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Find video protocols related to scientific articles indexed in Pubmed.
Survey on the use of radiotherapy to treat early breast cancer following breast-conserving surgery in China.
Tumori
PUBLISHED: 10-25-2014
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Aims. The use of radiotherapy to treat early stage breast cancer following breast-conserving surgery has markedly increased. The study aimed to evaluate the clinical practice of radiotherapy in China. Materials and methods. A survey concerning the characteristics of breast-conserving radiotherapy for early stage breast cancer was distributed to all radiotherapy departments in mainland China in 2009. The results were analyzed. Results. Three hundred and ninety-six departments replied (41.6%), and 328 (34.4%) launched breast-conserving radiotherapy. Adjuvant chemotherapy followed by radiotherapy was the most common combination and was performed in 55.2% of the responding centers. The median time from surgery to radiotherapy was 9 weeks. Three hundred and nineteen (97.3%) centers treated the whole breast, 273 (83.2%) the supraclavicular area, 138 (43.3%) the axilla, and 85 (26.8%) the internal mammary region; 97.5% (310/319) of all centers performed irradiation of the whole breast in all candidates. One hundred and fourteen (41.8%) treated the supraclavicular area, and 37 (26.8%) treated the axilla in 1-3 positive lymph nodes. Eighty-six (31.5%) and 40 (29.0%) performed the corresponding irradiation in N 2-3 patients. Fifty-six (72.9%) treated the internal mammary region for tumors of the center or inner quadrant. The conformal technique was used in 51.8% of the centers. Conclusions. Although a consensus has been reached, debate still exists about the target of postoperative radiotherapy in early stage breast cancer.
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Pemetrexed is Mildly Active with Good Tolerability for Treatment of Patients with Colorectal Cancer.
Asian Pac. J. Cancer Prev.
PUBLISHED: 10-24-2014
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This systematic analysis was conducted to evaluate the efficacy and safety of pemetrexed based salvage chemotherapy for treatment of patients with metastatic colorectal cancer.
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Chip-off-the-old-rock: the study of reservoir-relevant geological processes with real-rock micromodels.
Lab Chip
PUBLISHED: 09-20-2014
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We present a real-rock micromodel approach whereby microfluidic channels are fabricated in a naturally occurring mineral substrate. The method is applied to quantify calcite dissolution which is relevant to oil/gas recovery, CO2 sequestration, and wastewater disposal in carbonate formations - ubiquitous worldwide. The key advantage of this method is the inclusion of both the relevant substrate chemistry (not possible with conventional microfluidics) and real-time pore-scale resolution (not possible with core samples). Here, microchannels are etched into a natural calcite crystal and sealed with a glass slide. The approach is applied to study acidified brine flow through a single channel and a two-dimensional micromodel. The single-channel case conforms roughly to a 1-D analytical description, with crystal orientation influencing the local dissolution rate an additional 25%. The two-dimensional experiments show highly flow-directed dissolution and associated positive feedback wherein acid preferentially invades high conductivity flow paths, resulting in higher dissolution rates ('wormholing'). These experiments demonstrate and validate the approach of microfabricating fluid structures within natural minerals for transport and geochemical studies. More broadly, real-rock microfluidics open the door to a vast array of lab-on-a-chip opportunities in geology, reservoir engineering, and earth sciences.
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Aspirin Enhances Protective Effect of Fish Oil against Thrombosis and Injury-induced Vascular Remodeling.
Br. J. Pharmacol.
PUBLISHED: 09-15-2014
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Although aspirin (acetylsalicylic acid, ASA) is commonly used to prevent ischemic events in patients with coronary artery disease (CAD), including those undergoing percutaneous coronary intervention (PCI), many patients fail to respond to ASA treatment. Dietary fish oil (FO),containing ?3 polyunsaturated fatty acids (PUFAs), has anti-inflammatory and cardio-protective properties, such as lowering cholesterol and modulating platelet activity. The objective of the present study is to investigate the potential additional effects of ASA and FO on platelet activity and vascular response to injury.
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MiR-492 contributes to cell proliferation and cell cycle of human breast cancer cells by suppressing SOX7 expression.
Tumour Biol.
PUBLISHED: 09-14-2014
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MicroRNAs (miRNAs) have emerged as important regulators that potentially play critical roles in cancer cell biological processes. Previous studies have shown that miR-492 plays an important role in cell tumorigenesis in multiple kinds of human cancer cells. However, the underlying mechanisms of this microRNA in breast cancer remain largely unknown. In the present study, we investigated miR-492's role in cell proliferation of breast cancer. MiR-492 expression was markedly upregulated in breast cancer tissues and breast cancer cells. Overexpression of miR-492 promoted the proliferation and anchorage-independent growth of breast cancer cells. Bioinformatics analysis further revealed sex-determining region Y-box 7 (SOX7), a putative tumor suppressor, as a potential target of miR-492. Data from luciferase reporter assays showed that miR-492 directly binds to the 3'-untranslated region (3'-UTR) of SOX7 messenger RNA (mRNA) and repressed expression at both transcriptional and translational levels. Ectopic expression of miR-492 led to downregulation of SOX7 protein, which resulted in the upregulation of cyclin D1 and c-Myc. In functional assays, SOX7 silenced in miR-492-in-transfected ZR-75-30 cells has positive effect to promote cell proliferation, suggesting that direct SOX7 downregulation is required for miR-492-induced cell proliferation and cell cycle of breast cancer. In sum, these results suggest that miR-492 represents a potential onco-miR and participates in breast cancer carcinogenesis by suppressing SOX7 expression.
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BMP-7 improved proliferation and hematopoietic reconstitution potential of ex vivo expanded cord blood-derived CD34(+) cells.
Hum. Cell
PUBLISHED: 09-06-2014
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Due to limited availability, ex vivo expansion is essential for clinical applications of hematopoietic stem cells (HSCs). Bone morphogenetic proteins (BMPs) play an important role in regulating hematopoiesis development. In this study, the effects of BMP-2 and BMP-7 at different doses on expansion, clonogenicity and differentiation of cord blood (CB)-derived CD34(+) cells were investigated in serum-free medium supplemented with stem cell factor, thrombopoietin and flt3-ligand (STF). Irradiated non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice were used as an animal model to assess the in vivo hematopoietic reconstitution potential of CB-derived CD34(+) cells treated by BMPs. It was demonstrated that the addition of BMP-7 at 5 ng/mL improved the proliferations of total cells, CD34(+) cells and CD34(+)CD38(-) cells without affecting the colony-forming ability of CD34(+) cells and component of lineage cells, while BMP-2 showed no effect on expanding these cells during the 10-day culture. Moreover, CB-derived CD34(+) cells cultured with STF and 5 ng/mL BMP-7 for 10 days were transplanted into irradiated NOD/SCID mice, and showed better engraftment and multi-lineage reconstitution ability compared with the cells cultured with STF alone. Together, 5 ng/mL BMP-7 was beneficial to ex vivo expansion of CB-derived CD34(+) cells for clinical purposes. The results may help improve the existing culture systems and achieve wider application of HSCs.
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[Effects of bisphenol-a on blastocyst development and implantation].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 09-02-2014
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To determine the effects of bisphenol-A (BPA) on blastocyst development and implantation.
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Macrophage-mediated nanoparticle delivery to the periodontal lesions in established murine model via Pg-LPS induction.
J. Oral Pathol. Med.
PUBLISHED: 08-21-2014
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We established a murine periodontitis model by local injection of lipopolysaccharide of Porphyromonas gingivalis (Pg-LPS) into the gingival sulcus of mandibular left incisor four times with 48-h interval. The histological examination of the periodontal tissues demonstrated that significant loss of periodontal bone and ligaments was observed in the lesion side with abundant inflammatory cell infiltration. Two days after the last injection, Cy5-labelled siRNA/chitosan particles were injected intraperitoneally (ip). The chitosan/siRNA particles were taken up by peritoneal macrophages, which subsequently migrated to the inflamed gingival area evaluated by in vivo imaging. The localization of macrophages in the inflamed region was further confirmed by immunofluorescent staining. The present report demonstrates that intragingival injection of Pg-LPS can be used to create an experimental model of periodontal inflammation in mice and that recruitment of macrophages with chitosan/siRNA nanoparticles to the inflamed area opens the possibility of an RNAi-based therapeutic approach using chitosan as a carrier in periodontitis.
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Improved implant osseointegration of a nanostructured titanium surface via mediation of macrophage polarization.
