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Find video protocols related to scientific articles indexed in Pubmed.
[Effect of different types of jumps on the expressions of IL-6, OPG and RANKL in rat tibia].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
PUBLISHED: 09-10-2014
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Objective To investigate the effect of different types of jumps on the expressions of interleukin 6 (IL-6), osteoprotegerin(OPG), ligand of receptor activator of NF-?B(RANKL) genes related to bone metabolism in rat tibia, and explore the molecular mechanism underlying bone destruction associated with over-loading jumping. Methods Twenty-four male SD rats were randomly assigned to control group (C), jump group (J) and loading jump group (JL), 8 rats in each group. J group participated in incremental load training. The rats in JL group carried loads that were 5% of their body mass. Same training plan was used in J and JL groups, while control group was not treated. The experiment was completed after 6 weeks. The serum samples were collected and assayed for the contents of alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRACP). One-side tibia was used for paraffin section and HE staining, and the other side was used for real time quantitative PCR(qRT-PCR) to detect the expressions of IL-6, OPG and RANKL mRNA. Results Compared with control group and J group, the collagen fibers in periost was more disordered in JL group. We further observed a more serious damage of the cortical bone and increased cavities in JL group. The serum ALP levels in JL group were significantly higher than those in control and J groups (P<0.05). The serum TRACP levels in JL and J groups were significantly higher compared to control group (P<0.05). Furthermore, there was a significantly higher expression level of IL-6 in both jump groups (P<0.01), while the JL group had a higher IL-6 expression compared with J group (P<0.01). There was a higher expression of OPG mRNA in JL and J groups (P<0.05). The highest expression level of OPG was observed in J group. The expression level of RANKL was significantly higher in JL group compared with control and J groups (P<0.01). Conclusion After loading jump for 6 weeks, the expressions of IL-6 and RANKL were markedly elevated in rat tibia, suggesting that IL-6 and RANKL might be involved in the process of bone destruction due to increased bone turnover.
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In vivo ultrasound molecular imaging of inflammatory thrombosis in arteries with cyclic Arg-Gly-Asp-modified microbubbles targeted to glycoprotein IIb/IIIa.
Invest Radiol
PUBLISHED: 07-17-2013
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Ultrasound molecular imaging has the potential to detect activated platelets, thus identifying atherosclerotic plaque instability before onset of serious clinical events. However, it has not been well defined in inflammatory arterial thrombosis. We hypothesized that microbubbles (MBs) target glycoprotein IIb/IIIa (GP IIb/IIIa) could achieve a noninvasive in vivo detection of inflammatory thrombosis in large arteries through contrast-enhanced ultrasound (CEU) imaging.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.