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Find video protocols related to scientific articles indexed in Pubmed.
The H19/let-7 double-negative feedback loop contributes to glucose metabolism in muscle cells.
Nucleic Acids Res.
PUBLISHED: 11-17-2014
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The H19 lncRNA has been implicated in development and growth control and is associated with human genetic disorders and cancer. Acting as a molecular sponge, H19 inhibits microRNA (miRNA) let-7. Here we report that H19 is significantly decreased in muscle of human subjects with type-2 diabetes and insulin resistant rodents. This decrease leads to increased bioavailability of let-7, causing diminished expression of let-7 targets, which is recapitulated in vitro where H19 depletion results in impaired insulin signaling and decreased glucose uptake. Furthermore, acute hyperinsulinemia downregulates H19, a phenomenon that occurs through PI3K/AKT-dependent phosphorylation of the miRNA processing factor KSRP, which promotes biogenesis of let-7 and its mediated H19 destabilization. Our results reveal a previously undescribed double-negative feedback loop between sponge lncRNA and target miRNA that contributes to glucose regulation in muscle cells.
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Study on the effect of vacuum sealing drainage on the repair process of rabbit sciatic nerve injury.
Int. J. Neurosci.
PUBLISHED: 10-24-2014
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Purpose: To investigate the influence of vacuum sealing drainage on sciatic nerve repair after injury in rabbits. Materials and Methods: Twenty four New Zealand white rabbits were randomly divided into experimental group and control group. About 1 cm sciatic nerve was transected and sutured back in situ. The experimental group had vacuum sealing drainage assisted wound closure whereas the control group had normal wound closure. The nerve repair rate was compared based on nerve conduction velocity, lower leg triceps wet weight recovery rate, histology, immunohistochemical of brain-derived neurotrophic factor, and ultrastructure observation of regenerated nerve by electron microscopy at the 4th and 8th week after surgery. Results: At the 1st-2nd weeks after surgery, irritation and ulcers were observed on the surgical side in both the experimental group and the control group. At the 4th and 8th week after surgery, electrical nerve conduction velocity in the experimental group was faster than in the control group (p < 0.05) and triceps muscle calf wet weight recovery rate in the experimental group was higher than that in the control group (p < 0.05). Brain-derived neurotrophic factor immunohistochemical staining intensity in the experimental group was higher than that in the control group (p < 0.05) and toluidine blue staining and electron microscopic observation showed that the nerve regeneration and repair were more pronounced in the experimental group as compared to the control group. Myelinated nerve fibers in the experimental group were more than that in the control group at the 4th week and 8th week after surgery. Conclusion: Vacuum sealing drainage facilitates repair of peripheral nerve injury.
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[Effects of exhaustive exercise-induced oxidative stress on red blood cell deformability].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
PUBLISHED: 10-22-2014
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The aim of the present study is to explore the effects of exhaustive exercise-induced oxidative stress on the antioxidant capacity and diformability of rat red blood cells.
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Comprehensive review of genetic association studies and meta-analysis on miRNA polymorphisms and rheumatoid arthritis and systemic lupus erythematosus susceptibility.
Hum. Immunol.
PUBLISHED: 09-02-2014
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MicroRNAs (miRNAs), small RNA molecules, play a role in the development and differentiation of immune cells in both innate and adaptive immune responses. Our study was aimed to investigate the association between three miRNA polymorphism and rheumatoid arthritis (RA) or systemic lupus erythematosus (SLE) by using meta-analysis approach.
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Crystal structure of (E)-N'-benzyl-idene-1-methyl-4-nitro-1H-pyrrole-2-carbohydrazide.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 09-01-2014
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In the title compound, C13H12N4O3, the dihedral angle between the planes of the pyrrole and benzene rings is 7.47?(1)°. In the crystal, mol-ecules are arranged in sheets lying parallel to (101). Neighbouring sheets are linked by N-H?O hydrogen bonds, weak ?-? [centroid-centroid distance between the pyrrole rings = 3.765?(11)?Å] and C-H?? inter-actions, forming a three-dimensional structure.
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Metabonomic study of chronic heart failure and effects of Chinese herbal decoction in rats.
J Chromatogr A
PUBLISHED: 08-14-2014
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Shenfu decoction (SFD) is a well-known important traditional Chinese medicine prescription that has been used to treat chronic heart failure (CHF) for many years in China. However, its holistic therapeutic effects and mechanism are not yet well understood. Here, a UPLC/TOF-MS-based metabonomic study was conducted to explore potential biomarkers to increase the understanding of CHF and to assess the integral efficacy of SFD for CHF in rats. Principal component analysis was used to investigate the global metabonomic alterations and to evaluate the therapeutic effects of SFD in rats. Clear separations were observed not only in the comparison of dynamic trajectories but also in the comparison of the metabolite profiles of the normal controls, the model group (CHF), the sham surgery group and the SFD-treated group. The results indicated that SFD exerted therapeutic efficacies for CHF, which were in accordance with the results of the echocardiographic and haemodynamic assay. Furthermore, 16 potential biomarkers from urine samples and 13 potential biomarkers from serum samples of CHF rats were identified and were mainly related to inflammation and to dysfunction of amino acids and energy metabolism. The therapeutic effects of SFD on CHF were partially due to the restoration of these disturbed pathways. This study provides a useful approach for exploring the mechanism of myocardial infarction (MI)-induced CHF and for evaluating the efficacy of SFD on CHF.
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Arterial wall degeneration plus hemodynamic insult cause arterial wall remodeling and nascent aneurysm formation at specific sites in dogs.
J. Neuropathol. Exp. Neurol.
PUBLISHED: 08-12-2014
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To determine whether arterial wall degeneration, in combination with hemodynamic insult, causes cerebral artery aneurysms in a dog model, we simulated the geometry and hemodynamics of a human artery by surgical reconstruction of both common carotid arteries in 12 dogs. The dogs were then randomly assigned to one of the following groups: hemodynamic insult + elastase insult group ( n = 6), hemodynamic insult group (n = 6), or elastase control group (n = 3), in which the straight common carotid arteries were subjected to elastase alone. Angiography and hemodynamic analysis were performed immediately and at 12 weeks after surgery; the animals were then killed for histologic evaluation. The 12 surgically reconstructed distal internal carotid arteries simulated the human artery well with respect to geometric and hemodynamic measurements, with the intended aneurysm sites exposed to higher wall shear stress and velocity, lower pressure, turbulent flow, and changes in wall shear stress gradient. Nascent aneurysms developed in 4 hemodynamic insult + elastase insult group dogs at 12 weeks; blood flow analysis demonstrated decreased wall shear stress, increased pressure, and wall shear stress gradient from the neck to the dome. Arterial wall remodeling or nascent aneurysm formation in the hemodynamic insult + elastase insult group versus the other groups was indicated by internal elastic lamina/elastic fiber disruption, muscular layer thinning, increased smooth muscle cell proliferation, macrophage infiltration, and high expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 in the media. These data suggest that nascent aneurysms were caused by the combination of arterial wall degeneration and hemodynamic perturbations in this distal internal carotid artery dog model.
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[Impact of introduction of O2 on the welding arc of gas pool coupled activating TIG].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 08-07-2014
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In the present paper, Boltzmann plot method was applied to analyze the temperature distributions of the are plasma when the gas pool coupled activating TIG welding was at different coupling degrees with the outer gas being O2. Based on this study of temperature distributions, the changing regularities of are voltage and are appearance were studied. The result shows that compared with traditional TIG welding, the introduction of O2 makes the welding arc constricted slightly, the temperature of the are center build up, and the are voltage increase. When argon being the inner gas, oxygen serving as the outer gas instead of argon makes the are constricted more obviously. When the coupling degree increases from 0 to 2, the temperature of the are center and the are voltage both increase slightly. In the gas pool coupled activating TIG welding the are is constricted not obviously, and the reason why the weld penetration is improved dramatically in the welding of stainless steel is not are constriction.
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Overexpression of activated leukocute cell adhesion molecule in gastric cancer is associated with advanced stages and poor prognosis and miR-9 deregulation.
