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Find video protocols related to scientific articles indexed in Pubmed.
[Growth activity of osteoblast on a novel strontium incorporated calcium sulfate].
Zhongguo Gu Shang
PUBLISHED: 08-30-2014
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To investigate the growth activity of osteoblast on a novel strontium incorporated calcium sulfate and make comparison with normal calcium sulfate material.
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Evaluation of endoscopic dorsal ramus rhizotomy in managing facetogenic chronic low back pain.
Clin Neurol Neurosurg
PUBLISHED: 08-18-2014
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To study the effectiveness of surgical dorsal endoscopic rhizotomy for the treatment of facetogenic chronic low back pain.
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Low-dose decitabine induces MAGE-A expression and inhibits invasion via suppression of NF-?B2 and MMP2 in Eca109 cells.
Biomed. Pharmacother.
PUBLISHED: 07-18-2014
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Decitabine, a demethylating drug, is the first-line treatment for myelodysplastic syndromes and gains better overall survival, which is based on epigenetic mechanism. Activated by promoter demethylation, melanoma-associated antigens-A (MAGE-A), cancer-testis antigens are attractive targets for immunotherapy. Our purpose was to investigate whether decitabine could show anti-tumor effects for esophageal cancer and explore its mechanism. In addition, we aimed to examine its modulation for most MAGE-A members. The results showed the baseline expression were MAGE-A2, -3,-9, and -10 in Eca109 cells and decitabine (0.5 ?M) could induce MAGE-A8 and -A4 whereas reduce MAGE-A9 and -A10. Moreover, decitabine (0.5 ?M) inhibited cell proliferation, migration and invasive ability by 15%, 34% and 47.2%, respectively and decreased expressions of NF-?B2 and MMP2. Our results demonstrated that low-dose decitabine induced the expression of MAGE-A8 and -A4, and inhibited cell invasion through decreasing expression of MMP2 and NF-?B2, which provides possibilities for combing decitabine with immunotherapy targeting MAGE-A to treat advanced esophageal squamous cell carcinoma.
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Interaction between hydrophobically modified 2-hydroxyethyl cellulose and sodium dodecyl sulfate studied by viscometry and two-dimensional NOE NMR spectroscopy.
J Phys Chem B
PUBLISHED: 06-06-2014
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Interaction between an anionic surfactant, sodium dodecyl sulfate (SDS), and a nonionic polymer, 2-hydroxyethyl cellulose (HEC) hydrophobically modified with benzoyl chloride (bmHEC), is studied by viscometry and two-dimensional nuclear Overhauser effect NMR spectroscopy (2D NOESY) in a semidilute regime of bmHEC. The hydrophobicity of bmHEC was varied with different substitution of benzoyl group to HEC macromolecules. In general, the low-shear viscosity of 1 wt % bmHEC aqueous solution is increased with added SDS surfactant having concentration from 0 to 0.5 wt %, and then decreased significantly with a further addition of surfactant to 3 wt %. The activation energy of transient network formation in 1 wt % bmHEC aqueous solution present with SDS surfactant is found to be dependent with SDS concentration, which varies from 32.7 to 69.80 kJ/mol. The maximum activation energy takes place when 0.5 wt % SDS is added, which coincides with that of the maximal viscosity. The 2D NOESY displays that the surfactants actually interact with bmHEC not only on the hydrophobes, namely benzoyl groups, but also the polymer backbone, i.e., glucose units. In contrast, no interaction is revealed by 2D NOESY in the aqueous system containing SDS surfactant and HEC polymer.
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[Effect of electroacupuncture intervention on enteric microecology in ulcerative colitis rats].
Zhen Ci Yan Jiu
PUBLISHED: 04-02-2014
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To observe the effect of electroacupuncture (EA) on enteric microbial populations in ulcerative colitis (UC) model rats, so as to study its mechanisms underlying improving UC.
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[Improving the accuracy of caregiver-provided in-home total parenteral nutrition care].
Hu Li Za Zhi
PUBLISHED: 04-01-2014
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According to current surveys conducted between September and December 2011, the accuracy of primary caregiver-administered Home Total Parenteral Nutrition Care (HTPNC) was 62.0%. Further, nursing staffs provide instruction on HTPNC to only 22.2% of caregivers responsible for administering HTPNC. Main related causes were: (1) difficult to comprehend health education tools; (2) inconsistent nursing guidelines; (3) a lack of relevant standard operating procedures; and (4) poor caregiver adoption of TPN skills.
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A coumarin-based fluorescent probe for selective and sensitive detection of thiophenols and its application.
Anal. Chem.
PUBLISHED: 03-03-2014
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The development of probes for specific thiophenol detection is of great importance, due to the toxicity of thiophenols and their derivatives in the environment. In the present study, a novel fluorescent probe was rationally designed for detecting thiophenols via an intramolecular charge transfer mechanism. The developed probe selectively and sensitively distinguished thiophenols from aliphatic thiols. It displayed a large Stokes shift (145 nm) and >280-fold fluorescence enhancement. Moreover, the new probe not only displayed excellent cell permeability for the successful detection of thiophenol in HEK293 cells but also quantitatively measured thiophenols in water samples with good recovery (more than 90%), indicating that it has promising prospects for application for thiophenol sensing in environmental and biological sciences.
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High performance of transferring lithium ion for polyacrylonitrile-interpenetrating crosslinked polyoxyethylene network as gel polymer electrolyte.
