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Find video protocols related to scientific articles indexed in Pubmed.
Urban and rural factors associated with life satisfaction among older Chinese adults.
Aging Ment Health
PUBLISHED: 11-20-2014
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Objective: This study compared urban and rural factors associated with life satisfaction among older adults in mainland China. Method: Study data were extracted at random from 10% of the Sample Survey on Aged Population in urban/rural China in 2006 for 1980 participants aged 60 and older, including 997 from urban cities and 983 from rural villages. Results: In this study, 54.6% of urban older adults and 44.1% of rural older adults reported satisfaction with their lives. Binary logistic regression analysis showed that financial strain, depressive symptoms, filial piety, and accessibility of health services were significantly associated with life satisfaction for both urban and rural participants, but age and financial exchange with children were only associated with life satisfaction among urban older adults. Conclusion: Findings are consistent with some previous studies that indicated the importance of financial strain, depressive symptoms, filial piety, and accessibility of health services to life satisfaction among the older adults in both urban and rural areas. This study also demonstrated the importance of age and family financial exchange to the life satisfaction of urban older adults.
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Refractive index dependent real-time plasmonic nanoprobes on a single silver nanocube for ultrasensitive detection of the lung cancer-associated miRNAs.
Chem. Commun. (Camb.)
PUBLISHED: 11-20-2014
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We developed a novel method for the real-time monitoring of the delicate change in refractive index (RI) when DNA or RNA hybridize near a DNA-capped silver nanocube (AgNC) surface. This method offers an alternative platform in the quantitative analysis of the trace lung cancer-associated miRNAs in label-free detection.
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Smart pH-responsive upconversion nanoparticles for enhanced tumor cellular internalization and near-infrared light-triggered photodynamic therapy.
Chem. Commun. (Camb.)
PUBLISHED: 11-20-2014
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A smart pH-responsive photodynamic therapy system based on upconversion nanoparticle loaded PEG coated polymeric lipid vesicles (RB-UPPLVs) was designed and prepared. These RB-UPPLVs which are promising agents for deep cancer photodynamic therapy applications can achieve enhanced tumor cellular internalization and near-infrared light-triggered photodynamic therapy.
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OX40- and CD27-Mediated Costimulation Synergizes with Anti-PD-L1 Blockade by Forcing Exhausted CD8+ T Cells To Exit Quiescence.
J. Immunol.
PUBLISHED: 11-19-2014
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Exhaustion of chronically stimulated CD8(+) T cells is a significant obstacle to immune control of chronic infections or tumors. Although coinhibitory checkpoint blockade with anti-programmed death ligand 1 (PD-L1) Ab can restore functions to exhausted T cell populations, recovery is often incomplete and dependent upon the pool size of a quiescent T-bet(high) subset that expresses lower levels of PD-1. In a model in which unhelped, HY-specific CD8(+) T cells gradually lose function following transfer to male bone marrow transplantation recipients, we have explored the effect of shifting the balance away from coinhibition and toward costimulation by combining anti-PD-L1 with agonistic Abs to the TNFR superfamily members, OX40 and CD27. Several weeks following T cell transfer, both agonistic Abs, but especially anti-CD27, demonstrated synergy with anti-PD-L1 by enhancing CD8(+) T cell proliferation and effector cytokine generation. Anti-CD27 and anti-PD-L1 synergized by downregulating the expression of multiple quiescence-related genes concomitant with a reduced frequency of T-bet(high) cells within the exhausted population. However, in the presence of persistent Ag, the CD8(+) T cell response was not sustained and the overall size of the effector cytokine-producing pool eventually contracted to levels below that of controls. Thus, CD27-mediated costimulation can synergize with coinhibitory checkpoint blockade to switch off molecular programs for quiescence in exhausted T cell populations, but at the expense of losing precursor cells required to maintain a response.
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[Phenotype and functions of natural killer cells in septic patients and its clinical significance].
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
PUBLISHED: 11-18-2014
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To investigate the possible mechanism of natural killer cells (NK cells) in immune dysfunction in sepsis by monitoring the phenotype and function of periphery NK cells in patients with sepsis.
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In Situ Generation of Electron Acceptor for Photoelectrochemical Biosensing via Hemin-Mediated Catalytic Reaction.
Anal. Chem.
PUBLISHED: 11-14-2014
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A novel photoelectrochemical sensing strategy is designed for DNA detection on the basis of in situ generation of electron acceptor via the catalytic reaction of hemin toward H2O2. The photoelectrochemical platform was established by sequential assembly of near-infrared CdTe quantum dots, capture DNA and hemin-labeled DNA probe to form triple-helix molecular beacon (THMB) structure on indium tin oxide electrode. According to highly catalytic capacity of hemin towards H2O2, a photoelectrochemical mechanism was then proposed, in which the electron acceptor of O2 was in situ generated on the electrode surface, leading to the enhancement of photocurrent response. The utilization of CdTe QDs can extend the absorption edge to near-infrared band, resulting in the increase of light-to-electricity efficiency. After introducing target DNA, the THMB structure is disassembled and releases hemin, and thus quenches the photocurrent. Under optimized conditions, this biosensor shows high sensitivity with the linear range from 1 to 1000 pM and detection limit of 0.8 pM. Moreover, it exhibits good performance of excellent selectivity, high stability and acceptable fabrication reproducibility. This present strategy opens an alternative avenue for photoelectrochemical signal transduction, and expands the applications of hemin-based materials in photoelectrochemical biosensing and clinical diagnosis.
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One-Pot Synthesis of multi-Substituted 2-Aminoquinolines from Annulation of 1-Aryl Tetrazoles with Internal Alkynes via Double C-H Activation and Denitrogenation.
J. Org. Chem.
PUBLISHED: 11-13-2014
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An efficient and one-pot synthesis of multi-substituted 2-aminoquinolines from 1-aryl tetrazoles and internal alkynes has been developed. The reaction involves cyclization of 1-aryl tetrazoles with internal alkynes via rhodium(III)-catalyzed double C-H activation, and copper(II)-mediated denitrogenation.
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Rhizobacter bergeniae sp. nov., isolated from the root of Bergenia scopulosa.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 11-13-2014
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A yellowish-pigmented bacterium, designated strain PLGR-1T, was isolated from the root of Bergenia scopulosa collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. Cells of strain PLGR-1T were Gram-negative, strictly aerobic, rod-shaped, non-spore-forming and motile with a single polar flagellum. Growth occurred at 7-33 °C (optimum, 25-28 °C), at pH 5.0-10.0 (optimum, pH 6.0-7.0) and with 0-0.5 % (w/v) NaCl (optimum, 0 %). The predominant respiratory quinone was ubiquinone-8 (Q-8) and the major cellular fatty acids were summed feature 3 (comprising C16:1?7c and/or C16:1?6c), C16:0 and summed feature 8 (comprising C18:1?7c and/or C18:1?6c). The major polyamines are putrescine and 2-hydroxyputrescine and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 69.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain PLGR-1T belonged to the class Betaproteobacteria and formed a tight phyletic lineage with members of the genus Rhizobacter. Strain PLGR-1T was most closely related to Rhizobacter dauci DSM 11587T and Rhizobacter fulvus DSM 19916T, with 16S rRNA gene sequence similarities of 98.5 % and 98.0 %, respectively. The DNA-DNA relatedness values between strain PLGR-1T and the type strains of R. dauci and R. fulvus were 46.3 and 14.7 %, respectively. Based on the phenotypic, phylogenetic and genotypic data, strain PLGR-1T is considered to represent a novel species of the genus Rhizobacter, for which the name Rhizobacter bergeniae sp. nov. is proposed. The type strain is PLGR-1T (= CCTCC AB 2013018T = KCTC 32299T = LMG 27607T).
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Microbial community in anoxic-oxic-settling-anaerobic sludge reduction process revealed by 454 pyrosequencing analysis.
Can. J. Microbiol.
