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Find video protocols related to scientific articles indexed in Pubmed.
[Studies on the correlation between the expression of Toll-like receptor 4 and the synovitis of the temporomandibular joint in rats].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 10-30-2014
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To investigate the expression of the Toll-like receptor-4 (TLR-4) in temporo-mandibular joint synovitis in rats, and to discuss the correlation between the expression of TLR-4 and the synovitis.
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CXCR4 Signaling Induced Epithelial-Mesenchymal Transition by PI3K/AKT and ERK Pathways in Glioblastoma.
Mol. Neurobiol.
PUBLISHED: 09-15-2014
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Stromal cell-derived factor 1 (SDF-1) and its receptor, CXCR4, play an important role in tumor progression. Epithelial-mesenchymal transition (EMT) process is linked to disease pathophysiology. This study aimed to investigate the roles and underlying mechanisms of SDF-1/CXCR4 axis in EMT process of glioblastoma. In the present study, CXCR4 activation and inhibition in U87 were induced with exogenous SDF-1 and with CXCR4 small interfering RNA (siRNA), respectively. CXCR4 downstream signal molecules AKT, ERK, and EMT biomarkers (vementin, snail, N-cadherin, and E-cadherin) were tested using the Western blot. Our results showed that SDF-1 can induce AKT and ERK phosphorylation in a dose-dependent manner, and endogenous CXCR4 can be blocked thoroughly by CXCR4 siRNA in U87. Notably SDF-1 alone treatment can induce the upregulation of vementin, snail, and N-cadherin of U87; besides, the downregulation of E-cadherin also occurred. On the contrary, CXCR4 siRNA significantly prohibited SDF-1-induced AKT and ERK phosphorylation, at the same time, EMT biomarker changes were not observed. Function analysis revealed that CXCR4 siRNA obviously interfered with U87 cell migration and proliferation, according to wound healing assay. In conclusion, this study suggested that EMT process can be triggered by the SDF-1/CXCR4 axis in glioblastoma, and then involved in the tumor cell invasion and proliferation via activation of PI3K/AKT and ERK pathway. Our study lays a new foundation for the treatment of glioblastoma through antagonizing CXCR4.
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Facile immobilization of ag nanocluster on nanofibrous membrane for oil/water separation.
ACS Appl Mater Interfaces
PUBLISHED: 08-20-2014
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Superhydrophobic and superoleophilic electrospun nanofibrous membranes exhibiting excellent oil/water separation performance were green fabricated by a facile route combining the amination of electrospun polyacrylonitrile (APAN) nanofibers and immobilization of a Ag nanocluster with an electroless plating technique, followed by n-hexadecyl mercaptan (RSH) surface modification. By introducing the hierarchically rough structures and low surface energy, the pristine superhydrophilic APAN nanofibrous membranes could be endowed with a superhydrophobicity with water contact angle of 171.1 ± 2.3°, a superoleophilicity with oil contact angle of 0° and a self-cleaning surface arising from the extremely low water contact angle hysteresis (3.0 ± 0.6°) and a low water-adhesion property. Surface morphology studies have indicated that the selective wettability of the resultant membranes could be manipulated by tuning the electroless plating time as well as the hierarchical structures. More importantly, the extremely high liquid entry pressure of water (LEPw, 175 ± 3 kPa) and the robust fiber morphology of the APAN immobilized Ag nanocluster endowed the as-prepared membranes with excellent separation capability and stability for oil/water separation by a solely gravity-driven process. The resultant membranes exhibited remarkable separation efficiency in both hyper-saline environment and broad pH range conditions, as well as excellent recyclability, which would make them a promising candidate for industrial oil-contaminated water treatments and marine spilt oil cleanup, and provided a new prospect to achieve functional nanofibrous membranes for oil/water separation.
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Cellular immune response in patients with chronic hepatitis B virus infection.
Microb. Pathog.
PUBLISHED: 08-12-2014
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Hepatitis, cirrhosis and hepatocellular carcinoma caused by hepatitis B virus (HBV) infection has threatening human health seriously. As HBV is a kind of non-cytotoxic virus, host immune response plays a vital role in pathogenesis and clinical outcomes of hepatitis B. Multiple immune cells (e.g., including cytotoxic T lymphocytes, regulatory T cells, natural killer cells, dendritic cells) are important in the immune regulation of HBV infection. Therefore, focusing on the activation states of immune cells may provide new evidences and strategies for determining immune status of HBV infectors, monitoring progression of diseases and predicting efficacy of antiviral treatment.
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Compatibility of stabilized whole blood products with CD4 technologies and their suitability for quality assessment programs.
PLoS ONE
PUBLISHED: 08-08-2014
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CD4 T cell enumeration is the most widely used prognostic marker for management of HIV disease. Internal quality control and external quality assessment (EQA) programs are critical to ensure reliability of clinical measurements. The utility of stabilized whole blood products (SWBP) as a test reagent for EQA programs such as Quality Assessment and Standardization for Immunological measures relevant to HIV/AIDS (QASI) program have been demonstrated previously. Since then, several new commercial SWBPs and alternative CD4 enumeration technologies have become available. Seven SWBPs were evaluated on seven different enumeration platforms to determine which product(s) are most suitable for EQA programs that support multiple analytical technologies.
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Wogonin Reverses Multi-Drug Resistance of Human myelogenous leukemia K562/A02 cells via Downregulation of MRP1 Expression by Inhibiting Nrf2/ARE Signaling Pathway.
Biochem. Pharmacol.
PUBLISHED: 07-28-2014
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Constitutive NF-E2-related factor 2(Nrf2) activation has been recently reported to play a pivotal role in enhancing cell survival and resistance to anticancer drugs in many tumors. Previously, much effort has been devoted to the investigation of blocking Nrf2 function in cultured cells and cancer tissues, but few research has been undertaken to evaluate the precise mechanism of flavonoids-induced sensitivity by inhibiting Nrf2. In this study, we investaged the reversal effect of Wogonin, a flavonoid isolated from the root of Scutellaria baicalensis Georgi, in resistant human myelogenous leukemia. Data indicated that Wogonin had strong reversal potency by inhibiting functional activity and expression of MRP1 at both protein and mRNA in adriamycin(ADR)-induced resistant human myelogenous leukemia K562/A02 cells. Consequently, the inhibition of MRP1 by Wogonin was dependent on Nrf2 through the decreased binding ability of Nrf2 to antioxidant response element(ARE). Further research revealed Wogonin modulated Nrf2 through the reduction of Nrf2mRNA at transcriptional processes rather than RNA degradation, which is regulated by the PI3K/Akt pathway. Moreover, DNA-PKcs was found to be involved in the Wogonin-induced downregulation of Nrf2 mRNA at transcriptional levels. In summary, these results clearly demonstrated the effectiveness of using Wogonin via inhibiting Nrf2 to combat chemoresistance and suggested that Wogonin can be developed into an efficient natural sensitizer for resistant human myelogenous leukemia.
