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Find video protocols related to scientific articles indexed in Pubmed.
The performance of transrectal ultrasound in the diagnosis of seminal vesicle defects: a comparison with magnetic resonance imaging.
Asian J. Androl.
PUBLISHED: 10-23-2014
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Obstructive azoospermia (OA) is one of the most common causes of male infertility. Transrectal ultrasound (TRUS) has been used to diagnose OA for many years. From 2009 to 2013, we evaluated a prospective cohort of 1249 patients with suspected OA using TRUS. It was found that dilation of the ejaculatory duct (ED) (29.9%, 374/1249) was the most common cause of OA, followed by seminal vesicle (SV) abnormalities (28.5%, 356/1249). A total of 237 patients were diagnosed with congenital defects (agenesis and/or hypoplasia) of the SV, constituting more than half of the cases of SV disease in OA (19.0%, 237/1249). In contrast to ED, congenital defects of the SV could not be corrected with surgical treatment. Therefore, it is meaningful to compare TRUS and magnetic resonance imaging (MRI) for accurate diagnosis of SV defects. Among our patients, 30 with agenesis or/and hypoplasia of the SV on TRUS were further evaluated using pelvic MRI within 2 years, with the objective of verifying the TRUS results. The concordance rate for diagnosing congenital defects of the SV was 73.3% (22/30). We concluded that TRUS is a reliable and convenient method for diagnosing agenesis or hypoplasia of the SV in OA patients with a high concordance with MRI while MRI is useful in patients with inconclusive TRUS findings.
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Effects of a restricted fetal growth environment on human kidney morphology, cell apoptosis and gene expression.
J Renin Angiotensin Aldosterone Syst
PUBLISHED: 10-02-2014
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Kidney development is key to the onset of hypertension and cardiovascular diseases in adults, and in the fetal stage will be impaired by a lack of nutrients in utero in animal models. However, few human studies have been performed.
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Kinetics and thermodynamics of the sorption of furaltadone from aqueous solutions on magnetic multi-walled carbon nanotubes.
Water Sci. Technol.
PUBLISHED: 09-27-2014
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Magnetic multi-wall carbon nanotubes (M-MWCNTs) were used as an adsorbent for removal of furaltadone from aqueous solutions, and the adsorption behaviors were investigated by varying pH, sorbent amount, sorption time and temperature. The results showed that the adsorption efficiency of furaltadone reached 97% when the dosage of M-MWCNT was 0.45 g · L?¹, the pH was 7 and the adsorption time was 150 min. The kinetic data showed that the pseudo-second-order model can fit the adsorption kinetics. The sorption data could be well explained by the Langmuir model under different temperatures. The adsorption process was influenced by both intraparticle diffusion and external mass transfer. The experimental data analysis indicated that the electrostatic attraction and ?-? stacking interactions between M-MWCNT and furaltadone might be the adsorption mechanism. Thermodynamic analysis reflected that adsorption of furaltadone on the M-MWCNT was spontaneous and exothermic. Our study showed that M-MWCNTs can be used as a potential adsorbent for removal of furaltadone from water and wastewater.
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Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules.
Nature
PUBLISHED: 09-23-2014
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The prominent and evolutionarily ancient role of the plant hormone auxin is the regulation of cell expansion. Cell expansion requires ordered arrangement of the cytoskeleton but molecular mechanisms underlying its regulation by signalling molecules including auxin are unknown. Here we show in the model plant Arabidopsis thaliana that in elongating cells exogenous application of auxin or redistribution of endogenous auxin induces very rapid microtubule re-orientation from transverse to longitudinal, coherent with the inhibition of cell expansion. This fast auxin effect requires auxin binding protein 1 (ABP1) and involves a contribution of downstream signalling components such as ROP6 GTPase, ROP-interactive protein RIC1 and the microtubule-severing protein katanin. These components are required for rapid auxin- and ABP1-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses.
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Recurrence of arrhythmia following short-term oral AMIOdarone after CATheter ablation for atrial fibrillation: a double-blind, randomized, placebo-controlled study (AMIO-CAT trial).
Eur. Heart J.
PUBLISHED: 09-02-2014
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Patients undergoing catheter ablation for atrial fibrillation (AF) often experience recurrent arrhythmias within the first few months post-ablation. We aimed to investigate whether short-term use of amiodarone to prevent early arrhythmias following radiofrequency ablation for AF could reduce later recurrence.
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Comparison between laparoscopic partial nephrectomy and laparoscopic ablation therapy: A meta-analysis.
Minim Invasive Ther Allied Technol
PUBLISHED: 09-02-2014
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Abstract Objective: To conduct a meta-analysis of the literature evaluating comparisons on the peri-operative and oncological outcomes between laparoscopic partial nephrectomy (LPN) and laparoscopic ablation therapy (LAT) in the treatment of small renal masses (SRMs).
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The CCND1 G870A gene polymorphism and leukemia or non-Hodgkin lymphoma risk: a meta-analysis.
Asian Pac. J. Cancer Prev.
PUBLISHED: 08-30-2014
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In recent years, mounting evidence has indicated that the CCND1 G870A gene polymorphism, which impacts the mitotic cell cycle, may influence leukemia or non-Hodgkin lymphoma risk. Unfortunately, the previous results were inconsistent. Therefore, a meta-analysis was performed to obtain a more precise estimation of any association. We conducted a search in PubMed, Embase and CNKI covering all published papers up to March, 2014. A total of 9 publications including 10 case-control studies met the inclusion criteria. Odds ratios (ORs) and their 95% confidence intervals (95%CIs) were applied to assess association. The pooled ORs showed significant association in non-Hodgkin lymphoma (comparison A vs G: OR= 1.114, 95%CI=1.053-1.179, p=0.000; homozygote comparison AA vs GG: OR=1.245, 95%CI=1.110-1.396, p=0.000; heterozygote comparison AG vs GG: OR=1.095, 95%CI=1.000-1.199, p=0.05; dominant model AA/GA vs GG: OR=1.137, 95%CI=1.043-1.239, p=0.003; and recessive model AA vs
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Adjuvant and salvage radiotherapy after prostatectomy: a systematic review and meta-analysis.
PLoS ONE
PUBLISHED: 08-14-2014
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In men with adverse prognostic factors (APFs) after radical prostatectomy (RP), the most appropriate timing to administer radiotherapy remains a subject for debate. We conducted a systemic review and meta-analysis to evaluate the therapeutic strategies: adjuvant radiotherapy (ART) and salvage radiotherapy (SRT).
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Follow-up of postpartum women with gestational diabetes mellitus (GDM).
Diabetes Res. Clin. Pract.
PUBLISHED: 08-01-2014
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The incidence of type 2 diabetes in postpartum women with gestational diabetes mellitus (GDM) is high, and blood glucose screening for postpartum women is recommended. Follow-up procedures for women with GDM in the postnatal period need to be improved. We investigated the incidence of GDM and the follow-up of women with GDM in Tianjin, China.
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Electrogenerated chemiluminescence behavior of peptide nanovesicle and its application in sensing dopamine.
Biosens Bioelectron
PUBLISHED: 07-30-2014
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The electrogenerated chemiluminescence (ECL) behavior of the bioinspired peptide nanovesicles (PNVs) was reported for the first time. The PNVs modified glassy carbon electrodes have shown a stable and efficient cathodic ECL signal with K2S2O8 as coreactant in aqueous solution. The possible ECL reaction mechanism was proposed. Dopamine (DA) was chosen as a model analyte to study the potential of the PNVs in the ECL analytical application. It was found that the ECL intensity of the PNVs was effectively increased by trace amounts of DA. The limit of detection was estimated to be 3.15 pM (S/N=3). These results suggest that the PNVs could be a new class of promising materials for the ECL design and bioassays in the future due to their fascinating features, such as excellent biocompatibility, tunable composition as well as capability of molecular recognition.
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ACE2 activity was increased in atherosclerotic plaque by losartan: Possible relation to anti-atherosclerosis.
J Renin Angiotensin Aldosterone Syst
PUBLISHED: 07-30-2014
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Angiotensin-converting enzyme 2 (ACE2) is a new member of the renin-angiotensin system (RAS) and it has been proposed that ACE2 is a potential therapeutic target for the control of cardiovascular disease. The effect of losartan on the ACE2 activity in atherosclerosis was studied.
