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Find video protocols related to scientific articles indexed in Pubmed.
Pillar[6]arene-Valved Mesoporous Silica Nanovehicles for Multiresponsive Controlled Release.
ACS Appl Mater Interfaces
PUBLISHED: 10-23-2014
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The synthesis and host-guest chemistry of pillararene (PA) derivatives are a hot research topic, and the applications of PAs in relevant research fields are essential to explore. Carboxylate-substituted pillar[6]arene (CPA[6])-valved mesoporous silica nanoparticles (MSNs) functionalized with dimethylbenzimidazolium (DMBI) and bipyridinium (BP) stalks were constructed, respectively, for multiresponsive controlled release. CPA[6] encircled the DMBI or BP stalks to develop supramolecular nanovalves for encapsulation of cargo within the MSN pores. The release of cargo was triggered by acidic pH or competitive binding for the dethreading of CPA[6] and the opening of the nanovalves; moreover, coordination chemistry is the first strategy to activate CPA nanovalves by metal chelating with the carboxylate groups of CPA for cargo release. The controlled release of the CPA[6]-valved MSN delivery systems can meet diverse requirements and has promising biological applications in targeted drug therapy.
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Highly regioselective synthesis of 3-alkenyl-oxindole ring-fused 3,3'-disubstituted oxindoles via direct gamma-substitution of Morita-Baylis-Hillman carbonates of isatins with 3-substituted oxindoles.
Org. Biomol. Chem.
PUBLISHED: 10-14-2014
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The first phase transfer-catalysed direct ?-substitution of Morita-Baylis-Hillman carbonates of isatins with 3-substituted oxindoles has been developed, which affords 3-alkenyl-oxindole ring-fused 3,3'-disubstituted oxindoles in up to 83% yield under mild reaction conditions. Furthermore, their biological activity has been preliminarily demonstrated by in vitro evaluation against human prostate cancer cells PC-3 and human leukemia cells K562, using MTT-based assays with the commercially available standard drug Cisplatin as a positive control. Gratifyingly, compounds 3aa, 3ba and 3ca exhibited comparable in vitro inhibitory activities against human prostate cancer cells (PC-3) to Cisplatin. What's more, 3ba also had a good inhibition ability against human leukemia cells K562. These results indicate that 3-alkenyl-oxindole ring-fused 3,3'-disubstituted oxindole analogs may be potential lead compounds for further biological screening.
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Psychosocial adjustments in patients with prostate cancer from pre-diagnosis to 6 months post-treatment.
Int J Nurs Pract
PUBLISHED: 10-14-2014
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We evaluated changes in psychosocial adjustment over time and its associated factors in prostate cancer patients. A total of 69 patients with prostate cancer were surveyed at pre-diagnosis, 1 month and 6 months post-treatment. The questionnaires distributed to the patients consisted of the Psychosocial Adjustment to Illness Scale and the UCLA Prostate Cancer Index. The generalized estimating equations were used to analyse the collected data. The results of adjustments to psychological distress, the domestic environment and the social environment worsened post-treatment. However, the adjustment to health-care orientation was worst at the time of pre-diagnosis and improved during post-treatment. Patients who perceived an unfavourable health status reported poor adjustment in psychological distress. Patients exhibiting poor urinary function poorly adjusted to the domestic environment. Patients with sexual dysfunction exhibited poor adjustment to the social environment. Patients with low education demonstrated poor adjustment to health-care orientation. Further studies should assess the psychosocial adjustment among prostate cancer patients and provide interventions following pre-diagnosis.
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Silencing of miRNA-148a by hypermethylation activates the integrin-mediated signaling pathway in nasopharyngeal carcinoma.
Oncotarget
PUBLISHED: 10-04-2014
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MicroRNAs (miRNAs) play a pivotal role in carcinogenesis by suppressing oncogenes or tumor suppressor genes. Various studies have identified numerous miRNAs and their diverse targets; however, the consequences of dysregulated miRNAs in nasopharyngeal carcinoma (NPC) remain unclear. For this study, we found that miR-148a is downregulated through hypermethylation in NPC biopsies and NPC cell lines compared with adjacent normal and NP cells respectively. Promoter assays demonstrated that upstream stimulatory factor 1 (USF1) is a crucial transcription factor that activates miR-148a promoter activity. EMSA assays confirmed that purified USF1 binds better toward the unmethylated than the methylated CG-containing USF1 consensus probe. The ectopic expression of miR-148a inhibits cell migration in NPC cells through the suppression of integrin-mediated signaling by targeting VAV2, WASL and ROCK1. Biochemical and functional assays provided supporting evidence that these 3 genes are the downstream targets of miR-148a in NPC cells. Furthermore, immunohistochemical staining and Western blotting analysis revealed that the 3 oncogenic targets of miR-148a were overexpressed in NPC biopsies, suggesting that the inactivation of miR-148a caused by DNA methylation promotes NPC progression. Overall, our findings revealed that miR-148a can act as tumor suppressor miRNA and serve as a biomarker as well as a therapeutic target for NPC.
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[A cadaveric study on establishing an individualized navigation template for the placement of occipital condyle screws using a three-dimensional printing technique].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 09-30-2014
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To investigate the feasibility of establishing an individualized navigation template for occipital condyle screws insertion using a fused deposition modeling based three-dimensional printing forming technique, and to evaluate the accuracy and safety of template-assisted condyle screw insertion.
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Characteristics of the transmission of autoregressive sub-patterns in financial time series.
Sci Rep
PUBLISHED: 09-05-2014
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There are many types of autoregressive patterns in financial time series, and they form a transmission process. Here, we define autoregressive patterns quantitatively through an econometrical regression model. We present a computational algorithm that sets the autoregressive patterns as nodes and transmissions between patterns as edges, and then converts the transmission process of autoregressive patterns in a time series into a network. We utilised daily Shanghai (securities) composite index time series to study the transmission characteristics of autoregressive patterns. We found statistically significant evidence that the financial market is not random and that there are similar characteristics between parts and whole time series. A few types of autoregressive sub-patterns and transmission patterns drive the oscillations of the financial market. A clustering effect on fluctuations appears in the transmission process, and certain non-major autoregressive sub-patterns have high media capabilities in the financial time series. Different stock indexes exhibit similar characteristics in the transmission of fluctuation information. This work not only proposes a distinctive perspective for analysing financial time series but also provides important information for investors.
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Fast-track surgery versus traditional perioperative care in laparoscopic colorectal cancer surgery: a meta-analysis.
BMC Cancer
PUBLISHED: 08-23-2014
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Both laparoscopic and fast-track surgery (FTS) have shown some advantages in colorectal surgery. However, the effectiveness of using both methods together is unclear. We performed this meta-analysis to compare the effects of FTS with those of traditional perioperative care in laparoscopic colorectal cancer surgery.
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Synergetic gating of metal-latching ligands and metal-chelating proteins for mesoporous silica nanovehicles to enhance delivery efficiency.
ACS Appl Mater Interfaces
PUBLISHED: 08-19-2014
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Stimuli-responsive drug delivery systems are highly desirable for improved therapeutic efficacy and minimized adverse effects of drugs. Mesoporous silica nanoparticles (MSNs) functionalized with pentadentate ligands, N-(3-trimethoxysilylpropyl)ethylenediamine triacetate (TSP-DATA), in the presence of metal ions with and without myoglobin (Mb)-containing surface-accessible histidine residues, were constructed for pH-triggered controlled release. The DATA ligands immobilized on the MSN pore outlets could encapsulate cargo within the pores by metal latching across pore openings, and release efficiency increased with the increase of surface density of the DATA ligands. The release efficiencies for the metal-chelating protein nanogates, through multiple-site binding of Mb with the metal-chelating ligands, were higher than those for the metal-latching ligand nanogates but were almost independent of surface density of the ligands investigated. Both the metal-latching ligands and the metal-chelating proteins played a synergetic role in gating MSNs for high-loading drug delivery and stimuli-responsive controlled release. The constructed Mb-Cu(2+)-gated MSN delivery system has promising applications in targeted drug therapy of tumors.