Biomaterials
PUBLISHED: 08-01-2014
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The use of endosseous implanted materials is often limited by undesirable effects that may be due to macrophage-related inflammation. The purpose of this study was to fabricate a nanostructured surface on a titanium implant to regulate the macrophage inflammatory response and improve the performance of the implant. Anodization at 5 and 20 V as well as UV irradiation were used to generate hydrophilic, nanostructured TiO2 surfaces (denoted as NT5 and NT20, respectively). Their surface characteristics and in vivo osseointegration as well as the inflammatory response they elicit were analyzed. In addition, the behavior of macrophages in vitro was evaluated. Although the in vitro osteogenic activity on the two surfaces was similar, the NT5 surface was associated with more bone formation, less inflammation, and a reduced CD68(+) macrophage distribution in vivo compared to the NT20 and polished Ti surfaces. Consistently, further experiments revealed that the NT5 surface induced healing-associated M2 polarization in vitro and in vivo. By contrast, the NT20 surface promoted the pro-inflammatory M1 polarization, which could further impair bone regeneration. The results demonstrate the dominant role of macrophage-related inflammation in bone healing around implants and that surface nanotopography can be designed to have an immune-regulating effect in support of the success of implants.
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[Risk factors of level Ib lymphadenopathy in nasopharyngeal carcinoma].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 07-25-2014
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To analyze the risk factors for level Ib lymph node enlargement on CT in patients with nasopharyngeal carcinoma (NPC) and provide clinical evidence for defining the indications of prophylactic level Ib irradiation.
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Comparison of Hero 642 and K3 rotary nickel-titanium files in curved canals of molars and a systematic review of the literature.
Exp Ther Med
PUBLISHED: 07-18-2014
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The aim of the present study was to compare the root canal preparation ability of rotary nickel-titanium (NiTi) Hero 642 and K3 files in curved mandibular or maxillary molars. A total of 40 extracted mandibular molars with two separate mesial canals, an apical width of approximately size ?15 and a root canal curvature of 15-30° were randomly divided into two groups and instrumented using Hero 642 (n=20) or K3 files (n=20). Canal straightening, working length, transportation, cross-sectional area, minimum dentin thickness and the canal angle curvature degree were examined, and a systematic review of the literature was conducted. No statistically significant differences were observed between the two groups with regard to the mean degree of straightening, mean change in working length, mean transportation, amount of dentin removed or remaining minimum dentin thickness (P>0.05). The canal angle curvature decreased in the two groups postoperatively. The systematic review identified six studies, and overall the two files performed similarly in the majority of categories examined. Therefore, the rotary NiTi Hero 642 and K3 files demonstrated comparable shaping abilities and maintenance of working length.
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Normal mesenteric lymph ameliorates acute kidney injury following lipopolysaccharide challenge in mice.
Ren Fail
PUBLISHED: 07-14-2014
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The kidney is one of the prior damaged organs subjected to severe infection and sepsis shock. Our previous studies have shown that the normal mesenteric lymph (NML) obtained from healthy dogs could alleviate multiple organ injuries following endotoxic shock. In the current study, we further investigated the beneficial effect of NML from healthy mice on acute kidney injury (AKI) induced by lipopolysaccharide (LPS) in mice.
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Synthesis, interaction with DNA and antiproliferative activities of two novel Cu(II) complexes with norcantharidin and benzimidazole derivatives.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 06-23-2014
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Two novel complexes [Cu(L)2(Ac)2]·3H2O (1) (L=N-2-methyl benzimidazole demethylcantharate imide, C16H15N3O3, Ac=acetate, C2H3O2) and [Cu(bimz)2(DCA)] (2) (bimz=benzimidazole, C7H6N2; DCA=demethylcantharate, C8H8O5) were synthesized and characterized by elemental analysis, infrared spectra and X-ray diffraction techniques. Cu(II) ion was four-coordinated in complex 1, Cu(II) ion was five-coordinated in complex 2. A large amount of intermolecular hydrogen-bonding and ?-? stacking interactions were observed in these complex structures. The DNA-binding properties of these complexes were investigated using electronic absorption spectra, fluorescence spectra, viscosity measurements and agarose gel electrophoresis. The interactions between the complexes and bovine serum albumin (BSA) were investigated by fluorescence spectra. The antiproliferative activities of the complexes against human hepatoma cells (SMMC7721) were tested in vitro. And the results showed that these complexes could bind to DNA in moderate intensity via partial intercalation, and complexes 1 and 2 could cleave plasmid DNA through hydroxyl radical mechanism. Title complexes could effectively quench the fluorescence of BSA through static quenching. Meanwhile, title complexes had stronger antiproliferative effect compared to L and Na2(DCA) within the tested concentration range. And complex 1 possessed more antiproliferative active than complex 2.
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Activated protease receptor-2 induces GATA6 expression to promote survival in irradiated colon cancer cells.
Arch. Biochem. Biophys.
PUBLISHED: 05-09-2014
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The resistance to irradiation is common and a great drawback in the treatment of cancer with radiotherapy; the underlying mechanism is unclear. GATA binding protein 6 (GATA6) is associated with the pathogenesis of cancer. This study aims to investigate the role of GATA6 on compromising irradiation effect on HT55 and HT29 cells, 2 colorectal cancer cell lines.
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Thermal preparation of lysozyme-imprinted microspheres by using ionic liquid as a stabilizer.
Anal Bioanal Chem
PUBLISHED: 05-08-2014
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Thermal preparation of lysozyme-imprinted microspheres was firstly investigated by using biocompatible ionic liquid (IL) as a thermal stabilizer. The imprinted microspheres made with IL could obtain the good recognition ability to template protein, whereas the imprinted polymer synthesized in the absence of it had a similar adsorption capacity to the non-imprinted one. Furthermore, the preparation conditions of imprinted polymers (MIPs) including the content of IL, temperature of polymerization, and types of functional monomers and crosslinkers were systematically analyzed via circular dichroism spectrum and activity assay. The results illustrated that using hydroxyethyl acrylate as the functional monomer, ethylene glycol dimethacrylate as the crosslinker, 5 % IL as the stabilizer, and 75 °C as the reaction temperature could retain the structure of template protein as much as possible. The obtained MIPs showed excellent recognition ability to the template protein with the separation factor and selectivity factor value of 4.30 and 2.21, respectively. Consequently, it is an effective way to accurately imprint and separate template protein by cooperatively using circular dichroism spectroscopy and activity assay during the preparation of protein MIPs. The method of utilizing IL to stabilizing protein at high temperature would offer a good opportunity for various technologies to improve the development of macromolecules imprinting.
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[Expression and clinical significance of miR-21 in diffuse large B cell lymphoma].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 04-26-2014
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This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma (DLBCL) paraffin-embedded tissues, and evaluate its potential relevance with clinical characteristics. The expression levels of miR-21 in 26 primary DLBCL and 10 normal lymph node tissue specimens were examined by real-time polymerase chain reaction. The expression of PTEN was detected by immunohistochemical staining. The results indicated that the expression of miR-21 was significantly higher in tumor tissues [6.586(1.10,38.22)] than that in normal tissues [0.791 (0.35,2.87)] (P < 0.05). Among 26 patients with DLBCL the expression of PTEN protein was positive in 6 patients (23%), and was negative in 20 patients (77%). In patients with DLBCL, the expression level of miR-21 was negatively correlated with the level of PTEN protein. The high expression of miR-21 was positively correlated with the level of serum LDH. The expression level of miR-21 in patients with Ann Arbor III-IV stage was obviously higher than that of patients with Ann Arbor I-II stage, but did not correlate with the subtype of patients in clinic (P > 0.05). It is concluded that the expression of miR-21 is high in DLBCL and its overexpression may be related with poor prognosis of DLCBL. These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL.
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Comparing Local TV News with National TV News in Cancer Coverage: An Exploratory Content Analysis.
J Health Commun
PUBLISHED: 04-23-2014
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The authors compared local TV news with national TV news in terms of cancer coverage using a nationally representative sample of local nightly TV and national network TV (i.e., ABC, CBS, NBC, and CNN) cancer news stories that aired during 2002 and 2003. Compared with national TV news, local TV cancer stories were (a) much shorter in length, (b) less likely to report on cancer prevention (i.e., preventive behaviors and screening tests), and (c) less likely to reference national organizations (i.e., National Cancer Institute, American Cancer Society, National Institutes of Health, Centers for Disease Control and Prevention, Food and Drug Administration) that have made clear recommendations about ways to prevent cancer. The implications of these findings for health communication research and cancer education were discussed.
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Perfusion culture-induced template-assisted assembling of cell-laden microcarriers is a promising route for fabricating macrotissues.
Biotechnol J
PUBLISHED: 04-14-2014
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Mass transfer limitation in conventional top-down tissue engineering makes it impossible to fabricate large size viable tissue replacements. In the present study, we aimed at performing a systemic investigation of the assembling process in perfusion culture for fabricating centimeter-scale macrotissues from cell-laden microcarriers following a bottom-up modular approach. Cells (human fibroblasts, human mesenchymal stem cells, or HepG2 cells) were seeded onto microcarriers (Cytopore-2 or CultiSpher S) in spinner flasks and cultured for 14 days and subsequently transferred to a perfusion chamber for assembling. It was found that growth of different cells on different microcarriers varied and aggregation of cell-laden microcarriers was favored with CultiSpher S. After perfusion culture for 14 days, while all microtissues could assemble into integral macrotissues, macrotissues of HepG2 cells were structurally most inferior and the assembling of cell-laden CultiSpher S led to a significant shrinkage. By designing perfusion chamber and using agar-based templates, tubular, disc, and alphabetic letter-shaped macrotissues could be easily fabricated, suggesting template-assisted assembling. Importantly, it was revealed that there existed both optimal perfusion culture time (21 days) and packing condition (1/4 compression) for the assembling of microtissues. This study lays a solid foundation for future applications of this microtissue assembling technique in tissue engineering.
