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Find video protocols related to scientific articles indexed in Pubmed.
High-Pitch Coronary CT Angiography at 70 kVp With Low Contrast Medium Volume: Comparison of 80 and 100 kVp High-Pitch Protocols.
Medicine (Baltimore)
PUBLISHED: 11-15-2014
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The purpose of this article is to evaluate image quality and radiation dose of prospectively electrocardiogram (ECG)-triggered high-pitch coronary computed tomography angiography (CCTA) at 70?kVp and 30?mL contrast medium.One hundred fifty patients with a heart rate ?70 beats per minute (bpm) underwent CCTA using a second-generation dual-source computed tomography (CT) scanner and were randomized into 3 groups according to tube voltage and contrast medium volume (370?mg/mL iodine concentration) (100 kVp group, 100 kVp/60?mL, n?=?55; 80 kVp group, 80 kVp/60?mL, n?=?44; 70 kVp group, 70 kVp/30?mL, n?=?51). Objective and subjective image quality along with the effect of heart rate (HR) and body mass index (BMI) was evaluated and compared between the groups. Radiation dose was estimated for each patient.CT attenuation and image noise were higher in the 80 and 70 kVp groups than in the 100 kVp group (all P??0.05). HR did not affect subjective image quality (all P?>?0.05), while patients with BMI <23?kg/m had higher image quality than patients with BMI ?23?kg/m (P?
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Reduced tumorigenicity and drug resistance through the downregulation of octamer-binding protein 4 and Nanog transcriptional factor expression in human breast stem cells.
Mol Med Rep
PUBLISHED: 10-20-2014
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Breast cancer is the most common type of malignancy among females. Previous studies examining breast cancer tissue have demonstrated the presence of stem cells, and have detected octamer?binding protein 4 (Oct4) and Nanog transcription factor expression. In the present study, breast cancer stem cells (CSCs) were isolated and enriched from MDA?MB?231 breast cancer cell lines, and were defined as MDA?MB?231 stem cells using flow cytometry. The expression of Oct4 and Nanog in breast CSCs were detected by quantitative polymerase chain reaction and western blotting. RNA interference (RNAi) was used in order to downregulate the expression of Oct4 and Nanog. Drug resistance and tumor?initiating capability following in vivo injection of MDA?MB?231 stem cells trans-duced with negative RNAi, Oct4 RNAi and Nanog RNAi were compared with that of MDA?MB?231 stem cells without siRNA transfection as a control group. In addition the capability of MDA?MB?231 breast cancer cells to initiate tumor formation in mice was compared with that of MDA?MB?231 stem cells. A paclitaxel inhibition test was also conducted in order to detect resistance of MDA?MB?231 breast cancer stem cells to this treatment. The MDA?MB?231 stem cells were revealed to exhibit elevated percentages of the cluster of differentiation (CD)44+CD24?/low subset, high tumorigenicity and resistance to chemotherapy, all of which are characteristic stem cell properties. In addition, the MDA?MB?231 stem cells were more tumorigenic in vivo. Furthermore, the breast CSCs also expressed high levels of the Oct4 and Nanog transcription factors. Therefore, downregulation of Oct4 or Nanog expression may reduce chemotherapeutic drug resistance and tumorigenicity in breast CSCs. In conclusion, Oct4 and Nanog expression may be a key factor in the development of resistance to chemotherapy and tumor growth of breast CSCs. This finding indicates that Oct4 or Nanog?targeted therapy may be a promising means of overcoming resistance to chemotherapy and inhibiting tumor growth in breast cancer treatment.
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MANAGEMENT OF ENDOCRINE DISEASE: Effects of telecare intervention for glycemic control in Type 2 Diabetes: A systematic review and meta-analysis of randomized controlled trials.
Eur. J. Endocrinol.
PUBLISHED: 09-18-2014
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Objective: To review published literature on the effects of telecare intervention in patients with Type 2 Diabetes and inadequate glycemic control. Design and methods: A review of randomized controlled trials (RCTs) on telecare intervention in patients with type 2 diabetes, and a search of electronic databases such as The Cochrane Library, PubMed, EBSCO, CINAHL, Science Direct, Journal of Telemedicine and Telecare, and China National Knowledge Infrastructure (CNKI), were conducted from December 8-16, 2013. Two evaluators independently selected and reviewed the eligible studies. Change in glycosylated hemoglobin (HbA1c), fasting plasma glucose (FPG), postprandial plasma glucose (PPG), Body Mass Index (BMI), and body weight were analyzed. Results: An analysis of 18 studies with 3,798 subjects revealed that telecare significantly improved the management of diabetes. Mean HbA1c values reduced by (-0.54; 95% confidence interval [CI], -0.75 to -0.34; P < 0.05), mean FPG levels by (-9.00 mg/dL; 95% CI, -17.36 to -0.64; P = 0.03), and mean PPG levels reduced by (-52.86 mg/dL; 95% CI, -77.13 to -28.58; P < 0.05) than the group receiving standard care. Meta-regression and subgroup analyses showed that study location, sample size, and treatment monitoring techniques were sources of heterogeneity. Conclusions: Patients monitored by telecare showed significant improvement in glycemic control in type 2 diabetes than those monitored by routine follow-up. Significant reduction in HbA1c was associated with Asian populations, small sample size, and telecare, and those with patients with baseline HbA1c greater than 8.0%.
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A luminescent microporous metal-organic framework with highly selective CO? adsorption and sensing of nitro explosives.
Inorg Chem
PUBLISHED: 08-29-2014
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A luminescent microporous metal-organic framework based on a ?-electron-rich tricarboxylate ligand and an In(3+) ion has been solvothermally obtained and characterized and exhibits highly selective CO2 adsorption over CH4 and N2 gases and selective sensing of the nitro explosive 2,4,6-trinitrophenol.
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ArhGAP30 promotes p53 acetylation and function in colorectal cancer.
Nat Commun
PUBLISHED: 08-26-2014
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Covalent modification adding acetyl groups to the C terminus of the p53 protein has been suggested to be required for its functional activation as a tumour suppressor. However, it remains largely unknown how p53 acetylation is deregulated in colorectal cancer (CRC), which is the third most commonly diagnosed cancer worldwide. Here we show that ArhGAP30, a Rho GTPase-activating protein, is a pivotal regulator for p53 acetylation and functional activation in CRC. ArhGAP30 binds to p53 C-terminal domain and P300, facilitating P300-mediated acetylation of p53 at lysine 382. ArhGAP30 expression is required for p53 activation upon DNA damage stress, and the level of ArhGAP30 correlates with p53 acetylation and functional activation in CRC tissues. Moreover, low level of ArhGAP30 expression associates with poor survival of CRC patients. In summary, ArhGAP30 is required for p53 acetylation and functional activation in CRC, and the expression of ArhGAP30 is a potential prognostic marker for CRC.
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Blood Neutrophil-Lymphocyte Ratio Predicts Survival in Patients with Advanced Pancreatic Cancer Treated with Chemotherapy.
Ann. Surg. Oncol.
