JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Bone substitutes enhance osteogenic differentiation of mesenchymal stem cells in three-dimensional scaffolds.
In Vivo
PUBLISHED: 09-06-2014
Show Abstract
Hide Abstract
The present study aimed to find bone substitutes to enhance osteogenic differentiation of mesenchymal stem cells in three-dimensional scaffolds in the absence of dexamethasone.
Related JoVE Video
Definition, etiology, prevention and treatment of peri-implantitis--a review.
Head Face Med
PUBLISHED: 09-03-2014
Show Abstract
Hide Abstract
Peri-implant inflammations represent serious diseases after dental implant treatment, which affect both the surrounding hard and soft tissue. Due to prevalence rates up to 56%, peri-implantitis can lead to the loss of the implant without multilateral prevention and therapy concepts. Specific continuous check-ups with evaluation and elimination of risk factors (e.g. smoking, systemic diseases and periodontitis) are effective precautions. In addition to aspects of osseointegration, type and structure of the implant surface are of importance. For the treatment of peri-implant disease various conservative and surgical approaches are available. Mucositis and moderate forms of peri-implantitis can obviously be treated effectively using conservative methods. These include the utilization of different manual ablations, laser-supported systems as well as photodynamic therapy, which may be extended by local or systemic antibiotics. It is possible to regain osseointegration. In cases with advanced peri-implantitis surgical therapies are more effective than conservative approaches. Depending on the configuration of the defects, resective surgery can be carried out for elimination of peri-implant lesions, whereas regenerative therapies may be applicable for defect filling. The cumulative interceptive supportive therapy (CIST) protocol serves as guidance for the treatment of the peri-implantitis. The aim of this review is to provide an overview about current data and to give advices regarding diagnosis, prevention and treatment of peri-implant disease for practitioners.
Related JoVE Video
Does pulsed magnetic field therapy influence nerve regeneration in the median nerve model of the rat?
Biomed Res Int
PUBLISHED: 07-21-2014
Show Abstract
Hide Abstract
The aim of this study was to evaluate the impact of pulsed magnetic field therapy on peripheral nerve regeneration after median nerve injury and primary coaptation in the rat. Both median nerves were surgically exposed and denervated in 24 female Wistar rats. A microsurgical coaptation was performed on the right side, whereas on the left side a spontaneous healing was prevented. The study group underwent a daily pulsed magnetic field therapy; the other group served as a control group. The grasping force was recorded 2 weeks after the surgical intervention for a period of 12 weeks. The right median nerve was excised and histologically examined. The histomorphometric data and the functional assessments were analyzed by t-test statistics and one-way ANOVA. One-way ANOVA indicated a statistically significant influence of group affiliation and grasping force (P = 0.0078). Grasping strength was higher on a significant level in the experimental group compared to the control group permanently from the 9th week to the end of the study. T-test statistics revealed a significantly higher weight of the flexor digitorum sublimis muscle (P = 0.0385) in the experimental group. The histological evaluation did not reveal any statistically significant differences concerning the histomorphometric parameters. Our results suggest that the pulsed magnetic field therapy has a positive influence on the functional aspects of neural regeneration. More studies are needed to precisely evaluate and optimize the intensity and duration of the application.
Related JoVE Video
Osteogenic differentiation of mesenchymal stem cells in fibrin-hydroxyapatite matrix in a 3-dimensional mesh scaffold.
In Vivo
PUBLISHED: 07-02-2014
Show Abstract
Hide Abstract
To explore the feasibility of culturing mesenchymal stem cells in an hydroxyapatite-fibrin matrix held by a mesh scaffold and inducing osteogenic differentiation of these cells. The aim was to obtain bone-material in vitro in a desired form.
Related JoVE Video
Melanotic and non-melanotic malignancies of the face and external ear - A review of current treatment concepts and future options.
Cancer Treat. Rev.
PUBLISHED: 04-01-2014
Show Abstract
Hide Abstract
Skin has the highest incidence and variety of tumors of all organs. Its structure is of great complexity, and every component has the potential to originate a skin neoplasm (SN). Because of its exposed nature, skin is vulnerable to carcinogenic stimuli such as UV radiation. Various entities can cause SN. Nonmelanotic skin cancers (NMSC) are the most common of all cancers, with over one million cases diagnosed annually in the US. Basal cell carcinoma (BCC) accounts for approximately 80% of all NMSC, most of the remaining 20% being squamous cell carcinoma (SCC). The skin of the head and neck is the most common site for tumors, accounting for more than 80% of all NMSC. BCC, SCC, and malignant melanomas (MM) represent 85-90% of all SN. Merkel cell tumors (MCC), lymphoepithelioma-like carcinomas of the skin (LELCS), dermato-fibro-sarcomas, leiomyosarkomas, and Kaposi-sarcomas are less frequent in the facial skin region and the external ear. Based on data from the German Federal Cancer Registry (2003/2004), 140,000 people in Germany were affected by SN (100,000 BCC, 22,000 SCC, 22,000 MM). This number increases considerably if malignant precursors, such as actinic keratosis, are included. Each year, the frequency of SN diagnosis rises by 3-7%. Among all known malignant tumors, MM exhibits the highest rate of increase in incidence. In the past, SN was primarily diagnosed in people aged 50 years or older. However, recently, the risk for developing SN has shifted, and younger people are also affected. Early diagnosis is significantly correlated with prognosis. Resection of SN creates defects that must be closed with local or microvascular flaps to avoid functional disturbing scar formation and deflection of the nose, eyelids, or lips. All therapeutic strategies for SN, the current standard for adjuvant and systemic treatment, and the management of the increasing number of patients under permanent blood thinner medication are described with regard to the treatment of SN.
Related JoVE Video
Microsurgical reconstruction of the head and neck - Current concepts of maxillofacial surgery in Europe.