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Involvement of N-cadherin/?-catenin interaction in the micro/nanotopography induced indirect mechanotransduction.
Biomaterials
PUBLISHED: 03-30-2014
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Topographical modification at micro- and nanoscale is widely applied to enhance the tissue integration properties of biomaterials, but the underlying molecular mechanism is poorly understood. The biomaterial topography modulates cell functions via mechanotransduction of direct and indirect. We propose that N-cadherin may play a role in the topographically induced indirect mechanotransduction by regulating the ?-catenin signaling. For confirmation, the cell functions, N-cadherin expression and ?-catenin signaling activation of osteoblasts on titanium (Ti) surfaces with micro- or/and nanotopography are systemically compared with naive and N-cadherin down-regulating MC3T3-E1 cells. We find that the N-cadherin expression is reversely related to the intracellular ?-catenin signaling and the N-cadherin/?-catenin signaling is modulated differentially by the micro- and nanotopography. The nanotopography significantly up-regulates the N-cadherin expression leading to lower ?-catenin signaling activity and consequently depressed differentiation, whereas the microtopography down-regulates the N-cadherin expression resulting in enhanced ?-catenin signaling and thus osteoblast differentiation. Artificial down-regulation of the N-cadherin expression can significantly up-regulate the ?-catenin signaling and consequently enhance the osteoblast differentiation on all the Ti surfaces. The study for the first time clarifies the involvement of the N-cadherin/?-catenin interaction in the micro/nanotopography induced indirect mechanotransduction and provides a potentially new approach for biomaterial modification and biofunctionalization by down-regulating the cell N-cadherin expression to achieve improved clinical performance.
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Hematopoietic repopulating ability of CD34(+) progenitor cells ex vivo expanded with different cytokine combinations.
Artif Cells Nanomed Biotechnol
PUBLISHED: 03-27-2014
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Ex-vivo expansion technologies were developed for application of hematopoietic stem cells (HSCs) derived from cord blood (CB). The cytokine combination was essential to expand HSCs ex vivo and maintain the function of expanded HSCs. However the optimal cytokine combination was not determined. In this study, two combinations of cytokines were applied in ex-vivo expansion of HSCs to investigate the effect on the hematopoietic repopulating ability of expanded HSCs. CB CD34(+) cells were expanded with SCF + TPO + FL (STF) or SCF + TPO + FL + IL-3 + IL-6 (STF36) for 7 days and got 30.3 ± 6.4 and 39.8 ± 7.3 folds of total cells, respectively. The cells cultured by both STF and STF36 could engraft and repopulate in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice effectively; however, the STF group achieved higher level of engraftment. These result demonstrated that the cytokine combination of STF36 favored the expansion of cells, while the cytokine combination of STF facilitated the engraftment and multi-lineage repopulating in vivo. These findings may have important implications for the cell therapy.
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Herbal medicine for hospitalized patients with severe depressive episode: A retrospective controlled study.
J Affect Disord
PUBLISHED: 03-24-2014
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Herbal medicine is increasingly used in depressed patients. The purpose of this retrospective controlled study was to evaluate the efficacy and safety of herbal medicine treatment of severe depressive episode. A total of 146 severely depressed subjects were selected from patients who were admitted to the Department of Psychosomatics of Tongde Hospital at Hangzhou, China between 1st September 2009 and 30th November 2013. While all were medicated with psychotropic drugs, 78 received additional individualized herbal medicine. The severity of depressive symptoms was measured using 24-item Hamilton Rating Scale for Depression (HAMD-24) at admission and thereafter once weekly during hospital stay. The proportion of patients achieving clinical response and remission and incidence of adverse events were compared. The two groups had similar average length of hospital stay for approximately 28 days and were not different in the use of psychotropic medications. Survival analysis revealed that patients with herbal medicine had significantly higher chance of achieving clinical response [relative risk (RR)=2.179, P<0.001] and remission (RR=5.866, P<0.001) compared to those without herbal medicine. Patients with herbal medicine experienced remarkably fewer incidences of physical tiredness, headache, palpitation, dry mouth and constipation, but had a significantly higher incidence of digestive discomfort compared to patients without herbal medicine. These results indicate that additional treatment with individualized herbal medicine enhances antidepressant response and reduces certain side effects associated with psychotropic medications. Herbal medicine is an effective and relatively safe therapy for severe depressive episode (Trial Registration: ChiCTR-OCH-13003864).
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Aberrant brain functional connectivity related to insulin resistance in type 2 diabetes: a resting-state fMRI study.
Diabetes Care
PUBLISHED: 03-21-2014
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Type 2 diabetes is characterized by insulin resistance, which is involved in the development of Alzheimer disease. This study aims to investigate the relationship between abnormal resting-state brain functional connectivity and insulin resistance in type 2 diabetes.
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Determination of dew point conditions for CO2 with impurities using microfluidics.
Environ. Sci. Technol.
PUBLISHED: 03-03-2014
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Impurities can greatly modify the phase behavior of carbon dioxide (CO2), with significant implications on the safety and cost of transport in pipelines. In this paper we demonstrate a microfluidic approach to measure the dew point of such mixtures, specifically the point at which water in supercritical CO2 mixtures condenses to a liquid state. The method enables direct visualization of dew formation (? 1-2 ?m diameter droplets) at industrially relevant concentrations, pressures, and temperatures. Dew point measurements for the well-studied case of pure CO2-water agreed well with previous theoretical and experimental data over the range of pressure (up to 13.17 MPa), temperature (up to 50 °C), and water content (down to 0.00229 mol fraction) studied. The microfluidic approach showed a nearly 3-fold reduction in error as compared to previous methods. When applied to a mixture with nitrogen (2.5%) and oxygen (5.8%) impurities--typical of flue gas from natural gas oxy-fuel combustion processes--the measured dew point pressure increased on average 17.55 ± 5.4%, indicating a more stringent minimum pressure for pipeline transport. In addition to increased precision, the microfluidic method offers a direct measurement of dew formation, requires very small volumes (? 10 ?L), and is applicable to ultralow water contents (<0.005 mol fractions), circumventing the limits of previous methods.
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Phase?I?study of postoperative radiotherapy combined with capecitabine for gastric cancer.
World J. Gastroenterol.
PUBLISHED: 02-28-2014
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To determine the maximum tolerated dose (MTD) and dose-limiting toxicity (DLT) of capecitabine combined with postoperative radiotherapy for gastric cancer.
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Protease-activated receptor-2 modulates hepatic stellate cell collagen release and apoptotic status.
Arch. Biochem. Biophys.
PUBLISHED: 01-22-2014
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The pathogenesis of hepatic fibrosis is to be further investigated. Protease-activated receptor-2 (PAR2) plays a role in hepatic fibrosis. This study aims to elucidate the role of activation of PAR2 in the regulation of hepatic stellate cell activities. In this study, the expression of PAR2, Fas and caveolin-1 in human hepatic stellate cell line, HHStec cell (HHStecs) was assessed by real time RT-PCR and Western blot. The levels of collagen were determined by enzyme-linked immunosorbent assay. The PAR2 gene was silenced in HHStecs using RNA interference. Apoptosis of HHStecs was assessed by flow cytometry. The results showed that HHStecs expressed PAR2, which was up regulated by activation with phorbol myristate acetate (PMA). Activation of PAR2 increased the release of collagen from HHStecs. Exposure to PMA induced HHStec apoptosis, which was significantly inhibited by activation of PAR2. The PAR2 activation also suppressed the expression of caveolin-1 and Fas in HHStecs. Over expression of caveolin-1 in HHStecs blocked PAR2-reduced apoptosis. We conclude that HHStecs express PAR2. Activation of PAR2 increases HHStecs to release collagen and reduces the activation-induced HHStec apoptosis, which can be inhibited by the over expression of caveolin-1.
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The complete mitochondrial genome sequence of Parabramis pekinensis strenosoma (Cypriniformes: Cyprinidae).
