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Find video protocols related to scientific articles indexed in Pubmed.
Sulfate ion (SO4(2-)) release from old and new cation exchange resins used in condensate polishing systems for power plants.
Water Sci. Technol.
PUBLISHED: 10-18-2014
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In this study, a dynamic cycle test, a static immersion method and a pyrolysis experiment were combined to examine the characteristics of SO4(2-) released from several new and old cation exchange resins used in condensate polishing systems for power plants. The results show that the quantity and velocity of SO4(2-) released from new and old resins tend to balance in a short time during the dynamic cycle experiment. SO4(2-) is released by 1500H (monosphere super gel type cation exchange resins) and 001 × 7 (gel type cation exchange resins) new and old cation exchange resins, the quantity of which increases according to immersion time. In the pyrolysis experiment, the quantity of SO4(2-) released from resins increases and the pH of the pyrolysis solution transforms from alkaline to acidic with an increase in temperature.
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[Expression of CD20 in B-cell precursor acute lymphoblastic leukemia and its correlation with clinical outcomes].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 10-08-2014
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To determine whether expression of CD20 is associated with clinical outcomes of childhood B-cell precursor acute lymphoblastic leukemia (BCP-ALL).
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[Dynamic accumulation regulation of curcumin, demethoxycurcumin and bisdemethoxyeurcumin in three strains of curcuma longae rhizome].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 10-03-2014
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The paper is aimed to study the dynamic accumulation regulation of curcumin (Cur), demethoxycurcumin (DMC) and bisdemethoxyeurcumin (BDMC) in three strains of Curcuma longa, and provide scientific references for formalized cultivation, timely harvesting, quality control and breeding cultivation of C. longa. The accumulation regulation of the three curcumin derivatives was basically the same in rhizome of three strains. The relative contents decreased along with plant development growing, while the accumulation per hectare increased with plant development growing. The accumulation of curcuminoids per hectare could be taken as the assessment standard for the best harvest time of C. longa. A3 was the best strain in terms of Cur and BDMC content.
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[Investigation on operation timing of limb fractures combined with severe craniocerebral trauma in children].
Zhongguo Gu Shang
PUBLISHED: 09-23-2014
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To investigate the best choice of operation opportunity and operation plan for limb fractures combined with severe craniocerebral trauma in children.
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Clinical Characteristics of Severe Congenital Neutropenia caused by Novel ELANE Gene Mutations.
Pediatr. Infect. Dis. J.
PUBLISHED: 08-26-2014
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Mutations within the ELANE gene, which encodes human neutrophil elastase, are the most common genetic causes of severe congenital neutropenia (SCN). No cases of SCN have been previously described from a Chinese population. Herein, we describe the clinical, hematologic, and molecular characteristics of seven Chinese SCN cases with novel ELANE mutations.
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[Rice endogenous nitrogen fixing and growth promoting bacterium Herbaspirillum seropedicae DX35].
Wei Sheng Wu Xue Bao
PUBLISHED: 07-03-2014
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To screen efficient nitrogen fixation endophytes from rice and to analyze their growth-promoting properties.
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Cost-effective, transfer-free, flexible resistive random access memory using laser-scribed reduced graphene oxide patterning technology.
Nano Lett.
PUBLISHED: 05-12-2014
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Laser scribing is an attractive reduced graphene oxide (rGO) growth and patterning technology because the process is low-cost, time-efficient, transfer-free, and flexible. Various laser-scribed rGO (LSG) components such as capacitors, gas sensors, and strain sensors have been demonstrated. However, obstacles remain toward practical application of the technology where all the components of a system are fabricated using laser scribing. Memory components, if developed, will substantially broaden the application space of low-cost, flexible electronic systems. For the first time, a low-cost approach to fabricate resistive random access memory (ReRAM) using laser-scribed rGO as the bottom electrode is experimentally demonstrated. The one-step laser scribing technology allows transfer-free rGO synthesis directly on flexible substrates or non-flat substrates. Using this time-efficient laser-scribing technology, the patterning of a memory-array area up to 100 cm(2) can be completed in 25 min. Without requiring the photoresist coating for lithography, the surface of patterned rGO remains as clean as its pristine state. Ag/HfOx/LSG ReRAM using laser-scribing technology is fabricated in this work. Comprehensive electrical characteristics are presented including forming-free behavior, stable switching, reasonable reliability performance and potential for 2-bit storage per memory cell. The results suggest that laser-scribing technology can potentially produce more cost-effective and time-effective rGO-based circuits and systems for practical applications.
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Effects of resveratrol on gut microbiota and fat storage in a mouse model with high-fat-induced obesity.
Food Funct
PUBLISHED: 04-11-2014
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Recent studies have investigated the anti-obesity effect of resveratrol, but the pathways through which resveratrol resists obesity are not clear. In the present study, we hypothesize that resveratrol exerts anti-obesity effects that are likely mediated by mechanisms of regulating gut microbes, and in turn, improving fat storage and metabolism. Gut microbes, glucose and lipid metabolism in high-fat diet (HF) mice in vivo are investigated after resveratrol treatment. Several biochemical markers are measured. Fluorescence in situ hybridization and flow cytometry are used to monitor and quantify the changes in gut microbiota. The key genes related to fat storage and metabolism in the liver and visceral adipose tissues are measured by real-time PCR. The results show that resveratrol (200 mg per kg per day) significantly lowers both body and visceral adipose weights, and reduces blood glucose and lipid levels in HF mice. Resveratrol improves the gut microbiota dysbiosis induced by the HF diet, including increasing the Bacteroidetes-to-Firmicutes ratios, significantly inhibiting the growth of Enterococcus faecalis, and increasing the growth of Lactobacillus and Bifidobacterium. Furthermore, resveratrol significantly increases the fasting-induced adipose factor (Fiaf, a key gene negatively regulated by intestinal microbes) expression in the intestine. Resveratrol significantly decreases mRNA expression of Lpl, Scd1, Ppar-?, Acc1, and Fas related to fatty acids synthesis, adipogenesis and lipogenesis, which may be driven by increased Fiaf expression. The Pearson's correlation coefficient shows that there is a negative correlation between the body weight and the ratios of Bacteroidetes-to-Firmicutes. Therefore, resveratrol mediates the composition of gut microbes, and in turn, through the Fiaf signaling pathway, accelerates the development of obesity.
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Clinical, molecular, and T cell subset analyses in a small cohort of Chinese patients with hyper-IgM syndrome type 1.
Hum. Immunol.
PUBLISHED: 03-10-2014
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Type 1 hyper-IgM syndrome (HIGM1) is a rare primary immunodeficiency disease caused by mutations in the CD40L gene. Patients often present with recurrent infections and autoimmune manifestations. We investigated the clinical and molecular characteristics of HIGM1 in thirteen patients from the Chinese mainland and examined the proportion of CD4(+)CD25(+)FoxP3(+)Treg, Th17, and Th1 cells in the peripheral blood. We identified ten distinct CD40L mutations in eleven patients: one missense mutation, one nonsense mutation, one insertion mutation (in frame), and seven deletions. Six of these mutations were novel. We observed the percentage of Tregs in the peripheral blood of HIGM1 patients decreased markedly compared with that in healthy controls, but no statistically significant differences was found in the percentages of Th17 and Th1. The identified mutations reflect the heterogeneity of the CD40L gene in HIGM1. Precise genetic diagnosis of HIGM1 will enable appropriate therapeutic interventions, reliable detection of carriers, and genetic counseling. Skewed Treg, Th17/Treg, and Th1/Treg profiles may be associated with immune responses to autoimmunity or infection, which requires replication in larger studies.
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Relationship between different surgical methods, hemorrhage position, hemorrhage volume, surgical timing, and treatment outcome of hypertensive intracerebral hemorrhage.
World J Emerg Med
PUBLISHED: 03-03-2014
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The present study aimed to explore the relationship between surgical methods, hemorrhage position, hemorrhage volume, surgical timing and treatment outcome of hypertensive intracerebral hemorrhage (HICH).
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Clinical and molecular features of 38 children with chronic granulomatous disease in mainland china.