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IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection.
Cell. Microbiol.
PUBLISHED: 08-11-2014
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Interferon-inducible transmembrane proteins 1, 2 and 3 (IFITM1, IFITM2 and IFITM3) have recently been identified as potent antiviral effectors that function to suppress the entry of a broad range of enveloped viruses and modulate cellular tropism independent of viral receptor expression. However, the antiviral effect and mechanisms of IFITMs in response to viral infections remain incompletely understood and characterized. In this work, we focused our investigation on the function of the extracellular IFITM3 protein. In cell models of DENV-2 infection, we found that IFITM3 contributed to both the baseline and interferon-induced inhibition of DENV entry. Most importantly, our study for the first time demonstrated the presence of IFITM-containing exosome in the extracellular environment, and identified an ability of cellular exosome to intercellularly deliver IFITM3 and thus transmit its antiviral effect from infected to non-infected cells. Thus, our findings provide new insights in the basic mechanisms underlying the actions of IFITM3, which might lead to future development of exosome-mediated anti-viral strategies using IFITM3 as a therapeutic agent. Conceivably, variations in the basal and inducible levels of IFITMs, as well as in intracellular and extracellular levels of IFITMs, might predict the severity of dengue virus infections among individuals or across species.
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Transmission of linear regression patterns between time series: from relationship in time series to complex networks.
Phys Rev E Stat Nonlin Soft Matter Phys
PUBLISHED: 07-31-2014
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The linear regression parameters between two time series can be different under different lengths of observation period. If we study the whole period by the sliding window of a short period, the change of the linear regression parameters is a process of dynamic transmission over time. We tackle fundamental research that presents a simple and efficient computational scheme: a linear regression patterns transmission algorithm, which transforms linear regression patterns into directed and weighted networks. The linear regression patterns (nodes) are defined by the combination of intervals of the linear regression parameters and the results of the significance testing under different sizes of the sliding window. The transmissions between adjacent patterns are defined as edges, and the weights of the edges are the frequency of the transmissions. The major patterns, the distance, and the medium in the process of the transmission can be captured. The statistical results of weighted out-degree and betweenness centrality are mapped on timelines, which shows the features of the distribution of the results. Many measurements in different areas that involve two related time series variables could take advantage of this algorithm to characterize the dynamic relationships between the time series from a new perspective.
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High frequency of circulating follicular helper T cells in patients with bronchial asthma.
Clin. Lab.
PUBLISHED: 07-15-2014
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Bronchial asthma is a chronic airway inflammatory disease primarily characterized by an abnormality in the IgE pathway. Follicular helper T (Tfh) cells are the specialized subset indispensible for providing cognate help to B cells during formation of germinal centers. The more recent work presents clear evidence that human blood CD4+ CXCR5+ T cells, circulating Tfh (cTfh) cells, are counterparts of Tfh cells in germinal centers.
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miR?96 functions as a tumor suppressor gene by targeting NUAK1 in pancreatic cancer.
Int. J. Mol. Med.
PUBLISHED: 06-13-2014
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microRNA?96 (miR?96) is known to be downregulated in pancreatic cancer. The overexpression of miR?96 in MIA PaCa?2 pancreatic cancer cells has been shown to inhibit cell proliferation, migration and invasion; however, the mechanisms involved have not yet been fully elucidated. Novel (nua) kinase family 1 (NUAK1) functions as an oncogene in non?small cell lung cancer (NSCLC), melanoma, glioma, breast cancer, hepatocellular carcinoma and pancreatic cancer. In this study, firstly, we demonstrate that NUAK1 expression is specifically upregulated in pancreatic cancer and that it promotes the proliferation, migration and invasion of MIA PaCa?2 pancreatic cancer cells. Secondly, we performed an analysis of potential microRNA (miRNA) target sites using three commonly used prediction algorithms: miRanda, TargetScan and PicTar. All three algorithms predicted that miR?96 targets the 3' untranslated region (3' UTR) of NUAK1. Further experiments confirmed this prediction, namely that miR?96 suppresses the expression of NUAK1 by targeting its 3' UTR. Finally, we demonstrate that the introduction of NUAK1 cDNA lacking predicted sites of the 3' UTR abrogates miR?96 cellular function.
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Biphasic ER-?36-mediated estrogen signaling regulates growth of gastric cancer cells.
Int. J. Oncol.
PUBLISHED: 06-10-2014
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To examine the expression patterns of ER-?36 and Cyclin D1 in human gastric cancer tissues and to investigate the effects of ER-?36-mediated estrogen signaling on the growth of gastric cancer cells, 117 samples of formalin-fixed and paraffin-embedded gastric cancer tumor tissues and 40 fresh gastric cancer tumor tissues were analyzed with immunohistochemistry assay and western blot analysis. ER-?36 expression was well correlated with gender (male:female ratio 2.88:1, P=0.01), invasion to serosa (P=0.01) as well as Cyclin D1 expression (P<0.01). The effects of different concentrations of estrogen on the growth of different gastric cancer cells and normal gastric cells as well as gastric cancer SGC7901 cells with different levels of ER-?36 expression were examined. SGC7901 cells with high levels of ER-?36 expression exhibited estrogen hypersensitivity, high growth rate and high levels of Cyclin D1 expression while SGC7901 cells with knocked-down levels of ER-?36 expression were insensitive to estrogen stimulation, grew slowly and expressed less Cyclin D1. Our results indicate that ER-?36 mediates biphasic estrogen signaling in the growth of gastric cancer cells.
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Prenatal management and outcomes in mirror syndrome associated with twin-twin transfusion syndrome.
Prenat. Diagn.
PUBLISHED: 05-15-2014
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The aim of this article is to investigate the prevalence, clinical presentation, prenatal management, and prognosis of mirror syndrome associated with twin-twin transfusion syndrome (TTTS) treated by amnioreduction or selective fetocide.
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[Effect of Weifuchun on inhibiting inflammation of Helicobacter pylori-infected GES-1 cells and NF-kappaB signaling pathway].
Zhongguo Zhong Xi Yi Jie He Za Zhi
PUBLISHED: 05-13-2014
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To study the effect of Weifuchun on inflammation of Helicobacter pylori (Hp)-infected gastric epithelial cells (GES-1) and its correlation with NF-kappaB signaling pathway.
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miR-219 inhibits the proliferation, migration and invasion of medulloblastoma cells by targeting CD164.
Int. J. Mol. Med.
PUBLISHED: 04-01-2014
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It is known that microRNA-219 (miR-219) expression is downregulated in medulloblastoma. In the present study, we investigated the expression, targets and functional effects of miR-219 in D283-MED medulloblastoma cells. We first demonstrated that miR-219 not only inhibits proliferation, but also suppresses the invasion and migration of D283-MED cells. Moreover, the knockdown of miR-219 promoted the proliferation, migration and invasion of the D283-MED cells. Secondly, we predicted that miR-219 targets the 3' untranslated region (3'UTR) of CD164 and orthodenticle homeobox 2 (OTX2) and then confirmed that it significantly downregulated the protein expression of CD164 and OTX2 in D283-MED cells. Finally, we demonstrated that the proliferation, invasion and migration of D283-MED cells were promoted by theectopic expression of CD164. These results indicate that miR-219 suppresses the proliferation, migration and invasion of medulloblastoma cells by targeting CD164. The results also suggest that miR-219 may serve as a potential therapeutic agent for medulloblastoma.