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Self-assembly and modified luminescence properties of NaY(MoO?)?:Tb³?, Eu³? inverse opals.
Dalton Trans
PUBLISHED: 07-24-2014
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Three-dimensional (3D) inverse opal photonic crystals can not only modulate the emissions of the inserted emitters, they also have the advantage of a large surface to volume ratio, which permits their use in noninvasive fluorescence detection. In this work, novel NaY(MoO4)2:Eu(3+) and NaY(MoO4)2:Tb(3+), Eu(3+) inverse opals were synthesized using a polymethylmethacrylate (PMMA) template by the sol-gel method. It was observed that the photoluminescence (PL) intensity and spontaneous decay rates (SDRs) of the inverse opals were suppressed, in contrast to the corresponding ground reference (REF) samples, due to the modulation of the effective refractive index (n(eff)). The concentration quenching of Eu(3+) and energy transfer from Tb(3+) to Eu(3+) were suppressed, due to the periodic empty cavity structure of the inverse opals.
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Direct measurement of beta-agonists in swine hair extract in multiplexed mode by surface-enhanced raman spectroscopy and microfluidic paper.
Electrophoresis
PUBLISHED: 07-23-2014
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Bare gold nanoparticles selectively enhance the Raman signal of beta-agnonists in swine hair extract at 780 nm, which enables analysis of beta-agonists in swine hair extract without chemical labeling, purification or separation. The analysis is multiplexable and the LOD of beta-agonists is around ng/ml in the assistance of microfluidic paper. This article is protected by copyright. All rights reserved.
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Observation model based on scale interactions for optical flow estimation.
IEEE Trans Image Process
PUBLISHED: 06-27-2014
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In this paper, an original observation model for multiresolution optical flow estimation is introduced. Multiresolution frameworks, often based on coarse-to-fine warping strategies, are widely used by state-of-the-art optical flow methods. They allow the recovery of large motions by successive estimations of the flow field at several resolution levels. Although such approaches perform very efficiently and usually lead to faster minimizations, they generally consider independent problems at each resolution levels and do not exploit the existing interactions between scales (especially the influences of fine scales on larger ones). In this paper, we tackle this issue by proposing a flexible framework, inspired from fluid mechanics, able to partly counter these limitations. For each resolution level, our process filters the equations of interest and decomposes the key variables into resolved (i.e., at a given resolution) and unresolved (i.e., at finer resolutions) components. This enables to derive a new data term that takes into account, at coarse resolutions, the influence of their unresolved parts. From this new term, we propose two different estimation strategies, depending on whether we explicitly know the type of relations between the different scales (as for physical processes) or not. In order to test the efficiency of this new observation model, we have embedded it in a simple multiresolution Lucas-Kanade estimator. Comparing the usual optical flow constraint equation with this new term in the same motion estimation procedure, it clearly appears that the proposed term leads to more consistent estimates and prevents from errors propagation apparition during the estimation. In all situations (synthetic, real, physical images or not), our new term is able to greatly improve the results compared with usual conservation constraints.
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By targeting Stat3 microRNA-17-5p promotes cardiomyocyte apoptosis in response to ischemia followed by reperfusion.
Cell. Physiol. Biochem.
PUBLISHED: 06-23-2014
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Several studies have confirmed the role of microRNAs in regulating ischemia/reperfusion-induced cardiac injury (I/R-I). MiR-17-5p has been regarded as an oncomiR in the development of cancer. However, its potential role in cardiomyocytes has not been exploited. The aim of this study is to investigate the role of miR-17-5p in I/R-I and the underlying mechanism through targeting Stat3, a key surviving factor in cardiomyocytes.
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Suppressing autophagy protects photoreceptor cells from light-induced injury.
Biochem. Biophys. Res. Commun.
PUBLISHED: 06-14-2014
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Autophagy, a conserved cellular self-degradation process, not only serves to protect cells at critical times during development and nutrient stress, but also contributes to cell death. Photoreceptor cells are unique neurons which when directly exposed to the light, transduces light stimuli into visual signal. However, intense light exposure can be cytotoxic to the retina. So far, the precise mechanism underlying retina light injury remains unknown, and the effective therapy is still unavailable. Here, we found that visible light exposure activated the mitogen-activated protein kinases (MAPK) pathway and led to remarkable autophagy in photoreceptor cells (661W cells). Directly blocking autophagy with 3MA or LY294002 markedly attenuated light-induced death in 661W cells. Among the activated downstream factors of MAPK pathway, ERK, not JNK or p-38, played a critical role in light-induced death mechanism. Inhibiting the activation of ERK with its specific inhibitor PD98059 significantly suppressed light-induced autophagy and protected 661W cells from light injury. These results indicate that autophagy is an essential event in light-induced photoreceptor death and that directly blocking autophagy or suppressing autophagy by inhibiting the ERK pathway could effectively attenuates light-induced damage. These observations may have a potential application in the treatment of retinal light injury.
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Efficient energy transfer from inserted CdTe quantum dots to YVO?:Eu³? inverse opals: a novel strategy to improve and expand visible excitation of rare earth ions.
Nanoscale
PUBLISHED: 06-11-2014
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Rare earth (RE)-based phosphors demonstrate sharp emission lines, long lifetimes and high luminescence quantum yields; thus, they have been employed in various photoelectric devices, such as light-emitting diodes (LEDs) and solar spectral converters. However, their applications are largely confined by their narrow excitation bands and small absorption cross sections of 4f-4f transitions. In this paper, we demonstrate a novel strategy to improve and expand the visible excitation bands of Eu(3+) ions through the interface energy transfer (ET) from CdTe quantum dots (QDs) to YVO?:Eu(3+) inverse opal photonic crystals (IOPCs). The significant effects observed in the CdTe QDs/YVO?:Eu(3+) IOPCs composites were that the excitation of Eu(3+) ions was continuously extended from 450 to 590 nm and that the emission intensity of the (5)D?-(7)FJ transitions was enhanced ?20-fold, corresponding to the intrinsic (7)F?-(5)D? excitation at 538 nm. Furthermore, in the IOPC network, the ET efficiency from the QDs to YVO?:Eu(3+) was greatly improved because of the suppression of energy migration among the CdTe QDs, which gave an optimum ET efficiency as high as 47%. Besides, the modulation of photonic stop bands (PSBs) on the radiative transition rates of the QDs and Eu(3+) ions was studied, which showed that the decay lifetime constants for Eu(3+) ions were independent of PSBs, while those of QDs demonstrated a suppression in the PSBs. Their physical nature was explained theoretically.
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New population-based exome data question the pathogenicity of some genetic variants previously associated with Marfan syndrome.
BMC Genet.
PUBLISHED: 06-02-2014
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Marfan syndrome (MFS) is a rare autosomal dominantly inherited connective tissue disorder with an estimated prevalence of 1:5,000. More than 1000 variants have been previously reported to be associated with MFS. However, the disease-causing effect of these variants may be questionable as many of the original studies used low number of controls. To study whether there are possible false-positive variants associated with MFS, four in silico prediction tools (SIFT, Polyphen-2, Grantham score, and conservation across species) were used to predict the pathogenicity of these variant.
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Improved power control using optimal adjustable coefficients for three-phase photovoltaic inverter under unbalanced grid voltage.
ScientificWorldJournal
PUBLISHED: 06-01-2014
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Unbalanced grid faults will lead to several drawbacks in the output power quality of photovoltaic generation (PV) converters, such as power fluctuation, current amplitude swell, and a large quantity of harmonics. The aim of this paper is to propose a flexible AC current generation method by selecting coefficients to overcome these problems in an optimal way. Three coefficients are brought in to tune the output current reference within the required limits of the power quality (the current harmonic distortion, the AC current peak, the power fluctuation, and the DC voltage fluctuation). Through the optimization algorithm, the coefficients can be determined aiming to generate the minimum integrated amplitudes of the active and reactive power references with the constraints of the inverter current and DC voltage fluctuation. Dead-beat controller is utilized to track the optimal current reference in a short period. The method has been verified in PSCAD/EMTDC software.