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Therapeutic value of transcatheter arterial chemoembolization combined with portal vein embolization for primary hepatocellular carcinoma with portal vein tumor thrombus: A pilot study.
Asia Pac J Clin Oncol
PUBLISHED: 07-20-2014
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To compare clinical outcome and safety of transcatheter arterial chemoembolization (TACE)?+?portal vein embolization (PVE) with TACE alone in hepatocellular carcinoma (HCC) patients with portal vein tumor thrombus (PVTT).
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Complete mitochondrial genome of Macropodus ocellatus (Perciformes: Anabantidae: Macropodusinae).
Mitochondrial DNA
PUBLISHED: 07-02-2014
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Abstract In this study, we cloned and sequenced the complete mitochondrial genome of Macropodus ocellatus. The genome is a circular molecule of 16,501?bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light strand replication). The gene composition and order of which were similar to most other vertebrates. All protein-coding genes are initiated with ATG except for COX 1, which begin with GTG instead. However, the termination codons of 13 protein-coding genes are varied with TAA, TA, T or AGG. This mitogenome sequenc data would contribute to better understanding species identification, evolutionary relationships and population genetics of the Macropodus.
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Intakes of fat and micronutrients between ages 13 and 18 years and the incidence of proliferative benign breast disease.
Cancer Causes Control
PUBLISHED: 07-01-2014
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Dietary exposures during adolescence may exert important effects on breast development and future breast cancer risk. This study evaluated the associations between high school intakes of fat and micronutrients and the incidence of proliferative benign breast disease (BBD), a marker of increased breast cancer risk.
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[Effects of different tooth preparations on the fracture behavior of teeth with severe wedge-shaped defect restored with post and core crowns].
Hua Xi Kou Qiang Yi Xue Za Zhi
PUBLISHED: 06-03-2014
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This study aimed to investigate the effects of different tooth preparations on the fracture strength and pattern of failure of teeth with severe wedge-shaped defect restored with post and core crowns.
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NGF and proNGF simultaneously promote symmetric self-renewal, quiescence and EMT to enlarge the breast cancer stem cell compartment.
Stem Cells
PUBLISHED: 04-16-2014
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The discovery of cancer stem cells (CSCs) fundamentally advanced our understanding of the mechanisms governing breast cancer development. However, the stimuli that control breast CSC self-renewal and differentiation have still not been fully detailed. We previously showed that nerve growth factor (NGF) and its precursor proNGF can stimulate breast cancer cell growth and invasion in an autocrine manner. In the present study, we investigated the effects of NGF and proNGF on the breast CSC compartment and found that NGF or proNGF enrich for CSCs in several breast cancer cell lines. This enrichment appeared to be achieved by increasing the number of symmetric divisions of quiescent/slow-proliferating CSCs. Interestingly, in vitro NGF pretreatment of MCF-7 luminal breast cancer cells promoted epithelial to mesenchymal transition (EMT) in tumors of SCID mice. Furthermore, p75(NTR) , the common receptor for both neurotrophins and proneurotrophins, mediated breast CSC self-renewal by regulating the expression of pluripotency transcription factors. Our data indicate, for the first time, that the NGF/proNGF/p75(NTR) axis plays a critical role in regulating breast CSC self-renewal and plasticity. Stem Cells 2014.
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Complete mitochondrial genome of the Chinese bitterling Acheilognathus macropterus (Cypriniformes: cyprinidae).
Mitochondrial DNA
PUBLISHED: 04-15-2014
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Abstract Acheilognathus macropterus belongs to the family Acheilognathinae. In the present study, we obtain the complete mitochondrial genome of the Chinese bitterling A. macropterus by PCR amplification and DNA sequencing. It is a circular double-stranded DNA molecule of 16,773?bp in length, containing 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and two main non-coding regions (the control region and the origin of the light strand replication). The gene composition and order of which are similar to most other vertebrates. Compared with Acanthorhodeus macropterus, they share 98.90% nucleotide sequences similarity, and the biggest nucleotide sequence discrepancy between homologous genes are observed in ND5 gene. The molecular data we presented here could provide useful information for the studies on species identification, evolutionary relationships and population genetics of the Acheilognathinae.
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CMTM5 exhibits tumor suppressor activity through promoter methylation in oral squamous cell carcinoma.
Biochem. Biophys. Res. Commun.
PUBLISHED: 03-27-2014
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Oral squamous cell carcinoma (OSCC) is one of the most common types of malignancies in the head and neck region. CKLF-like MARVEL transmembrane domain-containing member 5 (CMTM5) has been recently implicated as a tumor suppressor gene in several cancer types. Herein, we examined the expression and function of CMTM5 in oral squamous cell carcinoma. CMTM5 was down-regulated in oral squamous cell lines and tumor samples from patients with promoter methylation. Treatment with the demethylating agent 5-aza-2'-deoxycytidine restored CMTM5 expression. In the OSCC cell lines CAL27 and GNM, the ectopic expression of CMTM5-v1 strongly inhibited cell proliferation and migration and induced apoptosis. In addition, CMTM5-v1 inhibited tumor formation in vivo. Therefore, CMTM5 might act as a putative tumor suppressor gene through promoter methylation in oral squamous cell carcinoma.
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Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay.
BMC Infect. Dis.
PUBLISHED: 03-18-2014
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A novel avian influenza A (H7N9) virus emerged in eastern China in February 2013. 413 confirmed human cases, including 157 deaths, have been recorded as of July 31, 2014.
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Changes of costimulatory molecule CD28 on circulating CD8+ T cells correlate with disease pathogenesis of chronic hepatitis B.
Biomed Res Int
PUBLISHED: 02-27-2014
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Costimulatory signals are critical for antiviral immunity. The aim of this study was to evaluate the change of costimulatory molecule CD28 on circulating CD8+ T cells in chronic hepatitis B patients (CHB). Seventy CHB patients and fifty-six healthy controls were included, and forty-eight CHB patients were recruited for 52 weeks of longitudinal investigation. The proportions of circulating CD8+CD28+ and CD8+CD28- subpopulations were determined by flow cytometry, and the CD8+CD28+/CD8+CD28- T cells ratio was calculated. Compared with the subpopulation in healthy controls, high proportions of CD8+CD28- subpopulation were observed in CHB patients. Similarly, the CD8+CD28+/CD8+CD28- T cells ratio was significantly decreased in CHB patients compared with healthy controls and correlated significantly with hepatitis B virus (HBV) loads. High proportions of CD8+CD28- subpopulation and low CD8+CD28+/CD8+CD28- T cells ratio were observed in hepatitis B e antigen- (HBeAg-) positive individuals as compared with that in HBeAg-negative subjects. A significant decrease in CD8+CD28- subpopulation, increase in CD8+CD28+ subpopulation, and CD8+CD28+/CD8+CD28- T cells ratio were seen in those patients who received efficient antiviral therapy. Thus, aberrant CD28 expression on circulating CD8+ T cells and the CD8+CD28+/CD8+CD28- T cells ratio reflect the dysregulation of T cell activation and are related to the pathogenesis of chronic HBV infection.
