JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Evaluation of Plasma Exchange and Continuous Veno-Venous Hemofiltration for the Treatment of Severe Avian Influenza A (H7N9): A Cohort Study.
Ther Apher Dial
PUBLISHED: 11-04-2014
Show Abstract
Hide Abstract
Avian influenza A (H7N9) is a severe disease with high mortality. Hypercytokinemia is thought to play an important role in the pathogenesis. This study was to investigate the efficiency of plasma exchange (PE)?+?continuous veno-venous hemofiltration (CVVH) on the removal of inflammatory mediators and their benefits in the management of fluid overload and metabolic disturbance. In total, 40 H7N9-infected patients were admitted to our hospital. Sixteen critically ill H7N9-infected patients received combination of PE and CVVH. Data from these 16 patients were collected and analyzed. The effects of PE?+?CVVH on plasma cytokine/chemokine levels and clinical outcomes were examined. H7N9-infected patients had increased plasma levels compared to healthy controls. After 3?h of PE?+?CVVH treatment, the cytokine/chemokine levels descended remarkably to lower levels and were maintained thereafter. PE?+?CVVH also benefited the management of fluid, cardiovascular dysfunction and metabolic disturbance. Of the 16 critically ill patients who received PE?+?CVVH, 10 patients survived. PE?+?CVVH decreased the plasma cytokine/chemokine levels significantly. PE?+?CVVH were also beneficial to the management of severe avian influenza A (H7N9).
Related JoVE Video
The Survey on Implementation of Evidence-Based Nursing in Preventing Ventilator-Associated Pneumonia and the Effect Observation.
Cell Biochem. Biophys.
PUBLISHED: 10-07-2014
Show Abstract
Hide Abstract
The objective is to survey the effect of evidence-based nursing (EBN) in preventing ventilator-associated pneumonia and to observe the effect, providing a reference for clinical nursing intervention. 146 patients with mechanical ventilator were included and randomly divided into control group (n = 73) and observation group (n = 73). Patients in control group received conventional nursing method, and patients in observation group received EBN according to the nursing principles. The questions were raised and the literatures were retrieved to formulate the optimum nursing strategy according to clinical experience and patient need. After nursing, morbidity of VAP in observation group was significantly lower than control group, and length of stay, length of stay in ICU, and the duration of mechanical ventilation were also significantly lower than control group; the differences were statistically significant (P < 0.05). After nursing, the physiological function, physical role, somatic pain, and mental health scores in observation group were significantly higher than control group (P < 0.05). Oral hygiene status in observation group was significantly better than control group; the difference was statistically significant (P < 0.05). After nursing, IL-1, IL-6, and TNF-? in two groups were both decreased (P < 0.05); IL-1, IL-6 and TNF-? in observation group were significantly lower than control group (P < 0.05). When patients were discharged, satisfaction degree in observation group was significantly higher than control group; the difference was statistically significant (P < 0.05). The implementation of EBN can effectively prevent the occurrence of ventilator-associated pneumonia and improve the clinical nursing quality, which is worthy of clinical application.
Related JoVE Video
Gender differences in associations of serum ferritin and diabetes, metabolic syndrome, and obesity in the China Health and Nutrition Survey.
Mol Nutr Food Res
PUBLISHED: 09-29-2014
Show Abstract
Hide Abstract
This study examines gender differences in associations of serum ferritin and diabetes, metabolic syndrome (MetS), and obesity in Chinese.
Related JoVE Video
The association between internet and television access and disordered eating in a Chinese sample.
Int J Eat Disord
PUBLISHED: 09-14-2014
Show Abstract
Hide Abstract
China has historically reported a low prevalence of eating disorders. However, the rapid social and economic development of this country as well as Western ideals widely disseminated by television and the Internet have led to distinct patterns of behavioral choices that could affect eating disorder risk. Thus, the current study explored the relation between disordered eating and media use.
Related JoVE Video
Prevalence of Screening-Detected Eating Disorders in Chinese Females and Exploratory Associations with Dietary Practices.