Mitochondrial DNA
PUBLISHED: 01-21-2014
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Abstract Parabramis pekinensis strenosoma belongs to the family Cyprinidae. In the present study, we obtain the complete mitochondrial genome of P. pekinensis strenosoma by PCR amplification and DNA sequencing. It is a circular double-stranded DNA molecule of 16,623 base pairs in length, consisting of the typical structure of 22 transfer RNA genes, 13 protein-coding genes, 2 ribosomal RNA genes, as well as 2 main non-coding regions (the control region and the origin of the light strand replication). Compared with Parabramis pekinensis, the two different subspecies share 99.58% nucleotide sequence similarity and the biggest nucleotide sequence discrepancy between homologous genes are observed in ND2 for protein-coding genes and in tRNA-Ala for tRNA-coding genes. The complete mitochondrial genome sequence data are of great use for phylogenetic analysis and studies of population genetics and germplasm resources of P. pekinensis strenosoma.
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Chitosan hydrogel as siRNA vector for prolonged gene silencing.
J Nanobiotechnology
PUBLISHED: 01-18-2014
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The periodontitis is one of the most prevalent diseases with alveolar resorption in adult people and is the main cause of the tooth loss. To investigate the possibility for protecting the loss of alveolar bone in periodontal diseases, a RNAi-based therapeutic strategy is applied for silencing RANK signaling using thermosensitive chitosan hydrogel as siRNA reservoir and vector.
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The complete mitochondrial genome of natural Paramisgurnus dabryanus (Cypriniformes: Cobitidae).
Mitochondrial DNA
PUBLISHED: 01-14-2014
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Abstract Paramisgurnus dabryanus, a small-sized freshwater fish species, is one of the most important cultured fish in East Asia from Russia to China. In this study, the complete mitochondrial genome of P. dabryanus is sequenced to be 16,566?bp in length, including 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, a control region and the origin of the light strand replication. The overall base composition of P. dabryanus in descending order is A 29.66 %, T 28.04%, C 25.81% and G 16.49%, with a slight A?+?T bias. The mitogenome sequence data may provide useful information to the population genetics analysis of P. dabryanus and the elucidation of evolutionary mechanisms in Cobitidae.
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Immobilization of chitosan film containing semaphorin 3A onto a microarc oxidized titanium implant surface via silane reaction to improve MG63 osteogenic differentiation.
Int J Nanomedicine
PUBLISHED: 01-01-2014
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Improving osseointegration of extensively used titanium (Ti) implants still remains a main theme in implantology. Recently, grafting biomolecules onto a Ti surface has attracted more attention due to their direct participation in the osseointegration process around the implant. Semaphorin 3A (Sema3A) is a new proven osteoprotection molecule and is considered to be a promising therapeutic agent in bone diseases, but how to immobilize the protein onto a Ti surface to acquire a long-term effect is poorly defined. In our study, we tried to use chitosan to wrap Sema3A (CS/Sema) and connect to the microarc oxidized Ti surface via silane glutaraldehyde coupling. The microarc oxidization could formulate porous topography on a Ti surface, and the covalently bonded coating was homogeneously covered on the ridges between the pores without significant influence on the original topography. A burst release of Sema3A was observed in the first few days in phosphate-buffered saline and could be maintained for >2 weeks. Coating in phosphate-buffered saline containing lysozyme was similar, but the release rate was much more rapid. The coating did not significantly affect cellular adhesion, viability, or cytoskeleton arrangement, but the osteogenic-related gene expression was dramatically increased and calcium deposition was also abundantly detected. In conclusion, covalent bonding of CS/Sema could strongly improve osteogenic differentiation of osteoblasts and might be applied for Ti implant surface biofunctionalization.
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Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.
PLoS ONE
PUBLISHED: 01-01-2014
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Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. Immunoproteomic is a promising method to identify pathogenicity factors of different diseases. In the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella Pneumonia bacteremia. Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified. Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu). In validation of the pathogenicity factor, we used western blotting to show that Klebsiella pneumonia had higher (EF-Tu) expression when they accompanied by leukopenia rather than leukocytosis. Thus, we report 6 pathogenicity factors of leukopenia caused by Klebsiella pneumonia bacteremia, including 5 housekeeping enzymes and EF-Tu. We suggest EF-Tu could be a potential pathogenicity factor for leukopenia caused by Klebsiella pneumonia.
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Ectopic osteogenesis of macroscopic tissue constructs assembled from human mesenchymal stem cell-laden microcarriers through in vitro perfusion culture.
PLoS ONE
PUBLISHED: 01-01-2014
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We had previously demonstrated the feasibility of preparing a centimeter-sized bone tissue construct by following a modular approach. In the present study, the objectives were to evaluate osteogenesis and tissue formation of human amniotic mesenchymal stem cells-laden CultiSpher S microcarriers during in vitro perfusion culture and after subcutaneous implantation. Microtissues were prepared in dynamic culture using spinner flasks in 28 days. In comparison with 1-week perfusion culture, microtissues became more obviously fused, demonstrating significantly higher cellularity, metabolic activity, ALP activity and calcium content while maintaining cell viability after 2-week perfusion. After subcutaneous implantation in nude mice for 6 and 12 weeks, all explants showed tight contexture, suggesting profound tissue remodeling in vivo. In addition, 12-week implantation resulted in slightly better tissue properties. However, in vitro perfusion culture time exerted great influence on the properties of corresponding explants. Degradation of microcarriers was more pronounced in the explants of 2-week perfused macrotissues compared to those of 1-week perfusion and directly implanted microtissues. Moreover, more blood vessel infiltration and bone matrix deposition with homogeneous spatial distribution were found in the explants of 2-week perfused macrotissues. Taken together, in vitro perfusion culture time is critical in engineering bone tissue replacements using such a modular approach, which holds great promise for bone regeneration.
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Dimethyloxaloylglycine improves angiogenic activity of bone marrow stromal cells in the tissue-engineered bone.
Int. J. Biol. Sci.
PUBLISHED: 01-01-2014
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One of the big challenges in tissue engineering for treating large bone defects is to promote the angiogenesis of the tissue-engineered bone. Hypoxia inducible factor-1? (HIF-1?) plays an important role in angiogenesis-osteogenesis coupling during bone regeneration, and can activate a broad array of angiogenic factors. Dimethyloxaloylglycine (DMOG) can activate HIF-1? expression in cells at normal oxygen tension. In this study, we explored the effect of DMOG on the angiogenic activity of bone mesenchymal stem cells (BMSCs) in the tissue-engineered bone. The effect of different concentrations of DMOG on HIF-1a expression in BMSCs was detected with western blotting, and the mRNA expression and secretion of related angiogenic factors in DMOG-treated BMSCs were respectively analyzed using qRT-PCR and enzyme linked immunosorbent assay. The tissue-engineered bone constructed with ?-tricalcium phosphate (?-TCP) and DMOG-treated BMSCs were implanted into the critical-sized calvarial defects to test the effectiveness of DMOG in improving the angiogenic activity of BMSCs in the tissue-engineered bone. The results showed DMOG significantly enhanced the mRNA expression and secretion of related angiogenic factors in BMSCs by activating the expression of HIF-1?. More newly formed blood vessels were observed in the group treated with ?-TCP and DMOG-treated BMSCs than in other groups. And there were also more bone regeneration in the group treated with ?-TCP and DMOG-treated BMSCs. Therefore, we believed DMOG could enhance the angiogenic activity of BMSCs by activating the expression of HIF-1?, thereby improve the angiogenesis of the tissue-engineered bone and its bone healing capacity.
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Synergism from the combination of ulinastatin and curcumin offers greater inhibition against colorectal cancer liver metastases via modulating matrix metalloproteinase-9 and E-cadherin expression.
Onco Targets Ther
PUBLISHED: 01-01-2014
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Liver metastasis is a major cause of mortality in colorectal cancer (CRC). The current study was to investigate the ability of ulinastatin (UTI) and curcumin (CUR) to inhibit CRC liver metastases via modulating matrix metalloproteinase-9 (MMP-9) and E-cadherin expression. Human CRC HCT-116 cells were treated with compounds individually and in combination in order to understand the effect on cell migration and invasion. The HCT-116 cell line was established to stably express luciferase and green fluorescent protein (GFP) by lentiviral transduction (HCT-116-Luc-GFP). We identified an anti-metastasis effect of UTI and CUR on a CRC liver metastasis mouse model. Tumor development and therapeutic responses were dynamically tracked by bioluminescence imaging. Expression of MMP-9 and E-cadherin in metastatic tumors was detected by immunohistochemical assay. Results of wound healing and cell invasion assays suggest that treatment with UTI, CUR, and UTI plus CUR, respectively, significantly inhibit HCT-116 cell migration and invasion. Furthermore, results of CRC hepatic metastasis on a nude mouse model showed that treatment with UTI, CUR alone, and a combination notably inhibited hepatic metastases from CRC and prolonged survival of tumor-bearing mice, especially in the UTI plus CUR group. These results suggest that the combination of UTI and CUR together may offer greater inhibition against metastasis of CRC.
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[Interventional therapy for lung cancer patients with superior vena cava syndrome].
Zhonghua Zhong Liu Za Zhi
PUBLISHED: 12-10-2013
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To investigate the method, therapeutic effect and safety of interventional therapy for lung cancer patients with superior vena cava syndrome (SVCS).
