The protective effects of Acanthus Ilicifolius alkaloid A and its derivatives on pro- and anti-inflammatory cytokines in rats with hepatic fibrosis.
This study was designed to investigate the protective effects of Ilicifolius Alkaloid A (4-hydroxy-2-benzoxazolone, HBOA) and its acetylated derivatives including 4-acetoxy-2-benzoxazolone (TC-2) and 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3), on CCl4 -induced liver fibrosis in rats. Sprague-Dawley rats were given CCl4 twice per week for 8 weeks to induce liver fibrosis. Then they were treated with HBOA, TC-2 and TC-3 daily for 4 weeks, respectively. The serum indicators including total protein, albumin, globulin, hyaluronic acid and laminin were measured by commercial kits. The mRNA expression of adiponectin, peroxisome proliferator-activated receptor-gamma (PPAR-?), tumor necrosis factor-? (TNF-?), interleukin-6 (IL-6), transforming growth factor-?1 (TGF- ?1 ) and toll like receptor 4 (TLR4 ) were determined by RT-PCR. The proteins of adiponectin, TGF-?1 , ?-smooth muscle actin (?-SMA) and TLR4 were also detected by immunohistochemical assay. The results showed that HBOA, TC-2 and TC-3 significantly attenuated the fibrotic degree induced by CCl4 as evidenced by higher levels of total protein, albumin, adiponectin and PPAR-? which in turn decreased the proliferation of hepatic stellate cells. Moreover, those drugs markedly decreased the levels of hyaluronic acid, laminin, TNF-?, IL-6, TGF-?1 , ?-SMA and TLR4 . Our study indicates that HBOA, TC-2 and TC-3 have beneficial effects against liver fibrosis, and the mechanisms may be related to the inhibition of inflammatory response. This article is protected by copyright. All rights reserved.