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Dynamic behavior of lymphocyte subgroups correlates with clinical outcomes in human H7N9 infection.
J. Infect.
PUBLISHED: 02-17-2014
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To investigate peripheral blood lymphocyte subgroups response to the H7N9 virus and identify potential correlations between the anti-viral response and clinical outcomes in infected patients.
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Dual-biomimetic superhydrophobic electrospun polystyrene nanofibrous membranes for membrane distillation.
ACS Appl Mater Interfaces
PUBLISHED: 02-05-2014
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A new type of dual-biomimetic hierarchically rough polystyrene (PS) superhydrophobic micro/nano-fibrous membrane was fabricated via a one-step electrospinning technique at various polymer concentrations from 15 to 30 wt %. The obtained micro/nano-fibers exhibited a nanopapillose, nanoporous, and microgrooved surface morphology that originated from mimicking the micro/nanoscale hierarchical structures of lotus leaf and silver ragwort leaf, respectively. Superhydrophobicity and high porosity of such resultant electrospun nanofibrous membranes make them attractive candidates for membrane distillation (MD) application with low energy water recovery. In this study, two kinds of optimized PS nanofibrous membranes with different thicknesses were applied for desalination via direct contact MD. The membranes maintained a high and stable permeate water vapor flux (104.8 ± 4.9 kg/m(2)·h, 20 g/L NaCl salt feed for a thinner PS nanofibrous membrane with thickness of 60 ?m; 51 ± 4.5 kg/m(2)·h, 35 g/L NaCl salt feed for the thicker sample with thickness of 120 ?m; ?T = 50 °C) for a test period of 10 h without remarkable membrane pores wetting detected. These results were better than those of typical commercial polyvinylidene fluoride (PVDF) MD membranes or related PVDF nanofibrous membranes reported in literature, suggesting excellent competency of PS nanofibrous membranes for MD applications.
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Growth charts for Chinese Down syndrome children from birth to 14 years.
Arch. Dis. Child.
PUBLISHED: 01-30-2014
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To establish Down syndrome (DS)-specific growth charts for Hong Kong Chinese children.
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Role of p75 neurotrophin receptor in stem cell biology: more than just a marker.
Cell. Mol. Life Sci.
PUBLISHED: 01-14-2014
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p75(NTR), the common receptor for both neurotrophins and proneurotrophins, has been widely studied because of its role in many tissues, including the nervous system. More recently, a close relationship between p75(NTR) expression and pluripotency has been described. p75(NTR) was shown to be expressed in various types of stem cells and has been used to prospectively isolate stem cells with different degrees of potency. Here, we give an overview of the current knowledge on p75(NTR) in stem cells, ranging from embryonic to adult stem cells, and cancer stem cells. In an attempt to address its potential role in the control of stem cell biology, the molecular mechanisms underlying p75(NTR) signaling in different models are also highlighted. p75(NTR)-mediated functions include survival, apoptosis, migration, and differentiation, and depend on cell type, (pro)neurotrophin binding, interacting transmembrane co-receptors expression, intracellular adaptor molecule availability, and post-translational modifications, such as regulated proteolytic processing. It is therefore conceivable that p75(NTR) can modulate cell-fate decisions through its highly ramified signaling pathways. Thus, elucidating the potential implications of p75(NTR) activity as well as the underlying molecular mechanisms of p75(NTR) will shed new light on the biology of both normal and cancer stem cells.
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Complete mitochondrial genome of Macrognathus aculeatus (Synbranchiformes: Mastacembelidae).
Mitochondrial DNA
PUBLISHED: 01-14-2014
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Abstract In this study, we cloned and sequenced the complete mitochondrial genome (mitogenome) of Macrognathus aculeatus. The genome is a circular molecule of 16,537?bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light strand replication). The overall base composition of M. aculeatus is 30.03% for A, 26.46% for T, 28.72% for C and 14.79% for G, with a slight A?+?T bias of 56.49%. It has the typical vertebrate mitochondrial gene arrangement.
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A highly selective fluorescent probe for Cu2+ based on rhodamine B derivative.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 01-10-2014
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A new fluorescent probe 1 for Cu(2+) based on a rhodamine B derivative was designed and synthesized. Probe 1 displays high sensitivity toward Cu(2+) and about a 37-fold increase in fluorescence emission intensity is observed upon the addition of 10 equiv. Cu(2+) in 50% water/ethanol buffered at pH 7.10. Besides, upon binding Cu(2+) a remarkable color change from colorless to pink was easily observed by the naked eyes. The reversible dual chromo- and fluorogenic response toward Cu(2+) is likely due to the chelation-induced ring-opening of rhodamine spirolactam. The linear response range covers a concentration range of Cu(2+) from 8.0×10(-7) to 1.0×10(-4) mol/L and the detection limit is 3.0×10(-7) mol/L. Except Co(2+), the probe exhibits high selectivity for Cu(2+) over a large number of cations such as alkaline, alkaline earth and transitional metal ions. The accuracy and precision of the method were evaluated by the analysis of the standard reference material, copper in water (1.0 mol/L HNO3). The proposed probe has been used for direct measurement of Cu(2+) content in river water samples and imaging of Cu(2+) in living cells with satisfying results, which further demonstrates its value of practical applications in environmental and biological systems.
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Short communication: interaction of bovine milk protein with chlorpyrifos.
J. Dairy Sci.
PUBLISHED: 01-01-2014
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Dairy products are considered as nutrient-dense foods and consumed by many people in western countries, as well as an increasing number of Asian people. Excessive and frequent application of pesticides on vegetables and fruits leads to a potential health hazard to consumers. The organophosphate insecticide chlorpyrifos has been reported to bind with human and bovine serum albumin. Thus, it is necessary to explore the interaction between food protein and chlorpyrifos. In this study, equilibrium dialysis and fluorescence spectra were used to demonstrate binding of milk proteins to chlorpyrifos. The amount of milk protein bound was 0.03±0.01mg/g. Moreover, the milk protein-chlorpyrifos complexes were stable at pH 3.5to 9.5 and ion concentrations from 0.1 to 1.0M. The amount of chlorpyrifos bound to milk proteins decreased to 50% after being in vitro digested by pepsin and trypsin. The results showed that the interaction between food proteins and the pesticide might partially remove the insecticide and reduce the concentration of pesticide absorbed into the blood and, thus, alleviate the corresponding toxicity.
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Complete mitochondrial genome of the Chinese bitterling Rhodeus sinensis (Cypriniformes: Cyprinidae).
Mitochondrial DNA
PUBLISHED: 10-05-2013
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Abstract The mitochondrial genome of the Chinese bitterling Rhodeus sinesis is a circular molecule of 16,677?bp in length, containing 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and two main non-coding regions (the control region and the origin of the light strand replication). The gene composition and order of which were similar to most other vertebrates. All protein-coding genes are initiated with ATG except for COX 1, which begin with GTG instead. However, the termination codons of 13 protein-coding genes are varied with TAA, TA, T or TAG. The molecular data we presented here could provide useful information for the studies on species identification, evolutionary relationships and population genetics of the Acheilognathinae.