Mol Med Rep
PUBLISHED: 08-05-2014
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Activated leukocyte cell adhesion molecule (ALCAM) has been identified as a novel potential molecular marker of human tumors. The present study aimed to assess ALCAM as a prognostic marker for gastric cancer (GC), and to explore the mRNA deregulation underlying the abnormal expression of ALCAM. The mRNA and protein expression of ALCAM in GC and adjacent non?tumor tissues from 66 patients with GC were analyzed. The association between miR?9 and ALCAM mRNA expression was determined by quantitative polymerase chain reaction. Serum soluble ALCAM (sALCAM) was analyzed by ELISA in 72 patients with GC, 82 patients with gastric precancerous lesions and 73 controls. ALCAM and sALCAM levels were associated with certain clinicopathological variables, including overall survival. Compared with the non?tumor tissues, the expression of ALCAM mRNA in the GC tissues was significantly upregulated (P=0.013). The expression of miR?9 was reduced and inversely correlated with ALCAM mRNA levels in GC tissues and cell lines. The ALCAM mRNA level was reduced following ectopic overexpression of miR?9 in SGC?7901 human gastric cancer cells. The rates of membranous and cytoplasmic expression of ALCAM in GC tissues were 59.1 and 48.48%, respectively, and the serum sALCAM levels were significantly elevated in patients with GC. Elevated ALCAM mRNA, membranous ALCAM expression in GC tissues and high sALCAM levels are associated with advanced tumor stage, lymphatic invasion and shorter overall survival duration. The results of the current study indicated that membranous ALCAM expression and high serum sALCAM levels are independent prognostic markers of poor survival for patients with GC, and that the overexpression of ALCAM may be due to the downregulation of miR?9.
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Time-effect relationship of extracts from ginseng, notoginseng and chuanxiong on vascular endothelial cells senescence.
Chin J Integr Med
PUBLISHED: 07-30-2014
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To observe the time-effect relation of extracts from ginseng, notoginseng and chuanxiong on angiotensin II (Ang II)-induced senescence of vascular endothelial cells and explore the feature of Chinese medicine against vascular diseases.
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Protective effects of maslinic acid against alcohol-induced acute liver injury in mice.
Food Chem. Toxicol.
PUBLISHED: 07-28-2014
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Protective effects of maslinic acid (MA) at 10, 15 or 20?mg/kg body weight/day against alcohol-induced acute hepatotoxicity in mice were examined. Mice were administrated by MA for 3 weeks, and followed by alcohol treatment. Results showed that MA pre-intake at three doses resulted in its accumulation in the liver; and dose-dependently lowered cytochrome P450 2E1 activity and protein expression at 23.5-51.2% and 21.4-62.3%, respectively (P?<0.05). MA pre-intake decreased subsequent alcohol-induced reactive oxygen species, interleukin-6, tumor necrosis factor-alpha, monocyte chemoattractant protein-1, nitric oxide and prostaglandin E2 production; retained glutathione content; maintained catalase and glutathione peroxidase activities; and declined cyclooxygenase-2 and total nitric oxide synthase activities in the liver (P?<0.05). Furthermore, MA pre-intake suppressed 17.3-51.7% nuclear factor kappa (NF-?)B p50, 23.5-58.8% NF-?B p65, 25.6-62.4% p-p38 and 24.1-63.0% p-JNK expression in the liver (P?<0.05). Histological data indicated that MA intake at test doses attenuated hepatic inflammatory infiltrate. These findings support that maslinic acid is a potent preventive agent against acute alcoholic liver disease.
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Integrating novel chemical weapons and evolutionarily increased competitive ability in success of a tropical invader.
New Phytol.
PUBLISHED: 07-08-2014
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The evolution of increased competitive ability (EICA) hypothesis and the novel weapons hypothesis (NWH) are two non-mutually exclusive mechanisms for exotic plant invasions, but few studies have simultaneously tested these hypotheses. Here we aimed to integrate them in the context of Chromolaena odorata invasion. We conducted two common garden experiments in order to test the EICA hypothesis, and two laboratory experiments in order to test the NWH. In common conditions, C. odorata plants from the nonnative range were better competitors but not larger than plants from the native range, either with or without the experimental manipulation of consumers. Chromolaena odorata plants from the nonnative range were more poorly defended against aboveground herbivores but better defended against soil-borne enemies. Chromolaena odorata plants from the nonnative range produced more odoratin (Eupatorium) (a unique compound of C. odorata with both allelopathic and defensive activities) and elicited stronger allelopathic effects on species native to China, the nonnative range of the invader, than on natives of Mexico, the native range of the invader. Our results suggest that invasive plants may evolve increased competitive ability after being introduced by increasing the production of novel allelochemicals, potentially in response to naïve competitors and new enemy regimes.
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Genome-wide analysis of gestational gene-environment interactions in the developing kidney.
Physiol. Genomics
PUBLISHED: 07-08-2014
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The G protein-coupled bradykinin B2 receptor (Bdkrb2) plays an important role in regulation of blood pressure under conditions of excess salt intake. Our previous work has shown that Bdkrb2 also plays a developmental role since Bdkrb2(-/-) embryos, but not their wild-type or heterozygous littermates, are prone to renal dysgenesis in response to gestational high salt intake. Although impaired terminal differentiation and apoptosis are consistent findings in the Bdkrb2(-/-) mutant kidneys, the developmental pathways downstream of gene-environment interactions leading to the renal phenotype remain unknown. Here, we performed genome-wide transcriptional profiling on embryonic kidneys from salt-stressed Bdkrb2(+/+) and Bdkrb2(-/-) embryos. The results reveal significant alterations in key pathways regulating Wnt signaling, apoptosis, embryonic development, and cell-matrix interactions. In silico analysis reveal that nearly 12% of differentially regulated genes harbor one or more Pax2 DNA-binding sites in their promoter region. Further analysis shows that metanephric kidneys of salt-stressed Bdkrb2(-/-) have a significant downregulation of Pax2 gene expression. This was corroborated in Bdkrb2(-/-);Pax2(GFP+/tg) mice, demonstrating that Pax2 transcriptional activity is significantly repressed by gestational salt-Bdkrb2 interactions. We conclude that gestational gene (Bdkrb2) and environment (salt) interactions cooperate to impact gene expression programs in the developing kidney. Suppression of Pax2 likely contributes to the defects in epithelial survival, growth, and differentiation in salt-stressed BdkrB2(-/-) mice.
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Clinical features of severe cases of hand, foot and mouth disease with EV71 virus infection in China.
Arch Med Sci
PUBLISHED: 06-27-2014
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Hand, foot and mouth disease (HFMD) caused by EV71 infection has become one of the major public health issues in China, which deeply affects children's health. The prevention and control of EV71 is a challenge currently because there is no safe and effective vaccine or antiviral medications available.
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Publication patterns and citation analysis of APJTM during 2008 and June 2014.
Asian Pac J Trop Med
PUBLISHED: 06-19-2014
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To draw on data about publication patterns and citation indicators of Asian Pacific Journal of Tropical Medicine (APJTM) during 2008 and June 2014 in order to know about the current state of the journal.
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Controlled in situ fabrication of Ag2O/AgO thin films by a dry chemical route at room temperature for hybrid solar cells.
Dalton Trans
PUBLISHED: 06-12-2014
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Silver oxides (Ag2O and AgO) have attracted increasing attention as potential solar cell materials for photovoltaic devices due to their ideal bandgap and non-toxicity. In order to eliminate the complicated synthesis and harsh reaction conditions (e.g. high temperature, high vacuum, high energy input, electron beam instrumentation) required by most synthetic strategies, we developed a very facile dry chemical approach to directly prepare AgxO species on the ITO substrate by taking advantage of a UV-O3 surrounding in a controlled way. We systematically investigated the effects of relative humidity, reaction temperature, and the silver deposition technique on the formation of silver oxide (AgO or Ag2O) thin films. A possible synthetic mechanism for the formation of AgO and Ag2O is proposed. More importantly, we have designed and successfully fabricated novel inorganic hybrid Ag2O/Bi2O3 heterojunction thin films for the first time which exhibit significantly improved photocurrent compared with pure Bi2O3 films.
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Oncogenic Fli-1 is a potential prognostic marker for the progression of epithelial ovarian cancer.
BMC Cancer
PUBLISHED: 05-29-2014
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Ovarian cancer is the most lethal gynecologic malignancy, but its etiology remains poorly understood. This study investigated the role of Fli-1 in ovarian carcinogenesis and disease survival.
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Convenient detection of HPV virus in a clinical sample using concurrent rolling circle and junction probe amplifications.
Chem. Commun. (Camb.)