ACS Appl Mater Interfaces
PUBLISHED: 02-21-2014
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A polyacrylonitrile (PAN)-interpenetrating cross-linked polyoxyethylene (PEO) network (named XANE) was synthesized acting as separator and as gel polymer electrolytes simultaneously. SEM images show that the surface of the XANE membrane is nonporous, comparing to the surface of the commercial separator to be porous. This property results in excellent electrolyte uptake amount (425 wt %), and electrolyte retention for XANE membrane, significantly higher than that of commercial separator (200 wt %). The DSC result indicates that the PEO crystallinity is deteriorated by the cross-linked process and was further degraded by the interpenetration of the PAN. The XANE membrane shows significantly higher ionic conductivity (1.06-8.21 mS cm(-1)) than that of the commercial Celgard M824 separator (0.45-0.90 mS cm(-1)) ascribed to the high electrolyte retention ability of XANE (from TGA), the deteriorated PEO crystallinity (from DSC) and the good compatibility between XANE and electrode (from measuring the interfacial-resistance). For battery application, under all charge/discharge rates (from 0.1 to 3 C), the specific half-cell capacities of the cell composed of the XANE membrane are all higher than those of the aforementioned commercial separator. More specifically, the cell composed of the XANE membrane has excellent cycling stability, that is, the half-cell composed of the XANE membrane still exhibited more than 97% columbic efficiency after 100 cycles at 1 C. The above-mentioned advantageous properties and performances of the XANE membrane allow it to act as both an ionic conductor as well as a separator, so as to work as separator-free gel polymer electrolytes.
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Genetic ablation of receptor for advanced glycation end products promotes functional recovery in mouse model of spinal cord injury.
Mol. Cell. Biochem.
PUBLISHED: 01-21-2014
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Spinal cord injury (SCI) results in a loss of normal motor and sensory function, leading to severe disability and reduced quality of life. The aim of this work was to investigate the effect of receptor for advanced glycation end products (RAGE) deficiency on the function recovery in a mouse model of SCI. Mice received a mid-thoracic spinal contusion injury. Upregulation of RAGE protein expression in spinal cord tissue was evident at 12 h after SCI and continued at 2 and 5 days. Furthermore, we showed that locomotor recovery was improved and lesion pathology was reduced after SCI in RAGE-deficient mice. RAGE deficiency in mice attenuated apoptosis after SCI through inhibiting p53/Bax/caspase-3 pathway. RAGE deficiency in mice inhibited inflammation after SCI, marked by reduced myeloperoxidase activity, NF?B nuclear translocation, and TNF-?, IL-1?, and IL-6 mRNA and protein levels. RAGE deficiency in mice exposed to SCI suppressed the upregulation of inducible nitric oxide synthase (iNOS) and gp91-phox and attenuated oxidative and nitrosative stresses, marked by reduced formation of malondialdehyde, reactive oxygen species, peroxynitrite (OONO(-)), and 3-nitrotyrosine. RAGE deficiency in mice exposed to SCI attenuated glial scar at the injury site, marked by decreased expression of glial fibrillary acidic protein. These data indicate that the RAGE plays an important role in the development of SCI and might provide a therapeutic target to promote recovery from SCI.
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Percutaneous resolution of lumbar facet joint cysts as an alternative treatment to surgery: a meta-analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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A comprehensive review of the literature in order to analyze data about the success rate of percutaneous resolution of the lumbar facet joint cysts as a conservative management strategy.
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NMR studies on effects of tetraalkylammonium bromides on micellization of sodium dodecylsulfate.
J Phys Chem B
PUBLISHED: 09-30-2013
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The effects of tetraalkylammonium bromides (TAABs) on the micellization of sodium dodecylsulfate (SDS) are studied using pyrene solubilization and several nuclear magnetic resonance (NMR) techniques. Two-dimensional nuclear Overhauser effect spectroscopy (2D NOESY) experiments confirm that tetraalkylammonium (TAA(+)) ions associate with SDS to form mixed micelles. TAA(+) ions attach to the surface of the mixed micelles and become inserted into the hydrophobic core of the mixed micelles. Because TAA(+) ions appear in the hydrophobic interior of the TAA-SDS mixed micelles, the micropolarity inside the mixed micelles sensed by pyrene might not reflect the true hydrophobicity of the micellar core. Using proton chemical shift analysis, the degree of hydration on the surface of the mixed micelles is determined from the chemical shift change of SDS ?-CH2 protons. The self-diffusion coefficients of SDS and TAA(+) ions in the TAAB/SDS/D2O solutions are measured by using pulse-field gradient NMR, and the fraction of TAA(+) ions associated with the SDS to form the mixed micelles is calculated from the self-diffusion data. Moreover, secondary micelle formation for SDS and TAA(+) ions is observed on the basis of (1)H chemical shift analysis and the self-diffusion data. The 2D NOESY experiments also reveal unusual tumbling behavior of SDS alkyl protons. For Pr4NBr/SDS and Bu4NBr/SDS solutions, positive and negative nuclear Overhauser effects are simultaneously observed among the SDS alkyl protons.
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Hydrogen water consumption prevents osteopenia in ovariectomized rats.
Br. J. Pharmacol.
PUBLISHED: 08-24-2013
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Accumulating evidence indicates an important role of oxidative stress in the progression of osteoporosis. Recently, it was demonstrated that hydrogen gas, as a novel antioxidant, could selectively reduce hydroxyl radicals and peroxynitrite anion to exert potent therapeutic antioxidant activity. The aim of the present work was to investigate the effect of hydrogen water (HW) consumption on ovariectomy-induced osteoporosis.
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Poly(ethylene oxide)-co-poly(propylene oxide)-based gel electrolyte with high ionic conductivity and mechanical integrity for lithium-ion batteries.