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The CCND1 G870A gene polymorphism and brain tumor risk: a meta-analysis.
Asian Pac. J. Cancer Prev.
PUBLISHED: 05-30-2014
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In recent years, numerous studies have been performed to investigate the CCND1 G870A gene polymorphism impact on brain tumors susceptibility. Unfortunately, the results of previous studies were inconsistent. Therefore, we performed a meta-analysis to derive a more precise estimation of any association.
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Biochar from Alternanthera philoxeroides could remove Pb(II) efficiently.
Bioresour. Technol.
PUBLISHED: 05-24-2014
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A novel bio-adsorbent was successfully synthesized by pyrolyzing Alternanthera philoxeroides (AP), one of the most widely used hydrophytes for eutrophic lake ecological restoration under O2-limited condition at 600 °C. Compared with commercially active carbon (AC), the initial solution pH had a weak effect on the adsorption of Pb(II) by AP biochar (APB). The maximum adsorption capacity of APB for Pb(II) was 257.12 mg/g, which was 5.3 times of that of the AC. The adsorption process was fast, with only 2.5h to reach adsorption equilibrium. The adsorption mechanism of Pb(II) by APB involves the precipitation and complexation of Pb(II) with free carboxyl/hydroxyl functional groups and mineral carbonates of APB as well as ion replacement between Pb(II) and alkaline earth cations. These results suggest that using a low-cost APB adsorbent for heavy metals contaminated water treatment may have great ecological and environmental significance.
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Clonal dissemination of multilocus sequence type 11 Klebsiella pneumoniae carbapenemase - producing K. pneumoniae in a Chinese teaching hospital.
APMIS
PUBLISHED: 05-23-2014
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Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae has disseminated rapidly in China. We aimed to analyze the molecular epidemiology of four KPC-producing K. pneumoniae strains isolated from a suspected clonal outbreak during a 3-month period and to track the dissemination of KPC-producing K. pneumonia retrospectively. We created antimicrobial susceptibility profiles using an automated broth microdilution system and broth microdilution methods. We screened carbapenemase and KPC phenotypes using the modified Hodge test and meropenem-boronic acid (BA) disk test, respectively. We identified ?-lactamase genes with PCR and sequencing. We investigated clonal relatedness for epidemiological comparison using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). All isolates expressed multidrug resistance and yielded positive results for the modified Hodge and meropenem-BA disk tests. The isolates all carried blaKPC -2 , and coproduced CTX-M-type extended-spectrum ?-lactamase. PFGE and MLST showed that the isolates were clonally related. The PFGE patterns of these isolates had ?90% similarity. We found a single clone, sequence type (ST) 11, and its typical dissemination mode resembled clonal spread. The dissemination of KPC-producing K. pneumoniae is clonally related and there is probable local transmission of a successful ST11 clone.
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IL-17 regulates the expressions of RANKL and OPG in human periodontal ligament cells via TRAF6/TBK1-JNK/NF-?B pathways.
Immunology
PUBLISHED: 05-03-2014
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Interleukin-17 (IL-17 or IL-17A), a pleiotropic cytokine produced by T helper (Th) 17 cells, is involved in the pathogenesis of various autoimmune and inflammatory disorders, including periodontitis. Although the ability of pro-inflammation in periodontitis have been widely investigated, the other biological functions of IL-17, including its role in bone remodeling and the underlying molecular mechanism, have not been well clarified. In the present study, IL-17 could significantly enhance the expression of receptor activator for nuclear factor-?B ligand (RANKL) and inhibit the expression of osteoprotegerin (OPG) in human periodontal ligament cells (hPDLCs), the two critical indicators for osteoclastogenesis, suggesting IL-17 may play a destructive role in the pathogenesis of periodontal bone remodeling. Pharmaceutical signal inhibitors targeted at MAPKs, Akt or NF-?B signals, inhibited IL-17-induced RANKL and OPG regulation. Notably, the enhancement of RANKL was significantly blocked by the inhibitors of JNK and NF-?B signals. The upstream signals were further investigated with the small interfering RNA (siRNA). Both TRAF6 and TBK1 were found to be the critically signal molecules for IL-17-dependent RANKL regulation in hPDLCs. These findings may provide comprehensive understanding of the role of IL-17 in the pathogenesis of periodontitis and might also provide a reasonable way for periodontitis therapy. This article is protected by copyright. All rights reserved.
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Histologic comparison between platelet-rich plasma and blood clot in regenerative endodontic treatment: an animal study.
J Endod
PUBLISHED: 05-02-2014
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In regenerative endodontic treatment (RET) for immature permanent tooth, better treatment results could be obtained by applying platelet-rich plasma (PRP) as the scaffold rather than the blood clot. The goal of this study was to compare the histologic differences between using PRP and blood clot in RET.
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Thermogravimetric analysis of atomized ferromagnetic composites with multiwalled carbon nanotubes: an unusual behavior of nickel in nanospace.
J Nanosci Nanotechnol
PUBLISHED: 04-22-2014
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A spin polarization of atomized ferromagnetic atoms like cobalt or nickel in nano space results in the modification of the electron configuration in the ferromagnetic atom that changes its oxidative property. We have prepared cobalt and nickel composites with multiwalled carbon nanotubes using atomized cobalt and nickel particles, for investigating their thermal oxidative behavior by thermogravimetric analysis (TGA). The composites showed the absence of a thermal oxidation in the temperature range of ambient to the break down temperature of multiwalled carbon nanotubes at 800 degrees C. At this temperature while Co composite forms cobalt oxide, the Ni composite becomes volatile that results in the divergent behavior of the two ferromagnetic compounds with a weight gain observed in TGA for Co and a loss for Ni. The mechanisms operating in the two cases are discussed in this work.
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Prostaglandin E2 EP1 receptor enhances TGF??1-induced mesangial cell injury.
Int. J. Mol. Med.
PUBLISHED: 04-20-2014
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Increasing evidence indicates that transforming growth factor-?1 (TGF-?1) is a pivotal mediator in the pathogenesis of renal fibrosis. Mesangial cells (MCs) are important for glomerular function under both physiological and pathological conditions. Studies have found that the expression level of prostaglandin E2 (PGE2) in MCs increases under high glucose conditions, that PGE2 affects the proliferation and hypertrophy of MCs mainly through the EP1 pathway, and that the proliferation of MCs and the accumulation of extracellular matrix are the main events leading to glomerular fibrosis. In this study, we investigated the effects and mechanisms of action of the EP1 receptor, which is induced by transforming growth factor (TGF)-?1, on the proliferation of mouse MCs, the accumulation of extracellular matrix and the expression of PGE2 synthase. Primary mouse glomerular MCs were isolated from EP1 receptor-deficient mice (EP1-/- mice, in which the EP1 receptor was knocked down) and wild-type (WT) mice (WT MCs). In our preliminary experiments, we found that cell proliferation, as well as the mRNA and protein expression of cyclin D1, proliferating cell nuclear antigen (PCNA), fibronectin (FN), collagen ? (Col?), membrane-associated PGE2 synthase-1 (mPGES-1) and cyclooxygenase-2 (COX-2) in the WT MCs were significantly increased following treatment with 10 ng/ml TGF-?1 for 24 h. Compared with the WT MCs, following the knockdown of the EP1 gene, the TGF-?1-induced MC injury was markedly suppressed. The aforementioned changes were notably enhanced following treatment with the EP1 agonist, 17-phenyl trinor PGE2 ethyl amide. Additionally, TGF-?1 induced extracellular signal-regulated kinase (ERK) phosphorylation. We found that the TGF-?1-induced ERK phosphorylation was alleviated by EP1 knockdown and promoted by EP1 expresion. These results suggest that the EP1 receptor plays a role in the proliferation of mouse MCs, in the accumulation of extracellular matrix and in the expression of mPGES-1 induced by TGF-?1. Its mechanisms of action are possibly related to the reinforcement of ERK phosphorylation.
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Modelization of nanospace interaction involving a ferromagnetic atom: a spin polarization effect study by thermogravimetric analysis.
