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Find video protocols related to scientific articles indexed in Pubmed.
Discovery and Characterization of the Tuberculosis Drug Lead Ecumicin.
Org. Lett.
PUBLISHED: 11-20-2014
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The new tuberculosis (TB) lead ecumicin (1), a cyclic tridecapeptide, was isolated from Nonomuraea sp. MJM5123, following a high-throughput campaign for anti-TB activity. The large molecular weight of 1599 amu detected by LC-HR-MS precluded the initial inference of its molecular formula. The individual building blocks were identified by extensive NMR experiments. The resulting two possible planar structures were distinguished by LC-MS(2). Determination of absolute configuration and unambiguous structural confirmation were carried out by X-ray crystallography and Marfey's analysis.
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[Nasopharyngeal carriage rate, antimicrobial resistance and serotype distribution of Streptococcus pneumoniae among children with upper respiratory infection].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 10-26-2014
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To investigate nasopharyngeal carriage rate, antimicrobial resistance and serotype distribution of Streptococcus pneumoniae among children with upper respiratory infection.
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Effect of cardiac resynchronization therapy on ventricular repolarization: A meta-analysis.
Anadolu Kardiyol Derg
PUBLISHED: 10-22-2014
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Cardiac resynchronization therapy (CRT) was thought to have a proarrhythmic effect on ventricular repolarization. But the results of previous studies were inconsistent. The aim of this study was to determine the effect of CRT on ventricular repolarization.
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Dynamic changes and clinical significance of serum tryptase levels in STEMI patients treated with primary PCI.
Biomarkers
PUBLISHED: 10-18-2014
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Abstract Objective: To investigate the dynamic changes in serum tryptase levels and their association with clinical data in patients with acute ST-elevation myocardial infarction (STEMI) treated with primary percutaneous coronary intervention (PCI).
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Multiple-network classification of childhood autism using functional connectivity dynamics.
Med Image Comput Comput Assist Interv
PUBLISHED: 10-17-2014
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Characterization of disease using stationary resting-state functional connectivity (FC) has provided important hallmarks of abnormal brain activation in many domains. Recent studies of resting-state functional magnetic resonance imaging (fMRI), however, suggest there is a considerable amount of additional knowledge to be gained by investigating the variability in FC over the course of a scan. While a few studies have begun to explore the properties of dynamic FC for characterizing disease, the analysis of dynamic FC over multiple networks at multiple time scales has yet to be fully examined. In this study, we combine dynamic connectivity features in a multi-network, multi-scale approach to evaluate the method's potential in better classifying childhood autism. Specifically, from a set of group-level intrinsic connectivity networks (ICNs), we use sliding window correlations to compute intra-network connectivity on the subject level. We derive dynamic FC features for all ICNs over a large range of window sizes and then use a multiple kernel support vector machine (MK-SVM) model to combine a subset of these features for classification. We compare the performance our multi-network, dynamic approach to the best results obtained from single-network dynamic FC features and those obtained from both single- and multi-network static FC features. Our experiments show that integrating multiple networks on different dynamic scales has a clear superiority over these existing methods.
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Water-Driven Micromotors for Rapid Photocatalytic Degradation of Biological and Chemical Warfare Agents.
ACS Nano
PUBLISHED: 10-10-2014
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Threats of chemical and biological warfare agents (CBWA) represent a serious global concern and require rapid and efficient neutralization methods. We present a highly effective micromotor strategy for photocatalytic degradation of CBWA based on light-activated TiO2/Au/Mg microspheres that propel autonomously in natural water and obviate the need for external fuel, decontaminating reagent, or mechanical agitation. The activated TiO2/Au/Mg micromotors generate highly reactive oxygen species responsible for the efficient destruction of the cell membranes of the anthrax simulant Bacillus globigii spore, as well as rapid and complete in situ mineralization of the highly persistent organophosphate nerve agents into nonharmful products. The water-driven propulsion of the TiO2/Au/Mg micromotors facilitates efficient fluid transport and dispersion of the photogenerated reactive oxidative species and their interaction with the CBWA. Coupling of the photocatalytic surface of the micromotors and their autonomous water-driven propulsion thus leads to a reagent-free operation which holds a considerable promise for diverse "green" defense and environmental applications.
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[Medicinal plant hairy roots generating and their applications].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 10-03-2014
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As a kind of the plant tissue cultures, hairy root culture is characterized by rapid growth without exogenous hormones source and high yield of secondary metabolites, which attracted the attention of scholars in resent years. This work systematically summarized the research of medicinal plant hairy roots, including the mechanism, current situation of medicinal plant hairy roots, and their applications.
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Shenqi Fuzheng Injection () for advanced gastric cancer: A systematic review of randomized controlled trials.
Chin J Integr Med
PUBLISHED: 09-23-2014
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To evaluate the effectiveness of Shenqi Fuzheng Injection (, SFI) combined with chemotherapy for advanced gastric cancer.
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[Expression of cancer stem cell marker USP22 in laryngeal squamous cell carcinoma].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-23-2014
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To investigate the expression of cancer stem cell marker USP22 in laryngeal squamous cell carcinoma (LSCC) and clinical implications.
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Characterization of eight terpenoids from tissue cultures of the Chinese herbal plant, Tripterygium wilfordii, by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry.
Biomed. Chromatogr.
PUBLISHED: 09-20-2014
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In this study, a reliable method for analysis and identification of eight terpenoids in tissue cultures of Tripterygium wilfordii has been established using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS). Our study indicated that sterile seedlings, callus cultures and cell-suspension cultures can rapidly increase the amount of biological materials. HPLC-ESI-MS was used to identify terpenoids from the extracts of these tissue cultures. Triptolide, triptophenolide, celastrol and wilforlide A were unambiguously determined by comparing the retention times, UV spectral data, and mass fragmentation behaviors with those of the reference compounds. Another four compounds were tentatively identified as triptonoterpenol, triptonoterpene, 22?-hydroxy-3-oxoolean-12-en-29-oic acid and wilforlide B, based on their UV and mass spectrometry spectra. The quantitative analysis showed that all three materials contain triptolide, triptophenolide, celastrol, wilforlide A, and the contents of the four compounds in the cell-suspension cultures were 53.1, 240, 129 and 964?µg/g, respectively, which were at least 2.0-fold higher than these in the sterile seedlings and callus cultures. Considering the known pharmacological activity of triptolide and celastrol, we recommend the cell-suspension cultures as biological materials for future studies, such as clinical and toxicological studies. The developed method was validated by the evaluation of its precision, linearity, detection limits and recovery, and it was successfully used to identify and quantify the terpenoids in the tissue cultures.
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Virulent and attenuated strains of duck hepatitis A virus elicit discordant innate immune responses in vivo.