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Combined use of the BinaxNOW Staphylococcus aureus test with the Clearview PBP2a assay for the early detection of methicillin-resistant S. aureus from positive blood cultures.
Diagn. Microbiol. Infect. Dis.
PUBLISHED: 09-20-2013
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The use of combination 2 novel immunochromatographic assays for same-day detection of methicillin-resistant Staphylococcus aureus (MRSA) from positive blood cultures was evaluated. Compared to the standard culture methods, the BinaxNOW® S. aureus test demonstrated 98.7% sensitivity and 100% specificity in correctly identifying S. aureus. The sensitivity and specificity of the Clearview® PBP2a assay in differentiating MRSA from methicillin-susceptible S. aureus were 97.1% and 100%.
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Organization of the mitochondrial genome of Odontobutis sinensis (Perciformes: Odontobutidae): rearrangement of tRNAs and additional non-coding regions.
Mitochondrial DNA
PUBLISHED: 09-20-2013
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Abstract In this study, we cloned and sequenced the complete mitochondrial genome of Odontobutis sinensis. The genome was found to be 17,441?bp in size with a mostly conserved structural organization when compared with that of other teleost fish. It contained 37 genes (13 protein-coding genes, 22 transfer RNA genes and two ribosomal RNA genes) and two main non-coding regions (the control region and the origin of the light strand replication). Rearrangements of tRNAs (tRNA-Ser, tRNA-Leu, tRNA-His) and three additional longer non-coding regions (51?bp, 622?bp and 66?bp, respectively) were present between the ND4 and ND5 genes. Within the control region, one 322?bp long tandem repeat area (7*46?bp) and some typical conserved domains were identified. The molecular data here we presented could play a useful role to study the evolutionary relationships and population genetics of the Odontobutidae.
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Complete mitochondrial genome of the freshwater goby Odontobutis potamophila (Perciformes: Odontobutidae).
Mitochondrial DNA
PUBLISHED: 09-20-2013
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Abstract In this study, we cloned and sequenced the complete mitochondrial genome of Odontobutis potamophila. The genome was found to be 16,932?bp in size with a mostly conserved structural organization when compared with that of other teleost fish. It contained 37 genes (13 protein-coding genes, 22 transfer RNA genes, and 2 ribosomal RNA genes) and 2 main non-coding regions (the control region and the origin of the light strand replication). Rearrangements of tRNAs (tRNA-Ser, tRNA-Leu, tRNA-His) and three additional longer non-coding regions (43?bp, 336?bp and 76?bp, respectively) were present between the ND4 and ND5 genes. Within the control region, typical conserved domains, such as the termination-associated sequence, central and conserved sequence blocks domains were identified. This mitogenome sequence data would contribute to better understanding population genetics and phylogenetic analysis of the Odontobutidae.
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Complete mitochondrial genome of Rhinogobius giurinus (Perciformes: Gobiidae: Gobionellinae).
Mitochondrial DNA
PUBLISHED: 09-20-2013
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Abstract In this study, we present the complete mitochondrial gene for the freshwater goby Rhinogobius giurinus. The genome is a circular molecule of 16,520?bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light strand replication). The overall base composition of R. giurinus is 26.08% for T, 29.56% for C, 27.73% for A and 16.63% for G, with a slight A?+?T bias of 53.81%. It has the typical vertebrate mitochondrial gene arrangement.
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Whole transcriptome sequencing reveals genes involved in plastid/chloroplast division and development are regulated by the HP1/DDB1 at an early stage of tomato fruit development.
Planta
PUBLISHED: 07-24-2013
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The phenotype of tomato high pigment-1 (hp1) mutant is characterized by overproduction of pigments including chlorophyll and carotenoids during fruit development and ripening. Although the increased plastid compartment size has been thought to largely attribute to the enhanced pigmentation, the molecular aspects of how the HP1/DDB1 gene manipulates plastid biogenesis and development are largely unknown. In the present study, we compared transcriptome profiles of immature fruit pericarp tissue between tomato cv. Ailsa Craig (WT) and its isogenic hp1 mutant. Over 20 million sequence reads, representing > 1.6 Gb sequence data per sample, were generated and assembled into 21,972 and 22,167 gene models in WT and hp1, respectively, accounting for over 60 % official gene models in both samples. Subsequent analyses revealed that 8,322 and 7,989 alternative splicing events, 8833 or 8510 extended 5-UTRs, 8,263 or 8,939 extended 3-UTRs, and 1,136 and 1,133 novel transcripts, exist in WT and hp1, respectively. Significant differences in expression level of 880 genes were detected between the WT and hp1, many of which are involved in signaling transduction, transcription regulation and biotic and abiotic stresses response. Distinctly, RNA-seq datasets, quantitative RT-PCR analyses demonstrate that, in hp1 mutant pericarp tissue at early developmental stage, an apparent expression alteration was found in several regulators directly involved in plastid division and development. These results provide a useful reference for a more accurate and more detailed characterization of the molecular process in the development and pigmentation of tomato fruits.
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The complete mitochondrial genome of Micropercops swinhonis (Perciformes: Gobioidei: Odontobutidae).
Mitochondrial DNA
PUBLISHED: 07-03-2013
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Abstract For understanding the phylogenetic position of Micropercops swinhonis within the family Odontobutidae, the complete nucleotide sequence of M. swinhonis mitochondrial genome was firstly determined. The genome is 16,493?bp in length, and consists of 37 genes (13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes) and 2 main noncoding regions (the control region and the origin of the light strand replication). The gene composition and order of which were similar to most other vertebrates. Within the control region, typical conserved domains, such as the termination-associated sequence, central and conserved sequence blocks domains were identified.
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[Variation of sperm DNA fragmentation index in male partners from infertile couples].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 06-08-2013
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To investigate variation of sperm DNA fragmentation index (DFI) in male partners of infertile couples.
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Status and determinants of health behavior knowledge among the elderly in China: a community-based cross-sectional study.
BMC Public Health
PUBLISHED: 05-20-2013
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Limited studies are available on health behavior knowledge among the elderly and the interaction between the elderly and their children living with them. Using a survey of the elderly in the community and their children living with them, we explored the characteristics that determine health behavior knowledge. We also identified the influencing factors affecting such knowledge. The relationship between the health behavior knowledge of the elderly and that of their children was also investigated.
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Novel pyrrolopyridinone derivatives as anticancer inhibitors towards Cdc7: QSAR studies based on dockings by solvation score approach.