PUBLISHED: 11-13-2014
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Modification of the anoxic-oxic (AO) process by inserting a sludge holding tank (SHT) into the sludge return line forms an anoxic-oxic-settling-anaerobic (A+OSA) process that can achieve a 48.98% sludge reduction rate. The 454 pyrosequencing method was used to obtain the microbial communities of the AO and A+OSA processes. Results showed that the microbial community structures of the 2 processes were different as a result of the SHT insertion. Bacteria assigned to the phyla Proteobacteria and Bacteroidetes commonly existed and dominated the microbial populations of the 2 processes. However, the relative abundance of these populations shifted in the presence of SHT. The relative abundance of Proteobacteria decreased during the A+OSA process. A specific comparison at the class level showed that Sphingobacteria was enriched in the A+OSA process. The result suggested that the fermentative bacteria Sphingobacteria may have key functions in reducing the sludge from the A+OSA process. Uncultured Nitrosomonadaceae gradually became the dominant ammonia-oxidizing bacteria, and the nitrite-oxidizing bacterium Nitrospira was enriched in the A+OSA process. Both occurrences were favorable for stabilized nitrogen removal. The known denitrifying species in the A+OSA process were similar to those in the AO process; however, their relative abundance also decreased.
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Histone deacetylase inhibitors for enhancing activity of antifungal agent: a patent evaluation of WO2014041424(A1).
Expert Opin Ther Pat
PUBLISHED: 11-09-2014
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Novel histone deacetylase inhibitors have been developed for the antifungal therapy. Molecule 8 exhibited potent antifungal activities with MIC values of 0.25/0.25, 0.12/0.25, 0.12/0.12 µg/ml against Candida albicans, C. parapsilosis and C. glabrata after 24/48 h incubation, respectively. Most of the synthesized compound showed significantly synergistic effects with fluconazole in the biological assay. The discovery of these molecules makes positive contributions to the development of potent and safe antifungal drugs.
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Detection and Biological Activities of Carboxyethylpyrrole Ethanolamine Phospholipids: a Correlation with Age-related Macular Degeneration.
Chem. Res. Toxicol.
PUBLISHED: 11-08-2014
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Oxidation of docosahexaenoate phospholipids produces 4-hydroxy-7-oxo-hept-5-eonyl phospholipids (HOHA)-PLs that react with protein lysyl ?-amino residues to generate 2-?-carboxyethylpyrrole (CEP) derivatives, endogenous factors that induce angiogenesis in the retina and tumors. It seemed likely but remained unproven that HOHA-PLs react with ethanolamine phospholipids (EPs) in vivo to generate CEP-EPs. We now show that CEP-EPs are present in human blood at 4.6-fold higher levels in age-related macular degeneration plasma than in normal plasma. We also show that a CEP-EPs are pro-angiogenic, inducing tube formation by human umbilical vein endothelial cells by activating the Toll-like receptor 2. CEP-EP levels may be a useful biomarker for clinical assessment of AMD risk and CEP-associated tumor progression and a tool for monitoring the efficacy of therapeutic interventions.
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Discordance of HIV and HSV-2 biomarkers and self-reported sexual behaviour among orphan adolescents in Western Kenya.
Sex Transm Infect
PUBLISHED: 11-08-2014
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This paper examines the discordance between biological data of HIV and herpes simplex virus type 2 (HSV-2) infections and self-reported questionnaire responses among orphan adolescents in Western Kenya.
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Biofunctionalization of Si(111)7×7 by "Renewable" l-Cysteine Transitional Layer.
J. Am. Chem. Soc.
PUBLISHED: 11-07-2014
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Surface functionalization of an inorganic surface with bio-organic molecules is often aimed at creating a "permanent" bio-organic surface with receptor functional groups. We show here that l-cysteine can be used to transform a highly reactive Si(111)7×7 surface to not just a permanent bio-organic surface but also a semipermanent (or renewable) and a temporary bio-organic surfaces by manipulating the exposure. In the early growth stage, the strong bonding between the first cysteine adlayer and the Si substrate through Si-N or Si-S linkages in unidentate or bidentate arrangement provides permanent biofunctionalization by this interfacial layer. This interfacial layer can be used to build a transitional layer (second adlayer) mediated by interlayer vertical hydrogen bonding between an amino group and a carboxylic acid group. Further exposure of cysteine eventually leads to a zwitterionic multilayer film involving electrostatic interactions between cation (-NH3(+)) and anion moieties (-COO(-)). The interlayer hydrogen bonding therefore provides temporary trapping of bio-organic molecules as the second transitional layer that is stable up to 175 °C. This transitional layer can be easily removed by annealing above this temperature and then regenerated with the same molecular layer or a different one by "renewing" the interlayer hydrogen bonds. We also illustrate coverage-dependent adsorption structures of cysteine, from bidentate to unidentate attachments and to self-assembled multimers, involving formation of intralayer horizontal N···H-O hydrogen bonds, by combining our X-ray photoemission data with the local density-of-state images obtained by scanning tunnelling microscopy.
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Demonstration of a 3-bit optical digital-to-analog converter based on silicon microring resonators.
Opt Lett
PUBLISHED: 11-01-2014
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We propose an N-bit optical digital-to-analog converter based on silicon microring resonators (MRRs), which can transform an N-bit electrical digital signal to an optical analog signal. A 3-bit optical digital-to-analog convertor is fabricated as proof of concept through a CMOS-compatible process on a silicon-on-insulator platform. The silicon MRRs are modulated through the electric-field-induced carrier injection in forward biased PN junctions embedded in the ring waveguides. The electro-optical 3-dB bandwidths of the silicon MRRs are approximately 800 MHz. The device works well at a speed of 500??MSample/s under driving voltage swings of 0.75 V.
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The recycling of incinerated sewage sludge ash (ISSA) as a raw material for CaO-Al2O3-SiO2-P2O5 glass-ceramic production.
Environ Technol
PUBLISHED: 11-01-2014
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Abstract In this paper, the recycling of incinerated sewage sludge ash (ISSA) in glass-ceramic materials by a two-stage sintering cycle of nucleation stage and crystallization stage without any pressure and binder are presented. The procedure which parent glasses were subjected to each nucleation/crystallization temperature and time level was the following: A, 790°C, 1.0?h/870°C, 1.0?h-3.0?h; B, 790°C, 1.0?h/945°C, 1.0?h-3.0?h; and C, 790°C, 1.0?h/1065°C, 1.0?h-3.0?h. XRD analysis results revealed that multiple crystalline phases coexisted in the glass-ceramic materials and the crystalline phases compositions were more affected by crystallization temperature than crystallization time. SEM analysis showed an interlocking microstructure of glass phases and crystals with different sizes and spatial distribution. The glass-ceramics crystallized at 945°C for 2.0?h exhibited optimal properties of density of 2.88±0.08?g/cm(3), compression strength of 247±12?MPa, bending strength of 118±14?MPa and water absorption of 0.42±0.04. The leaching concentrations of heavy metals were far lower than the limits required by the regulatory standard of EPA. This paper provided a feasible, low cost and promising method to produce ISSA-based glass-ceramics and highlighted the principal characteristics that must be taken into account to use incinerated sewage sludge ash correctly in glass-ceramics.
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[The research on residual amplitude modulation characteristics in fiber frequency modulation spectroscopy].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 11-01-2014
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Frequency modulation spectroscopy (FMS) not only can be used to simultaneously measure the absorption and dispersion of atoms and molecules, but is the key technology of the noise immunity cavity enhanced optical heterodyne molecular spectroscopy (NICE-OHMS). The optical devices or the instability of output light polarization of the laser source will induce the residual amplitude modulation (RAM) in the FMS. RAM greatly limits the FMS technology application in trace gas detection, so the research on the RAM characteristics in the FMS has very important significance. Firstly, the lineshape of FMS without absorption was analyzed, and the impact factors on the RAM were acquired, then the influence of input and output polarization direction and electro-optical modulation (EOM) temperature was measured, respectively. They all have linear relationship with the RAM. The results verify the theoretical analysis and provide the basis for reducing the RAM and other related working.