Eur Eat Disord Rev
PUBLISHED: 08-21-2014
Show Abstract
Hide Abstract
China is undergoing dramatic Westernization, hence may be able to provide unique insights into the role of sociocultural factors in disease. The purpose of this exploratory study was two-fold: to describe the prevalence of screening-detected eating disorders and disordered eating in China at the first occasion of assessment in the large-scale China Health and Nutrition Survey (CHNS) and to explore the associations between dietary practices and disordered eating. Regarding the first objective, participants are provincially representative and in subsequent waves will be followed longitudinally.
Related JoVE Video
Oolong tea drinking could help prevent bone loss in postmenopausal Han Chinese women.
Cell Biochem. Biophys.
PUBLISHED: 07-04-2014
Show Abstract
Hide Abstract
The aim of this study was to analyze the relationship between oolong tea drinking and bone mineral density in postmenopausal Han Chinese women, while living and diet habits, fertility, disease elements and other baseline conditions were controlled. One group included 124 cases who routinely drank oolong tea, and the other included 556 who did not drink tea. Data were collected on participant age, lifestyle habits, fertility condition, disease elements, and lumbar, and hip bone densities. It was found that the bone densities of the greater trochanteric bone in tea drinkers were higher (0.793 ± 0.119 kg/cm(2)) than that in non-tea drinkers (0.759 ± 0.116 kg/cm(2), F = 6.248, p = 0.013). Similarly, the bone density of Ward's triangular bone in tea drinkers was higher (0.668 ± 0.133 kg/cm(2)) than that in non-tea drinkers (0.637 ± 0.135 kg/cm(2), F = 6.152, p = 0.013). Oolong tea drinking could help prevent bone loss in postmenopausal Chinese women.
Related JoVE Video
Combination Therapy With Sorafenib and Radiofrequency Ablation for BCLC Stage 0-B1 Hepatocellular Carcinoma: A Multicenter Retrospective Cohort Study.
Am. J. Gastroenterol.
PUBLISHED: 05-01-2014
Show Abstract
Hide Abstract
OBJECTIVES:The objective of this study was to evaluate the efficacy of combined therapy using Sorafenib and radiofrequency ablation (RFA) with curative intent for all detectable lesions in patients with Barcelona Clinic Liver Cancer (BCLC) Stage 0-B1 hepatocellular carcinoma (HCC).METHODS:One hundred and twenty-eight patients with HCC from 12 centers were enrolled in this retrospective study; 64 patients who received Sorafenib plus RFA (Sorafenib-RFA) were compared with a control group treated with RFA alone. The two patient groups were selected with a predefined criterion and matched in terms of their clinical and tumor characteristics at baseline. The primary end point of the study was the incidence of post-RFA HCC recurrence. Secondary end points were overall survival (OS) and treatment toxicity.RESULTS:During a median follow-up of 134.1 weeks, 49 patients died and 79 survived. The 1-, 2-, and 3-year cumulative incidences of post-RFA recurrence were 40.5%, 62.9%, and 74.5%, respectively, in the Sorafenib-RFA group, and 62.8%, 85.4%, and 92.7%, respectively, in the RFA group. The 1-, 2-, 3-, and 4-year OS rates were 85.6%, 64.0%, 58.7%, and 50.3%, respectively, in the Sorafenib-RFA group, and 80.7%, 47.2%, 30.9%, and 30.9%, respectively, in the RFA group. Thus, the Sorafenib-RFA group exhibited better survival than the RFA alone group.CONCLUSIONS:Combined therapy with Sorafenib-RFA was associated with a lower incidence of post-RFA recurrence and better OS than RFA alone in patients with BCLC Stage 0-B1 HCC. Although these findings suggest that Sorafenib and RFA is safe and effective for the treatment of early HCC, prospective and randomized controlled trials are needed to validate them.Am J Gastroenterol advance online publication, 18 November 2014; doi:10.1038/ajg.2014.343.
Related JoVE Video
Trends in incidence of hypertension in Chinese adults, 1991-2009: the China Health and Nutrition Survey.
Int. J. Cardiol.
PUBLISHED: 04-24-2014
Show Abstract
Hide Abstract
Previous studies have shown an upward trend in the prevalence of hypertension, but data on trend of incidence of hypertension are lacking. We seek to investigate the trends in incidence of hypertension and control of incident hypertension among Chinese adults during 1991-1997 and 2004-2009.
Related JoVE Video
Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay.
BMC Infect. Dis.
