimer's disease.
Exp. Gerontol.
PUBLISHED: 07-31-2014
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The purpose of this study was to investigate the changes in the cerebral blood flow (CBF) and spectrum variables in the posterior cingulate region of patients with AD under the detection of arterial spin labeling (ASL) and magnetic resonance spectroscopy (MRS). A total of 20 AD patients (8 males and 12 females; mean age, 64.84±8.82years) and 20 healthy controls (9 males and 11 females; mean age, 64.94±7.93years) were included in this study for analysis. All images were obtained using a 3.0-T MR imager and an 8-channel head array receiving coil. MRS measurements were conducted exploring variables of metabolite ratios. Statistical analyses were conducted with the SPSS 11.0 statistical software package. Findings in the present study revealed a significant difference in the mean MMSE scores between the AD group and the healthy control group (16.21±4.01 vs. 27.35±1.01, P<0.01). Compared with the healthy control group, CBF in the bilateral frontal region showed a significant decrease in the AD group (right frontal: 83.5±7.2 vs. 110±11.5, P<0.05; left frontal: 85.6±8.1 vs. 108.7±12.2, P<0.05, respectively), and a similar association was also observed in the TL, TPJ, parietal, and hippocampal regions (all P<0.05). MRS imaging in the posterior cingulate region showed a significant reduction in the NAA/Cr ratio in the AD group (1.43±0.1 vs. 1.49±0.0, P<0.05). Additionally, we found that the MI/Cr and Cho/Cr ratios were higher than normal controls in patients with AD (all P<0.05). Our results suggested that 3D ASL detection combined with MRS in studying AD could show the regional hypo-perfusion with the decrease of CBF and the abnormal metabolic changes of the posterior cingulate cortex.
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Ubiquitin-like protein FAT10 promotes the invasion and metastasis of hepatocellular carcinoma by modifying ?-catenin degradation.
Cancer Res.
PUBLISHED: 07-23-2014
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The ubiquitin-like protein FAT10 and the homeobox protein HOXB9 each promote metastatic progression in hepatocellular carcinoma (HCC). In this study, we investigated the clinicopathologic significance of FAT10 and HOXB9 in HCC and investigated a mechanistic role for FAT10 in HOXB9-mediated invasiveness and metastasis. Relative to adjacent normal tissues, FAT10 and HOXB9 were markedly overexpressed in HCC, where a positive correlation in their expression and associated malignant characteristics were found. RNAi-mediated silencing of FAT10 decreased HOXB9 expression and inhibited HCC invasion and metastasis in vitro and in vivo. The effects of FAT10 silencing were reversed by HOXB9 overexpression, whereas RNAi-mediated silencing of HOXB9 decreased HCC invasion and metastasis driven by FAT10 overexpression. Mechanistically, FAT10 regulated HOXB9 expression by modulating the ?-catenin/TCF4 pathway, directly binding to ?-catenin and preventing its ubiquitination and degradation. Together, our results identified a novel HCC regulatory circuit involving FAT10, ?-catenin/TCF4, and HOXB9, the dysfunction of which drives invasive and metastatic character in HCC. Cancer Res; 74(18); 5287-300. ©2014 AACR.
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[Establishment and application of Chinese Pediatric Evaluation of Disability Inventory norms in Chongqing, China].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 06-14-2014
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To establish the Chinese Pediatric Evaluation of Disability Inventory (PEDI) norms in Chongqing, China.
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[Circumcision with no-flip Shang Ring technique for adult males: analysis of 168 cases].
Zhonghua Nan Ke Xue
PUBLISHED: 05-31-2014
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To observe the clinical effects of the no-flip procedure with the Chinese Shang Ring when circumcising adult males with redundant prepuce or phimosis, and to discuss its advantages and disadvantages.
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Sex disparity and perception of obesity/overweight by parents and grandparents.
Paediatr Child Health
PUBLISHED: 05-06-2014
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To explore the factors associated with the sex disparity showing a greater prevalence of obesity/overweight in boys compared with girls in Chinese school children.
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Soya and isoflavone intakes associated with reduced risk of oesophageal cancer in north-west China.
Public Health Nutr
PUBLISHED: 03-29-2014
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To ascertain the association between soya consumption, isoflavone intakes and oesophageal cancer risk in remote north-west China, where the incidence of oesophageal cancer is known to be high.
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Electromagnetic interference shielding of segregated polymer composite with an ultralow loading of in situ thermally reduced graphene oxide.
Nanotechnology
PUBLISHED: 03-14-2014
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An in situ thermally reduced graphene/polyethylene conductive composite with a segregated structure was fabricated, which achieved a high electromagnetic interference shielding effectiveness of up to 28.3-32.4 dB at an ultralow graphene loading of 0.660 vol.%. Our work suggests a new way of effectively using graphene.
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No association between XRCC1 gene Arg194Trp polymorphism and risk of lung cancer: evidence based on an updated cumulative meta-analysis.
Tumour Biol.
PUBLISHED: 02-10-2014
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X-ray repair cross-complementing group 1 (XRCC1) gene Arg194Trp polymorphism has been reported to be associated with risk of lung cancer in many published studies. Nevertheless, the research results were inconclusive and conflicting. To reach conclusive results, several meta-analysis studies were conducted by combining results from literature reports through pooling analysis. However, these previous meta-analysis studies were still not consistent. Hence, we used an updated and cumulative meta-analysis to get a more comprehensive and precise result from 25 case-control studies searching through the PubMed database up to September 1, 2013. The meta-analysis was carried out by the Comprehensive Meta-Analysis software and the odds ratio (OR) with 95 % confidence interval (CI) was used to estimate the pooled effect. The result involving 8,876 lung cancer patients and 11,210 controls revealed that XRCC1 Arg194Trp polymorphism was not associated with lung cancer risk [(OR=0.97, 95 %CI=0.92-1.03) for Trp vs. Arg; (OR=0.92, 95 % CI=0.85-0.98) for ArgTrp vs. ArgArg; (OR=1.07, 95 % CI=0.92-1.23) for TrpTrp vs. ArgArg; (OR=0.93, 95 % CI=0.87-1.00) for (TrpTrp?+?ArgTrp) vs. ArgArg; and (OR=1.08, 95 % CI=0.94-1.25) for TrpTrp vs. (ArgTrp?+?ArgArg)]. The cumulative meta-analysis showed that the results maintained the same, while the ORs with 95 % CI were more stable with the accumulation of case-control studies. The sensitivity and subgroups analyses showed that the results were robust and not affected by any single study with no publication bias. Relevant studies might not be needed for supporting these results.