PUBLISHED: 08-26-2014
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Although a high neutrophil-to-lymphocyte ratio (NLR) has been reported to be a predictor of poor survival in patients with pancreatic cancers, its prognostic role in patients with advanced pancreatic cancer undergoing chemotherapy remains unclear. This study was performed to determine the prognostic role of NLR in patients with advanced pancreatic cancer undergoing chemotherapy.
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A Huaier polysaccharide inhibits hepatocellular carcinoma growth and metastasis.
Tumour Biol.
PUBLISHED: 08-25-2014
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This study was carried out to evaluate the effects of a Huaier polysaccharide (TP-1) on the tumor growth and immune function in hepatocellular carcinoma (HCC) H22-based mouse in vivo. Results showed that TP-1 was capable of repressing transplanted H22 solid hepatic tumor cell growth in vivo, prolonging the live time of mice bearing ascetic H22 tumors, and repressing the pulmonary metastasis of H22-bearing mice. Moreover, the relative weight of immune organ (spleen and thymus) and lymphocyte proliferation were improved after TP-1 treatment. Furthermore, the treatment with TP-1 could promote immune-stimulating serum cytokines, such as IL-2 and IFN-?, but inhibit immune-suppressing serum cytokines IL-10 secretion in H22-bearing mice. Besides, the percentage of CD4+ T cells and NK cells was increased, whereas the number of CD8+ T cells decreased in tumor-bearing mice following TP-1 administration. In addition, this compound displayed little toxic effects to major organ of tumor-bearing mice at the therapeutic dose, such as the liver and kidney. This experimental finding suggested that TP-1 exhibited prominent antitumor activities in vivo via enhancement of host immune system function in H22 tumor-bearing mice. This product could be developed individually as a safe and potent biological response modifier for HCC therapy.
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Profilin-1 suppresses tumorigenicity in pancreatic cancer through regulation of the SIRT3-HIF1? axis.
Mol. Cancer
PUBLISHED: 08-07-2014
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Tumor cells exhibit abnormal actin remodeling profiles, which involve the altered expressions of several important actin-binding proteins. Profilin1 (Pfn1), originally identified as an actin-associated protein, has been linked to several human malignancies. Our recent studies suggested that Pfn1 facilitates apoptosis in pancreatic cancer cells. Here, we investigated the exact role of Profilin1 (Pfn1) in pancreatic adenocarcinoma (PDAC) and the underlying mechanisms.
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[Identification of vibration characteristics in infrared spectrum of polybrominated diphenyl ethers congeners based on Br substituents].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 08-07-2014
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Structure optimization and frequency calculations of 209 kinds of PBDEs homologues were carried out at the B3LYP/6-31G(d) level based on density functional theory, the known experimental parameters of 4 PBDEs congeners were compared with the calculated values, and the average analog coefficient was 0. 989. The main vibration of DE and BDE-209 which contained only H atoms or Br substituent in the benzene ring was assigned due to their less interference for various infrared spectrum vibrations. C-Br bond vibration which was often coupled with other forms of vibration was not easy to distinguish between PBDEs congeners. With the characteristics of universality, strong vibration and identifiable, triangular breathing vibration of the benzene rings in the infrared spectrum was selected as a characteristic vibration among different PBDEs homologues, the analysis results showed that the ortho-substituted and para-substituted Br could significantly improve the vibration frequency of triangular breathing vibration, while the meta-substituted Br played the opposite role; For the low bromide, the more the substituents in ortho position, the higher the frequency, while for the high bromide homologues, simultaneous existence in ortho-position and para-position would have a higher frequency; With the same substituted location, the more the substituent in the same benzene ring, the higher the vibration frequency. SVM regression model was used to verify the relative relationship between Br substituent and triangle breathing vibration frequency of the benzene rings, and the simulation efficiency coefficient of model was 0. 956. The study provided a theoretical basis and guidance for actually testing and identifying PBDEs homologues in the environment.
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Microdynamics mechanism of thermal-induced hydrogel network destruction of poly(vinyl alcohol) in D2O studied by two-dimensional infrared correlation spectroscopy.
J Phys Chem B
PUBLISHED: 07-29-2014
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Microdynamics mechanism of thermal-induced hydrogel network destruction of poly(vinyl alcohol) (PVA) in D2O at heating (25-62 °C) was studied by in situ Fourier transform infrared (FTIR) spectroscopy combining with moving-window two-dimensional (MW2D) technique and two-dimensional (2D) correlation analysis. The temperature range of hydrogel destruction was determined within 34-52 °C by dynamic rheological test at first, and then also monitored by MW2D FTIR spectra. The motion of vs(-C-O-, microcrystals) was important in the entire hydrogel destruction process. The microdynamics mechanism of PVA molecular chains can be elaborated as follows: At 32 °C, the number of D2O molecules in the swollen amorphous remains unchanged. At 32-37 °C, more D2O molecules enter into the swollen amorphous region, and the groups of -C-O-, together with -CH2-, are partially hydrated. At 37 °C, the intramolecular or intermolecular hydrogen bonds of PVA are dissociated. The physical cross-linking points of hydrogel are broken due to the melting of PVA microcrystals. At 42 °C, the dissociated hydroxyls from PVA microcrystals rapidly integrate solid hydrogen bonds with D2O molecules. The groups of -C-O- and -CH- are completely hydrated by D2O simultaneously. At 45-55 °C, PVA molecules are surrounded by more D2O molecules. The partially hydrated -CH2- is completely hydrated, and all of the PVA molecules are fully dissolved in D2O.
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Pulmonary Embolism and Renal Vein Thrombosis in Patients with Nephrotic Syndrome: Prospective Evaluation of Prevalence and Risk Factors with CT.
Radiology
PUBLISHED: 07-28-2014
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Purpose To prospectively determine the prevalence of pulmonary embolism (PE) and renal vein thrombosis (RVT) with computed tomography (CT) and to identify markers predictive of PE and/or RVT in a large consecutive cohort of patients with nephrotic syndrome. Materials and Methods This study was approved by the local institutional review board, and all patients or their guardians provided written informed consent. Consecutive patients with nephrotic syndrome (24-hour urine protein > 3.5 g) underwent combined CT pulmonary angiography for PE and renal CT venography for RVT. Prevalence of PE and/or RVT was estimated for different ages, sexes, and histopathologic types of nephrotic syndrome. Multivariate analysis was used to determine independent predictors for PE and/or RVT in patients with nephrotic syndrome. Results There were 512 patients in the study cohort (331 male patients, 181 female patients; mean age, 37 years ± 17 [standard deviation]; range, 9-81 years), including 80 children. One hundred eighty (35%) of 512 patients had PE and/or RVT, with PE the more common condition (85% [153 of 180]). PE was associated with RVT in 85 (56%) of 153 patients and was isolated in 68 patients (44%). Most patients with PE (84% [128 of 153]) were asymptomatic. One hundred twelve (22%) of 505 patients had RVT. PE and/or RVT was found in 15 (19%) of 80 children with nephrotic syndrome, while 165 (38%) of 432 adult patients with nephrotic syndrome had PE and/or RVT (P = .001). Membranous nephropathy was the most common histopathologic type associated with PE and/or RVT (48% [88 of 183]). Membranous nephropathy, age greater than 60 years, high hemoglobin level, long prothrombin time, and high creatinine level were independent predictors of PE and/or RVT (P < .05 for all). Conclusion PE and RVT are common in patients with nephrotic syndrome. PE is more common than RVT, is most often asymptomatic, and is most frequently found in patients with membranous nephropathy. A high index of suspicion and a low threshold for diagnostic work-up is warranted in these patients. © RSNA, 2014 Online supplemental material is available for this article.