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Enhanced p62 expression triggers concomitant autophagy and apoptosis in a rat chronic spinal cord compression model.
Mol Med Rep
PUBLISHED: 03-10-2014
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Chronic spinal cord compression is the result of mechanical pressure on the spinal cord, which in contrast to traumatic spinal cord injury, leads to slowly progressing nerve degeneration. These two types of spinal cord injuries may trigger similar mechanisms, including motoric nerve cell apoptosis and autophagy, however, depending on differences in the underlying injury severity, nerve reactions may predominantly involve the conservation of function or the initiation of functions for the removal of irreversibly damaged cells. p62 is a multidomain adapter protein, which is involved in apoptosis and cell survival as well as autophagy, and is a common component of protein aggregations in neurodegenerative diseases. In the present study, a rat chronic spinal cord compression model was used, in which the spinal cord was progressively compressed for six weeks and then constantly compressed for another 10 weeks. As a result Basso, Beattie and Bresnahan locomotor scaling revealed a gradual score decrease until the 6th week followed by constant recovery until the 16th week after spinal cord compression was initiated. During the first eight weeks of the experiment, p62 and nuclear factor-?B (NF-?B) were increasingly expressed up to a constant plateau at 12-16 weeks, whereas caspase 3 exhibited a marginally enhanced expression at 8 weeks, however, reached a constant maximum peak 12-16 weeks after the beginning of spinal cord compression. It was hypothesized that, in the initial phase of spinal cord compression, enhanced p62 expression triggered NF-?B activity, directing the cell responses mainly to cell survival and autophagy, whereas following eight weeks of spinal cord compression, caspase 3 was additionally activated indicating cumulative elimination of irreversibly damaged nerve cells with highly activated autophagy.
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Three epilepsy-associated GABRG2 missense mutations at the ?+/?- interface disrupt GABAA receptor assembly and trafficking by similar mechanisms but to different extents.
Neurobiol. Dis.
PUBLISHED: 03-03-2014
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We compared the effects of three missense mutations in the GABAA receptor ?2 subunit on GABAA receptor assembly, trafficking and function in HEK293T cells cotransfected with ?1, ?2, and wildtype or mutant ?2 subunits. The mutations R82Q and P83S were identified in families with genetic epilepsy with febrile seizures plus (GEFS+), and N79S was found in a single patient with generalized tonic-clonic seizures (GTCS). Although all three mutations were located in an N-terminal loop that contributes to the ?+/?- subunit-subunit interface, we found that each mutation impaired GABAA receptor assembly to a different extent. The ?2(R82Q) and ?2(P83S) subunits had reduced ?1?2?2 receptor surface expression due to impaired assembly into pentamers, endoplasmic reticulum (ER) retention and degradation. In contrast, ?2(N79S) subunits were efficiently assembled into GABAA receptors with only minimally altered receptor trafficking, suggesting that N79S was a rare or susceptibility variant rather than an epilepsy mutation. Increased structural variability at assembly motifs was predicted by R82Q and P83S, but not N79S, substitution, suggesting that R82Q and P83S substitutions were less tolerated. Membrane proteins with missense mutations that impair folding and assembly often can be "rescued" by decreased temperatures. We coexpressed wildtype or mutant ?2 subunits with ?1 and ?2 subunits and found increased surface and total levels of both wildtype and mutant ?2 subunits after decreasing the incubation temperature to 30°C for 24h, suggesting that lower temperatures increased GABAA receptor stability. Thus epilepsy-associated mutations N79S, R82Q and P83S disrupted GABAA receptor assembly to different extents, an effect that could be potentially rescued by facilitating protein folding and assembly.
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Audio-visual biofeedback does not improve the reliability of target delineation using maximum intensity projection in 4-dimensional computed tomography radiation therapy planning.
Int. J. Radiat. Oncol. Biol. Phys.
PUBLISHED: 02-12-2014
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To investigate whether coaching patients' breathing would improve the match between ITVMIP (internal target volume generated by contouring in the maximum intensity projection scan) and ITV10 (generated by combining the gross tumor volumes contoured in 10 phases of a 4-dimensional CT [4DCT] scan).
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Synthesis, structure, luminescence and catalytic properties of cadmium(ii) coordination polymers with 9H-carbazole-2,7-dicarboxylic acid.
Dalton Trans
PUBLISHED: 01-16-2014
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Two Cd(ii) metal-organic frameworks were synthesized from the NH-functionalized dicarboxylate ligand 9H-carbazole-2,7-dicarboxylic acid (2,7-H2CDC). Compound , [Cd4(CDC)4(DMF)4]·4DMF·4H2O, displays 2D square grid networks based on novel tetranuclear [Cd4(COO)8] secondary building units (SBUs) and pairwise CDC(2-) linkers. Compound , (H3O)2[Cd3(2,7-CDC)4]·3DMF·4H2O, is also based on 4-connected SBUs and pairwise CDC linkers, but the unusual trinuclear [Cd3(COO)8] SBUs lead to 2-fold interpenetrated 3D diamond-type frameworks with guest accessible voids. Both compounds display strong blue fluorescence in the solid state, and compound shows high catalytic activity for Knoevenagel condensation.
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Understanding Strategy of Nitrate and Urea Assimilation in a Chinese Strain of Aureococcus anophagefferens through RNA-Seq Analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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Aureococcus anophagefferens is a harmful alga that dominates plankton communities during brown tides in North America, Africa, and Asia. Here, RNA-seq technology was used to profile the transcriptome of a Chinese strain of A. anophagefferens that was grown on urea, nitrate, and a mixture of urea and nitrate, and that was under N-replete, limited and recovery conditions to understand the molecular mechanisms that underlie nitrate and urea utilization. The number of differentially expressed genes between urea-grown and mixture N-grown cells were much less than those between urea-grown and nitrate-grown cells. Compared with nitrate-grown cells, mixture N-grown cells contained much lower levels of transcripts encoding proteins that are involved in nitrate transport and assimilation. Together with profiles of nutrient changes in media, these results suggest that A. anophagefferens primarily feeds on urea instead of nitrate when urea and nitrate co-exist. Furthermore, we noted that transcripts upregulated by nitrate and N-limitation included those encoding proteins involved in amino acid and nucleotide transport, degradation of amides and cyanates, and nitrate assimilation pathway. The data suggest that A. anophagefferens possesses an ability to utilize a variety of dissolved organic nitrogen. Moreover, transcripts for synthesis of proteins, glutamate-derived amino acids, spermines and sterols were upregulated by urea. Transcripts encoding key enzymes that are involved in the ornithine-urea and TCA cycles were differentially regulated by urea and nitrogen concentration, which suggests that the OUC may be linked to the TCA cycle and involved in reallocation of intracellular carbon and nitrogen. These genes regulated by urea may be crucial for the rapid proliferation of A. anophagefferens when urea is provided as the N source.
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TCR??(+)CD4(-)CD8(-) T cells suppress the CD8(+) T-cell response to hepatitis B virus peptides, and are associated with viral control in chronic hepatitis B.