J Nanosci Nanotechnol
PUBLISHED: 04-17-2014
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Ab initio studies of ferromagnetic atom interacting with carbon nanotubes have been reported in the literature that predict when the interaction is strong, a higher hybridization with confinement effect will result in spin polarization in the ferromagnetic atom. The spin polarization effect on the thermal oxidation to form its oxide is modeled here for the ferromagnetic atom and its alloy, as the above studies predict the 4s electrons are polarized in the atom. The four models developed here provide a pathway for distinguishing the type of interaction that exists in the real system. The extent of spin polarization in the ferromagnetic atom has been examined by varying the amount of carbon nanotubes in the composites in the thermogravimetric experiments. In this study we report the experimental results on the CoNi alloy which appears to show selective spin polarization. The products of the thermal oxidation has been analyzed by Fourier Transform Infrared Spectroscopy.
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Effects of anticipation on perception of facial expressions.
Percept Mot Skills
PUBLISHED: 04-15-2014
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Human beings do not passively perceive the facial expressions of other people, but predict observed facial expressions by employing past experiences. The aim of the current study was to investigate whether and how anticipation affected the perception of facial expressions. A 3-way repeated-measures ANOVA on anticipation, orientation, and facial expression was performed on RTs and recognition accuracy in Experiments 1 and 2. The results showed that anticipation reduced susceptibility to negative facial expressions. In this regard, anticipation might be considered as an effective emotion-regulation strategy. In addition, a decreased inversion effect for positive facial expressions was found in the predictable condition, which might reflect a switch from feature-based to holistic processing.
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Human sex differences in emotional processing of own-race and other-race faces.
Neuroreport
PUBLISHED: 04-02-2014
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There is evidence that women and men show differences in the perception of affective facial expressions. However, none of the previous studies directly investigated sex differences in emotional processing of own-race and other-race faces. The current study addressed this issue using high time resolution event-related potential techniques. In total, data from 25 participants (13 women and 12 men) were analyzed. It was found that women showed increased N170 amplitudes to negative White faces compared with negative Chinese faces over the right hemisphere electrodes. This result suggests that women show enhanced sensitivity to other-race faces showing negative emotions (fear or disgust), which may contribute toward evolution. However, the current data showed that men had increased N170 amplitudes to happy Chinese versus happy White faces over the left hemisphere electrodes, indicating that men show enhanced sensitivity to own-race faces showing positive emotions (happiness). In this respect, men might use past pleasant emotional experiences to boost recognition of own-race faces.
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[Investigation of antibacterial activity of topical antimicrobials against methicillin-resistant Staphylococcus aureus].
Zhonghua Shao Shang Za Zhi
PUBLISHED: 04-02-2014
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To investigate the antibacterial activity of silver sulfadiazine (SD-Ag), mupirocin, and clotrimazole used alone or in combination against methicillin-resistant Staphylococcus aureus (MRSA) isolated from burn wounds.
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Inducible nitric oxide synthase inhibition reverses pulmonary arterial dysfunction in lung transplantation.
Inflamm. Res.
PUBLISHED: 03-31-2014
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Ischemia-reperfusion injury (IRI) after lung transplantation remains a significant cause of morbidity and mortality. Lung IRI induces nitric oxide synthesis (iNOS) and reactive nitrogen species, decreasing nitric oxide bioavailability. We hypothesized that ischemia-induced iNOS intensifies with reperfusion and contributes to IRI-induced pulmonary arterial regulatory dysfunction, which may lead to early graft failure and cause pulmonary edema. The aim of this study was to determine whether ischemia-reperfusion alters inducible and endothelial nitric oxide synthase expression, potentially affecting pulmonary perfusion. We further evaluated the role of iNOS in post-transplantation pulmonary arterial disorder.
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Ag-SiO?-Er?O? nanocomposites: highly effective upconversion luminescence at high power excitation and high temperature.
Sci Rep
PUBLISHED: 03-25-2014
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Rare Earth (RE) activated upconversion phosphors (UCPs), have demonstrated significant application potentials in some front fields, including solar energy conversion and bio-application. However, some bottleneck problems should be overcame, such as the lower upconversion efficiency, narrower excitation band, concentration-quenching and temperature-quenching. To solve these problems, the Ag-SiO2-Er2O3 nanocomposites were fabricated, in which the upconversion luminescence (UCL) of Er2O3 was white broadband. Through the interaction of Er2O3 with surface plasmon (SP) of silver nanoparticles (SNPs), the threshold power for generating broadbands was suppressed largely in contrast to the Er2O3 nanoparticles (NPs), while the UCL brightness was enhanced remarkably, ranging from several to 10(4) times, which strongly depended on the power density of excitation light. At excitation power density of 1.50?W/mm(2) of 980?nm light, the UCL intensity of Ag-SiO2-Er2O3 is 40-folds than the well-known NaYF4:Yb(3+),Er(3+) commercial powders. And more, it is also interesting to observe that the composites demonstrate two excitation bands extending of 780-980?nm, highly improved UCL with elevated temperature and excitation power density. The UCL mechanism related to UCL enhancement was carefully studied.
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Computer-aided diagnosis expert system for cerebrovascular diseases.
Neurol. Res.
PUBLISHED: 03-20-2014
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To establish an expert diagnosis system for cerebrovascular diseases (CVDs) and assess accuracy of the diagnosis system.
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Complete genome sequence of a novel infectious bronchitis virus strain circulating in China with a distinct S gene.
Virus Genes
PUBLISHED: 03-14-2014
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An avian infectious bronchitis virus (IBV) was isolated and identified from a commercial layer flock vaccinated with live attenuated H120 vaccine in China, designed as ck/CH/IBTZ/2012. To determine the origination and evolution of this isolated strain, we have carried out a complete genome sequencing of this strain. The genome of the ck/CH/IBTZ/2012 strain is 27,691 nucleotides in length and includes more than 10 open reading frames. Sequence comparison and phylogenetic analysis based on the full-length genomic sequences showed that ck/CH/IBTZ/2012 is mostly related to the LX4-like strains. However, sequence analysis based on the spike protein (S) gene sequences revealed that ck/CH/IBTZ/2012 possesses a distinct S gene setting it apart from the Massachusetts-type strains and LX4-type strains. The cleavage site within the spike protein (S) of ck/CH/IBTZ/2012 is HRRKR, which is different from the majority of the IBVs in China for their cleavage sits are HRRRR. Recombination analysis showed that ck/CH/IBTZ/2012 is a chimeric virus with a LX4-like backbone except S gene which might be from an unknown strain. Based on the data presented in this paper, it can be concluded that genetic changes due to adaptive evolution and recombination both contributed to the origin of strain ck/CH/IBTZ/2012, which belongs to a new genotype.
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Mutant Gq/11 promote uveal melanoma tumorigenesis by activating YAP.
Cancer Cell
PUBLISHED: 03-09-2014
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Uveal melanoma (UM) is the most common cancer in adult eyes. Approximately 80% of UMs harbor somatic activating mutations in GNAQ or GNA11 (encoding Gq or G11, respectively). Herein, we show in both cell culture and human tumors that cancer-associated Gq/11 mutants activate YAP, a major effector of the Hippo tumor suppressor pathway that is also regulated by G protein-coupled receptor signaling. YAP mediates the oncogenic activity of mutant Gq/11 in UM development, and the YAP inhibitor verteporfin blocks tumor growth of UM cells containing Gq/11 mutations. This study reveals an essential role of the Hippo-YAP pathway in Gq/11-induced tumorigenesis and suggests YAP as a potential drug target for UM patients carrying mutations in GNAQ or GNA11.
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Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling.
Science
PUBLISHED: 03-01-2014
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Auxin-binding protein 1 (ABP1) was discovered nearly 40 years ago and was shown to be essential for plant development and morphogenesis, but its mode of action remains unclear. Here, we report that the plasma membrane-localized transmembrane kinase (TMK) receptor-like kinases interact with ABP1 and transduce auxin signal to activate plasma membrane-associated ROPs [Rho-like guanosine triphosphatases (GTPase) from plants], leading to changes in the cytoskeleton and the shape of leaf pavement cells in Arabidopsis. The interaction between ABP1 and TMK at the cell surface is induced by auxin and requires ABP1 sensing of auxin. These findings show that TMK proteins and ABP1 form a cell surface auxin perception complex that activates ROP signaling pathways, regulating nontranscriptional cytoplasmic responses and associated fundamental processes.