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Thoracic Epithelioid Malignant Vascular Tumors: A Clinicopathologic Study of 52 Cases With Emphasis on Pathologic Grading and Molecular Studies of WWTR1-CAMTA1 Fusions.
Am. J. Surg. Pathol.
PUBLISHED: 10-30-2014
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Malignant thoracic epithelioid vascular tumors are an uncommon and heterogenous group of tumors that include low-grade to intermediate-grade epithelioid hemangioendothelioma (EHE) and high-grade epithelioid angiosarcoma (EAS). We examine the morphologic and immunohistochemical features of 52 malignant epithelioid vascular tumors (10 low-grade EHE, 29 intermediate-grade EHE, and 13 EAS) involving the thorax (lung, pleura, mediastinum, heart, great vessels) including cases with exclusively thoracic disease (35) and with multiorgan disease including the thorax (17). Intermediate-grade EHE differs from low-grade EHE by the presence of necrosis, increased mitotic activity, and increased atypia. Morphologic features such as intranuclear inclusions, intracytoplasmic vacuoles, and stromal changes (chondroid, myxoid, or hyalinized stroma) are seen more frequently in EHE, whereas blood lakes, proliferation of slit-like vessels, and prominent nucleoli favor EAS. Fluorescence in situ hybridization analysis showed CAMTA1-WWTR1 fusions in 4/7 low-grade and 23/23 intermediate-grade EHE (P<0.001). In EAS, CAMTA1 rearrangement was negative in all cases, whereas a WWTR1 complex abnormality was found in 1/5 cases (P<0.001). This offers an objective means of differentiating intermediate-grade EHE from EAS, especially on limited biopsies. All cases show expression of at least 1 vascular marker, which allows differentiation from primary thoracic epithelial malignancies, although keratin expression is a potential pitfall with 29% of EHE and 25% of EAS showing keratin expression. Survival analysis shows that higher tumor grade for all tumors (P=0.026) as well as lung and pleural tumors only (P=0.010) and the presence of pleural involvement in lung and/or pleural tumors (P=0.042) correlate with poor prognosis.
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Primary Renal Sclerosing Epithelioid Fibrosarcoma: Report of 2 Cases With EWSR1-CREB3L1 Gene Fusion.
Am. J. Surg. Pathol.
PUBLISHED: 10-30-2014
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We report the first 2 genetically confirmed cases of primary renal sclerosing epithelioid fibrosarcoma (SEF), occurring in a 17-year-old boy and a 61-year-old woman. In both cases, the tumors demonstrated the typical epithelioid clear cell morphology associated with extensive hyalinizing fibrosis, raising the differential diagnosis of solitary fibrous tumor, metanephric stromal tumor, and the sclerosing variant of clear cell sarcoma of the kidney. Both neoplasms demonstrated diffuse immunoreactivity for MUC4, a highly specific marker for SEF, and both demonstrated evidence of rearrangement of both the EWSR1 and CREB3L1 genes, which have recently been shown to be fused in this entity. Both neoplasms presented with metastatic disease. Primary renal SEF represents yet another translocation-associated sarcoma now shown to arise primarily in the kidney.
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Calsyntenin3: Molecular Architecture and Interaction with Neurexin 1alpha.
J. Biol. Chem.
PUBLISHED: 10-30-2014
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Calsyntenin 3 (Cstn3 or Clstn3), a recently identified synaptic organizer, promotes the development of synapses. Cstn3 localizes to the postsynaptic membrane and triggers presynaptic differentiation. Calsyntenin members play an evolutionarily conserved role in memory and learning. Cstn3 was recently shown in cell-based assays to interact with neurexin 1alpha (n1?), a synaptic organizer that is implicated in neuropsychiatric disease. Interaction would permit Cstn3 and n1? to form a trans-synaptic complex and promote synaptic differentiation. However, it is contentious whether Cstn3 binds n1? directly. To understand the structure and function of Cstn3 we determined its architecture by electron microscopy and delineated the interaction between Cstn3 and n1? biochemically and biophysically. We show that Cstn3 ectodomains form monomers as well as tetramers that are stabilized by disulfide bonds and Ca(2+), and both are likely flexible in solution. We show further that the extracellular domains of Cstn3 and n1? interact directly and that both Cstn3 monomers and tetramers bind n1? with nanomolar affinity. The interaction is promoted by Ca2+ and requires minimally the LNS domain of Cstn3. Furthermore, Cstn3 uses a fundamentally different mechanism to bind n1? compared to other neurexin partners such as the synaptic organizer neuroligin 2 (NL2), because Cstn3 does not strictly require the sixth LNS domain of n1?. Our structural data suggest how Cstn3 as a synaptic organizer on the postsynaptic membrane, particularly in tetrameric form, may assemble radially symmetric trans-synaptic bridges with the presynaptic synaptic organizer n1? to recruit and spatially organize proteins into networks essential for synaptic function.
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A Kernel Classification Framework for Metric Learning.
IEEE Trans Neural Netw Learn Syst
PUBLISHED: 10-28-2014
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Learning a distance metric from the given training samples plays a crucial role in many machine learning tasks, and various models and optimization algorithms have been proposed in the past decade. In this paper, we generalize several state-of-the-art metric learning methods, such as large margin nearest neighbor (LMNN) and information theoretic metric learning (ITML), into a kernel classification framework. First, doublets and triplets are constructed from the training samples, and a family of degree-2 polynomial kernel functions is proposed for pairs of doublets or triplets. Then, a kernel classification framework is established to generalize many popular metric learning methods such as LMNN and ITML. The proposed framework can also suggest new metric learning methods, which can be efficiently implemented, interestingly, using the standard support vector machine (SVM) solvers. Two novel metric learning methods, namely, doublet-SVM and triplet-SVM, are then developed under the proposed framework. Experimental results show that doublet-SVM and triplet-SVM achieve competitive classification accuracies with state-of-the-art metric learning methods but with significantly less training time.
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[Expression of follistatin, activin A and BMP-4 in rats with hypoxic-ischemic brain damage].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 10-25-2014
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To investigate the expression and significance of follistatin, activin A and bone morphogenetic protein-4 (BMP-4) in normal brain tissues of rats and the brain tissues with hypoxic and ischemicgin.
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Ultrasound utility for predicting biological behavior of invasive ductal breast cancers.
Asian Pac. J. Cancer Prev.
PUBLISHED: 10-24-2014
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The aim of the study was to evaluate the correlation of ultrasound features with breast cancer molecular status.
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[Influencing factors for operational performance of a biofilm reactor with microbubble aeration using SPG membrane].
Huan Jing Ke Xue
PUBLISHED: 10-24-2014
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The microbubble-aerated biofilm reactor provides a feasibility to apply microbubble aeration in aerobic wastewater treatment processes. In this study, Shirasu porous glass (SPG) membranes were used for microbubble aeration in a fixed bed biofilm reactor treating synthetic municipal wastewater. The influencing factors for operational performance of the bioreactor were investigated, including operating parameters, SPG membrane fouling and its structural changes. The results indicated that there was no significant influences of air flux, organic loading rate and packed bed on COD removal and an average COD removal efficiency of 80% -90% could be achieved under different operating conditions. On the other hand, the dissolved oxygen (DO) concentrations decreased significantly along with reducing air flux or increasing organic loading rate. As a result, the ammonia removal deteriorated gradually and the average ammonia removal efficiency decreased from 80% -90% to 20% -30% At the same time, the total nitrogen (TN) removal achieved in the simultaneous nitrification and denitrification process was also reduced from 30% -40% to about 20% , due to nitrification inhibition. Higher available porosity could be obtained when ring packing was used in the fixed bed, resulting in improvement of contaminant removal performance. An oxygen utilization efficiency of close to 100% could be achieved at low air fluxes or high organic loading rates during microbubble aeration. Both biofilm growth and organic foulant accumulation on SPC, membrane surface contributed to membrane fouling after long-term operation. The average pore size and porosity of SPG membrane increased significantly due to the chemical corrosion caused by alkali NaClO solution used for online cleaning. Then the air permeation of SPG membrane was affected by membrane fouling and destroyed pore structure.