J. Gen. Virol.
PUBLISHED: 09-12-2014
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Previous studies of duck hepatitis A virus infection have focused only on the pathogenicity and host response of one strain. Here, we show that the virulent SH strain and the attenuated FC64 strain induced varied pathogenicity, apoptosis and immune responses in the livers of 1-day-old ducklings. SH infection caused apoptosis and visible lesions in the liver; serum aspartate aminotransferase, alanine transaminase, alkaline phosphatase, ?-glutamyltransferase and total bilirubin activities were markedly upregulated; and ducklings died at 36 h post-infection (p.i.). However, FC64 infection did not induce significant symptoms or impair liver function, and all of the infected ducklings remained healthy. In addition, both virus strains replicated well in the liver, spleen and intestine, whilst the SH strain replicated more efficiently than FC64. IFN-?, IL-2, inducible nitric oxide synthase and nitric oxide were strongly induced by SH infection, and may be associated with the pathogenicity of the SH strain. IFN-?, IFN-?, IFN-stimulated transmembrane protein 1, IFN-stimulated gene 12, 2',5'-oligoadenylate synthetase-like and IL-6 were moderately induced by SH infection at 24 h p.i., and dramatically induced by FC64 infection at 36 h p.i. The intensive induction of cytokines by FC64 may be involved in restriction of virus replication and stimulation of adaptive immune responses. Ducklings inoculated with FC64 produced high levels of antiviral antibodies within 45 days p.i. The low virulence and strong immune response of FC64 rendered this strain a good vaccine candidate, as confirmed by a protective assay in this study.
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Fabrication of graphene-isolated-Au-nanocrystal nanostructures for multimodal cell imaging and photothermal-enhanced chemotherapy.
Sci Rep
PUBLISHED: 09-02-2014
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Using nanomaterials to develop multimodal systems has generated cutting-edge biomedical functions. Herein, we develop a simple chemical-vapor-deposition method to fabricate graphene-isolated-Au-nanocrystal (GIAN) nanostructures. A thin layer of graphene is precisely deposited on the surfaces of gold nanocrystals to enable unique capabilities. First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes. Second, GIANs can be used for multimodal cell imaging by both Raman scattering and near-infrared (NIR) two-photon luminescence. Third, GIANs provide a platform for loading anticancer drugs such as doxorubicin (DOX) for therapy. Finally, their NIR absorption properties give GIANs photothermal therapeutic capability in combination with chemotherapy. Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy. The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.
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Sterigmatocystin-induced oxidative DNA damage in human liver-derived cell line through lysosomal damage.
Toxicol In Vitro
PUBLISHED: 08-28-2014
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Sterigmatocystin (STC) is a carcinogenic and mutagenic mycotoxin produced by fungi of many Aspergillus species. As a precursor of the aflatoxins, STC is a risk factor of liver cancer. The objective of this study is to investigate STC-induced DNA damage and underlying mechanisms. The single cell gel electrophoresis (SCGE) assay was applied to assess DNA damage induced by STC. To clarify the underlying mechanisms, we measured the intracellular generation of reactive oxygen species (ROS) using dichlorofluorescein diacetate as a fluorochrome. The level of oxidative DNA damage was evaluated by immunocytochemical analysis of 8-hydroxydeoxyguanosine (8-OHdG) and the acridine orange (AO) was used to measure the changes of lysosomal membrane stability. A significant dose-dependent increase of DNA strand breaks was found after treatment with STC (3 and 6?M) respectively for 1h. Increases in ROS level and the expression of 8-OHdG were also observed. A statistically significant increase in AO fluorescence intensity was observed in cells treated with STC (1.5, 3 and 6?M) for 1h. The DNA strand breaks induced by STC were almost prevented in cells pretreated with NH4Cl (10mM) and NAC (10mM) for 1h. Our results thus indicated that STC exerts genotoxic effects on HepG2 cells, most likely through oxidative stress and lysosomal leakage.
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Local airway anesthesia attenuates hemodynamic responses to intubation and extubation in hypertensive surgical patients.
Med. Sci. Monit.
PUBLISHED: 08-26-2014
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The aim of this study was to evaluate the effects of topical ropivacaine anesthesia on hemodynamic responses during intubation and extubation of hypertensive patients.
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Malocclusion, orthodontic treatment, and the Oral Health Impact Profile (OHIP-14) Systematic review and meta-analysis.
Angle Orthod
PUBLISHED: 08-26-2014
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Objective: To synthesize evidence on the impact of malocclusion and its associated treatment on people's quality of life across studies that used the Oral Health Impact Profile (OHIP-14) questionnaire in the adult population. Materials and Methods: A systematic search of the English literature using Medline, PubMed, and EMBASE yielded 98 unique citations. Studies using OHIP-14 with individuals 15 years of age and older were included. After initial screening, 64 citations were excluded and another 9 were excluded after reading full text reports; the remaining 25 were included in the review. All studies were observational and used one of three study designs: 11 compared the same group before and after treatment (pre-post design), 10 compared groups with and without malocclusion (independent groups design) and four compared an orthodontically treated group with an independent group requiring treatment (treated-untreated groups design). Only three studies using the pre-post design and four using the independent groups design reported comparable OHIP-14 data and were combined in separate meta-analyses. Meta-analysis was carried out using metan command in Stata. Results: The standardized mean difference (SMD) in OHIP-14 total score was 1.29 (95% CI: 0.67 to 1.92) for the three studies using the pre-post design. Similarly, the SMD score was 0.84 (95% CI: 0.25 to 1.43) for the four studies using the independent groups design. There was evidence of high heterogeneity and publication bias among the studies included. Conclusions: This meta-analysis revealed that OHIP-14 scores were significantly lower after receiving treatment for malocclusion and in individuals without malocclusion/orthodontic treatment need compared to those with such condition (independent groups).
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Redox mediated angiogenesis in the hypoxic joint of inflammatory arthritis.
PUBLISHED: 08-22-2014
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Objective: Inflammatory arthritis (IA) is associated with joint inflammation, synovial tissue proliferation and degradation of articular cartilage and bone. Angiogenesis is an early and fundamental component of synovial inflammation. Oxygen metabolism is recognized as an important mediator of joint vascular remodeling. In this study we investigate if in-vivo synovial hypoxia (tpO2 ) and TNF blocking therapy (TNFi) alters synovial vascular expression of Nicotinamide Adenine Dinucleotide Phosphate Oxidase (Nox2), and how this regulates angiogenic mechanisms. Methods: Nox2 protein/mRNA expression was examined in IA patients pre/post-TNFi and in a mouse model of collagen-induced arthritis (CIA). Pro-angiogenic processes were assessed in Human Microvascular Endothelial Cells (HMVECs) following culture with Nox2 activators (TNF-? and 4-HNE), Nox siRNA and inhibitor (DPI) under normoxia or 3% hypoxia. Results: We demonstrated significantly increased Nox2 expression in IA and CIA joints compared to controls. Nox2 expression was higher in patients with synovial tpO2 <3% compared to those with tpO2 >3% (p<0.05), and correlated with in-vivo macroscopic/microscopic scores of angiogenesis, such as vascularity, VEGF, Ang2, Factor VIII, NCAM and ?-SMA (all p<0.05). Decrease in Nox2 expression was paralleled by an increase in in-vivo tpO2 only in those patients who were defined as TNFi responders. In-vitro Nox2 activators and 3% hypoxia significantly promoted HMVEC migration, angiogenic tube-formation and secretion of pro-angiogenic mediators, effects that were blocked by Nox2 siRNA or the Nox2 inhibitor DPI. Conclusion: We demonstrated that hypoxia activates expression of Nox2 protein and Nox2-induced oxidative stress may be an initiating factor in driving angiogenesis. © 2014 American College of Rheumatology.
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Intersubject variability of and genetic effects on the brain's functional connectivity during infancy.