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Co-expression of phosphoenolpyruvate carboxykinase and nicotinic acid phosphoribosyltransferase for succinate production in engineered Escherichia coli.
Enzyme Microb. Technol.
PUBLISHED: 02-26-2014
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Succinate is not the dominant fermentation product from xylose in wild-type Escherichia coli K12. E. coli BA 203 is a lactate dehydrogenase (ldhA), pyruvate formate lyase (pflB), and phosphoenolpyruvate (PEP)-carboxylase (ppc) deletion strain. To increase succinate accumulation and reduce byproduct formation, engineered E. coli BA204, in which ATP-forming PEP-carboxykinase (PEPCK) is overexpressed in BA203, was constructed and produced 2.17-fold higher succinate yield. To further improve the biomass and the consumption rate of xylose, nicotinic acid phosphoribosyltransferase (NAPRTase), a rate limiting enzyme in the synthesis of NAD(H), was also overexpressed. Thus, co-expression of PEPCK and NAPRTase in recombinant E. coli BA209 was investigated. In BA209, the pck gene and the pncB gene each have a trc promoter, hence, both genes are well expressed. During a 72-h anaerobic fermentation in sealed bottles, the total concentration of NAD(H) in BA209 was 1.25-fold higher than that in BA204, and the NADH/NAD+ ratio decreased from 0.28 to 0.11. During the exclusively anaerobic fermentation in a 3-L bioreactor, BA209 consumed 17.1 g L?¹ xylose and produced 15.5 g L?¹ succinate. Furthermore, anaerobic fermentation of corn stalk hydrolysate contained 30.1 g L?¹ xylose, 2.1 g L?¹ glucose and 1.5 g L?¹ arabinose, it produced a final succinate concentration of 17.2 g L?¹ with a yield of 0.94 g g?¹ total sugars.
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The alkaloid matrine of the root of Sophora flavescens prevents arrhythmogenic effect of ouabain.
Phytomedicine
PUBLISHED: 02-22-2014
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Matrine, a alkaloid of the root of Sophora flavescens, has multiple protective effects on the cardiovascular system including cardiac arrhythmias. However, the molecular and ionic mechanisms of matrine have not been well investigated. Our study aimed at to shed a light on the issue to investigate the antiarrhythmic effects of matrine by using ouabain to construct an arrhythmic model of cardiomyocytes. In this experiment, matrine significantly and dose-dependently increased the doses of ouabain required to induce cardiac arrhythmias and decreased the duration of arrhythmias in guinea pigs. In cardiomyocytes of guinea pigs, ouabain 10 ?M prolonged action potential duration by 80% (p<0.05) and increased L-type Ca(2+) currents and Ca(2+) transients induced by KCl (p<0.05). Matrine 100 ?M shortened the prolongation of APD and prevented the increase of L-type Ca(2+) currents and Ca(2+) transients induced by ouabain. Taken together, these findings provide the first evidence that matrine possessed arrhythmogenic effect of ouabain by inhibiting of L-type Ca(2+) currents and Ca(2+) overload in guinea pigs.
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En bloc transurethral resection with 2-micron continuous-wave laser for primary non-muscle-invasive bladder cancer: a randomized controlled trial.
World J Urol
PUBLISHED: 02-20-2014
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To evaluate the practicability of en bloc transurethral resection with 2-micron continuous-wave laser as treatment for primary non-muscle-invasive bladder cancer (NMIBC).
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Mismatch of AO anatomically shaped distal humeral plate with humeral shaft forward flexion angulation in adult Chinese population.
Eur J Orthop Surg Traumatol
PUBLISHED: 02-19-2014
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LCP extra-articular distal humerus plate (LCPEA) designed by AO has been introduced as an anatomically shaped plate that improves the results of surgical fixation in extra-articular distal humeral shaft fractures. However, no study analyzed whether LCPEA matches humeral shaft forward flexion angulation (FFA).
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Aberrant expression of CD227 is correlated with tumor characteristics and invasiveness of breast carcinoma.
J. Cancer Res. Clin. Oncol.
PUBLISHED: 02-17-2014
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Increasing evidences demonstrate that CD227 plays a crucial role in the development and progression of breast cancer. However, the function of CD227 in breast carcinoma was still controversial and the investigation on CD227 in Asian race was scarce.
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Target cell extraction coupled with LC-MS/MS analysis for screening potential bioactive components in Ginkgo biloba extract with preventive effect against diabetic nephropathy.
Biomed. Chromatogr.
PUBLISHED: 01-27-2014
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A rapid and useful approach for screening potential bioactive components in Ginkgo biloba extract (GBE) with preventive effect against diabetic nephropathy (DN) was developed using mesangial cells extraction coupled with high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. Mesangial cells were first divided into two groups according to their treatments with high glucose or high glucose plus GBE. After incubation for 4, 8, 12, 16, 24 and 48?h, the cells were harvested and extracted with 40% acetic acid in water before LC-MS/MS analysis. Then, 19 compounds and five metabolites were found to selectively combine with mesangial cells. Notably, compounds including quercetin and rutin were identified or tentatively characterized according to the results of retention time and MS spectra, which is highly consistent with our previous reports that quercetin and rutin are potent protective agents against glomerulosclerosis in DN. Therefore, all these results indicate that target cell extraction coupled with LC-MS/MS analysis can be successfully applied for predicting the bioactive components in GBE with preventive effect against DN. Copyright © 2014 John Wiley & Sons, Ltd.
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Rho-GTPase-regulated vesicle trafficking in plant cell polarity.
Biochem. Soc. Trans.
PUBLISHED: 01-24-2014
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ROPs (Rho of plants) belong to a large family of plant-specific Rho-like small GTPases that function as essential molecular switches to control diverse cellular processes including cytoskeleton organization, cell polarization, cytokinesis, cell differentiation and vesicle trafficking. Although the machineries of vesicle trafficking and cell polarity in plants have been individually well addressed, how ROPs co-ordinate those processes is still largely unclear. Recent progress has been made towards an understanding of the co-ordination of ROP signalling and trafficking of PIN (PINFORMED) transporters for the plant hormone auxin in both root and leaf pavement cells. PIN transporters constantly shuttle between the endosomal compartments and the polar plasma membrane domains, therefore the modulation of PIN-dependent auxin transport between cells is a main developmental output of ROP-regulated vesicle trafficking. The present review focuses on these cellular mechanisms, especially the integration of ROP-based vesicle trafficking and plant cell polarity.
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MicroRNA-328 as a regulator of cardiac hypertrophy.
Int. J. Cardiol.
PUBLISHED: 01-22-2014
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Cardiac hypertrophy is a primary predictor of progressive heart disease that often results in heart failure. Growing evidence has demonstrated that microRNAs (miRNAs) play a critical role in regulating cardiac hypertrophy. This study was designed to evaluate the effect of miR-328 on cardiac hypertrophy and the potential molecular mechanisms. We found that transgenic overexpression of miR-328 in the heart induced cardiac hypertrophy in mice, which was accompanied by reduced SERCA2a level increased intracellular calcium concentration and calcineurin protein level, and enhanced NFATc3 nuclear translocation. However, normalization of miR-328 level by its antisense chemically modified with locked nucleic acid (LNA-antimiR-328) reversed the changes. Forced expression of miR-328 resulted in cardiomyocyte hypertrophy in cultured neonatal rat ventricular cells, which was accompanied by downregulation of SERCA2a expression and activation of the calcineurin/NFATc3 signaling pathway. These changes were abolished by LNA-antimiR-328. We validated the SERCA2a as a direct target for miR-328. MiR-328 expression was upregulated in cardiomyocyte treated with isoproterenol (ISO) to induce hypertrophy; while knockdown of miR-328 attenuated the hypertrophic responses. The level of miR-328 was significantly elevated in a mouse model of hypertrophy by thoracic aortic banding (TAC). Consistently, SERCA2a was downregulated, whereas calcineurin were upregulated, and NFATc3 nuclear translocation was enhanced. In contrast, hypertrophy in these mice was significantly alleviated when treated with miR-328 antisense. MiR-328 promotes cardiac hypertrophy by targeting SERCA2a. Our study therefore uncovered a novel molecular mechanism for cardiac hypertrophy and indicated miR-328 as a potential therapeutic target for this cardiac condition.