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Interleukin-1 receptor blockade in perinatal brain injury.
Front Pediatr
PUBLISHED: 01-01-2014
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Interleukin-1 (IL-1) is a potent inflammatory cytokine that can be produced by a variety of cell types throughout the body. While IL-1 is a central mediator of inflammation and response to infection, the role of IL-1 signaling in adult and pediatric brain injury is becoming increasingly clear. Although the mechanisms of IL-1 expression are largely understood, the downstream effects and contributions to excitotoxicity and oxidative stress are poorly defined. Here, we present a review of mechanisms of IL-1 signaling with a focus on the role of IL-1 in perinatal brain injury. We highlight research models of perinatal brain injury and the use of interleukin-1 receptor antagonist (IL-1RA) as an agent of therapeutic potential in preventing perinatal brain injury due to exposure to inflammation.
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Maternal pravastatin prevents altered fetal brain development in a preeclamptic CD-1 mouse model.
PLoS ONE
PUBLISHED: 01-01-2014
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Using an animal model, we have previously shown that preeclampsia results in long-term adverse neuromotor outcomes in the offspring, and this phenotype was prevented by antenatal treatment with pravastatin. This study aims to localize the altered neuromotor programming in this animal model and to evaluate the role of pravastatin in its prevention.
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[Treatment of knee osteoarthritis with acupuncture and moxibustion: a randomized controlled trial].
Zhongguo Zhen Jiu
PUBLISHED: 12-31-2013
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To compare the difference of the efficacy on knee osteoarthritis (KOA) between the combined therapy of acupuncture and moxibustion and western medication, and explore the better therapeutic method for KOA.
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High temperature of food and beverage intake increases the risk of oesophageal cancer in Xinjiang, China.
Asian Pac. J. Cancer Prev.
PUBLISHED: 11-02-2013
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The north-western region of China has a high incidence of oesophageal cancer. This study aimed to investigate whether the intake of food and beverage at high temperature is associated with the risk of oesophageal cancer among adults residing in this remote part of China.
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Profile of hepatitis B virus resistance mutations against nucleoside/nucleotide analogue treatment in Chinese patients with chronic hepatitis B.
Virol. J.
PUBLISHED: 09-11-2013
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Aim: Antiviral drug-resistant HBV mutants are complex and currently partly understood. This study was performed to analyze the profile of hepatitis B virus (HBV) resistance mutations against nucleos(t)ide analogues (NAs) in patients with chronic hepatitis B (CHB).
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Self-assembly and sorting of acentrosomal microtubules by TACC3 facilitate kinetochore capture during the mitotic spindle assembly.
Proc. Natl. Acad. Sci. U.S.A.
PUBLISHED: 09-03-2013
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Kinetochore capture by dynamic kinetochore microtubule fibers (K fibers) is essential for proper chromosome alignment and accurate distribution of the replicated genome during cell division. Although this capture process has been extensively studied, the mechanisms underlying the initiation of this process and the proper formation of the K fibers remain largely unknown. Here we show that transforming acidic coiled-coil-containing protein 3 (TACC3) is essential for kinetochore capture and proper K-fiber formation in HeLa cells. To observe the assembly of acentrosomal microtubules more clearly, the cells were released from higher concentrations of nocodazole into zero or lower concentrations. We find that small acentrosomal TACC3-microtubule aster formation near the kinetochores and binding of the asters with the kinetochores are the initial steps of the kinetochore capture by the acentrosomal microtubules, and that the sorting of kinetochore-captured acentrosomal microtubules with centrosomal microtubules leads to the capture of kinetochore by centrosomal microtubules from both spindle poles. We demonstrate that the sorting of the TACC3-associated microtubules with the centrosomal microtubules is a crucial process for spindle assembly and chromosome movement. These findings, which are also supported in the unperturbed mitosis without nocodazole, reveal a critical TACC3-dependent acentrosomal microtubule nucleation and sorting process to regulate kinetochore-microtubule connections and provide deep insight into the mechanisms of mitotic spindle assembly and chromosome alignment.
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Dietary fibre intake associated with reduced risk of oesophageal cancer in Xinjiang, China.
Cancer Epidemiol
PUBLISHED: 06-27-2013
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Objective: To ascertain the relationship between dietary fibre intake and risk of oesophageal cancer in remote northwest China, where the cancer incidence is known to be high. Methods: A case-control study was conducted during 2008-2009 in the Urumqi and Shihezi, Xinjiang Uyghur Autonomous Region of China. Participants were 359 incident oesophageal cancer patients and 380 hospital-based controls. Information on habitual foods consumption was obtained by face-to-face interview, from which dietary fibre intakes were estimated using the Chinese food composition tables. Results: The oesophageal cancer patients reported lower intake levels of total dietary fibre and fibre derived from vegetables and fruits than those of controls. Overall, regular intake of fibre was inversely associated with the oesophageal cancer risk, the adjusted odds ratio being 0.47 (95% confidence interval 0.32-0.69) for the highest (>27g) versus the lowest (<16g) tertile of daily intake, with a significant dose-response relationship (p=0.004). Similar reductions in risk were also apparent for high intake levels of vegetable fibre and fruit fibre, but to a lesser extent for cereal fibre. Conclusion: Habitual intake of dietary fibre was associated with a reduced risk of oesophageal cancer for adults in northwest China.
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[Analysis of correlative factors of non-surgical vertebral fractures after percutaneous vertebroplasty for osteoporotic vertebral compression fractures].
Zhongguo Gu Shang
PUBLISHED: 06-26-2013
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To investigate the correlative factors of non-surgical vertebral fractures after percutaneous vertebroplasty (PVP) for osteoporotic vertebral compression fractures(OVCFs).
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ROCK is Involved in Vimentin Phosphorylation and Rearrangement Induced by Dengue Virus.
Cell Biochem. Biophys.