PUBLISHED: 03-18-2014
Show Abstract
Hide Abstract
A novel avian influenza A (H7N9) virus emerged in eastern China in February 2013. 413 confirmed human cases, including 157 deaths, have been recorded as of July 31, 2014.
Related JoVE Video
Increased frequency of circulating follicular helper T cells in children with hand, foot, and mouth disease caused by enterovirus 71 infection.
J Immunol Res
PUBLISHED: 03-08-2014
Show Abstract
Hide Abstract
Enterovirus 71 (EV71) is a major causative agent of hand, foot, and mouth disease (HFMD) in children. The role of T follicular helper (TFH) cells in EV71-infected children remains unclear in regulating humoral immunity. The frequency of circulating ICOS(high)/PD-1(high)CXCR5(+)CD4(+) TFH cells in the children with mild and severe EV71 infection and healthy controls (HC) was detected by flow cytometry, respectively. IL-21 and IL-6 mRNA expression and their serum levels, Bcl-6 mRNA expression, and specific neutralizing antibodies against EV71 (NAb-EV71) were measured. In the acute stage of patients with EV71 infection, increased frequencies of circulating TFH cells with ICOS(high) and PD-1(high) expression in the mild and severe patients were observed, and the positive correlations among the frequencies of circulating TFH cells and the serum levels of IL-21, IL-6, and NAb-EV71 titres were detected, respectively. Moreover, the expressions of IL-6 and IL-21 mRNA in PBMCs from patients were also significantly higher than those of HC. However, further analysis did not reveal any significant differences between mild and severe patients. These data indicate a role of TFH cells and associated cytokines in modulating the humoral response during the pathogenesis of EV71 infection.
Related JoVE Video
Angiotensin II plasma levels are linked to disease severity and predict fatal outcomes in H7N9-infected patients.
Nat Commun
PUBLISHED: 03-07-2014
Show Abstract
Hide Abstract
A novel influenza A (H7N9) virus of avian origin emerged in eastern China in the spring of 2013. This virus causes severe disease in humans, including acute and often lethal respiratory failure. As of January 2014, 275 cases of H7N9-infected patients had been reported, highlighting the urgency of identifying biomarkers for predicting disease severity and fatal outcomes. Here, we show that plasma levels of angiotensin II, a major regulatory peptide of the renin-angiotensin system, are markedly elevated in H7N9 patients and are associated with disease progression. Moreover, the sustained high levels of angiotensin II in these patients are strongly correlated with mortality. The predictive value of angiotensin II is higher than that of C-reactive protein and some clinical parameters such as the PaO2/FiO2 ratio (partial pressure of arterial oxygen to the fraction of inspired oxygen). Our findings indicate that angiotensin II is a biomarker for lethality in flu infections.
Related JoVE Video
Dynamic behavior of lymphocyte subgroups correlates with clinical outcomes in human H7N9 infection.
J. Infect.
PUBLISHED: 02-17-2014
Show Abstract
Hide Abstract
To investigate peripheral blood lymphocyte subgroups response to the H7N9 virus and identify potential correlations between the anti-viral response and clinical outcomes in infected patients.
Related JoVE Video
Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants.
Chemosphere
PUBLISHED: 01-24-2014
Show Abstract
Hide Abstract
REMI (restriction enzyme-mediated integration) technique was employed to construct Trichoderma atroviride strain T23 mutants with degrading capability of neonicotinoid insecticide, imidacloprid. The plasmid pBluescript II KS-hph used for integration in REMI mutants was confirmed by PCR and Southern hybridization. Among 153 transformants, 57% of them have showed higher neonicotinoid insecticide, imidacloprid, degradation ability than the wild strain T23 (p<0.01). More specifically, seven single-copied T. atroviride T23 transformants have confirmed a 30% higher degradation rate than their parent isolate. Among all transformed mutants, a 95% imidacloprid degradation rate was identified as the highest. This study, thus, provided an effective approach for improving neonicotinoid insecticide-degrading capability using REMI transformed T. atroviride mutants.
Related JoVE Video
Understanding the patterns and trends of sodium intake, potassium intake, and sodium to potassium ratio and their effect on hypertension in China.
Am. J. Clin. Nutr.