Eur J Pharm Sci
PUBLISHED: 05-19-2013
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A series of pyrrolopyridinone derivatives as specific inhibitors towards the cell division cycle 7 (Cdc7) was taken into account, and the efficacy of these compounds was analyzed by QSAR and docking approaches to gain deeper insights into the interaction mechanism and ligands selectivity for Cdc7. By regression analysis the prediction models based on Grid score and Zou-GB/SA score were found, respectively with good quality of fits (r(2)=0.748, 0.951; r(cv)(2)=0.712, 0.839). The accuracy of the models was validated by test set and the deviation of the predicted values in validation set using Zou-GB/SA score was smaller than that using Grid score, suggesting that the model based on Zou-GB/SA score provides a more effective method for predicting potencies of Cdc7 inhibitors.
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[Effect of Kruppel like factor 4 gene silencing on apoptosis and phagocytosis of murine RAW264.7 macrophages].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
PUBLISHED: 05-07-2013
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To investigate the effect of down-regulation of Kruppel like factor 4 (KLF4) by RNA interference on proliferation, apoptosis and phagocytosis in murine RAW264.7 macrophage cell line.
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Extracellular cleavage of the p75 neurotrophin receptor is implicated in its pro-survival effect in breast cancer cells.
FEBS Lett.
PUBLISHED: 04-24-2013
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The p75 neurotrophin receptor (p75NTR) undergoes sequential proteolytic cleavages leading to the generation of a carboxyl-terminal fragment (p75NTR-CTF) and an intracellular domain (p75NTR-ICD) in many cellular models. We have previously shown that p75NTR is involved in the survival of breast cancer cells. Here, we demonstrated that p75NTR cleavage occurs also in these cells. Surprisingly, p75NTR-CTF increased cell survival, whereas p75NTR-ICD had no effect. The pro-survival effect of p75NTR-CTF was associated with a decrease of TNF-related apoptosis-inducing ligand (TRAIL)-induced PARP and caspase 3 cleavages. Finally, our findings indicate that p75NTR could favor cell survival via its carboxyl-terminal fragment, independently of PI3-kinase, NF-?B, or MAP kinase signaling pathways.
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Reference standards for forced expiratory indices in Chinese preschool children.
Pediatr. Pulmonol.
PUBLISHED: 02-08-2013
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Spirometric testing is traditionally achievable in children of school-age and beyond. Incorporation of interactive incentives motivates preschool children to facilitate measurement of forced expiratory indices. Validated spirometric reference standards are available for Caucasian preschoolers but lacking in Asians. We established spirometric references in Chinese children aged 2-7 years, who were recruited from 19 randomly selected nurseries and kindergartens in Hong Kong. Parents completed International Study of Asthma and Allergies in Childhood questionnaire, and children concurrently performed incentive spirometry on-site according to international guideline. Prediction equations for spirometric indices were formulated by linear regression. One thousand four hundred two (72.9%) of 1,922 consented children, with mean (SD) age 4.4 (1.0) years, successfully performed spirometry. Following exclusions due to medical and technical reasons, 895 (63.8%) children contributed spirometric data to our references. Girls had lower FEV0.5 , FEV0.75 , FEV1 , FVC, and PEF but similar FEF25-75 than boys, adjusted for age, weight, and standing height as covariates. Standing height was the most important predictor for FEV0.5 , FEV0.75 , FEV1 , FVC, and PEF in both boys (adjusted R(2) 0.525-0.734) and girls (adjusted R(2) 0.583-0.721), whereas the best prediction model for both gender is formed by standing height, weight, and age. At various standing heights, our preschoolers had FEV1 Z-scores 0.13-1.00 higher than those of collaborative Caucasian reference. This study justifies the need for ethnic-specific reference equations and presents spirometry references in young Chinese children. Their forced expiratory indices are determined by gender, age, weight and standing height, and standing height is the best anthropometric index to predict all spirometric indices.
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Correlation of hepatitis B surface antigen level with response to telbivudine in naive patients with chronic hepatitis B.
Hepatol. Res.
PUBLISHED: 01-31-2013
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AIM: Hepatitis B surface antigen (HBsAg) has become a marker to judge immunological response to hepatitis B therapy. Quantified serum HBsAg levels can predict the response to pegylated interferon and entecavir. In this study, we aimed to explore the correlation of serum HBsAg levels with response to telbivudine (LdT) treatment in patients with chronic hepatitis B (CHB). METHODS: Seventy-three treatment-naive CHB patients were recruited and received LdT monotherapy for 52 weeks and serial HBsAg levels were measured at five protocol time points. According to therapeutic efficacy at week 52, three subgroups of patients were identified, including complete responders (CR), partial responders (PR) and non-responders (NR). RESULTS: After 52 weeks of treatment, CR, PR and NR represented 19 (26%), 33 (45%) and 21 (29%) patients in the sample of 73, respectively. The median values of baseline HBsAg (log10 IU/mL) were 4.05, 4.50 and 5.03 for CR, PR and NR, respectively. There was a distinct decline of HBsAg at week 52; median log10 HBsAg levels (IU/mL) were 3.61 (CR), 3.86 (PR) and 4.31 (NR). Positive correlation between HBsAg levels and HBV DNA loads was observed in the group of NR and early antiviral treatment of PR, but not in CR. CONCLUSION: Initial HBsAg level was closely correlated with the efficacy of LdT. Patients with low HBsAg levels presented satisfactory responses. Therefore, initial level and correlation with HBV DNA of the serum HBsAg levels could predict responsiveness in CHB patients receiving LdT.
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LMP1-induced cell death may contribute to the emergency of its oncogenic property.
PLoS ONE
PUBLISHED: 01-01-2013
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Epstein-Barr Virus (EBV) Latent Membrane Protein 1 (LMP1) is linked to a variety of malignancies including Hodgkins disease, lymphomas, nasopharyngeal and gastric carcinoma. LMP1 exerts its transforming or oncogenic activity mainly through the recruitment of intracellular adapters via LMP1 C-terminal Transformation Effector Sites (TES) 1 and 2. However, LMP1 is also reported to elicit significant cytotoxic effects in some other cell types. This cytotoxic effect is quite intriguing for an oncogenic protein, and it is unclear whether both functional aspects of the protein are related or mutually exclusive.
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Proteome changes induced by overexpression of the p75 neurotrophin receptor (p75NTR) in breast cancer cells.
Int. J. Dev. Biol.
PUBLISHED: 12-14-2011
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In breast cancer cells, the neurotrophin receptor p75(NTR) acts as a prosurvival factor able to stimulate resistance to apoptosis, but its mechanism of action remains incompletely defined. In this study, we investigated the global proteome modification induced by p75(NTR) overexpression in breast cancer cells treated by the pro-apoptotic agent tumor necrosis factor (TNF)-related-apoptosis-inducing-ligand (TRAIL). p75(NTR) was stably overexpressed in the MCF-7 breast cancer cells and the impact of a treatment by TRAIL was investigated in wild type vs. p75(NTR) overexpressing cells. Proteins were separated in two-dimensional electrophoresis, and regulated spots were detected by computer assisted analysis before identification by MALDI-TOF/TOF mass spectrometry. In the absence of TRAIL treatment, p75(NTR) did not induce any change in the proteome of breast cancer cells. In contrast, after treatment with TRAIL, fragments of cytokeratin-8, -18 and -19, as well as full length cytokeratin-18, were up-regulated by p75(NTR) overexpression. Of note, spectrin alpha-chain and the ribosomal protein RPLP0 were induced by TRAIL, independently of p75(NTR) level. Interestingly, the well known stress-induced protein HSP-27 was less abundant when p75(NTR) was overexpressed, indicating that p75(NTR) overexpression reduced TRAIL induced cell stress. These data indicate that overexpression of p75(NTR) induces proteome modifications in breast cancer cells and provide information on how this receptor contributes in tumor cell resistance to apoptosis.