PLoS ONE
PUBLISHED: 01-01-2014
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The immune mechanisms underlying failure to achieve hepatitis B e antigen (HBeAg) seroconversion associated with viral control in chronic hepatitis B (CHB) remain unclear. Here we investigated the role of CD4(-)CD8(-) T (double-negative T; DNT) cells including TCR??(+) DNT (?? DNT) and TCR??(+) DNT (?? DNT) cells. Frequencies of circulating DNT cell subsets were measured by flow cytometry in a retrospective cohort of 51 telbivudine-treated HBeAg-positive CHB patients, 25 immune tolerant carriers (IT), 33 inactive carriers (IC), and 37 healthy controls (HC). We found that ?? DNT cell frequencies did not significantly change during treatment, being lower at baseline (P?=?0.019) in patients with HBeAg seroconversion after 52 weeks of antiviral therapy (n?=?20) than in those without (n?=?31), and higher in the total CHB and IT than IC and HC groups (P<0.001). ?? DNT cell frequencies were similar for all groups. In vitro, ?? DNT cells suppressed HBV core peptide-stimulated interferon-? and tumor necrosis factor-? production in TCR??(+)CD8(+) T cells, which may require cell-cell contact, and could be partially reversed by anti-NKG2A. These findings suggest that ?? DNT cells limit CD8(+) T cell response to HBV, and may impede HBeAg seroconversion in CHB.
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[Perinatal outcome of monochorionic diamniotic twins with selective intrauterine growth restriction].
Zhonghua Fu Chan Ke Za Zhi
PUBLISHED: 10-10-2013
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To evaluate the perinatal outcome of three types of monochorionic diamniotic (MCDA) twins with selective intrauterine growth restriction (sIUGR).
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[Application of joint reconstruction with autogenous coronoid process graft to treat temporomandibular joint ankylosis].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 10-09-2013
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To evaluate the clinical effect of joint reconstruction by using autogenous coronoid process graft to treat temporomandibular joint(TMJ) ankylosis.
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Preoperative Colonic Stents Versus Emergency Surgery for Acute Left-Sided Malignant Colonic Obstruction: A Meta-analysis.
J. Gastrointest. Surg.
PUBLISHED: 06-22-2013
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The purpose of this paper is to evaluate the efficacy and safety of colonic stenting as a bridge to surgery versus emergency surgery for acute left-sided malignant colonic obstruction.
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Creating protein-imprinted self-assembled monolayers with multiple binding sites and biocompatible imprinted cavities.
J. Am. Chem. Soc.
PUBLISHED: 06-17-2013
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Imprinted monolayers have several advantages over bulk imprinted polymers such as excellent mass transfer of molecules into and out of imprinted sites and transduction of binding signals detected in real time. Protein-imprinted self-assembled monolayers (SAMs) were created with multiple binding sites and biocompatible imprinted cavities from functional thiols and novel disulfide compounds containing an oligoethylene glycol (OEG) terminal moiety and two amide groups incorporated in the chain (DHAP) in a biologically benign solution. DHAP played an important role in the formation of multiple binding sites and biocompatible cavities in addition to resisting nonspecific protein binding. The created protein-imprinted SAMs exhibited the excellent ability of specific binding of target proteins determined by multiple binding sites and imprinted cavities. The strategy generates tailor-made monolayer surfaces with specific protein binding and opens the possibility of controlled assembly of intellectual biomaterials and preparation of biosensors.
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Taishan Pinus massoniana pollen polysaccharides promote immune responses of recombinant Bordetella avium ompA in BALB/c mice.
Int. Immunopharmacol.
PUBLISHED: 06-08-2013
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To study the effects of Taishan Pinus massoniana pollen polysaccharides (TPPPS) on Bordetella avium outer membrane protein A (ompA) recombinant protein vaccine, ompA was expressed, confirmed by Western blotting and mixed with TPPPS. Female BALB/c mice were randomly divided into six groups (I-VI). Groups I, II, and III were treated with TPPPS-ompA at doses of 200, 400, and 800 mg/ml, respectively. Groups IV, V, and VI were treated with Freunds adjuvant-ompA, pure ompA, and physiological saline, respectively. On days 3, 7, 14, 21, 28, 35, 42, and 49 after the first vaccination, antibody titers, interleukin-2 (IL-2) levels, peripheral blood CD4+ and CD8+ levels, and T lymphocyte proliferation rates in peripheral blood, as well as secreting-type immunoglobulin A (SIgA) levels in the duodenum, were measured. The antibody titers against ompA, IL-2, T lymphocyte proliferation rate, CD4+, and CD8+ in Group II were significantly (P<0.05) higher than those in other groups. However, little difference in SIgA content was observed among Groups I, II, and IV. These results indicated that TPPPS strengthened humoral and cellular immune response against recombinant ompA vaccine and 400 mg/ml TPPPS showed significance (P<0.05) compared with Freunds adjuvant. Therefore, TPPPS can be developed into an adjuvant for recombinant protein vaccines or plant-derived medicine for animal husbandry.
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Interleukin-27 as a potential therapeutic target for rheumatoid arthritis: has the time come?
Clin. Rheumatol.
PUBLISHED: 05-22-2013
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Interleukin (IL)-27 is a novel member of the IL-6/IL-12 family of cytokines with a broad range of pro- and anti-inflammatory properties. Recently, accumulating evidence has shown that IL-27 can play either a pathogenic or a protective role in animal models of inflammatory arthritis, depending upon the model and underlying pathogenic mechanisms. As to human system, elevated expression of IL-27 has clearly been detected in the synovial membranes and fluid from patients with rheumatoid arthritis (RA). Moreover, stimulation of IL-27 receptor with IL-27 of fibroblast-like synoviocytes from RA had a suppressive effect on the production of proinflammatory cytokines in vitro. All these findings suggest that IL-27 may have promise as a potential therapeutic target for RA. In this review, we will discuss the biological features of IL-27 and summarize recent advances on both pathogenic and protective roles of IL-27 in RA.
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Levosimendan versus dobutamine in critically ill patients: a meta-analysis of randomized controlled trials.
J Zhejiang Univ Sci B
PUBLISHED: 05-07-2013
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To evaluate the clinical efficacy of levosimendan versus dobutamine in critically ill patients requiring inotropic support.
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Effects of radix curcumae-derived diterpenoid C on Helicobacter pylori-induced inflammation and nuclear factor kappa B signal pathways.
World J. Gastroenterol.
PUBLISHED: 04-20-2013
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To study effect of diterpenoid C extracted from radix curcumae on Helicobacter pylori (H. pylori)-infected inflammation, intestinal metaplasia, and nuclear factor kappa B (NF-?B) signaling pathway in vitro.
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SERPINA3K induces apoptosis in human colorectal cancer cells via activating the Fas/FasL/caspase-8 signaling pathway.
FEBS J.
PUBLISHED: 04-19-2013
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SERPINA3K, also known as kallikrein-binding protein (KBP), is a serine proteinase inhibitor with anti-inflammatory and anti-angiogenic activities. Our previous studies showed that SERPINA3K inhibited proliferation in a dose-dependent manner and induced apoptosis of endothelial cells but had no influence on SGC-7901 gastric carcinoma cells or HepG2 hepatocarcinoma cells. However, it is unknown whether SERPINA3K has a direct impact on other carcinoma cells and which mechanisms are involved. In this study, we report for the first time that SERPINA3K not only decreased cell viability but also induced apoptosis in the colorectal carcinoma cell lines SW480 and HT-29. SERPINA3K-induced apoptosis of SW480 and HT-29 was rescued by interference with Fas ligand (FasL) small hairpin RNA. Moreover, SERPINA3K increased the expression of FasL and activated caspase-8. Peroxisome proliferator-activated receptor ? (PPAR?), a transcription factor of FasL, was also upregulated by SERPINA3K in a dose-dependent manner. The upregulation effect of FasL induced by SERPINA3K was reversed after interference with PPAR? small interfering RNA. These results demonstrated that SERPINA3K-induced SW480 and HT-29 cell apoptosis was mediated by the PPAR?/Fas/FasL signaling pathway. Therefore, our study provides additional insight into the direct anti-tumor function by inducing tumor cell apoptosis of SERPINA3K in colorectal tumors.