J Craniomaxillofac Surg
PUBLISHED: 03-05-2014
Show Abstract
Hide Abstract
Microvascular surgery following tumour resection has become an important field of oral maxillofacial surgery (OMFS). This paper aims to evaluate current microsurgical practice in Europe.
Related JoVE Video
Face transplantation: on the verge of becoming clinical routine?
Biomed Res Int
PUBLISHED: 02-15-2014
Show Abstract
Hide Abstract
INTRODUCTION; Face transplantation (FT) is an innovative achievement of modern reconstructive surgery and is on the verge of becoming a common surgical opportunity. This review article was compiled to provide an update on this surgical field, especially regarding clinical outcomes, benefits, and complications implied.
Related JoVE Video
3D-tracking the regenerative potential of the mandible with micro-CTs.
Oral Maxillofac Surg
PUBLISHED: 02-11-2014
Show Abstract
Hide Abstract
The treatment of large bone defects is a challenging problem especially when the mandible is affected. Bone healing is dependent on the defect size and the integrity of periosteum. So far, these both aspects have not been investigated separately. The aim of this study was to evaluate the healing potential of the mandibular bone with the help of three-dimensional micro-computed tomography (CT).
Related JoVE Video
Outcome of microvascular free flaps in a high-volume training centre.
J Craniomaxillofac Surg
PUBLISHED: 02-10-2014
Show Abstract
Hide Abstract
Microvascular free tissue transfer allows major ablative defects following oncologic surgical and traumatic reasons to be reliably reconstructed in the head and neck region. A retrospective analysis of the microvascular flap procedures which were performed within one year in a high volume training centre was performed.
Related JoVE Video
Her2 expression and gene amplification is rarely detectable in patients with oral squamous cell carcinomas.
J. Oral Pathol. Med.
PUBLISHED: 01-30-2014
Show Abstract
Hide Abstract
Her2 (ErbB2) transforms cells when overexpressed and is an important therapeutic target in breast cancer. Contrary to breast cancer, studies on Her2 overexpression and gene amplification in squamous cell carcinomas of the head and neck region described largely different results. This study was undertaken to learn more on the prevalence and clinical significance of HER2 amplification and overexpression in squamous cell carcinomas of the head and neck.
Related JoVE Video
Virtual planning of complex head and neck reconstruction results in satisfactory match between real outcomes and virtual models.
Clin Oral Investig
PUBLISHED: 01-20-2014
Show Abstract
Hide Abstract
The reconstruction of large facial bony defects using microvascular transplants requires extensive surgery to achieve full rehabilitation of form and function. The purpose of this study is to measure the agreement between virtual plans and the actual results of maxillofacial reconstruction.
Related JoVE Video
From bench to application: Current practices in tissue engineering and its realisation at maxillofacial units in Germany, Austria and Switzerland.
J Craniomaxillofac Surg
PUBLISHED: 01-08-2014
Show Abstract
Hide Abstract
Over the last 20 years, the highly interdisciplinary field of tissue engineering (TE) has become an established subspecialty in research facilities all over the world. Numerous methods and protocols are available for various research intentions and aims, but there are no data indicating which of these methods and resources are generally used. This study is an overview of the resources and methods that are commonly applied in TE research in general, and in the field of oral and maxillofacial surgery (OMFS) in Germany, Austria and Switzerland. The DÖSAK collaborative group for TE developed a detailed questionnaire and collected information from participating university hospitals in these three countries. We evaluated the availability of research facilities, in vitro realisation and in vivo designs for animal studies in these departments. 11 units who replied, out of 35 we contacted, conducted research on bone regeneration in interdisciplinary research facilities. 10 departments used xenogeneic and alloplastic scaffolds for in vitro and in vivo applications. In this case, the most commonly utilised trademarks were Bio-Oss(®) and CERASORB(®). 9 units used osteoblasts (73%) and 10 proliferation assays in vitro, whereas rats served as the standard animal model for histology/immunohistochemistry in 6. All research units were interested in establishing a platform for research exchange and communication. This study shows that tissue engineering is well established and highly accepted in most participating university hospitals and research facilities. The presented data, together with data published in a foregoing paper will help arrange more readily available standardised procedures for further investigations.
Related JoVE Video
Morphometric study of the scapular free flap and the free rib osteomyocutaneous flap.
Br J Oral Maxillofac Surg
PUBLISHED: 01-06-2014
Show Abstract
Hide Abstract
The scapula free flap is often the first choice for reconstruction of bony defects of the facial skeleton. However, the vascularised rib as part of a free rib osteomyocutaneous flap may be a suitable second choice. We have investigated the morphology and clinical dimensions of the 7th rib and the scapula, and the ability of the available bone to carry dental implants. The age and sex of the cadaver, and the donor side, were also recorded. The dimensions of the scapulas and 7th ribs (n=130 of each) from 65 cadavers were measured at 4 different points using osteometric methods. Examination showed that bone from the scapula and 7th rib were sufficient for placement of implants. The 7th rib gave reliable measurements for both height and width, and a consistent relation between compact and cancellous bone. Although the scapula provided adequate compact and cancellous bone, there were variations depending on the segment of bone chosen. Bones from male cadavers were more suitable for implantation. In both the scapula and the 7th rib ageing had a significant adverse effect in only one dimension. Most points of measurement have satisfactory bony dimensions for insertion of dental implants.
Related JoVE Video
Incidence of bisphosphonate-related osteonecrosis of the jaw in consideration of primary diseases and concomitant therapies.
Anticancer Res.
PUBLISHED: 09-12-2013
Show Abstract
Hide Abstract
Since its first description by Marx in 2003, the etiology of bisphosphonate-related osteonecrosis of the jaw (BRONJ) is the subject of numerous scientific discussions for oral and maxillofacial surgeons. Many retrospective studies on its etiology and pathogenesis have been carried out to explain pathological mechanisms; most of them just take a close look at the issue of dosage and application. Recently, attempts have been made, to identify co-factors which might promote the development of BRONJ.