ACS Appl Mater Interfaces
PUBLISHED: 08-22-2013
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Using gel polymer electrolytes (GPEs) for lithium-ion batteries usually encounters the drawback of poor mechanical integrity of the GPEs. This study demonstrates the outstanding performance of a GPE consisting of a commercial membrane (Celgard) incorporated with a poly(ethylene oxide)-co-poly(propylene oxide) copolymer (P(EO-co-PO)) swelled by a liquid electrolyte (LE) of 1 M LiPF6 in carbonate solvents. The proposed GPE stably holds LE with an amount that is three times that of the Celgard-P(EO-co-PO) composite. This GPE has a higher ionic conductivity (2.8×10(-3) and 5.1×10(-4) S cm(-1) at 30 and -20 °C, respectively) and a wider electrochemical voltage range (5.1 V) than the LE-swelled Celgard because of the strong ion-solvation power of P(EO-co-PO). The active ion-solvation role of P(EO-co-PO) also suppresses the formation of the solid-electrolyte interphase layer. When assembling the GPE in a Li/LiFePO4 battery, the P(EO-co-PO) network hinders anionic transport, producing a high Li+ transference number of 0.5 and decreased the polarization overpotential. The Li/GPE/LiFePO4 battery delivers a discharge capacity of 156-135 mAh g(-1) between 0.1 and 1 C-rates, which is approximately 5% higher than that of the Li/LE/LiFePO4 battery. The IR drop of the Li/GPE/LiFePO4 battery was 44% smaller than that of the Li/LE/LiFePO4. The Li/GPE/LiFePO4 battery is more stable, with only a 1.2% capacity decay for 150 galvanostatic charge-discharge cycles. The advantages of the proposed GPE are its high stability, conductivity, Li+ transference number, and mechanical integrity, which allow for the assembly of GPE-based batteries readily scalable to industrial levels.
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Lauroyl/palmitoyl glycol chitosan gels enhance skin delivery of magnesium ascorbyl phosphate.
J Cosmet Sci
PUBLISHED: 08-13-2013
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Palmitoyl glycol chitosan (GCP) hydrogel has been reported as erodible controlled-release systems for the delivery of both hydrophilic and hydrophobic molecules. In this study we prepared lauroyl/palmitoyl glycol chitosan (GCL/GCP) in gel form and evaluated their application for skin delivery of the hydrophilic compound, magnesium ascorbyl phosphate (MAP), which is widely used in cosmetic formulations. Release of MAP from the polymer gels was significantly decreased with increasing concentration of GCL/GCP in the formulations in comparison with glycol chitosan (GC). In both aqueous and 10% ethanol vehicles, MAP flux was increased 1.58- to 3.96-fold of 1% GC from 1% GCL/GCP. Increase in MAP flux was correlated to the increase in GCL/GCP concentration prepared in 10% ethanol vehicle. GCL/GCP, in either water or 10% ethanol vehicles, increased the skin penetration and skin deposition of MAP in comparison with GC, hydroxypropylmethylcellulose, and carbopol, while sustaining its release from the polymer gels. Both the enhancement in skin penetration/deposition and sustained release of MAP were depended on polymer concentration. Also, with increase in polymer concentration, epidermal to dermal drug deposition ratio tended to increase, which will be beneficial to its activity in the epidermis, such as inhibition of tyrosinase and protection from UV damage. These data suggested both GCL and GCP can be applied as delivery vehicles to improve percutaneous absorption of MAP.
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[Clinical characteristics and prognostic factors of 62 cases with Hodgkin lymphoma].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 08-03-2013
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To investigate the clinical characteristics, pathologic types and prognostic factors of Hodgkin lymphoma (HL).
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[Characteristics of dry matter and potassium accumulation and distribution in potato plant in semi-arid rainfed areas].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 05-28-2013
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In 2010, a field experiment with potato (Solanum tuberosum) cultivar Xindaping was conducted at the Dingxi Extension Center of Gansu Province, Northwest China, aimed to understand the accumulation and distribution patterns of dry matter (DM) and potassium (K) in the organs of potato plant in semi-arid rainfed areas. During the whole growth period of the cultivar, the DM accumulation in root, stem, and leaf all showed a unimodal curve, with the DM accumulation rate being leaf > stem > root, whereas the DM accumulation in whole plant and tuber was an S-curve. The maximum DM accumulation rate of the whole plant was higher than that of the tuber, and appeared 17 days earlier. The distribution of DM in different organs showed two turning points, i.e., during the tuber formation (TF) period and the tuber growth (TG) period. During TF period, the DM accumulation was the greatest in leaf, followed by in tuber. The TF period was also the DM balance period, which occurred 90 days after emergence. Before the DM balance period, the DM accumulation in tuber was lesser than that in root, stem, and leaf, and there was a positive correlation between the DM accumulation in tuber and in root, stem, and leaf. However, after the DM balance period, the DM accumulation in tuber was greater than that in root, stem, and leaf, and the correlation was negative. At seedling stage and in TF period, TG period, starch accumulation period, and maturity period, the DM accumulation in whole plant was 5%, 30%, 60%, 4% , and 1%, while that in tuber was 0,18% , 62 , 18% , and 2%, respectively. In the whole growth period, more than 50% of the DM was formed in TG period. The K concentration was the highest in stem and the lowest in tuber, though the K was mostly concentrated in root before the DM balance period. The K accumulation before the DM balance period was mostly in root, stem, and leaf, with the sequence of stem > leaf > root, but after the DM balance period, the K was mainly allocated in tuber, with >60% of the K accumulated in tuber in maturity period.
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Expressions of MAGE-A9 and MAGE-A11 in Breast Cancer and Their Mechanism of Expression.
Arch. Med. Res.
PUBLISHED: 05-27-2013
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The MAGE gene encodes cancer/testis antigens that are selectively expressed in various types of human neoplasms but not in normal tissues other than testis and placenta. However, the expression pattern of MAGE-A9 and MAGE-A11 in breast cancer patients is still unclear. The purpose of our study is to investigate the expression pattern and mechanism of MAGE-A9 and MAGE-A11 in breast cancer patients.
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[Laminectomy and extraction of nucleus pulposus for treatment of lumbar disc herniation: effect evaluation of over 10-year-followed-up].
Zhongguo Gu Shang
PUBLISHED: 04-27-2013
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To investigate retrospectively the clinical effects and recurrence rate of 143 cases who underwent one level discectomy and followed up more than 10 years. To evaluate the outcome of patients in groups of different operating age and extents of disc herniation, and analyse whether difference exists in each group.