J. Clin. Immunol.
PUBLISHED: 01-25-2014
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Chronic granulomatous disease (CGD) is an inherited disorder, with phagocytes failing to produce antimicrobial superoxide due to deficient NADPH oxidase activity. Mutations in the gene encoding CYBB are responsible for the majority of the CGD cases. To date, there have been no reports on large samples of children with CGD in China. Therefore, in this study, we described the clinical and molecular features of 38 suspected CGD patients from 36 unrelated Chinese families.
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Rhizobium rhizoryzae sp. nov., isolated from rice roots.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 01-21-2014
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Two strains (J3-AN59(T) and J3-N84) of Gram-stain-negative, aerobic and rod-shaped bacteria were isolated from the roots of fresh rice plants. The 16S rRNA gene sequence similarity results showed that the similarity between strains J3-AN59(T) and J3-N84 was 100?%. Both strains were phylogenetically related to members of the genus Rhizobium, and they were most closely related to Rhizobium tarimense ACCC 06128(T) (97.43?%). Similarities in the sequences of housekeeping genes between strains J3-AN59(T) and J3-N84 and those of recognized species of the genus Rhizobium were less than 90?%. The polar lipid profiles of both strains were predominantly composed of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unknown aminophospholipid. The major cellular fatty acids were summed feature 8 (C18?:?1?7c and/or C18?:?1?6c) and C16?:?0. The DNA G+C contents of J3-AN59(T) and J3-N84 were 55.7 and 57.1 mol%, respectively. The DNA-DNA relatedness value between J3-AN59(T) and J3-N84 was 89?%, and strain J3-AN59(T) showed 9?% DNA-DNA relatedness to R. tarimense ACCC 06128(T), the most closely related strain. Based on this evidence, we found that J3-AN59(T) and J3-N84 represent a novel species in the genus Rhizobium and we propose the name Rhizobium rhizoryzae sp. nov. The type strain is J3-AN59(T) (?=?ACCC 05916(T)?=?KCTC 23652(T)).
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Changes in resistant starch from two banana cultivars during postharvest storage.
Food Chem
PUBLISHED: 01-15-2014
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Banana resistant starch samples were extracted and isolated from two banana cultivars (Musa AAA group, Cavendish subgroup and Musa ABB group, Pisang Awak subgroup) at seven ripening stages during postharvest storage. The structures of the resistant starch samples were analysed by light microscopy, polarising microscopy, scanning electron microscopy, X-ray diffraction, and infrared spectroscopy. Physicochemical properties (e.g., water-holding capacity, solubility, swelling power, transparency, starch-iodine absorption spectrum, and Brabender microviscoamylograph profile) were determined. The results revealed significant differences in microstructure and physicochemical characteristics among the banana resistant starch samples during different ripening stages. The results of this study provide valuable information for the potential applications of banana resistant starches.
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A novel mutation in the TECTA gene in a Chinese family with autosomal dominant nonsyndromic hearing loss.
PLoS ONE
PUBLISHED: 01-01-2014
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TECTA-related deafness can be inherited as autosomal-dominant nonsyndromic deafness (designated DFNA) or as the autosomal-recessive version. The ?-tectorin protein, which is encoded by the TECTA gene, is one of the major components of the tectorial membrane in the inner ear. Using targeted DNA capture and massively parallel sequencing (MPS), we screened 42 genes known to be responsible for human deafness in a Chinese family (Family 3187) in which common deafness mutations had been ruled out as the cause, and identified a novel mutation, c.257-262CCTTTC>GCT (p. Ser86Cys; p. Pro88del) in exon 3 of the TECTA gene in the proband and his extended family. All affected individuals in this family had moderate down-sloping hearing loss across all frequencies. To our knowledge, this is the second TECTA mutation identified in Chinese population. This study demonstrates that targeted genomic capture, MPS, and barcode technology might broaden the availability of genetic testing for individuals with undiagnosed DFNA.
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[Expression and significance of interleukin-6, interferon-inducible protein-10 and interleukin-17 in serum and synovial fluid of patients with juvenile idiopathic arthritis].
Zhonghua Er Ke Za Zhi
PUBLISHED: 10-15-2013
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To detect the disparity of three cytokines interleukin-6 (IL-6), interferon-inducible protein 10 (IP-10) and interleukin-17 (IL-17) in peripheral blood (PB) and synovial fluid (SF) of patients with juvenile idiopathic arthritis (JIA).
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[Analysis on coverage rate of primary immunization of measles vaccine among migrant children in Yiwu, Zhejiang province from 2007 to 2010].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 10-12-2013
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To investigate the coverage rate of primary immunization of measles containing vaccine (MCV1) among migrant children in Yiwu,Zhejiang province.
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Expression and significance of HIF-1? in pulmonary fibrosis induced by paraquat.
Exp. Biol. Med. (Maywood)
PUBLISHED: 09-20-2013
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It is commonly accepted that epithelial-mesenchymal transition contributes to fibrotic remodeling, but the molecular pathways involved in paraquat (PQ)-induced epithelial-mesenchymal transition remain uncharacterized. The objective of this study was to evaluate the potential involvement of HIF-1? in TGF-?1/?-Catenin and Snail pathway after PQ poisoning. In our study, 86 Spragne-Dawley rats were randomly divided into control group and PQ group, which received intragastric infusion of 20% PQ solution 50?mg/kg. Rats in the PQ group were subsequently divided into eight subgroups (10 for each subgroup) and samples were collected at different predetermined time points (2, 6, 12, 24, 48, 72, 96?h and 7?d). Fibrosis markers, including ?-catenin, snail and ?-SMA, were measured by western blot. The activity of HIF-1? was determined by western blot and immunofluorescence. We found that in PQ-induced pulmonary fibrosis, the level of PaO2 was significantly reduced in the 6-h subgroup, when compared to the control group (P?
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[Silencing hypoxia inducible factor-2? gene by small interference RNA inhibits the growth of mammosphere cells in nude mice under hypoxic microenvironment].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 08-02-2013
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To explore the effects of silencing hypoxia inducible factor-2? (HIF-2?) by small interference RNA on the growth of mammosphere cells in nude mice under hypoxic microenvironment.
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Proteomic analysis in peritoneal dialysis patients with different peritoneal transport characteristics.
Biochem. Biophys. Res. Commun.
PUBLISHED: 07-22-2013
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Peritoneal membranes can be categorized as high, high average, low average, and low transporters, based on the removal or transport rate of solutes. In this study, we used proteomic analysis to determine the differences in proteins removed by different types of peritoneal membranes. Peritoneal transport characteristics in patients who received peritoneal dialysis therapy were assessed by a peritoneal equilibration test. Two-dimensional differential gel electrophoresis technology followed by quantitative analysis was performed to study the variation in protein expression from peritoneal dialysis effluents (PDE) among different groups. Proteins were identified by MALDI-TOF-MS/MS analyses. Further validation in PDE or serum was performed utilizing ELISA analysis. Proteomics analysis revealed ten protein spots with significant differences in intensity levels among different groups, including vitamin D-binding protein, complement C3, apolipoprotein-A1, complement factor C4A, haptoglobin, alpha-1 antitrypsin, immunoglobulin kappa light chain, alpha-2-microglobulin, retinol-binding protein 4 and transthyretin. The levels of vitamin D-binding protein, complement C3, and apolipoprotein-A1 in PDE derived from different groups were greatly varied (P<0.05). However, no significant difference was found in the serum levels of these proteins among different groups (P>0.05 for all groups). This study provides a novel overview of the differences in PDE proteomes of four types of peritoneal membranes. Vitamin D-binding protein, complement C3, and apolipoprotein-A1 showed enhanced expression in PDE of patients with high transporter.
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[Clinical characteristics of pediatric victims in the Lushan and Wenchuan earthquakes and experience of medical rescue].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 06-25-2013
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To get a more comprehensive understanding of the clinical characteristics of pediatric victims in earthquake and to summarize the experience of medical rescue.
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[Diversity of endophytic bacteria in rice seeds and their secretion of indole acetic acid].
Wei Sheng Wu Xue Bao
PUBLISHED: 05-18-2013
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This study aimed to investigate the diversity of endophytic bacteria isolated from rice seeds, and screen indole acetic acid secrecting srtains.