Related JoVE Video
Immunohistochemical and FISH analysis of EGFR and its prognostic value in patients with oral squamous cell carcinoma.
J. Oral Pathol. Med.
PUBLISHED: 07-16-2013
Show Abstract
Hide Abstract
To study immunohistochemical expression of the epithelial growth factor receptor (EGFR) in oral carcinomas and the head and neck region to examine possible associations with various features of the tumors and survival of the patients.
Related JoVE Video
The detection of oral pre- malignant lesions with an autofluorescence based imaging system (VELscopeTM) - a single blinded clinical evaluation.
Head Face Med
PUBLISHED: 07-02-2013
Show Abstract
Hide Abstract
The disease specific five-year survival rate especially for patients with advanced oral cancer has not improved significantly over the period of time. The most effective way of combating this dilemma is an early detection, diagnosis and eradication of early-stage lesions and their precursors. The use of VELscope® using an autofluorescence as a diagnostic tool might be useful in early detection of oral malignant lesions.
Related JoVE Video
Intraoperative efficiency of fluorescence imaging by Visually Enhanced Lesion Scope (VELscope(®)) in patients with bisphosphonate related osteonecrosis of the jaw (BRONJ).
J Craniomaxillofac Surg
PUBLISHED: 04-10-2013
Show Abstract
Hide Abstract
The purpose of this study was to determine the potential of tissue fluorescence imaging by using Visually Enhanced Lesion Scope (VELscope(®)) for the detection of osteonecrosis of the jaw induced by bisphosphonates (BRONJ).
Related JoVE Video
CCND1 amplification and cyclin D1 immunohistochemical expression in head and neck squamous cell carcinomas.
Clin Oral Investig
PUBLISHED: 03-04-2013
Show Abstract
Hide Abstract
Gene products, which show a significant association to cell proliferation and cell cycle control, are of high scientific interest, because genes as well as gene products could be possible targets for a specific therapeutic approach and eventually be prognostic markers.
Related JoVE Video
Postoperative bleeding risk for cutaneous surgery in the head and neck region with continued phenprocoumon therapy.
J Craniomaxillofac Surg
PUBLISHED: 02-20-2013
Show Abstract
Hide Abstract
In a total of 171 surgical procedures for lesions in the head and neck region in patients in whom phenprocoumon therapy was not stopped, 16 (9%) postoperative bleeding events were observed over a follow-up period of two weeks. Local measures were sufficient in all cases except one severe case where blood transfusion was needed and anticoagulant treatment was stopped for 7 days. The bleeding risk was significantly higher for the surgical procedures of the nose than those in other areas (21% versus 6%, P = 0.014), but was not influenced by the international normalized ratio (INR) of blood coagulation, size, site and type of the lesion, surgical procedure, and sex and age of the patients. The bleeding rate in patients not on any anticoagulation therapy was significantly lower (6/211 = 3%). Across both groups, just over 80% of the bleeding episodes were within the first two days (55% on the same day and 32% on the next day) of the surgery. No bleeding was recorded after 5 days. Our data suggest that cutaneous surgery in the head and neck region can be safely performed with continued phenprocoumon therapy in most cases in an INR range of 1.3-3.4, but rarely severe bleeding does occur and can be managed with a close-contact follow-up and with 24-h on call services during the first two days postoperatively.
Related JoVE Video
Primary and secondary leiomyosarcoma of the oral and perioral region--clinicopathological and immunohistochemical analysis of a rare entity with a review of the literature.
J. Oral Maxillofac. Surg.
PUBLISHED: 02-20-2013
Show Abstract
Hide Abstract
Leiomyosarcoma (LMS) rarely occurs in the head and neck region. These tumors present with a wide range of clinical features, so the diagnosis is predicated on conventional microscopic findings coupled with immunohistochemical analysis.
Related JoVE Video
Apicoectomy using Er:YAG laser in association with microscope: a comparative retrospective investigation.
Photomed Laser Surg
PUBLISHED: 02-19-2013
Show Abstract
Hide Abstract
This retrospective clinical investigation intends to examine the effectiveness of an Er:YAG laser used in conjunction with a dental operation microscope for apicoectomy, in comparison with the traditional surgical procedure.
Related JoVE Video
The NTPase/helicase domain of hepatitis C virus nonstructural protein 3 inhibits protein kinase C independently of its NTPase activity.
Cell. Mol. Biol. Lett.
PUBLISHED: 02-05-2013
Show Abstract
Hide Abstract
Helicase motif VI is a short arginine-rich motif within the NTPase/helicase domain of the non-structural protein 3 (NS3) of the hepatitis C virus (HCV). We previously demonstrated that it reduces the catalytic activity and intracellular shuttling of protein kinase C (PKC). Thus, NS3-mediated PKC inhibition may be involved in HCV-associated hepatocellular carcinoma (HCC). In this study, we expand on our earlier results, which were obtained in experiments with short fragments of NS3, to show for the first time that the catalytically active, longer C-terminal NTPase/helicase of NS3 acts as a potent PKC inhibitor in vitro. PKC inhibition assays with the NTPase-inactive mutant NS3h-D1316A revealed a mixed type kinetic inhibition pattern. A broad range of 11 PKC isotypes was tested and all of the PKC isotypes were inhibited with IC??-values in the low micromolar range. These findings were confirmed for the wild-type NTPase/helicase domain in a non-radiometric PKC inhibition assay with ATP regeneration to rule out any effect of ATP hydrolysis caused by its NTPase activity. PKC? was inhibited with a micromolar IC?? in this assay, which compares well with our result for NS3h-D1316A (IC?? = 0.7 ?M). In summary, these results confirm that catalytically active NS3 NTPase/helicase can act in an analogous manner to shorter NS3 fragments as a pseudosubstrate inhibitor of PKC.