J. Neurosci.
PUBLISHED: 08-22-2014
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Infancy is a period featuring a high level of intersubject variability but the brain basis for such variability and the potential genetic/environmental contributions remain largely unexplored. The assessment of the brain's functional connectivity during infancy by the resting state functional magnetic resonance imaging (rsfMRI) technique (Biswal et al., 1995) provides a unique means to probe the brain basis of intersubject variability during infancy. In this study, an unusually large typically developing human infant sample including 58 singletons, 132 dizygotic twins, and 98 monozygotic twins with rsfMRI scans during the first 2 years of life was recruited to delineate the spatial and temporal developmental patterns of both the intersubject variability of and genetic effects on the brain's functional connectivity. Through systematic voxelwise functional connectivity analyses, our results revealed that the intersubject variability at birth features lower variability in primary functional areas but higher values in association areas. Although the relative pattern remains largely consistent, the magnitude of intersubject variability undergoes an interesting U-shaped growth during the first 2 years of life. Overall, the intersubject variability patterns during infancy show both adult-like and infant-specific characteristics (Mueller et al., 2013). On the other hand, age-dependent genetic effects were observed showing significant but bidirectional relationships with intersubject variability. The temporal and spatial patterns of the intersubject variability of and genetic contributions to the brain's functional connectivity documented in this study shed light on the largely uncharted functional development of the brain during infancy.
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[Effects of mild hypothermia plus ifenprodil on apoptosis inducing factor translocation after global cerebral ischemia-reperfusion in rats].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 08-22-2014
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To explore the effects of mild hypothermia combined with ifenprodil on the survival of neuronal and translocation of apoptosis inducing factor (AIF) following global cerebral ischemia-reperfusion to understand the mechanism of combination in cerebral resuscitation.
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Role of poly(ADP-ribose) glycohydrolase silencing in DNA hypomethylation induced by benzo(a)pyrene.
Biochem. Biophys. Res. Commun.
PUBLISHED: 08-20-2014
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Benzo(a)pyrene (BaP) is a known carcinogen cytotoxic which can trigger extensive cellular responses. Many evidences suggest that inhibitors of poly(ADP-ribose) glycohydrolase (PARG) are potent anticancer drug candidates. However, the role of PARG in BaP carcinogenesis is less understood. Here we used PARG-deficient human bronchial epithelial cell line (shPARG cell) as an in vitro model, and investigated the role of PARG silencing in DNA methylation pattern changed by BaP. Our study shows, BaP treatment decreased global DNA methylation levels in 16HBE cells in a dose-dependent manner, but no dramatic changes were observed in shPARG cells. Further investigation revealed PARG silencing protected DNA methyltransferases (DNMTs) activity from change by BaP exposure. Interestingly, Dnmt1 is PARylated in PARG-null cells after BaP exposure. The results show a role for PARG silencing in DNA hypomethylation induced by BaP that may provide new clue for cancer therapy.
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Systematic evaluation of percutaneous radiofrequency ablation versus percutaneous ethanol injection for the treatment of small hepatocellular carcinoma: a meta-analysis.
Eur. J. Med. Res.
PUBLISHED: 08-20-2014
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Radiofrequency ablation (RFA) and percutaneous ethanol injection (PEI) have been used for patients with hepatocellular carcinoma (HCC). However, which therapy is superior remains to be further elucidated. We aimed to conduct a systematic review to assess survival and local tumor recurrence rate with RFA compared with PEI therapy for HCC.
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Mesenchymal stem cells overexpressing integrin-linked kinase attenuate left ventricular remodeling and improve cardiac function after myocardial infarction.
Mol. Cell. Biochem.
PUBLISHED: 08-19-2014
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In the present study, we investigated whether mesenchymal stem cells (MSCs) overexpressing integrin-linked kinase (ILK) might regulate ventricular remodeling and cardiac function in a porcine myocardial infarction model. ILK-modified MSCs (ILK-MSCs) (n = 8), MSCs (n = 8) or placebo (n = 8) were injected into peri-infarct myocardium 7 days after ligation of the left anterior descending coronary artery. ILK expression was confirmed by immunofluorescence, real-time PCR, Western blot analysis, and flow cytometry. In vitro assays indicated increased proliferation and reduced apoptosis of MSCs due to overexpression of ILK. Echocardiographic, single-photon emission computed tomography and positron emission tomography analyses demonstrated preserved cardiac function and myocardial perfusion. Reduced fibrosis, increased cardiomyocyte proliferation, and enhanced angiogenesis were observed in the ILK-MSC group. Reduced apoptosis, as demonstrated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis, was also noted. In conclusion, ILK promotes MSC proliferation and suppresses apoptosis. ILK-MSC transplantation improves ventricular remodeling and cardiac function in pigs after MI. It is associated with increased angiogenesis, reduced apoptosis, and increased cardiomyocyte proliferation. This may represent a new approach to the treatment of post-infarct remodeling and subsequent heart failure.
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[Analysis of the curative effect of ABO-incompatible liver transplantation in the treatment in patients with acute severe liver disease].
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
PUBLISHED: 08-16-2014
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To analyze and evaluate the clinical effect of ABO-incompatible liver transplantation in the treatment of acute severe liver disease.
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Synthetic micro/nanomotors in drug delivery.
Nanoscale
PUBLISHED: 08-06-2014
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Nanomachines offer considerable promise for the treatment of diseases. The ability of man-made nanomotors to rapidly deliver therapeutic payloads to their target destination represents a novel nanomedicine approach. Synthetic nanomotors, based on a multitude of propulsion mechanisms, have been developed over the past decade toward diverse biomedical applications. In this review article, we journey from the use of chemically powered drug-delivery nanovehicles to externally actuated (fuel-free) drug-delivery nanomachine platforms, and conclude with future prospects and challenges for such practical propelling drug-delivery systems. As future micro/nanomachines become more powerful and functional, these tiny devices are expected to perform more demanding biomedical tasks and benefit different drug delivery applications.
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Interactions between E-cadherin and microRNA deregulation in head and neck cancers: the potential interplay.
Biomed Res Int
PUBLISHED: 08-04-2014
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E-cadherin expression in the head and neck epithelium is essential for the morphogenesis and homeostasis of epithelial tissues. The cadherin-mediated cell-cell contacts are required for the anchorage-dependent growth of epithelial cells. Further, survival and proliferation require physical tethering created by proper cell-cell adhesion. Otherwise, the squamous epithelial cells will undergo programmed cell death. Head and neck cancers can escape from anoikis and enter into the epithelial-mesenchymal transition stages via the modulation of E-cadherin expression with epigenetic mechanisms. At epigenetic level, gene expression control is not dependent on the DNA sequence. In the context of E-cadherin regulation in head and neck cancers, 2 major mechanisms including de novo promoter hypermethylation and microRNA dysregulation are most extensively studied. Both of them control E-cadherin expression at transcription level and subsequently hinder the overall E-cadherin protein level in the head and neck cancer cells. Increasing evidence suggested that microRNA mediated E-cadherin expression in the head and neck cancers by directly/indirectly targeting the transcription suppressors of E-cadherin, ZEB1 and ZEB2.
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Self-Propelled Activated Carbon Janus Micromotors for Efficient Water Purification.