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Distribution, clinical features and molecular analysis of primary immunodeficiency diseases in Chinese children: a single-center study from 2005 to 2011.
Pediatr. Infect. Dis. J.
PUBLISHED: 05-16-2013
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Two hundred three children with genetically proven primary immunodeficiency diseases (PIDs) from 197 unrelated families were enrolled from January 2005 to December 2011.
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[Expression of breast cancer resistance protein and p-glycoprotein in residual breast cancer tissue after chemotherapy and its correlation with cancer stem cells].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 05-07-2013
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To compare the expression differences of breast cancer resistance protein(BCRP/ABCG2) and P-glycoprotein(P-gp) in breast cancer tissue before chemotherapy and in residual breast cancer tissue, and to explore its correlation with breast cancer stem cells.
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Successful interventional treatment of post-biopsy renal artery pseudoaneurysm in pediatric patients.
Clin. Nephrol.
PUBLISHED: 04-27-2013
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With an incidence ranging from 0.01% to 1.0%, renal artery pseudoaneurysm (RAP) is a rare complication after renal biopsy, percutaneous renal surgery, penetrating trauma, and rarely blunt renal trauma.
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Repeated pulse intramuscular injection of pralidoxime chloride in severe acute organophosphorus pesticide poisoning.
Am J Emerg Med
PUBLISHED: 03-05-2013
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This study aimed to clarify the efficacy of 2 therapies for patients with severe acute organophosphorus pesticide poisoning, including atropine adverse effects, the length of intensive care unit (ICU) stay, complications, and mortality.
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Effects of vitamin E on expressions of eight microRNAs in the liver of Nile tilapia (Oreochromis niloticus).
Fish Shellfish Immunol.
PUBLISHED: 02-27-2013
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Currently, microRNAs (miRNAs) are known to regulate cellular processes such as apoptosis, differentiation, cell cycle, and immune functions, and their expression can be altered by distinct stress conditions, such as oxidative stress. In immune systems of fish, vitamin E (VE) has a defined role as an antioxidant. In order to understand the molecular mechanism of vitamin E defending from oxidative stress, three groups of juvenile Nile tilapia (Oreochromis niloticus) (initial weight 3.25 ± 0.02 g) were fed to satiation with 3 semi-purified diets containing VE (DL-?-tocopherol acetate) of 0, 50, and 2500 mg/kg supplementation, respectively, with the expressions of eight miRNAs (miR-21, miR-223, miR-146a, miR-125b, miR-181a, miR-16, miR-155 and miR-122) in the liver of tilapia subsequently detected after 8-week growth experiment. Results showed that VE-deficient (0 mg/kg supplementation) decreased the activity of superoxide dismutase (SOD), and decreased the expressions of miR-223, miR-146a, miR-16 and miR-122, while excessive supplementation of VE (2500 mg/kg) decreased SOD activity and increased the expressions of all the eight miRNAs. The targets of the eight miRNAs were further predicated with bioinformatic approach and the possible regulating mechanisms of VE via miRNAs were analyzed. The present study confirmed that the differences in dietary VE affected expression of hepatic miRNAs which may partly demonstrate the molecular mechanism of VE, and the new idea of introducing miRNAs into research will provide the basic data for researches of molecular nutrition.
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[Hypoxia-inducible factor-1? expression in renal tissue of rats with paraquat poisoning].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 02-26-2013
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To observe the pathological changes of renal tissue in the rats with paraquat (PQ) poisoning as well as the serum creatinine (SCr) levels and expression levels of hypoxia-inducible factor-1? (HIF-1?) and transforming growth factor-? (TGF-bgr;) in renal tissue at different time points after PQ poisoning, and to investigate the association of HIF-1? with renal injury after PQ poisoning.
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Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma.
Mol Med Rep
PUBLISHED: 02-07-2013
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The aim of the present study was to investigate the protein expression of DNA methyltransferases (DNMTs) and genomic DNA methylation status of genomes in gastric signet ring cell carcinoma (SRC). Immunohistochemistry was performed to analyze DNMT expression and methylated DNA immunoprecipitation microarray (MeDIP?chip) and MeDIP quantitative real?time PCR (MeDIP?qPCR) were performed to analyze the genomic DNA methylation status in gastric SRC tissue. An increase in DNMT1 and decrease in DNMT3A expression in SRC tissue was observed compared with matched non?cancerous tissue. However, expression of other DNMTs, DNMT2, DNMT3B and DNMT3L, was not found to differ significantly between carcinoma and control. The MeDIP?chip assay revealed that methylation of gene promoters and CpG islands in SRC was higher than those in matched control tissue. However, MeDIP?qPCR analysis demonstrated that specific tumor?related genes, including ABL2, FGF18, TRAF2, EGFL7 and RAB33A were aberrantly hypomethylated in SRC tissue. Results of the current study indicate that gastric SRC may produce complex patterns of aberrant DNA methylation and DNMT expression.
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Bioactive eudesmane and germacrane derivatives from Inula wissmanniana Hand.-Mazz.
Phytochemistry
PUBLISHED: 01-23-2013
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Phytochemical investigation of Inula wissmanniana Hand.-Mazz. afforded 21 eudesmane and germacrane derivatives, including rare 4,5-secoeudesman-12,5-olide, eudesman-12,5-olide, 3,4-secoeudesman-12-oic acid, and germacra-4-en-12,6-olides. Their structures were elucidated by combinative analyses of MS, NMR, electronic circular dichroism, and X-ray crystallography data. Moreover, most of the isolates exhibited inhibition against lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophages and cytotoxicity in HepG2, PC-3, and MGC-803 tumor cells.
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Clinical features and mutation analysis of X-linked agammaglobulinemia in 20 Chinese patients.
World J Pediatr
PUBLISHED: 01-18-2013
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X-linked agammagobulinemia (XLA) is a primary immunodeficiency caused by Brutons tyrosine kinase (BTK) gene mutation. XLA patients have an extremely small amount of peripheral B cells and profound deficiency in all immunoglobulin isotypes. We analyzed the clinical, immunologic, and molecular characteristics of children with XLA in an attempt to improve the diagnosis and treatment of XLA in China.
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Structural and antioxidant modification of wheat peptides modified by the heat and lipid peroxidation product malondialdehyde.
J. Food Sci.
PUBLISHED: 12-19-2011
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Wheat peptides, the biological active peptides derived from foods, has an array of biological actions, including antiobesity, antimicrobial, and angiotensin I-converting enzyme inhibitory effects in mammalian species. Recent studies showed that some wheat peptides may show the noteworthy antioxidant potency against the peroxidation of lipids or fatty acids, but the effect of oxidation on its antioxidant activities is unclear. In the present study, we demonstrate that heat and malandialdehyde (MDA)-oxidized wheat peptides lose its surface hydrophobicity and reducing power, and show a relatively lower free radical-scavenging activitiy in vitro. Those modifications also lead to gradual formation of aggregates in wheat peptides and induce more reactive oxygen species (ROS) production in vivo. These findings indicate that oxidation may influence the functional properties and directly alter the structure of wheat peptides, and lead to the loss of its antioxidant potency both in vitro and in vivo, thereby providing a novel explanation for some of the potential health risks proposed for oxidized food in human.
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Ethyl 6-methyl-3-(2-methyl-prop-1-en-yl)-2-oxo-4-phenyl-1,2,3,4-tetra-hydro-pyrimidine-5-carboxyl-ate.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 10-03-2011
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In the mol-ecule of the title compound, C(18)H(22)N(2)O(3), the dihydro-pyrimidinone ring adopts an envelope conformation. The dihedral angle between the phenyl ring and the mean plane through the enamine fragment is 86.04?(7)°. The mol-ecular conformation is stabilized by an intra-molecular C-H?O hydrogen bond. In the crystal, inter-molecular N-H?O hydrogen bonds link pairs of mol-ecules into centrosymmetric dimers.
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[Preparation and identification of monoclonal antibodies against Pla protein of Yersinia pestis].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
PUBLISHED: 09-13-2011
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To prepare the monoclonal antibody of Pla with recombinant Pla (rPla) by hybridoma cell technology, which will lay the foundation for related research work.