PUBLISHED: 06-06-2013
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Our previous study showed that dengue virus 2 (DENV2) infection induces rearrangement of vimentin into dense structures at the perinuclear area. However, the underlying mechanism of this phenomenon is poorly characterized. In the present work, we found that vimentin and Ser71 phosphorylated vimentin display similar distributions in DENV2-infected cells. DENV2 infection also induced ROCK activation and phosphorylation of vimentin at Ser71 as the DENV2 infection progressed. Furthermore, Ser71 phosphorylation and vimentin rearrangement induced by DENV2 infection were blocked by the ROCK inhibitor Y-27632. In addition, DENV2 led to endoplasmic reticulum (ER) redistribution in the perinuclear region of the host cells, which was partially blocked by pretreatment with Y-27632. Together, these data support indicate that ROCK may have a role in governing regulating vimentin and ER rearrangement during DENV2 infection. We hypothesize that DENV2 infection, via ROCK activation, induces both vimentin rearrangement and ER redistribution around the perinuclear region, which may play a structural role in anchoring DENV2 to replication sites.
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Biomechanical Assessment of Unilateral Pedicle Screws plus Contralateral Transfacetopedicular Screws after Transforaminal Lumbar Interbody Fusion with Two Cages.
Orthop Surg
PUBLISHED: 04-30-2013
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To assess the biomechanical stability of unilateral pedicle screws (UPS) plus contralateral transfacetopedicular screws (TFPS) after transforaminal lumbar interbody fusion (TLIF) with two cages.
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The roles of autophagy and apoptosis in burn wound progression in rats.
Burns
PUBLISHED: 01-28-2013
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Understanding the role of cell death in burn wound progression is crucial for giving appropriate diagnoses and designing therapy regimens for burn patients. A well-described and reliable "comb burns model" was employed to evaluate the roles of autophagy and apoptosis in burn wound progression at 2h, 6h, 12h, 24h, and 48h post-burn in a rat model. Immunohistochemistry (IHC) results showed that autophagy was detectable in hair follicle epithelium at 2h post-burn, peaked at 12h post-burn, then declined. Conversely, apoptosis was mainly located in the stratum epidermis and took place at low levels until 6h post-burn, at which point it slowly increased. Bcl-2 and Bax, which are regulators of both processes, showed protein expression level patterns that were consistent with the IHC results. This study of autophagy in burn wound tissue progression represents a conceptual expansion of cell death in burn wounds. Based on these results, we suggest that different treatments should be performed on a specific post-burn time course depending on the most prevalent type of cell death occurring at that time.
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[Flavonoid glycosides from Androsace umbellata].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 11-30-2011
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To study the chemical constituents of Androsace umbellata.
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[Diffusion tensor imaging findings in first-episode and chronic schizophrenics].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 11-19-2011
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To investigate the integrity of white matters in first-episode and chronic schizophrenics.
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[Lamivudine-resistant analysis and management for chronic hepatitis B patients with initial lamivudine therapy].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 11-05-2011
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To study the factors influencing the long-term usage of lamivudine (LAM) in chronic hepatitis B (CHB) patients and the management after drug-resistance.
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[Recovery of reproductive endocrine function after orthotopic fetal ovarian allotransplantation in rats].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 10-27-2011
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To assess the recovery of the reproductive endocrine function in rats following orthotopic transplantation of fetal ovarian allograft.
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Targeted deletion of Hand2 in enteric neural precursor cells affects its functions in neurogenesis, neurotransmitter specification and gangliogenesis, causing functional aganglionosis.
Development
PUBLISHED: 10-13-2011
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Targeted deletion of the bHLH DNA-binding protein Hand2 in the neural crest, impacts development of the enteric nervous system (ENS), possibly by regulating the transition from neural precursor cell to neuron. We tested this hypothesis by targeting Hand2 deletion in nestin-expressing neural precursor (NEP) cells. The mutant mice showed abnormal ENS development, resulting in lethal neurogenic pseudo-obstruction. Neurogenesis of neurons derived from NEP cells identified a second nestin non-expressing neural precursor (NNEP) cell in the ENS. There was substantial compensation for the loss of neurons derived from the NEP pool by the NNEP pool but this was insufficient to abrogate the negative impact of Hand2 deletion. Hand2-mediated regulation of proliferation affected both neural precursor and neuron numbers. Differentiation of glial cells derived from the NEP cells was significantly decreased with no compensation from the NNEP pool of cells. Our data indicate differential developmental potential of NEPs and NNEPs; NNEPs preferentially differentiate as neurons, whereas NEPs give rise to both neurons and glial cells. Deletion of Hand2 also resulted in complete loss of NOS and VIP and a significant decrease in expression of choline acetyltransferase and calretinin, demonstrating a role for Hand2 in neurotransmitter specification and/or expression. Loss of Hand2 resulted in a marked disruption of the developing neural network, exemplified by lack of a myenteric plexus and extensive overgrowth of fibers. Thus, Hand2 is essential for neurogenesis, neurotransmitter specification and neural network patterning in the developing ENS.
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[Mutations in various functional domains of HPV2 E2 protein inhibit the transcriptional depression activities].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
PUBLISHED: 10-08-2011
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To study the potential transcriptional depression activities of HPV2 E2 proteins with mutations in different functional domains.
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Talatisamine, a C(19)-diterpenoid alkaloid from Chinese traditional herbal Chuanwu.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 09-28-2011
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THE TITLE COMPOUND [SYSTEMATIC NAME: (1S,4S,5R,7S,8S,9R,10R,11S,13S,14S,16S,17R)-N-methyl-8,14-dihy-droxy-1,16-tri-meth-oxy-4-(meth-oxy-methyl-ene)aconitane], C(24)H(39)NO(5), was isolated from the roots of Aconitum carmichaelii Debx., which is known as Chuanwu in Chinese traditional herbal medicine. The mol-ecule has an aconitane carbon skeleton with four six-membered rings and two five-membered rings, including a six-membered N-containing heterocyclic ring. Both five-membered rings adopt envelope conformations. The four six-membered adopt chair conformations. Two intra-molecular O-H?O hydrogen bonds occur.
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[Comparative study on repairing rabbit radius segmental defects with two different proportions of chitosan combined with allogeneic morselized bone].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
PUBLISHED: 08-09-2011
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To give a preliminary experimental evidence and to prove chitosan and allogeneic morselized bone as potential bone substitutions in repairing rabbit radius segmental defect.