Related JoVE Video
P53 immunohistochemical expression does not correlate with clinical features in 207 carcinomas of the oral cavity and in the head and neck region.
Clin Oral Investig
PUBLISHED: 01-28-2013
Show Abstract
Hide Abstract
The present study aims to investigate the relevance of immunohistochemical p53 expression in carcinomas of the oral cavity and of the head and neck region. Long-term clinical and histopathological follow-up findings as well as HPV status are correlated with the results of this examination.
Related JoVE Video
Mandibular cortical shape index in non-standardised panoramic radiographs for identifying patients with osteoporosis as defined by the German Osteology Organization.
J Craniomaxillofac Surg
PUBLISHED: 01-26-2013
Show Abstract
Hide Abstract
The aim of this prospective controlled study was to determine the validity of the mandibular cortical shape index (CI) on panoramic radiographs in distinguishing patients with osteoporosis as defined by the German Osteology Organization (Dachverbands der Deutschsprachigen Osteologischen Fachgesellschaften, DVO). The study group contained 50 patients (33 female, 17 male, mean age: 74.9 years), who had a high risk of osteoporosis. 50 young patients (33 female, 17 male, mean age: 37.9 years) with no anamnestic evidence of osteoporosis served as control. Three blinded observers evaluated panoramic views of the study and the control group in a mixed manner regarding the mandibular cortical shape index. The study group underwent bone mineral density measurement using dual energy X-ray absorptiometry and received a diagnosis according to the Organizations guidelines (normal, osteopenia, osteoporosis). The CI showed a high sensitivity of 72.2% and a high specificity of 93.9% with a highly significant predictive value (Chi-square = 22.96, p < 0.001), while the observers agreement was moderate (kappa = 0.47). We concluded that patients with a cortical shape index of the category assessed as "severe" on non-standardised panoramic radiographs have a higher risk of suffering systemic osteoporosis. The CI in panoramic radiographs is a good screening tool, which could be implemented in the routine assessment of panoramic radiographs in elder patients.
Related JoVE Video
Neutrophil-derived cathelicidin protects from neointimal hyperplasia.
Sci Transl Med
PUBLISHED: 10-07-2011
Show Abstract
Hide Abstract
Percutaneous transluminal angioplasty with stent implantation is used to dilate arteries narrowed by atherosclerotic plaques and to revascularize coronary arteries occluded by atherothrombosis in myocardial infarction. Commonly applied drug-eluting stents release antiproliferative or anti-inflammatory agents to reduce the incidence of in-stent stenosis. However, these stents may still lead to in-stent stenosis; they also show increased rates of late stent thrombosis, an obstacle to optimal revascularization possibly related to endothelial recovery. Here, we examined the contribution of neutrophils and neutrophilic granule proteins to arterial healing after injury. We found that neutrophil-borne cathelicidin (mouse CRAMP, human LL-37) promoted reendothelization and thereby limited neointima formation after stent implantation. We then translated these findings to an animal model using a neutrophil-instructing, biofunctionalized, miniaturized Nitinol stent coated with LL-37. This stent reduced in-stent stenosis in a mouse model of atherosclerosis, suggesting that LL-37 may promote vascular healing after interventional therapy.
Related JoVE Video
Effect of oxidized regenerated cellulose/collagen matrix on proteases in wound exudate of patients with diabetic foot ulcers.
J Wound Ostomy Continence Nurs
PUBLISHED: 08-24-2011
Show Abstract
Hide Abstract
The aim of this study was to investigate the influence of oxidized regenerated cellulose/collagen matrix on the concentration and activity of gelatinases, elastase, and plasmin in wound exudate.
Related JoVE Video
Manufacturing of individual biodegradable bone substitute implants using selective laser melting technique.
J Biomed Mater Res A
PUBLISHED: 04-14-2011
Show Abstract
Hide Abstract
The additive manufacturing technique selective laser melting (SLM) has been successfully proved to be suitable for applications in implant manufacturing. SLM is well known for metal parts and offers direct manufacturing of three-dimensional (3D) parts with high bulk density on the base of individual 3D data, including computer tomography models of anatomical structures. Furthermore, an interconnecting porous structure with defined and reproducible pore size can be integrated during the design of the 3D virtual model of the implant. The objective of this study was to develop the SLM processes for a biodegradable composite material made of ?-tricalcium phosphate (?-TCP) and poly(D, L)-lactide (PDLLA). The development of a powder composite material (?-TCP/PDLLA) suitable for the SLM process was successfully performed. The microstructure of the manufactured samples exhibit a homogeneous arrangement of ceramic and polymer. The four-point bending strength was up to 23 MPa. The X-ray diffraction (XRD) analysis of the samples confirmed ?-TCP as the only present crystalline phase and the gel permeations chromatography (GPC) analysis documented a degradation of the polymer caused by the laser process less than conventional manufacturing processes. We conclude that SLM presents a new possibility to manufacture individual biodegradable implants made of ?-TCP/PDLLA.
Related JoVE Video
Complications after oral surgery in patients with congenital or drug-induced bleeding disorders.
In Vivo
PUBLISHED: 04-08-2011
Show Abstract
Hide Abstract
Bleeding disorders (BD) can cause significant problems during or after surgical procedures of the oral cavity. The aim of this study was to explore the risk of bleeding following oral surgery in patients with BD.
Related JoVE Video
Verification of nerve integrity after surgical intervention using quantitative sensory testing.
J. Oral Maxillofac. Surg.
PUBLISHED: 03-20-2011
Show Abstract
Hide Abstract
The aim of this study was to apply a standardized Quantitative Sensory Testing (QST) approach in patients to investigate whether oral surgery can lead to sensory changes, even if the patients do not report any sensory disturbances. Furthermore, this study determines the degree and duration of possible neuronal hyperexcitability due to local inflammatory trauma after oral surgery.