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Endometrial Intraepithelial Neoplasia (EIN) in Endometrial Biopsy Specimens Categorized by the 1994 World Health Organization Classification for Endometrial Hyperplasia.
Asian Pac. J. Cancer Prev.
PUBLISHED: 12-03-2013
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Our study is to determine the presence of endometrial intraepithelial neoplasia (EIN) in endometrial biopsy specimens classified by the 1994 World Health Organization (WHO) criteria for endometrial hyperplasia. Endometrial biopsy specimens that were stained with hematoxylin and eosin (HE) were examined and categorized by the WHO 1994 criteria and for the presence of EIN as defined by the International Endometrial Collaborative Group. ?-catenin expression was examined by immunohistochemistry. A total of 474 cases of HE stained endometrial biopsy tissues were reviewed. There were 379 cases of simple endometrial hyperplasia, 16 with simple atypical endometrial hyperplasia, 48 with complex endometrial hyperplasia, and 31 with complex atypical endometrial hyperplasia. Among the 474 endometrial hyperplasia cases, there were 46 (9.7%) that were classified as EIN. Of these 46 cases, 11(2.9%) were classified as simple endometrial hyperplasia, 1 (6.3%) as simple atypical endometrial hyperplasia, 6 (12.5%) as complex endometrial hyperplasia, and 28 (90.3%) as complex atypical endometrial hyperplasia. EIN was associated with a higher rate of ?-catenin positivity than endometrium classified as benign hyperplasia (72% vs. 22.5%, respectively, P < 0.001), but a lower rate than endometrial adenocarcinoma (72% vs. 96.2%, respectively, P < 0.001). In benign endometrial hyperplasia, high ?-catenin expression was noted in the cell membranes, whereas in EIN and endometrial adenocarcinoma high expression was noted in the cytoplasm. In conclusion, EIN is more accurate than the WHO classification for the diagnosis of precancerous lesions of the endometrium.
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In Situ Controlled Release of rhBMP-2 in Gelatin-Coated 3D Porous Poly(?-caprolactone) Scaffolds for Homogeneous Bone Tissue Formation.
Biomacromolecules
PUBLISHED: 11-27-2013
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In tissue engineering, incorporation of bone morphogenetic protein-2 (BMP-2) into biomaterial scaffolds is an attractive strategy to stimulate bone repair. However, suboptimal release of BMP-2 remains a great concern, which may cause unfavorable bone formation as well as severe inflammation. In this study, genipin-cross-linked gelatin entrapped with recombinant human BMP-2 (rhBMP-2) was exploited to decorate the interior surface of three-dimensional porous poly(?-caprolactone) (PCL) scaffolds. With gelatin-coating, PCL scaffolds demonstrated enhanced water uptake and improved compressive moduli. Intriguingly, a unique release profile of rhBMP-2 composed of a transient burst release followed by a sustained release was achieved in coated scaffolds. These coated scaffolds well supported growth and osteogenesis of human mesenchymal stem cells (hMSCs) in vitro, indicating the retaining of rhBMP-2 bioactivity. When hMSCs-seeded scaffolds were implanted subcutaneously in nude mice for 4 weeks, better bone formation was observed in gelatin/rhBMP-2-coated scaffolds. Specifically, the spatial distribution of newly formed bone was more uniform in gelatin-coated scaffolds than in uncoated scaffolds, which displayed preferential bone formation at the periphery. These results collectively demonstrated that gelatin-coating of porous PCL scaffolds is a promising approach for delivering rhBMP-2 to stimulate improved bone regeneration.
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Crystal structure of a plant leucine rich repeat protein with two island domains.
Sci China Life Sci
PUBLISHED: 10-15-2013
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Leucine rich repeat (LRR) domain, characterized by a repetitive sequence pattern rich in leucine residues, is a universal protein-protein interaction motif present in all life forms. LRR repeats interrupted by sequences of 30-70 residues (termed island domain, ID) have been found in some plant LRR receptor-like kinases (RLKs) and animal Toll-like receptors (TLR7-9). Recent studies provide insight into how a single ID is structurally integrated into an LRR protein. However, structural information on an LRR protein with two IDs is lacking. The receptor-like protein kinase 2 (RPK2) is an LRR-RLK and has important roles in controlling plant growth and development by perception and transduction of hormone signal. Here we present the crystal structure of the extracellular LRR domain of RPK2 (RPK2-LRR) containing two IDs from Arabidopsis. The structure reveals that both of the IDs are helical and located at the central region of the single RPK2-LRR solenoid. One of them binds to the inner surface of the solenoid, whereas the other one mainly interacts with the lateral side. Unexpectedly, a long loop immediately following the N-terminal capping domain of RPK2-LRR is presented toward and sandwiched between the two IDs, further stabilizing their embedding to the LRR solenoid. A potential ligand binding site formed by the two IDs and the solenoid is located at the C-terminal side of RPK2-LRR. The structural information of RPK2-LRR broadens our understanding toward the large family of LRR proteins and provides insight into RPK2-mediated signaling.
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The complete mitochondrial genome of natural Misgurnus bipartitus (Cypriniformes: Cobitidae).
Mitochondrial DNA
PUBLISHED: 10-15-2013
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Abstract Misgurnus bipartitus, a small sized freshwater fish species, is one of the most important cultured fish in East Asia. In this study, the complete mitochondrial genome of M. bipartitus is sequenced to be 16,636?bp in length, including 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, a control region and the origin of the light strand replication. The overall base composition of M. bipartitus in descending order is A 29.79 %, T 27.95%, C 25.90% and G 16.36%, with a slight A?+?T bias. The mitogenome sequence data may provide useful information to the population genetics analysis of M. bipartitus and the elucidation of evolutionary mechanisms in Cobitidae.
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[Effects of drought stress on the root growth and development and physiological characteristics of peanut].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 09-27-2013
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Taking two peanut varieties Huayu 17 and Tangke 8 as test objects, a soil column culture experiment was conducted in a rainproof tank to study the peanut root morphological development and physiological characteristics at late growth stages under moderate drought and well-watered conditions. Tanke 8 had more developed root system and higher yield and drought coefficient, while Huayu 17 had poorer root adaptability to drought stress. For the two varieties, their root length density and root biomass were mainly distributed in 0-40 cm soil layer, whereas their root traits differed in the same soil layer. The total root length, total root surface area, and total root volume of Huayu 17 at each growth stage were smaller under drought stress than under well-balanced water treatment, while these root characteristics of Tangke 8 under drought stress only decreased at flowering-pegging stage. Drought stress increased the root biomass, surface area, and volume of the two varieties in 20-40 cm soil layer, but decreased these root traits in the soil layers below 40 cm. Under drought stress, the root activity of the two varieties in the soil layers below 40 cm at pod filling stage decreased, and the decrement was larger for Huayu 17. The differences in the root system development and physiological characteristics of the two varieties at late growth stages under drought stress suggested that the root system of the two varieties had different water absorption and utilization under drought stress.
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Complete mitochondrial genome of the Amur weatherfish, Misgurnus mohoity (Teleostei: Cypriniformes: Cobitididae).
Mitochondrial DNA
PUBLISHED: 09-20-2013
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Abstract The complete mitochondrial genome of the Amur weatherfish, Misgurnus mohoity is a circular molecule of 16,566?bp in size, containing 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 2 main non-coding regions (the control region and the origin of the light strand replication). Most of the genes are encoded on the heavy strand, except for ND6 and eight tRNAs. The control region is 915?bp in length and located between the tRNA(Pro) and tRNA(Phe) genes, some typical conserved elements (TAS, CSB1-3 and CSB D-F) were found in this region. All these features reflect a typical vertebrate mitochondrial gene arrangement of the M. mohoity.
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Guest-Cage Atomic Interactions in a Clathrate-based Phase-Change Material.
Adv. Mater. Weinheim
PUBLISHED: 08-21-2013
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We report new clathrate-based phase-change materials with cage-like structures incorporating Cs and Ba guest atoms, as a means of altering crystallization and amorphization behaviour by controlling guest-cage interactions via intra-complex guest vibrational effects. Both a high resistance to spontaneous crystallization, and long retention of the amorphous phase were achieved, as well as a low melting energy. This approach provides a route for achieving cage-controlled semiconductor devices.
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An investigation into the relationship between metabolic responses and energy regulation in antibody-producing cell.
J. Microbiol. Biotechnol.
PUBLISHED: 08-17-2013
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Energy-efficient metabolic responses were often noted in high-productive cultures. To better understand these metabolic responses, an investigation into the relationship between metabolic responses and energy regulation was conducted via a comparative analysis among cultures with different energy source supplies. Both glycolysis and glutaminolysis were studied through the kinetic analyses of major extracellular metabolites concerning the fast and slow cell growth stages, respectively, as well as the time-course profiles of intracellular metabolites. In three cultures showing distinct antibody productivities, the amino acid metabolism and energy state were further examined. Both the transition of lactate from production to consumption and steady intracellular pools of pyruvate and lactate were observed to be correlated with efficient energy regulation. In addition, an efficient utilization of amino acids as the replenishment for the TCA cycle was also found in the cultures with upregulated energy metabolism. It was further revealed that the inefficient energy regulation would cause low cell productivity based on the comparative analysis of cell growth and productivity in cultures having distinct energy regulation.