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Combination of fluconazole with non-antifungal agents: a promising approach to cope with resistant Candida albicans infections and insight into new antifungal agent discovery.
Int. J. Antimicrob. Agents
PUBLISHED: 01-22-2014
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The past decades have witnessed a dramatic increase in invasive fungal infections, especially candidiasis. Despite the development of more effective new antifungal agents, fluconazole (FLC) is still widely used in the clinic because of its efficacy and low toxicity. However, as the number of patients treated with FLC has increased, FLC-resistant Candida albicans isolates emerge more frequently. In addition, biofilm-associated infections are commonly encountered and their resistance poses a great challenge to antifungal treatment. Various approaches have been proposed to increase the susceptibility of C. albicans to FLC in order to cope with treatment failures, among which is the combination of FLC with different classes of non-antifungal agents such as antibacterials, calcineurin inhibitors, heat shock protein 90 inhibitors, calcium homeostasis regulators and traditional Chinese medicine drugs. Interestingly, many of these combinations showed synergistic effects against C. albicans, especially resistant strains. The main mechanisms of these synergistic effects appear to be increasing the permeability of the membrane, reducing the efflux of antifungal drugs, interfering with intracellular ion homeostasis, inhibiting the activity of proteins and enzymes required for fungal survival, and inhibiting biofilm formation. These modes of action and the antifungal mechanisms of various compounds referenced in this paper highlight the idea that the reversal of fungal resistance can be achieved through various mechanisms. Studies examining drug interactions will hopefully provide new approaches against antifungal drug resistance as well as insight into antifungal agent discovery.
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The expression and role of tyrosine kinase ETK/BMX in renal cell carcinoma.
J. Exp. Clin. Cancer Res.
PUBLISHED: 01-07-2014
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Expression of the non-receptor tyrosine kinase ETK/BMX has been reported in several solid tumors, but the underlying molecular mechanisms and its clinical significance in renal cell carcinoma (RCC) remain to be elucidated.
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An Auxin-Mediated Shift toward Growth Isotropy Promotes Organ Formation at the Shoot Meristem in Arabidopsis.
Curr. Biol.
PUBLISHED: 01-06-2014
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To control morphogenesis, molecular regulatory networks have to interfere with the mechanical properties of the individual cells of developing organs and tissues, but how this is achieved is not well known. We study this issue here in the shoot meristem of higher plants, a group of undifferentiated cells where complex changes in growth rates and directions lead to the continuous formation of new organs [1, 2]. Here, we show that the plant hormone auxin plays an important role in this process via a dual, local effect on the extracellular matrix, the cell wall, which determines cell shape. Our study reveals that auxin not only causes a limited reduction in wall stiffness but also directly interferes with wall anisotropy via the regulation of cortical microtubule dynamics. We further show that to induce growth isotropy and organ outgrowth, auxin somehow interferes with the cortical microtubule-ordering activity of a network of proteins, including AUXIN BINDING PROTEIN 1 and KATANIN 1. Numerical simulations further indicate that the induced isotropy is sufficient to amplify the effects of the relatively minor changes in wall stiffness to promote organogenesis and the establishment of new growth axes in a robust manner.
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Emotional body-word conflict evokes enhanced n450 and slow potential.
PLoS ONE
PUBLISHED: 01-01-2014
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Emotional conflict refers to the influence of task irrelevant affective stimuli on current task set. Previously used emotional face-word tasks have produced certain electrophysiological phenomena, such as an enhanced N450 and slow potential; however, it remains unknown whether these effects emerge in other tasks. The present study used an emotional body-word conflict task to investigate the neural dynamics of emotional conflict as reflected by response time, accuracy, and event-related potentials, which were recorded with the aim of replicating the previously observed N450 and slow potential effect. Results indicated increased response time and decreased accuracy in the incongruent condition relative to the congruent condition, indicating a robust interference effect. Furthermore, the incongruent condition evoked pronounced N450 amplitudes and a more positive slow potential, which might be associated with conflict-monitoring and conflict resolution. The present findings extend our understanding of emotional conflict to the body-word domain.
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Clinicopathological significance of microRNA-214 in gastric cancer and its effect on cell biological behaviour.
PLoS ONE
PUBLISHED: 01-01-2014
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Accumulating evidence indicates that numerous microRNAs are involved in the tumorigenesis and progression of gastric cancer, while the clinical significance of microRNA-214 in gastric cancer is poorly understood and the exact role of microRNA-214 in gastric cancer remains obscure. In the present study, expression levels of microRNA-214 in 80 gastric carcinoma tissues, 18 nontumourous gastric tissues, and 4 types of gastric cancer cell lines were quantified by reverse transcription followed by real-time quantitative polymerase chain reaction (RT-qPCR), and the relationship between microRNA-214 expression and cliniopathological characteristics including prognosis was explored. To investigate the potential role of microRNA-214 in gastric cancer cell biological behaviour, we performed cell proliferation, apoptosis, migration and invasion assays in four gastric cancer cell lines and an immortalized gastric cell line in vitro. Our results showed that microRNA-214 was dramatically downregulated in gastric cancer tissues and gastric cancer cell lines, compared with nontumourous gastric tissues. Stepwise downregulation of microRNA-214 expression was observed among nontumourous gastric mucosa, nonmetastasis gastric cancer tissues, and metastasis gastric cancer tissues. The expression of microRNA-214 was significantly inversely correlated with lymph node metastasis and tumour size but had no correlation with the patient's prognosis. Ectopic expression of microRNA-214 could inhibit cell migration and invasion ability in SGC7901 and MKN45 gastric cancer cells. And knockdown of microRNA-214 significantly facilitated cell proliferation, migration and invasion in a cell-specific manner in MKN28, BGC823 and GES-1 cells. Colony stimulating factor 1 (CSF1) was identified as a target gene of microRNA-214. In summary, our data demonstrated that microRNA-214 is a promising novel biomarker for lymph node metastasis in patients with gastric cancer. And we identified that downregulation of microRNA-214 may regulate the proliferation, invasion and migration of gastric cancer cells by directly targeting CSF1.
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WBSA: web service for bisulfite sequencing data analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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Whole-Genome Bisulfite Sequencing (WGBS) and genome-wide Reduced Representation Bisulfite Sequencing (RRBS) are widely used to study DNA methylation. However, data analysis is complicated, lengthy, and hampered by a lack of seamless analytical pipelines. To address these issues, we developed a convenient, stable, and efficient web service called Web Service for Bisulfite Sequencing Data Analysis (WBSA) to analyze bisulfate sequencing data. WBSA focuses on not only CpG methylation, which is the most common biochemical modification in eukaryotic DNA, but also non-CG methylation, which have been observed in plants, iPS cells, oocytes, neurons and stem cells of human. WBSA comprises three main modules as follows: WGBS data analysis, RRBS data analysis, and differentially methylated region (DMR) identification. The WGBS and RRBS modules execute read mapping, methylation site identification, annotation, and advanced analysis, whereas the DMR module identifies actual DMRs and annotates their correlations to genes. WBSA can be accessed and used without charge either online or local version. WBSA also includes the executables of the Portable Batch System (PBS) and standalone versions that can be downloaded from the website together with the installation instructions. WBSA is available at no charge for academic users at http://wbsa.big.ac.cn.
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XRCC3 C18067T polymorphism contributes a decreased risk to both basal cell carcinoma and squamous cell carcinoma: evidence from a meta-analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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The X-ray repair cross-complementing group 3 (XRCC3) in homologous recombination repair (HRR) pathway plays a very important role in DNA double-strand break repair (DSBR). Variations in the XRCC3 gene might lead to altered protein structure or function which may change DSBR efficiency and result in cancer. The XRCC3 C18067T polymorphism has been reported to be associated with skin cancer susceptibility, yet the results of these previous results have been inconsistent or controversial. To derive a more precise estimation of the association, we conducted a meta-analysis.