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[Comparison of white matter integrity of schizophrenic patients with and without impulsive behaviors by diffusion tensor magnetic resonance imaging].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 05-23-2011
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To compare the regional white matter integrity of schizophrenics with impulsive behaviors versus those without.
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Human papillomavirus 16 variants from Zambian women with normal pap smears.
J. Med. Virol.
PUBLISHED: 05-14-2011
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Human papillomavirus (HPV) type 16 is the most prevalent high-risk viral genotype associated with cervical cancer. Six distinct phylogenetic clusters of HPVs have been identified and are distributed differently across five continents. HPV16 DNA was extracted from cervicolavage samples from women with normal pap smears. The LCR regions were amplified in triplicate, cloned, sequenced, and analyzed from a total of 11 recovered HPV16 positive samples [Ngandwe et al. (2007): BMC Infect Dis 7:77] were analyzed for sequence variation. The HPV16 LCR variants were assessed for promoter activity by use of a luciferase reporter gene. Six novel HPV16 variants with nucleotide exchanges in the LCR region were identified. Five clones were classified as European group HPV16 variants and one as an African group variant. Two of these variants had relatively lower promoter activity, 30% of that of the wild-type strain. The decreased promoter activity of some HPV16 variants may decrease expression of viral oncogenes and may be linked with the development, phenotype and severity of the cervical lesions in women infected with these across HPV16 variants.
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Characterization of a new male sterility-related gene Camf1 in Capsicum annum L.
Mol. Biol. Rep.
PUBLISHED: 04-29-2011
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For the sake of screening novel genes related to the male sterility in chili pepper for studying the molecular mechanism of plant male sterility, the gene differential expression analysis was performed by cDNA-amplified fragment length polymorphism (cDNA-AFLP) in the genic male sterile-fertile line 114AB of Capsicum annum L., and a variety of differentially expressed cDNA fragments were detected in fertile or sterility material. Camf1, a transcript-derived fragment (TDF) accumulated in fertile line flower buds was further investigated. The Camf1 has 1,854 bp in length with no introns containing a 1,707-bp open reading frame (ORF). The deduced amino acid sequence of Camf1 shares higher similarity to some members from a glyoxal oxidase-related protein family, and has a glyoxal oxidase conserved domain at the N-terminus and a domain of unknown function (DUF1929) at C-terminal end. Expression analysis showed that Camf1 expressed only in stage 3-7 flower buds of male fertile of C. annum L. 114AB, and no detection in all organs of male sterility. The peak of expression level of Camf1 appeared at stage 4 flower buds of fertile line. Furthermore, expression analysis of different organs revealed that Camf1 expressed only in anthers of male fertile material and there were no expression in sepals, petals, pistils, roots, stems, leaves and open flowers. These results suggested that Camf1 was an anther-specific gene and might be essential for the fertility of C. annum L.
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[The replication capacity and drug sensitivity of Adefovir dipivoxil-resistant HBV mutants in vivo].
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
PUBLISHED: 04-13-2011
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This study was aimed to research the changes of the biological characteristics of Hepatitis B virus-resistant mutant model in vivo. The anti-ADV mutant HBV plasmid of rtA181V and rtN236T, prepared by Multi Site-Directed Mutagenesis Kit, was transferred into mice via the tail vein, and the levels of HBV-DNA replication were detected after Anti-HBV drugs treatment. The HBV-DNA replication had been detected in the mutant mice, which means that the establishment of HBV-resistance mutant model in vivo was successful, but the level of HBV DNA replication intermediates in the anti-ADV mutant mice liver were decreased compared with wild 4.1kb HBV plasmid mice. The wild-type mice were sensitive to Lamivudine, Adefovir dipivoxil and Entecavir. Though the mutant mice were also sensitive to Entecavir, the sensitivity to lamivudine and Adefovir dipivoxil decreased. As a result, this study established the Adefovir-resistant hepatitis B virus mouse model successfully. However, the replication level of HBV-DNA was reduced in this model, and Adefovir dipivoxil in the mutant mice had no significant inhibition effect on HBV-DNA.
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[Correlation among coping style, social support, and negative emotion in infertile women].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 03-04-2011
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To explore the correlation among coping style, social support, and negative emotion in Chinese infertile women.
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Mimecan is involved in aortic hypertrophy induced by sinoaortic denervation in rats.
Mol. Cell. Biochem.
PUBLISHED: 02-24-2011
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This study was designed to investigate whether mimecan was involved in aortic hypertrophy induced by sinoaortic denervation in rats. 8 weeks after sinoaortic denervation, when compared to sham-operated rats, sinoaortic denervated rats exhibited aortic hypertrophy and down-regulation of mimecan. Through classic univariate correlation analysis, it was found that mimecan mRNA was negatively related to extent of aortic hypertrophy. Treatment of primary cultured vascular smooth muscle cells with the Ang II (1 ?M), which was found locally increased in the aortae of sinoaortic denervated rats, resulted in a reduction of mimecan expression. In vitro, knockdown of mimecan in vascular smooth muscle cells promoted cell proliferation induced by 15% of fetal bovine serum or Ang II (1 ?M). We concluded that down-regulation of mimecan was involved in aortic hypertrophy induced by sinoaortic denervation in rats.
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Cesarean delivery for first pregnancy and neonatal morbidity and mortality in second pregnancy.
Eur. J. Obstet. Gynecol. Reprod. Biol.
PUBLISHED: 02-07-2011
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To examine impact of cesarean delivery in first pregnancy on neonatal mortality and morbidity in second pregnancy.