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Circulating chemokine (C-X-C Motif) receptor 5(+) CD4(+) T cells benefit hepatitis B e antigen seroconversion through IL-21 in patients with chronic hepatitis B virus infection.
Hepatology
PUBLISHED: 04-17-2013
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Given the clinical significance of hepatitis B e antigen (HBeAg) seroconversion in chronic hepatitis B virus (HBV) infection, it is critical to elucidate the mechanisms regulating this process. In the present study, we found that the frequency of circulating chemokine (C-X-C motif) receptor 5 (CXCR5)(+) CD4(+) T cells was higher in patients who had achieved HBeAg seroconversion in both cross-sectional (P?
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Enhanced resistance to stripe rust disease in transgenic wheat expressing the rice chitinase gene RC24.
Transgenic Res.
PUBLISHED: 03-15-2013
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Stripe rust is a devastating fungal disease of wheat worldwide which is primarily caused by Puccinia striiformis f. sp tritici. Transgenic wheat (Triticum aestivum L.) expressing rice class chitinase gene RC24 were developed by particle bombardment of immature embryos and tested for resistance to Puccinia striiformis f.sp tritici. under greenhouse and field conditions. Putative transformants were selected on kanamycin-containing media. Polymease chain reaction indicated that RC24 was transferred into 17 transformants obtained from bombardment of 1,684 immature embryos. Integration of RC24 was confirmed by Southern blot with a RC24-labeled probe and expression of RC24 was verified by RT-PCR. Nine transgenic T1 lines exhibited enhanced resistance to stripe rust infection with lines XN8 and BF4 showing the highest level of resistance. Southern blot hybridization confirmed the stable inheritance of RC24 in transgenic T1 plants. Resistance to stripe rust in transgenic T2 and T3 XN8 and BF4 plants was confirmed over two consecutive years in the field. Increased yield (27-36 %) was recorded for transgenic T2 and T3 XN8 and BF4 plants compared to controls. These results suggest that rice class I chitinase RC24 can be used to engineer stripe rust resistance in wheat.
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Noninvasive prenatal detection for pathogenic CNVs: the application in ?-thalassemia.
PLoS ONE
PUBLISHED: 01-01-2013
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The discovery of cell free fetal DNA (cff-DNA) in maternal plasma has brought new insight for noninvasive prenatal diagnosis. Combining with the rapidly developed massively parallel sequencing technology, noninvasive prenatal detection of chromosome aneuploidy and single base variation has been successfully validated. However, few studies discussed the possibility of noninvasive pathogenic CNVs detection.
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RGD peptide-mediated chitosan-based polymeric micelles targeting delivery for integrin-overexpressing tumor cells.
Int J Nanomedicine
PUBLISHED: 12-21-2011
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Solid tumors need new blood vessels to feed and nourish them as well as to allow tumor cells to escape into the circulation and lodge in other organs, which is termed "angiogenesis." Some tumor cells within solid tumors can overexpress integrins ?(v)?(3) and ?(v)?(5), which can specifically recognize the peptide motif Arg-Gly-Asp (RGD). Thus, the targeting of RGD-modified micelles to tumor vasculature is a promising strategy for tumor-targeting treatment.
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[The correlation between the level of serum VEGF and bFGF with metastasis and prognosis of osteosarcoma].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
PUBLISHED: 12-05-2011
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To investigate the correlation between the level of serum VEGF and bFGF with metastasis and re- currence of osteosarcoma.
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Restored circulating invariant NKT cells are associated with viral control in patients with chronic hepatitis B.
PLoS ONE
PUBLISHED: 09-20-2011
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Invariant NKT (iNKT) cells are involved in the pathogenesis of various infectious diseases. However, their role in hepatitis B virus (HBV) infection is not fully understood, especially in human species. In this study, 35 chronic hepatitis B (CHB) patients, 25 inactive carriers (IC) and 36 healthy controls (HC) were enrolled and the proportions of circulating iNKT cells in fresh isolated peripheral blood mononuclear cells (PBMC) were detected by flow cytometry. A longitudinal analysis was also conducted in 19 CHB patients who received antiviral therapy with telbivudine. Thereafter, the immune functions of iNKT cells were evaluated by cytokine secretion and a two-chamber technique. The median frequency of circulating iNKT cells in CHB patients (0.13%) was lower than that in HC (0.24%, P?=?0.01) and IC (0.19%, P?=?0.02), and increased significantly during antiviral therapy with telbivudine (P?=?0.0176). The expressions of CC chemokine receptor 5 (CCR5) and CCR6 were dramatically higher on iNKT cells (82.83%±9.87%, 67.67%±16.83% respectively) than on conventional T cells (30.5%±5.65%, 14.02%±5.92%, both P<0.001) in CHB patients. Furthermore, iNKT cells could migrate toward the CC chemokine ligand 5. Patients with a high ratio (?1.0) of CD4-/CD4+ iNKT cells at baseline had a higher rate (58.33%) of HBeAg seroconversion than those with a low ratio (<1.0, 0%, P?=?0.0174). In conclusion, there is a low frequency of peripheral iNKT cells in CHB patients, which increases to normal levels with viral control. The ratio of CD4-/CD4+ iNKT cells at baseline may be a useful predictor for HBeAg seroconversion in CHB patients on telbivudine therapy.
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A micro GC detector array based on chemiresistors employing various surface functionalized monolayer-protected gold nanoparticles.
Talanta
PUBLISHED: 08-17-2011
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Aspects of the design, fabrication, and characterization of a chemiresistor type of microdetector for use in conjunction with gas chromatograph are described. The detector was manufactured on silicon chips using microelectromechanical systems (MEMS) technology. Detection was based on measuring changes in resistance across a film comprised of monolayer-protected gold nanoclusters (MPCs). When chromatographic separated molecules entered the detector cell, the MPC film absorbed vapor and undergoes swelling, then the resistance changes accordingly. Thiolates were used as ligand shells to encapsulate the nano-gold core and to manipulate the selectivity of the detector array. The dimensions of the ?-detector array were 14(L)×3.9(W)×1.2(H)mm. Mixtures of eight volatile organic compounds with different functional groups and volatility were tested to characterize the selectivity of the ?-detector array. The detector responses were rapid, reversible, and linear for all of the tested compounds. The detection limits ranged from 2 to 111ng, and were related to both the compound volatility and the selectivity of the surface ligands on the gold nanoparticles. Design and operation parameters such as flow rate, detector temperature, and width of the micro-fluidic channel were investigated. Reduction of the detector temperature resulted in improved sensitivity due to increased absorption. When a wider flow channel was used, the signal-to-noise ratio was improved due to the larger sensing area. The extremely low power consumption and small size makes this ?-detector array potentially useful for the development of integrated ?-GC.
