This study was purposed to investigate the expression of miR-16 in T lymphoblastic lymphoma/acute lymphoblastic leukemia (T-LBL/ALL) and its relation with target therapy and prognosis. The CD3, cCD3, CD10, CD20, CD34, CD43, CD99, TdT, PAX-5, BCL-2 and Ki67 in paraffin samples from 38 cases of T-LBL/ALL were detected by immunohistochemical labeling; the miR-16 expression level was detected by real-time RT-PCR. Fifteen cases of reactive hyperplasia of lymph nodes were selected as control. The results indicated that among 38 cases of T-LBL/ALL the positive rate of TdT was highest (94.7%), the positive rate of CD34 was lowest (22.1%), the PAX-5 and CD20 were found to be negative. The Ki67 expression level in 39.5% cases exceeded 80%. As compared with reactive hyperplasia of lymph node, the miR-16 expression in T-LBL/ALL was up-regulated, ant its expression level was 4.87-fold of reactive hyperplasia of lymph node (P < 0.05). The overall survival rate in group of miR-16 high expression decreased (P < 0.05). The prognosis of T-LBL/ALL patients with BCL-2 positive expression was better than that of patients with BCL-2 negative expression (P < 0.05). The miR-16 expression correlated with BCL-2 protein (r = 0.51, P < 0.05). It is concluded that the overall survival rate in miR-16 high expression group is higher than that in miR-16 low expression group, suggesting possible relation of miR-16 with prognosis. Moreover, the prognosis in BCL-2 positive expression group is better than that in negative expression group, which may be a factor influencing prognosis.
This study was aimed to investigate the expression of blood Th17 and CD4(+) CD25(+) regulatory T cells (Treg) in the patients with aplastic anemia(AA). Forty-five patients with AA were enrolled into this study, and were divided into mild aplastic anemia(MAA) group (n = 25) and severe aplastic anemia group(SAA) (n = 20), blood cell count was recorded. 15 healthy donors were enrolled as control. Proportions of blood Th17 and CD4(+) CD25(+) Treg cells were determined by flow cytometry. The serum levels of IL-17, IFN-? and TNF-?, as well as their concentrations in culture supernatant of phytohemagglutinin(PHA) -stimulated peripheral blood mononuclear cells, were measured by ELISA. The results showed that the proportions of blood Th17 cells and concentration of blood serum IL-17 and IFN-? increased in patients with SAA, compared with MAA and normal controls, but CD4(+) CD25(+) Foxp3(+) Treg cells obviously decreased in patients with SAA. The concentrations of IL-17 and IFN-? significantly increased in culture supernatant of SAA group. Hemoglobin level in the patients with AA negatively correlated with the population of Th17 cells and serum IL-17 level, whereas positively correlated with the expression of CD4(+) CD25(+)Treg cells. It is concluded that the increased response of Th17 cells and deficiency of CD4(+) CD25(+) Treg cells present in severe aplastic anemia. The severity of anemia may be related with the imbalance between Th17 and CD4(+) CD25(+)Treg cell response.
Impaired renal function in atherosclerotic renovascular disease (ARD) may be the result of crosstalk between atherosclerotic renovascular stenosis and amplified oxidative stress, inflammation and fibrosis. Berberine (BBR) regulates cholesterol metabolism and exerts antioxidant effects. Accordingly, we hypothesized that BBR treatment may ameliorate ARD-induced kidney injury through its cholesterol-lowering effect and also suppression of the pathways involved in oxidative stress, inflammation and NF?B activation.
An in situ field experiment was conducted to study the effects of simulated nitrogen (N) deposition on soil acid phosphomonoesterase activity (APA) and soil available phosphorous (AP) content in Pinus massoniana forest (PF), coniferous and broad-leaved mixed forest (MF), and monsoon evergreen broad-leaved forest (MEBF) in Dinghushan Mountain. In PF and MF, three treatments were installed, i.e., CK (0 kg N x hm(-2) x a(-1)), low N (50 kg N x hm(-2) x a(-1)), and medium N (100 kg N x hm(-2) x a(-1)); in MEBF, four treatments were installed, i.e., CK, low N, medium N, and high N (150 kg N x hm(-2) x a(-1)). The soil APA and soil AP content decreased with soil depth. The soil APA was the highest in MEBF, while the AP content had no significant difference in the three forests. The effects of N addition on soil APA differed with forest types. In MEBF, the APA was the highest (19.52 micromol x g(-1) x h(-1)) in low N treatment; while in PF and MF, the APA was the highest (12.74 and 11.02 micromol x g(-1) x h(-1), respectively) in medium N treatment. In the three forests, soil AP content was the highest in low N treatment, but had no significant differences among the N treatments. There was a significant positive correlation between soil APA and soil AP content.
This study was designed to evaluate the association between skin autofluorescence (AF), an indicator of advanced glycation end-products (AGEs), and foot ulcers in subjects with diabetes.
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