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[Association between the influential factors and the effectiveness of pegylated interferon alpha-2a plus ribavirin as a combination treatment for chronic hepatitis C patients].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 01-29-2011
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To evaluate the efficacy and to investigate the association between the length of the treatment period and the cumulative dose of pegylated interferon alpha-2a (PegIFN alpha-2a) plus ribavirin (RBV) and the effectiveness of antiviral therapy. We analyzed data from 117 patients treated for 48 weeks with PEG-IFN alpha-2a (135mug or 180mug/week) plus weight-based RBV (800 mg/d for patients is less than or equal to 65 kg, 1000 mg/d for patients 65-75 kg and 1200 mg/d for patients is more than or equal to 75 kg) under care at West China Hospital. HCV RNA was assessed at baseline, Week 4, 12 and 24, the end of treatment (EOT) and after 24 weeks follow-up (sustained virological response; SVR) with a test range of 1.0*10(3) to 5.0*10(7) IU/ml. Patients were stratified by age, gender, weight, route of transmission, duration of infection, baseline HCV RNA level and PegIFN alpha-2a or RBV dosage. HCV genotype was assessed in 29 patients (genotype 1b, 21; genotype 2a, 7; genotype 1b/2a, 1). Rapid virological response (RVR; HCV RNA negative at week 4), complete early virological response (cEVR; HCV RNA negative at week 12), EOT response, and SVR were achieved in 88 (75.2%), 110 (94%), 114 (97.4%) and 96 (82.1%) patients, respectively. Younger age, lower weight and shorter speculated infection years were associated with higher SVR rates (91.4% vs 72.9%, x2=6.796, P value is less than 0.05; 85% vs 50%, x2=5.433, P value is less than 0.05; 96.7% vs 77%, x2=5.852, P value is less than 0.05). SVR significantly increased with treatment length (38.5%, 66.7%, and 88.8% for is less than or equal to 29 weeks, 29-38 weeks, and is more than or equal to 38 weeks, respectively). SVR significantly increased with total cumulative treatment doses (38.5%, 66.7% and 88.8% for is less than or equal to 60%, 60%-80% and is more than or equal to 80% of PegIFN dose respectively; 33.3%, 85.3% and 96.8% for is less than or equal to 60%, 60%-80% and is more than or equal to 80% in RBV dose respectively) in all patients. Less than 80% of standard dose of RBV was not sufficient even if given enough PegIFN (is more than or equal to 80% cumulative treatment dose) in patients who achieved RVR. Chinese patients treated with peginterferon alpha-2a plus ribavirin have high rates of SVR. It is important to complete the target length of treatment and to continue the target dosage to achieve SVR.
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[Observation of and research on intravital microcirculation use of orthogonal polarization multi-spectral technique].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 09-11-2010
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A small, simple intravital microcirculation multi-spectral imaging system was constructed. Orthogonal polarization spectral (OPS) imaging and liquid crystal tunable filter (LCTF) were used for intravital microcirculation multi-spectral imaging. LCTF has excellent imaging quality and low-power loss, without moving parts and image shift. It can provide continuously, rapid and random-access selection of any wavelength in the visible to near-infrared range. An ear of nude mouse was adopted as experiment specimen. The multi-spectral images of ear microvessel of the nude mouse were acquired by this imaging system in two ways (detection polarization angle 90 degrees and 0 degrees). Compared with detection polarization angle 0 degrees way the images acquired by orthogonal polarization spectral imaging way(detection polarization angle 90 degrees) had better contrast and more details. It is concluded that the experiment has proved that the orthogonal polarization multi-spectral technique can enhance the effect of observation of intravital microcirculation.
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Ligand-independent toll-like receptor signals generated by ectopic overexpression of MyD88 generate local and systemic antitumor immunity.
Cancer Res.
PUBLISHED: 09-07-2010
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Although critical for initiating and regulating immune responses, the therapeutic use of individual cytokines as anticancer immunotherapeutic agents has achieved only modest clinical success. Consequently, many current strategies have focused on the use of specific immunotherapeutic agonists that engage individual receptors of innate immune networks, such as the Toll-like receptor (TLR) system, each resulting in specific patterns of gene expression, cytokine production, and inflammatory outcome. However, these immunotherapeutics are constrained by variable cellular TLR expression and responsiveness to particular TLR agonists, as well as the specific cellular context of different tumors. We hypothesized that overexpression of MyD88, a pivotal regulator of multiple TLR signaling pathways, could circumvent these constraints and mimic coordinated TLR signaling across all cell types in a ligand-independent fashion. To explore this hypothesis, we generated an adenoviral vector expressing MyD88 and show that Ad-MyD88 infection elicits extensive Th1-specific transcriptional and secreted cytokine signatures in all murine and human cell types tested in vitro and in vivo. Importantly, in vivo intratumoral injection of Ad-MyD88 into established tumor masses enhanced adaptive immune responses and inhibited local tumor immunosuppression, resulting in significantly inhibited local and systemic growth of multiple tumor types. Finally, Ad-MyD88 infection of primary human dendritic cells, tumor-associated fibroblasts, and colorectal carcinoma cells elicited significant Th1-type cytokine responses, resulting in enhanced tumor cell lysis and expansion of human tumor antigen-specific T cells. Thus, Ad-MyD88 initiated robust antitumor activity in established murine tumor microenvironments and in human contexts, suggesting its potential effectiveness as a clinical immunotherapeutic strategy.
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Administration of anti-interleukin-6 monoclonal antibody prolongs cardiac allograft survival.
Transpl. Int.
PUBLISHED: 07-22-2010
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To investigate the effects of anti-IL-6 monoclonal antibody (anti-IL-6 mAb) on acute allograft rejection and the potential mechanisms in a mouse heart transplantation model. Heterotopical heart graft model was performed. The anti-IL-6 mAb was administered to recipient mice after cardiac grafting. Results were compared with administration of anti-IL-17 mAb or anti-IL-6 mAb+anti-IL-17 mAb (the double treatment). The cardiac allograft survival was monitored by daily palpation in combination with histological evaluation. Quantitative polymerase chain reaction assay, mixed lymphocyte reaction, and flow cytometric analysis were employed to determine the mRNA expression of pro-inflammatory cytokines, allogeneic T-cell proliferation, and the proportion of CD4(+) CD25(+) Foxp3(+) regulatory T cells in graft-infiltrating lymphocytes and splenocytes of recipients, respectively. The results showed that the cardiac allograft survival in anti-IL-6 mAb-treated mice was prolonged significantly when compared with that of the untreated or anti-IL-17 mAb-treated mice. Meanwhile, the double-treated did not prolong graft survival significantly when compared with those treated with anti-IL-6 mAb. The increase of graft survival induced by anti-IL-6 mAb was associated with reduced transcript levels for IFN-? and IL-17, accompanied by a dramatic reduction of T-cell proliferation capacity to alloantigen stimuli and a higher proportion of Treg cells. Thus, anti-IL-6 mAb may be protective against acute rejection after cardiac transplantation through suppressing the activation of effector T cells and promoting the induction of Treg cells.