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tert-Butyl N-(4-hy-droxy-benz-yl)-N-[4-(prop-2-yn-yloxy)benz-yl]carbamate.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 08-09-2011
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In the crystal structure of the title compound, C(22)H(25)NO(4), inter-molecular O-H?O hydrogen bonds involving the hy-droxy group of the 4-(amimometh-yl)phenol fragment and the C=O group connect the mol-ecules into infinite chains along the c axis. Two C atoms of the propyne group are disordered over two sites with occupancy factors of 0.53?(2) and 0.47?(2).
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[A meta analysis of tumor necrosis factor alpha blockers therapy for ulcerative colitis].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 07-26-2011
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To pool the data of studies and evaluate the efficacy and safety of TNF? blocking agents in the treatment of ulcerative colitis (UC).
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[Preliminary clinical study on the posterior lumbar minimally invasive surgery assisted by 3D-Viewer system under a direct vision].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 07-16-2011
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To study the methods and preliminary clinical efficacy of posterior lumbar minimally invasive surgery assisted by 3D-Viewer system under a direct vision and provide rationales for further clinical applications.
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High serum IL-21 levels after 12 weeks of antiviral therapy predict HBeAg seroconversion in chronic hepatitis B.
J. Hepatol.
PUBLISHED: 07-14-2011
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Interleukin-21 (IL-21) stimulates T cell and B cell responses and plays a role in control of chronic viral infections. The role of IL-21 in chronic hepatitis B virus (HBV) infection is not understood.
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Establishment of persistent infection with foot-and-mouth disease virus in BHK-21 cells.
Virol. J.
PUBLISHED: 04-14-2011
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Foot-and-mouth disease virus (FMDV) is able to cause persistent infection in ruminants besides acute infection and disease. Since the mechanisms of viral persistence and the determining factors are still unknown, in vitro systems help explore and reveal mechanisms of persistence in vivo by providing useful models for the study of RNA genome mutations and evolution. Ammonium chloride, a lysosomotropic agent that raises intralysosomal pH, reduces the yield of FMDV during infection of BHK-21 cells.
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The antagonistic effect between STAT1 and Survivin and its clinical significance in gastric cancer.
Oncol Lett
PUBLISHED: 03-10-2011
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In previous studies, we observed that STAT1 and Survivin correlated negatively with gastric cancer tissues, and that the functions of the IFN-?-STAT1 pathway and Survivin in gastric cancer are the same as those reported for other types of cancer. In this study, the SGC7901 gastric cancer cell line and 83 gastric cancer specimens were used to confirm the relationship between STAT1 and Survivin, as well as the clinical significance of this relationship in gastric cancer. IFN-? and STAT1 and Survivin antisense oligonucleotides (ASONs) were used to knock down the expression in SGC7901 cells. The protein expression of STAT1 and Survivin was tested by immunocytochemical and image analysis methods. A gastric cancer tissue microarray was prepared and tested by immunohistochemical methods. Data were analyzed by the Spearmans rank correlation analysis, the ?(2) test and Coxs multivariate regression analysis. Upon knockdown of IFN-?, STAT1 and Survivin expression by ASON in the SGC7901 cell line, an antagonistic effect was observed between STAT1 and Survivin. In gastric cancer tissues, STAT1 showed a negative correlation with depth of invasion (p<0.05) in gastric cancer tissues exhibiting a negative Survivin protein expression. Furthermore, in tissues exhibiting a negative STAT1 protein expression, Survivin correlated negatively with N stage (p<0.05). Pathological and molecular markers were used to conduct Coxs multivariate regression analysis, and depth of invasion and N stage were found to be prognostic factors (p<0.05). On the other hand, in tissues exhibiting a negative Survivin protein expression, Coxs multivariate regression analysis revealed that the differentiation type and STAT1 protein expression were prognostic factors (p<0.05). There is an antagonistic effect between STAT1 and Survivin in gastric cancer, and this antagonistic effect is of clinical significance in gastric cancer.
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A meta-analysis of the therapeutic effects of tumor necrosis factor-? blockers on ulcerative colitis.
Eur. J. Clin. Pharmacol.
PUBLISHED: 02-18-2011
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To evaluate the therapeutic effects of TNF-? blockers on ulcerative colitis (UC) and their safety.
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A quasi-experimental investigation into the efficacy of a suicide education programme for second-year student nurses in Taiwan.
J Clin Nurs
PUBLISHED: 02-16-2011
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This study was designed to investigate the learning outcomes of a suicide education programme for second-year student nurses in Taiwan.
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The HyPRP gene EARLI1 has an auxiliary role for germinability and early seedling development under low temperature and salt stress conditions in Arabidopsis thaliana.
Planta
PUBLISHED: 01-09-2011
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The effect of the hybrid proline-rich protein (HyPRP) gene EARLI1 on the rate of germination (germinability) of Arabidopsis seeds and seedling growth under low temperature and salt stress conditions was investigated. EARLI1 was induced during germination in embryonic tissues, and was strongly expressed in certain parts of young seedlings. Comparisons of control, overexpressing (OX), and knockout (KO) lines indicated that higher than wild type levels of EARLI1 improved germinability, root elongation, and reduction of sodium accumulation in leaves under salt stress, as well as germinability under low-temperature stress. Abscisic acid (ABA) contents were relatively low after prolonged salt stress, suggesting that EARLI1 has an ABA-independent effect on germinability under these conditions. Overexpression of EARLI1 during germination enhanced the sensitivity of seeds to exogenously applied ABA, suggesting that EARLI1 has an ABA-dependent negative effect on seed germinability under high ABA stress conditions. Well-known stress response marker genes such as COR15a, KIN1, P5SC1, and RD29 were unaffected whereas P5SC2, RD22, or RAB18 were only slightly affected in OX and KO plants. The pleiotropic effects of EARLI1 during stress and an absence of strong regulatory effects on stress marker genes suggest that this HyPRP gene has an auxiliary role for various stress protection responses in Arabidopsis.
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General and specific perceptual learning in radial speed discrimination.
J Vis
PUBLISHED: 01-01-2011
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The specificity of learning in speed discrimination was examined in three psychophysical experiments. In Experiment 1, half of the observers trained with inward motion direction on a speed discrimination task, whereas the other half trained on the same task but with outward motion direction. The results indicated that significant training-based improvement transferred from a trained radial direction to an untrained radial direction. Experiment 2 confirmed this transfer by showing that complete transfer was obtained even when stimuli moving in an untrained radial direction were used in the transfer task. In Experiment 3, observers were trained at a viewing distance of 114 cm. The results showed that learning transferred partly to the viewing distances of 57 cm and 228 cm. In summary, the present transfer results indicate that reliable generalizations can be obtained in perceptual learning of radial speed discrimination.
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Complementarity-determining region 3 size spectratypes of T cell receptor beta chains in CD8+ T cells following antiviral treatment of chronic hepatitis B.
Antimicrob. Agents Chemother.