PUBLISHED: 11-20-2013
Show Abstract
Hide Abstract
Recent studies have shown inconsistent effects of sodium reduction, potassium intake, and the ratio of sodium to potassium (Na/K ratio) on hypertension and other cardiovascular diseases. Major gaps exist in knowledge regarding these issues in China.
Related JoVE Video
Pathogenic and obesogenic factors associated with inflammation in Chinese children, adolescents and adults.
Am. J. Hum. Biol.
PUBLISHED: 08-13-2013
Show Abstract
Hide Abstract
Influenced by pathogen exposure and obesity, inflammation provides a critical biological pathway linking changing environments to the development of cardiometabolic disease. This study tests the relative contribution of obesogenic and pathogenic factors to moderate and acute CRP elevations in Chinese children, adolescents and adults.
Related JoVE Video
Avian-Origin Influenza A(H7N9) Infection in Influenza A(H7N9)-Affected Areas of China: A Serological Study.
J. Infect. Dis.
PUBLISHED: 08-09-2013
Show Abstract
Hide Abstract
Serological surveillance conducted in areas of an outbreak of influenza A(H7N9) infection in China found no seropositivity for antibodies specific for avian-origin influenza A(H7N9) among 1129 individuals of the general population, whereas >6% of 396 poultry workers were positive (on the basis of a hemagglutination inhibition titer of ? 80) for this subtype, confirming that infected poultry is the principal source of human infections and that subclinical infections are possible. Fourteen days after symptom onset, elevated levels of antibodies to A(H7N9) were found in 65.8% of patients (25/38) who survived but in only 28.6% of those (2/7) who died, suggesting that the presence of antibodies may improve clinical outcome in infected patients.
Related JoVE Video
Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome.
Lancet
PUBLISHED: 04-25-2013
Show Abstract
Hide Abstract
Human infection with avian influenza A H7N9 virus emerged in eastern China in February, 2013, and has been associated with exposure to poultry. We report the clinical and microbiological features of patients infected with influenza A H7N9 virus and compare genomic features of the human virus with those of the virus in market poultry in Zhejiang, China.
Related JoVE Video
Discordant risk: overweight and cardiometabolic risk in Chinese adults.
Obesity (Silver Spring)
PUBLISHED: 03-19-2013
Show Abstract
Hide Abstract
Recent US work identified "metabolically healthy overweight" and "metabolically at risk normal weight" individuals. Less is known for modernizing countries with recent increased obesity.
Related JoVE Video
Sodium intake from various time frames and incident hypertension among Chinese adults.
Epidemiology
PUBLISHED: 03-08-2013
Show Abstract
Hide Abstract
Although it is clear that there are short-term effects of sodium intake on blood pressure, little is known about the most relevant timing of sodium exposure for the onset of hypertension. This question can be addressed only in cohorts with repeated measures of sodium intake.
Related JoVE Video
Removal of fluorine from contaminated soil by electrokinetic treatment driven by solar energy.
Environ Sci Pollut Res Int
PUBLISHED: 02-25-2013
Show Abstract
Hide Abstract
Instead of direct current power supply, a series of electrokinetic remediation experiments driven by solar energy on fluorine-contaminated soil were conducted in a self-made electrolyzer, in order to reduce energy expenditure of electrokinetic remediation. After the 12-day electrokinetic remediation driven by solar energy, the removal efficiency of fluorine was 22.3%, and electrokinetic treatment had an impact on changes in partitioning of fluorine in soil. It proved that the combination of electrokinetics and solar energy was feasible and effective to some extent for the remediation of fluorine-contaminated soil. Meanwhile, the experimental results also indicated that the electromigration was a more dominant transport mechanism for the removal of fluorine from contaminated soil than electroosmosis, and the weather condition was the important factor in affecting the removal efficiency.
Related JoVE Video
Viral agents associated with acute diarrhea among outpatient children in southeastern China.
Pediatr. Infect. Dis. J.
PUBLISHED: 02-15-2013
Show Abstract
Hide Abstract
Acute diarrhea is a leading cause of childhood morbidity and mortality worldwide, but there have been few reports on the causative viruses associated with acute diarrhea among outpatient children in developing countries. This study was conducted to identify the viral agents in outpatient children with acute diarrhea in southeastern China.
Related JoVE Video
Plesiomonas shigelloides Infection in Southeast China.