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Expressions of nucleoside diphosphate kinase (nm23) in tumor tissues are related with metastasis and length of survival of patients with hepatocellular carcinoma.
Biomed. Environ. Sci.
PUBLISHED: 06-09-2010
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To evaluate the relationship of expressions of nucleoside diphosphate kinase (nm23) and proliferating cell nuclear antigen (PCNA), as well as apoptosis, with the prognosis of HCC patients by analyzing their pathological and clinical data.
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Application of hepatitis B virus replication mouse model.
World J. Gastroenterol.
PUBLISHED: 04-27-2010
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To evaluate the value of the hepatitis B virus (HBV) replication mouse model with regard to several aspects of the study of HBV biology.
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[New highly phosphorescent heteroleptic tris-cyclometalated iridium (III) complexes: synthesis and photophysical characterization].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 03-23-2010
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New heteroleptic tris-cyclometalated iridium(III) complexes (ppy)2 Ir(LX) (ppy==2-phenylpyridine, LX==Sal (salicylic acid), Msal (4-methylsalicylic acid), FSal(4-trifluoro methyl salicylic acid)) was synthesized and characterized. The molecular structure, photophysical properties and thermal stability were tested and analyzed. The results show that the absorption peaks were located around 270, 370, 450 and 484 nm respectively at room temperature. The two former peaks at 270 and 370 nm should belong to 1pi--pi* transition at ppy and transition from salicylic acid ligands to 2-phenylpyridine; The peaks around 450 and 484 nm can be assigned to the charge transfer transition from Ir to ligand (1MLCT and 3MLCT) and 3pi--pi* transition respectively. The PL emission peaks were located at 520, 522, and 510 nm, respectively. The emission of (ppy), Ir(Sal) and (ppy), Ir (MSal) was mainly ascribed to the radiation transition of triple state 3MLCT, while the emission of (ppy)2 Ir(FSal) was mainly from the radiation transition between Sal and ppy, partly from the radiation transition of single state 1MLCT and triple state 3MLCT. The quantum efficiencies of these complexes were 0.37, 0.33 and 0.29 respectively. The thermal decomposition temperature was from 306 to 328 degrees C. (ppy)2 Ir(LX), being a series of efficient phosphorescent materials with good thermal stability, can be used in the organic electroluminescent devices.
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[Study on the association between DNA double-strand break repair gene NBS1 polymorphisms and susceptibility on lung cancer].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 03-15-2010
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To study the association between DNA double-strand break repair gene NBS1 (nijmegen breakage syndrome gene) polymorphisms and the susceptibility to lung cancer.
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Roles of small GTPase Rac1 in the regulation of actin cytoskeleton during dengue virus infection.
PLoS Negl Trop Dis
PUBLISHED: 02-22-2010
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Increased vascular permeability is a hallmark feature in severe dengue virus (DV) infection, and dysfunction of endothelial cells has been speculated to contribute in the pathogenesis of dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). Rho-family GTPase Rac1 is a significant element of endothelial barrier function regulation and has been implicated in the regulation of actin remodeling and intercellular junction formation. Yet there is little evidence linking Rac1 GTPase to alteration in endothelial cell function induced by DV infection.
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Co-expression of Japanese encephalitis virus prM-E-NS1 antigen with granulocyte-macrophage colony-stimulating factor enhances humoral and anti-virus immunity after DNA vaccination.
Immunol. Lett.
PUBLISHED: 01-11-2010
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Japanese encephalitis virus (JEV) is an agent of Japanese encephalitis, and granulocyte-macrophage colony-stimulating factor (GM-CSF) is an attractive DNA vaccine adjuvant for its antigen presentation. In the present study, we have constructed DNA vaccines that carried JEV prM-E-NS1 genes with or without the GM-CSF gene. Immunization with the bicistronic plasmid pCAG-JEGM that co-expresses GM-CSF and viral prM-E-NS1, resulted in the highest IgG response and sufficient protection against virus-challenged BALB/c mice. However, much to our surprise, co-inoculation of the GM-CSF plasmid with the pCAG-JE plasmid expressing viral prM-E-NS1 lead to a low antibody titer and a relatively low survival rate. Moreover, anamnestic antibody-mediated protection played a dominant role in the mice JEV challenge model, according to the enhancement of post-challenge neutralizing antibody titers and further adoptive transfer experiments. Taken together, this study should encourage further development of JEV DNA vaccine strategies and caution against the use of cytokines as an adjuvant.
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Clinical, histopathological and genetic studies in a family with fatal familial insomnia.
Infect. Genet. Evol.
PUBLISHED: 01-09-2010
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We compared clinical data from two related Chinese patients with fatal familial insomnia (FFI) and collected information about their pedigree. The clinical features in the two cases were similar and included initial progressive insomnia and sympathetic activation, which persisted throughout the clinical course. A total of 135 members of this family, across seven generations, were retrospectively investigated. Eleven family members, including the two FFI cases, were found to have died with similar neurological problems. Analysis of PRNP in 32 family members revealed eleven carrying the D178N allele, including the two FFI patients. Spongiform degeneration in brains was not found, but gliosis was obvious in the thalamus of the two cases at postmortem. Proteinase K-resistant prion protein (PrP) was not found in probands brain by immunohistochemistry, but observed in some areas of brain for both cases by PrP-specific Western blot. Investigation of the pedigree has led to the identification of an additional 9 family members who had similar clinical symptoms and 9 currently healthy individuals with the D178N mutation.
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[The influences of Tet system on activity and tissue specificity of HBV Cp.].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
PUBLISHED: 12-08-2009
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Analyze the effect of Tetracycline-controlled system on Hepatitis B virus core promoter (Cp) activity and tissue specificity.
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[Development of a new FTIR car-borne oil content analyzer].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 12-03-2009
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Turlun structure was introduced into the Fourier transform interference system in the present paper in order to decrease the size and cost of the whole system and to increase the ability of vibration resistance to meet the requirement of car-borne outdoor monitoring application. The speed deviation of the voice coil motor (VCM) driving system, which is based on feedback of interference wavelength of reference laser and DSP control, is within 5%. The standard oil sample was used to test the performance of the spectrometer. The result shows that the highest resolution of the FTIR oil detector is 2 cm(-1), and the SNR reached 2000:1. Compared with old grating spectrometers, thiskind of instrument is faster, and the quality of spectrum was increased significantly. The portable feature of this instrument will be more convenient for the water pollution detection in field
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[Minimally invasive trochanteric antegrade nail fixation for the treatment of intertrochanteric fractures of femur in elder].