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Pro-nerve growth factor induces autocrine stimulation of breast cancer cell invasion through tropomyosin-related kinase A (TrkA) and sortilin protein.
J. Biol. Chem.
PUBLISHED: 11-29-2011
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The precursor of nerve growth factor (proNGF) has been described as a biologically active polypeptide able to induce apoptosis in neuronal cells, via the neurotrophin receptor p75(NTR) and the sortilin receptor. Herein, it is shown that proNGF is produced and secreted by breast cancer cells, stimulating their invasion. Using Western blotting and mass spectrometry, proNGF was detected in a panel of breast cancer cells as well as in their conditioned media. Immunohistochemical analysis indicated an overproduction of proNGF in breast tumors, when compared with benign and normal breast biopsies, and a relationship to lymph node invasion in ductal carcinomas. Interestingly, siRNA against proNGF induced a decrease of breast cancer cell invasion that was restored by the addition of non-cleavable proNGF. The activation of TrkA, Akt, and Src, but not the MAP kinases, was observed. In addition, the proNGF invasive effect was inhibited by the Trk pharmacological inhibitor K252a, a kinase-dead TrkA, and siRNA against TrkA sortilin, neurotensin, whereas siRNA against p75(NTR) and the MAP kinase inhibitor PD98059 had no impact. These data reveal the existence of an autocrine loop stimulated by proNGF and mediated by TrkA and sortilin, with the activation of Akt and Src, for the stimulation of breast cancer cell invasion.
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[Progress in microalgae culture system for biodiesel combined with reducing carbon dioxide emission].
Sheng Wu Gong Cheng Xue Bao
PUBLISHED: 11-29-2011
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Wastewater resources, CO2 emission reduction and microalgae biodiesel are considered as current frontier fields of energy and environmental researches. In this paper, we reviewed the progress in system of microalgae culture for biodiesel production by wastewater and stack gas. Multiple factors including microalgal species, nutrition, culture methods and photobioreactor, which were crucial to the cultivation of microalgae for biodiesel production, were discussed in detail. A valuable culture system of microalgae for biodiesel production or other high value products combined with the treatment of wastewater by microalgae was put forward through the optimizations of algal species and culture technology. The culture system coupled with the treatment of wastewater, the reduction of CO2 emission with the cultivation of microalgae for biodiesel production will reduce the production cost of microalgal biofuel production and the treatment cost of wastewater simultaneously. Therefore, it would be a promising technology with important environmental value, social value and economic value to combine the treatment of wastewater with the cultivation of microalgae for biodiesel production.
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Fabrication of micro-nano structure nanofibers by solvent etching.
J Nanosci Nanotechnol
PUBLISHED: 11-23-2011
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Micro-Nano structure nanofibrous affinity membranes of poly(ether sulfones) (PES) blended with a functional polymer poly(ethyleneimine) (PEI) were fabricated by electrospinning technique followed by solvent etching in crosslinking solution. The surface SEM image of the water washed PES/PEI nanofibrous membrane confirmed that PEI was concentrated on the fiber surface. The nanofibrous PES/PEI membranes were crosslinked in a mixture of acetone and water with glutaraldehyde (crosslinking agent, GA), and the micro-nano structural surface of the nanofibrous membranes was created by solvent etching due to the solvation between PEI and the solvent water in the crosslinking solution during the crosslinking process. The influence of the component of the crosslinking bath on the mophology of the resulting PES/PEI nanofibers was investigated. It was found that the relatively uniform micro-nano spherules grew on the surface of the nanofibers when the content of water in crosslinking solution was more than 20 wt%, and the diameters of the spherules were in the range of 50-250 nm. The advantage of the micro-nano structrue for the heavy metal ions removal in wastwater has been demonstrated by taking a series of static adsorption experiments. It was found that the micro-nano structrue of PES/PEI nanofibrous membranes could bring high performance of adsorption capacity for heavy metal ions, indicating that the unique morphology could bring much more large surface area per unit mass and high effectivity for heavy metal ions removal from aqueous solutions.
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2-Meth-oxy-N-[(S)-3-methyl-butan-2-yl]-6-{[(S)-3-methyl-butan-2-yl]amino}-3,5-dinitro-benzamide.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 11-13-2011
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The title compound, C(18)H(28)N(4)O(6), crystallizes with two mol-ecules in the asymmetric unit which differ slightly in conformation. The dihedral angle between the amide plane and the benzene ring are 72.6?(2) and 66.8?(2)° in the two mol-ecules. A strong intra-molecular N-H?O hydrogen bond between the amino and nitro groups occurs in each mol-ecule. The crystal structure features two symmetry-independent polymeric chains along [010] generated by N-H?O hydrogen bonds between the amide groups.
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Amino acid substitution in NPC1 that abolishes cholesterol binding reproduces phenotype of complete NPC1 deficiency in mice.
Proc. Natl. Acad. Sci. U.S.A.
PUBLISHED: 09-06-2011
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Substitution mutations in adjacent amino acids of the N-terminal domain of NPC1, a lysosomal membrane protein, abolish its cholesterol binding activity and impair its ability to export cholesterol from lysosomes of cultured cells lacking npc1 [Kwon HJ, et al. (2009) Cell 137:1213-1224]. Here, we show that the same two mutations (proline-202 and phenylalanine-203, both changed to alanine) reproduce the phenotype of complete NPC1 deficiency when knocked into the mouse npc1 gene by homologous recombination. Homozygous npc1(pf/pf) mice exhibited neurodegeneration beginning at day 49 and died at a median age of 84 d, as previously reported for mice that lack npc1. Liver and other organs of the npc1(pf/pf) mice accumulated excess cholesterol in lysosomes. In liver, mRNAs encoding several lysosomal proteins were elevated, including NPC1 and NPC2 and several digestive enzymes (acid lipase, ?-glucuronidase, and cathepsins B and D). Weekly treatment with hydroxypropyl-?-cyclodextrin (HPCD) beginning at 7 wk reduced hepatic cholesterol accumulation and diminished the lysosomal mRNAs. We conclude that the cholesterol binding site in the N-terminal domain of NPC1 is essential for cholesterol export from lysosomes in living animals as it is in cultured cells. The HPCD-mediated reduction of excess lysosomal enzymes may contribute to the ability of this drug to delay the progression of NPC disease in mice.