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Treatment with resveratrol attenuates sublesional bone loss in spinal cord-injured rats.
Br. J. Pharmacol.
PUBLISHED: 04-14-2013
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Sublesional osteoporosis predisposes individuals with spinal cord injury (SCI) to an increased risk of low-trauma fracture. The aim of the present work was to investigate the effect of treatment with resveratrol (RES) on sublesional bone loss in spinal cord-injured rats.
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Influence of dental restorative material properties on bond interface reliability: a finite element analysis.
Chin. Med. J.
PUBLISHED: 03-16-2013
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Varieties of restorative materials are widely used in dentistry. The aim of this study is to explore the influence of different dental restorative materials on bond interface reliability.
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[Both the medial and lateral meniscal allograft transplantation following the anterior cruciate ligament reconstruction by arthroscopic surgical technique].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 11-02-2011
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To discuss the minimal invasive arthroscopic surgery technique and clinical results of both the medial and lateral meniscal transplantation following the anterior cruciate ligament reconstruction with double bundles and bone tunnels.
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Determination of the glycation sites of Bacillus anthracis neoglycoconjugate vaccine by MALDI-TOF/TOF-CID-MS/MS and LC-ESI-QqTOF-tandem mass spectrometry.
J Mass Spectrom
PUBLISHED: 10-21-2011
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We present herein an efficient mass spectrometric method for the localization of the glycation sites of a model neoglycoconjugate vaccine formed by a construct of the tetrasaccharide side chain of the Bacillus anthracis exosporium and the protein carrier bovine serum albumin. The glycoconjugate was digested with both trypsin and GluC V8 endoproteinases, and the digests were then analyzed by MALDI-TOF/TOF-CID-MS/MS and nano-LC-ESI-QqTOF-CID-MS/MS. The sequences of the unknown peptides analyzed by MALDI-TOF/TOF-CID-MS/MS, following digestion with the GluC V8 endoproteinase, allowed us to recognize three glycopeptides whose glycation occupancies were, respectively, on Lys 235, Lys 420, and Lys 498. Similarly, the same analysis was performed on the tryptic digests, which permitted us to recognize two glycation sites on Lys 100 and Lys 374. In addition, we have also used LC-ESI-QqTOF-CID-MS/MS analysis for the identification of the tryptic digests. However, this analysis identified a higher number of glycopeptides than would be expected from a glycoconjugate composed of a carbohydrate-protein ratio of 5.4:1, which would have resulted in glycation occupancies of 18 specific sites. This discrepancy was due to the large number of glycoforms formed during the synthetic carbohydrate-spacer-carrier protein conjugation. Likewise, the LC-ESI-QqTOF-MS/MS analysis of the GluC V8 digest also identified 17 different glycation sites on the synthetic glycoconjugate.
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[Quality control on acupuncture treatment].
Zhongguo Zhen Jiu
PUBLISHED: 08-10-2011
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The theory and practice of acupuncture treatment are explored and summarized in this article from the viewpoint of clinical practice. And three major principles of quality control on acupuncture treatment is put forward, namely intensive control according to individuals, which divides patients into six categories based on their body constitutions of extreme sensitive, super sensitive, sensitive, normal, dull and super dull; intensive control according to diseases, which adjusts the intensive of acupuncture treatment according to diseases, stage of diseases and progress of diseases; and intensive control according to practitioners, which regulates acupuncture intensive according to techniques of practitioners, opportunities of treatment (season, temperature and condition of the patients, etc.) as well as treating methods. And it is concluded that appropriate intensive is the key point for the best therapeutic effect of acupuncture treatment.
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Simvastatin protects osteoblast against H2O2-induced oxidative damage via inhibiting the upregulation of Nox4.
Mol. Cell. Biochem.
PUBLISHED: 06-23-2011
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Statins, 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors, have been used clinically as a cholesterol-lowering drug to treat hyperlipidemia. In recent years, accumulating evidence indicates the possible beneficial effect of statins on osteoporosis. However, the underlying molecular mechanism remains to be elucidated. In the present study, we investigated the therapeutic effects of simvastatin on cell viability, apoptosis, and alkaline phosphatase activity in murine osteoblastic MC3T3-E1 cells treated by hydrogen peroxide (H(2)O(2), 100 ?M). It was shown that simvastatin suppressed H(2)O(2)-induced oxidative stress and attenuated H(2)O(2)-induced cell injury including increasing osteoblastic viability, inhibiting apoptosis, and promoting differentiation. Then, we examined the effects of simvastatin (10(-7) M) on Nox1, Nox2, and Nox4 expressions in osteoblastic cells treated by H(2)O(2) (100 ?M). We found that in MC3T3-E1 cells, H(2)O(2)-induced upregulation of Nox4 expression was inhibited by simvastatin, which was restored by farnesyl pyrophosphate (5 ?M) as well as geranylgeranyl pyrophosphate (5 ?M). RNAi approach was used to reduce Nox4 protein levels in osteoblastic cells to explore its biological effects against H(2)O(2)-induced oxidative damage. When Nox4 expression was reduced in osteoblastic cells, H(2)O(2)-induced cell injury was attenuated markedly. We concluded that simvastatin protected osteoblast against H(2)O(2)-induced oxidative damage, at least in part, via inhibiting the upregulation of Nox4.
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Mifepristone in combination with prostaglandins for termination of 10-16 weeks gestation: a systematic review.
Eur. J. Obstet. Gynecol. Reprod. Biol.
PUBLISHED: 05-19-2011
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Medical regimens using mifepristone in combination with prostaglandins have been widely available for women undergoing termination of pregnancy (TOP) at 10-16 weeks gestation in China. We undertook a systematic review to compare different regimens of mifepristone with prostaglandins for TOP at 10-16 weeks gestation.