Related JoVE Video
The influence of bisphosphonates on human osteoblast migration and integrin aVb3/tenascin C gene expression in vitro.
Head Face Med
PUBLISHED: 02-07-2011
Show Abstract
Hide Abstract
Bisphosphonates are therapeutics of bone diseases, such as Pagets disease, multiple myeloma or osteoclastic metastases. As a severe side effect the bisphosphonate induced osteonecrosis of the jaw (BONJ) often requires surgical treatment and is accompanied with a disturbed wound healing.Therefore, the influence on adhesion and migration of human osteoblasts (hOB) after bisphosphonate therapy has been investigated by morphologic as well as gene expression methods.
Related JoVE Video
Adhesion molecule L1 is down-regulated in malignant peripheral nerve sheath tumors versus benign neurofibromatosis type 1-associated tumors.
Oral Surg Oral Med Oral Pathol Oral Radiol
PUBLISHED: 01-06-2011
Show Abstract
Hide Abstract
Type 1 neurofibromatosis (NF-1), also known as von Recklinghausen disease, is caused by a disorder of a single gene on chromosome 17 that usually restrains cell division. A sequence that is frequently associated with NF-1 is tumor progression from neurofibromas to malignant peripheral nerve sheath tumors (MPNSTs). The aim of this study was to determine the expression of the neural L1 cell adhesion molecule in dermal-diffuse neurofibromas, plexiform neurofibromas, and MPNSTs of NF-1. We retrospectively analyzed surgically resected primary tumors, including 20 dermal neurofibromas, 23 plexiform neurofibromas, and 17 MPNSTs, by immunohistochemistry in paraffin sections of NF-1 tumors with the use of the L1-specific monoclonal antibody UJ127, which does not cross-react with other members of the L1 family. Immunostainings for CD34 and S100 were included to distinguish and allocate L1-expressing Schwann cells and perineural (specialized) fibroblasts. Our data showed that L1 is highly expressed in all benign NF-1 tumors and in some but not all MPNSTs. Furthermore, we demonstrated a correlation between L1 expression and differentiation grade of MPNSTs. There was a significant trend toward lower or nondetectable expression in the poorly differentiated MPNSTs, in contrast to all other tumor entities so far investigated, in which L1 expression correlated positive with malignancy, except for juvenile but not adult-derived neuroblastomas. Future studies are warranted to elucidate the molecular basis of the varying effects of the degree of L1 expression, receptor, and signal transduction mechanisms in different tumors.
Related JoVE Video
Additive manufacturing for microvascular reconstruction of the mandible in 20 patients.
J Craniomaxillofac Surg
PUBLISHED: 01-04-2011
Show Abstract
Hide Abstract
The aim of this study was to evaluate the use of model mandibles made preoperatively by additive manufacturing, which were used to prebend reconstruction plates prior to mandibular resection and reconstruction with microvascular bony flaps.
Related JoVE Video
In vitro study of adhesive polymethylmethacrylate bone cement bonding to cortical bone in maxillofacial surgery.
J. Oral Maxillofac. Surg.
PUBLISHED: 10-23-2010
Show Abstract
Hide Abstract
In the treatment of midface fractures, the fragments are immobilized using screws and plates for osteosynthesis until reunion has occurred. This method involves drilling holes for the insertion of the screws, which can be associated with additional fracturing of the corresponding bone owing to the complex architecture and thin layers of facial bone. To alleviate this problem, new adhesive techniques for fixing the plates for osteosynthesis have been investigated, mitigating the detrimental effects of screw hole drilling. In the present experimental study, the strength of this adhesive bond and its resistance to hydrolysis were investigated.
Related JoVE Video
A novel hemostatic delivery device for thrombin: biodegradable poly(D,L-lactide-co-glycolide) 50:50 microspheres.
J Biomed Mater Res A
PUBLISHED: 07-28-2010
Show Abstract
Hide Abstract
Topical thrombins are locally active hemostatic agents that can be used to minimize blood loss during any surgery. The aim of this study was to design and investigate a thrombin-containing biodegradable hemostyptic device with an optimized drug release profile to promote local blood clot formation. It is effective with ongoing systemic antithrombotic therapy and can be used in all types of bone-related surgery, for example, in dental surgery. Thrombin-loaded poly(D,L-lactide-co-glycolide) microspheres were synthesized by means of complex (w/o/w) emulsion evaporation method. The resulting enzyme activity of the serine-protease thrombin was verified by the specific chromogenic substrate S-2238. The thrombin release profile depended on four factors: (1) thrombin dosage, (2) polymer concentration in the o-phase, (3) phase quotient w1:0 in the primary emulsion, and (4) the addition of pore-introducing agents. A collagenous sponge containing thrombin-loaded microspheres by means of lyophilization was developed. The impact of several production factors of the (w1/o/w2) solvent evaporation method to optimize thrombin encapsulation, morphology of the spheres, and desired drug release profile have been investigated. The in vitro thrombin release was dependent on the polymer-to-oil phase ratio, the polymer concentration, and the type of solvent and polymer. The porosity of the spheres and release rate of the active agent were enhanced by increasing the inner aqueous w1 phase. With this study, a new biodegradable hemostyptic device could be verified and established for a potentially safe and locally controlled thrombin release to manage postsurgical hemorrhage in patients undergoing anticoagulant therapy.
Related JoVE Video
The impact of nonautogenous bone graft on orthodontic treatment.
Quintessence Int
PUBLISHED: 07-27-2010
Show Abstract
Hide Abstract
Guided bone regeneration with alloplastic bone graft substitutes has a high impact in surgical dentistry nowadays. In the future, the orthodontist will inevitably be confronted with patients with such a medical history. A review of the literature was performed to investigate present materials used, indications, and concerns and problems in interdisciplinary treatment of patients with nonautogenous bone grafts.