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Synthesis of Heteroaryl Compounds through Cross-Coupling Reaction of Aryl Bromides or Benzyl Halides with Thienyl and Pyridyl Aluminum Reagents.
J. Org. Chem.
PUBLISHED: 12-11-2013
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An efficient method for synthesis of useful biaryl building blocks containing 2-thienyl, 3-thienyl, 2-pyridyl, and 3-pyridyl moieties was provided through cross-coupling reactions of aryl bromides or benzyl halides with heteroaryl aluminum reagents in the presence of Pd(OAc)2 and (o-tolyl)3P. The coupling reaction also worked efficiently with heteroaryl bromides affording series of heterobiaryl compounds. The reaction of phenylbromide with in situ prepared 3-pyridyl aluminum was demonstrated to afford the product 8a in high yield. Additionally, the catalytic system was also suited well for the coupling reaction of benzyl halides with pyridyl aluminum reagents to afford series of pyridyl-arylmethane.
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[Cumulative analgesic effects of EA stimulation of sanyinjiao (SP 6) in primary dysmenorrhea patients: a multicenter randomized controlled clinical trial].
Zhen Ci Yan Jiu
PUBLISHED: 12-07-2013
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To compare the cumulative analgesic effect of electroacupuncture (EA) stimulation of Sanyinjiao (SP 6), Xuanzhong (GB 39) and non-acupoint for primary dysmenorrhea patients.
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A Novel Method for Real-Time Atrial Fibrillation Detection in Electrocardiograms Using Multiple Parameters.
Ann Noninvasive Electrocardiol
PUBLISHED: 11-20-2013
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Automatic detection of atrial fibrillation (AF) in electrocardiograms (ECGs) is beneficial for AF diagnosis, therapy, and management. In this article, a novel method of AF detection is introduced. Most current methods only utilize the RR interval as a critical parameter to detect AF; thus, these methods commonly confuse AF with other arrhythmias.
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A portable hydro-thermo-mechanical loading cell for in situ small angle neutron scattering studies of proton exchange membranes.
Rev Sci Instrum
PUBLISHED: 11-05-2013
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A portable hydro-thermo-mechanical loading cell has been designed to enable in situ small angle neutron scattering (SANS) studies of proton exchange membranes (PEMs) under immersed tensile loadings at different temperatures. The cell consists of three main parts as follows: a letter-paper-size motor-driven mechanical load frame, a SANS friendly reservoir that provides stable immersed and thermal sample conditions, and a data acquisition and control system. The ex situ tensile tests of Nafion 212 membranes demonstrated a satisfactory thermo-mechanical testing performance of the cell for either dry or immersed conditions at elevated temperatures. The in situ SANS tensile measurements on the Nafion 212 membranes immersed in D2O at 70 °C proved the feasibility and capability of the cell for small angle scattering study on deformation behaviors of PEM and other polymer materials under hydro-thermo-mechanical loading.
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High performance rotational vibration isolator.
Rev Sci Instrum
PUBLISHED: 11-05-2013
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We present a new rotational vibration isolator with an extremely low resonant frequency of 0.055 ± 0.002 Hz. The isolator consists of two concentric spheres separated by a layer of water and joined by very soft silicone springs. The isolator reduces rotation noise at all frequencies above its resonance which is very important for airborne mineral detection. We show that more than 40 dB of isolation is achieved in a helicopter survey for rotations at frequencies between 2 Hz and 20 Hz. Issues affecting performance such as translation to rotation coupling and temperature are discussed. The isolator contains almost no metal, making it particularly suitable for electromagnetic sensors.
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[The peoples mentality confronting plague in the Ming Dynasty].
Zhonghua Yi Shi Za Zhi
PUBLISHED: 10-19-2013
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The social influence of plague was not only confined to its pathogenicity, but also its close relationship with the peoples mentality. According to the historical materials of the Ming Dynasty, there were 2 kinds of mentalities when confronting with the prevalence of plague: negative and positive. The former included fear, helplessness, depression and superstition etc., and the latter included intelligence, consolation, thanksgiving and vigour etc. The negative passive mentality didnt help to fight effectively against the plague, or might even aggravate its prevalence. However, the positive mentality helped ameliorate and control the plague, and also the rehabilitation of the order of production and living order after the plague.
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[Relationship between tic symptom severity and amplitude of low frequency fluctuation of resting-state functional magnetic resonance imaging of Tourette syndrome].
Zhonghua Er Ke Za Zhi
PUBLISHED: 10-15-2013
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To examine the relationship between tic symptom severity and amplitude of low frequency fluctuation (ALFF) brain functioning of the first-episode Tourette syndrome through resting-state functional magnetic resonance imaging (fMRI).
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[Expression of LIM mineralization protein-1 in the apical papilla and dental pulp tissues of human teeth].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 10-15-2013
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To examine the expression of LIM mineralization protein-1 (LMP-1) in the apical papilla and dental pulp tissues of human immature permanent teeth and to investigate the role of LMP-1 in the development and maturation of pulp-dentin complex.
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Knockdown of Bmi1 inhibits the stemness properties and tumorigenicity of human bladder cancer stem cell-like side population cells.
Oncol. Rep.
PUBLISHED: 09-22-2013
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B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi1) is directly involved in cell growth, proliferation and self-renewal of cancer stem cells (CSCs). The aim of the present study was to assess the role of Bmi1 in the maintenance of stemness properties and tumorigenicity of human bladder CSC-like side population (SP) cells. SP cells were sorted by flow cytometry using Hoechst 33342 staining. Bmi1 mRNA and protein expression in SP and non-SP (NSP) cells was analyzed by quantitative PCR, immunofluorescence and western blotting. The stemness properties of SP cells included cell proliferation, migration, self-renewal, chemotherapy resistance and cell cycle progression were assessed. Tumor formation was also assessed in human bladder cancer xenografts after Bmi1 silencing. The mRNA expression of Bmi1 was upregulated in SP cells when compared with that in the NSP cells. Knockdown of Bmi1 in SP cells resulted in inhibition of cell proliferation, migration and tumor sphere formation, enhanced sensitivity to cisplatin, and cell cycle arrest in the G0/G1 phase. Bmi1 knockdown inhibited cell cycle progression through derepression of the p16INK4a/p14ARF locus. Bmi1-siRNA SP cells failed to produce tumors in recipient mice, while typical urothelial carcinoma formed from subcutaneously injected scramble-siRNA SP cells. Bmi1 is crucial for the maintenance of stemness properties and tumorigenicity of human bladder CSC-like cells. Bmi1 may be a potential therapeutic target for the eradication of CSCs in bladder cancer.
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The kinin B1 receptor mediates alloknesis in a murine model of inflammation.
Neurosci. Lett.
PUBLISHED: 09-21-2013
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Noxious stimuli and non-noxious mechanical stimuli elicit itch (alloknesis) instead of pain on skin lesions of patients with atopic dermatitis. We previously found that bradykinin evokes an itch-related scratching response through activation of kinin B1 receptor in skin inflamed using complete Freunds adjuvant. In this study we investigated whether alloknesis is evoked in CFA-inflamed skin and the involvement of kinin receptors. In our results, alloknesis was elicited four days after CFA-inflammation. Furthermore, pretreatment with a B1 receptor antagonist or ?-opioid receptor antagonist significantly reduced alloknesis. In contrast, treatment with a B2 receptor antagonist significantly increased alloknesis. These results suggest that the alloknesis response is mediated by the activation of kinin B1 receptor but antagonized by the B2 receptor in CFA-inflamed mice.
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Comprehensive assessment of the association between DNA repair gene XRCC3 Thr241Met polymorphism and leukemia risk.
Tumour Biol.