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Hemodynamic alterations in unilateral chronic middle cerebral artery stenosis patients and the effect of percutaneous transluminal angioplasty and stenting: a perfusion-computed tomography study.
Acta Radiol
PUBLISHED: 07-11-2014
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The Stenting and Aggressive Medical Management for Preventing Recurrent Stroke in Intracranial Stenosis (SAMMPRIS) trial indicated that most patients with symptomatic intracranial atherosclerosis are not good candidates for percutaneous transluminal angioplasty and stenting (PTAS) because of a higher complication risk than with conservative treatment. However, enrollment of SAMMPRIS patients was based on lesion severity only, without functional imaging.
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The combination of HTATIP2 expression and microvessel density predicts converse survival of hepatocellular carcinoma with or without sorafenib.
Oncotarget
PUBLISHED: 07-11-2014
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Our previous studies have demonstrated that sorafenib can promote the dissemination of hepatocellular carcinoma (HCC) through downregulation of HTATIP2, a suppressor of tumor growth and metastasis that is associated with inhibition of angiogenesis. Here, we investigated the predictive values of the HTATIP2 level and microvessel density (MVD) with or without sorafenib administration for HCC. Three independent cohorts were included. Using tissue microarray, we assessed the relationship between HTATIP2 expression/MVD and overall survival. The results showed that high HTATIP2 expression and a low MVD value were independent protective prognostic factors after curative HCC resection (297 cases/cohort 1); however, both parameters were converted to independent negative prognostic indicators for patients with postsurgical sorafenib treatment (69/143 cases/cohort 2; P<0.05 for all). This same relationship was observed in patients that received sorafenib treatment for advanced HCC (83 cases/cohort 3; efficacy measures and survival analyses, P<0.05 for all). Moreover, the combination of HTATIP2 and MVD had better power to predict patient death and disease recurrence (P<0.001 for both). We conclude that the combination of HTATIP2 and MVD predicts the converse survival of HCC with or without sorafenib intervention. Our findings can assist in the selection of candidates for personalized treatment with sorafenib.
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Effects of antioxidants on the microleakage of composite resin restorations after external tooth bleaching.
Eur J Dent
PUBLISHED: 06-27-2014
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To compare the effects of three antioxidants (sodium ascorbate, sodium ascorbate combined with a surfactant, and catalase) on the microleakage of composite resin restorations after external tooth bleaching with 10% carbamide peroxide.
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Disrupted small world networks in patients without overt hepatic encephalopathy: a resting state fMRI study.
Eur J Radiol
PUBLISHED: 06-24-2014
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To explore changes in functional connectivity and topological organization of brain functional networks in cirrhotic patients with minimal hepatic encephalopathy (MHE) and non hepatic encephalopathy (nonHE) and their relationship with clinical markers.
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Syrinx resolution is correlated with the upward shifting of cerebellar tonsil following posterior fossa decompression in pediatric patients with Chiari malformation type I.
Eur Spine J
PUBLISHED: 06-23-2014
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Chiari malformation type I (CMI) is characterized by deformed hindbrain. This study aimed to quantitatively evaluate the alterations in position of hindbrain after Posterior fossa decompression (PFD), and to identify the factors associated with syrinx resolution in pediatric patients with CMI.
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Image quality and radiation dose of lower extremity CT angiography at 70 kVp on an integrated circuit detector dual-source computed tomography.
Acta Radiol
PUBLISHED: 06-13-2014
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Despite the well-established requirement for radiation dose reduction there are few studies examining the potential for lower extremity CT angiography (CTA) at 70 kVp.
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Simvastatin inhibits inflammation in ischemia-reperfusion injury.
Inflammation
PUBLISHED: 06-11-2014
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Ischemia/reperfusion (I/R) is associated with leukocyte accumulation and tissue injury. The aim of this research was to investigate the protective effect of simvastatin on hind limb I/R inflammation and tissue damage. Mice were subjected to hind limb ischemic insult for 2 h and were simultaneously administered an intraperitoneal injection of simvastatin (5 mg/kg); this was followed by 36 h of reperfusion. Myeloperoxidase (MPO) levels in the muscles of the hind limb were determined. CXC chemokines and pro-inflammatory cytokines, such as macrophage inflammatory protein (MIP)-2, cytokine-induced neutrophil chemoattractant (KC), interleukin (IL)-6, tumor necrosis factor (TNF)-?, and P-selectin, were assessed using enzyme-linked immunosorbent assay (ELISA). Leukocyte rolling and adhesion in vitro was assessed to indicate leukocyte recruitment at the site of inflammation. Quantitative measurement of skeletal muscle tissue injury was performed. The fluorescent dye level in tissue and serum was used to determine hind limb vascular leakage and tissue edema after I/R. Systemic and differentiated leukocytes were also counted. Simvastatin significantly reduced MIP-2, KC, TNF-?, MPO, IL-6, and P-selectin levels compared to the sham group and I/R plus pretreatment with phosphate-buffered saline (PBS) group (P<0.05). Compared to the sham group and I/R plus PBS group, the I/R plus simvastatin group had attenuated inflammation, vascular leakage, and muscular damage (P<0.05). Simvastatin also significantly inhibited leukocyte rolling and adhesion compared to PBS (P<0.05). Our results suggest that simvastatin may be an effective protectant against tissue injury associated with I/R.
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(18)F-FDG PET/CT can be used to detect non-functioning pancreatic neuroendocrine tumors.
Int. J. Oncol.
PUBLISHED: 06-10-2014
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18F-Fluorodeoxyglucose positron emission tomo-graphy and computed tomography (18F-FDG PET/CT) has limited value in well-differentiated neuroendocrine tumors. The value of 18F-FDG PET/CT in non-functioning pancreatic neuroendocrine tumors, which are often poorly differentiated, malignant, and present at an advanced stage, was also thought to be limited. This study was performed to evaluate the clinical value of 18F-FDG PET/CT in assessing non-functioning pancreatic neuroendocrine tumors. From January 2010 to February 2014, a comparable large cohort of patients (31 cases) with non-functioning pancreatic neuroendocrine tumors from Shanghai Cancer Center underwent 18F-FDG PET/CT scans. Demographics and clinical characteristics were retrospectively collected and analyzed for all the patients. Twenty-eight of 31 (90.3%) patients with non-functioning endocrine pancreatic tumors had an elevated 18F-FDG uptake (SUVmax ?2.5). In addition, 18F-FDG PET/CT visualized 38 of 42 (90.5%) distant metastatic lesions. The 18F-FDG uptake had significant association with tumor size (P=0.012) and TNM stage (P=0.004). The application of 18F-FDG PET/CT has changed the management of 8 cases (8/31, 25.8%). In conclusion, 18F?FDG PET/CT plays an important role in detecting and staging non-functioning pancreatic neuroendocrine tumors.