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[Comparison of the grafting technique in treatment of thoracolumbar burst fractures:transpedicular intracorporeal versus posterolateral].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 03-24-2011
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To retrospectively investigate the outcome of transpedicular intracorporeal grafting and posterolateral grafting in treatment of thoracolumbar burst fractures.
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Termination of second-trimester pregnancy by mifepristone combined with misoprostol versus intra-amniotic injection of ethacridine lactate (Rivanol®): a systematic review of Chinese trials.
Contraception
PUBLISHED: 01-14-2011
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Mifepristone combined with misoprostol for second-trimester abortion (MM) and intra-amniotic injection of ethacridine lactate (EL; Rivanol®) are the common methods for termination of second-trimester pregnancy in China. The systematic review of relevant literature was conducted to evaluate the effectiveness and safety on termination of second-trimester pregnancy using MM, introduced in 1988 in China, versus the Chinese routinely used method since 1970 -- EL. The review was conducted to evaluate mifepristone combined with the misoprostol versus intra-amniotic injection of ethacridine lactate in China for termination of second-trimester pregnancy with respect to efficacy, side effects, complications and so on.
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[The anatomic and radiographic morphometry of thoracic pedicle rib unit].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 11-25-2010
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To investigate the spatial structure of pedicle rib units in normal thoracic human spines and to compare the dimensions of the pedicle rib unit with corresponding dimensions.
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[Classified teaching of acupoints: standardized teaching in Jingluo Shuxue Xue (Channels and Acupoints science)].
Zhongguo Zhen Jiu
PUBLISHED: 11-10-2010
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The purpose of this study is to summarize teaching experiences on channel and acupoints. The method of classified teaching is put forward and approached to explore the law of acupoint teaching. It is held that the method could reduce randomness and enhance appropriateness, rationality and scientific validity of the teaching. Class hours are thus optimized and the best teaching result achieved. Acupoints are classified into 4 categories of core points, focal points, regular points and ordinary points according to their use frequency and theoretical importance. Classification methods, principles and categories are then determined. And content of courses, teaching techniques and requirements are introduced as well. Suggestions are offered on enriching teaching contents, for instance, paraphrasing of the acupoint names, indications, point prescriptions and clinical experiences. Thus the conclusion is made that it is imperative to reform the conventional sort of acupoints classified by only focal points and ordinary points as well as the related teaching methods.
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[Strong association between the NINJ2 gene polymorphism and the susceptibility of stroke in Chinese Han population in Fangshan district].
Beijing Da Xue Xue Bao
PUBLISHED: 10-20-2010
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To investigate the association between the NINJ2 gene rs11833579 polymorphism and stroke in Han Chinese population.
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Frontal function, disability and caregiver burden in elderly patients with major depressive disorder.
Kaohsiung J. Med. Sci.
PUBLISHED: 03-02-2010
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Caregivers of patients with late-life major depressive disorder experience a significant level of general caregiver burden. Disability in patients is possibly one of the origins of caregiver burden. Frontal lobe dysfunction might be the source of disability. This study investigated if frontal lobe dysfunction (body level) of patients with late-life major depressive disorder was associated with their disability (individual level), and if it led to a high level of caregiver burden (societal level). Thirty-four unselected pairs of caregivers and their family members with late-life major depressive disorder were recruited. Frontal Assessment Battery and Timed Instrumental Activities of Daily Living (TIADL) were used to assess patients frontal function and disability, and Caregiver Burden Inventory was used to measure caregiver burden. Frontal Assessment Battery correlated with TIADL (r= -0.47; p<0.006). TIADL score was also associated with two subscales of the Caregiver Burden Inventory: social (r = 0.38, p=0.026) and time-dependent (r= 0.37, p= 0.033). This study supported the hypothesis that frontal lobe dysfunction in elderly patients with depression is associated with their disability in instrumental activities of daily living. Disability is related to social and time-dependent aspects of caregiver burden. Further studies to examine proposed cognitive interventions are suggested to reduce patient disability and caregiver burden.
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Quality of life and its correlates in patients with obsessive-compulsive disorder.
Kaohsiung J. Med. Sci.
PUBLISHED: 03-02-2010
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The care of patients with obsessive-compulsive disorder (OCD) has raised quality of life (QOL) issues. The purpose of this study was to compare the level of QOL between patients with and without OCD, and to examine the associations between QOL and sociodemographic data, course of illness, psychopathology, perceived social support, and treatment characteristics. The QOL levels measured with the Taiwan version of the Short Form of the World Health Organization Questionnaire on Quality of Life were compared between 57 subjects with OCD and 106 subjects without OCD. The correlates of QOL were examined among subjects with OCD. The analysis revealed that QOL scores for the general, physical, psychological and social relationship domains were lower in the OCD group than in the control group; however, no difference in the environmental domain was found. Multiple factors were associated with poor QOL in subjects with OCD, including comorbid depression, severe obsession symptoms, perceived low social support, severe adverse effects of medication, combined use of mood stabilizers, and low social status. Different domains of QOL are differently affected by OCD. The QOL of subjects with OCD was correlated to multiple factors that were specific to individual subjects and influenced by interactions with treatment and the social environment.
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Synthesis and in vitro cytotoxicity of cis-[Pt(NH(3))(NH(2)OH)Cl(2)].
Arch. Pharm. Res.
PUBLISHED: 02-21-2010
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A novel mixed NH(3)/NH(2)OH platinum(II) complex cis-[Pt(NH(3))(NH(2)OH)Cl(2)] was synthesized and characterized by elemental analysis, FAB-MS, FT-IR and (1)H NMR spectroscopy. This complex was determined to have a good water-solubility and satisfactory stability. The pertinent complex was evaluated for its in vitro cytotoxicity against 3AO, HCT-116, LNcap, A549/ATCC and SGC-7901 human carcinoma cell lines. It shows appreciable cytotoxic activity that is comparable with cisplatin and is much more active than carboplatin.