Zhongguo Gu Shang
PUBLISHED: 11-12-2009
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To evaluate the clinical effect of minimally invasive Trochanteric Antegrade Nail (TAN) for the treatment of intertrochanteric fractures of femur in elder.
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(Z)-4-[4-(Dimethyl-amino)benzyl-idene]-3-methyl-isoxazol-5(4H)-one.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 10-22-2009
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The title compound, C(13)H(14)N(2)O(2), an isoxazol-5-one derivative, was synthesized by a one-pot, three-component condensation reaction of methyl acetoacetate, hydroxy-lamine hydro-chloride and 4-(dimethyl-amino)benzaldehyde. All the non-H atoms are co-planar [r.m.s deviation = 0.0039?Å], with a Z configuration about the C=C bond. The dihedral angle between the phenyl ring and the isoxazole ring is 2.58?(19)°.
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Cytosolic prion protein induces apoptosis in human neuronal cell SH-SY5Y via mitochondrial disruption pathway.
BMB Rep
PUBLISHED: 08-01-2009
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Different neurodegenerative disorders like prion disease, is caused by protein misfolding conformers. Reverse-transfected cytosolic prion protein (PrP) and PrP expressed in the cytosol have been shown to be neurotoxic. To investigate the possible mechanism of neurotoxicity due to accumulation of PrP in cytosol, a PrP mutant lacking the signal and GPI (CytoPrP) was introduced into the SH-SY5Y cell. MTT and trypan blue assays indicated that the viability of cells expressing CytoPrP was remarkably reduced after treatment of MG-132. Obvious apoptosis phenomena were detected in the cells accumulated with CytoPrP, including loss of mitochondrial transmembrane potential, increase of caspase-3 activity, more annexin V/PI-double positive-stained cells and reduced Bcl-2 level. Moreover, DNA fragmentation and TUNEL assays also revealed clear evidences of late apoptosis in the cells accumulated CytoPrP. These data suggest that the accumulation of CytoPrP in cytoplasm may trigger cell apoptosis, in which mitochondrial relative apoptosis pathway seems to play critical role.
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[Studies on heredity rule of the first genealogy regarding fatal familial insomnia in Henan province].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 07-02-2009
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To investigate the epidemiological, genealogic characteristic, familial history of the families with fatal familial insomnia, its clinical and pathological features as well as the heredity rule of related genes.
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Meta-analysis: adefovir dipivoxil in combination with lamivudine in patients with lamivudine-resistant hepatitis B virus.
Virol. J.
PUBLISHED: 07-01-2009
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Currently, there are no conclusive results on the efficacy of adefovir dipivoxil (ADV) plus lamivudine (LAM) in LAM-resistant patients with chronic hepatitis B (CHB). The aim of study was to evaluate the efficacy of rescue therapy with ADV plus LAM compared to ADV monotherapy in LAM-resistant CHB patients.
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Myosin Vc, a member of the actin motor family associated with Rab8, is involved in the release of DV2 from HepG2 cells.
Intervirology
PUBLISHED: 06-09-2009
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The pathogenesis of the dengue virus (DV) infection has not been well defined. We have reported that actin and Rab8 are involved in DV2 infection. Myosin Vc (Myo5c) is a novel member of the class V myosins and regulates the actin-mediated membrane trafficking associated with Rab8. In this study, the involvement of Myo5c in the release of DV2 was investigated in HpeG2 cells.
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Molecular epidemiological study on prevalence of human papillomaviruses in patients with common warts in Beijing area.
Biomed. Environ. Sci.
PUBLISHED: 05-26-2009
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To study the circulation, distribution, and genomic diversity of HPVs in common warts in Beijing area of China.
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[Analysis of curative effect of discectomy for lumbar intervertebral disc herniation].
Zhongguo Gu Shang
PUBLISHED: 05-05-2009
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To evaluate the short and long term results of discectomy for lumbar intervertebral disc herniation.
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Entry of dengue virus serotype 2 into ECV304 cells depends on clathrin-dependent endocytosis, but not on caveolae-dependent endocytosis.
Can. J. Microbiol.
PUBLISHED: 03-20-2009
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Caveolae- and clathrin-mediated endocytosis are major internalization pathways used by several pathogens; however, their distinctive roles in dengue virus (DV) entry have not been addressed. In this study, we compared the involvement of caveolae- and clathrin-mediated endocytosis in the infectious entry of DV serotype 2 (DV2) into human endothelial-like ECV304 cells. Confocal microscopy study on DV2-infected cells showed that viral antigens were co-localized with clathrin heavy chains, epidermal growth factor pathway substrate clone 15 (Eps15), and adaptin-alpha, but not with caveolin-1. Treatment with chlorpromazine, which inhibits clathrin-dependent endocytosis, led to reduced virus entry into cells, whereas treatment with nystatin, a caveolae inhibitory agent, did not. Furthermore, gene silencing of Eps15 resulted in an average of 75% reduced infection of ECV304 cells by DV2. Our results demonstrated that DV2 enters ECV304 cells by clathrin-dependent endocytosis, not by caveolae-dependent endocytosis.
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Biotransformation of ginsenoside Rf to Rh1 by recombinant beta-glucosidase.
Molecules
PUBLISHED: 03-06-2009
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An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a beta-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (approximately 170 kD) showed the ability to transform ginsenoside Rf into Rh(1).
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Effects of nocturnal light on (clock) gene expression in peripheral organs: a role for the autonomic innervation of the liver.
PLoS ONE
PUBLISHED: 01-22-2009
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The biological clock, located in the hypothalamic suprachiasmatic nucleus (SCN), controls the daily rhythms in physiology and behavior. Early studies demonstrated that light exposure not only affects the phase of the SCN but also the functional activity of peripheral organs. More recently it was shown that the same light stimulus induces immediate changes in clock gene expression in the pineal and adrenal, suggesting a role of peripheral clocks in the organ-specific output. In the present study, we further investigated the immediate effect of nocturnal light exposure on clock genes and metabolism-related genes in different organs of the rat. In addition, we investigated the role of the autonomic nervous system as a possible output pathway of the SCN to modify the activity of the liver after light exposure.