PUBLISHED: 09-21-2013
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The XRCC3 gene has been suggested to play an important role in the pathogenesis of leukemia risk. But the findings of publications are contradictory. To derive a more precise estimation of the association, we performed a meta-analysis. The PubMed, Embase, and China National Knowledge Infrastructure (CNKI) databases were searched for case-control studies published up to August 2013. The pooled odds ratio (OR) and its corresponding 95 % confidence interval (CI) were calculated by using a fixed- or random-effect model. A total of 15 case-control studies met the inclusion criteria and were selected. The pooled OR showed that there was no statistically significant association between XRCC3 Thr241Met polymorphism and leukemia risk in overall including studies, while a risky association was observed for acute myeloid leukemia (AML) (dominant model TT/TC vs. CC: OR = 1.240, 95 % CI = 1.018-1.511, P = 0.032). The XRCC3 Thr241Met polymorphism might be associated with risk of leukemia in AML. More studies with larger sample sizes are needed to validate this result.
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[Mechanism study on anti-proliferative effects of curcumol in human hepatocarcinoma HepG2 cells].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 09-10-2013
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To investigate the anti-proliferative effects of curcumol, an herbal extract from curcuma, in human hepatocarcinoma HepG2 cells, and its possible molecular mechanism.
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The head module of Mediator directs activation of preloaded RNAPII in vivo.
Nucleic Acids Res.
PUBLISHED: 09-04-2013
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The successful synthesis of a transcript by RNA polymerase II (RNAPII) is a multistage process with distinct rate-limiting steps that can vary depending on the particular gene. A growing number of genes in a variety of organisms are regulated at steps after the recruitment of RNAPII. The best-characterized Saccharomyces cerevisiae gene regulated in this manner is CYC1. This gene has high occupancy of RNAPII under non-inducing conditions, defining it as a poised gene. Here, we find that subunits of the head module of Mediator, Med18 and Med20, and Med19 are required for activation of transcription at the CYC1 promoter in response to environmental cues. These subunits of Mediator are required at the preloaded promoter for normal levels of recruitment and activity of the general transcription factor TFIIH. Strikingly, these Mediator components are dispensable for activation by the same activator at a different gene, which lacks a preloaded polymerase in the promoter region. Based on these results and other studies, we speculate that Mediator plays an essential role in triggering an inactive polymerase at CYC1 into a productively elongating form.
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Association of XRCC3 Thr241Met polymorphisms and gliomas risk: evidence from a meta-analysis.
Asian Pac. J. Cancer Prev.
PUBLISHED: 09-03-2013
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The relationship between the X-ray repair cross-complementing group 3 (XRCC3) Thr241Met polymorphism and gliomas remains inclusive or controversial. For better understanding of the effect of XRCC3 Thr241Met polymorphism on glioma risk, a meta-analysis was performed. All eligible studies were identified through a search of PubMed, Elsevier Science Direct, Excerpta Medica Database (Embase) and Chinese Biomedical Literature Database (CBM) before May 2013. The association between the XRCC3 Thr241Met polymorphism and gliomas risk was conducted by odds ratios (ORs) and 95% confidence intervals (95% CIs). A total of nine case-control studies including 3,533 cases and 4,696 controls were eventually collected. Overall, we found that XRCC3 Thr241Met polymorphism was significantly associated with the risk of gliomas (T vs. C: OR=1.10, 95%CI=1.01-1.20, P=0.034; TT vs. CC: OR=1.30, 95%CI=1.03-1.65, P=0.027; TT vs.
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Quantitative assessment of the associations between DNA repair gene XRCC3 Thr241Met polymorphism and gastric cancer.
Tumour Biol.
PUBLISHED: 08-26-2013
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Gastric cancer is one of the most prevalent types of cancer in the world today. Recently, there is a large sample volume of published case-control studies on XRCC3 Thr241Met polymorphism with gastric cancer. This will have an important impact on published article by meta-analysis regarding XRCC3 Thr241Met polymorphism with gastric cancer. Therefore, it is necessary to update by meta-analysis to comprehensively investigate the relationship between DNA repair gene XRCC3 Thr241Met polymorphism and gastric cancer. To preciously examine the association between the XRCC3 Thr241Met polymorphism and gastric cancer, we undertook a meta-analysis of 12 case-control studies. The association between the XRCC3 Thr241Met polymorphism and gastric cancer risk was assessed by odds ratios together with their 95 % confidence intervals using a fixed-effects model or random-effects model. We found that the XRCC3 Thr241Met polymorphism was not associated with an increased gastric cancer risk. However, the XRCC3 Thr241Met polymorphism was associated with decreased gastric cancer risk in Asians. In addition, there is no evidence of association on XRCC3 Thr241Met polymorphism with response to chemotherapy and prognosis in gastric cancer. The XRCC3 Thr241Met polymorphism might influence gastric cancer risk in Asians.
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Succinic acid production from corn stalk hydrolysate in an E. coli mutant generated by atmospheric and room-temperature plasmas and metabolic evolution strategies.
J. Ind. Microbiol. Biotechnol.
PUBLISHED: 07-28-2013
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AFP111 is a spontaneous mutant of Escherichia coli with mutations in the glucose-specific phosphotransferase system, pyruvate formate lyase system, and fermentative lactate dehydrogenase system, created to reduce byproduct formation and increase succinic acid accumulation. In AFP111, conversion of xylose to succinic acid only generates 1.67 ATP per xylose, but requires 2.67 ATP for xylose metabolism. Therefore, the ATP produced is not adequate to accomplish the conversion of xylose to succinic acid in chemically defined medium. An E. coli mutant was obtained by atmospheric and room-temperature plasmas and metabolic evolution strategies, which had the ability to use xylose and improve the capacity of cell growth. The concentration of ATP in the mutant was 1.33-fold higher than that in AFP111 during xylose fermentation. In addition, under anaerobic fermentation with almost 80 % xylose from corn stalk hydrolysate, a succinic acid concentration of 21.1 g l(-1) was obtained, with a corresponding yield of 76 %.
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Genetic analysis of Tn916-like elements conferring tetracycline resistance in clinical isolates of Clostridium difficile.
Int. J. Antimicrob. Agents
PUBLISHED: 07-22-2013
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As an important clinically relevant pathogen, Clostridium difficile has a high multidrug resistance rate. Conjugative transposons play a vital role in its resistance phenotype. In the present study, 34 tetracycline-resistant clinical isolates of C. difficile were studied to detect tetracycline resistance genes and the presence of transposons. Thirty-two isolates were found to harbour Tn916-like elements carrying the tet(M) resistance gene, of which only one copy existed in the genome by Southern blot analysis. To characterise the genetic organisation of the Tn916-like elements, overlap PCR assays were performed with nine primer pairs, revealing three types of elements designated T1 to T3. The prevalent element T1 lacking PCRA (ORF23 to ORF21) and PCRB (ORF21 to ORF20) products, present in the epidemic ST37 clone, was further analysed by genome walking PCR in the left and right end sequences of the novel Tn916-like element. A gene coding for an FtsK/SpoIIIE family protein was found to replace the ORF24 to ORF21 region in Tn916. Moreover, the element could hardly conjugate between cells by filter mating experiments. These findings suggest that the dissemination of Tn916-like elements in epidemic ST37 strains in China was likely to have been conferred by clonal spread, signifying the importance of future surveillance and characterisation of conjugative transposons.
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[Functions of ANAC092 involved in regulation of anther development in Arabidopsis thaliana].
Yi Chuan
PUBLISHED: 07-16-2013
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NAC family is a class of transcription factors that have been typically found in plant with various functions. This type of genes plays a key regulatory role in secondary plant growth, cell division, senescence, especially in the hormone and signaling pathways. ANAC092 was reported to function in relation to lateral root development and senescence. The promoter and in situ hybridization analyses showed that ANAC092 was expressed temporally in the process of anther development. The gene was expressed in tapetum at stages 8-11 of anther development and reached the highest amount at stages 9-10, which was overlapped with the expression period of AMS (Aborted microspores). In this study, we constructed ANAC092 over-expression system, and identified homozygous transgenic lines. Compared with the wild type, the number of pollen grains in the transgenic line was decreased significantly, but the length of pollen grain was increased. qRT-PCR analysis showed that the expression of genes related to pollen development, e.g., SPL, EMS1, DYT1, and AMS was in-creased in the over-expression plants. Bioinformatics results showed that ANAC092 promoter sequence possessed seven AMS binding sites. All the results showed that ANAC092 is possibly located in the downstream of AMS and plays an im-portant role in the process of pollen development.
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