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Frequency of established cardiovascular disease and its risk factors in Chinese patients with systemic lupus erythematosus.
Clin. Rheumatol.
PUBLISHED: 09-12-2011
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Cardiovascular disease (CVD) is one of the most serious complications of systemic lupus erythematosus (SLE). This study investigated the frequency of established CVD and its associated risk factors in Chinese patients with SLE. A retrospective, cross-sectional analysis was conducted of 1,072 consecutive patients with SLE from a single center. Patients with CVD were identified on the basis of medical record documentation. Stepwise multivariate logistic regression was used to assess the risk factors of CVD. Of the 1,072 patients with SLE, 71 (6.6%) had CVD. Patients with CVD were older than patients without CVD (39.0 ± 15.6 vs. 31.9 ± 13.3 years; P < 0.001), the frequency of CVD in patients aged ?19 years, between 20 and 39 years, between 40 and 69 years, and greater than 60 years was increased by 3.4%, 5.5%, 9.2%, 20.4%, respectively. The frequency of CVD was higher in SLE patients with nephritis than without nephritis (7.6% vs. 3.8%; P = 0.026). Multivariate regression analyses confirmed that age ?60 years (OR = 5.098; 95% CI 1.333, 19.488), higher diastolic blood pressure (OR = 1.050; 95% CI 1.022, 1.078), higher serum creatinine levels (OR = 1.002; 95% CI 1.000, 1.003), and long-term use of glucocorticoids (OR = 1.005; 95% CI 1.000, 1.010) were risk factors for CVD. HDL-C levels (OR = 0.121; 95% CI 0.041, 0.358) were negatively associated with CVD in patients with SLE. Our data suggest that the frequency of CVD was high in Chinese patients with SLE, and independent risk factors for CVD were increased age, higher diastolic blood pressure, higher serum creatinine levels, long-term use of glucocorticoids, and lower HDL-C levels.
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[Synthesis of novel beta-aminoalcohols containing nabumetone moiety with potential antidiabetic activity].
Yao Xue Xue Bao
PUBLISHED: 07-15-2011
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Twenty five new beta-aminoalcohols containing nabumetone moiety were prepared via the reduction of potassium borohydride with a convenient and efficient procedure, starting from beta-aminoketones that have been synthesized by our group. Their chemical structures were determined by IR, MS, 1H NMR, 13C NMR, HR-MS and antidiabetic activities were screened in vitro. Preliminary results revealed that the antidiabetic activity of most beta-aminoalcohols were better than that of the corresponding beta-aminoketones. Although most compounds showed weak antidiabetic activity, the alpha-glucosidase inhibitory activity of compounds 5hd(1) and 5id(2) reached 74.37% and 90.15%, respectively, which were superior to the positive control. The relative peroxisome proliferator-activated receptor response element (PPRE) activity of five compounds were more than 60%, among them compound 5ca possessed the highest activity (112.59%). As lead molecules of antidiabetic agents, compounds 5hd(1), 5id(2) and 5ca deserve further study.
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[Analysis of post-thaw infused cell dose for predicting engraftment after unrelated cord blood transplantation].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 07-07-2011
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This study was purposed to investigate the role of post-thaw infused donor cells for predicting engraftment and hematopoietic reconstitution after unrelated cord blood transplantation (UCBT). The retrospective analysis was performed on clinical data of 97 children with malignant or non-malignant diseases received single unit UCBT from August 1999 to April 2010. The impact of pre-freezing and post-thaw cell dose of total nucleated cells (TNC), CD34(+) cells and colony-forming units-granulocyte/macrophage (CFU-GM) on engraftment and hematological recovery after UCBT was analyzed. Unrelated donors were from Guangzhou cord blood bank (GZCBB) entirely. The results indicated that the pre-freezing TNC (/kg) (mean ± SD: 7.65 × 10? ± 4.26 × 10?; median: 6.34 × 10?), CD34(+)cells (/kg) (mean ± SD: 4.64 × 10(5) ± 4.47 × 10?; median: 3.03 × 10?) and CFU-GM (/kg) (mean ± SD: 0.79 × 10? ± 1.09 × 10?; median: 0.57 × 10?) showed a good correlation with their post-thaw counterparts including TNC(/kg) (mean ± SD: 6.98 × 10? ± 4.12 × 10?; median: 6.00 × 10?), CD34(+)cells (/kg)(Mean ± SD: 6.86 × 10? ± 8.56 × 10?; Median: 4.17 × 10?), and CFU-GM (/kg) (mean ± SD: 0.52 × 10? ± 0.52 × 10?; median: 0.39 × 10?) (r = 0.952, p < 0.001; r = 0.794, p < 0.001; r = 0.478, p < 0.001). Either the pre-freezing or post-thaw number of infused CFU-GM was significant higher in patients who achieved engraftment (n = 70) than those who suffered graft failure (n = 22) (p = 0.023 and 0.011, respectively), but no significant difference of TNC and CD34(+) cells dose (pre-freezing or post-thaw) were found between these two groups. Pre-freezing CFU-GM, TNC, CD34(+) cell dose negatively correlated with the time of neutrophil engraftment (r = -0.285, p = 0.018; r = -0.396, p = 0.002; r = -0.373, p = 0.002), as well as the post-thaw number of TNC and CD34(+) cells (r = -0.260, p = 0.031; r = -0.483, p < 0.001), whereas only pre-freezing CD34(+) cells showed a significant correlation with platelet engraftment time (r = -0.352, p = 0.013). It is concluded that the CFU-GM amount is useful for predicting engraftment of UCBT, while pre-freezing hematopoietic cell doses show superior correlation with the speed of engraftment and hematopoietic reconstitution than their post-thaw counterparts in pediatric recipients, suggesting that it is essential to perform hematopoietic potency assay on each cord blood unit prior to listing or release for administration.
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[Apoptosis-inducing Effect of 8-Bromo-7-Methoxychrysin on K562 cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 07-07-2011
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This study was purposed to investigate the apoptosis-inducing effect of 8-bromo-7-methoxychrysin (BrMChR) on leukemia K562 cells as well as the variation of caspase-3 activity and phosphorylated Akt (p-Akt) expression of K562 cells during the process of apoptosis. MTT assay was used to determine the inhibitory effect of BrMChR on proliferation of K562 cells. Cell apoptosis was assayed by AO/EB staining under fluorescent microscope and flow cytometry with Annexin V-FITC/PI staining. The expression level of p-Akt was measured by Western blot. The results showed that BrMChR had the inhibitory effect on proliferation of K562 cells and could induce apoptosis of these cells in dose-dependent manner, and these effects were significantly stronger than ChR. After treatment of K562 cells with 3 µmol/L ChR for 12 hours, the apoptosis rate was only 3.68%, but the apoptosis rate of K562 cells treated with 3 µmol/L BrMChR was 21.8%. In the same time, the caspase-3 activity significantly increased (p < 0.05), but the expression of p-Akt was down-regulated (p < 0.01). It is concluded that BrMChR can induce apoptosis of K562 cells and with effect stronger than chR. P-Akt may participate in the apoptosis process of K562 cells induced by BrMChR.
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A genome-wide association study in Han Chinese identifies multiple susceptibility loci for IgA nephropathy.
Nat. Genet.
PUBLISHED: 07-06-2011
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We performed a two-stage genome-wide association study of IgA nephropathy (IgAN) in Han Chinese, with 1,434 affected individuals (cases) and 4,270 controls in the discovery phase and follow-up of the top 61 SNPs in an additional 2,703 cases and 3,464 controls. We identified associations at 17p13 (rs3803800, P = 9.40 × 10(-11), OR = 1.21; rs4227, P = 4.31 × 10(-10), OR = 1.23) and 8p23 (rs2738048, P = 3.18 × 10(-14), OR = 0.79) that implicated the genes encoding tumor necrosis factor (TNFSF13) and ?-defensin (DEFA) as susceptibility genes. In addition, we found multiple associations in the major histocompatibility complex (MHC) region (rs660895, P = 4.13 × 10(-20), OR = 1.34; rs1794275, P = 3.43 × 10(-13), OR = 1.30; rs2523946, P = 1.74 × 10(-11), OR = 1.21) and confirmed a previously reported association at 22q12 (rs12537, P = 1.17 × 10(-11), OR = 0.78). We also found that rs660895 was associated with clinical subtypes of IgAN (P = 0.003), proteinuria (P = 0.025) and IgA levels (P = 0.047). Our findings show that IgAN is associated with variants near genes involved in innate immunity and inflammation.