Small
PUBLISHED: 07-24-2014
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Self-propelled activated carbon-based Janus particle micromotors that display efficient locomotion in environmental matrices and offer effective 'on-the-fly' removal of wide range of organic and inorganic pollutants are described. The new bubble-propelled activated carbon Janus micromotors rely on the asymmetric deposition of a catalytic Pt patch on the surface of activated carbon microspheres. The rough surface of the activated carbon microsphere substrate results in a microporous Pt structure to provide a highly catalytic layer, which leads to an effective bubble evolution and propulsion at remarkable speeds of over 500 ?m/s. Such coupling of the high adsorption capacity of carbon nanoadsorbents with the rapid movement of these catalytic Janus micromotors, along with the corresponding fluid dynamics and mixing, results in a highly efficient moving adsorption platform and a greatly accelerated water purification. The adsorption kinetics and adsorption isotherms have been investigated. The remarkable decontamination efficiency of self-propelled activated carbon-based Janus micromotors is illustrated towards the rapid removal of heavy metals, nitroaromatic explosives, organophosphorous nerve agents and azo-dye compounds, indicating considerable promise for diverse environmental, defense, and public health applications.
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Association between peak neutrophil count, clopidogrel loading dose, and left ventricular systolic function in patients with primary percutaneous coronary intervention.
Mediators Inflamm.
PUBLISHED: 07-24-2014
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Inflammation plays an important role in plaque development and left ventricular remodeling during acute myocardial infarction (AMI). Clopidogrel may exhibit some anti-inflammatory properties and high loading dose of clopidogrel results in improved clinical outcomes in patients with AMI. 357 patients who received successful primary percutaneous coronary intervention from January 2008 to March 2011 in Peking University Third Hospital were included in this study. Different loading dose of clopidogrel (300?mg, 450?mg, or 600?mg) was given at the discretion of the clinician. Neutrophils reached their peak values on the first day after AMI. Higher levels of peak neutrophil and lower left ventricular ejection fraction (LVEF) were found in patients of low clopidogrel loading dose group (300?mg or 450?mg). After adjusting for the related confounders, a logistic regression model showed that low clopidogrel loading dose remained an independent predictor of low LVEF (LVEF ? 50%) [OR: 1.97, 95% CI: 1.03-3.79, P = 0.04]. Low clopidogrel loading dose was associated with higher peak neutrophil count and poor left ventricular systolic function, suggesting an important role of clopidogrel loading dose in the improvement of left ventricular function and high loading dose may exhibit better anti-inflammatory properties.
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Genetic and molecular predictors of high vancomycin MIC in Staphylococcus aureus bacteremia isolates.
J. Clin. Microbiol.
PUBLISHED: 07-16-2014
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An elevated vancomycin MIC is associated with poor outcomes in Staphylococcus aureus bacteremia (SAB) and is reported in patients with methicillin-susceptible S. aureus (MSSA) bacteremia in the absence of vancomycin treatment. Here, using DNA microarray and phenotype analysis, we investigated the genetic predictors and accessory gene regulator (agr) function and their relationship with elevated vancomycin MIC using blood culture isolates from a multicenter binational cohort of patients with SAB. Specific clonal complexes were associated with elevated (clonal complex 8 [CC8] [P < 0.001]) or low (CC22 [P < 0.001], CC88 [P < 0.001], and CC188 [P = 0.002]) vancomycin MIC. agr dysfunction (P = 0.014) or agr genotype II (P = 0.043) were also associated with an elevated vancomycin MIC. Specific resistance and virulence genes were also linked to an elevated vancomycin MIC, including blaZ (P = 0.002), sea (P < 0.001), clfA (P < 0.001), splA (P = 0.001), and the arginine catabolic mobile element (ACME) locus (P = 0.02). These data suggest that inherent organism characteristics may explain the link between elevated vancomycin MICs and poor outcomes in patients with SAB, regardless of the antibiotic treatment received. A consideration of clonal specificity should be included in future research when attempting to ascertain treatment effects or clinical outcomes.
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[Expression and significance of PTEN and HIF-1? proteins in sinonasal inverted papilloma].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 07-15-2014
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To investigate the expression and clinical significance of phosphatase and tensin homology deleted on ehromosome ten (PTEN) and hypoxia-inducible factor 1 alpha (HIF-1?) in sinonasal inverted papilloma (SNIP) of different pathological grades.
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Analysis of urinary methylated nucleosides of patients with coronary artery disease by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry.
Rapid Commun. Mass Spectrom.
PUBLISHED: 07-13-2014
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In recent years, methylated nucleosides have been considered to be potential biomarkers to human diseases. The early diagnosis of coronary artery disease (CAD) is an unsolved problem in clinical cardiology. The aim of our study is to evaluate whether urinary methylated nucleosides can serve as useful biomarkers for CAD.
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Effects of hypercapnia on T cells in lung ischemia/reperfusion injury after lung transplantation.
Exp. Biol. Med. (Maywood)
PUBLISHED: 07-10-2014
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T cells play a key role in lung ischemia/reperfusion injury (IRI). Hypercapnia has been indicated to decrease IRI and inhibit immunity. This study aimed to evaluate the effects of hypercapnia on T cells during lung IRI and to identify the underlying mechanism of these effects. In the in vivo study, rat recipients of lung transplants were randomized into a control group M and a hypercapnia group H. Peripheral blood T cells and cytokines were analyzed during reperfusion. In the in vitro study, we analyzed the T cells and cytokine levels in culture media from phytohemagglutinin-stimulated T cells from normal rats, stimulated under the normal (group C), hypercapnic (group H), or buffer hypercapnic (group BH) condition. In the in vivo study, the CD3(+)/CD4(+) T-cell ratio and interleukin (IL)-2, IL-8, interferon (IFN)-?, intracellular adhesion molecule (ICAM)-1, and P-selectin levels were decreased, but the IL-4 and IL-10 levels were increased, after reperfusion in group H compared to group M. In the in vitro study, groups H and BH exhibited a decreased CD2(+)/CD28(+) ratio and IL-2 and IFN-? levels, but elevated IL-4 and IL-10 levels, compared to group C. The CD2(+)/CD28(+) ratio was not different between groups BH and H; however, group H evidenced a lower IL-2 level and higher IL-4 and IL-10 levels compared to group BH. Hypercapnia decreased the CD3(+)/CD4(+) T-cell ratio and pro-inflammatory cytokine levels, but promoted anti-inflammatory factors in lung IRI. Hypercapnia inhibits CD2 and CD28 in T cells by CO2 and modulates T-cell cytokines via acidosis.
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Toughness condition for a graph to be a fractional (g, f, n)-critical deleted graph.
ScientificWorldJournal
PUBLISHED: 07-09-2014
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A graph G is called a fractional (g, f)-deleted graph if G - {e} admits a fractional (g, f)-factor for any e ? E(G). A graph G is called a fractional (g, f, n)-critical deleted graph if, after deleting any n vertices from G, the resulting graph is still a fractional (g, f)-deleted graph. The toughness, as the parameter for measuring the vulnerability of communication networks, has received significant attention in computer science. In this paper, we present the relationship between toughness and fractional (g, f, n)-critical deleted graphs. It is determined that G is fractional (g, f, n)-critical deleted if t(G) ? ((b (2) - 1 + bn)/a).