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Metal-Ligand Binding Interactions in Rhodium/Palladium-Catalyzed Synthesis of Dihydroquinolines.
J. Org. Chem.
PUBLISHED: 10-23-2014
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A domino Rh- and Pd-catalyzed synthesis of dihydroquinolines is disclosed. Two metals and two ligands are placed in one reaction vessel along with the two reactive reagents to afford selective sequential coupling despite the potential for side reactions. In this report, we describe mechanistic investigations attempting to discern the catalyst-ligand interactions occurring in this domino reaction. Through these studies, the reactivity and relative catalyst ligand loadings were successfully tuned to efficiently access the heterocyclic products.
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Microvessel density as a prognostic factor in bladder cancer: A systematic review of literature and meta-analysis.
Cancer Biomark
PUBLISHED: 10-23-2014
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Many studies have shown that microvessel density (MVD) could play a potential value as prognostic biomarkers of tumors. The aim of this systematic review and meta-analysis is to evaluate the association between MVD and survival in patients with bladder cancer.
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[The comparison of the micro-syringe with clinical usage].
Zhongguo Yi Liao Qi Xie Za Zhi
PUBLISHED: 10-22-2014
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Micro-syringe as a result of the high frequency of clinical use, direct contact with patients, the failure of its equipment will have a direct impact on the patient's disease status. Researchers in this paper, by simulating the test fluid, electrical safety testing and analysis of statistical methods such as maintenance records, consider six kinds of common injection pump, in terms of performance, safety and the failure rate of three performance. Compared the performance analysis, summarized the various types of injection pump characteristics, researchers make suggestions to clinical departments, equipment procurement and equipment management departments for usage and improvement.
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Cobalt-catalyzed enantioselective hydroboration of 1,1-disubstituted aryl alkenes.
J. Am. Chem. Soc.
PUBLISHED: 10-21-2014
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We report the synthesis of cobalt complexes of novel iminopyridine-oxazoline (IPO) ligands and their application to the asymmetric hydroboration of 1,1-disubstituted aryl alkenes. The new catalysts afforded ?-alkyl-?-pinacolatoboranes with exclusive regioselectivity in high yields with up to 99.5% ee. Furthermore, we have applied this method to an efficient synthesis of naproxen.
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[The synergistic effect of amygdalin and HSYA on the IL-1beta induced endplate chondrocytes of rat intervertebral discs].
Yao Xue Xue Bao
PUBLISHED: 10-18-2014
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The effect of amygdalin joint hydroxysafflor yellow A (HSYA) on the endplate chondrocytes derived from intervertebral discs of rats induced by IL-1beta and the possible mechanism were studied and explored. Chondrocytes were obtained from endplate of one-month SD rat intervertebral discs and cultured primary endplate chondrocytes. After identification, they were divided into normal group, induced group, amygdalin group, HSYA group and combined group. CCK-8 kit was adopted to detect the proliferation of the endplate chondrocytes. FCM was measured to detect the apoptosis. Real-time PCR method was adopted to observe the mRNA expression of Aggrecan, Col 2 alpha1, Col 10 alpha1, MMP-13 and the inflammatory cytokines IL-1beta. The protein expression of Col II, Col X was tested through immunofluorescence. Compared with the normal group, the proliferation of the endplate chondrocytes decreased while the apoptosis increased (P < 0.05). With down regulation of the mRNA expressions of Aggrecan, Col 2 alpha1 and up regulation of the mRNA expressions of Col 10 alpha1, MMP-13, IL-1beta (P < 0.05), the protein expression of Col II decreased while the protein expression of Col X increased. Compared with the induced group, amygdalin group, HSYA group, the combined group could inhibit the apoptosis and promote the proliferation (P < 0.05). They could increase the mRNA expressions of Aggrecan and Col 2 alpha1 while decrease the mRNA expressions of Col 10 alpha1, MMP-13 and IL-1beta (P < 0.05). They could also enhance the protein expression of Col II while reduce the protein expression of Col X. The effect of the combined group was significantly better than that of amygdalin and HSYA. Amygdalin joint HSYA could inhibit the degeneration of the endplate chondrocytes derived from intervertebral discs of rats induced by IL-1beta and better than the single use of amygdalin or HSYA.
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Experimentally excellent beaming in a two-layer dielectric structure.
Opt Express
PUBLISHED: 10-17-2014
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We demonstrate both experimentally and theoretically that a two-layer dielectric structure can provide collimation and enhanced transmission of a Gaussian beam passing through it. This is due to formation of surface localized states along the layered structure and the coupling of these states to outgoing propagating waves. A system of multiple cascading two-layers can sustain the beaming for large propagation distances.
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Non-null annular subaperture stitching interferometry for steep aspheric measurement.
Appl Opt
PUBLISHED: 10-17-2014
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A non-null annular subaperture stitching interferometry (NASSI), combining the subaperture stitching ideal and non-null test method, is proposed for steep aspheric testing. Compared with standard annular subaperture stitching interferometry (ASSI), a partial null lens (PNL) is employed as an alternative to the transmission sphere, to generate different aspherical wavefronts as the references. The coverage subaperture number would be reduced greatly for the better performance of aspherical wavefronts in matching the local slope of aspheric surfaces. In this way, relatively large overlapping areas can be obtained for adjustment errors correction while the error accumulation would be decreased. With the reverse optimization reconstruction (ROR) method for retrace error correction, the figure error of each subaperture can be retrieved accurately. Therefore, the testing accuracy and efficiency are thus increased. The dynamic test range is extended as well. A numerical simulation exhibits the comparison of the performance of the NASSI and standard ASSI, which demonstrates the high accuracy of the NASSI in testing steep aspheric. Experimental results of NASSI are shown to be in good agreement with those of the Zygo interferometer.
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Reverse optimization reconstruction of aspheric figure error in a non-null interferometer.
Appl Opt
PUBLISHED: 10-17-2014
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Aspheric non-null testing, as an alternative to the traditional null testing, achieves more flexible measurements. However, figure-error reconstruction in non-null tests has always been difficult due to the presence of retrace error. A novel method with reverse optimization is proposed for aspheric figure-error reconstruction in a non-null interferometer. It is a generalized and effective approach based on system modeling and polynomial fitting. An optimization function is set with polynomial coefficients of the desired figure error as variables and those of the detected experimental wavefront as optimization targets. Through the reverse optimization process with iterative ray tracing, the optimal solutions can be extracted and the desired figure error is reconstructed with a simple fitting procedure. Numerical simulations verifying the high accuracy of the proposed method are presented with error considerations. A set of experiments has also been carried out to demonstrate the validity and repeatability of this method.
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Drosophila CK2 promotes Wts to suppress Yki activity for growth control.
J. Biol. Chem.
PUBLISHED: 10-17-2014
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Drosophila Hippo signaling regulates Wts activity to phosphorylate and inhibit Yki in order to control tissue growth. CK2 is widely expressed and involved in a variety of signaling pathways. In this study we report that Drosophila CK2 promotes Wts activity to phosphorylate and inhibit Yki activity, which is independent of Hpo induced Wts promotion. In vivo, CK2 overexpression suppresses hpo mutant induced Ex upregulation and overgrowth phenotype while it cannot affect wts mutant. Consistent with this, knockdown of CK2 upregulates Hpo pathway target expression. We also found that Drosophila CK2 is essential for tissue growth as a cell death inhibitor as knockdown of CK2 in developing disc induces severe growth defect as well as caspase3 signal. Taken together, our results uncover a dual role of CK2: while its major role is promoting cell survive, it may potentially be a growth inhibitor as well.
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Targeting the c-Met/FZD8 signaling axis eliminates patient-derived cancer stem-like cells in head and neck squamous carcinomas.
Cancer Res.