PLoS ONE
PUBLISHED: 01-01-2013
Show Abstract
Hide Abstract
Plesiomonas shigelloides can cause gastroenteritis and extra-intestinal diseases in humans. However, the prevalence of P. shigelloides infections has not been investigated in China.
Related JoVE Video
Dendritic cells expressing BTLA induces CD8+ T cell tolerance and attenuates the severity of diabetes.
Vaccine
PUBLISHED: 06-22-2011
Show Abstract
Hide Abstract
Numerous evidence demonstrate Type 1 diabetes (T1D) is due to a loss of immune tolerance to islet antigens, and CD8(+) T cells play an important role in the development of T1D in NOD mice. The novel coinhibitory receptor BTLA may have a regulatory role in maintaining peripheral tolerance, however, its role in autoimmune diabetes is unknown. To explore whether the generation of tolerance aiming at BTLA will help therapeutic intervention in T1D, the NOD mice were treated with genetically modified dendritic cells (DCs) expressing BTLA. The results demonstrated that transfer of modified DCs significantly induced CD8(+) T cell tolerance and attenuated the severity of diabetes. The findings suggest that genetically modified DC therapies enhancing the BTLA negative cosignal may prove valuable in treating T1D and other autoimmune diseases.
Related JoVE Video
Consumption of monosodium glutamate in relation to incidence of overweight in Chinese adults: China Health and Nutrition Survey (CHNS).
Am. J. Clin. Nutr.
PUBLISHED: 04-06-2011
Show Abstract
Hide Abstract
It has been hypothesized that monosodium glutamate (MSG), a flavor enhancer, is positively associated with weight gain, which influences energy balance through the disruption of the hypothalamic signaling cascade of leptin action.
Related JoVE Video
Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR.
J. Clin. Microbiol.
PUBLISHED: 01-26-2011
Show Abstract
Hide Abstract
A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.
Related JoVE Video
Related JoVE Video
Down-regulation of zinc transporter 8 in the pancreas of db/db mice is rescued by Exendin-4 administration.
Mol Med Rep
PUBLISHED: 09-01-2010
Show Abstract
Hide Abstract
Recent human genetic studies have revealed that common variants of the zinc transporter 8 (ZnT-8) gene are strongly associated with type 2 diabetes mellitus (T2DM). ZnT-8 has been suggested as a potential candidate in the regulation of insulin secretion in pancreatic ?-cells. In this study, we aimed to explore the expression of ZnT-8 in the development of T2DM. The expression of ZnT-8 was investigated in the pancreas and adipose tissue of homozygous db/db mice compared to heterozygous sibling db/+ mice (n=6-8). Furthermore, the effect of Exendin-4 (an analogue of glucagon-like peptide-1) on ZnT-8 expression was examined in the db/db mice. Exendin-4 or vehicle (control) was administered (i.p., 1 nmol/kg) to diabetic 8-week-old db/db mice daily for 14 days (n=8). The results revealed that ZnT-8 protein levels in the pancreas of db/db mice were reduced, accompanied by a decrease in ZnT-8 mRNA. ZnT-8 mRNA and protein levels were also significantly reduced in the epididymal and visceral fat of the db/db mice. Treatment with Exendin-4 up-regulated ZnT-8 gene expression in the pancreas of the db/db mice, but did not affect its expression in adipose tissue. These findings suggest that ZnT-8 synthesis in the pancreas and adipose tissue is down-regulated in db/db mice. Reduced ZnT-8 production in the pancreas may advance defects in insulin secretion in diabetes. These may be reversed, at least partially, by the administration of Exendin-4.
Related JoVE Video
Prospective randomized controlled study of a Chinese herbal medicine compound Tangzu Yuyang Ointment for chronic diabetic foot ulcers: a preliminary report.
J Ethnopharmacol
PUBLISHED: 06-26-2010
Show Abstract
Hide Abstract
The purpose of this study was to evaluate the efficacy and safety of a topical Chinese herbal medicine (CHM) compound Tangzu Yuyang Ointment (TYO) for treatment of chronic diabetic foot ulcers.
Related JoVE Video
Prospective study on nutrition transition in China.
Nutr. Rev.