PUBLISHED: 11-22-2010
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An increased CD8(+) T cell response to hepatitis B virus (HBV) peptides occurs between 12 and 24 weeks after starting antiviral therapy for chronic hepatitis B. It is not known whether these cells have antiviral function. The aim of this study was to determine whether clonal expansions of CD8(+) T cells at these time points predict the virological response to therapy. Peripheral blood CD8(+) T cells were obtained from 20 patients treated with lamivudine or telbivudine for chronic hepatitis B at baseline, 12 weeks, and 24 weeks. The CDR3 spectratype of each T cell receptor (TCR) ? chain variable region (V?) gene family was analyzed, and the changes in the numbers of V? families with clonal expansions were compared in subjects with (n = 12) and without (n = 8) a virological response (52 week HBV DNA < 300 copies/ml). The number of CD8(+) TCR V? families with clonal expansions at 12 weeks relative to baseline (median [10th to 90th percentile], +2.5 [0 to +7] versus +1 [0 to +2], P = 0.03) and at 24 weeks relative to 12 weeks (+1 [0 to +2] versus -1 [-3 to +4], P = 0.006) was higher in subjects with a virological response versus subjects without a virological response, as were interleukin-2 (IL-2) but not IL-21 mRNA levels in peripheral blood mononuclear cells. The duration of new expansions at 12 weeks was higher (P < 0.0001) in responders. Increased numbers of CD8(+) T cell expansions after antiviral therapy are associated with a virological response to treatment. These CD8(+) T cells are a potential target for a therapeutic vaccine for chronic hepatitis B.
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[Metrx quadrant system in the surgery of lumbar spondylolisthesis: preliminary clinical application].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 10-29-2010
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To evaluate the clinical efficacy and minimal invasiveness of quadrant posterior distraction system in the posterior lumbar spondylolisthesis (LS) surgery.
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Nursing students experiences of their first encounter with death during clinical practice in Taiwan.
J Clin Nurs
PUBLISHED: 07-28-2010
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The aim of this study was to elucidate the experiences of first encountering death by nursing students during clinical practice. The objective is to assist nursing educational and clinical professionals to provide essential assistance for nursing students who encounter patient death.
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Growth suppression of cervical carcinoma by pigment epithelium-derived factor via anti-angiogenesis.
Cancer Biol. Ther.
PUBLISHED: 06-08-2010
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Pigment epithelium-derived factor (PEDF), an angiogenesis inhibitor with multiple other functions, balances angiogenesis in the eye and blocks tumor progression. Cervical cancer, an angiogenesis-dependent tumor, is the second most common cancer in women without effective treatment. It has been reported that PEDF can inhibit several types of tumors, however, the potential of PEDF for the treatment of cervical carcinoma has not been well explored. The present study was designed to investigate the effect of recombinant PEDF on the neovascularization and growth of cervical carcinoma. We found for the first time that PEDF was downregulated apparently in human cervical carcinoma nests compared to either normal cervical epithelium or nonneoplastic peritumoral epithelium, suggesting potential anti-angiogenesis function by supplement of PEDF in cervical carcinoma. Intraperitoneal injection of PEDF in xenografted cervical carcinoma mice suppressed tumor growth with 68% reduction. Microvessel density in tumor tissues treated with PEDF was significantly decreased. PEDF dose-dependently inhibited proliferation and induced apoptosis of endothelial cells, but had no direct effect on proliferation and apoptosis of Hela cells under both normoxia and hypoxia. These results suggested that PEDF suppressed tumor growth by blocking angiogenesis instead of a direct cytotoxic effect on tumor cells. VEGF, a major angiogenic stimulator, was downregulated by PEDF in Hela cells by downregulation of HIF-1?, a crucial transcriptional factor for VEGF expression. Downregulation of VEGF expression in tumor cells through inhibiting HIF-1?, thus attenuating the paracrine effect of VEGF on endothelial cells, may represent a mechanism for the anti-angiogenic activity of PEDF.
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A variant of estrogen receptor-alpha, ER-alpha36 is expressed in human gastric cancer and is highly correlated with lymph node metastasis.
Oncol. Rep.
PUBLISHED: 06-02-2010
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Gastric cancer is one of the most common cancers in the world; almost two-thirds of gastric cancer cases and deaths occur in less developed regions. The molecular and cellular events during development of gastric cancer remain unclear. Herein, we examined the expression of ER-alpha36, an ER-alpha variant, in established gastric cancer cell lines and specimens from 22 gastric cancer patients. RT-PCR, Western blot and immunohistochemistry methods were used to assess expression levels of ER-alpha36. ER-alpha36 localization in gastric cancer cells was determined with an immunofluorenscence assay. Both mRNA and protein of ER-alpha36 were detected in all established gastric cancer cell lines examined. Higher levels of ER-alpha36 mRNA were expressed in 17 of 22 (77.27%) tumor specimens examined compared to the paired normal tissues (p<0.05). ER-alpha36 protein was expressed mainly on the plasma membrane and in the cytoplasm of the established gastric cancer cells. ER-alpha36 expression is highly correlated with lymph node metastasis in human gastric cancer (P<0.05). The estrogen receptor variant ER-alpha36 is highly expressed in human gastric cancer. ER-alpha36 expression may be used as a predictive marker for lymph node metastasis of gastric cancer.
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G-proteins modulate invertebrate synaptic calcium channel (LCav2) differently from the classical voltage-dependent regulation of mammalian Cav2.1 and Cav2.2 channels.
J. Exp. Biol.
PUBLISHED: 06-01-2010
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Voltage-gated calcium channels in the Ca(v)2 channel class are regulators of synaptic transmission and are highly modified by transmitter inputs that activate synaptic G-protein-coupled receptors (GPCRs). A ubiquitous form of G-protein modulation involves an inhibition of mammalian Ca(v)2.1 and Ca(v)2.2 channels by Gbetagamma dimers that can be relieved by high-frequency trains of action potentials. Here, we address whether the ubiquitous and versatile form of G-protein regulation in mammals is also found in simpler invertebrate nervous systems. Remarkably, the invertebrate LCa(v)2 channel from the pond snail, Lymnaea stagnalis, does not bear any of the hallmarks of mammalian, voltage-dependent G-protein inhibition of Ca(v)2.2. Swapping either the I-II linker or N-terminus of Ca(v)2.2, which serve as key binding domains for G-protein inhibition, does not endow invertebrate LCa(v)2 channels with voltage-dependent G-protein modulatory capacity. Instead, in vitro expressed LCa(v)2 channels are inhibited slowly by the activation of cAMP, in a manner that depends on G-proteins but does not depend on Gbetagamma subunits. A similar G-protein and cAMP-dependent inhibition of nifedipine-insensitive LCa(v)2 currents is also consistent in native and identified Lymnaea VD4 neurons. The slower inhibition using a cellular messenger such as cAMP may meet the modulatory needs in invertebrates while an activity-dependent regulation, evolving in vertebrates, provides a more dynamic, fine-tuning of neurosecretion by regulating the influence of neurotransmitter inputs through presynaptic GPCRs.
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The functions of hospital-based home care for people with severe mental illness in Taiwan.
J Clin Nurs
PUBLISHED: 05-27-2010
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The purposes of this study were to understand the functions of hospital-based home care for people with severe mental illness in Taiwan, and the factors that affect functions of professionals who provide hospital-based home care.
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A theory of meaning of caregiving for parents of mentally ill children in Taiwan, a qualitative study.
J Clin Nurs
PUBLISHED: 05-27-2010
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The aim of this study is to generate a theory of meaning of care-giving for parents of mentally ill children in Taiwan.
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Impact of Taiwanese culture on beliefs about expressing anxiety and engaging in physical activity: a discursive analysis of the literature.
J Clin Nurs
PUBLISHED: 05-25-2010
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This paper analysed the scholarly discourse about the influence of Taiwanese culture on beliefs about expressing anxiety and engaging in physical activity from literature review.
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Gold-catalyzed synthesis of nitrogen-containing heterocycles from epsilon-N-protected propargylic esters.
Org. Biomol. Chem.
PUBLISHED: 04-27-2010
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A mild and efficient gold-catalyzed tandem cyclization to piperidinyl enol esters has been developed with facilely available epsilon-N-Boc-protected propargylic esters.