Related JoVE Video
Assessment of trigeminal nerve functions by quantitative sensory testing in patients and healthy volunteers.
J. Oral Maxillofac. Surg.
PUBLISHED: 07-03-2010
Show Abstract
Hide Abstract
Orofacial sensory dysfunction plays an important role in oral and maxillofacial surgery. Quantitative sensory testing (QST) is a psychophysical approach to evaluate thermal and mechanical somatosensation.
Related JoVE Video
Analysis of trigeminal nerve disorders after oral and maxillofacial intervention.
Head Face Med
PUBLISHED: 06-24-2010
Show Abstract
Hide Abstract
Quantitative sensory testing (QST) is applied to evaluate somatosensory nerve fiber function in the spinal system. This study uses QST in patients with sensory dysfunctions after oral and maxillofacial surgery.
Related JoVE Video
Gender specific quality of life in patients with oral squamous cell carcinomas.
Head Face Med
PUBLISHED: 05-17-2010
Show Abstract
Hide Abstract
The goal of this study was to evaluate the somatic and psychological effects by means of QUALITY OF LIFE (QOL) of surgical treatment of patients with oral squamous cell carcinoma. The factors gender, age, nicotine consumption, and tumour stage were taken into consideration.
Related JoVE Video
The impact of bisphosphonates on the osteoblast proliferation and Collagen gene expression in vitro.
Head Face Med
PUBLISHED: 04-19-2010
Show Abstract
Hide Abstract
Bisphosphonates are widely used in the clinical treatment of bone diseases with increased bone resorption. In terms of side effects, they are known to be associated with osteonecrosis of the jaw (BONJ).The objective of this study was to evaluate the effect of bisphosphonates on osteoblast proliferation by cell count and gene expression analysis of cyclin D1 in vitro. Furthermore, the gene expression of the extracellular matrix protein collagen type I was evaluated. Nitrogen-containing and non-nitrogen-containing bisphosphonates have been compared on gene expression levels.
Related JoVE Video
Influence of bisphosphonates on the osteoblast RANKL and OPG gene expression in vitro.
Clin Oral Investig
PUBLISHED: 03-06-2010
Show Abstract
Hide Abstract
Bisphosphonates are widely used in the clinical treatment of bone diseases with increased bone resorption. In terms of side effects, they are widely known to be associated with osteonecrosis of the jaw (BONJ). The objective of this study was to evaluate the effect of bisphosphonates on the gene expression of receptor activator of NF-?B ligand (RANKL) and osteoprotegerin (OPG) in vitro. Nitrogen-containing and non-nitrogen containing bisphosphonates have been compared. Human osteoblasts were stimulated with zoledronate and ibandronate at concentrations of 5 × 10(-5) M, 5 × 10(-6) M, and 5 × 10(-7) M over the experimental period of 14 days. Furthermore, the hOB cell lines were stimulated by clodronate at concentrations of 5 × 10(-3) M, 5 × 10(-5) M, and 5 × 10(-6) M. At each point in time, the gene expression levels of RANKL and OPG were quantified by real-time RT-PCR. The results showed a moderate enhancement of OPG gene expression whereas RANKL gene expression was strongly increased by nitrogen-containing bisphosphonates reaching a maximum after 14 days at high concentrations of 5 × 10(-5) M. Lower concentrations did not enhance the RANKL and OPG expression considerably. The non-nitrogen-containing bisphosphonate clodronate, however, effected OPG and RANKL gene expression much less, even at higher concentrations of 5 × 10(-3) M. The above-mentioned data suggest an enhanced RANKL/OPG gene expression after stimulation by bisphosphonates. Interestingly, clodronate might have little influence on osteoblast/osteoclast interaction with respect to OPG and RANKL gene expression.
Related JoVE Video
Zoledronate, ibandronate and clodronate enhance osteoblast differentiation in a dose dependent manner--a quantitative in vitro gene expression analysis of Dlx5, Runx2, OCN, MSX1 and MSX2.
J Craniomaxillofac Surg
PUBLISHED: 01-17-2010
Show Abstract
Hide Abstract
Bisphosphonates are widely used in the clinical treatment of bone diseases with increased bone resorption. In terms of side effects, they are known to be associated with osteonecrosis of the jaw (BONJ). There are two groups of bisphosphonates: the nitrogen-containing bisphosphonates, e.g. zoledronate and ibandronate, and the non-nitrogen-containing bisphosphonates, e.g. clodronate. Their impact on bone metabolism seems to differ. The objective of this study was to compare the osteogenic differentiation potency of these two pharmacologic groups. Human osteoblasts were stimulated with zoledronate and ibandronate at concentrations of 5×10(-5) M, 5×10(-6) M and 5×10(-7) M over the experimental periods of 1, 2, 5, 10 and 14 days. Clodronate was applied with concentrations of 5×10(-3), 5×10(-5) M and 5×10(-6) M. At each time point, the cells were dissolved, the mRNA extracted, and the gene expression level of the osteoblast specific differentiation markers of the homeobox transcription factors MSX1 and MSX2, the distal-less homeobox 5 (Dlx5), the Runt-related transcription factor 2 (Runx2/CBF1a) and osteocalcin (OCN) were quantified by Real-Time PCR. The gene expression was compared to an unstimulated osteoblast cell culture as control. The results showed a significant difference between the nitrogen-containing and the non-nitrogen-containing bisphosphonates. Zoledronate and ibandronate at concentrations of 5×10(-5) M enhanced the gene expression of all differentiation markers by several hundred folds compared to unstimulated control after 10 days, whereas clodronate had less influence on gene expression, even at higher concentrations of 5×10(-3) M. Lower concentrations of zoledronate and ibandronate, however, led to a decreased gene expression. These data confirm the results of other studies which have shown the osteogenic stimulus on osteoblasts in a dose dependent manner. The nitrogen-containing bisphosphonates appear to enhance bone density by stimulation of osteoblast differentiation. Non-nitrogen-containing bisphosphonates seem to have less influence on osteoblast differentiation.