PUBLISHED: 10-17-2014
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Cancer stem-like cells (CSC) thought to contribute to head and neck squamous carcinomas (HNSCC) may offer attractive therapeutic targets if a tractable approach can be developed. In this study, we report that silencing c-Met is sufficient to suppress sphere formation, tumor initiation and metastatic properties of HN-CSC. Pharmacologic inhibition of c-Met with the selective inhibitor PF-2341066 preferentially targeted CSC and synergized with conventional chemotherapy to improve efficacy in a mouse xenograft model of HNSCC, impeding tumor growth and reducing metastasis. Mechanistic investigations showed that CSC elimination was due to downregulation of Wnt/?-catenin signaling in HN-CSC and that the Wnt pathway receptor FZD8 was essential for interactions of c-Met and Wnt/?-catenin signaling in HN-CSC. Notably, ectopic expression of FZD8 rescued the impaired phenotype of HN-CSC where c-Met was inhibited. Furthermore, c-Met upregulated FZD8 through the ERK/c-Fos cascade in HN-CSC. Taken together, our results offer a preclinical proof-of-concept for targeting the c-Met/FZD8 signaling axis as a CSC-directed therapy to improve HNSCC treatment.
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S100A12 expression in patients with primary biliary cirrhosis.
Immunol. Invest.
PUBLISHED: 10-15-2014
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Objectives: S100 calcium binding protein A12 (S100A12) has been supposed to be a pro-inflammatory factor associated with non-infectious inflammatory diseases. However, whether S100A12 is involved in the inflammatory process of primary biliary cirrhosis (PBC) has not been shown. Methods: The levels of S100A12 mRNA transcripts in peripheral mononuclear blood cells (PBMCs) of 66 Chinese patients with primary biliary cirrhosis (PBC), 62 healthy controls (HC) and 55 chronic hepatitis B (CHB) were measured by qRT-PCR. S100A12 serum concentrations in 34 PBC patients were measured by ELISA. Results: The levels of S100A12 mRNA transcripts in PBMCs of patients with PBC were significantly higher than healthy controls (p?
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Hepatic MAP kinase phosphatase-1 Selectively Regulates Glucose Metabolism and Energy Homeostasis.
Mol. Cell. Biol.
PUBLISHED: 10-15-2014
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The liver plays a critical role in glucose metabolism and communicates with peripheral tissues to maintain energy homeostasis. Obesity and insulin resistance are highly associated with non-alcoholic fatty liver disease (NAFLD). However, the precise molecular details of NAFLD remain incomplete. The p38 mitogen-activated protein kinase (MAPK) and c-Jun NH2-terminal kinase (JNK) regulate liver metabolism. However, the physiological contribution of MAPK phosphatase-1 (MKP-1) as a nuclear antagonist of both p38 MAPK and JNK in liver is unknown. Here we show that hepatic MKP-1 becomes overexpressed following high fat feeding. Liver-specific deletion of MKP-1 enhances gluconeogenesis and causes hepatic insulin-resistance in chow-fed mice, whilst selectively conferring protection from hepatosteatosis upon high fat feeding. Further, hepatic MKP-1 regulates both interleukin-6 (IL-6) and fibroblast growth factor 21 (FGF21). Mice lacking hepatic MKP-1 exhibit reduced circulating IL-6 and FGF21 levels that were associated with impaired skeletal muscle mitochondrial oxidation and susceptibility to diet-induced obesity. Hence, hepatic MKP-1 serves as a selective regulator of MAPK-dependent signals that contributes to the maintenance of glucose homeostasis and peripheral tissue energy balance. These results also demonstrate that hepatic MKP-1 overexpression in obesity as causally linked to the promotion of hepatosteatosis.
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[Genome sequencing and the molecular evolutionary analysis of a SFTSV isolated from Zhejiang province].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 10-15-2014
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To sequence the whole genome and to analyze the molecular and evolutionary of Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) isolated from Zhejiang province.
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The Noninvasive Detection of RAR?2 Promoter Methylation for the Diagnosis of Prostate Cancer.
Cell Biochem. Biophys.
PUBLISHED: 10-14-2014
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Prostate cancer is a kind of commonly diagnosed male malignancy. With the aging population in China, both incidence and mortality of prostate cancer are expected to keep increasing in the future. The methylation of RAR?2 gene promoter is a common molecular event in prostate cancer. Thus, we aimed at establishing a high-performance noninvasive DNA methylation assay based on pyrosequencing for screening of prostate cancer in this article. The assay is designed to detect aberrant promoter methylation of RAR?2 gene in ejaculate samples. The negative and positive control plasmids were constructed with different treatments by direct bisulfite conversion or conversion after Sss I Methylase methylation to establish quality control standard. The ejaculate and tissue samples were collected from patients with histologically confirmed adenocarcinoma of prostate (n = 43) and benign prostatic hyperplasia (n = 40). Significant correlation was observed between prostate cancer and methylation level of RAR?2 gene promoter. In addition, the results of pyrosequencing in ejaculate samples were compared with that of DNA sequencing in tissue samples from the same patients. There is no significant difference in the detection of RAR?2 promotor methylation between these two methods (p < 0.05). In conclusion, we have developed a high-performance noninvasive DNA methylation assay based on pyrosequencing which is more suitable for high-throughput detection of aberrant promoter methylation in ejaculate samples. Moreover, the acceptive degree of this noninvasive method makes it potentially promising for future screening of prostate cancer.
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[Clinical application of the disposable circumcision suture device in male circumcision].
Zhonghua Nan Ke Xue
PUBLISHED: 10-14-2014
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To investigate the safety and efficiency of the disposable circumcision suture device (DCSD) in the surgical treatment of phimosis and redundant prepuce.
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Molecular spintronics: destructive quantum interference controlled by a gate.
J. Am. Chem. Soc.
PUBLISHED: 10-10-2014
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The ability to control the spin-transport properties of a molecule bridging conducting electrodes is of paramount importance to molecular spintronics. Quantum interference can play an important role in allowing or forbidding electrons from passing through a system. In this work, the spin-transport properties of a polyacetylene chain bridging zigzag graphene nanoribbons (ZGNRs) are studied with nonequilibrium Green's function calculations performed within the density functional theory framework (NEGF-DFT). ZGNR electrodes have inherent spin polarization along their edges, which causes a splitting between the properties of spin-up and spin-down electrons in these systems. Upon adding an imidazole donor group and a pyridine acceptor group to the polyacetylene chain, this causes destructive interference features in the electron transmission spectrum. Particularly, the donor group causes a large antiresonance dip in transmission at the Fermi energy EF of the electrodes. The application of a gate is investigated and found to provide control over the energy position of this feature making it possible to turn this phenomenon on and off. The current-voltage (I-V) characteristics of this system are also calculated, showing near ohmic scaling for spin-up but negative differential resistance (NDR) for spin-down.
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[Weight change in association with the incidence of type 2 diabetes in adults from Qingdao, China].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 10-09-2014
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Since the incidence rates and risk factor for type 2 diabetes in Chinese populations had not been well known, the aim of this study was to evaluate the impact of weight change and other risk factors on incident type 2 diabetes in Qingdao, China.
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Electric control of spin in monolayer WSe2 field effect transistors.
Nanotechnology
PUBLISHED: 10-07-2014
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We report first-principles theoretical investigations of quantum transport in a monolayer WSe2 field effect transistor (FET). Due to strong spin-orbit interaction (SOI) and the atomic structure of the two-dimensional lattice, monolayer WSe2 has an electronic structure that exhibits Zeeman-like up-down spin texture near the K and [Formula: see text] points of the Brillouin zone. In a FET, the gate electric field induces an extra, externally tunable SOI that re-orients the spins into a Rashba-like texture thereby realizing electric control of the spin. The conductance of FET is modulated by the spin texture, namely by if the spin orientation of the carrier after the gated channel region, matches or miss-matches that of the FET drain electrode. The carrier current [Formula: see text] in the FET is labelled by both the valley index and spin index, realizing valleytronics and spintronics in the same device.
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Aryl Bromides as Inexpensive Starting Materials in the Catalytic Enantioselective Arylation of Aryl Aldehydes: The Additive TMEDA Enhances the Enantioselectivity.