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Altered modular organization of functional connectivity networks in cirrhotic patients without overt hepatic encephalopathy.
Biomed Res Int
PUBLISHED: 06-05-2014
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Minimal hepatic encephalopathy (MHE) is associated with changes in functional connectivity. To investigate the patterns of modular changes of the functional connectivity in the progression of MHE, resting-state functional magnetic resonance imaging was acquired in 24 MHE patients, 31 cirrhotic patients without minimal hepatic encephalopathy (non-HE), and 38 healthy controls. Newman's metric, the modularity Q value, was maximized and compared in three groups. Topological roles with the progression of MHE were illustrated by intra- and intermodular connectivity changes. Results showed that the Q value of MHE patients was significantly lower than that of controls (P < 0.01) rather than that of non-HE patients (P > 0.05), which was correlated with neuropsychological test scores rather than the ammonia level and Child-Pugh score. Less intrasubcortical connections and more isolated subcortical modules were found with the progression of MHE. The non-HE patients had the same numbers of connect nodes as controls and had more hubs compared with MHE patients and healthy controls. Our findings supported that both intra- and intermodular connectivity, especially those related to subcortical regions, were continuously impaired in cirrhotic patients. The adjustments of hubs and connector nodes in non-HE patients could be a compensation for the decreased modularity in their functional connectivity networks.
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An AIE-active luminophore with tunable and remarkable fluorescence switching based on the piezo and protonation-deprotonation control.
Chem. Commun. (Camb.)
PUBLISHED: 05-30-2014
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A novel luminophore TPENSOH was facilely synthesized from the building blocks of tetraphenylethylene and 6-hydroxylbenzothiazole and exhibited unique AIE properties. This new dye was found to show a remarkable and reversible four-color switching based on a single molecule in the solid state.
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Multidetector computed tomography of superior mesenteric artery: anatomy and pathologies.
Can Assoc Radiol J
PUBLISHED: 05-27-2014
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The recent introduction of multidetector computed tomography scanners has significantly improved computed tomography angiographic (CTA) applications, especially for the evaluation of medium- and small-arterial structures. CTA of the superior mesenteric artery has been reported previously. However, there have been few systematic and detailed reviews of the superior mesenteric artery pathologies that use CTA. The purpose of this pictorial essay is mainly to review the various superior mesenteric artery pathologies at CTA with our own experiences.
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Sensitive naked-eye detection of Hg2+ based on the aggregation and filtration of thymine functionalized vesicles caused by selective interaction between thymine and Hg2+.
Analyst
PUBLISHED: 05-16-2014
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We report a sensitive, selective and low-cost method for the naked-eye detection of Hg(2+). The principle is based on rapid interaction between functionalized PDA vesicles and Hg(2+), which leads to obvious aggregation of vesicles. Furthermore, using only a simple filtration process, without using any other color indicator or specialized equipment, a higher detection sensitivity for Hg(2+) (0.1 ?M) than chromophoric colorimetric sensors (approximately 1-100 ?M) was obtained.
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[Natural history of scoliosis after posterior fossa decompression in patients with Chiari malformation/syringomyelia].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 04-12-2014
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To explore the natural history of scoliosis after posterior fossa decompression (PFD) in patients with Chiari malformation/syringomyelia and examine the risk factors associated with curve progression.
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Low tube voltage and low contrast material volume cerebral CT angiography.
Eur Radiol
PUBLISHED: 04-09-2014
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To evaluate the image quality, radiation dose and diagnostic accuracy of low kVp and low contrast material volume cerebral CT angiography (CTA) in intracranial aneurysm detection.
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Quantitative measurements of brain iron deposition in cirrhotic patients using susceptibility mapping.
Acta Radiol
PUBLISHED: 03-21-2014
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Susceptibility-weighted imaging (SWI) has been used to detect micro-bleeds and iron deposits in the brain. However, no reports have been published on the application of SWI in studying iron changes in the brain of cirrhotic patients.
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Feasibility of prospectively ECG-triggered high-pitch coronary CT angiography with 30 mL iodinated contrast agent at 70 kVp: initial experience.
Eur Radiol
PUBLISHED: 03-19-2014
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To evaluate the feasibility, image quality and radiation dose of prospectively ECG-triggered high-pitch coronary CT angiography (CCTA) with 30 mL contrast agent at 70 kVp.
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Can virtual non-enhanced CT be used to replace true non-enhanced CT for the detection of palpable cervical lymph nodes? A preliminary study.
Jpn J Radiol
PUBLISHED: 03-04-2014
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To investigate the potential of virtual non-contrast CT (VNCT) from dual-energy CT to replace true nonenhanced CT (TNCT) for the detection of enlarged cervical lymph nodes.
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Risk factors for critical disease and death from hand, foot and mouth disease.
Pediatr. Infect. Dis. J.
PUBLISHED: 03-01-2014
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There has been a high mortality and morbidity rate of critical and fatal patients from hand, foot and mouth disease (HFMD) in China in recent. Causes for development of critical and fatal disease remain unclear.
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A robust automated markerless registration framework for neurosurgery navigation.
Int J Med Robot
PUBLISHED: 02-26-2014
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The registration of a pre-operative image with the intra-operative patient is a crucial aspect for the success of navigation in neurosurgery.
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Abnormal functional connectivity within the default mode network in patients with HBV-related cirrhosis without hepatic encephalopathy revealed by resting-state functional MRI.
Brain Res.
PUBLISHED: 02-21-2014
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By means of "task free" resting-state functional magnetic resonance imaging (rs-fMRI), abnormal functional connectivity (FC) of the default mode network (DMN) in cirrhotic patients with hepatic encephalopathy (HE) has been reported; however, little is known about the changes of DMN in cirrhotic patients without overt or minimal HE. The aim of this study was to investigate whether there was a disruption of the FC within the DMN in patients with hepatitis B virus (HBV)-related cirrhosis without any signs of HE. Fifty one patients with HBV-related cirrhosis without HE and 61 age- and gender-matched healthy controls underwent the rs-fMRI. Seed-based region-to-region FC was used to analyze the connectivity between each pair of regions within the DMN, including posterior cingulate cortex (PCC), medial prefrontal cortex (mPFC), hippocampal formation (HF), inferior parietal cortex (IPC), and medial temporal lobe (MTL). Pearson correlation analysis was performed between the abnormal FC strength within the DMN and venous blood ammonia levels in patients. Compared with the controls, patients with HBV-related cirrhosis without HE demonstrated significantly decreased region-to-region FC between the mPFC and bilateral MTL, right HF, and left IPC, as well as between the right MTL and left IPC, right HF, and PCC. A significant negative relationship was observed between blood ammonia levels and connectivity strength between the mPFC and left IPC in patients. These results suggest that patients with HBV-related cirrhosis without HE had disrupted functional connectivty within the DMN, even before the appearance of minimal HE.
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[The clinical significance of preoperative enteral immune nutrition in patients with malignant gastrointestinal tumors].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 02-18-2014
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To study the impact of preoperative enteral immune nutrition on patients with malignant gastrointestinal tumors.