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Enhanced stereoselectivity of alpha-mannosylation under thermodynamic control using trichloroacetimidates.
Carbohydr. Res.
PUBLISHED: 02-04-2010
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O-Specific polysaccharides of Vibrio cholerae O1, serotypes Inaba and Ogawa, consist of alpha-(1-->2)-linked N-(3-deoxy-L-glycero-tetronyl)perosamine (4-amino-4,6-dideoxy-D-mannose). The blockwise synthesis of larger fragments of such O-PSs involves oligosaccharide glycosyl donors that contain a nonparticipating 2-O-glycosyl group at the position vicinal to the anomeric center where the new glycosidic linkage is formed. Such glycosyl donors may bear at C-4 either a latent acylamino (e.g., azido) or the 3-deoxy-L-glycero-tetronamido group. While monosaccharide glycosyl donors, even those bearing a nonparticipating group at O-2 (e.g., methyl), and the 4-N-(3-deoxy-L-glycero-tetronyl) side chain form alpha-linked oligosaccharides with excellent stereoselectivity, alpha-mannosylation with analogous oligosaccharide donors in this series is adversely affected by the presence of the side chain. Consequently, the unwanted beta-product is formed in a considerable amount. Conducting the reaction at elevated temperature under thermodynamic control substantially enhances formation of the alpha-linked oligosaccharide. This effect is much more pronounced when glycosyl trichloroacetimidates, rather than thioglycosides or glycosyl chlorides, are used as glycosyl donors.
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[The application of external fixator for complex tissue defect in the forearm].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 12-05-2009
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To discuss the curative effect of the external fixator for complex tissue defect in the forearm.
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[The application of relaxation techniques and biofeedback to an outpatient with depression disorder: a nurses experience].
Hu Li Za Zhi
PUBLISHED: 12-03-2009
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This paper reports on a nurses experience helping alleviate psychosomatic symptoms in a psychiatric outpatient suffering from depression. It was found that the OPD patient had been suffering from depression as well as long-time tension and pain. Between April 10th and July 24th, 2008, the author employed holistic nursing assessment and collected data through both observation and interview. Three major care problems were identified, including ineffective individual coping, chronic pain, and sleep disturbance. Focusing nursing care on relaxation techniques and biofeedback, the author discussed the problems with the patient and then implemented relaxation training and biofeedback training sessions as well as nursing care plans, which averaged 45~60 minutes per time for 8 times. Sessions included interviews, relaxation techniques (e.g., progressive muscular relaxation, meditative relaxation, meditation, music and abdominal breathing) and visual feedback measured by a biofeedback device with a thermistor sensor. During sessions, the patient was clearly motivated to change and participated actively. Furthermore, the patient not only became gradually aware of both the inner feeling of relaxation and etiology of psychosomatic symptoms, but also learned relaxation strategies for coping with life stressors. After therapy, the patients self-control over relaxation had improved considerably and care problems were resolved satisfactorily. Also, the patient could increasingly incorporate relaxation into daily life routines.
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[Discussion on the treatment amount of acupuncture and moxibustion].
Zhongguo Zhen Jiu
PUBLISHED: 12-02-2009
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In the present paper, the theory and practice of acupuncture and moxibustion therapy by a clinical point of view are explored and summarized. Such concepts as the total treatment amount, the unit treatment amount, the immediate treatment amount, the overtreatment amount, the inferior treatment amount and the measurement of treatment amount are proposed and discussed. The basic constituent elements of treatment amount of acupuncture and moxibustion, i. e. acupuncture, moxibustion, cupping, acupoint injection, electroacupuncture are summarized. It is held that the treatment amount of acupuncture and moxibustion in condition of suitable controlling is the fundamental guarantee for the best clinical therapeutic effect, the key to which include correct acupuncture manipulation, rational selection acupoints for the prescriptions, suitable acupuncture therapy combination, the variety of treatment amount assignment being related with the state of disease and so on. The overtreatment amount and the inferior treatment amount must be avoided.
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Genetics of osteoporosis: accelerating pace in gene identification and validation.
Hum. Genet.
PUBLISHED: 10-27-2009
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Osteoporosis is characterized by low bone mineral density and structural deterioration of bone tissue, leading to an increased risk of fractures. It is the most common metabolic bone disorder worldwide, affecting one in three women and one in eight men over the age of 50. In the past 15 years, a large number of genes have been reported as being associated with osteoporosis. However, only in the past 4 years we have witnessed an accelerated pace in identifying and validating osteoporosis susceptibility loci. This increase in pace is mostly due to large-scale association studies, meta-analyses, and genome-wide association studies of both single nucleotide polymorphisms and copy number variations. A comprehensive review of these developments revealed that, to date, at least 15 genes (VDR, ESR1, ESR2, LRP5, LRP4, SOST, GRP177, OPG, RANK, RANKL, COLIA1, SPP1, ITGA1, SP7, and SOX6) can be reasonably assigned as confirmed osteoporosis susceptibility genes, whereas, another >30 genes are promising candidate genes. Notably, confirmed and promising genes are clustered in three biological pathways, the estrogen endocrine pathway, the Wnt/beta-catenin signaling pathway, and the RANKL/RANK/OPG pathway. New biological pathways will certainly emerge when more osteoporosis genes are identified and validated. These genetic findings may provide new routes toward improved therapeutic and preventive interventions of this complex disease.
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[Studying the influence of age and short or long segments of pedicle screw instrumentation to the clinical efficacy of early single thoracolumbar fracture].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 10-19-2009
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To study the influence of different age and short or long segments of pedicle screw fixation to the clinical efficacy of early single thoracolumbar fracture.
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[The role of capsaicin-sensitive primary afferents in experimental sciatic pain].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 09-29-2009
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To determine the effect of destroying capsaicin-sensitive primary afferents (CSPA) fibers on paw withdrawal mechanical threshold (PWMT) induced by the direct compression of L5 nerve root with autologous disc.