PUBLISHED: 05-24-2014
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Herein we show that two isothermal amplification strategies, rolling circle amplification and junction probe strategy, can be used in tandem in the same tube under isothermal conditions to detect HPV16 in clinical cervical swabs. It was discovered that the prior treatment of the clinical sample with a cocktail of restriction endonucleases (REAses) to digest the genomic DNA facilitated the isothermal detection assay.
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SPRY4-mediated ERK1/2 signaling inhibition abolishes 17?-estradiol-induced cell growth in endometrial adenocarcinoma cell.
Gynecol. Endocrinol.
PUBLISHED: 05-08-2014
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Basic fibroblast growth factor (FGF2)-mediated Extracellular signal-regulated kinases1/2 (ERK1/2) signaling is a critical modulator in angiogenesis. SPRY4 has been reported to be a feedback negative regulator of FGFs-induced ERK1/2 signaling. The aim of this study was to explore the role of SPRY4 in endometrial adenocarcinoma cell.
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Dietary modulation of the expression of genes involved in short-chain fatty acid absorption in the rumen epithelium is related to short-chain fatty acid concentration and pH in the rumen of goats.
J. Dairy Sci.
PUBLISHED: 05-07-2014
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We have tested the hypothesis that increased concentrate intake induces mRNA abundance alterations of genes involved in short-chain fatty acid (SCFA) absorption in the rumen epithelium of goats and these changes of mRNA abundance are probably related to ruminal SCFA concentration and ruminal pH. Goats (n=12) were randomly allocated to 2 groups and fed either a low-concentrate (LC) diet (10% concentrate; n=6) or a medium-concentrate (MC) diet (35% concentrate; n=6) in 2 equal portions daily. Goats were fed separately with their respective diet for 3 wk. The goats were slaughtered 6h after the morning feeding on d 22. In vivo, goats in the MC treatment exhibited a greater ruminal SCFA concentration (81.9mM) compared with those in the LC treatment (58.0mM), and the pH decreased from 7.03 to 6.63. Correspondingly, mRNA expression of candidates for SCFA(-)/HCO3(-) exchangers, namely downregulated in adenoma (DRA), putative anion transporter 1 (PAT1), and anion exchanger 2 (AE2) were increased in the MC group. Further, upregulation in monocarboxylate transporter 1 (MCT1) and monocarboxylate transporter 4 (MCT4) mRNA abundances was observed in the MC group. The expression of genes that help the rumen epithelial cells to maintain intracellular pH, including Na(+)/H(+) exchanger 1 (NHE1), NHE2, NHE3, vacuolar H(+) ATPase subunit B (vH(+)ATPase), and Na(+)/K(+) ATPase pump subunit ?1 (Na(+)/K(+)ATPase) were also enhanced in the MC group relative to the LC group. During in vitro studies with isolated rumen epithelial cells from goats, exposure to a pH of 6.8 increased MCT1 mRNA expression after 24h of culture, whereas the mRNA expression of AE2 was downregulated. The presence of SCFA (20mM) in the medium increased DRA, PAT1, AE2, MCT1, and Na(+)/K(+)ATPase mRNA expression. The expression of vH(+)ATPase was decreased by increased SCFA concentration. The mRNA expression of MCT4 did not vary in vitro with pH (6.8) or SCFA (20mM). The expression of DRA was increased by synergistic effects of higher SCFA concentration and lower pH, similar to that of MCT1. Thus, diet-dependent rumen epithelial mRNA expression changes in genes involved in SCFA absorption are probably related to ruminal SCFA concentration and pH.
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Asiatic acid ameliorates hepatic lipid accumulation and insulin resistance in mice consuming a high-fat diet.
J. Agric. Food Chem.
PUBLISHED: 05-06-2014
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Effects of asiatic acid (AA) at 10 or 20 mg/kg/day upon hepatic steatosis in mice consuming a high-fat diet (HFD) were examined. AA intake decreased body weight, water intake, feed intake, epididymal fat, and plasma and hepatic triglyceride levels in HFD-treated mice (P < 0.05). HFD enhanced 2.85-fold acetyl coenzyme A carboxylase (ACC1), 3.34-fold fatty acid synthase (FAS), 3.71-fold stearoyl CoA desaturase (SCD)-1, 3.62-fold 3-hydroxy-3-methylglutaryl coenzyme A reductase, 2.91-fold sterol regulatory element-binding protein (SREBP)-1c, and 2.75-fold SREBP-2 expression in liver (P < 0.05). Compared with HFD groups, AA intake at two doses reduced 18.9-45.7% ACC1, 25.1-49.8% FAS, 24.7-57.1% SCD-1, and 21.8-53.3% SREBP-1c protein expression (P < 0.05). Histological results indicated AA intake at two doses reduced hepatic lipid accumulation and inflammatory infiltrate. HFD increased hepatic production of reactive oxygen species, interleukin (IL)-1?, IL-6, and tumor necrosis factor-?, as well as decreased hepatic glutathione content and glutathione peroxidase and catalase activities (P < 0.05). AA intake at two doses reversed these alterations (P < 0.05). AA intake suppressed 32.4-58.8% nuclear factor kappa (NF-?)B p65 and 24.2-56.7% p-p38 expression (P < 0.05) and at high dose down-regulated 29.1% NF-?B p50 and 40.7% p-JNK expression in livers from HFD-treated mice. AA intake at two doses lowered plasma insulin secretion and HOMR-IR (P < 0.05). These results suggest that AA is a potent hepatic protective agent against HFD-induced hepatic injury.
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High concentrate:forage ratio diet inhibiting omasal epithelium growth is associated with decreased cyclin D1 and CDK4 expression in growing goats.
Anim. Sci. J.
PUBLISHED: 04-15-2014
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The hypothesis that different concentrate?:?forage ratio diets alter omasal epithelium proliferation of growing goats via cyclins and regulation of the cell cycle was tested. Growing goats were fed with a high concentrate (HC, n?=?8) or a low concentrate (LC, n?=?8) diet for 42 days. The concentrate?:?forage ratio was 40:60 in the HC group and 0:100 in the LC group. In the HC group, the relative weight and DNA content of the omasal epithelium were lower, but the protein?:?DNA ratio was higher. Flow cytometry revealed that HC omasal cell numbers were smaller in S- and G2 /M-phases of the cell cycle and higher in the G0 /G1 -phases and were accompanied by reduced expression of cyclin D1 and CDK4 mRNA and protein. These data are consistent with morphologic observations in the HC that cell density decreased in the stratum spinosum (SS) plus stratum granulosum (SG) and stratum basale, and that cell density was lower in the SS plus SG. Thus, high-concentrate?:?forage ratio diet retards omasal epithelial growth by slowing the G1 to S phase transition of the cell cycle and is associated with decreased cyclin D1 and CDK4 expression in growing goats.
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Isothermal amplified detection of DNA and RNA.
Mol Biosyst
PUBLISHED: 03-20-2014
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This review highlights various methods that can be used for a sensitive detection of nucleic acids without using thermal cycling procedures, as is done in PCR or LCR. Topics included are nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), loop-mediated amplification (LAMP), Invader assay, rolling circle amplification (RCA), signal mediated amplification of RNA technology (SMART), helicase-dependent amplification (HDA), recombinase polymerase amplification (RPA), nicking endonuclease signal amplification (NESA) and nicking endonuclease assisted nanoparticle activation (NENNA), exonuclease-aided target recycling, Junction or Y-probes, split DNAZyme and deoxyribozyme amplification strategies, template-directed chemical reactions that lead to amplified signals, non-covalent DNA catalytic reactions, hybridization chain reactions (HCR) and detection via the self-assembly of DNA probes to give supramolecular structures. The majority of these isothermal amplification methods can detect DNA or RNA in complex biological matrices and have great potential for use at point-of-care.
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A benzothiadiazole-cyclopentadithiophene [corrected] bridged D-A-?-A sensitizer with enhanced light absorption for high efficiency dye-sensitized solar cells.
Chem. Commun. (Camb.)
PUBLISHED: 03-07-2014
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We demonstrate an efficient D-A-?-A sensitizer with a benzothiadiazole-cyclopentadithiophene [corrected] moiety as the spacer in a triphenylamine organic dye for dye-sensitized solar cells. The dye has a broad visible light absorption range up to 800 nm. A power conversion efficiency >9% has been achieved using a [Co(bpy)3](2+/3+)-based electrolyte.
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Panax notoginseng saponins inhibits atherosclerotic plaque angiogenesis by down-regulating vascular endothelial growth factor and nicotinamide adenine dinucleotide phosphate oxidase subunit 4 expression.