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Altered brain functional connectivity in hemodialysis patients with end-stage renal disease: a resting-state functional MR imaging study.
Metab Brain Dis
PUBLISHED: 02-13-2014
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The changes of whole brain functional connectivity in hemodialysis (HD) patients with end-stage renal disease (ESRD) are still unclear, which may be associated with multiple factors, such as elevated neurotoxins, anemia, and side effects of hemodialysis. Resting-state functional magnetic resonance imaging (rs-fMRI) data of 71 patients (43 males, 28 females; mean age, 33.4?±?9.4 years) and 43 age- and gender-matched healthy volunteers (29 males, 14 females; mean age, 30.6?±?8.8 years) were acquired. Neuropsychological tests including number connection test type A (NCT-A), digit symbol test (DST), line-tracing test (LTT), serial-dotting test (SDT), self-rating depression scale (SDS) and self-rating anxiety scale (SAS) were used to evaluate cognitive and psychiatric conditions in all subjects. Blood biochemistry tests including serum creatinine levels, blood urea, hematocrit, and Ca(2+) level were taken in HD patients. Forty-two connections significantly different between HD patients with ESRD and controls were found (all P?
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LSD1 sustains pancreatic cancer growth via maintaining HIF1?-dependent glycolytic process.
Cancer Lett.
PUBLISHED: 02-12-2014
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The histone demethylase LSD1 (lysine specific demethylase 1) plays an important role in the epigenetic regulation of gene transcription. Our study investigated the role of LSD1 in pancreatic cancer and demonstrated that LSD1 was significantly up-regulated in pancreatic cancer patient tissue samples, and elevated LSD1 protein levels positively correlated with overall survival of pancreatic cancer patients. Using in vitro and in vivo models, we demonstrated that knock-down of LSD1 repressed proliferation and tumorigenicity of pancreatic cancer cells. Mechanistically, our study demonstrated that LSD1 synergized with HIF1? (hypoxia inducible factor-1?) in maintaining glycolytic process, which fueled pancreatic cancer uncontrolled proliferation.
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High-pitch computed tomography pulmonary angiography with iterative reconstruction at 80 kVp and 20 mL contrast agent volume.
Eur Radiol
PUBLISHED: 02-08-2014
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To evaluate the image quality, radiation dose and diagnostic accuracy of 80kVp, high-pitch CT pulmonary angiography (CTPA) with iterative reconstruction using 20 ml of contrast agent.
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Resting-state functional magnetic resonance imaging in hepatic encephalopathy: current status and perspectives.
Metab Brain Dis
PUBLISHED: 02-06-2014
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Hepatic encephalopathy (HE) is a neuropsychiatric syndrome which develops in patients with severe liver diseases and/or portal-systemic shunting. Minimal HE, the earliest manifestation of HE, has drawn increasing attention in the last decade. Minimal HE is associated with a series of brain functional changes, such as attention, working memory, and so on. Blood oxygen level dependent (BOLD) functional MRI (fMRI), especially resting-state fMRI has been used to explore the brain functional changes of HE, yielding important insights for understanding pathophysiological mechanisms and functional reorganization of HE. This paper briefly reviews the principles of BOLD fMRI, potential applications of resting-state fMRI with advanced post-processing algorithms such as regional homogeneity, amplitude of low frequency fluctuation, functional connectivity and future research perspective in this field.
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A preoperative serum signature of CEA(+) /CA125(+) /CA19-9???1000 U/mL indicates poor outcome to pancreatectomy for pancreatic cancer.
Int. J. Cancer
PUBLISHED: 02-05-2014
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Pancreatectomy is associated with significant morbidity and unpredictable outcome, with few diagnostic tools to determine, which patients gain the most benefit from this treatment, especially before the operation. This study aimed to define a preoperative signature panel of serum markers to indicate response to pancreatectomy for pancreatic cancer. Over 1000 patients with pancreatic cancer treated at two independent high-volume institutions were included in this study and were divided into three groups, including resected, locally advanced and metastatic. Eight serum tumor markers most commonly used in gastrointestinal cancers were analyzed for patient outcome. Preoperative CA19-9 independently indicated surgical response in pancreatic cancer. Patients with CA19-9 ?1000 U/mL generally had a poor surgical benefit. However, a subset of these patients still achieved a survival advantage when CA19-9 levels decreased postoperatively. CEA and CA125 in the presence of CA19-9 ?1000 U/mL could independently predict the non-decrease of CA19-9 postoperatively. The combination of the three markers was useful for predicting a worse surgical outcome with a median survival of 5.1 months vs. 23.0 months (p?
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Hand-assisted thoracoscopic surgery for pulmonary metastasectomy through sternocostal triangle access: superiority in detection of non-imaged pulmonary nodules.
Sci Rep
PUBLISHED: 01-29-2014
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Hand-Assisted Thoracoscopic Surgery for pulmonary metastasectomy through sternocostal triangle access allows manual palpation of both lungs, thus permitting effective treatment of lung metastases. In our research, 62 patients from November 2001 to January 2012 underwent our Hand-Assisted Thoracoscopic Surgery procedures for pulmonary metastasectomy. Clinical data, including the number of pulmonary metastases determined by Computed Tomography/Positron Emission Tomography-Computed Tomography, surgical findings and survival data of these patients were collected. We found that the median follow-up time was 23.7 months (range 2.4 to 85.6 months). 30 cases of them had post-operative recurrences and the median disease-free survival period was 27.4 months. For Computed Tomography scan, the overall sensitivity for proved metastases was 63% (115/182). 67 non-imaged malignant nodules were palpated and removed in 14 cases. For Positron Emission Tomography-Computed Tomography scan, the overall sensitivity was 66% (79/120). 41 non-imaged malignant nodules were palpated and removed in 12 cases. This study show that the Hand-Assisted Thoracoscopic Surgery provides an easier way for routine bilateral pleural exploration, and thus is critical and effective in detection of non-imaged malignant pulmonary metastases, which might contribute to long-term disease-free survival.
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Aberrant default-mode functional connectivity in patients with end-stage renal disease: a resting-state functional MR imaging study.
Radiology
PUBLISHED: 01-24-2014
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To investigate the functional connectivity of the default-mode network (DMN) in patients with end-stage renal disease (ESRD) by using independent component analysis of resting-state functional magnetic resonance (MR) imaging and to correlate these DMN connectivity changes with neuropsychological test results and clinical biomarkers.
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Xenon-enhanced dual-energy CT lung ventilation imaging: techniques and clinical applications.
AJR Am J Roentgenol
PUBLISHED: 01-24-2014
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The purpose of this article is to review the techniques, clinically relevant potential applications, and limitations of xenon-enhanced dual-energy CT of the chest.
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Molecular mechanism underlying lymphatic metastasis in pancreatic cancer.