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Comparative proteomics and phosphoproteomics analyses of DHEA-induced on hepatic lipid metabolism in broiler chickens.
Steroids
PUBLISHED: 06-24-2011
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Dehydroepiandrosterone (DHEA) is a precursor of the adrenocorticosteroid hormones that are common to all animals, including poultry. The study described herein was undertaken to investigate the effect of DHEA on lipid metabolism in broiler chickens during embryonic development and to determine the regulatory mechanisms involved in its physiological action. Treatment group eggs were injected with 50mg DHEA diluted in 50 ?L dimethyl sulfoxide (DMSO) per kg, while control group eggs (arbor acres [AA] fertilized) were injected with 50 ?L DMSO per kg prior to incubation. Liver samples were collected on days 9, 14 and 19 of embryonic development as well as at hatching. Extracted proteins were analyzed by two dimensional gel electrophoresis (2-DE) in combination with western blotting for specific anti-phosphotyrosine. The differential spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) or MALDI-TOF-TOF-MS. Peptide mass fingerprinting (PMF) of the differentially-expressed proteins were performed using the MASCOT, Prospector or proFound server. Thirty-seven proteins and twenty-two tyrosine phosphorylation proteins were successfully identified. All 37 proteins and 22 tyrosine phosphorylation proteins exhibited a significant volume difference from the control group. These results demonstrated that DHEA increased the expression and level of tyrosine phosphorylation and sulfotransferase proteins in broilers (at pI 5.9), therefore promoting the biotransformation of DHEA. The expression of apolipoproteinA-I was increased in the DHEA treatment group, which facilitated the conversion of cholesterol to cholesterol esters. Also, DHEA increased the expression of peroxiredoxin-6 and its tyrosine phosphorylation protein levels, thus enhancing its anti-oxidative activity. Furthermore, pyruvate dehydrogenase expression was decreased and the level of its tyrosine phosphorylation proteins increased in the DHEA treatment group. Take together, those data indicate that DHEA reduces the supply of acetyl-CoA by inhibiting the activity of its target enzyme (i.e., pyruvate dehydrogenase), thus affecting both protein synthesis and phosphorylation level and decreasing fat deposition in broiler chickens during embryonic development, which could reflect a physiologically-relevant DHEA fat-reduction mechanism in the broiler chicken.
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Differences in muscle cellularity and flesh quality between wild and farmed Coilia nasus (Engraulidae).
J. Sci. Food Agric.
PUBLISHED: 06-09-2011
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Populations of Coilia nasus, an anadromous fish, have declined dramatically in the Yangtze River estuary and its associated lakes owing to excessive fishing and changes in aquatic ecology. Recently, the success of artificial breeding programmes and advanced methods of propagation have allowed great increases in production of this species. Thus, to gain a better understanding of the flesh quality of C. nasus, muscle cellularity and quality parameters of the flesh were studied in wild and farmed specimens.
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[Inhibitory effect of pyrrolidine dithiocarbamate combined with matrine on the growth of human hepatocellular carcinoma xenografts].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 05-19-2011
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To investigate the relationship between activation of nuclear factor-K-gene binding (NF-?B) and apoptosis induced by matrine(MT) in transplanted tumor of human hepatocellular carcinoma in nude mouse.
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[Experimental study on Buyang Huanwu decoction ([Chinese characters: see text]) for promoting functional recovery of crushed common peroneal nerve in rats].
Zhongguo Gu Shang
PUBLISHED: 04-14-2011
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To study the effects of Buyang Huanwu Decoction ([Chinese characters: see text]) on promoting functional recovery of crushed common peroneal nerve in rats.
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[Mutation screening of the F VIII gene in 10 hemophilia A families].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 04-05-2011
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To identify the F VIII gene mutations of patients and suspected female carriers in 10 Hemophilia A (HA) families, and to guide the prenatal diagnosis.
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The paleoAP3-type gene CpAP3, an ancestral B-class gene from the basal angiosperm Chimonanthus praecox, can affect stamen and petal development in higher eudicots.
Dev. Genes Evol.
PUBLISHED: 03-30-2011
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Wintersweet (Chimonanthus praecox), a basal angiosperm endemic to China, has high ornamental value for developing beautiful flowers with strong fragrance. The molecular mechanism regulating flower development in wintersweet remains largely elusive. In this project, we seek to determine the molecular features and expression patterns of the C. praecox paleoAP3-type gene CpAP3 and examine its potential role in regulating floral development via ectopic expression in Arabidopsis thaliana and Petunia hybrida. The expression of CpAP3 is tissue-specific, with the highest level in the tepals, moderate level in carpels, and weak levels in stamen and vegetative stem tissues. Its dynamic expression during flowering is associated with flower-bud formation. Ectopic expression of CpAP3 partially rescued stamen development in ap3 mutant Arabidopsis. Although no phenotypic effect has been observed in wild-type Arabidopsis, CpAP3 overexpression in petunia brought rich morphological changes and homeotic conversions to flowers, mainly involving disruption of petal and stamen development. Expressed in a broader range than those canonical B-function regulators, the ancestral B-class gene CpAP3 can affect petal and stamen development in higher eudicots. This gene also holds some bioengineering potential in creating novel floral germplasms.
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Overexpression of Forkhead Box M1 transcription factor and nuclear factor-?B in laryngeal squamous cell carcinoma: a potential indicator for poor prognosis.
Hum. Pathol.
PUBLISHED: 02-21-2011
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The Forkhead Box M1 transcription factor and nuclear factor-?B have been shown to play important roles in the development and progression of human cancers. However, the functional significance of Forkhead Box M1 transcription factor in laryngeal squamous cell carcinoma and the correlation between Forkhead Box M1 transcription factor and nuclear factor-?B remain unclear. In the current study, we have shown that Forkhead Box M1 transcription factor and nuclear factor-?B were significantly overexpressed in laryngeal squamous cell carcinoma tissues and precancerous lesions, compared with adjacent normal tissues (both P < .001). The overexpression of Forkhead Box M1 transcription factor was significantly associated with histologic differentiation (rs = 0.321, P = .002), T stage (rs = 0.276, P = .009), lymph node metastasis (rs = 0.266, P = .012), and clinical stage (rs = 0.272, P = .010); overexpression of nuclear factor-?B was significantly associated with T stage (rs = 0.404, P < .001), lymph node metastasis (rs = 0.293, P = .005), and clinical stage (rs = 0.425, P < .001). Overexpressions of both Forkhead Box M1 transcription factor and nuclear factor-?B were associated with worse overall survival (P = .041 and P < .001, respectively). Multivariate Cox regression analysis showed that T stage, lymph node metastasis, and nuclear factor-?B were independent prognostic factors for laryngeal squamous cell carcinoma (P = .038, P = .014, and P = .005, respectively). Furthermore, a significant correlation was observed between Forkhead Box M1 transcription factor and nuclear factor-?B (rs = 0.683, P < .001), indicating the potential direct or indirect interaction between them. In conclusion, our results suggest that overexpressions of Forkhead Box M1 transcription factor and nuclear factor-?B and the possible interaction between them may play important roles in the development and progression of laryngeal squamous cell carcinoma, and Forkhead Box M1 transcription factor and nuclear factor-?B may serve as useful prognostic markers for laryngeal squamous cell carcinoma.
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Clinical characteristics and mutation analysis of X-linked severe combined immunodeficiency in China.
World J Pediatr
PUBLISHED: 02-17-2011
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X-linked severe combined immunodeficiency (X-SCID) is a rare, life-threatening immune disorder, caused by mutations of the gene for the ?-chain (?c) of the interleukin-2 receptor, IL2RG. We analyzed the clinical, immunologic, and molecular characteristics of children with X-SCID, attempting to improve the diagnosis and treatment of X-SCID in China.