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Development of thalamocortical connectivity during infancy and its cognitive correlations.
J. Neurosci.
PUBLISHED: 07-04-2014
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Although commonly viewed as a sensory information relay center, the thalamus has been increasingly recognized as an essential node in various higher-order cognitive circuits, and the underlying thalamocortical interaction mechanism has attracted increasing scientific interest. However, the development of thalamocortical connections and how such development relates to cognitive processes during the earliest stages of life remain largely unknown. Leveraging a large human pediatric sample (N = 143) with longitudinal resting-state fMRI scans and cognitive data collected during the first 2 years of life, we aimed to characterize the age-dependent development of thalamocortical connectivity patterns by examining the functional relationship between the thalamus and nine cortical functional networks and determine the correlation between thalamocortical connectivity and cognitive performance at ages 1 and 2 years. Our results revealed that the thalamus-sensorimotor and thalamus-salience connectivity networks were already present in neonates, whereas the thalamus-medial visual and thalamus-default mode network connectivity emerged later, at 1 year of age. More importantly, brain-behavior analyses based on the Mullen Early Learning Composite Score and visual-spatial working memory performance measured at 1 and 2 years of age highlighted significant correlations with the thalamus-salience network connectivity. These results provide new insights into the understudied early functional brain development process and shed light on the behavioral importance of the emerging thalamocortical connectivity during infancy.
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Ubiquitin?specific protease 22?induced autophagy is correlated with poor prognosis of pancreatic cancer.
Oncol. Rep.
PUBLISHED: 06-26-2014
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Ubiquitin?specific protease 22 (USP22) is a component of the transcription regulatory histone acetylation complex SAGA, which broadly regulates gene transcription and correlates with cancer progression, metastasis and prognosis. Autophagy is a cell pathway with dual functions that promotes cell survival or death. However, it is not known whether USP22 can regulate autophagy in pancreatic cancer. In the present study, we first identified that USP22 was overexpressed in a large number of pancreatic cancer patient samples, concomitant with the increased expression of LC3, a marker of autophagy. Statistical analysis revealed that the increase in USP22 and autophagy was positively correlated with poor prognosis of pancreatic cancer patients. Further investigation using a human pancreatic cancer cell (Panc?1) identified that the overexpression of USP22 increased the processing of LC3 into the active form LC3?II and the number of autophagosomes, thus leading to enhanced autophagy. Activation of ERK1/2 kinase rather than AKT1 by USP22 was found to be one of the mechanisms promoting LC3 processing. USP22?induced autophagy was also found to enhance cell proliferation and resistance to starvation and chemotherapeutic drugs in Panc?1 cells, therefore expressing an overall effect that promotes cell survival. Collectively, the present study demonstrated a new function of USP22 that induces autophagy, thus leading to the poor prognosis of pancreatic cancer.
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Nanomotor lithography.
Nat Commun
PUBLISHED: 06-20-2014
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The rapid miniaturization of devices and machines has fuelled the evolution of advanced fabrication techniques. However, the complexity and high cost of the state-of-the-art high-resolution lithographic systems are prompting unconventional routes for nanoscale patterning. Inspired by natural nanomachines, synthetic nanomotors have recently demonstrated remarkable performance and functionality. Here we report a new nano-patterning approach, named 'nanomotor lithography', which translates the autonomous movement trajectories of nanomotors into controlled surface features. As a proof of principle, we use metallic nanowire motors as mobile nanomasks and Janus sphere motors as near-field nanolenses to manipulate light beams for generating a myriad of nanoscale features through modular nanomotor design. The complex spatially defined nanofeatures using these dynamic nanoscale optical elements can be achieved through organized assembly and remote guidance of multiple nanomotors. Such ability to transform predetermined paths of moving nanomachines to defined surface patterns provides a unique nanofabrication platform for creating diverse nanodevices.
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Expression of DNA topoisomerase II-?: Clinical significance in laryngeal carcinoma.
Oncol Lett
PUBLISHED: 06-19-2014
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DNA topoisomerase II-? (Topo II-?) is essential for numerous cell processes, including DNA replication, transcription, recombination, and chromosome separation and condensation. Altered Topo II-? expression may lead to carcinogenesis and cancer progression. The aim of the present study was to investigate the association between Topo II-? expression levels and clinicopathological data from laryngeal cancer patients. Immunohistochemistry was used to analyze Topo II-? expression in laryngeal squamous cell carcinoma and distant healthy tissues obtained from 70 patients. In addition, fluorescence in situ hybridization was used to detect Topo II-? amplification and chromosome 17 ploidy using a laryngeal cancer tissue microarray. The expression of Topo II-? protein was detected in 71.43% (50/70) of laryngeal carcinoma tissues, in contrast to 9% of healthy tissues (2/22). Furthermore, the expression of Topo II-? protein was found to be associated with tumor de-differentiation and advanced tumor T stage. However, the expression of Topo II-? protein was not identified to be associated with Topo II-? amplification in laryngeal carcinoma, although was found to positively correlate with chromosome 17 aneuploidy (P<0.05). A higher aneuploidy rate contributed to increased expression levels of Topo II-? protein. Aberrant Topo II-? expression and chromosome 17 aneuploidy contributed to the development and progression of laryngeal cancer, indicating that targeting Topo II-? may provide a treatment strategy for patients with laryngeal cancer.
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The association between the APE1 Asp148Glu polymorphism and prostate cancer susceptibility: a meta-analysis based on case-control studies.
Mol. Genet. Genomics
PUBLISHED: 06-10-2014
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The apurinic/apyrimidinic endonuclease 1 (APE1) plays important roles in the repair of DNA damage and adducts. However, previous case-control studies on the association between the APE1 Asp148Glu polymorphism and prostate cancer susceptibility have shown contradictory results, this meta-analysis was performed to draw a more precise estimation of the relationship. A total of seven case-control studies including 1,294 cases and 1,762 controls were included for analysis. In overall, no significant associations were found in all genetic models (GG vs. TT: OR = 1.16, 95 % CI 0.89-1.52; TG vs. TT: OR = 1.04, 95 % CI 0.81-1.35; the dominant model GG + TG vs. TT: OR = 1.12, 95 % CI 0.96-1.30; the recessive model GG vs. TG + TT: OR = 0.90, 95 % CI 0.77-1.04); in the subgroup by source of control, we found a significant association for the dominant model in Hospital-based subgroup (OR = 1.34, 95 % CI 1.08-1.68), no significant associations were found in other models in the subgroups. This meta-analysis suggested that the APE1 Asp148Glu polymorphism was a risk factor for prostate cancer susceptibility in Hospital-based population.
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Thiol-based redox proteomics in cancer research.
Proteomics
PUBLISHED: 04-25-2014
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Cancer cells maintain their intracellular reactive oxygen species (ROS) concentrations at required levels for their survival. Changes in ROS concentrations can regulate biochemical signaling mechanisms that control cell function. It has been demonstrated that ROS regulate the cellular events through redox regulation of redox-sensitive proteins (redox sensors). Upon oxidative stress, redox sensors undergo redox modifications that cause the allosteric changes of these proteins and endows them with different functions. Understanding the altered functions of redox sensors and the underlying mechanisms is critical for the development of novel cancer therapeutics. Recently, a series of high throughput proteomics approaches have been developed for screening redox processes. In this manuscript, we review these methodologies and discuss the important redox sensors recently identified that are related to cancer. This article is protected by copyright. All rights reserved.