Chin J Integr Med
PUBLISHED: 03-06-2014
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To investigate the mechanism of Panax notoginseng saponins (PNS), an effective component extracted from Panax notoginseng, on atherosclerotic plaque angiogenesis in atherosclerosis-prone apolipoprotein E-knockout (ApoE-KO) mice fed with high-fat, high-cholesterol diet.
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Elevation of ceramide and activation of secretory acid sphingomyelinase in patients with acute coronary syndromes.
Coron. Artery Dis.
PUBLISHED: 03-05-2014
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Although there are several reported evidences for a pathogenic role of sphingolipid signaling in atherosclerosis, peripheral blood levels of ceramide and secretory acid sphingomyelinase (S-SMase) activity in patients with acute coronary syndromes (ACS) have not been evaluated.
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Fungal invasion of epithelial cells.
Microbiol. Res.
PUBLISHED: 02-23-2014
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Interaction between host cells and invasive Candida plays a large role in the pathogenicity of Candida species. Fungal-induced endocytosis and active penetration are the two distinct, yet complementary invasion mechanisms of invasive candidiasis. Induced endocytosis is a microorganism-triggered, epithelial-driven, clathrin-mediated and actin-dependent process. During the fundamental pathological process of induced endocytosis, invasins (Als3 and Ssa1), which mediate the binding of host epithelial surface proteins, are expressed by Candida species on the hyphal surface. Sequentially, the interaction between invasins and host epithelial surface proteins stimulates the recruitment of clathrin, dynamin and cortactin to the sites where Candida enters epithelial cells, which in turn induce the actin cytoskeleton reorganization. Actin cytoskeleton provides the force required for fungal internalization. Parallely, active penetration of Candida can directly pass through epithelial cells possibly due to progressive elongation of hyphae and physical forces. Several molecules, such as secreted hydrolases and Als3, can affect the protective barrier of the epithelium and make Candida actively penetrate into epithelial cells through intercellular gaps of epithelial layers.
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Platelets promote tumour metastasis via interaction between TLR4 and tumour cell-released high-mobility group box1 protein.
Nat Commun
PUBLISHED: 02-17-2014
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Increasing evidence suggests that TLR4 expression by tumour cells promotes tumour progression, but it is unclear whether TLR4 is involved in metastasis. Here we show that TLR4 deficiency significantly diminishes experimental lung metastasis without affecting primary tumour growth. Bone marrow transplantation experiment and application of antiplatelet agents in mice demonstrate that TLR4 on platelets plays an important role in metastasis. TLR4 is critical for platelet-tumour cell interaction in vitro. Furthermore, high-mobility group box1 (HMGB1) neutralization attenuates platelet-tumour cell interaction in vitro and metastasis in vivo in a TLR4-dependent manner, indicating that tumour cell-released HMGB1 is the key factor that interacts with TLR4 on platelets and mediates platelet-tumour cell interaction, which promotes metastasis. These findings demonstrate a mechanism by which platelets promote tumour cell metastasis and suggest TLR4, and its endogenous ligand HMGB1 as targets for antimetastatic therapies.
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Draft Genome Sequence of Bacillus subtilis Type Strain B7-S, Which Converts Ferulic Acid to Vanillin.
Genome Announc
PUBLISHED: 02-15-2014
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The Bacillus subtilis type strain B7-S was obtained through induction with ferulic acid. Here, we present the draft genome of strain B7-S, which contains 5,313,924 bp, with a G+C content of 35.8%, 5,135 protein-coding genes, and 40 tRNA-encoding genes.
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Effect of fibroids not distorting the endometrial cavity on the outcome of in vitro fertilization treatment: a retrospective cohort study.
Fertil. Steril.
PUBLISHED: 01-11-2014
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To investigate the effect of fibroids that do not distort the endometrial cavity on IVF/intracytoplasmic sperm injection (ICSI) outcomes and to identify certain fibroid subgroups that may be deleterious to fertility outcomes.
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Andrographolide suppress tumor growth by inhibiting TLR4/NF-?B signaling activation in insulinoma.
Int. J. Biol. Sci.
PUBLISHED: 01-01-2014
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Insulinomas are rare tumors, and approximately 10% of insulinomas are malignant. Accumulating evidence has implicated that we still lack effective therapy to treat the patients who are diagnosed with rare malignant insulinoma. Previous studies have reported that Andrographolide (Andro) could inhibit cell cycle progression, reduce cell invasion and induce cell apoptosis in many common cancer cells. However, the effects of andro are cell type-dependent. So we emplored the ?-TC-6 cells and the RIP1-Tag2 transgenic mouse model of endogenously growing insulinoma model to elucidate the possible anti-cancer effect of Andro on insulinoma, an uncommon type of malignant cancers in this study. Our experiments revealed that Andro significantly inhibited tumor growth at both the early-stage and the advanced-stage of insulinoma through targeting the TLR4/NF-?B signaling pathway. This work initially provides the evidence that the TLR4/NF-?B signaling pathway might be vital as a potential therapeutic target, and also indispensable in Andro-mediated anti-cancer effect in insulinoma.
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A regulatory effect of INMAP on centromere proteins: antisense INMAP induces CENP-B variation and centromeric halo.
PLoS ONE
PUBLISHED: 01-01-2014
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CENP-B is a highly conserved protein that facilitates the assembly of specific centromere structures both in interphase nuclei and on mitotic chromosomes. INMAP is a conserved protein that localizes at nucleus in interphase cells and at mitotic apparatus in mitotic cells. Our previous results showed that INMAP over-expression leads to spindle defects, mitotic arrest and formation of polycentrosomal and multinuclear cells, indicating that INMAP may modulate the function of (a) key protein(s) in mitotic apparatus. In this study, we demonstrate that INMAP interacts with CENP-B and promotes cleavage of the N-terminal DNA binding domain from CENP-B. The cleaved CENP-B cannot associate with centromeres and thus lose its centromere-related functions. Consistent with these results, CENP-B in INMAP knockdown cells becomes more diffused around kinetochores. Although INMAP knockdown cells do not exhibit gross defects in mitotic spindle formation, these cells go through mitosis, especially prophase and metaphase, with different relative timing, indicating subtle abnormality. These results identify INMAP as a model regulator of CENP-B and support the notion that INMAP regulates mitosis through modulating CENP-B-mediated centromere organization.
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Airborne validation of ground-object detection from polarized neutral-point atmosphere.
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 12-28-2013
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Based on the objects polarization effects, polarization is a newly emerging method in the field of remote sensing. Both objects and atmosphere have polarization effects, however, the atmospheres polarization effects are much stronger than that of objects. Consequently, atmosphere polarization effects will interfere or even cover objects when observing with sensors. How to maximally eliminate the polarized effects generated by the atmosphere is a crucial problem in polarization remote sensing. Atmospheric neutral point is an area where the degree of atmosphere polarization is near to zero; therefore, if sensors are set up in this area, atmosphere polarization would be greatly eliminated, which is the main content of separating the effects between objects and atmosphere by its neutral point method. In this paper, after processing and analyzing the experimental data got from the first polarization remote sensing flight experiment with atmosphere neutral point, the degree of polarization images captured in neutral and non-neutral point area were obtained, and it can be seen that the main value of polarized degree of images got in neutral point area was obviously smaller than that in non-neutral point area. The results showed that the theory mentioned above was logical and practical. An innovation in our study is that the requirements needed in polarization remote sensing flight with neutral point were clarified. In the meantime, a qualitative conclusion was drawn that observing with longer wavelength is more applicable to polarization remote sensing.
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Effect and mechanism of Qishen Yiqi Pills on adriamycin- induced cardiomyopathy in mice.
Chin J Nat Med
PUBLISHED: 12-24-2013
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To study the effect and probable mechanism of Qishen Yiqi Pills on adriamycin (ADR)-induced cardiomyopathy in mice.
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Procalcitonin guided antibiotic therapy of acute exacerbations of asthma: a randomized controlled trial.
BMC Infect. Dis.
PUBLISHED: 12-09-2013
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This randomized controlled trial aimed to evaluate whether the serum procalcitonin (PCT) level can be utilized to guide the use of antibiotics in the treatment of acute exacerbations of asthma.
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Outcomes of antiplatelet therapy for haemorrhage patients after thrombolysis: a prospective study based on susceptibility-weighted imaging.