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Comparative in vivo antioxidant capacity of DL-2-hydroxy-4-methylthiobutanoic acid (HMTBA) and DL-methionine in male mice fed a high-fat diet.
J. Sci. Food Agric.
PUBLISHED: 01-05-2011
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In animal diets, methionine (Met) is considered to be the first limiting amino acid, and the activity of synthetic Met is typically added either as DL-methionine (DLM) or as DL-2-hydroxy-4-methylthiobutanoic acid (HMTBA). It has been demonstrated that HMTBA exhibits a higher antioxidant capability in vitro as compared to DLM. However, the difference in antioxidant capability between DLM and HMTBA in vivo is unknown.
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[Outbreak of cholera associated with consumption of soft-shelled turtles, Sichuan province, China, 2009].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 12-18-2010
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Vibrio cholera was extremely rare in Sichuan province(no cases in 2008). Any outbreak could indicate contamination through the food supply system. In July 2009, a hospital reported a cluster of 7 diarrhea patients;all attended the same banquet. One patient was confirmed to have Vibrio cholera (O139). We conducted this investigation to identify the source of this possible cholera outbreak.
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[Theoretical basis and application of evidence-based clinical pathway of Chinese medicine].
Zhongguo Zhong Xi Yi Jie He Za Zhi
PUBLISHED: 07-31-2010
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Based on the principle of management, evidence-based medicine, operational research and health economics, this essay addressed the theoretical basis of clinical pathway and its application of evidence-based Chinese medicine to practice. It could be taken as references for different health care institutions and organizations for development of clinical pathway.
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[The effect of ATRA-induced leukemic cell differentiation on Brd7 gene expression in leukemia cell lines].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 06-22-2010
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This study was purposed to investigate the relationship between brd7 gene and differentiation of leukemia cells and the role of brd7 gene in differentiation of leukemia cells. The HL-60 and K562 cell lines were induced by all-trans retinoic acid (ATRA) for 7 days, then the cell morphologic change was observed under inverted microscope with Wright-Giema staining, the expression level of CD11b was detected by flow cytometry for evaluating cell differentiation level, the expression changes of BRD7 protein before inducing differentiation and in process of cell differentiation were determined by Western blot. The results showed that ATRA could inhibit the proliferation and induce differentiation of HL-60 cells, but no differentiation in K562 cells was induced by ATRA. The level of CD11b expression in HL-60 cells was up-regulated gradually during ATRA-induced cell differentiation. The expression of BRD7 protein increased markedly along with differentiation of HL-60 cells towards granulocytes. However, BRD7 protein did not significantly alter in K562 cells in which inducing differentiation was not found. It is concluded that brd7 gene expression enhances as the HL-60 cells differentiate, underlying which the mechanism remains to clarify.
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[Study on the expression of vitamin D receptor in bone of immature rats of disuse osteoporosis].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 04-08-2010
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To study the expression of vitamin D receptor in bone of immobilized immature rat and explore the relation between disuse osteoporosis (DOP) and the expression of vitamin D receptor (VDR) in bone.
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[Expression of paxillin in breast cancer cell with high and low metastatic potentiality].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 04-08-2010
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To study the expression of paxillin in the two subgroups of human breast cancer cells with high and low metastatic potentialities and the effect of paxillin on tumor metastasis and adhesion.
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[Effects of two organic pollutants on biomarker system of fish Lateolabrax japonicus and the pollution assessment].
Huan Jing Ke Xue
PUBLISHED: 04-03-2010
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Responses of a select suite of protective enzymes in Lateolabrax japonicus including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), reduced glutathione (GSH) glutathione S-transferase (GST), nitric oxide synthase (iNOS) in liver tissue and brain acetylcholinesterase (AChE), which could work as sensitively biochemical biomarkers were analyzed when exposed to different concentrations of sodium dodecylbenzene sulfonate (SDBS) and benzo[a]pyrene (B[a]P) under controlled laboratory conditions. Results showed that: (1) The biochemical biomarkers included in this investigation, different responses occurred in the enzymatic activities when exposed to different pollutants: the activities of SOD, GST, Gpx and GSH content were significantly induced by B[a]P (p < 0.05) while others present little different as compared to the control. Differently, SDBS exposure greatly affected the activities of CAT, Gpx, iNOS and AChE while showed little effect on the other evaluated biomarkers. Gpx was the most sensitive biomarker that sensitively indicated the organic pollution stress. (2) The simultaneous assay on heat shock protein 70 (Hsp70) expression was conducted by flow cytometry (FCM) and result presented that Hsp 70 expression could be effectively induced by SDBS as compared to the control throughout the experiment; however, B[a]P obviously induced Hsp70 during the first 12 d and then decreased to the level of the control. (3) The evaluated method of biomarker system combined with principal component analysis (PCA) could effectively distinguish different pollutants under controlled laboratory conditions and might be a promising and warning method for the early assessment of environmental change exposed to different organic pollutions.
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A preliminary study on the medical expenditure of Chinese medicine and integrative medicine treatment for influenza A (H1N1) in the fever clinics.
Chin J Integr Med
PUBLISHED: 03-15-2010
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To analyze the effectiveness of Chinese medicine and integrated Chinese and Western medicine for influenza A (H1N1) in the fever clinics and its relevant expenditure.
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Apoptosis induced by yessotoxins in Hela human cervical cancer cells in vitro.
Mol Med Rep
PUBLISHED: 03-09-2010
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To investigate the apoptotic effects of an extract of red-tide algae, Protoceratium reticulatum, on HeLa cells, we used liquid chromatography-tandem mass spectrometry, optical microscopy, Hoechst 33342/propidine iodide staining, and DNA gel electrophoresis to analyze its constituents and toxicity, as well as rhodamine 123 staining to investigate changes in mitochondrial membrane potential. Analysis showed that the P. reticulatum extract contained the yessotoxins (YTXs) homo-YTX, 45-OH-YTX and 45-OH-homo-YTX. The results indicated that P. reticulatum extract negatively influences HeLa cells and induces their apoptosis.
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Protective roles of cornuside in acute myocardial ischemia and reperfusion injury in rats.
Phytomedicine
PUBLISHED: 02-18-2010
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Cornuside is a secoiridoid glucoside isolated from the fruit of Cornus officinalis SIEB. et ZUCC. In this study, we investigated the anti-myocardial ischemia and reperfusion (I/R) injury effects of cornuside in vivo and elucidated the potential mechanism. Rat models of myocardial I/R were induced by coronary occlusion followed by reperfusion or by Isoproterenol (ISO), treatment of rats with cornuside (20 and 40 mg/kg, i.v.) protected the animals from myocardial I/R injury as indicated by a decrease in infarct volume, improvement in hemodynamics and reduction of myocardial damage severity. Treatment with cornuside also attenuated polymorphonuclear leukocytes (PMNs) infiltration, decreased myeloperoxidase (MPO) activity in the heart, lowered serum levels of pro-inflammatory factors and reduced phosphorylated I?B-? and nuclear factor kappa B (NF-?B) levels in the heart. Additionally, cornuside was shown to have remarkable antioxidant activity and inhibited ISO-induced myocardial cell necrosis. Thus, cornuside appeared to protect the rat from myocardial I/R injury by acting as an anti-inflammatory agent. These findings suggested that cornuside may be used therapeutically in the setting of myocardial I/R where inflammation and oxidant injury are prominent.