Biomed Res Int
PUBLISHED: 01-22-2014
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As the most challenging human malignancies, pancreatic cancer is characterized by its insidious symptoms, low rate of surgical resection, high risk of local invasion, metastasis and recurrence, and overall dismal prognosis. Lymphatic metastasis, above all, is recognized as an early adverse event in progression of pancreatic cancer and has been described to be an independent poor prognostic factor. It should be noted that the occurrence of lymphatic metastasis is not a casual or stochastic but an ineluctable and designed event. Increasing evidences suggest that metastasis-initiating cells (MICs) and the microenvironments may act as a double-reed style in this crime. However, the exact mechanisms on how they function synergistically for this dismal clinical course remain largely elusive. Therefore, a better understanding of its molecular and cellular mechanisms involved in pancreatic lymphatic metastasis is urgently required. In this review, we will summarize the latest advances on lymphatic metastasis in pancreatic cancer.
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Sagittal spinopelvic alignment in adolescents associated with Scheuermann's kyphosis: a comparison with normal population.
Eur Spine J
PUBLISHED: 01-20-2014
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Spinopelvic alignment is increasingly considered as a main factor in the energy-efficient posture of the individual in normal and pathological status. However, the spinopelvic characteristics in Scheuermann's kyphosis (SK) are poorly defined in the literature. The purpose of this study was to determine whether differences of the spinopelvic parameters exist between adolescents with SK and age-matched normal controls.
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Effects of deferoxamine on the repair ability of dental pulp cells in vitro.
J Endod
PUBLISHED: 01-17-2014
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In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia. The purpose of this study was to investigate the effects of DFO on the repair ability of DPCs.
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Combined effect of five single nucleotide polymorphisms related to IL23/Th17 pathway in the risk of psoriasis.
Immunogenetics
PUBLISHED: 01-14-2014
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Psoriasis is a common immune-mediated inflammatory skin disease with strong genetic components, in which the IL23/Th17 pathway has been implicated. To explore the effective role in psoriasis, we genotyped five single nucleotide polymorphisms in genes related to IL23/Th17 pathway in 14,929 Han Chinese samples. A Bonferroni-corrected significant single-variant association was identified between rs1512970 within IL21 (odds ratio (OR)?=?1.07, 95 % confidence interval (CI)?=?1.02-1.13, P?=?4.94?×?10(-03)). We failed to validate rs744166 (OR?=?1.06, 95 % CI?=?1.01-1.11, P?=?1.52?×?10(-02)) and other three SNPs (P?=?2.48?×?10(-01)???1.27?×?10(-02)) to meet the single-variant association significance threshold. However, we found that their combined effect substantially contributed to the risk of psoriasis in our sample (P?=?3.91?×?10(-07)) and the highest score group conferred the largest risk effect size (OR?=?1.22, 95 % CI?=?1.11-1.34, P?=?1.85?×?10(-05)). Our results implicate the ethnic heterogeneity in the susceptibility of psoriasis and suggest common variants with weak effect in IL23/Th17 pathway, which do not show significance in conventional single-variant association study, may contribute to the risk of psoriasis. This study sheds light on the important role of IL23/Th17 pathway in the susceptibility of psoriasis.
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Abnormal distribution of peripheral lymphocyte subsets induced by PDAC modulates overall survival.
Pancreatology
PUBLISHED: 01-12-2014
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The impairment of the immune system is prevalent in patients with malignancies, including pancreatic ductal adenocarcinoma (PDAC). The present study aimed to evaluate alternations of peripheral lymphocyte subsets in patients with PDAC, and also to assess the prognostic value of observed changes.
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Cancer statistics: current diagnosis and treatment of pancreatic cancer in Shanghai, China.
Cancer Lett.
PUBLISHED: 01-11-2014
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A multi-center population-based study in Shanghai, China was performed to explore the implications for the management of pancreatic cancer by comparing diagnosis and survival rates. Novel imaging modalities including MRI (13.9%), PET/CT (1.8%), and EUS (5.6%) were not widely used in our population. Only 39.7% of cases were histologically verified (surgery with histologic diagnosis 31.0%, cytological diagnosis 8.7%, surgery without histologic diagnosis 12.1%, and clinical diagnosis 48.2%). Overall, 30.0% of patients underwent curative-intent operation, and only 9.8% of patients received comprehensive treatment. The prognosis of pancreatic cancer patients was significantly better for patients who were treated in high-volume centers than in low-volume centers. We propose that more effort should be put on novel diagnostic modalities, histological confirmation, and comprehensive treatment in China. Multidisciplinary teams specialized in pancreatic cancer therapy in high-volume centers are urgently needed.
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Metabolic tumour burden assessed by ¹?F-FDG PET/CT associated with serum CA19-9 predicts pancreatic cancer outcome after resection.
Eur. J. Nucl. Med. Mol. Imaging
PUBLISHED: 01-02-2014
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Tumour burden is one of the most important prognosticators for pancreatic ductal adenocarcinoma (PDAC). The aim of this study was to investigate the predictive significance of metabolic tumour burden measured by (18)F-FDG PET/CT in patients with resectable PDAC.
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Image quality and radiation dose of lower extremity CT angiography using 70 kVp, high pitch acquisition and sinogram-affirmed iterative reconstruction.
PLoS ONE
PUBLISHED: 01-01-2014
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The purpose of this study was to assess image quality and radiation dose of lower extremity CT angiography (CTA) with 70 kVp, high pitch acquisition and sinogram-affirmed iterative reconstruction (SAFIRE).
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A meta-analysis of red yeast rice: an effective and relatively safe alternative approach for dyslipidemia.
PLoS ONE
PUBLISHED: 01-01-2014
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To explore whether red yeast rice is a safe and effective alternative approach for dyslipidemia.
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Photothermal therapy of cancer cells using novel hollow gold nanoflowers.
Int J Nanomedicine
PUBLISHED: 01-01-2014
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This article presents a new strategy for fabricating large gold nanoflowers (AuNFs) that exhibit high biological safety under visible light and very strong photothermal cytotoxicity to HeLa cells under irradiation with near-infrared (NIR) light. This particular type of AuNF was constructed using vesicles produced from a multiamine head surfactant as a template followed by depositing gold nanoparticles (AuNPs) and growing their crystallites on the surface of vesicles. The localized surface plasmon-resonance spectrum of this type of AuNF can be easily modulated to the NIR region by controlling the size of the AuNFs. When the size of the AuNFs increased, biosafety under visible light improved and cytotoxicity increased under NIR irradiation. Experiments in vitro with HeLa cells and in vivo with small mice have been carried out, with promising results. The mechanism for this phenomenon is based on the hypothesis that it is difficult for larger AuNFs to enter the cell without NIR irradiation, but they enter the cell easily at the higher temperatures caused by NIR irradiation. We believe that these effects will exist in other types of noble metallic NPs and cancer cells. In addition, the affinity between AuNPs and functional biomolecules, such as aptamers and biomarkers, will make this type of AuNF a good recognition device in cancer diagnosis and therapy.
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Ligamentum teres hepatis patch enhances the healing of pancreatic fistula after distal pancreatectomy.