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[Characteristics of CDR3 of TCR beta on CD8+ T cells in chronic hepatitis B patients].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 04-13-2010
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To analyze the characteristics of CDR3 of TCRbeta on CD8+ T cells in chronic hepatitis B patients.
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Nucleophilic cationization reagents.
Tetrahedron Lett.
PUBLISHED: 03-06-2010
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Nucleophilic cationization reagents fitted with aminooxy groups are described. Practical syntheses of mono- and bis-aminooxy tetraalkylammonium iodides including N-hydroxyethyl-functionalized analogs are reported. An oximation example using one of the reagents is presented to illustrate their use in synthesis of cationic materials.
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A grounded theory study of action/interaction strategies used when Taiwanese families provide care for formerly suicidal patients.
Public Health Nurs
PUBLISHED: 11-12-2009
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There is a dearth of evidence on the care that families provide to their relatives after they have been discharged from hospital following an attempted suicide. The aim of this study was to explore ex-patients and family caregivers perceptions of the care provided at home following hospital discharge.
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Poly[diaqua-bis(?(3)-1H-benzimidazole-5,6-dicarboxyl-ato-?N:O,O:O)bis-(?(2)-1H,3H-benzimidazolium-5,6-dicarboxyl-ato-?O,O:O)digadolinium(III)].
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 10-19-2009
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In the title complex, [Gd(2)(C(9)H(4)N(2)O(4))(2)(C(9)H(5)N(2)O(4))(2)(H(2)O)(2)](n), two of the benzimidazole-5,6-dicarboxyl-ate ligands are pro-ton-ated at the imidazole groups. Each Gd(III) ion is coordinated by six O atoms and one N atom from five ligands and one water mol-ecule, displaying a distorted bicapped trigonal-prismatic geometry. The Gd(III) ions are linked by the carboxyl-ate groups and imidazole N atoms, forming a layer parallel to (001). These layers are further connected by O-H?O and N-H?O hydrogen bonds into a three-dimensional supra-molecular network.
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Poly[[diaqua-bis(?(2)-isonicotinato-?N:O)bis-(?(3)-isonicotinato-?N:O:O)neodymium(III)disilver(I)] nitrate monohydrate].
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 09-26-2009
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In the title complex, {[Ag(2)Nd(C(6)H(4)NO(2))(4)(H(2)O)(2)]NO(3)·H(2)O}(n), the Nd(III) ion is coordinated by eight O atoms from six isonicotinate ligands and two water mol-ecules in a distorted square anti-prismatic geometry. Each Ag(I) ion is coordinated by two N atoms from two different isonicotinate ligands. The crystal structure exhibits a two-dimensional heterometallic polymeric layer. O-H?O hydrogen bonds involving the coordinated and uncoordinated water mol-ecules and intra-layer ?-? inter-actions between the pyridine rings [centroid-centroid distances = 3.571?(2) and 3.569?(2)?Å] are observed. Each layer inter-acts with two neighboring ones via Ag?O(H(2)O) contacts and inter-layer ?-? inter-actions [centroid-centroid distances = 3.479?(3) to 3.530?(3)?Å], leading to a three-dimensional supra-molecular network.
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Hospital-based home care for people with severe mental illness in Taiwan: a substantive grounded theory.
J Clin Nurs
PUBLISHED: 09-03-2009
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The purpose of this study is to generate a substantive theory of hospital-based home care for people with severe mental illness in Taiwan.
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The lived experiences of brokered brides who have attempted suicide in Taiwan.
J Clin Nurs
PUBLISHED: 07-20-2009
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The purpose of this study was to explore the lived experiences of brokered brides who have attempted suicide in Taiwan.
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Parent-child acculturation, parenting, and adolescent depressive symptoms in Chinese immigrant families.
J Fam Psychol
PUBLISHED: 07-10-2009
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Using a sample of 388 father-adolescent and 399 mother-adolescent dyads in Chinese immigrant families, the current investigation tested Portes and Rumbauts (1996) assertion that generational dissonance may indicate a family context that places children at increased risk for adverse outcomes. Study findings suggest that a high discrepancy in father-adolescent acculturation levels relates significantly to more adolescent depressive symptoms. The study further demonstrates that the quality of the parenting relationship between fathers and adolescents operates as a mediator between father-adolescent acculturation discrepancy and adolescent depressive symptoms. Specifically, a high level of discrepancy in American orientation between fathers and adolescents is associated with unsupportive parenting practices, which, in turn, are linked to more adolescent depressive symptoms. These relationships are significant even after controlling for the influence of family socioeconomic status and parents and adolescents sense of discrimination within the larger society.
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A Chinese version of the Meaning in Caregiving Scale: an assessment of its reliability and validity.
Perspect Psychiatr Care
PUBLISHED: 04-16-2009
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To evaluate the reliability and construct validity of the Meaning in Caregiving Scale (MICS) by confirmatory factor analysis and to explore whether the MICS meets the goodness-of-fit criteria for the 3-factor model.
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Enhanced cellular uptake of chlorine e6 mediated by stearic acid-grafted chitosan oligosaccharide micelles.
J Drug Target
PUBLISHED: 04-04-2009
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Chlorines are attractive compounds for photodynamic therapy because of their high absorption in the red wavelength region. The stearic acid-grafted chitosan oligosaccharide (CSO-SA) micelles have been presented as potential candidates for intracellular drug delivery carrier because of their special structure. In this study, CSO-SA micelles were prepared to encapsulate chlorine e6 (Ce6). The physicochemical properties of synthesized CSO-SA micelles were characterized. The critical micelle concentration (CMC) of CSO-SA with 4.96% amino substituted degree (SD %) was about 36.27 +/- 1.51 microg/mL. The Ce6-loaded CSO-SA micelles were then prepared by a dialysis method, and the properties and drug release profiles of Ce6- loaded CSO-SA micelles (CSO-SA/Ce6) were investigated. The loading of Ce6 in the CSO-SA micelles could reach higher drug encapsulation efficiency (%), which was approximately 100%. The size of CSO-SA/Ce6 decreased after the loading of Ce6. The zeta potential of CSO-SA/Ce6 and the drug release rate decreased with the loading content of drug. After the Ce6 molecules were encapsulated into the micelles of CSO-SA, the cellular uptake percentage of Ce6 was much more than that of the free drug. And the cellular uptake percentage of CSO-SA/Ce6 micelles was increased with the incubation time in a short period.
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A novel single-cell quantitative real-time RT-PCR method for quantifying foot-and-mouth disease viral RNA.
J. Virol. Methods
PUBLISHED: 03-19-2009
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Foot-and-mouth disease virus is a positive-sense, single-stranded RNA virus with a negative strand as its replication intermediate, which can cause severe acute infection in sensitive cell lines. To investigate better the actual state of virus infection, there is a need to measure the amount of FMDV RNA in a single acutely infected cell rather than in a large number of cells. Therefore, in the present study, a strand-specific single-cell quantitative real-time RT-PCR was developed to analyze the RNA or FMDV. This new method uses two techniques in concert with each other: a technique for isolating single cells with micromanipulators, which is coupled to an assay for detecting viral RNA by real-time RT-PCR. In the assay of acute infection, 185 of 224 (82.6%) single-cell samples were positive and contained viral genome copies ranging from several to thousands, and up to 1,000,000 copies. However, not all cells were infected and there were differences in the number of viral RNA copies between cells. A single-cell quantitative RT-PCR was validated to be feasible and effective.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.