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[Expression and activity of glycosylphosphatidylinositol-specific phospholipase d mRNA in bone marrow mononuclear cells isolated from patient with acute myeloid leukemia and their significance].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 02-09-2010
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This study was purposed to investigate the expression and significance of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) in bone marrow mononuclear cells (BMMNC) isolated from patients with acute myeloid leukemia (AML), GPI-PLD activity in BMMNC isolated from 78 patients with AML and 15 normal persons was measured by using GPI-anchored placental alkaline phosphatase (PLAP) as a substrate and Triton X-114 phase partitioning. The GPI-PLD mRNA expression was measured by semi-quantitive reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the mRNA expression level and activity of GPI-PLD in BMMNC from de novo AML patients were 1.86 +/- 0.32 and 46.96 +/- 7.15% respectively; the mRNA expression level and activity of GPI-PLD in BMMNC from completely remission and refractory or relapsed patients were 1.26 +/- 0.29, 33.36 +/- 5.13%and 1.79 +/- 0.19, 44.31 +/- 7.22%, while those in BMMNC from normal controls were 1.27 +/- 0.23, 35.38 +/- 5.15% respectively. The mRNA expression level and activity of GPI-PLD in de novo and refractory or relapsed patients were obviously higher than those in normal controls with significant difference (p < 0.01), while the comparison between remitted patients and normal controls showed no statistical difference (p > 0.05). It is concluded that the expression level of GPI-PLD mRNA coincides with GPI-PLD activity. The mRNA expression and activity of GPI-PLD in de novo and refractory or relapsed patients are obviously higher than those in normal controls. It is worthy of further exploring whether GPI-PLD plays a certain role in process of leukemia pathogenesis.
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Effects of maternal treatment of dehydroepiandrosterone (DHEA) on serum lipid profile and hepatic lipid metabolism-related gene expression in embryonic chickens.
Comp. Biochem. Physiol. B, Biochem. Mol. Biol.
PUBLISHED: 01-10-2010
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Over the last decade, much evidence emerged to suggest that alterations in maternal diets during pregnancy may irreversibly affect aspects of physiological and biochemical functions in the fetus. To explore the effects of maternal dietary treatments with dehydroepiandrosterone (DHEA) on lipid metabolism in the embryo, we investigated serum lipid profile and hepatic lipid metabolism-related gene expression in the maternal and embryonic chicken. Sixteen-week-old pullets were allocated into 3 groups (n=30), and after laying, they were provided with a commercial diet supplemented with DHEA at 0, 20 or 100mg/kg diet. Eggs were collected after DHEA treatment and incubated at 37.5 degrees C and a relative humidity of 60%. Blood and liver samples were collected from hens and embryonic chickens. DHEA treatment resulted in decreased body weight and increased relative liver weight in both maternal and embryonic chickens, while the concentrations of blood triglyceride (TG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C) and non-esterified fatty acid (NEFA) were significantly lower in the 20mg DHEA/kg group as compared to the control group during embryonic development. The expression of acetyl CoA carboxylase (ACC) and carnitine palmitoyl transferase I (CPTI) gene was also reduced following treatment with 20mg DHEA/kg at hatching. However, blood TC, and hepatic fatty acid synthase (FAS) and hydroxy methylglutaryl-CoA reductase (HMGR) gene expression were significantly up-regulated in the 100mg DHEA/kg group during embryonic development and hatching. Overall, the results of this study indicate that maternal dietary treatment with DHEA regulates serum lipid metabolism and hepatic gene expression.
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[Effects of artificial seawater with different N/P ratio on early development of Laminaria japonica embryo spore].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 12-02-2009
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The embryo spores of Laminaria japonica were cultured in artificial seawater with different N/P ratio to observe their early development. 80 percent of the spores cultivated in whole-ingredient artificial seawater developed normally, but the time of spore germination and gametophyte formation was lagged significantly and the amount of female gametes was significantly higher than that of male gametes (P<0.001), as compared to the control cultivated in natural seawater. Artificial seawater with different N/P ratio showed different effects on the spore germination rate. The artificial seawater with N/P > 15 decreased the spore germination rate, while that without N or P inhibited the spore germination significantly (P<0.001). It was concluded that L. japonica spores could develop normally in artificial seawater, but their germination rate was affected by the seawater N/P. The deficiency of N or P in artificial seawater would lag the time of gametophyte formation and affect the differentiation ratio of female-male gametes.
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[Synthesis and preliminary evaluation of antidiabetic activity of 4-(3-(4-bromophenyl)-3-oxo-1-arylpropylamino)-N-(5-methylisoxazol-3-yl)benzenesulfonamide].
Yao Xue Xue Bao
PUBLISHED: 11-01-2009
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Diabetes mellitus is a common metabolic disease with a high and growing prevalence affecting 4% of the population worldwide, the development of safe and effective therapeutic drug is the major thrust for chemists and pharmacists. To search for active antidiabetic lead compound, we designed and synthesized some novel beta-amino ketone derivatives containing sulfamethoxazole moiety directly through Mannich reaction of sulfamethoxazole, 4-bromoacetophenone and some aromatic aldehydes catalyzed by concentrated hydogen chloride or iodine in the solution of ethanol at 24-40 degrees C with convenient operation, mild reaction condition and satisfactory yield (32%-90%). Their chemical structures were characterized by 1H NMR, 13C NMR, MS and HR-MS. Biological activity tests showed that, in the range of low concentration (5-10 microg x mL(-1)), these title compounds to a certain degree possess protein tyrosine phosphatase 1B (PTP1B) inhibitory activity and a-glucosidase inhibitory activity, moreover, some could activate peroxisome proliferator-activated receptor response element (PPRE) moderately. The PPRE agonist activities of seven compounds are almost 40% of that of Pioglitazone (the positive control), compound 12 shows the strongest activity (66.35%) among them. Thus, it was found that some of 4-(3-(4-bromophenyl)-3-oxo-1-arylpropylamino)-N-(5-methyl-isoxazol-3-yl) benzenesulfonamide containing sulfamethoxazole moiety exhibited antidiabetic activity for the first time.
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[Decoloration and degradation of rhodamine B by microwave-promoted Fenton-like reaction].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-21-2009
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Microwave-promoted Fenton-like reaction, the combination of Fenton-like reagent with microwave, is an efficient method for waste water treatment. In the present paper, the degradation of rhodamine B (a kind of organic dye) using this method was studied. Through numerous experiments, the influences of various parameters including the initial pH value, reaction time, dosage of K2Cr2O7, dosage of H2O2 and microwave were investigated intensively. The characteristic curve of rhodamine B, the concentration-absorbency curve of rhodamine B, the orthogonal optimization tests and comparative tests were given. The mechanism of this reaction was also probed. It is concluded from the experiments that the microwave can accelerate the process of degradation effectively. Under optimal conditions, the overall color removal was more than 99.9% within 9 min. In the study, the method for characterization was entirely UV-Vis spectral analysis.
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[Development of new SSR markers from EST of SSH cDNA libraries on rose fragrance].
Yi Chuan
PUBLISHED: 10-13-2009
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The new SSR markers of rose related fragrance were developed based on the SSH cDNA libraries of rose floral scent mutant. In this study, 10 EST-SSRs (2.6%) from 391 ESTs in the libraries were identified. Six EST-SSRs primers were designed to sequence flanking SSRs. The primer pairs designed were screened on the wild-type Jinyindao, which has flowers full of pleasant scent, and the mutant-type Wangriqinghuai without perceivable floral scent. Five primer pairs were amplified effectively in Jinyindao and Wangriqinghuai, and 3 were polymorphic between Jinyindao and Wangriqinghuai. Eighteen rose cultivars including fragrant roses and nonfragrant roses were identified by the five prime pairs. These results proved that EST-SSR markers are effective markers to identify the polymorphism of the rose.
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Use of comparative proteomics to identify key proteins related to hepatic lipid metabolism in broiler chickens: evidence accounting for differential fat deposition between strains.
Lipids
PUBLISHED: 08-07-2009
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In order to investigate differences in fat metabolism during embryonic development, a comparative proteomics strategy was employed using Arbor Acres (AA) and San Huang (SH) broiler chickens with different growth and fat deposition characteristics. These birds were floor-reared and fed identical diets, and embryonic livers were collected from AA and SH chicken embryos on days 9, 14 and 19 of incubation and hatching. Proteins were extracted and fractionated by two-dimensional electrophoresis (2-DE), Neuhoffs colloidal Coomassie Blue G-250 staining was carried out, and stained gels were scanned and analyzed using PDQuest7.3 software (Bio-Rad). In-gel trypsin digestion of the differential protein spots and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) were subsequently assessed. Peptide mass fingerprinting of the differentially expressed proteins was performed using the server from MASCOT or either Prospector or ProFound, and 37 proteins were successfully identified. In the present study, embryo and liver weights showed a trend toward enhanced growth during embryonic development. Of the 37 identified differential proteins, phosphoenolpyruvate carboxykinase (PEPCK), apolipoprotein A-I (Apo A-I), fatty acid-binding protein (L-FABP) and 3-hydroxy-3-methylglutaryl-Coenzyme A synthase (HMG-CoA synthase) were up-regulated in SH chickens to a greater extent than they were in AA chickens. These observations suggest that the lipid metabolic proteins and enzymes are inherent characteristics that contribute to the apparent differences in fat deposition between the two strains.