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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis.
BMC Pediatr
PUBLISHED: 04-24-2014
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Bacterial meningitis is more common in the neonatal period than any other time in life; however, it is still a challenge for the evidence based diagnosis. Strategy for identification of neonatal bacterial meningitis pathogens is presented by evaluating three different available methods to establish evidence-based diagnosis for neonatal bacterial meningitis.
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Long non-coding RNA deregulation in tongue squamous cell carcinoma.
Biomed Res Int
PUBLISHED: 04-24-2014
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The deregulated tumorigenic long non-coding RNA (lncRNA) has been reported in several malignancies. However, there is still no comprehensive study on tongue squamous cell carcinoma (SCC).
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Improved artificial bee colony algorithm based gravity matching navigation method.
Sensors (Basel)
PUBLISHED: 03-25-2014
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Gravity matching navigation algorithm is one of the key technologies for gravity aided inertial navigation systems. With the development of intelligent algorithms, the powerful search ability of the Artificial Bee Colony (ABC) algorithm makes it possible to be applied to the gravity matching navigation field. However, existing search mechanisms of basic ABC algorithms cannot meet the need for high accuracy in gravity aided navigation. Firstly, proper modifications are proposed to improve the performance of the basic ABC algorithm. Secondly, a new search mechanism is presented in this paper which is based on an improved ABC algorithm using external speed information. At last, modified Hausdorff distance is introduced to screen the possible matching results. Both simulations and ocean experiments verify the feasibility of the method, and results show that the matching rate of the method is high enough to obtain a precise matching position.
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Effects of long-term averaging of quantitative blood pressure traits on the detection of genetic associations.
Am. J. Hum. Genet.
PUBLISHED: 03-13-2014
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Blood pressure (BP) is a heritable, quantitative trait with intraindividual variability and susceptibility to measurement error. Genetic studies of BP generally use single-visit measurements and thus cannot remove variability occurring over months or years. We leveraged the idea that averaging BP measured across time would improve phenotypic accuracy and thereby increase statistical power to detect genetic associations. We studied systolic BP (SBP), diastolic BP (DBP), mean arterial pressure (MAP), and pulse pressure (PP) averaged over multiple years in 46,629 individuals of European ancestry. We identified 39 trait-variant associations across 19 independent loci (p < 5 × 10(-8)); five associations (in four loci) uniquely identified by our LTA analyses included those of SBP and MAP at 2p23 (rs1275988, near KCNK3), DBP at 2q11.2 (rs7599598, in FER1L5), and PP at 6p21 (rs10948071, near CRIP3) and 7p13 (rs2949837, near IGFBP3). Replication analyses conducted in cohorts with single-visit BP data showed positive replication of associations and a nominal association (p < 0.05). We estimated a 20% gain in statistical power with long-term average (LTA) as compared to single-visit BP association studies. Using LTA analysis, we identified genetic loci influencing BP. LTA might be one way of increasing the power of genetic associations for continuous traits in extant samples for other phenotypes that are measured serially over time.
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Benign giant-cell tumor of the common bile duct: A case report.
World J. Gastroenterol.
PUBLISHED: 03-05-2014
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Primary giant-cell tumors rarely arise in the common bile duct. We herein report a case of primary giant-cell tumor of the common bile duct. The patient was an 81-year-old male who was diagnosed with a well-defined 1.2-cm mass projecting into the lumen of the middle common bile duct. Excision of the gallbladder and extrahepatic bile duct and a Roux-en-Y cholangiojejunostomy were performed. Histologically, the tumor had no association with carcinomas of epithelial origin and was similar to giant-cell tumors of the bone. The tumor consisted of a mixture of mononuclear and multinucleated osteoclast-like giant cells. The mononuclear cells showed no atypical features, and their nuclei were similar to those of the multinucleated giant cells. CD68 was expressed on the mononuclear and multinucleated osteoclast-like giant cells, whereas CD163 immunoreactivity was restricted to the mononuclear cells. Six months after the operation, the patient was still alive and had no recurrence. The interest of this case lies in the rarity of this entity, the difficulty of preoperative diagnosis, and this tumor's possible confusion with other malignant tumors.
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Transcription regulation of E-cadherin by zinc finger E-box binding homeobox proteins in solid tumors.
Biomed Res Int
PUBLISHED: 02-28-2014
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Downregulation of E-cadherin in solid tumors with regional migration and systematic metastasis is well recognized. In view of its significance in tumorigenesis and solid cancer progression, studies on the regulatory mechanisms are important for the development of target treatment and prediction of clinical behavior for cancer patients. The vertebrate zinc finger E-box binding homeobox (ZEB) protein family comprises 2 major members: ZEB1 and ZEB2. Both contain the motif for specific binding to multiple enhancer boxes (E-boxes) located within the short-range transcription regulatory regions of the E-cadherin gene. Binding of ZEB1 and ZEB2 to the spaced E-cadherin E-boxes has been implicated in the regulation of E-cadherin expression in multiple human cancers. The widespread functions of ZEB proteins in human malignancies indicate their significance. Given the significance of E-cadherin in the solid tumors, a deeper understanding of the functional role of ZEB proteins in solid tumors could provide insights in the design of target therapy against the migratory nature of solid cancers.
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Synthesis and anti-cancer cell activity of pseudo-ginsenoside Rh2.
Steroids
PUBLISHED: 02-23-2014
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?-d-Glucopyranoside,(3?,12?,20E)-12,25-dihydroxydammar-20(22)-en-3-yl (pseudo-ginsenoside Rh2) and its 20Z-isomer were synthesized from ginsenoside Rh2 under a mild condition, via a simple three-step called acetylation, elimination-addition and saponification. In addition, their activities were evaluated by eight different human tumor cells, compared with ginsenoside Rh2 group. Results indicated that the reaction in the side chain might greatly enhance the anti-proliferative activity of ginsenosides.
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Use of Fluorescence Imaging in Combination with Patent Blue Dye versus Patent Blue Dye Alone in Sentinel Lymph Node Biopsy in Breast Cancer.
J Breast Cancer
PUBLISHED: 02-19-2014
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Near-infrared fluorescence imaging with indocyanine green (ICG) has the potential to improve sentinel lymph node (SLN) mapping in breast cancer. In this clinical trial, we compared the potential value of ICG combined with blue dye with that of blue dye alone for detecting SLNs.
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Highly sensitive nonenzymatic glucose and H2O2 sensor based on Ni(OH)2/electroreduced graphene oxide--multiwalled carbon nanotube film modified glass carbon electrode.