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Niacin, an old drug with a new twist.
J. Lipid Res.
PUBLISHED: 08-15-2013
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Niacin (nicotinic acid) has been used for decades as a lipid-lowering drug. The clinical use of niacin to treat dyslipidemic conditions is limited by its side effects. Niacin, along with fibrates, are the only approved drugs which elevate high density lipoprotein cholesterol (HDLc) along with its effects on low density lipoprotein cholesterol (LDLc) and triglycerides. Whether niacin has a beneficial role in lowering cardiovascular risk on the background of well-controlled LDLc has not been established. In fact, it remains unclear whether niacin, either in the setting of well-controlled LDLc or in combination with other lipid-lowering agents, confers any therapeutic benefit and if so, by which mechanism. The results of recent trials reject the hypothesis that simply raising HDLc is cardioprotective. However, in the case of the clinical trials, structural limitations of trial design complicate their interpretation. This is also true of the most recent Heart Protection Study 2-Treatment of HDLc to Reduce the Incidence of Vascular Events (HPS2-THRIVE) trial in which niacin is combined with an antagonist of the D prostanoid (DP) receptor. Human genetic studies have also questioned the relationship between cardiovascular benefit and HDLc. It remains to be determined whether niacin may have clinical utility in particular subgroups, such as statin intolerant patients with hypercholesterolemia or those who cannot achieve a sufficient reduction in LDLc. It also is unclear whether a potentially beneficial effect of niacin is confounded by DP antagonism in HPS2-THRIVE.
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The complete nucleotide sequence of white Amur bream (Parabramis pekinensis) mitochondrial genome.
Mitochondrial DNA
PUBLISHED: 07-31-2013
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Abstract White Amur bream, Parabramis pekinensis (Cypriniformes: Cyprinidae), a freshwater cyprinid fish, is an important economic fish in several countries, especially in China. The complete sequence of P. pekinensis mitochondrial genome has been determined. The genome is 16,622?bp in length, and consists of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and the noncoding control region, with the genomic organization being identical to that of typical vertebrates. Three conserved sequence blocks (CSB1 to CSB3) were identified in the control region. The complete mitochondrial genome sequence is useful for phylogenetic analysis and studies of population genetics of P. pekinensis.
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MTA1 promotes nasopharyngeal carcinoma growth in vitro and in vivo.
J. Exp. Clin. Cancer Res.
PUBLISHED: 07-26-2013
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The prognostic value of metastasis-associated gene 1 (MTA1) in nasopharyngeal carcinoma (NPC) has been suggested. However, there is still no direct evidence that MTA1 promotes NPC growth in vivo. In this study, we aimed to investigate the function of MTA1 in the regulation of NPC cell proliferation and tumorigenesis in vitro and in vivo.
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High-Dose and Extended-Field Intensity Modulated Radiation Therapy for Early-Stage NK/T-Cell Lymphoma of Waldeyers Ring: Dosimetric Analysis and Clinical Outcome.
Int. J. Radiat. Oncol. Biol. Phys.
PUBLISHED: 07-18-2013
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To assess the dosimetric benefit, treatment outcome, and toxicity of high-dose and extended-field intensity modulated radiation therapy (IMRT) in patients with early-stage NK/T-cell lymphoma of Waldeyers ring (WR-NKTCL).
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Synthesis, interaction with DNA and antiproliferative activities of two novel Cu(II) complexes with Schiff base of benzimidazole.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 07-12-2013
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Two novel copper(II) complexes with Schiff base of benzimidazole [Cu(L)Cl]2·CH3OH have been synthesized. HL(1) (N-(benzimidazol-2-ymethyl)-5-chlorosalicylideneimine, C15H11ClN3O) and HL(2) (N-(benzimidazol-2-ymethyl)-salicylideneimine, C15H12N3O) are ligands of complex (1) and complex (2), respectively. The complexes were characterized by elemental analysis, IR, UV-Vis, TGA and X-ray diffraction. Within the complexes, Cu(II) ions were four coordinated by two nitrogen atom of azomethine and imine, one phenolic oxygen atom from HL and one chloride atom. A distorted quadrilateral structure was formed. Complex (1) crystallized in the triclinic crystal system. Results showed that ?-? stacking effect occurred due to the existence of aromatic ring from Schiff base and hydrogen bonding between methanol and adjacent atoms. The DNA binding properties of the complexes were investigated by electronic absorption spectra, fluorescence spectra and viscosity measurements. Results indicated that complexes bound to DNA via partial intercalation mode. The DNA binding constants Kb/(Lmol(-1)) were 1.81×10(4) (1), 1.37×10(4) (2), 6.27×10(3) (HL(1)) and 3.14×10(3) (HL(2)) at 298K. The title complexes could quench the emission intensities of EB-DNA system significantly. The results of agarose gel electrophoresis indicated complex (1) could cleave supercoiled DNA through the oxidative mechanism. The inhibition ratios revealed that complex (1) and HL(1) had strong antiproliferative activities against human breast cancer cells (MCF-7) lines and human colorectal cancer cells (COLO205) lines in vitro. The antiproliferative activities of complex (1) against MCF-7 lines (IC50=16.9±1.5?molL(-1)) and against COLO205 lines (IC50=16.5±3.4?molL(-1)) is much stronger than that of HL(1), which had the potential to develop anti-cancer drug.
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RUNX3 is a prognostic marker and potential therapeutic target in human breast cancer.
J. Cancer Res. Clin. Oncol.
PUBLISHED: 06-25-2013
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To evaluate the role of RUNX3 in breast cancer pathogenesis, we examined the RUNX3 expression in breast cancer tissues and analyzed the correlation between RUNX3 expression and clinicopathologic variables and patients survival.
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Omega-3 PUFA supplementation and the response to evoked endotoxemia in healthy volunteers.
Mol Nutr Food Res
PUBLISHED: 05-21-2013
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Fish oil-derived n-3 PUFA may improve cardiometabolic health through modulation of innate immunity. However, findings in clinical studies are conflicting. We hypothesized that n-3 PUFA supplementation would dose-dependently reduce the systemic inflammatory response to experimental endotoxemia in healthy humans.
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Ectoparasitic insects and mites on Yunnan red-backed voles (Eothenomys miletus) from a localized area in southwest China.
Parasitol. Res.
PUBLISHED: 04-24-2013
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Ectoparasitic insects and mites on Yunnan red-backed voles (Eothenomys miletus) in Dali prefecture, Yunnan Province, southwest China, were studied between 2003 and 2004. In total, 34,389 individuals of 86 species of ectoparasitic insects (seven species of fleas and five species of sucking lice) and mites (23 species of gamasid mites and 51 species of chigger mites) were collected from 916 individual hosts. The diversity of ectoparasites on this single rodent species in such a small area was much higher than in previous reports, which concerned more host species and greater geographical areas. The majority of the ectoparasites were chigger mites, which accounted for 59.3% of the parasite species and 87.4% of the individual parasites. Most voles harbored parasites with an overall prevalence (P) of 82.5% and mean abundance (MA) of 37.5 parasites per host. The dispersion coefficient (C) and patchiness index (m*/m) were used to study the spatial patterns of the seven dominant parasite species, and all seven had aggregated distributions. The species abundance distribution of the ectoparasites on the vole was fitted by Prestons lognormal distribution (R (2) = 0.82), and the total expected parasite species was estimated from this plot as 167 species. Yunnan red-backed voles harbor many ectoparasites as revealed by examination of a large host population. Future field investigations should sample large numbers of host individuals to assess ectoparasite populations.
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MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity.
ACS Appl Mater Interfaces
PUBLISHED: 03-21-2013
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Developing biomedical titanium (Ti) implants with high osteogenic ability and consequent rigid osseointegration is a constant requirement from the clinic. In this study, we fabricate novel miRNA functionalized microporous Ti implants by lyophilizing miRNA lipoplexes onto a microporous titanium oxide surface formed by microarc oxidation (MAO). The microporous titanium oxide surface provides a larger surface area for miRNA loading and enables spatial retention of the miRNAs within the pores until cellular delivery. The loading of lipoplexes into the micropores on the MAO Ti surface is facilitated by the superhydrophilicity and Ti-OH groups gathering of the MAO surface after UV irradiation followed by lyophilization. A high miRNA transfection efficiency was observed in mesenchymal stem cells (MSCs) seeded onto the miRNA functionalized surface with no apparent cytotoxicity. When functionalizing the Ti surface with miR-29b that enhances osteogenic activity and antimiR-138 that inhibits miR-138 inhibition of endogenous osteogenesis, clear stimulation of MSC osteogenic differentiation was observed, in terms of up-regulating osteogenic expression and enhancing alkaline phosphatase production, collagen secretion and ECM mineralization. The novel miRNA functionalized Ti implants with enhanced osteogenic activity promisingly lead to more rapid and robust osseointegration of a clinical bone implant interface. Our study implies that lyophilization may constitute a versatile method for miRNA loading to other biomaterials with the aim of controlling cellular function.