Related JoVE Video
Clinical evaluation of a biphasic calcium composite grafting material in the treatment of human periodontal intrabony defects: a 12-month randomized controlled clinical trial.
J. Periodontol.
PUBLISHED: 11-13-2009
Show Abstract
Hide Abstract
The aim of this study was to compare the clinical outcome of a novel biphasic calcium composite (BCC) biomaterial versus autogenous bone spongiosa (ABS) or open flap debridement (OFD) for the treatment of intrabony periodontal defects.
Related JoVE Video
Clinical periodontal and microbiologic parameters in patients with acute myocardial infarction.
J. Periodontol.
PUBLISHED: 10-02-2009
Show Abstract
Hide Abstract
The aim of this study was to evaluate the impact of clinical periodontal parameters and the presence of periodontal pathogens in patients with acute myocardial infarction (AMI).
Related JoVE Video
The role of the composite interleukin-1 genotype in the association between periodontitis and acute myocardial infarction.
J. Periodontol.
PUBLISHED: 07-01-2009
Show Abstract
Hide Abstract
Recent data indicate that interleukin (IL)-1 polymorphism may influence the susceptibility to periodontitis and coronary heart diseases. The aim of this study was to evaluate the impact of the composite IL-1 genotype (allele 2 at IL-1A -889 and IL-1B +3954) in the association between acute myocardial infarction (AMI) and periodontitis.
Related JoVE Video
A new biphasic osteoinductive calcium composite material with a negative Zeta potential for bone augmentation.
Head Face Med
PUBLISHED: 06-13-2009
Show Abstract
Hide Abstract
The aim of the present study was to analyze the osteogenic potential of a biphasic calcium composite material (BCC) with a negative surface charge for maxillary sinus floor augmentation. In a 61 year old patient, the BCC material was used in a bilateral sinus floor augmentation procedure. Six months postoperative, a bone sample was taken from the augmented regions before two titanium implants were inserted at each side. We analyzed bone neoformation by histology, bone density by computed tomography, and measured the activity of voltage-activated calcium currents of osteoblasts and surface charge effects. Control orthopantomograms were carried out five months after implant insertion. The BCC was biocompatible and replaced by new mineralized bone after being resorbed completely. The material demonstrated a negative surface charge (negative Zeta potential) which was found to be favorable for bone regeneration and osseointegration of dental implants.
Related JoVE Video
In vitro proliferation of human osteogenic cells in presence of different commercial bone substitute materials combined with enamel matrix derivatives.
Head Face Med
PUBLISHED: 05-23-2009
Show Abstract
Hide Abstract
Cellular reactions to alloplastic bone substitute materials (BSM) are a subject of interest in basic research. In regenerative dentistry, these bone grafting materials are routinely combined with enamel matrix derivatives (EMD) in order to additionally enhance tissue regeneration.
Related JoVE Video
Impact of rhBMP-2 on regeneration of buccal alveolar defects during the osseointegration of transgingival inserted implants.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
PUBLISHED: 05-07-2009
Show Abstract
Hide Abstract
New approaches to enhance vertical bone regeneration in clinically relevant implant models are needed. Therefore, we analyzed the impact of recombinant human bone morphogenic protein 2 (rhBMP-2) on the healing of large buccal alveolar defects during osseointegration of transgingivally inserted implants.
Related JoVE Video
The impact of defined polyglycolide scaffold structure on the proliferation of gingival fibroblasts in vitro: a pilot study.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
PUBLISHED: 04-02-2009
Show Abstract
Hide Abstract
The postoperative apical growth of epithelium on teeth is a common complication of surgical periodontal therapy. Absorbable textile scaffolds, placed between the operated tooth and its nearby tissues, are considered to serve as a mechanical barrier and prevent undesired apical epithelial growth. They allow local regeneration and the new formation of human gingival fibroblasts when the scaffolds are colonized with autologous cells. The aim of this study was to examine the proliferation behavior of human gingival fibroblasts on polyglycolic acid (PGA) fleeces with various structural characteristics.
Related JoVE Video
Neural differentiation potential of human bone marrow-derived mesenchymal stromal cells: misleading marker gene expression.
BMC Neurosci
PUBLISHED: 03-03-2009
Show Abstract
Hide Abstract
In contrast to pluripotent embryonic stem cells, adult stem cells have been considered to be multipotent, being somewhat more restricted in their differentiation capacity and only giving rise to cell types related to their tissue of origin. Several studies, however, have reported that bone marrow-derived mesenchymal stromal cells (MSCs) are capable of transdifferentiating to neural cell types, effectively crossing normal lineage restriction boundaries. Such reports have been based on the detection of neural-related proteins by the differentiated MSCs. In order to assess the potential of human adult MSCs to undergo true differentiation to a neural lineage and to determine the degree of homogeneity between donor samples, we have used RT-PCR and immunocytochemistry to investigate the basal expression of a range of neural related mRNAs and proteins in populations of non-differentiated MSCs obtained from 4 donors.
Related JoVE Video
Intraoperative cone-beam computed tomography in oral and maxillofacial surgery using a C-arm prototype: first clinical experiences after treatment of zygomaticomaxillary complex fractures.
J. Oral Maxillofac. Surg.
PUBLISHED: 02-24-2009
Show Abstract
Hide Abstract
To describe the first clinical applications of intraoperative cone-beam computed tomography with an integrated flat-panel detector in oral and maxillofacial surgery after surgical treatment of zygomaticomaxillary complex fractures
Related JoVE Video
The innovative application of a novel bone adhesive for facial fracture osteosynthesis-in vitro and in vivo results.