Talanta
PUBLISHED: 01-29-2014
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In this article, a nonenzymatic sensor based on Ni(OH)2/electroreduced graphene oxide (ERGO)-multiwalled carbon nanotube (MWNT) nanocomposites is fabricated via convenient electrodeposition of Ni(OH)2 nanoparticles on ERGO-MWNT film modified glass carbon electrode (GCE). Graphene oxide (GO) sheets can serve as surfactants to stabilize the dispersion of pristine MWNTs in aqueous solution, rendering a fine coverage of ERGO-MWNT film on GCE during the fabrication process. MWNTs perform as conducting bridges between ERGO sheets to enhance the electron transfer rate in the substrate. By combining the advantages of ERGO and MWNTs, together with electrocatalytic effect of Ni(OH)2 nanoparticles, the well-designed nanocomposites exhibit excellent sensing behavior towards glucose and hydrogen peroxide (H2O2). The linear detection ranges for glucose and H2O2 are 10-1500 µM and 10 µM-9050 µM while the detection limits are 2.7 µM and 4.0 µM, respectively. Furthermore, a very high sensitivity is achieved with 2042 µAm M(-1) cm(-2) estimated for glucose and 711 µAm M(-1) cm(-2) for H2O2. These results suggest that Ni(OH)2/ERGO-MWNT nanocomposites thus easily prepared through a green electrochemical method are promising electrode materials for biosensing. Additionally, good recoveries of analytes in real samples like urine and milk confirm the reliability of the prepared sensor in practical applications.
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Evaluation of the benefit of using blue dye in addition to indocyanine green fluorescence for sentinel lymph node biopsy in patients with breast cancer.
World J Surg Oncol
PUBLISHED: 01-26-2014
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Near infrared-guided indocyanine green (ICG) fluorescence has vast potential for guiding sentinel lymph node biopsy (SLNB) in patients with breast cancer. The purpose of this study was to evaluate any additional clinical benefit for SLNB when blue dye is used in combination with ICG.
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Risk factors for de novo hepatitis B infection in pediatric living donor liver transplantation.
World J. Gastroenterol.
PUBLISHED: 01-23-2014
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To investigate the incidence of de novo hepatitis B virus (HBV) infection after pediatric living donor liver transplantation (LDLT) and to analyze the risk factors associated with this de novo HBV infection.
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Prognostic value of coronary artery calcium score in patients with stable angina pectoris after percutaneous coronary intervention.
J Geriatr Cardiol
PUBLISHED: 01-19-2014
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To evaluate the prognostic value of the coronary artery calcium (CAC) score in patients with stable angina pectoris (SAP) who underwent percutaneous coronary intervention (PCI).
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Protective effect of omeprazole on gastric mucosal of cirrhotic portal hypertension rats.
Asian Pac J Trop Med
PUBLISHED: 01-15-2014
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To observe the protective effect of omeprazole on gastric mucosal of cirrhotic portal hypertension rats.
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Effects of atorvastatin loading prior to primary percutaneous coronary intervention on endothelial function and inflammatory factors in patients with ST-segment elevation myocardial infarction.
Exp Ther Med
PUBLISHED: 01-08-2014
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Previous studies have demonstrated the beneficial effect of statin loading prior to elective and early percutaneous coronary intervention (PCI), in which the 'pleiotropic effects' of statins may contribute to these clinical benefits. The aim of the present study was to examine the potential effects of atorvastatin loading prior to primary PCI on coronary endothelial function and inflammatory factors in patients with acute ST-segment elevation myocardial infarction (STEMI). A total of 60 patients with STEMI were randomized into three groups: Loading dose (80 mg atorvastatin prior to PCI; n=20), regular dose (20 mg atorvastatin prior to PCI; n=20) and control (without atorvastatin prior to PCI; n=20). The plasma samples were collected prior to, and immediately, 6 and 24 h after PCI in all the patients. The plasma concentrations of endothelial nitric oxide synthase (eNOS), nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-? (TNF-?) and intercellular adhesion molecule-1 (ICAM-1) were examined using ELISA. The plasma eNOS levels immediately and 24 h after PCI were significantly higher in the regular dose group compared with the other groups. However, there were no significant differences in the plasma eNOS concentration prior to and 6 h after PCI, or in the plasma NO concentration at any of the time-points among the three groups. The plasma IL-6 levels prior to PCI were significantly lower in the loading dose group compared with the other groups; however, there were no significant differences in the plasma concentration of IL-6 following PCI or in the concentrations of TNF-? and ICAM-1 at any of the time-points among the three groups. In conclusion, atorvastatin loading in patients with STEMI undergoing primary PCI may not have protective effects on endothelial function and the inflammatory reaction.
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Characterization of Lung Function Impairment in Adults with Bronchiectasis.
PLoS ONE
PUBLISHED: 01-01-2014
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Characteristics of lung function impairment in bronchiectasis is not fully understood.
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Ethanol Negatively Regulates Hepatic Differentiation of hESC by Inhibition of the MAPK/ERK Signaling Pathway In Vitro.
PLoS ONE
PUBLISHED: 01-01-2014
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Alcohol insult triggers complex events in the liver, promoting fibrogenic/inflammatory signals and in more advanced cases, aberrant matrix deposition. It is well accepted that the regenerative capacity of the adult liver is impaired during alcohol injury. The liver progenitor/stem cells have been shown to play an important role in liver regeneration -in response to various chronic injuries; however, the effects of alcohol on stem cell differentiation in the liver are not well understood.
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A case report on nephrotic syndrome associated with ankylosing spondylitis effectively treated with infliximab.
Int J Clin Exp Med
PUBLISHED: 01-01-2014
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Renal abnormalities have been reported to occur in patients with ankylosing spondylitis (AS). This study examined a patient who suffered from AS for two years. The 24-hour urine protein of the patient was 4.80 g/day. The result of renal biopsy showed minimal change. The patient was treated with infliximab. After two months, nephrotic syndrome was treated successfully.
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Development and validation of the pre-hospital stroke symptoms coping test.
PLoS ONE
PUBLISHED: 01-01-2014
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Measures of specific knowledge of coping with pre-hospital stroke symptoms can help educate high-risk patients and family caregivers. This study aimed to develop and validate the Pre-hospital Stroke Symptoms Coping Test (PSSCT).
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Multivariate longitudinal shape analysis of human lateral ventricles during the first twenty-four months of life.
PLoS ONE
PUBLISHED: 01-01-2014
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Little is known about the temporospatial shape characteristics of human lateral ventricles (LVs) during the first two years of life. This study aimed to delineate the morphological growth characteristics of LVs during early infancy using longitudinally acquired MR images in normal healthy infants.
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The co-delivery of a low-dose P-glycoprotein inhibitor with doxorubicin sterically stabilized liposomes against breast cancer with low P-glycoprotein expression.
Int J Nanomedicine
PUBLISHED: 01-01-2014
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P-glycoprotein (P-gp) inhibitors are usually used to treat tumors that overexpress P-gps. However, most common types of breast cancers, such as Luminal A, are low-P-gp expressing, at least during the initial phases of treatment. Therefore, it would be interesting to know if P-gp inhibitors are still useful in treating low-P-gp-expressing tumors.
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Development of an acellular tumor extracellular matrix as a three-dimensional scaffold for tumor engineering.