J. Org. Chem.
PUBLISHED: 10-04-2014
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We used aryl bromides as inexpensive starting materials to enantioselectively arylate aldehydes in one pot. Aryl bromides readily transfer aryls to aryllithiums with n-butyllithium, successively to triarylaluminums with aluminum chloride, and then to aryltitaniums with titanium isopropoxide. Finally aryltitaniums arylate aldehydes catalyzed by (S)-H8-BINOL-Ti(Oi-Pr)2 in excellent yields and enantioselectivities. The additive TMEDA evidently suppresses the racemic background reaction promoted by LiCl generated from salt metathesis. This procedure represents a cost-effective and operationally convenient method for enantioenriched diarylmethanols.
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Crossover Formation During Rice Meiosis Relies on Interaction of OsMSH4 and OsMSH5.
Genetics
PUBLISHED: 10-04-2014
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MSH4 encodes a MutS protein that plays a specialized role in meiosis. In eukaryotic species, such as budding yeast, mice, Caenorhabditis elegans and Arabidopsis, msh4 mutants display meiotic defects with a reduced number of chiasmata. Here, we characterized rice MSH4 by map-based cloning. In Osmsh4 mutants, the chiasma frequency was dramatically decreased to approximately 10% of the wild type, but the synaptonemal complex was normally installed. The double mutant analysis showed that in the Osmsh4 Osmsh5 mutant, the reduction of chiasmata was greater than other zmm mutants. This was consistent with the absence of localization for OsZIP4 and OsMER3 in Osmsh4, and suggests an earlier role for OsMSH4 and OsMSH5 than other ZMM proteins where they may be required to stabilize progenitor Holliday junctions. Using yeast two-hybrid and pull-down assays, we verified the direct physical association between OsMSH4 and OsMSH5, OsMSH5 and HEI10 in plants for the first time. The MSH4-MSH5 heterodimer has been demonstrated in mammals to stabilize the formation of progenitor and double Holliday junctions (dHjs) that may be resolved as crossovers. We propose that OsMSH4 interacts with OsMSH5 to promote formation of the majority of crossovers in rice.
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Continuous and scalable production of well-controlled noble-metal nanocrystals in milliliter-sized droplet reactors.
Nano Lett.
PUBLISHED: 10-03-2014
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Noble-metal nanocrystals are essential to applications in a variety of areas, including catalysis, electronics, and photonics. Despite the large number of reports, there still exists a gap between academic studies and industrial applications due to the lack of ability to produce the nanocrystals in large quantities while still maintaining the good uniformity and precise controls. Because the nucleation and growth of colloidal nanocrystals are highly sensitive to experimental conditions, it is impractical to scale up their production by simply increasing the reaction volume. Here we report a new and practical approach based on milliliter-sized droplet reactors to the scalable production of nanocrystals. The droplets of 0.25 mL in volume were produced as a continuous flow in a fluidic device assembled from commercially available components. As a proof of concept, we have synthesized Pd, Au, and Pd-M (M = Au, Pt, and Ag) nanocrystals with controlled sizes, shapes, compositions, and structures on a scale of 1-10 g per hour (e.g., 3.6 g per hour for Pd cubes of 10 nm in edge length).
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[Simultaneous determination of zeranols and chloramphenicol in foodstuffs of animal origin by combination immunoaffinity column clean-up and liquid chromatography-tandem mass spectrometry].
Se Pu
PUBLISHED: 10-02-2014
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A combination immunoaffinity column (IAC-CZ) clean-up and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical method was successfully developed for zearalenol, beta-zearalenol and zearalenone) and chloramphenicol (CAP) in foodstuffs of animal origin. The samples (fish, liver, milk and honey) were enzymatically digested by beta-glucuronidase/sulfatase for about 16 h and then extracted with ether. The extracts were evaporated to dryness and then the residues were dissolved by 1.0 mL of 50% acetonitrile solution. After filtered and diluted with PBS buffer, the reconstituted solution were cleaned-up with a IAC-CZ and then analyzed by LC-MS/MS in multiple reaction monitoring (MRM) mode. The chromatographic separation was performed on a Shimadzu Shim-pack VP-ODS column with gradient elution by acetonitrile and 2 mmol/L ammonium acetate solution. The detection was carried out by electrospray negative ionization mass spectrometry in MRM mode. The proposed method was validated by the limit of detection (0.04-0.10 microg/kg), linearity (R2 > or = 0.999 0), average recoveries (70.9%-95.6%) and precisions (2.0% - 11.8%). The developed method is reliable, sensitive and has good applicability. The combination immunoaffinity column was proved to be an effective pretreatment technique to decrease the matrix effect, and it met the requirements of residue analysis of co-occurring zeranols and chloramphenicol.
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Current situation of transvaginal mesh repair for pelvic organ prolapse.
Chin. Med. Sci. J.
PUBLISHED: 09-30-2014
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Surgical mesh is a metallic or polymeric screen intended to be implanted to reinforce soft tissue or bone where weakness exists. Surgical mesh has been used since the 1950s to repair abdominal hernias. In the 1970s, gynecologists began using surgical mesh products to indicate the repair of pelvic organ prolapse (POP), and in the 1990s, gynecologists began using surgical mesh for POP. Then the U.S. Food and Drug Administration (FDA) approved the first surgical mesh product specifically for use in POP. Surgical mesh materials can be divided into several categories. Most surgical mesh devices cleared for POP procedures are composed of non-absorbable synthetic polypropylene. Mesh can be placed in the anterior vaginal wall to aid in the correction of cystocele (anterior repair), in the posterior vaginal wall to aid in correction of rectocele (posterior repair), or attached to the top of the vagina to correct uterine prolapse or vaginal apical prolapse (apical repair). Over the past decades, surgical mesh products for transvaginal POP repair became incorporated into "kits" that included tools to aid in the delivery and insertion of the mesh. Surgical mesh kits continue to evolve, adding new insertion tools, tissue fixation anchors, surgical techniques, and ab- sorbable and biological materials. This procedure has been performed popularly. It was also performed increased in China. But this new technique met some trouble recently and let shake in urogynecology.
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[¹H NMR metabonomics study of pancreatic extracts from insulin-resistant rats induced by fructose feeding].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 09-30-2014
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To study the metabolic changes of pancreatic extracts from insulin-resistant rats induced by fructose feeding using nuclear magnetic resonance ¹H spectroscopy (¹H NMR).
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Aberrant CD200/CD200R1 expression and its potential role in Th17 cell differentiation, chemotaxis and osteoclastogenesis in rheumatoid arthritis.
Rheumatology (Oxford)
PUBLISHED: 09-28-2014
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CD200/CD200R1 signalling has an immunoregulatory effect on the activation threshold of the inflammatory immune response and maintains immune homeostasis. In this study we evaluated the status of CD200/CD200R1 interaction in patients with RA.
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Predicting the Effect of Cytochrome P450 Inhibitors on Substrate Drugs: Analysis of Physiologically Based Pharmacokinetic Modeling Submissions to the US Food and Drug Administration.
Clin Pharmacokinet
PUBLISHED: 09-28-2014
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The US Food and Drug Administration (FDA) has seen a recent increase in the application of physiologically based pharmacokinetic (PBPK) modeling towards assessing the potential of drug-drug interactions (DDI) in clinically relevant scenarios. To continue our assessment of such approaches, we evaluated the predictive performance of PBPK modeling in predicting cytochrome P450 (CYP)-mediated DDI.
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[Simultaneous determination of 16 organophosphorous pesticides in vegetables, fruits and tea by gas chromatography coupled with clean-up by mesoporous alumina as solid-phase extraction adsorbent].
Se Pu
PUBLISHED: 09-27-2014
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A gas chromatographic method based on solid-phase extraction was developed for the simultaneous determination of 16 organophosphorous pesticides in vegetables, fruits and tea, including cabbage, lettuce, pumpkin, onion, tomato, turnip, apple, pear and tea. The samples were extracted with ethyl acetate, and clean-up with mesoporous alumina as solid-phase extraction adsorbent. The separation of target compounds was performed on a DB-1701 capillary column, and the quantitative analysis of the organophosphorous pesticides was carried out by gas chromatography with flame photometric detection. The results showed that the calibration curves of the 16 organophosphorous pesticides were linear in the range of 10-2 000 microg/L with good correlation coefficients (R2 > 0.997). The recoveries of the pesticides in different samples at three spiked levels ranged from 83.2% to 103.8% with the relative standard deviations of 2.0%-9.9%. This method has high sensitivity, high accuracy and good repeatability, and can be applied to the determination of the organophosphorus pesticide residues in vegetables, fruits and tea.