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Bacterial community in the East Rongbuk Glacier, Mt. Qomolangma (Everest) by culture and culture-independent methods.
Microbiol. Res.
PUBLISHED: 07-25-2009
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The abundance of total and culturable bacteria deposited into the East Rongbuk ice core was investigated, and the bacterial content was examined through culture and culture-independent approaches. Total counts of bacteria in the ice core ranged from 0.02 x 10(3) to 6.4 x 10(3) cells ml(-1). Viable bacteria varied between 0 and 5.6 CFU ml(-1) on PYGV and R2A media. The phylogenetic trees placed the culturable bacteria into four major groups: Actinobacteria, Firmicutes, Proteobacteria and Deinococcus-Thermus, with Firmicutes being the most prevalent. They exhibited unique phenotypic properties with large and non-pigmented cells. The diversity revealed by H index of DGGE analysis was 0-0.75, and closer sections showed higher similarity of bacterial DNA structure. Members of two major lineages were found: Firmicutes and Proteobacteria. The combined culture and culture-independent methods indicated layer distribution of bacterial community in the ice core section, which might reflect the ecological environments on glacier at time of their deposition.
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[Effect of icogenin on and its mechanism in anti-metastasis of pancreatic cancer BxPC3 cells].
Yao Xue Xue Bao
PUBLISHED: 07-22-2009
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This study is to investigate the effect of Icogenin on and its mechanism in anti-metastasis of pancreatic cancer BxPC3 cells in vitro. Using transwell assay, the effects of Icogenin on the invasion of BxPC3 cells were measured. The abilities of cell motility and adhesion in BxPC3 cells were detected by MTT assay and wound healing assay, respectively. The MAPK signal pathway protein expressions were analyzed with Western blotting. Also, the activity of MMP2 was observed by zymography assay. Icogenin inhibited the abilities of motility, adhesion and invasion of pancreatic cancer BxPC3 cells in vitro (P < 0.05), in a dose-depended manner, and inhibited the secretion of MMP2 and phosphorylation of ERK. PD98059 and U0126 which were ERK inhibitors could suppress the abilities of invasion and metastasis of pancreatic cancer BxPC3 cells. It is concluded that Icogenin can inhibit the abilities of invasion and metastasis of pancreatic cancer in vitro by inhibiting the secretion of MMP2 and phosphorylation of ERK.
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[Transplantation of large anterior latissimus dorsi muscular flap combination with musculus rectus abdominis flap].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 07-15-2009
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To study the value and surgical techniques of transplantation of large anterior latissimus dorsi muscular flap combination with musculus rectus abdominis flap.
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Synthesis, characterization and cytotoxicity of dihalogeno-platinum(II) complexes with L-histidine ligand.
Chem. Pharm. Bull.
PUBLISHED: 04-02-2009
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Diiodo-, dibromo- and dichloro-platinum(II) complexes containing L-histidine ligand were prepared. Their spectra and X-ray crystal structure of the dibromo-platinum(II) complex were described. Only the dichloro-platinum(II) complex showed comparable cytotoxic activity with carboplatin against A549/ATCC, HT-29, and LNcap cell lines. Nevertheless the complexes with COOH-substituted ligands histidine may be good starting materials to synthesize targeting platinum complexes since they could be easily linked to suitable carrier molecules via esterification.
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Fluconazole-resistant Kodamaea ohmeri fungemia associated with cellulitis: case report and review of the literature.
Int. J. Infect. Dis.
PUBLISHED: 01-18-2009
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Kodamaea (Pichia) ohmeri was formerly considered a contaminant, but is now known to be a significant human pathogen that has been shown to cause fungemia, endocarditis, funguria, and peritonitis in immunocompromised patients. We report a case of fungemia caused by K. ohmeri in a 71-year-old man with cellulitis. The patient was sent to the emergency room due to leg edema, fever, and change of consciousness. During hospitalization, a series of examinations including blood cultures were performed. On hospital day 8, blood culture yielded a yeast colony. Fluconazole was given empirically, but had no effect. The pathogen was identified as K. ohmeri by Vitek YBC card, API 20C, sequencing of the 18S rRNA gene, and the D1/D2 domains of the 26S rRNA gene and the internally transcribed spacer (ITS) regions. Antifungal susceptibility testing was performed with the ATB-Fungus system, and a high minimum inhibitory concentration (level up to 64 mg/l) for fluconazole was found. Fluconazole was replaced with amphotericin B deoxylate, and the fever and cellulitis inflammation gradually subsided. The patient was discharged in a stable condition. This is the first case of K. ohmeri fungemia in Taiwan.
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[Experimental study on fresh meniscal allografts combined with osteochondral allografts transplantation].
Zhongguo Gu Shang
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To investigate the effect of fresh meniscal allografts combined with osteochondral allografts transplantation for treatment of osteoarthritis.
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[Surgical treatment of thoracolumbar single compression fracture by using in situ rod rotation reduction].
Zhongguo Gu Shang
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To evaluate the efficacy and clinical outcome of the treatment of thoracolumbar single compression fracture by using in situ rod rotation reduction and short segment pedicle screw at the fracture level.
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[Discussion on the control of treatment amount of acupuncture and moxibustion for peripheral facial paralysis].
Zhongguo Zhen Jiu
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To explore the control principles of treatment amount of acupuncture and moxibustion for peripheral facial paralysis. (1) Early stage: in this period, the selected acupoints should be few, therapy should be easy and simple, and the treatment amount should be small and basically constant, which is called constant acupoints and amount. (2) Middle stage: the treatment of this stage should be given with more acupoints and various therapies, the treatment amount should be gradually increasing and reach the peak within a certain time and keep it for an appropriate time, which is called increasing acuponts and amount. (3) Late stage: the treatment at this stage should be given with more acupoints, lesser therapies, and the treatment amount should be gradually decreasding and get the valley point within a certain time, which is called more acupoints and less amount. (4) Sequelae stage: the selection of acupoints at this stage should be focus on areas which there are the obvious symptoms, and solo type of therapy and little treatment amount is required, which is called changing acupoints and little amount. In a word, the best therapeutic effect could be achieved on condition that the control principles of treatment amount for peripheral facial paralysis are followed during the clinical practice.