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Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala.
Fish Physiol. Biochem.
PUBLISHED: 03-18-2013
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This study established and characterized a new cell line (MAF) from the fin of blunt snout bream (Megalobrama amblycephala), a freshwater fish cultivated in China. MAF cells proliferated well in medium 199 supplemented with 10 % fetal bovine serum at 28 °C and have been subcultured more than 95 times in almost a year. MAF cells were revived at 90-95 % viability after 3-6 months of storage in liquid nitrogen. Karyotyping indicated that the modal chromosome number of MAF cells was 48. The MAF cell line consisted predominantly of fibroblastic and epithelial-like cells from M. amblycephala, which was confirmed by immunofluorescence and mitochondrial 12s rRNA sequencing. Viral susceptibility tests showed that MAF cells were susceptible to infection by snakehead rhabdovirus, spring viremia carp virus, and channel catfish virus, which was demonstrated by the presence of cytopathic effect, high viral titers, and PCR products. Bacterial cytotoxicity studies showed that extracellular products from Aeromonas hydrophila were toxic to MAF cells. Cu²? was also cytotoxic to MAF cells, and the 24-h IC?? value was 144.48 ?mol/l. When MAF cells were transfected with pEGFP-N1 plasmid, bright fluorescent signals were observed, and the transfection efficiency reached up to 5 %. These results suggest that the MAF cell line may provide a valuable tool for studying virus pathogenesis, as well as cytotoxicity testing and genetic manipulation studies.
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Tumor suppressor in lung cancer-1 (TSLC1) mediated by dual-regulated oncolytic adenovirus exerts specific antitumor actions in a mouse model.
Acta Pharmacol. Sin.
PUBLISHED: 03-18-2013
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The tumor suppressor in lung cancer-1 (TSLC1) is a candidate tumor suppressor of lung cancer, and frequently inactivated in primary non-small cell lung cancer (NSCLC). In this study, we investigated the effects of TSLC1 mediated by a dual-regulated oncolytic adenovirus on lung cancer, and the mechanisms underlying the antitumor actions.
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[Clinical characteristics and drug resistance in children infected with Pseudomonas aeruginosa in the pediatric intensive care unit].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 02-23-2013
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To study the clinical characteristics of Pseudomonas aeruginosa (PA)-positive children in the pediatric intensive care unit, and to provide a basis for early diagnosis and reasonable treatment of PA infection.
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Interaction with biomacromolecules and antiproliferative activities of Mn(II), Ni(II), Zn(II) complexes of demethylcantharate and 2,2-bipyridine.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 02-17-2013
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Three new transition metal complexes [Mn2(DCA)2(bipy)2]·5H2O (1), [M2(DCA)2(bipy)2(H2O)]·10H2O(M=Ni(II)(2);Zn(II)(3)), (DCA=demethylcantharate, 7-oxabicyclo[2,2,1]heptane-2,3-dicarboxylate, C8H8O5) were synthesized and characterized by elemental analysis, molar conductance, infrared spectra and X-ray diffraction techniques. Each metal ion was six-coordinated in complexes. Complex 1 has a Mn2O2 center. Complexes 2 and 3 have asymmetric binuclear structure. Great amount of intermolecular hydrogen-bonding and ?-?(*) stacking interactions were formed in these complex structures. The DNA-binding properties of complexes were investigated by electronic absorption spectra and viscosity measurements. The DNA binding constants Kb/(Lmol(-1)) were 1.71×10(4) (1), 2.62×10(4) (2) and 1.59×10(4) (3) at 298 K. The complexes could quench the intrinsic fluorescence of bovine serum albumin (BSA) strongly through static quenching. The protein binding constants Ka/(L mol(-1)) were 7.27×10(4) (1), 4.55×10(4) (2) and 7.87×10(4) L mol(-1) (3) and binding site was one. The complexes bind more tightly with DNA and BSA than with ligands. Complexes 1 and 3 had stronger inhibition ratios than Na2(DCA) against human hepatoma cells (SMMC-7721) lines and human gastric cancer cells (MGC80-3) lines in vitro. Complex 3 showed the strongest antiproliferative activity against SMMC-7721 (IC50=29.46±2.12 ?mol L(-1)) and MGC80-3 (IC50=27.02±2.38 ?mol L(-1)), which shows potential in anti-cancer drug development.
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Mesenchymal stem cells downregulate articular chondrocyte differentiation in noncontact coculture systems: implications in cartilage tissue regeneration.
Stem Cells Dev.
PUBLISHED: 02-15-2013
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While chondrogenesis of mesenchymal stem cells (MSCs) in vitro has been extensively studied, their participation in cartilage tissue repair remains unresolved. This study was designed to elucidate if MSCs affect the phenotype of articular chondrocytes (ACs). A combination of noncontact coculture modes was developed. Human or rabbit MSCs and rabbit ACs (rACs) were encapsulated in alginate hydrogel beads [three-dimensional (3D)] or cultured in a monolayer [two-dimensional (2D)] and subsequently cocultured in the Transwell(®) system. After coculture, cell morphology, growth, deposition of the cartilaginous extracellular matrix (ECM), and gene expression of rACs were investigated. It was found that upon coculture without a cell-cell contact, both 2D and 3D cultured MSCs dramatically induced the morphological transformation of 2D cultured rACs from round to a spindle-like shape, and however inhibited the generation of cellular aggregates of 3D cultured rACs. Most strikingly, a coculture resulted in a significantly less deposition of the cartilaginous ECM, including glycosaminoglycans and collagen type II by both 2D and 3D cultured rACs. Importantly, similar observations were achieved for rACs cultured in an MSC-conditioned medium, confirming the definite paracrine interactions between MSCs and rACs. Based on the analysis of gene expression, this phenotypic change of rACs was not identical as the dedifferentiation. To the best of our knowledge, this is a first study demonstrating that MSCs could downregulate chondrocytic differentiation of ACs and warrants considerations in cartilage tissue repair.
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Ulinastatin reduces cancer recurrence after resection of hepatic metastases from colon cancer by inhibiting MMP-9 activation via the antifibrinolytic pathway.
Biomed Res Int
PUBLISHED: 01-28-2013
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High recurrence of colon cancer liver metastasis is observed in patients after hepatic surgery, and the cause is believed to be mostly due to the growth of residual microscopic metastatic lesions within the residual liver. Therefore, triggering the progression of occult metastatic foci may be a novel strategy for improving survival from colon cancer liver metastases. In the present study, we identified an anti-recurrence effect of ulinastatin on colon cancer liver metastasis in mice after hepatectomy. Transwell cell invasion assays demonstrated that ulinastatin significantly inhibited the in vitro invasive ability of colon cancer HCT116 cells. Moreover, gelatin zymography and ELISA analysis showed that MMP-9 activity and plasmin activity of colon cancer HCT116 cells were inhibited by ulinastatin, respectively. Furthermore, in vivo BALB/C nu/nu mice model indicated that ulinastatin effectively reduced recurrence after resection of hepatic metastases from colon cancer. The optimum timing for ulinastatin administration was one week after hepatectomy. Taken together, our findings point to the potential of ulinastatin as an effective approach in controlling recurrence of hepatic metastases from colon cancer after hepatectomy via its anti-plasmin activity.
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Favorable outcome with doxorubicin-based chemotherapy and radiotherapy for adult patients with early stage primary systemic anaplastic large-cell lymphoma.
Eur. J. Haematol.
PUBLISHED: 01-27-2013
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The aim of this study was to analyze outcomes in adult patients with early stage systemic anaplastic large-cell lymphoma (ALCL) treated with doxorubicin-based chemotherapy and radiotherapy. Forty-six adult patients with early stage systemic ALCL received chemotherapy followed by radiotherapy. All patients except two received chemotherapy with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) or a CHOP-like regimen. Twenty patients had stage I disease, and 26 patients had stage II disease. The 5-yr overall survival (OS), progression-free survival (PFS), and local control rates for all patients were 84.4%, 63.6%, and 90.8%, respectively. The 5-yr OS and PFS rates were 95.0% and 77.4% for Ann Arbor stage I disease, and 75.1% and 51.7% for stage II disease, respectively. Lymph node involvement was the main pattern of disease progression or relapse for these patients. Adult patients with early stage systemic ALCL treated with doxorubicin-based chemotherapy and radiotherapy had a favorable prognosis.
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Taxonomy, morphology and molecular systematics of three oligotrich ciliates, including a description of Apostrombidium parakielum spec. nov. (Ciliophora, Oligotrichia).