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GM-CSF accelerates proliferation of endothelial progenitor cells from murine bone marrow mononuclear cells in vitro.
Cytokine
PUBLISHED: 01-14-2009
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To test whether the GM-CSF accelerates the proliferation of bone marrow endothelial progenitor cells (BM EPCs).
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Passive protection assay of monoclonal antibodies against dengue virus in suckling mice.
Curr. Microbiol.
PUBLISHED: 01-03-2009
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Dengue fever and dengue hemorrhagic fever/dengue shock syndrome are highly infectious diseases caused by dengue virus (DV). Specific monoclonal antibodies (mAbs) against DV are vital for diagnosis, pathological studies, and passive immune therapy. In this study, purified DV serotype 2 (DV2) was used as antigen and BALB/c mice were immunized to induce specific antibodies. We established five hybridoma cell lines, called 78#, 1E7, 7F7, 8F12, and 8H1, respectively, and evaluated them by enzyme-linked immunosorbent assay, indirect immunofluorescence assay, Western blot, plaque reduction neutralization test, and suckling mice protection assay. Lines 78#, 1E7, 7F7, and 8F12 showed a neutralizing effect, and lines 78#, 1E7, 8F12, and 8H1 recognized envelope glycoprotein of DV2. Among them, lines 78# and 8F12 had stronger neutralizing ability in vitro and could protect some suckling mice from virus challenge. Our results demonstrate that immunization with purified virion is efficient for the production of specific neutralizing mAbs against DV2, and these mAbs could be useful tools for studying or treating DV infection.
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[Association of interleukin 28B polymorphisms with response to pegylatedinterferon plus ribavirin therapy for chronic hepatitis C].
Sichuan Da Xue Xue Bao Yi Xue Ban
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To investigate the characteristics of gene polymorphisms at rs12979860 of interleukin 28B (IL28B) and explore the relationships between the genetic polymorphisms and the antiviral therapy efficiency for chronic hepatitis C patients in the Sichuan region of China.
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[Diffusion tensor imaging analyses of white matter in healthy siblings of schizophrenics].
Zhonghua Yi Xue Za Zhi
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To explore the diffusion tensor imaging (DTI) features of white matter in healthy siblings of schizophrenics.
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A novel fixation system for sacroiliac dislocation fracture: internal fixation system design and biomechanics analysis.
Clin Biomech (Bristol, Avon)
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Although there were many different types of fixation techniques for sacroiliac dislocation fracture, the treat remained challenging in posterior pelvic ring injury. The purpose of this study was to evaluate the biomechanical effects of a novel fixation system we designed.
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Formation of shish-kebabs in injection-molded poly(L-lactic acid) by application of an intense flow field.
ACS Appl Mater Interfaces
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Unlike polyolefins (e.g., isotactic polypropylene), it is still a great challenge to form rich shish-kebabs in biodegradable poly(L-lactic acid) (PLLA) because of its short chain length and semirigid chain backbone. In the present work, a modified injection molding technology, named oscillation shear injection molding, was applied to provide an intense shear flow on PLLA melt in mold cavity, in order to promote shear-induced crystallization of PLLA. Additionally, a small amount of poly(ethylene glycol) (PEG) with flexible chains was introduced for improving the crystallization kinetics. Numerous shish-kebabs of PLLA were achieved in injection-molded PLLA for the first time. High-resolution scanning electronic microscopy and small-angle X-ray scattering showed a structure feature of shish-kebabs with a diameter of around 0.7 ?m and a long period of ~20 nm. The wide-angle X-ray diffraction results showed that shish-kebabs had more ordered crystalline structure of ?-form. A significant improvement of the mechanical properties was obtained; the tensile strength and modulus increased to 73.7 and 1888 MPa from the initial values of 64.9 and 1684 MPa, respectively, meanwhile the ductility is not deteriorated. Interestingly, when shish-kebabs form in the PLLA/PEG system, a bamboo-like bionic structure comprising a hard skin layer and a soft core develops in injection-molded specimen. This unique structure leads to a great balance of mechanical properties, including substantial increments of 26, 20, and 112% in the tensile strength, modulus, and impact toughness, compared to the control sample. Further exploration will give a rich fundamental understanding in the shear-induced crystallization and morphology manipulation of PLLA, aiming to achieve superior PLLA products.
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[Chemical constituents and antibacterial activity contained in Caesalpinia millettii].
Zhongguo Zhong Yao Za Zhi
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To study chemical constituents contained in roots of Caesalpinia millettii by HPLC. Six homoisoflavonoids were identified by spectroscopic data and physicochemical property as eucomin (1), intricatinol (2), 8-methoxybonducellin (3), bonducellin (4), 8-methoxyisobonducellin (5) and 3-(4-methoxybenzyl) -5, 7-dimethoxychroman-4-one (6). All compounds were separated from the root of this genus for the first time. An antibacterial screening was made on eight monomeric compounds. Among them, 8-methoxyisobonducellin, intricatinol, bergenin, hyperoside and 11-O-galloylbergenin showed a inhibitory effect on Staphylococcus aureus, Klebsiella Peneumoniae, Beta streptococcus and Aeruginosus bacillus.
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A comparative proteomics analysis of soybean leaves under biotic and abiotic treatments.
Mol. Biol. Rep.
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A comparative proteomic study was made to explore the molecular mechanisms, which underlie soybean root and stem defense response caused by the oomycete Phytophthora sojae strain P6497. Soybean (Glycine max cv. Xinyixiaoheidou) seedling roots were incubated in salicylic acid, methyl jasmonate, 1-amino cyclopropane-1-carboxylic acid, hydrogen peroxide, sodium nitroprusside, vitamin B(1) and P. sojae zoosperm in order to determine whether the corresponding leaves play a role in the defense response at the proteomic level. The results showed that the proteome of leaves had no significant differences. Of the 21 identified proteins identified in the study, 62 % were involved in predominately in energy functions. Those involved in protein synthesis, secondary metabolism and metabolism categories followed in abundance, where proteins involved as transporters and in transcription were the least and represented only 5 %. Those related to energy were shown to be involved in photosynthesis and photorespiration activities. The present study provides important information with regards to proteomic methods aimed to study protein regulations of the soybean-P. sojae pathosystem, especially in terms of host resistance to this pathogen.
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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.