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[Preparation and anti-oxidant activity of cinnamic acid derivatives-g-CTS].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 08-17-2011
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To prepare cinnamic acid derivatives-g-CTS and to study its antioxidation activity.
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Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4? through a Peroxisome Proliferator-Activated Receptor ?-Dependent Mechanism in Liver.
PPAR Res
PUBLISHED: 08-10-2011
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Hepatic nuclear factor 4? (HNF4?) modulates the transcriptional activation of numerous metabolic genes in liver. In this study, gene-array analysis revealed that HNF4? overexpression increased peroxisome proliferator-activated receptor? (PPAR?) greatly in cultured rat primary hepatocytes. PPAR-response-element-driven reporter gene expression could be elevated by HNF4?. Bioinformatics analysis revealed a high-affinity HNF4? binding site in the human PPAR?2 promoter and in vitro experiments showed that this promoter could be transactivated by HNF4?. The presence of HNF4? on the promoter was then confirmed by ChIP assay. In vivo, hepatic overexpression of HNF4? decreased cholesterol levels both in plasma and liver and several hepatic genes related to cholesterol metabolism, including scavenger receptor BI (SR-BI), were upregulated. The upregulation of SR-BI by HNF4? could be inhibited by a PPAR? antagonist in vitro. In conclusion, HNF4? regulates cholesterol metabolism in rat by modulating the expression of SR-BI in the liver, in which the upregulation of PPAR? was involved.
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Understanding barriers to cataract surgery among older persons in rural China through focus groups.
Ophthalmic Epidemiol
PUBLISHED: 07-26-2011
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To utilize focus groups (FGs) to identify barriers to cataract surgery specific to older persons in rural Guangdong, China.
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Protective effect of tetrahydroxystilbene glucoside on 6-OHDA-induced apoptosis in PC12 cells through the ROS-NO pathway.
PLoS ONE
PUBLISHED: 07-11-2011
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Oxidative stress plays an important role in the pathogenesis of neurodegenerative diseases, such as Parkinsons disease. The molecule, 2,3,5,4-tetrahydr- oxystilbene-2-O-?-D-glucoside (TSG), is a potent antioxidant derived from the Chinese herb, Polygonum multiflorum Thunb. In this study, we investigated the protective effect of TSG against 6-hydroxydopamine-induced apoptosis in rat adrenal pheochromocytoma PC12 cells and the possible mechanisms. Our data demonstrated that TSG significantly reversed the 6-hydroxydopamine-induced decrease in cell viability, prevented 6-hydroxydopamine-induced changes in condensed nuclei and decreased the percentage of apoptotic cells in a dose-dependent manner. In addition, TSG slowed the accumulation of intracellular reactive oxygen species and nitric oxide, counteracted the overexpression of inducible nitric oxide syntheses as well as neuronal nitric oxide syntheses, and also reduced the level of protein-bound 3-nitrotyrosine. These results demonstrate that the protective effects of TSG on rat adrenal pheochromocytoma PC12 cells are mediated, at least in part, by the ROS-NO pathway. Our results indicate that TSG may be effective in providing protection against neurodegenerative diseases associated with oxidative stress.
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What should the government do regarding health policy-making to develop community health care in Shanghai?
Int J Health Plann Manage
PUBLISHED: 07-08-2011
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The traditional three-stratum healthcare system, within which municipal, district and community hospitals all paid great attention to improving medical treatment service by developing medical technology, is no longer able to meet the current health needs in Shanghai. In 1997, the Chinese government called for the development of community health services to serve as a basic platform to provide public health service and basic medical cure. However, because the market-oriented economic reform was based on a fee-for-service mechanism (without a strict monitoring system), most community health centers (CHCs) still put great effort into developing medical services geared to profit, rather than to provide proper medical service for all and a "quality" public health service. To try to solve the problem, some government-controlled payment (GCP) system has been implemented in CHCs gradually in districts of Shanghai. The study intended to evaluate the impact of GCP solutions already implemented, as well as the impact of the standardized GCP system with supplementary solutions, in enabling CHCs to focus on providing quality public health services and appropriate medical treatment, rather than focusing on profit and loss, in order to meet the health needs aroused by major socioeconomic transition in Shanghai.
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Profiles of discontinuation and switching of thiazide diuretics: a cohort study among 9398 Chinese hypertensive patients.
Hypertens. Res.
PUBLISHED: 05-12-2011
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This study tested the hypothesis that younger, male patients or new clinic visitors, who were prescribed thiazide diuretics were more likely to have drug discontinuation and switching. All adult patients who visited any primary care clinic in one territory of Hong Kong, and who were prescribed a thiazide diuretic from January 2004 to June 2007 were included. The rates of discontinuation and switching, separately, 180 days after thiazide prescriptions were measured. Factors associated with discontinuation and switching were evaluated by multiple regression analyses. Among 9398 patients, 12.5% discontinued and 10.8% switched their prescriptions, whereas prescriptions of other patients remained the same. Younger patients (< 50 years (reference value); adjusted odds ratio (AOR) for 50-59 years=0.74, 95% confidence interval (CI) 0.61-0.90, P=0.002; AOR (60-69 years)=0.57, 95% CI 0.46-0.70, P < 0.001; AOR (? 70 years)=0.88, 95% CI 0.73-1.06, P=0.174), male subjects (AOR=1.23, 95% CI 1.07-1.40, P=0.003) and new visitors (AOR (repeat visitors)=0.55, 95% CI 0.47-0.65, P < 0.001) were more likely to be discontinuers. These associations between younger age (< 50 years (reference value); AOR (50-59) years=0.85, 95% CI 0.70-1.04, P=0.112; AOR (60-69 year)=0.79, 95% CI 0.65-0.98, P=0.028; AOR (? 70 years)=0.70, 95% CI 0.57-0.85, P < 0.001), male gender (AOR=1.29, 95% CI 1.12-1.48, P < 0.001) and new visitors (AOR (repeat visitors)=0.57, 95% CI 0.48-0.67, P < 0.001) were also significant for medication switching. Clinicians should monitor the medication-taking behavior more closely among patients aged 50-59 years, male subjects and new clinic visitors when thiazide was prescribed.
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Dynamic variations in the peripheral blood lymphocyte subgroups of patients with 2009 pandemic H1N1 swine-origin influenza A virus infection.
Virol. J.
PUBLISHED: 05-10-2011
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Novel Influenza A (H1N1) is an acute respiratory infectious disease. Animal experiments indicated that when H1N1 virus infected early hosts, it showed strong CD4(+), CD8(+), and CD4(+)CD25(+) T cell reactions. The aim of this study was to investigate the dynamic fluctuations of the peripheral blood lymphocyte subgroups in patients infected with H1N1 swine-origin influenza A virus (S-OIV).
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Mutations in hepatitis B virus DNA from patients with coexisting HBsAg and anti-HBs.