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R-spondin1 is a novel hormone mediator for mammary stem cell self-renewal.
Genes Dev.
PUBLISHED: 09-26-2014
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Signals from the niche play pivotal roles in regulating adult stem cell self-renewal. Previous studies indicated that the steroid hormones can expand mammary stem cells (MaSCs) in vivo. However, the facilitating local niche factors that directly contribute to the MaSC expansion remain unclear. Here we identify R-spondin1 (Rspo1) as a novel hormonal mediator in the mammary gland. Pregnancy and hormonal treatment up-regulate Rspo1 expression. Rspo1 cooperates with another hormonal mediator, Wnt4, to promote MaSC self-renewal through Wnt/?-catenin signaling. Knockdown of Rspo1 and Wnt4 simultaneously abolishes the stem cell reconstitution ability. In culture, hormonal treatment that stimulates the expression of both Rspo1 and Wnt4 can completely substitute for exogenous Wnt proteins, potently expand MaSCs, and maintain their full development potential in transplantation. Our data unveil the intriguing concept that hormones induce a collaborative local niche environment for stem cells.
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[Evaluation of left ventricular myocardial function in coronary atherosclerosis patients with type 2 diabetes mellitus by speckle tracking echocardiography].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 09-26-2014
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To evaluate the left ventricular (LV) myocardial function in coronary atherosclerosis patients with type 2 diabetes mellitus (DM) by two-dimensional speckle tracking echocardiography (2D STE).
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[Chronic disseminated intravascular coagulopathy associated with aortic dissecting aneurysms: two cases report and literature review].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 09-24-2014
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To report two cases of chronic disseminated intravascular coagulation associated (DIC) with aortic dissecting aneurysm, and discuss the treatment strategy.
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[Clinical characteristics and risk factors for major thrombosis in 604 Chinese patients with low-risk essential thrombocythemia].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 09-24-2014
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To analyze clinical and molecular characteristics of low-risk essential thrombocythemia (ET) in a large cohort of Chinese patients and to explore risk factors for major thrombosis and treatment strategies.
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[A randomized controlled trial: acclimatization training on the prevention of motion sickness in hot-humid environment].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
PUBLISHED: 09-24-2014
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Incidence and severity of motion sickness (MS) in hot-humid environment are extremely high. We tried to know the effect of two-stage training for reducing incidence and severity of ms.
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Palladium-catalyzed difunctionalization of alkynes via C-N and S-N cleavages: a versatile approach to highly functional indoles.
Org. Lett.
PUBLISHED: 09-23-2014
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Palladium-catalyzed intramolecular addition of C-N and S-N bond to alkynes with the migration of functional groups has been achieved. A wide range of functional groups including acyl, pyruvoyl, amide, and sulfonyl groups can migrate smoothly and be conveniently introduced at the C-3 postion of indoles in our catalytic system. The operational simplicity and broad substrate scope demonstrate the great potential of this method for the synthesis of highly functional indoles.
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[Manipulative reduction with minimally invasive percutaneous plate osteosynthesis for 60 patients with distal tibiofibular fractures].
Zhongguo Gu Shang
PUBLISHED: 09-23-2014
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To explore clinical effects of manipulative reduction with minimally invasive percutaneous plate osteosynthesis in treating distal tibiofibular fractures.
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pH-dependent assembly of two polyoxometalate host-guest structural isomers based on Keggin polyoxoanion templates.
Dalton Trans
PUBLISHED: 09-18-2014
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Two interesting polyoxometalate-templated host-guest compounds and with the same chemical formulae of [Ag6(tpt)4(SiW12O40)Cl2]·2H2O have been obtained by hydrothermal reactions of monolacunary Keggin-type K8[?-SiW11O39]·13H2O with CH3COOAg and tpt ligands under different pH conditions. Compounds and are structural isomers. In , the novel chair-like tetranuclear [Ag4(?3-Cl)2](2+) clusters and additional Ag(+) ions are linked by tpt ligands to form a 2D double-layered structure featured with two different types of voids in the layer, and the double-layers are stacked into a 3D host supramolecular framework with 1D channels filled with Keggin [SiW12O40](4-) polyoxoanions and water molecules as guests. In , Ag(+) ions are linked by tpt ligands into a 3D framework of 10(3)-ths topology. The most outstanding structural feature of is that upon six-fold interpenetration among 10(3)-ths nets there are still nanosized channels along the a axis to accommodate Keggin polyoxoanions and water molecules as guests. Compound represents the first example of six-fold interpenetrating cationic networks templated with polyoxoanions. In this work, the obvious differences in structure between and show that hydrochloric acid solution conditions, which offer not only H(+) to regulate the pH value but also Cl(-) to coordinate with Ag(+) ions, play an important role in the formation of host frameworks templated by Keggin-type polyoxoanions. The photoluminescence properties of and have been investigated in the solid state.
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Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins.
J. Gen. Virol.
PUBLISHED: 09-18-2014
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The interferon (IFN) immune system plays an essential role in protecting the host against most viral infections. In order to explore the interactions between the IFN pathway and Respiratory syncytial virus (RSV) infection, and to identify potential IFN-stimulated genes (ISGs) that may be involved in suppressing the replication of RSV, we utilized an IFN pathway-specific microarray to study the effects of RSV infection on the IFN pathway in HeLa cells. We showed that RSV infection enhanced the expression of a series of ISGs, including oligoadenylate synthetase 2 (OAS2), interferon-induced transmembrane protein 1 (IFITM1) and myxovirus-resistance 2 (Mx2). Our results also showed that the IFITM proteins potently inhibited RSV infection mainly by interfering with both virus entry and the subsequent replication steps, but not the attachment process. The anti-viral effect of IFITM3 protein was not affected by ubiquitination modification. Furthermore, knocking down the endogenous and IFN-induced expressions of IFITM1 and IFITM3 proteins facilitated RSV infection. Expression of the IFITM proteins was found to delay the phosphorylation of interferon regulatory factor-3 (IRF3) through interfering with the detection of viral RNA by the melanoma differentiation-associated gene 5 (MDA5) and the retinoic acid-inducible gene I (RIG-I). These results demonstrated that the restriction of RSV infection by the IFITM proteins was achieved through the inhibition of virus entry and replication, and they provide further insight for exploring the mechanism of IFITM proteins-mediated virus restriction.
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Multi-boundary cardiac data visualization based on multidimensional transfer function with ray distance.
Biomed Mater Eng
PUBLISHED: 09-18-2014
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A crucial role during the implementation of volume visualization is to identify the optimal transfer function, since the vital information and structure can be highlighted and revealed. The boundary of the volume is shared by respective portion of the two materials formed out of it, which causes undesirable thickening and ambiguity of the boundary explored via traditional LH (Low and High) histogram. To address this issue, initially a modified LH histogram construction method is introduced to intuitively and conveniently visualize cardiac volume for user interaction. Subsequently, the f-LH histogram is presented to further identify and visualize each portion of the boundary accurately. An appropriate multidimensional transfer function generation is proposed by using variables in f-LH space and spatial information, for visualizing the multi-boundary cardiac volume data.
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[Clinical literature based statistical analysis of common Chinese medical syndrome types].
Zhongguo Zhong Xi Yi Jie He Za Zhi
PUBLISHED: 09-17-2014
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According to the principle of evidence-based medicine (EBM), Chinese medical literatures based descriptive statistical analysis of common Chinese medical syndrome types were performed. By data extraction, standardization, and frequency calculation of disease names and syndrome types from 286 literatures in line with the inclusion criteria, the frequencies of diseases and syndromes were obtained to analyze common syndrome types in clinical practice, to analyze the distribution features of disease related syndromes and syndrome related diseases, to analyze the distribution of basic Chinese medical syndrome types in clinical common diseases as a whole, thus providing reference for clinical and basic researches.