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The role of Crk/Dock180/Rac1 pathway in the malignant behavior of human ovarian cancer cell SKOV3.
Tumour Biol.
PUBLISHED: 08-05-2009
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Small GTPases, particularly the Rho family, are key regulators of cell motility and migration. Dock180 was well known for the main target of signal adaptor protein Crk and acted as a guanine-nucleotide exchange factor for small GTPase Rac1. In the present study, Dock180 was found to combine primarily with CrkI other than CrkII, and its association with Elmo1 was also demonstrated in ovarian cancer cell SKOV3. To evaluate the role of Dock180 in human ovarian cancer cell, we performed RNAi-mediated knockdown of Dock180 in SKOV3 cells using small interfering RNA expression vector. In Dock180 knockdown cells, we found that Elmo1 expression and Rac1 activity were decreased simultaneously. By contrast, the expressions of both another Crk-combining molecule C3G and Rap1 activity were observed to increase obviously. Accordingly, all Dock180 knockdown cells present with evident change in cell morphology, reduced cell proliferation, and attenuated cell migration. Taken together, these results suggest that signal transfer of Crk/Dock180/Rac1 is implicated in actin cytoskeleton reorganization and thus in the cell proliferation, motility, invasion, and of human ovarian cancer cell line SKOV3.
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Asiatic acid induces colon cancer cell growth inhibition and apoptosis through mitochondrial death cascade.
Biol. Pharm. Bull.
PUBLISHED: 08-05-2009
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Cancer is one of the leading causes of death in the world. The triterpenoid compound asiatic acid derived from the tropical medicinal plant Centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. The present work studies asiatic acid-mediated growth inhibition of cancer cells and the underlying mechanism. Asiatic acid markedly inhibited cancer cell proliferation. Apoptosis of SW480 human colon cancer cells was induced by asiatic acid as shown by flow cytometry, DNA fragmentation and nuclear chromatin condensation experiments. Through increasing mitochondrial membrane permeability and cytochrome c release from mitochondria into cytosol, asiatic acid induced caspase-9 activity, which further activated caspase-3 and poly(ADP-ribose) polymerase cleavage resulting in irreversible apoptotic death in the tumor cells. Taken together, these results suggest that mitochondrial death apoptosis cascade plays very important roles in asiatic acid-induced cancer apoptosis.
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The expression of serum steroid sex hormones and steroidogenic enzymes following intraperitoneal administration of dehydroepiandrosterone (DHEA) in male rats.
Steroids
PUBLISHED: 07-28-2009
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The adrenals of humans and primates could secrete large amounts of dehydroepiandrosterone (DHEA) and its sulphate ester (DHEA-S) in the circulation, which act as precursors of active steroid hormones in a long series of peripheral target intracrine tissues. The marked decline of serum DHEA and DHEA-S concentrations with age in humans has been incriminated in the development of various pathologies. Therefore, this study aims to provide detailed information on the effects of the intraperitoneal injection of DHEA on circulating steroid hormones and their metabolites and their trade-off relationship over 24 h in male rats. In this study, 100 healthy adult male Sprague-Dawley (SD) rats were randomly divided into three groups: control, 25 mg kg(-1) DHEA-treated and 100 mg kg(-1) DHEA-treated. The animals were sacrificed at 0, 1.5, 3, 6, 12 or 24 h, and the samples were collected for subsequent analysis. Total cholesterol (TC) markedly decreased 3h after the administration of 100 mg kg(-1) DHEA, but markedly increased 12h after administration. The DHEA-S, progesterone (P), testosterone (T), oestradiol (E(2)), cortisol (Cor) and aldosterone (Ald) concentrations also markedly increased after DHEA administration, with serum DHEA-S, T, E(2) and Cor levels peaking at 1.5 h. Over time, steroid hormone levels were depressed, but serum Cor and Ald levels were markedly elevated relative to the control group at 24 h. Furthermore, DHEA treatment produced a significant increase in P450scc, 17beta-HSDIII, CYP17alpha and 3beta-HSD mRNA expression at 1.5 h, but a decided decrease in P450scc and StAR mRNA expression at 12 and 24 h, and CYP17alpha and 17beta-HSDIII expression at 12 h in the 100 mg kg(-1) DHEA group. In total, the results of the present study indicate that DHEA at high pharmacological doses may affect steroid through an effect on steroidogenic enzymes.
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[Expressions of CXCL13, CD10 and bcl-6 in angioimmunoblastic T-cell lymphoma and peripheral T-cell lymphoma, not otherwise specified].
Zhonghua Bing Li Xue Za Zhi
PUBLISHED: 07-07-2009
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To study the value of immunomarkers CXCL13, CD10, bcl-6 in pathologic diagnosis of angioimmunoblastic T-cell lymphoma (AITL).
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Suppression of tumor necrosis factor receptor associated factor (TRAF)-2 attenuates the proinflammatory and proliferative effect of aggregated IgG on rat renal mesangial cells.
Cytokine
PUBLISHED: 05-31-2009
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Immune-complex (IC) mediated glomerulonephritis (GN) is a common cause of chronic kidney disease associated with increased levels of tumor necrosis factor (TNF)-alpha in renal cells. TNF-alpha signaling pathways involve complicated interactions between multiple proteins including TNF-receptor-associated factor (TRAF)-2. We have previously found markedly up-regulated expression of TRAF-2 in renal tissues from IC mediated lupus nephritis patients. Here we investigated the effect of TRAF-2 on inflammatory response in rat mesangial cells (MCs). The results showed that treatment with soluble aggregated IgG (AIgG) resulted in a time- and dose-dependent increase in the expression of interleukin (IL)-1beta and IL-6. Significant cell proliferation was also observed after the treatment with soluble AIgG. Knockdown TRAF-2 by siRNA significantly suppressed soluble AIgG induced up-regulation of TRAF-2, IL-1beta, and IL-6. Meanwhile the cell proliferation was inhibited and apoptotic cells were increased. It was concluded that TRAF-2 could induce the proinflammatory and proliferative effects of soluble AIgG on rat MCs. Thus, TRAF-2 may represent a future target for therapy of IC mediated GN.
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[Study on the hydrolysis distribution of ferric saline by infrared spectrophotometry and single crystal X-ray diffraction method].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 05-19-2009
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The hydrolytic stability of Fe(a), Fe(b) and Fe(c) in different pH values of poly-ferric-flocculants was studied by using Fe-ferron time by time complexation colorimetry. The research results showed that Fe(b) was unstable, and all Fe(b) was transformed to Fe(c) after 10-15 d placement. The content of Fe(c) tended towards stability after 10-15 d. Also, the content of Fe(a) tended towards stability after 10 d. The single crystal was synthesized by the method of direct crystallization in Fe(III)-SO4(2-) water solution at normal temperature and its structure characteristic was studied by single crystal X ray diffraction method and IR (infrared spectrophotometry). The research results showed that there was no group of Fe-OH-Fe, Fe-OH and binary ferric complexed with two hydroxyl groups in the single crystal synthesized from the ferric aqueous solution in low pH (pH was about 0.5). The form of Fe in single crystal was all Fe(III). The chemical formula of the single crystal was Fe(H2O)6 (SO4)2NH4 x 6H2O when the ammonia water was used as the alkalinizing agent. One reason was that with the evaporation of water, these single crystals were synthesized at pH 0. 5 despite of different initial pH and different initial alkalinizing agents. Another reason was that the hydrolysis distribution of ferric saline was unstable. Therefore, it was not easy to obtain the single crystal of Fe(III)-hydroxy complexes or Fe(III)-polymer at low pH value. The study showed that infrared spectrophotometry and single crystal X ray diffraction method have a good prospect in the research on hydrolysis distribution of flocculants.
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