PUBLISHED: 05-21-2009
Show Abstract
Hide Abstract
The aim of the prospective study reported here was to examine the effects of social and economic transformation on dietary patterns and nutritional status in China. The study began in 1989 and continued with follow-ups in 1991, 1993, 1997, 2000, and 2004. A total of 5000 subjects aged 18-45 years from 4280 households in nine provinces were included. Weighed records and three consecutive 24-h recalls were used. Over the study period, average consumption of all animal foods except milk increased, while cereal intake decreased. The proportion of animal protein and fat as a percentage of energy also increased. However, vitamin A and calcium intake did not increase and remained low. Child height and weight increased while undernutrition decreased and overweight increased. The results indicate that rapid changes in dietary pattern are associated with economic reforms in China.
Related JoVE Video
Television use and snacking behaviors among children and adolescents in China.
J Adolesc Health
PUBLISHED: 01-03-2009
Show Abstract
Hide Abstract
Television (TV) use has been linked with poor eating behaviors and obesity in young people. This study examines the association between TV watching and paying attention to TV commercials with buying and requesting snacks seen on commercials, and eating snacks while watching TV among youth in China.
Related JoVE Video
Identification of novel HLA-A 0201-restricted cytotoxic T lymphocyte epitopes from Zinc Transporter 8.
Vaccine
Show Abstract
Hide Abstract
Numerous evidences demonstrated that type 1 diabetes (T1D) is due to a loss of immune tolerance to islet antigens, and CD8(+) T cells play an important role in the development of T1D. Zinc Transporter 8 (ZnT8) has emerged in recent years as a target of disease-associated autoreactive T cells in human T1D. However, ZnT8-associated CTL specific-peptides have not been identified. In this study, we predicted and identified HLA-A*0201-restricted cytotoxic T lymphocyte (CTL) epitopes derived from ZnT8, and utilized it to immunize HLA-A2.1/Kb transgenic (Tg) mice. The results demonstrated that peptides of ZnT8 containing residues 107-115, 115-123 and 145-153 could elicit specific CTLs in vitro, and induce diabetes in mice. The results suggest that these specific peptides are novel HLA-A*0201-restricted CTL epitopes, and could have therapeutic potential in preventing of T1D disease.
Related JoVE Video
Residual toxicity and sublethal effects of chlorantraniliprole on Plutella xylostella (lepidoptera: plutellidae).
Pest Manag. Sci.
Show Abstract
Hide Abstract
The diamondback moth (DBM), Plutella xylostella (L.), is the most important pest of cruciferous vegetables in the world. Chlorantraniliprole is a novel anthranilic diamide insecticide registered for the control of lepidopteran pests. The dose response, residual toxicity and sublethal effects of chlorantraniliprole applied for 48 h at LC?? (0.02 mg L(-1) ) and LC?? (0.06 mg L(-1) ) on P. xylostella were investigated.
Related JoVE Video
Peroxisome proliferator-activated receptor-? coactivator-1? (PGC-1?) enhances engraftment and angiogenesis of mesenchymal stem cells in diabetic hindlimb ischemia.
Diabetes
Show Abstract
Hide Abstract
To examine whether the peroxisome proliferator-activated receptor-? coactivator-1? (PGC-1?), a key regulator linking angiogenesis and metabolism, could enhance the engraftment and angiogenesis of mesenchymal stem cells (MSCs) in diabetic hindlimb ischemia, we engineered the overexpression of PGC-1? within MSCs using an adenoviral vector encoding green fluorescent protein and PGC-1?, and then tested the survivability and angiogenesis of MSCs in vitro and in vivo. Under the condition of hypoxia concomitant with serum deprivation, the overexpression of PGC-1? in MSCs resulted in a higher expression level of hypoxia-inducible factor-1? (Hif-1?), a greater ratio of B-cell lymphoma leukemia-2 (Bcl-2)/Bcl-2-associated X protein (Bax), and a lower level of caspase 3 compared with the controls, followed by an increased survival rate and an elevated expression level of several proangiogenic factors. In vivo, the MSCs modified with PGC-1? could significantly increase the blood perfusion and capillary density of ischemic hindlimb of the diabetic rats, which was correlated to an improved survivability of MSCs and an increased level of several proangiogenic factors secreted by MSCs. We identified for the first time that PGC-1? could enhance the engraftment and angiogenesis of MSCs in diabetic hindlimb ischemia.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.