HBPD INT
PUBLISHED: 12-11-2013
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Pancreatic fistula is one of the most common complications after the distal pancreatectomy. Many methods have been tried to solve the problem, but no one is optimal, especially for the soft pancreatic stump cases. This study used ligamentum teres hepatis as a patch to cover the pancreatic stump. Between October 2010 and December 2012, seventy-seven patients who had undergone distal pancreatectomy with a soft pancreatic stump were divided into two groups: group A (n=39, patients received conventional ligated main pancreatic duct method) and group B (n=38, patients underwent a coverage procedure). Patients in group A had a longer recovery from postoperative pancreatic fistula than those in group B (16.4+/-3.5 vs 10.8+/-1.6 days, P<0.05). The coverage procedure with ligamentum teres hepatis is a safe, effective and convenient method for patients with a soft pancreas remnant during distal pancreatectomy.
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Prevalence and types of coronary to pulmonary artery fistula in a Chinese population at dual-source CT coronary angiography.
Acta Radiol
PUBLISHED: 11-26-2013
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Coronary to pulmonary artery fistula (CPAF) is rare; reports on the prevalence and types of CPAF in a large cohort of patients are scarce.
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Astrocyte elevated gene-1 (AEG-1) shRNA sensitizes Huaier polysaccharide (HP)-induced anti-metastatic potency via inactivating downstream P13K/Akt pathway as well as augmenting cell-mediated immune response.
Tumour Biol.
PUBLISHED: 11-19-2013
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Astrocyte elevated gene-1 (AEG-1) is an important force in the development and progression of hepatocellular carcinoma (HCC). To extend our study, we examined here the role of AEG-1 in anti-metastatic effects of Huaier polysaccharide (HP) on the human HCC MHCC97-H cell line. AEG-1 shRNA contributed to the anti-proliferation effect of HP on MHCC97-H cells. Furthermore, results of Transwell insert chambers showed that low expression of AEG-1 could effectively facilitate HP to suppress MHCC97-H cell migration and invasion. We achieved this by reducing phosphoinositide 3-kinases (P13K) and phosphorylated Akt (pAkt) expression as well as enhancing natural killer (NK) cell activity. Taken together, our data strongly suggested that AEG-1 shRNA could block the carcinogenesis and progression of MHCC97-H cells and highlight the therapeutic potential of HP in HCC treatment, at least by part, by inhibiting the activation of the PI3K/Akt pathway and enhancing the NK cell-mediated immune response. These findings may provide a new strategy for HCC treatment.
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The Delivery of Triamterene by Cucurbit[7]uril: Synthesis, Structures and Pharmacokinetics Study.
Mol. Pharm.
PUBLISHED: 11-04-2013
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In recent years, cucurbit[7]uril (CB[7]) has attracted great attention in drug delivery. Though the effect of CB[7] in enhancing the solubility of water insoluble drugs has been validated, the underlying mechanism remains poorly understood, particularly at a molecular level. This study is designed to evaluate a CB[7]-based pharmaceutical formulation to improve solubility and bioavailability of triamterene (a mild potassium-sparing diuretic). Two polymorphs of triamterene@CB[7] were obtained, and their crystal structures were determined by single crystal X-ray diffraction. The CB[7] molecule forms a stable host-guest complex with triamterene (Ka = 1.69 ± 0.34 × 10(4) M(-1)) in aqueous solution (pH = 1.0). The results of dissolution study demonstrate that the apparent solubility value of triamterene@CB[7] complex in 0.1 M HCl is 1.6 times as large as that of triamterene, while free triamterene was released from triamterene@CB[7] complex in phosphate buffer of pH 6.8. Pharmacokinetic studies in rats reveal that the AUC0-? value of triamterene@CB[7] complex increases 2.8-fold compared with that of free triamterene, and t1/2 is prolonged from 1.42 to 2.61 h (P < 0.05) after oral administration. The increased solubility and oral bioavailability are attributed to the formation of a hydrophilic capsule composed of two CB[7] molecules, in which two insoluble triamterene molecules are encapsulated. These results demonstrate that triamterene@CB[7] complex is a stable and effective pharmaceutical formulation.
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Mutation abundance affects the efficacy of EGFR tyrosine kinase inhibitor readministration in non-small-cell lung cancer with acquired resistance.
Med. Oncol.
PUBLISHED: 11-03-2013
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There is no consensus in the salvage treatment for non-small-cell lung cancer (NSCLC) with acquired resistance to primary epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs). Fifty-one consecutive EGFR-mutated NSCLC patients with TKI retreatment after acquired resistance were enrolled in this study. The quantitation of mutation abundance was performed by real-time fluorescent quantitative PCR. The correlation between mutation abundance and outcomes of readministrated TKI was analyzed by survival analysis. Patients with high (H) mutation abundance (24/51) had a significantly (log-rank, P < 0.05) longer (5.27-2.53 months) median progression-free survival (PFS), compared with the low (L) abundance group (27/51), whereas the median overall survival showed no difference (21.00-18.20 months, log-rank P = .403) between the two groups. Objective response and disease control rates in group H and group L regarding the second round TKI treatment were 8.3, 70.8 and 0, 48.1 %, respectively. Groupings with different mutation abundances were significantly associated with PFS under multivariate Cox proportional hazards regression model [hazard ratio (HR) for group H vs. L, 0.527; P = .036]. Mutation abundance affects the efficacy of EGFR-TKIs readministration in NSCLC with acquired resistance. The quantitative mutation abundance of EGFR may be a potential predictor for selecting optimal patients to readministrate EGFR-TKIs after acquired resistance to primary TKI.
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A highly stable dynamic fluorescent metal-organic framework for selective sensing of nitroaromatic explosives.
Chem. Commun. (Camb.)
PUBLISHED: 10-23-2013
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A dynamic fluorescent metal-organic framework has been constructed using triphenylene-2,6,10-tricarboxylate and Tb(3+) as building blocks, which exhibits guest-responsive structural dynamism and selective sensing of nitroaromatic explosives.
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Huaier polysaccharides suppresses hepatocarcinoma MHCC97-H cell metastasis via inactivation of EMT and AEG-1 pathway.
Int. J. Biol. Macromol.
PUBLISHED: 10-19-2013
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We have recently reported that astrocyte elevated gene-1 (AEG-1) might be an epithelial-mesenchymal transition (EMT) associated biomarker in human hepatocellular carcinoma (HCC), and play an important role in the progression of hepatocellular carcinoma. To extend our study, we examined here the anti-invasive and metastatic effects of Huaier polysaccharide (HP) on human HCC cell line MHCC97-H and explored its possible mechanism of action. Treatment with HP dose-dependently inhibited the proliferation, adhesion, migration and invasion of MHCC97-H cells in vitro. This was achieved not only by reducing the expression of AEG-1 and N-cadherin, but also by enhancing E-cadherin expression. Therefore, these data suggested that HP can inhibit the growth and metastatic potential of MHCC97-H cells through modulation of the AEG-1/EMT pathway.
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Enantioselective Recognition and Separation of Racemic 1-Phenylethanol by a Pair of 2D Chiral Coordination Polymers.
Inorg Chem
PUBLISHED: 10-01-2013
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A pair of 2D chiral coordination polymers were constructed through the self-assembly of a chiral metal-camphor-10-sulfonate salt and a bidentate linker, which show selective inclusion of S and R enantiomers of 1-phenylethanol respectively with an enantioselectivity of 9:1.