J Biomed Mater Res A
Show Abstract
Hide Abstract
This study evaluates a novel adhesive fixation technique to affix cortical bone fragments to osteosynthesis plates using common PMMA cement. This technique utilizes a new amphiphilic bone bonding agent adhering with both hydrophilic bone and hydrophobic PMMA cement. After in vitro biomechanical testing of the bonding strength with explanted bovine and rabbit calvarian bone samples, osteosynthesis plates with screw holes of 1.3 and 1.5 mm were placed on the cranial bone of New Zealand white rabbits and the bond strength of these plates was determined through tension tests. In vitro bond strengths of 19.8-26.5 MPa were obtained. Control samples, prepared without a bone bonding agent, exhibited bone bonding strengths <0.2 MPa. In vivo respective bond strengths at the cranium of the white rabbits were 2.5-4.1 MPa 2 weeks post surgery and 1.9-2.5 MPa 12 weeks after implantation. This new innovative fixation method can be envisioned for cases in which conventional fixation techniques of screws and plates are insufficient or not possible due to the bone or trauma conditions. The observed bonding strengths support implementing this technique in nonload bearing regions, such as the central midface or frontal sinus, facilitating immobilization until bone reunion is complete.
Related JoVE Video
Scaffold-free microtissues: differences from monolayer cultures and their potential in bone tissue engineering.
Clin Oral Investig
Show Abstract
Hide Abstract
Cell-based therapies for bone augmentation after tooth loss and for the treatment of periodontal defects improve healing defects. Usually, osteogenic cells or stem cells are cultivated in 2D primary cultures, before they are combined with scaffold materials, even though this means a loss of the endogenous 3D microenvironment for the cells. Moreover, the use of single-cell suspensions for the inoculation of scaffolds or for the direct application into an area of interest has the disadvantages of low initial cell numbers and susceptibility to unwanted cellular distribution, respectively.
Related JoVE Video
Tissue expanders for soft tissue reconstruction in the head and neck area--requirements and limitations.
Clin Oral Investig
Show Abstract
Hide Abstract
The repair of skin defects in the head and neck region still poses a significant problem for many clinicians. Tissue expansion is described as a treatment option providing good color, texture, and thickness match of the expanded skin. Unfortunately, the complication rates for tissue expansion range from 0 to 48 %. Therefore, the purpose of this study was to investigate risk factors for the use of tissue expanders in head and neck reconstructions.
Related JoVE Video
Endocultivation: metabolism during heterotopic osteoinduction in vivo--monitoring with fiber optic detection devices.
Tissue Eng Part C Methods
Show Abstract
Hide Abstract
Reconstructions of facial bone defects are one of the most challenging aspects in surgical treatment of malignant diseases, large facial traumata, or congenital anomalies. High-level reconstruction techniques are often associated with an elevated morbidity by the harvest of autologous bone grafts from the patient. Tissue engineering techniques may help to solve this problem. The aim of this study was to monitor metabolic processes during cellular colonization of matrices in vivo in an established rat model for endocultivation. After implantation of computer-designed hydroxyapatite scaffolds into the latissimus dorsi muscle of six rats, 100??g bone morphogenetic protein-2 (BMP-2) was injected twice, in week 1 and 2, directly into the center of the matrices. The development of pH value and oxygen (O?) saturation inside the matrix was followed by fiber optic detection technique over 8 weeks and analyzed by variance analyses. Bone density measurements were performed by computed tomography as well as histological evaluations. Two weeks after implantation, oxygen supply and pH value measurements had decreased significantly. In the following weeks both parameters increased and stabilized on higher levels. This is the first study reporting a reproducible method to follow metabolic processes during heterotopic osteoinduction in vivo. It was shown that in the beginning of the study pH value and O? saturation decreased and it took several weeks to regain physiological levels. This is an important step to further understand the physiological process of bone induction.
Related JoVE Video
Biocompatibility and biodegradation of a native porcine pericardium membrane: results of in vitro and in vivo examinations.
Int J Oral Maxillofac Implants
Show Abstract
Hide Abstract
The principle of guided tissue and bone regeneration has long been established for treating bone and periodontal defects. In addition to nonabsorbable barriers, various collagens have become accepted as membrane materials for separating the graft from soft tissue. However, collagen membranes differ significantly in their barrier function and biocompatibility. The objective of this pilot study was to examine, in vitro and in vivo, a novel native collagen membrane extracted from porcine pericardium.
Related JoVE Video
Current trends and future perspectives of bone substitute materials - from space holders to innovative biomaterials.
J Craniomaxillofac Surg
Show Abstract
Hide Abstract
An autologous bone graft is still the ideal material for the repair of craniofacial defects, but its availability is limited and harvesting can be associated with complications. Bone replacement materials as an alternative have a long history of success. With increasing technological advances the spectrum of grafting materials has broadened to allografts, xenografts, and synthetic materials, providing material specific advantages. A large number of bone-graft substitutes are available including allograft bone preparations such as demineralized bone matrix and calcium-based materials. More and more replacement materials consist of one or more components: an osteoconductive matrix, which supports the ingrowth of new bone; and osteoinductive proteins, which sustain mitogenesis of undifferentiated cells; and osteogenic cells (osteoblasts or osteoblast precursors), which are capable of forming bone in the proper environment. All substitutes can either replace autologous bone or expand an existing amount of autologous bone graft. Because an understanding of the properties of each material enables individual treatment concepts this review presents an overview of the principles of bone replacement, the types of graft materials available, and considers future perspectives. Bone substitutes are undergoing a change from a simple replacement material to an individually created composite biomaterial with osteoinductive properties to enable enhanced defect bridging.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.