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 01-04-2013
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Three oligotrich ciliates, Apostrombidium parakielum spec. nov., Novistrombidium apsheronicum (Alekperov & Asadullayeva, 1997) Agatha, 2003 and Novistrombidium testaceum (Anigstein, 1914) Song & Bradbury, 1998 were collected from the coastal waters of China and their morphology and small-subunit rRNA (SSU rRNA) gene sequences were studied. The novel species can be recognized by the combination of its obconical body shape, 14-16 anterior and 6-8 ventral membranelles, somatic kinety in three parts and conspicuously long dorsal cilia. Based on the data obtained for this novel species, an improved diagnosis of the genus Apostrombidium is supplied. Descriptions of the population of N. apsheronicum and N. testaceum collected in this study are also provided and compared with the existing descriptions. In addition, the phylogenetic positions of these three species are inferred from their SSU rRNA gene sequence data. The results indicate that the genus Apostrombidium, the systematics of which has not previously been discussed using molecular information, clusters with Varistrombidium kielum and Omegastrombidium elegans, whereas N. testaceum and N. apsheronicum form a single clade.
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Induction of osteogenic differentiation of stem cells via a lyophilized microRNA reverse transfection formulation on a tissue culture plate.
Int J Nanomedicine
PUBLISHED: 01-01-2013
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MicroRNA (miRNA) regulation is a novel approach to manipulating the fate of mesenchymal stem cells, but an easy, safe, and highly efficient method of transfection is required. In this study, we developed an miRNA reverse transfection formulation by lyophilizing Lipofectamine 2000-miRNA lipoplexes on a tissue culture plate. The lipoplexes can be immobilized on a tissue culture plate with an intact pseudospherical structure and lyophilization without any lyoprotectant. In this study, reverse transfection resulted in highly efficient cellular uptake of miRNA and enabled significant manipulation of the intracellular target miRNA level. Reverse transfection formulations containing Lipofectamine 2000 1 ?L per well generated much higher transfection efficiency without obvious cytotoxicity compared with conventional and other transfection methods. Further, the transfection efficiency of the reverse transfection formulations did not deteriorate during 90 days of storage at 4°C and -20°C. We then assessed the efficiency of the miRNA reverse transfection formulation in promoting osteogenic differentiation of mesenchymal stem cells. We found that transfection with anti-miR-138 and miR-148b was efficient for enhancing osteogenic differentiation, as indicated by enhanced osteogenesis-related gene expression, amount of alkaline phosphatase present, production of collagen, and matrix mineralization. Overall, the miRNA reverse transfection formulation developed in this study is a promising approach for miRNA transfection which can control stem cell fate and is suitable for loading miRNAs onto various biomaterials.
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The extent of cutaneous lesions predicts outcome in extranodal nasal-type natural killer/T-cell lymphoma of the upper aerodigestive tract with secondary cutaneous involvement.
Leuk. Lymphoma
PUBLISHED: 12-13-2011
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This study determined the clinical characteristics and prognosis for patients with extranodal nasal-type natural killer/T-cell lymphoma (NKTCL) with secondary cutaneous involvement. Twenty-eight patients with NKTCL of the upper aerodigestive tract with secondary cutaneous involvement were reviewed. The median overall survival (OS) was 21.5 months from the first diagnosis, and 12.3 months from the presentation of a cutaneous lesion. The 5-year OS rate was 43.1% (median, 28 months) for patients with localized cutaneous disease compared with 0% (median, 3.6 months) for generalized cutaneous disease (p = 0.017). The 2-year OS rates were 67.5% for patients who achieved a complete response (CR) compared with 19.4% (median, 5.2 months) for patients who did not (p = 0.003). Patients with NKTCL with secondary cutaneous dissemination overall have a poor prognosis, but a relatively favorable prognosis was identified for the small subgroup of patients who had localized cutaneous lesions and achieved a CR.
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Diaqua-bis-(5-carb-oxy-2-propyl-1H-imidazole-4-carboxyl-ato-?N,O)cadmium N,N-dimethyl-formamide disolvate.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 11-21-2011
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In the title complex, [Cd(C(8)H(9)N(2)O(4))(2)(H(2)O)(2)]·2C(3)H(7)NO, the six-coordinate Cd(II) ion is in a slightly distorted octa-hedral environment, defined by two O atoms from two coordinated water mol-ecules and two carboxyl-ate O atoms and two N atoms from two N,O-bidentate 5-carb-oxy-2-propyl-1H-imidazole-4-carboxyl-ate ligands. In the crystal, complex mol-ecules and dimethyl-formamide solvent mol-ecules are linked by O-H?O and N-H?O hydrogen bonds into a two-dimensional supra-molecular structure. The propyl groups of the ligands are disordered over two conformations with refined occupancies of 0.680?(7) and 0.320?(7).
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Correlation of antibody production rate with glucose and lactate metabolism in Chinese hamster ovary cells.
Biotechnol. Lett.
PUBLISHED: 08-31-2011
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A linear relationship was found between the antibody production rate (q(mAb)) and the glucose and lactate consumption rate (q(GL)) in Chinese hamster ovary cells. Under a series of q(mAb)-perturbing conditions, q(GL) was determined and a linear relationship between q(mAb) and q(GL) was further established (R(2) = 0.914). Mitochondrial dehydrogenase activity was monitored in all the q(mAb)-perturbing conditions and showed a linear correlation with q(GL) (R(2) = 0.874) as well as with q(mAb). Taken collectively, our results establish that the metabolic parameter, q(GL), is linearly correlated with q (mAb); this finding strengthens our current understanding of process optimization for antibody production.
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catena-Poly[[diaqua-strontium]-bis-(?-quinoline-3-carboxyl-ato)].
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 08-29-2011
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The title compound, [Sr(C(10)H(6)NO(2))(2)(H(2)O)(2)](n), contains an eight-coordinate Sr(II) ion displaying a distorted square-anti-prismatic geometry, two quinoline-3-carboxyl-ate ligands and two terminal water mol-ecules. The Sr(II) atom is surrounded by six carboxyl-ate O atoms from four separate quinoline-3-carboxyl-ate ligands and two O atoms from two coordinated water mol-ecules. The bridging carboxyl-ate O atoms [Sr-O = 2.498?(3) and 2.495?(3)?Å] link Sr(II) atoms, forming a chain substructure extending along the c axis. The chains are linked by O-H?N and O-H?O hydrogen bonds, giving a three-dimensional framework structure.
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Kidney transplant for autosomal dominant polycystic kidney disease: the superiority of concurrent bilateral nephrectomy.
Urol. Int.
PUBLISHED: 06-11-2011
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To assess the transplant outcome of patients who underwent concurrent bilateral nephrectomies (CBN) during kidney transplantation (KT) owing to autosomal dominant polycystic kidney disease (ADPKD).
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[Study on subtype of human papillomavirus infection among aged 25 - 54 reproductive women in Beijing from 2006 to 2008].
Zhonghua Fu Chan Ke Za Zhi
PUBLISHED: 05-18-2011
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To investigate subtype of HPV infection among women at age of 25 to 54 years in Beijing.
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AICAR, a small chemical molecule, primes osteogenic differentiation of adult mesenchymal stem cells.
Int J Artif Organs
PUBLISHED: 05-17-2011
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The chemical approach to controlling stem cell fates is emerging as a powerful tool, holding great promise in tissue engineering and regenerative medicine. Various small molecules have been demonstrated capable of modulating stem cell differentiation. In this paper, we studied the effects of 5-aminoimidazole-4-carboxamide-1-ß-riboside (AICAR), an activator of AMP-activated protein kinase (AMPK), on mesenchymal stem cells (MSCs). AICAR at high concentrations (1.0-2.0 mM) significantly inhibited proliferation of both human amnion-derived MSCs (hAMSCs) and rabbit bone marrow-derived MSCs (BM-MSCs). Most importantly, AICAR efficiently promoted the osteogenic differentiation of hAMSCs and BM-MSCs in both growth medium and osteogenic medium. However, Metformin, another AMPK activator, showed no such effects. Meanwhile, AICAR significantly inhibited adipogenic differentiation of hAMSCs and BM-MSCs. Our data suggests that AICAR represents a potent molecule, which can be applied in bone tissue regeneration.
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Postmastectomy chest wall radiotherapy with single low-energy electron beam: An assessment of outcome and prognostic factors.
Pract Radiat Oncol
PUBLISHED: 05-06-2011
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This study aimed to evaluate the outcome of patients who had received postmastectomy chest wall radiotherapy using a single electron beam, and to identify the relevant factors that influenced prognosis.
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Clinical behavior and treatment outcome of primary nasal diffuse large B-cell lymphoma.
Cancer
PUBLISHED: 05-04-2011
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Nasal diffuse large B-cell lymphoma (DLBCL) is rare. The objective of this study was to evaluate the clinical features and treatment outcomes of patients with nasal DLBCL.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.