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A Pilot Study of Galectin-3, HBME-1, and p27 Triple Immunostaining Pattern for Diagnosis of Indeterminate Thyroid Nodules in Cytology With Correlation to Histology.
Appl. Immunohistochem. Mol. Morphol.
PUBLISHED: 09-16-2014
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Indeterminate thyroid nodules form a heterogenous group of lesions that constitute 5% to 30% of thyroid cytology diagnoses. We introduce a triple immunostaining protocol for subtyping. Galectin-3, HBME-1, and p27 triple immunostaining, performed on destained cytology slides and formalin-fixed paraffin-embedded tissue, was developed and applied to 51 patients retrospectively with preoperative cytologic diagnoses of follicular lesion of undetermined significance (n=40), atypia of undetermined significance (n=6), and suspicious for follicular neoplasm (n=5). The malignant rate in this series was 43.1% (22/51). A hierarchal evaluation algorithm was generated based on digital image quantitation of triple-stained histologic sections, and applied to both cytology and histology specimens. Fifty of 51 cytology cases have triple staining validated by internal controls. In cytology specimens, the individual sensitivities and specificities of p27, Galectin3, and HBME1 for cancer with 95% confidence interval are: 86.2% (0.674, 0.955)/66.7% (0.431, 0.845); 77.3% (0.542, 0.913)/72.4% (0.525, 0.866); and 72.7% (0.496, 0.884)/93.1% (0.758, 0.988), respectively. Sensitivity is increased to 95.5% (0.751, 0.998), but specificity is decreased to 69.0% (0.490, 0.840), if Galectin3 and HBME1 are both used in combination as markers for malignancy. However, the level of specificity is increased to 86.2% (0.674, 0.955) and sensitivity remains high 100% (0.808, 1) if in addition, using the Galectin3/HBME1:p27 ratio (ratio ?2 indicating malignancy) for 2 or 3 markers positive cases. Thus, the triple staining method on cytology slides and histology sections shows a similar sensitivity/specificity/positive predictive value/negative predictive value of 100.0%/86.2%/84.0%/100.0% and 95.5%/86.2%/84.0%/96.2%, respectively (P=0.92). Overall, p27 is the most frequent single positive marker (19/50, 38% in cytology), consistent with benign nature of most indeterminate thyroid nodules. Galectin-3 and HBME-1 colocalization (positive in the same cell) was demonstrated in thyroid cancer in 45.5% (10/22) of histology sections, but in none of the normal thyroid tissues and benign thyroid lesions. This supports the notion that synchronous activation of Galectin-3 and HBME-1 occurs in thyroid malignancy and is highly specific for malignancy. We have demonstrated the performance and pattern of triple immunostaining for subtyping indeterminate thyroid nodules. Further studies and validation in different larger populations are warranted.This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivitives 3.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially. http://creativecommons.org/licenses/by-nc-nd/3.0.
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[Laparoscopic hepatectomy for recurrent hepatocellular carcinoma after previous open hepatectomy].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 09-16-2014
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To evaluate the feasibility, safety and long-term outcomes of laparoscopic repeat hepatectomy for recurrent hepatocellular carcinoma (HCC) following previous resection.
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Controlled integration of oligo- and polythiophenes at the molecular scale.
Phys Chem Chem Phys
PUBLISHED: 09-13-2014
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High molecular weight PBTTT-C12 is blended with the pure trimer, BTTT-3, to enhance intergrain connectivity and charge transport. Analysis of the morphology and crystallinity of the blends shows that the polymer and oligomer are well-integrated, leading to high hole mobilities, greater than 0.1 cm(2) V(-1) s(-1), in films that contain as much as 83% oligomer.
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Genomic evolution of Saccharomyces cerevisiae under Chinese rice wine fermentation.
Genome Biol Evol
PUBLISHED: 09-13-2014
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Rice wine fermentation represents a unique environment for the evolution of the budding yeast, Saccharomyces cerevisiae. To understand how the selection pressure shaped the yeast genome and gene regulation, we determined the genome sequence and transcriptome of a S. cerevisiae strain YHJ7 isolated from Chinese rice wine (Huangjiu), a popular traditional alcoholic beverage in China. By comparing the genome of YHJ7 to the lab strain S288c, a Japanese sake strain K7, and a Chinese industrial bioethanol strain YJSH1, we identified many genomic sequence and structural variations in YHJ7, which are mainly located in subtelomeric regions, suggesting that these regions play an important role in genomic evolution between strains. In addition, our comparative transcriptome analysis between YHJ7 and S288c revealed a set of differentially expressed genes, including those involved in glucose transport (e.g., HXT2, HXT7) and oxidoredutase activity (e.g., AAD10, ADH7). Interestingly, many of these genomic and transcriptional variations are directly or indirectly associated with the adaptation of YHJ7 strain to its specific niches. Our molecular evolution analysis suggested that Japanese sake strains (K7/UC5) were derived from Chinese rice wine strains (YHJ7) at least approximately 2,300 years ago, providing the first molecular evidence elucidating the origin of Japanese sake strains. Our results depict interesting insights regarding the evolution of yeast during rice wine fermentation, and provided a valuable resource for genetic engineering to improve industrial wine-making strains.
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7.0T nuclear magnetic resonance evaluation of the amyloid beta (1-40) animal model of Alzheimer's disease: comparison of cytology verification.
Neural Regen Res
PUBLISHED: 09-11-2014
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3.0T magnetic resonance spectroscopic imaging is a commonly used method in the research of brain function in Alzheimer's disease. However, the role of 7.0T high-field magnetic resonance spectroscopic imaging in brain function of Alzheimer's disease remains unclear. In this study, 7.0T magnetic resonance spectroscopy showed that in the hippocampus of Alzheimer's disease rats, the N-acetylaspartate wave crest was reduced, and the creatine and choline wave crest was elevated. This finding was further supported by hematoxylin-eosin staining, which showed a loss of hippocampal neurons and more glial cells. Moreover, electron microscopy showed neuronal shrinkage and mitochondrial rupture, and scanning electron microscopy revealed small size hippocampal synaptic vesicles, incomplete synaptic structure, and reduced number. Overall, the results revealed that 7.0T high-field nuclear magnetic resonance spectroscopy detected the lesions and functional changes in hippocampal neurons of Alzheimer's disease rats in vivo, allowing the possibility for assessing the success rate and grading of the amyloid beta (1-40) animal model of Alzheimer's disease.
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Asticcacaulis endophyticus sp. nov., a prosthecate bacterium isolated from the root of Geum aleppicum.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 09-11-2014
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A strictly aerobic, light-yellow-coloured, stalked bacterium, designated strain ZFGT-14T, was isolated from the root of Geum aleppicum Jacq. collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. This novel isolate grew at 7-33 °C (optimum, 25-28 °C) and pH 6.0-10.0 (optimum, pH 7.0-8.0). Flexirubin-type pigments were not produced. Cells were Gram-reaction-negative, rod-shaped and motile with a single polar flagellum. The predominant respiratory quinone was Q-10. The major cellular fatty acids were summed feature 8 (comprising C18:1?7c/C18:1?6c), C16:0, C19:0 cyclo ?8c and summed feature 3 (comprises C16:1?7c and/or C16:1?6c) and the major polar lipids were phosphatidylglycerol and glycolipids. The DNA G+C content was 57.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZFGT-14T was most closely related to the genus Asticcacaulis, exhibiting low sequence similarity (95.0-95.9 %) with all Asticcacaulis species with validly published names. Based on the phenotypic, phylogenetic and genotypic data, strain ZFGT-14T is considered to represent a novel species of the genus Asticcacaulis, for which the name Asticcacaulis endophyticus sp. nov. is proposed. The type strain is ZFGT-14T (=CCTCC AB 2013012T = KCTC 32296T=LMG 27605T).
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