Radiol Med
PUBLISHED: 12-03-2013
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The authors evaluated the effect of susceptibility-weighted imaging (SWI) for antiplatelet therapy on post-thrombolysis microbleeds (MB).
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[Effect of HIF-1alpha on sex hormone levels and germ cell apoptosis of mice].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
PUBLISHED: 11-02-2013
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To study the effect of hypoxia on hypothalamus-adenohypophysis-testis axis hormone levels, germ cell apoptosis and hypoxia-inducible factor-1alpha (HIF-1alpha) expression in testis of adolescent mice, and explore HIF-1alpha regulation on the reproductive function of male mice.
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[Central wavelength shift analysis between laboratory and field spectral calibrations of grating based imaging spectrometer].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-29-2013
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Spectral calibration must be carried out in order to determine its central wavelength and half-wave band width of each pixel before the usage of imaging spectrometer. But it was found out that these parameters vary as environment changes. The present paper studies the effect based on test field data. The authors analyzed the optical structure and compared the working environmental parameters. Then a theoretical model is established and the influences of vibration, distortion and temperature parameters are evaluated. The theoretical model and the caculation results are in good consistency, which testifies the theoretical model. This research will shed some light on the high accuracy spectral calibration of the grating based imaging spectrometer and its manufacture.
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Inhibition of tumor growth and metastasis in non-small cell lung cancer by LY2801653, an inhibitor of several oncokinases, including MET.
Clin. Cancer Res.
PUBLISHED: 08-29-2013
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Lung cancer is the leading cause of cancer-related death worldwide. Sustained activation, overexpression, or mutation of the MET pathway is associated with a poor prognosis in a variety of tumors, including non-small cell lung cancer (NSCLC), implicating the MET pathway as a potential therapeutic target for lung cancer. Previously, we reported on the development of LY2801653: a novel, orally bioavailable oncokinase inhibitor with MET as one of its targets. Here, we discuss the evaluation of LY2801653 in both preclinical in vitro and in vivo NSCLC models. Experimental Design/
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Qindan capsule changes adventitial collagen synthesis in spontaneously hypertensive rats.
Chin J Integr Med
PUBLISHED: 08-24-2013
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To investigate the effect of Qindan capsule (QC) on collagen synthesis and the mechanism underlying the process in spontaneously hypertensive rats (SHRs).
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Geometric, hemodynamic, and pathological study of a distal internal carotid artery aneurysm model in dogs.
Stroke
PUBLISHED: 08-08-2013
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We created a distal internal carotid artery side-wall aneurysm model in dogs and compared its geometric, hemodynamic, and histological similarities with human models.
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Upregulation of nucleus HDGF predicts poor prognostic outcome in patients with penile squamous cell carcinoma bypass VEGF-A and Ki-67.
Med. Oncol.
PUBLISHED: 07-21-2013
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Hepatoma-derived growth factor (HDGF) has been verified to serve as a credible prognostic marker for several types of cancers, but its role in urologic carcinomas remains undetermined. In this study, we analyzed the significance of HDGF, as well as its relative factors such as vascular endothelial growth factor-A (VEGF-A) and Ki-67, in penile squamous cell carcinoma (PSCC). Formalin-fixed paraffin-embedded PSCC samples from 54 patients receiving surgery at Qilu Hospital of Shandong University were included in the retrospective study. The expressions of HDGF, VEGF-A, and Ki-67 were detected by immunohistochemistry of a non-biotin polymerized horseradish peroxidase method. The relationships between the expressions of HDGF and VEGF-A, Ki-67 were assessed. Moreover, their correlations with clinical pathologic characteristics and disease prognosis were, respectively, evaluated. HDGF, VEGF-A, and Ki-67 were positively expressed in 28 (51.9%), 29 (53.7%), and 26 (48.1%) patients, respectively. The expressions of VEGF-A and Ki-67 were closely correlated with PSCC type (P < 0.05). A statistically significant relationship between the expressions of HDGF and VEGF-A in PSCC was observed (P = 0.03). Patients with symptom interval of more than 6 months had a significantly poorer survival rate than those with symptom interval less than 6 months (43.3 vs. 70.8%, P = 0.043). Patients with positive HDGF expression also showed a significantly poorer survival rate than those with negative HDGF expression (39.3 vs. 73.1%, P = 0.013). Logistic regression demonstrated that the expression level of HDGF was an independent predictor for the prognosis of postoperative patients. The expression of HDGF significantly correlated with VEGF-A, but not Ki-67 expression. Overexpression of HDGF, rather than VEGF-A or Ki-67, was confirmed to be an independent prognosticator of poor outcome for PSCC patients.
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Simultaneous analysis of polygala acid, senegenin and 3,6-disinapoylsucrose in rat plasma by liquid chromatography-tandem mass spectrometry: application to a pharmacokinetic study after oral administration.
Biomed. Chromatogr.
PUBLISHED: 07-12-2013
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A rapid and specific LC-MS/MS method has been developed for the simultaneous analysis of polygala acid, senegenin and 3,6-disinapoylsucrose (DSS) in rat plasma. The method was applied to the pharmacokinetics studies of polygala acid, senegenin and DSS. The analysis was carried out on an Agilent Eclipse plus C18 reversed-phase column (100?×?4.6 mm, 3.5 µm) by gradient elution with methanol and ammonia (0.01%, v/v). The flow rate was 0.4 mL/min. All analytes including internal standard (IS) were monitored by selected reaction monitoring with an electrospray ionization source. Linear responses were obtained for polygala acid and DSS ranging from 2.5 to 2000 ng/mL, and senegenin ranging from 5 to 2000 ng/mL. The intra- and inter-day precisions (relative standard deviation) were <11.34 and 8.99%. The extraction recovery ranged from 70.89?±?4.60 to 88.49?±?3.26%, and that for the IS was 77.23?±?3.68%. Stability studies showed that polygala acid, senegenin and DSS are stable during the preparation and analytical process. The validated method was successfully used to determine the concentration-time profiles of polygala acid, senegenin and DSS. Copyright © 2013 John Wiley & Sons, Ltd.
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Alteration of Th1/Th2/Th17 cytokine profile and humoral immune responses associated with chromate exposure.
Occup Environ Med
PUBLISHED: 06-27-2013
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The role of chromate exposure in the deregulation of total lymphocyte and other immune factors is largely unclear.
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Rosiglitazone alleviates injury in rats with adenine?induced chronic kidney disease.
Mol Med Rep
PUBLISHED: 05-29-2013
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Rosiglitazone (ROG) has been shown to exert beneficial effects on glycemic control and renal protection. Circulatory fibroblast growth factor?23 (FGF?23) is a novel independent risk factor for chronic kidney disease (CKD) progression. The current study focused on how ROG impacts on injury of the kidney, and whether FGF?23 is involved in the process. A total of 24 male Sprague Dawley rats, weighing 250?280 g, were divided into four groups (n=6 per group): i) Normal; ii) ROG controls, treated with ROG (10 mg/kg/day); iii) CKD models, treated with adenine (200 mg/kg/day); and iv) ROG treatment, treated with ROG (10 mg/kg/day) and adenine (200 mg/kg/day). The rats were sacrificed after four weeks, and serum, urine and kidney tissues were collected. The data revealed that the serum levels of inorganic phosphate (Pi), intact parathyroid hormone (iPTH) and FGF?23 were significantly higher in the CKD models compared with those in the normal group (P<0.01), while ROG significantly reduced the serum levels of Pi, iPTH and FGF?23 (P<0.01). The ratio of protein/creatine (Cr) in the urine and the serum levels of Cr, blood urea nitrogen and uric acid were significantly increased in the CKD models compared with the normal group (P<0.01), however, ROG significantly reduced these parameters (P<0.05). Furthermore, ROG significantly alleviated the tubule interstitial damage index of the CKD models in comparison with that of the controls. These results indicated that ROG mitigates the lesions of chronic kidney disease induced by adenine, and that the system of Pi?PTH?FGF?23 may contribute to this process. The possible mechanisms underlying this process require confirmation in future studies.