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Novel CT-guided coil localization of peripheral pulmonary nodules prior to video-assisted thoracoscopic surgery: a pilot study.
Acta Radiol
PUBLISHED: 09-27-2013
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Small peripheral lung nodules detected on computed tomography (CT) scans are often difficult to find during thoracoscopic resection, and the present localizing techniques are inefficient or impractical.
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Astrocyte elevated gene-1 is a novel biomarker of epithelial-mesenchymal transition and progression of hepatocellular carcinoma in two China regions.
Tumour Biol.
PUBLISHED: 09-25-2013
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Astrocyte elevated gene-1 (AEG-1) is involved in important biological processes including cell invasion, metastasis, and carcinogenesis. However, its clinical significance has remained largely unknown in hepatocellular carcinoma. Here, specimens from 144 patients with hepatocellular carcinomas in Beijing and Heilongjiang regions were investigated by immunohistochemical staining for AEG-1, vimentin, and E-cadherin expressions. A clinicopathological study revealed that AEG-1 expression level in tumor cells was significantly correlated with TNM stage (P?=?0.001) and Edmonson grade (P?
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Brain regional homogeneity changes following transjugular intrahepatic portosystemic shunt in cirrhotic patients support cerebral adaptability theory-A resting-state functional MRI study.
Eur J Radiol
PUBLISHED: 08-06-2013
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The exact neuro-pathophysiological effect of transjugular intrahepatic portosystemic shunt (TIPS) on brain function remains unclear. The purpose of this study was to investigate the longitudinal brain activity changes in cirrhotic patients with TIPS insertion using resting-state functional MRI (fMRI) with regional homogeneity (ReHo) method.
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Discrimination of cis-trans isomers by dinuclear metal cryptates at physiological pH: selectivity for fumarate vs. maleate.
Dalton Trans
PUBLISHED: 08-06-2013
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Cryptand L (L = N[(CH2)2NHCH2(2,6-C10H6)CH2NH(CH2)2]3N) and its dinuclear metal cryptates [Zn2L](NO3)4 (1) and [Cu2L](ClO4)4 (2) have been prepared, and the binding properties of the cryptates with fumarate and its cis isomer maleate were investigated using fluorescent spectra, (1)H NMR titrations and single crystal X-ray diffraction analysis for [(Cu2L)(fum)][ClO4]2 (3) (fum = fumarate). Thanks to the size and shape matching effect, the cryptates can selectively recognize fumarate at physiological pH, with an association constant almost 18-fold larger than that of maleate, forming a cradle-like cascade complex.
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Synthesis of highly branched gold nanodendrites with a narrow size distribution and tunable NIR and SERS using a multiamine surfactant.
ACS Appl Mater Interfaces
PUBLISHED: 07-17-2013
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Gold nanodendrites with a long and densely branched morphology were fabricated by a seed-mediated method in a solution containing gold nanoparticles (AuNPs), bis(amidoethyl-carbamoylethyl)octadecylamine (C18N3), HAuCl4, and the reducing agent ascorbic acid (AA). The length and density of the branches could be mediated by changing the AuNP seed and AA concentrations. The amphiphilic C18N3 molecules function as a template and induce the unique morphology of the AuNPs/C18N3 structures. The localized surface plasmon resonance (LSPR) peaks of the gold nanodendrites can be modulated from the visible (?530 nm) to the near-infrared region (?1100 nm) of the electromagnetic spectrum. Surface-enhanced Raman scattering (SERS) signals using rhodamine can also be mediated by changing the seed and AA concentrations. These unique highly branched gold nanodendrites with a narrow size distribution and tunable NIR and SERS spectra should have great potential in sensing applications.
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Three-dimensional contrast-enhanced magnetic resonance venography for detection of renal vein thrombosis: comparison with multidetector CT venography.
Acta Radiol
PUBLISHED: 06-21-2013
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Renal vein thrombosis is not uncommon, however, there have been few reports on the diagnostic accuracy of three-dimensional contrast-enhanced magnetic resonance venography (3D-CE-MRV) in the detection of renal vein thrombosis (RVT).
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A {Cr2Dy4} compressed octahedron: the first sulfate-based single-molecule magnet.
Dalton Trans
PUBLISHED: 06-19-2013
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A unique compressed octahedral {Cr(III)(2)Dy(III)(4)} aggregate is the first sulfate-based single-molecule magnet (SMM) with U(eff)/k(B) = 39.7 K and ?(0) = 2.9 × 10(-9) s. Its synthesis opens a new avenue to prepare 3d-4f coordination clusters.
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Hydrogen peroxide induces apoptosis in human dental pulp cells via caspase-9 dependent pathway.
J Endod
PUBLISHED: 05-22-2013
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Reactive oxygen species are a group of metabolic intermediates produced during oxidative metabolism in eukaryotic cells. They include superoxide anion (O2(-)), hydrogen peroxide (H2O2), hydroxyl radical (·OH), and (1)O2. Of these intermediates, H2O2 is the most stable. Dental pulp cells can be invaded by tooth bleaching, laser radiation, and dental materials. This can influence the intracellular level of reactive oxygen species. Apoptosis, which is the best-known form of programmed cell death, is pivotal to tissue development and regeneration. Little information is available regarding the relationship between H2O2 and apoptosis of human dental pulp cells (hDPCs). The purpose of this study was to investigate whether H2O2 can induce apoptosis in hDPCs and its signaling way.
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Application of "papillary-like main pancreatic duct invaginated" pancreaticojejunostomy for normal soft pancreas cases.
Sci Rep
PUBLISHED: 05-13-2013
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Pancreaticojejunostomy is the key procedure of pancreaticoduodenectomy. Our study introduced a new pancreaticojejunal (PJ) anastomosis named "papillary-like main pancreatic duct invaginated" pancreaticojejunostomy. Nighty-two patients underwent pancreaticojejunostomy with either conventional duct-to-mucosa pancreaticojejunostomy or the new "papillary-like main pancreatic duct invaginated" techniques were analyzed retrospectively from January 2010 to September 2012. The incidence of pancreatic fistula was 15.7% (8/51) for the "papillary-like main pancreatic duct invaginated" group and 19.5% (8/41) for the duct-to-mucosa fashion respectively. It is noteworthy that the rate of grade B/C postoperative pancreatic fistula (POPF) in the "papillary-like main pancreatic duct invaginated" group was significantly lower than that of the duct-to-mucosa group (P = 0.039). There were no differences in the incidence of postoperative morbidity and mortality such as postoperative hemorrhage, delayed gastric emptying or remnant pancreatitis. The "papillary-like main pancreatic duct invaginated" pancreaticojejunostomy could provide a feasible option to pancreatic surgeons for patients with normal soft pancreas.
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CA125 is Superior to CA19-9 in Predicting the Resectability of Pancreatic Cancer.
J. Gastrointest. Surg.
PUBLISHED: 04-23-2013
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Although carbohydrate antigen 19-9 (CA19-9) has been reported as a biomarker to predict the resectability of pancreatic cancer, several limitations have restricted its clinical use.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.