PLoS ONE
PUBLISHED: 01-01-2014
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Tumor engineering is defined as the construction of three-dimensional (3D) tumors in vitro with tissue engineering approaches. The present 3D scaffolds for tumor engineering have several limitations in terms of structure and function. To get an ideal 3D scaffold for tumor culture, A549 human pulmonary adenocarcinoma cells were implanted into immunodeficient mice to establish xenotransplatation models. Tumors were retrieved at 30-day implantation and sliced into sheets. They were subsequently decellularized by four procedures. Two decellularization methods, Tris-Trypsin-Triton multi-step treatment and sodium dodecyl sulfate (SDS) treatment, achieved complete cellular removal and thus were chosen for evaluation of histological and biochemical properties. Native tumor tissues were used as controls. Human breast cancer MCF-7 cells were cultured onto the two 3D scaffolds for further cell growth and growth factor secretion investigations, with the two-dimensional (2D) culture and cells cultured onto the Matrigel scaffolds used as controls. Results showed that Tris-Trypsin-Triton multi-step treated tumor sheets had well-preserved extracellular matrix structures and components. Their porosity was increased but elastic modulus was decreased compared with the native tumor samples. They supported MCF-7 cell repopulation and proliferation, as well as expression of growth factors. When cultured within the Tris-Trypsin-Triton treated scaffold, A549 cells and human colorectal adenocarcinoma cells (SW-480) had similar behaviors to MCF-7 cells, but human esophageal squamous cell carcinoma cells (KYSE-510) had a relatively slow cell repopulation rate. This study provides evidence that Tris-Trypsin-Triton treated acellular tumor extracellular matrices are promising 3D scaffolds with ideal spatial arrangement, biomechanical properties and biocompatibility for improved modeling of 3D tumor microenvironments.
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Baseline health-related quality of life and 10-year all-cause mortality among 1739 Chinese adults.
PLoS ONE
PUBLISHED: 01-01-2014
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Health-related quality of life (HRQOL) may be associated with the longevity of patients; yet it is not clear whether this association holds in a general population, especially in low- and middle-income countries. The objective of this study was to determine whether baseline HRQOL was associated with 10-year all-cause mortality in a Chinese general population.
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Large-scale genome-wide association studies and meta-analyses of longitudinal change in adult lung function.
PLoS ONE
PUBLISHED: 01-01-2014
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Genome-wide association studies (GWAS) have identified numerous loci influencing cross-sectional lung function, but less is known about genes influencing longitudinal change in lung function.
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Macrolide therapy in adults and children with non-cystic fibrosis bronchiectasis: a systematic review and meta-analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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A systematic review and meta-analysis was conducted to evaluate the efficacy and safety of macrolide therapy in adults and children with bronchiectasis.
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LIM-domain-only proteins: multifunctional nuclear transcription coregulators that interacts with diverse proteins.
Mol. Biol. Rep.
PUBLISHED: 01-01-2014
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The LIM-only subclass of LIM proteins is a family of nuclear transcription co-regulators that are characterized by the exclusive presence of two tandem LIM domains and no other functional domains. To date, four LIM-domain-only (LMO) proteins (LMO1-LMO4) have been identified. They regulate gene transcription by functioning as "linker" or "scaffolding" proteins with a remarkable potential to mediate protein-protein interactions. These proteins play important roles in cell fate determination, cell growth and differentiation, tissues patterning, and organ development. In this review, we briefly described the functions of LMO proteins in the organ development and diseases. We also summarized the interaction proteins of each LMO family member, which may contribute to elucidating the functions of these mysterious and important linker proteins.
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[Isolation of a methanol-utilizing strain and its application for determining methanol].
Wei Sheng Wu Xue Bao
PUBLISHED: 12-18-2013
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To isolate and characterize bacteria that can be used todevelop microbial biosensor for methanol (MeOH) determination.
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Bioinspired Helical Microswimmers Based on Vascular Plants.
Nano Lett.
PUBLISHED: 12-06-2013
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Plant-based bioinspired magnetically propelled helical microswimmers are described. The helical microstructures are derived from spiral water-conducting vessels of different plants, harnessing the intrinsic biological structures of nature. Geometric variables of the spiral vessels, such as the helix diameter and pitch, can be controlled by mechanical stretching for the precise fabrication and consistent performance of helical microswimmers. Xylem vessels of a wide variety of different plants have been evaluated for the consistency and reproducibility of their helical parameters. Sequential deposition of thin Ti and Ni layers directly on the spiral vessels, followed by dicing, leads to an extremely simple and cost-efficient mass-production of functional helical microswimmers. The resulting plant-based magnetic microswimmers display efficient propulsion, with a speed of over 250 ?m/s, as well as powerful locomotion in biological media such as human serum. The influence of actuation frequencies on the swimming velocity is investigated. Such use of plant vessels results in significant savings in the processing costs and provides an extremely simple, cost-effective fabrication route for the large-scale production of helical magnetic swimmers.
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[Current status of morning blood pressure control and medication of hypertensive patients in Beijing].
Zhonghua Xin Xue Guan Bing Za Zhi
PUBLISHED: 11-29-2013
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To explore the current status of morning blood pressure and medication of hypertensive patients in Beijing.
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[Enhancement of meniscal tearing damage repairing in the avascular zone using connective tissue growth factor (CTGF) in the rabbit model].
Zhongguo Gu Shang
PUBLISHED: 11-26-2013
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To investigate effect of connective tissue growth factors (CTGF) on secretion of extracellular matrix synthesis of meniscal fibrochondrocytes, expression of vascular endothelial growth factors (VEGF), and angiogenesis during the repair of meniscal tearing damage.
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In Situ High-Temperature Crystallographic Evolution of a Nonstoichiometric Li2O·2SiO2 Glass.
Inorg Chem
PUBLISHED: 11-22-2013
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In this work, the high-temperature crystallographic evolution of crystalline phases in a complex lithium disilicate glass was investigated using synchrotron X-ray powder diffraction. The lattice parameters and unit cell volume of Li2SiO3 (LS), Li2Si2O5 (LS2), Li3PO4 (LP), and ZrO2 as a function of temperature were determined upon heating. It is found that the lattice parameter c of LS2 shows a "V"-shaped trend during heating. The crystallographic evolution of the LS2 phase has a close correlation with the LS phase, indicating the mutual interaction between LS and LS2 phases along the c axis during the nucleation/crystallization process. The phase evolution processes were different upon heating and cooling, and the unit cell volume of both LS and LP phases demonstrated different change rates. In this glass system, no LS2 was detected during cooling and the main phases formed during cooling process were LP, LS, ?-cristobalite, and ?-quartz. Interestingly, there were two forms of ?-quartz with slightly different lattice constants, and the silica phases showed a near-zero expansion behavior. The crystallographic evolution mechanism is discussed.
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Consistent Anterior-Posterior Segregation of the Insula During the First 2 Years of Life.
Cereb. Cortex
PUBLISHED: 11-20-2013
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The human insula is a complex region characterized by heterogeneous cytoarchitecture, connectivity, and function. Subregional parcellation of the insula in adults has revealed an interesting anterior-posterior subdivision pattern that is highly consistent with its functional differentiation. However, the development of the insulas subregional segregation during the first 2 years of life remains unknown. The aim of this study was to test the hypothesis that similar segregation of the insula exists during this critical time period based on the resting-state functional magnetic resonance imaging study of a large cohort of infants (n = 143) with longitudinal scans. Our results confirmed a consistent anterior-posterior subdivision of the insula during the first 2 years of life with dissociable connectivity patterns associated with each cluster. Specifically, the anterior insula coupled more with frontal association areas, whereas the posterior insula integrated more with sensorimotor-related regions. More importantly, dramatic development of each subregions functional network was observed, providing important neuronal correlates for the rapid advancement of its related functions during this time period.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.