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[Multi-angular polarized characteristics of ocean aerosol].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 05-28-2013
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Ocean aerosol is an important component of troposphere aerosol, playing an important role in the global radiative balance and global climate change. To evaluate the direct and indirect radiative effects of aerosol needs deep research on aerosol properties. Multi-angular polarization provides a new method to retrieve aerosol optic and microphysical properties of aerosol. On the basis of the research on optic properties of ocean aerosol at the wavelength of 550 and 860 nm, a vector radiative transfer model was used to simulate the reflectance and polarized reflectance at the top of atmosphere. The sensitivity of reflectance and polarized reflectance to underlying surface, viewing azimuth and aerosol optic depth are evaluated. The simulation results show that the multi-angular polarization information could effectively provide information about the ocean aerosol, which suggests a useful way for ocean aerosol retrieval. Based on the studies of sensitivity, the theory of using multi-angular polarized remote sensing data to retrieve aerosol information is proposed.
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Effect of Adenomyosis on In Vitro Fertilization/Intracytoplasmic Sperm Injection Outcomes in Infertile Women: A Retrospective Cohort Study.
Gynecol. Obstet. Invest.
PUBLISHED: 05-27-2013
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Aims: To study the effect of adenomyosis on in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) outcomes in infertile patients. Methods: We performed a retrospective, database-searched cohort study based on 10,268 patients undergoing controlled ovarian hyperstimulation and IVF/ICSI between 2009 and 2011 in our unit. Adenomyosis was diagnosed by transvaginal ultrasound. A high-quality matched cohort study with strict inclusion criteria was conducted. We compared the basic characteristics and main IVF/ICSI outcomes between the two groups. Results: We identified 83 patients with adenomyosis, of whom we included 77, and strictly matched them to 77 patients without adenomyosis. Higher day 3 estrogen levels and a longer duration of gonadotropin stimulation days were found in women with adenomyosis compared to control subjects. Patients with adenomyosis had a nonsignificant trend toward a lower clinical pregnancy rate and a higher miscarriage rate (p = 0.103 and 0.09, respectively). The delivery rate was significantly lower in the adenomyosis group in comparison to the matched controls (p = 0.022). Conclusions: Within the limitations of a retrospective study (albeit with a remarkably large number of observations), our results suggest that transvaginal ultrasound-diagnosed adenomyosis could have a negative impact on the main IVF/ICSI outcomes. Improving the diagnostic validity and scoring of disease severity in patients with adenomyosis is suggested. © 2013 S. Karger AG, Basel.
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[Spectral calibration of hyperspectral imager based on spectral absorption target].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 05-24-2013
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Retrieval of center wavelength and bandwidth is a key step for quantitative analysis of hyperspectral data. The present paper proposes a spectral calibration method of hyperspectral imager, whose spectrum covers visible and near-infrared band, using spectral absorption target. Ground calibration experiment was designed for a hyperspectral imager with a bandwidth of 6 nm. Hyperspectral imager and ASD spectrometer measured the same spectral absorption target synchronously. Reflectance spectrum was derived from the different data set. Center wavelength and bandwidth were retrieved by matching the reflectance data from hyperspectral imager and ASD spectrometer. The experiment result shows that this method can be applied in spectral calibration of hyperspectral imagers to improve the quantitative studies on hyperspectral imagery.
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[Influence of sensor spectral parameters on the simulation of hypespectral data based on the spectral reconstruction approach].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 05-24-2013
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Data simulation has been widely used in various applications of remote sensing, especially in design of new type sensors, test of new developed algorithms and other associated applications. However, change of parameters of sensor and its system can affect the accuracy of data simulations. Based on spectral reconstruction, the present study employs convolution of spectral response function (SRF) of four bands-blue, green, red, and infrared red within the wide field of view multispectral imager to analyze the impact central wavelength and bandwidth have on the accuracy of spectral reconstruction. The results show that root mean square error (RMSE) caused by central wavelength displacement is less than 0.025, while RMSE caused by bandwidth shift is less than 0.012, indicating the good accuracy of data simulations. Apparently, central wavelength and bandwidth have impact on accuracy of spectral reconstruction to some extent. Therefore, hyperspectral reconstruction depending on central wavelength and bandwidth is conducive for users to further understand hyperspectral imaging system, to find the main factor(s) influencing system performance, to better simulate hyperspectral data and to broaden the application range of remotely sensed data.
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The imprinted H19 lncRNA antagonizes let-7 microRNAs.
Mol. Cell
PUBLISHED: 05-22-2013
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Abundantly expressed in fetal tissues and adult muscle, the developmentally regulated H19 long noncoding RNA (lncRNA) has been implicated in human genetic disorders and cancer. However, how H19 acts to regulate gene function has remained enigmatic, despite the recent implication of its encoded miR-675 in limiting placental growth. We noted that vertebrate H19 harbors both canonical and noncanonical binding sites for the let-7 family of microRNAs, which plays important roles in development, cancer, and metabolism. Using H19 knockdown and overexpression, combined with in vivo crosslinking and genome-wide transcriptome analysis, we demonstrate that H19 modulates let-7 availability by acting as a molecular sponge. The physiological significance of this interaction is highlighted in cultures in which H19 depletion causes precocious muscle differentiation, a phenotype recapitulated by let-7 overexpression. Our results reveal an unexpected mode of action of H19 and identify this lncRNA as an important regulator of the major let-7 family of microRNAs.
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[Pedicled superior gluteal artery perforator bilateral quadrilobed flaps for repair of large sacrococcygeal pressure sores].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
PUBLISHED: 05-16-2013
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To investigate the effectiveness of pedicled superior gluteal artery perforator bilateral quadrilobed flaps for repairing large sacrococcygeal pressure sores.
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Evolutionary increases in defense during a biological invasion.
Oecologia
PUBLISHED: 05-07-2013
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Invasive plants generally escape from specialist herbivores of their native ranges but may experience serious damage from generalists. As a result, invasive plants may evolve increased resistance to generalists and tolerance to damage. To test these hypotheses, we carried out a common garden experiment comparing 15 invasive populations with 13 native populations of Chromolaena odorata, including putative source populations identified with molecular methods and binary choice feeding experiments using three generalist herbivores. Plants from invasive populations of C. odorata had both higher resistance to three generalists and higher tolerance to simulated herbivory (shoot removal) than plants from native populations. The higher resistance of plants from invasive populations was associated with higher leaf C content and densities of leaf trichomes and glandular scales, and lower leaf N and water contents. Growth costs were detected for tolerance but not for resistance, and plants from invasive populations of C. odorata showed lower growth costs of tolerance. Our results suggest that invasive plants may evolve to increase both resistance to generalists and tolerance to damage in introduced ranges, especially when the defense traits have low or no fitness costs. Greater defenses in invasive populations may facilitate invasion by C. odorata by reducing generalist impacts and increasing compensatory growth after damage has occurred.
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Magnetic properties of Acidithiobacillus ferrooxidans.
Mater Sci Eng C Mater Biol Appl
PUBLISHED: 05-01-2013
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Understanding the magnetic properties of magnetotactic bacteria (MTBs) is of great interest in fields of life sciences, geosciences, biomineralization, biomagnetism, and planetary sciences. Acidithiobacillus ferrooxidans (At. ferrooxidans), obtaining energy through the oxidation of ferrous iron and various reduced inorganic sulfur compounds, can synthesize intracellular magnetite magnetosomes. However, the magnetic properties of such microorganism remain unknown. Here we used transmission electronmicroscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) assay, vibrating sample magnetometer (VSM), magneto-thermogravimetric analysis (MTGA), and low temperature magnetometry to comprehensively investigate the magnetic characteristics of At. ferrooxidans. Results revealed that each cell contained only 1 to 3 magnetite magnetosomes, which were arranged irregularly. The magnetosomes were generally in a stable single-domain (SD) state, but superparamagnetic (SP) magnetite particles were also found. The calcined bacteria exhibited a ferromagnetic behavior with a Curie Temperature of 454 °C and a coercivity of 16.36 mT. Additionally, the low delta ratio (?FC/?ZFC=1.27) indicated that there were no intact magnetosome chains in At. ferrooxidans. Our results provided the new insights on the biomineralization of bacterial magnetosomes and magnetic properties of At. ferrooxidans.
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New method for retrospective study of hemodynamic changes before and after aneurysm formation in patients with ruptured or unruptured aneurysms.
BMC Neurol
PUBLISHED: 04-27-2013
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Prospective observation of hemodynamic changes before and after formation of brain aneurysms is often difficult. We used a vessel surface repair method to carry out a retrospective hemodynamic study before and after aneurysm formation in a ruptured aneurysms of the posterior communicating artery (RPcomAA) and an unruptured aneurysms of the posterior communicating artery (URPcomAA).