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[Measurement of diameter of T4 pedicle-rib compomers].
Zhongguo Gu Shang
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To measure the diameter of T4 pedicle-rib compomers in normal human spines and discuss the importance of related dates.
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Nurse staffing levels make a difference on patient outcomes: a multisite study in Chinese hospitals.
J Nurs Scholarsh
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The purpose of this study is to examine the relationship between nurse staffing and patient outcomes in hospitals in mainland China.
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Paroxysmal supraventricular tachycardia in an infant with hand, foot, and mouth disease.
Ann Dermatol
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An 11-month-old male infant was admitted to our hospital with fever, fussiness, poor feeding, vomiting, and tachypnea for two days prior. Physical examination revealed sporadic papules and vesicles occurring on his hands, feet, face, and perianal mucosa. Enterovirus 71 was identified from both throat swab and vesicle fluid using virus isolation techniques. The patients heart rate fluctuated in a very narrow range from 180~210/beats/min regardless of his physiologic state. An electrocardiogram showed P-waves buried within or occurring just after regular, narrow, QRS complexes. The patient was diagnosed as having hand, foot, and mouth disease in combination with paroxysmal supraventricular tachycardia (PSVT). The child recovered well with symptomatic treatment, including intravenous administration of acyclovir, glucocorticoids, immunoglobulin, adenosine, and sotalol. PSVT was terminated within 36 hours of hospitalization. The skin lesions became crusted on the third day, and then proceeded to heal spontaneously. Here we report on this unusual case and review the associated literature.
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Arthroscopic combined medial and lateral meniscus transplantation after double-tunnel, double-bundle anterior cruciate ligament reconstruction in the same knee.
Knee
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Meniscus transplantation in combination with anterior cruciate ligament (ACL) reconstruction has been used in the treatment of patients with meniscus and ACL deficiency. However, there have been no reports of arthroscopic surgery and the outcome of both medial and lateral meniscus allograft transplantation after double-tunnel, double-bundle ACL reconstruction. Herein, we report the case of a young male who received arthroscopic lateral and medial meniscectomy and ACL tibialis allograft reconstruction performed with the double-tunnel and double-bundle technique approximately 8 months after a knee injury. Approximately 4 months postoperatively he began to experience pain and weakness in the operated knee and subsequently underwent arthroscopic lateral and medial meniscus allograft transplantation in the same procedure. Second-look arthroscopy and magnetic resonance imaging revealed the meniscal allografts to have normal shape and the ACL grafts to be relatively intact at 18 and 30 months after surgery. His knee appeared stable and the range of motion was normal. Our hypothesis was that knee stability could reliably be restored with this combined procedure and the meniscal grafts and ACL graft could provide protection for each other. We suggest that medial and lateral meniscus allografts for one patient should be from the same donor. In the operation, attention must be paid to the direction of the bone tunnels used to fix the horns of the meniscal grafts to avoid communication with other tunnels in the tibial plateau.
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[Clinical safety about repairing the peripheral nerve defects with chemically extracted acellular nerve allograft].
Zhonghua Wai Ke Za Zhi
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To discuss the clinical safety about repairing the peripheral nerve defects with the acellular allogeneic nerve.
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Sirt1 overexpression protects murine osteoblasts against TNF-?-induced injury in vitro by suppressing the NF-?B signaling pathway.
Acta Pharmacol. Sin.
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Sirtuin 1 (Sirt1) is the class III histone/protein deacetylase that interferes with the NF-?B signaling pathway, thereby has anti-inflammatory function. This study was undertaken to investigate whether Sirt1 could protect osteoblasts against TNF-?-induced injury in vitro.
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Determination of glycation sites by tandem mass spectrometry in a synthetic lactose-bovine serum albumin conjugate, a vaccine model prepared by dialkyl squarate chemistry.
Rapid Commun. Mass Spectrom.
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Neoglycoconjugate vaccines synthesized by the squaric acid spacer method allow single point attachment of the carbohydrate antigen to the protein carrier. However, the localization of the carbohydrate antigen sites of conjugation on the protein carrier has been an elusive task difficult to achieve.
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Changes in T lymphocyte subsets and intracellular cytokines after transfer of chemically extracted acellular nerve allografts.
Mol Med Rep
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The aim of the present study was to observe the immune mechanism underlying the rejection of chemically extracted acellular nerve allografts for use in clinical applications. A total of 128 BALB/c mice were randomly divided into a negative contrast group (NC, 32 mice), a fresh autograft group (AG, 32 mice), a fresh allogeneic nerve group (FN, 32 mice) and a chemically extracted acellular allogeneic nerve group (CEN, 32 mice). Various types of nerve grafts were implanted into the thigh muscle of BALB/C mice in the corresponding groups. At 3, 7, 14 and 28 days post-operation, the mice (8 cases from each group) were sacrificed and their spleens were extracted. The spleens were ground into paste. The erythrocytes and other cells were lysed using distilled water and the T lymphocytes were collected. Monoclonal antibodies (CD3, CD4, CD8, CD25, IL-2, IFN-? and TNF-?) were then added to the solution. The Facial Action Coding System was used to determine the positive rates of the cells combined with the monoclonal antibodies above. No significant statistical differences were observed between the CEN, NC and AG groups. However, some data of the FN group were significantly higher than those of the other groups at the corresponding time. No obvious immune rejections were observed among the chemically extracted acellular nerve allografts compared with fresh nerve autograft.
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