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Application of the Willis covered stent using the telescopic technique for the treatment of fusiform aneurysm in a canine model.
Neurol India
PUBLISHED: 03-08-2013
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Background: The covered stent is one of the most promising tools for the treatment of intracranial fusiform aneurysms. We developed an in vivo model of fusiform aneurysms and evaluated the effectiveness of double telescoping Willis covered stents for their treatment. Materials and Methods: An external jugular vein graft was anastomosed with the common carotid artery (CCA) to construct the fusiform aneurysm model. After at least 4 weeks, two Willis covered stents were implanted in a telescopic fashion. Angiography follow-up was performed at 2 weeks and 1 and 3 months to examine the grafts. The animals were sacrificed at 1 or 3 months of the follow-up period, and the stents were examined histologically. Results: A total of eight fusiform aneurysms in four canines were created and 16 covered stents were implanted successfully. No technical or device-related difficulties occurred. The angiographic follow-up results showed that six fusiform aneurysms were completely occluded, and a minimal endoleak occurred in two fusiform aneurysms. Histological examination revealed endothelial progress and all aneurysm sacs were filled with thrombi. Conclusion: Vein graft anastomosis with CCA to construct a model of fusiform aneurysm may reproduce the clinical conditions. This study demonstrated that the implantation of two Willis covered stents in a telescoping fashion is an effective way to treat an experimental model of fusiform aneurysm.
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Vector Monte Carlo simulations on atmospheric scattering of polarization qubits.
J Opt Soc Am A Opt Image Sci Vis
PUBLISHED: 03-05-2013
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In this paper, a vector Monte Carlo (MC) method is proposed to study the influence of atmospheric scattering on polarization qubits for satellite-based quantum communication. The vector MC method utilizes a transmittance method to solve the photon free path for an inhomogeneous atmosphere and random number sampling to determine whether the type of scattering is aerosol scattering or molecule scattering. Simulations are performed for downlink and uplink. The degrees and the rotations of polarization are qualitatively and quantitatively obtained, which agree well with the measured results in the previous experiments. The results show that polarization qubits are well preserved in the downlink and uplink, while the number of received single photons is less than half of the total transmitted single photons for both links. Moreover, our vector MC method can be applied for the scattering of polarized light in other inhomogeneous random media.
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Expression of survivin and its four splice variants in colorectal cancer and its clinical significances.
Med. Oncol.
PUBLISHED: 02-13-2013
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The aim of this study was to investigate the clinical significances of the mRNA expression of survivin gene and its four splice variants in the pathogenesis of colorectal cancer (CRC). CRC samples, matched adjacent tissues, and normal tissues were collected from surgical resections of 39 patients with histologically confirmed diagnosis. The mRNA expression of survivin and its four splice variants, that is, survivin-?Ex3, survivin-2B, survivin-3B, and survivin-2?, was detected using semiquantitative PCR and RT-PCR. Carcinoembryonic antigen (CEA) CAM5 was determined as control. The mRNA expression rates of survivin, survivin-?Ex3, survivin-2B, survivin-3B, surviving-2?, and CEA CAM5 in CRC samples were significantly higher than those in adjacent tissues (P < 0.01) and those in normal tissues (P < 0.01). The mRNA levels of the above variants in CRC samples were also significantly higher than those in adjacent tissues (P < 0.01) and those in normal tissues (P < 0.01). The mRNA levels of survivin, survivin-2B, and survivin-2? were not associated with any clinical variable of patients, while the levels of survivin-?Ex3 and survivin-3B were associated with lymphoid metastasis and Dukes grade (P < 0.05), and survivin-?Ex3 was associated with invasiveness. We concluded that mRNA expression rates and levels of survivin and its four splice variants elevated in CRC tissues, and expression levels of survivin-?Ex3 and survivin-3B were positively associated with tumor aggression.
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Probe design rules and effective enzymes for endonuclease-based detection of nucleic acids.
Bioorg. Med. Chem.
PUBLISHED: 02-10-2013
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Junction probe (JP) platform is an isothermal endonuclease-based detection assay for both RNA and DNA. Herein, we screen 31 REAse and identify effective restriction endonucleases that can be used for JP detection. Secondly, we investigate how different probe architectures affect JP cleavage rates and conclude that although molecular beacon (MB) JP probes give less background noise than linear JP probes, the cleavage of MB JP probes are slower than linear JP probes.
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An on-bacterium flow cytometric immunoassay for protein quantification.
J Pharm Biomed Anal
PUBLISHED: 02-08-2013
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The polystyrene bead-based flow cytometric immunoassay has been widely reported. However, the preparation of functional polystyrene bead is still inconvenient. This study describes a simple and easy on-bacterium flow cytometric immunoassay for protein quantification, in which Staphylococcus aureus (SAC) is used as an antibody-antigen carrier to replace the polystyrene bead. The SAC beads were prepared by carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling, paraformaldehyde fixation and antibody binding. Carcinoembryonic antigen (CEA) and cytokeratin-19 fragment (CYFRA 21-1) proteins were used as models in the test system. Using prepared SAC beads, biotinylated proteins, and streptavidin-phycoerythrin (SA-PE), the on-bacterium flow cytometric immunoassay was validated by quantifying CEA and CYFRA 21-1 in sample. Obtained data demonstrated a concordant result between the logarithm of the protein concentration and the logarithm of the PE mean fluorescence intensity (MFI). The limit of detection (LOD) in this immunoassay was at least 0.25 ng/ml. Precision and accuracy assessments appeared that either the relative standard deviation (R.S.D.) or the relative error (R.E.) was <10%. The comparison between this immunoassay and a polystyrene bead-based flow cytometric immunoassay showed a correlation coefficient of 0.998 for serum CEA or 0.996 for serum CYFRA 21-1. In conclusion, the on-bacterium flow cytometric immunoassay may be of use in the quantification of serum protein.
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Somatostatin derivative (smsDX) targets cellular metabolism in prostate cancer cells after androgen deprivation therapy.
PLoS ONE
PUBLISHED: 02-07-2013
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Cancer cell metabolism responsive to androgen deprivation therapy (ADT) may be involved in the development and progression of prostate cancer and the ultimate failure of androgen-deprivation therapy. To investigate the metabolism regulation effects on androgen-independent growth of prostate cancer, an established LNCaP-s cell model that resembles the clinical scenario of castration-resistant prostate cancer (CRPC), was used in this current study. This cell line was cultured from androgen-sensitive LNCaP parental cells, in an androgen-reduced condition, resembling clinical androgen deprivation therapy. To assess the effects of smsDX on the invasiveness of prostate cancer cells we used wound healing assay and Matrigel™ invasion assay. We evaluated differentially expressed proteins of the parental LNCaP cells and LNCaP-s cells after ADT by means of two-dimensional gel electrophoresis (2-DE) followed by MALDI-TOF mass spectrometric analysis. The covered area in the wound and the number of cells invading through a Matrigel chamber were significantly smaller for cells treated with smsDX than they were for control cells treated with vehicle. 56 proteins were found differentially expressed in LNCaP-s cells compared to LNCaP cells, majority of them were down-regulated after ADT treatment. 104 proteins of LNCaP cells and 86 in LNCaP-s cells, separately, were found differentially expressed after treatment with smsDX, When we explored these protein functions within the website UniProtKB/Swiss-Prot, surprisingly, most of the proteins were found to be involved in the cellular metabolism and mitochondrial function regulation. LNCaP-s as potential metastatic androgen-independent cancer cells, its metabolism and mitochondrial functions could be altered by a new somatostatin derivative smsDX, the smsDX regulatory effects on metabolism in LNCaP-s deliver more therapeutic information with the treatment of CRPC.
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Dual-specificity phosphatase 6 (Dusp6), a negative regulator of FGF2/ERK1/2 signaling, enhances 17?-estrodial-induced cell growth in endometrial adenocarcinoma cell.
Mol. Cell. Endocrinol.
PUBLISHED: 02-06-2013
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Dual-specificity phosphatase 6 (Dusp6) is a negative feedback mechanism of fibroblast growth factors (FGFs)/mitogen-activated protein kinase (MAPK)/ERK1/2 signaling. The aim of this study was to explore the expression of Dusp6 in human endometrial adenocarcinomas and the role of Dusp6 expression in the growth regulation of endometrial adenocarcinoma cell. We found that Dusp6 was over-expressed in human endometrial adenocarcinomas. In Ishikawa cells, plasmid-driven Dusp6 expression efficiently blocked the activity of FGF2-induced MAPK/ERK1/2 signaling. Unexpectedly, Dusp6 expression significantly enhanced the growth of Ishikawa cells. In Dusp6 forced-expression cells, 17?-estradiol stimulation increased the cell growth by all most threefolds. In addition, progesterone treatment reduced the cell growth to about half both in Ishikawa cells with and without forced-Dusp6-expression. Dusp6 over-expression is involved in the pathogenesis and development of human endometrial adenocarcinomas. Dusp6 functions as a negative regulator of FGF2/ERK1/2 signaling but enhances the growth and 17?-estradiol-induced cell growth in endometrial adenocarcinoma cell.
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The effects of qindan-capsule-containing serum on the TGF-?1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts.
Pharm Biol
PUBLISHED: 02-04-2013
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Qindan capsule (QC), a compound used in traditional Chinese medicine, has been used as an anti-hypertensive agent in clinical settings for years. Our previous studies have shown that QC can improve the morphological index of the artery, down-regulate the collagen volume fraction in the media and inhibit the transformation of smooth muscle cells. However, the detailed mechanisms underlying its effects require further investigation, which might provide more scientific evidence for the clinical treatment of hypertensive vascular remodeling (VR).
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A novel lignin degradation bacterial consortium for efficient pulping.
Bioresour. Technol.
PUBLISHED: 01-23-2013
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A lignin degradation bacterial consortium named LDC was screened from the sludge of a reeds pond by a restricted subculture. It could break down 60.9% lignin in reeds at 30°C under conditions of static culture within 15 days. In order to analyze the diversity of LDC, plate isolation, 16S rDNA clone library and ARDRA (Amplified Ribosomal DNA Restriction Analysis) were performed. Six bacterial strains were isolated from LDC and eighteen DNA phylotypes were identified from 230 bacterial analyzed clones. They were classified into Clostridiales(9.1%), Geovibrio thiophilus (5.1%), Desulfomicrobium (10.9%), Pseudomonas sp. (25.2%), Azoarcus sp. (5.1%), Thauera (5.1%), Paenibacillus sp. (5.1%), Cohnella sp. (2.2%), Acinetobacter sp. (3.1%), Microbacterium (7.8%), and uncultured bacterium (21.3%). In addition, physical characteristics of paper hand-sheets between biological pretreatment and chemical pretreatment were compared. The results showed that LDC had the capability of lignin degradation and was efficient for pulping, which would provide a new choice for biopulping.
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Fluorouracil selectively enriches stem-like cells in the lung adenocarcinoma cell line SPC.
Tumour Biol.
PUBLISHED: 01-17-2013
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Most adult stem cells are in the G0 or quiescent phase of the cell cycle and account for only a small percentage of the cells in the tissue. Thus, isolation of stem cells from tissues for further study represents a major challenge. This study sought to enrich cancer stem cells and explore cancer stem-like cell clones using 5-fluorouracil (5-FU) in the lung adenocarcinoma cell line, SPC. Proliferation inhibition was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, according to which half maximal inhibitory concentration values were calculated. Expression levels of stem cell markers after treatment with 5-FU were examined using immunofluorescence and Western blotting. Additionally, side population (SP) cells were sorted using FACS. Properties of SP cells were evaluated by using Transwell, colony-forming assays, and tumor formation experiments. 5-FU greatly inhibits proliferation, especially of cells in S phase. SP cells possess greater invasive potential, higher clone-forming potential, and greater tumor-forming ability than non-SP cells. Treatment with 5-FU enriches the SP cells with stem cell properties in human lung adenocarcinoma cell lines.
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Disruption of the intestinal mucosal barrier in Candida albicans infections.
Microbiol. Res.
PUBLISHED: 01-15-2013
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Candida albicans is a common microorganism in the intestine. However, invasive C. albicans infection has emerged as a life-threatening disease in recent years. The mortality rate of invasive candidiasis is high in critically ill hosts. C. albicans can switch from the yeast to the hyphal morphology, and take advantage of the impaired intestinal mucosal barrier and insufficient immunity of the host to facilitate its colonization and penetration. Despite the availability of potent new antifungal drugs in recent years, the treatment of severe candidiasis, especially candidaemia, has not been substantially improved. In this review, the virulence factors of C. albicans, as well as the antagonistic role of the intestinal mucosal barrier will be discussed to illuminate the mechanisms of C. albicans enterogenic infections.
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Hepatitis B reactivation in chronic myeloid leukemia patients receiving tyrosine kinase inhibitor.
World J. Gastroenterol.
PUBLISHED: 01-09-2013
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Hepatitis B virus (HBV) reactivation is a well-recognized complication in patients with chronic HBV infection receiving cytotoxic or immunosuppressive chemotherapy. Imatinib mesylate and nilotinib are selective Bcr/Abl tyrosine kinase inhibitors, which are now widely used in the treatment of patients with chronic myeloid leukemia. Although HBV reactivation induced by imatinib mesylate has been reported, nilotinib-related HBV reactivation has not been reported in the English literature. We report here 2 cases of HBV reactivation in chronic myeloid leukemia patients receiving imatinib mesylate and a novel case of nilotinib related HBV reactivation.
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Effects of common polymorphisms rs2910164 in miR-146a and rs3746444 in miR-499 on cancer susceptibility: a meta-analysis.
Mol. Biol. Rep.
PUBLISHED: 01-06-2013
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MicroRNAs (miRNAs) are a class of new non-coding RNA, which may play a more important role in the pathogenesis of human cancers. Rs2910164 in miR-146a and rs3746444 in miR-499 are shown to be associated with increased/decreased cancer risk. We performed a meta-analysis to systematically summarize the possible association. We retrieved the relevant articles from PubMed databases. Studies were selected using specific inclusion and exclusion criteria. ORs and 95% CIs were calculated to access the strength of association between microRNA polymorphism and cancer risk. All analyses were performed using the Stata software. Twenty-nine studies were included in this meta-analysis. There were not significant associations between miR-146a rs2910164 and miR-499 rs3746444 polymorphisms with overall cancer risk. In the subgroup analysis by ethnicity, significantly affected cancer risks were found among Asians for both rs2910164 (GC vs. GG: OR = 0.89, 95% CI = 0.82-0.96; CC vs. GG: OR = 0.80, 95% CI = 0.66-0.97; GC + CC vs. GG: OR = 0.86, 95% CI = 0.76-0.97; C vs. G: OR = 0.91, 95% CI = 0.82-1.00) and rs3746444 (GG + AG vs. AA: OR = 1.21, 95% CI = 1.00-1.46). In the tumor type subgroup analysis, rs2910164 C allele decreased the risk of hepatocellular carcinoma (C vs. G: OR = 0.89, 95% CI = 0.80-1.00) and cervical squamous cell carcinoma (C vs. G: OR = 0.72, 95% CI = 0.62-0.84). The rs2910164 in miR-146a and the rs3746444 in miR-499 are likely to be associated with cancer risk.
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Quercetin inhibits left ventricular hypertrophy in spontaneously hypertensive rats and inhibits angiotensin II-induced H9C2 cells hypertrophy by enhancing PPAR-? expression and suppressing AP-1 activity.
PLoS ONE
PUBLISHED: 01-01-2013
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Quercetin is the most abundant flavonoid in fruit and vegetables and is believed to attenuate cardiovascular disease. We hypothesized that quercetin inhibits cardiac hypertrophy by blocking AP-1 (c-fos, c-jun) and activating PPAR-? signaling pathways.
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Compositional mining of multiple object API protocols through state abstraction.
ScientificWorldJournal
PUBLISHED: 01-01-2013
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API protocols specify correct sequences of method invocations. Despite their usefulness, API protocols are often unavailable in practice because writing them is cumbersome and error prone. Multiple object API protocols are more expressive than single object API protocols. However, the huge number of objects of typical object-oriented programs poses a major challenge to the automatic mining of multiple object API protocols: besides maintaining scalability, it is important to capture various object interactions. Current approaches utilize various heuristics to focus on small sets of methods. In this paper, we present a general, scalable, multiple object API protocols mining approach that can capture all object interactions. Our approach uses abstract field values to label object states during the mining process. We first mine single object typestates as finite state automata whose transitions are annotated with states of interacting objects before and after the execution of the corresponding method and then construct multiple object API protocols by composing these annotated single object typestates. We implement our approach for Java and evaluate it through a series of experiments.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.