J. Clin. Virol.
PUBLISHED: 02-25-2011
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The serological markers with coexistence of hepatitis B surface antigen (HBsAg) and antibody to HBsAg (anti-HBs) of hepatitis B virus (HBV) infection were rare pattern. The virological significance, immune response and clinical outcome of these patients remain largely unknown.
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Brain-derived neurotrophic factor and neurotrophin-4/5 are expressed in breast cancer and can be targeted to inhibit tumor cell survival.
Clin. Cancer Res.
PUBLISHED: 02-24-2011
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Given that nerve growth factor has previously been shown to be involved in breast cancer progression, we have tested here the hypothesis that the other neurotrophins (NT) are expressed and have an influence in breast tumor growth.
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Organic anion and cation transporters are possibly involved in renal excretion of entecavir in rats.
Life Sci.
PUBLISHED: 02-22-2011
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The purpose of the present study was to investigate the roles of transporters in the renal excretion of entecavir.
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Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR.
J. Clin. Microbiol.
PUBLISHED: 01-26-2011
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A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.
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Effect of telbivudine therapy on the cellular immune response in chronic hepatitis B.
Antiviral Res.
PUBLISHED: 01-10-2011
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Weak T-cell reactivity to the hepatitis B virus (HBV) is believed to be the dominant cause of chronic HBV infection. Several lines of experimental evidence suggest that treatment with telbivudine increases the rate of HBV e antigen (HBeAg) loss, undetectable HBV DNA, and normalization of serum alanine aminotransferase (ALT) in chronic hepatitis B patients (CHB). However, it is still unclear how early antiviral therapy affects cellular immune responses during sustained telbivudine treatment. In order to investigate this issue, we measured detailed prospective clinical, virological, and biochemical parameters, and we examined the frequency of T cell subgroups as well as the ability of peripheral blood mononuclear cells (PBMC) to respond to stimuli at five protocol time points for 51 CHB patients who received telbivudine therapy for one year. The preliminary data from this study revealed that effective-treated patients showed an increased frequency of peripheral blood CD4(+)T lymphocytes, an augmented proliferative response of HBV-specific T-cells to the hepatitis B core antigen (HBcAg), and the induction of cytokines, such as interferon gamma (IFN-?), tumour necrosis factor alpha (TNF-?) release at the site of infection compared to non-responsive patients. Enhanced HBV-specific T-cell reactivity to telbivudine therapy, which peaked at treatment week 12, was confined to a subgroup of effective-treated patients who achieved greater viral suppression.
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Corneal biomechanics, refractive error, and axial length in Chinese primary school children.
Invest. Ophthalmol. Vis. Sci.
PUBLISHED: 01-01-2011
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Low corneal hysteresis is associated with longer axial length in Chinese secondary school children. The authors sought to explore this association in primary school children.
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GD? synthase expression enhances proliferation and tumor growth of MDA-MB-231 breast cancer cells through c-Met activation.
Mol. Cancer Res.
PUBLISHED: 10-01-2010
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The disialoganglioside G(D3) is overexpressed in ?50% of invasive ductal breast carcinoma, and the G(D3) synthase gene (ST8SIA1) displays higher expression among estrogen receptor-negative breast cancer tumors, associated with a decreased overall survival of breast cancer patients. However, no relationship between ganglioside expression and breast cancer development and aggressiveness has been reported. We have previously shown that overexpression of G(D3) synthase induces the accumulation of b- and c-series gangliosides (G(D3), G(D2), and G(T3)) at the cell surface of MDA-MB-231 breast cancer cells together with the acquisition of a proliferative phenotype in the absence of serum. Here, we show that phosphoinositide 3-kinase/Akt and mitogen-activated protein kinase/extracellular signal-regulated kinase pathways are constitutively activated in G(D3) synthase-expressing cells. Analysis of phosphorylation of tyrosine kinase receptors shows a specific c-Met constitutive activation in G(D3) synthase-expressing cells, in the absence of its ligand, hepatocyte growth factor/scatter factor. In addition, inhibition of c-Met or downstream signaling pathways reverses the proliferative phenotype. We also show that G(D3) synthase expression enhances tumor growth in severe combined immunodeficient mice. Finally, a higher expression of ST8SIA1 and MET in the basal subtype of human breast tumors are observed. Altogether, our results show that G(D3) synthase expression is sufficient to enhance the tumorigenicity of MDA-MB-231 breast cancer cells through a ganglioside-dependent activation of the c-Met receptor.
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Synthesis and antitumor activity of 2-aryl-1, 2, 4-triazolo[1, 5-a] pyridine derivatives.
Med Chem
PUBLISHED: 04-06-2010
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A novel series of 2-aryl-1, 2, 4-triazolo [1, 5-a] pyridine derivatives have been synthesized and evaluated for their cytotoxic activities in vitro against Human ovarian cancer cell line (HO-8910) and Human liver cancer cell line (Bel 7402). Most compounds showed high or mediate activity against the cancer cell lines when compared with Cisplatin. Two of them were tested the apoptosis on Bel 7402.
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Overexpression of p75(NTR) increases survival of breast cancer cells through p21(waf1).
Cell. Signal.
PUBLISHED: 03-31-2010
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The p75 neurotrophin receptor (p75(NTR)) plays a critical role in various neuronal and non-neuronal cell types by regulating cell survival, differentiation and proliferation. To evaluate the influence of p75(NTR) in breast cancer development, we have established and characterized breast cancer cells which stably overexpress p75(NTR). We showed that p75(NTR) overexpression per se promoted cell survival to apoptogens with a concomitant slowdown of cell growth. The pro-survival effect is associated with an increased expression of the inhibitor of apoptosis protein-1 (c-IAP1), a decrease of TRAIL-induced cleavage of PARP, procaspase 9 and procaspase 3, and a decrease of cytochrome C release from the mitochondria. The anti-proliferative effect is due to a cell accumulation in G0/G1, associated with a decrease of Rb phosphorylation and an increase of p21(waf1). Interestingly, inhibition of p21(waf1) with siRNA not only restores proliferation but also abolishes the pro-survival effect of p75(NTR), indicating the key role of p21(waf1) in the biological functions of p75(NTR). Finally, using a SCID mice xenograft model, we showed that p75(NTR) overexpression favors tumor growth and strongly increases tumor resistance to anti-tumoral treatment. Together, our findings suggest that p75(NTR) overexpression in breast tumor cells could favor tumor survival and contribute to tumor resistance to drugs. This provides a rationale to consider p75(NTR) as a potential target for the future design of innovative therapeutic strategies.
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Ku86 is important for TrkA overexpression-induced breast cancer cell invasion.
Proteomics Clin Appl
PUBLISHED: 03-26-2010
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We have recently shown that breast tumors express high levels of TrkA compared with normal breast tissues, with TrkA overexpression enhancing breast cancer cell invasion in vitro and metastasis in animal models. In this study, we tried to identify molecules involved in TrkA overexpression-mediated biological effects in breast cancer cells.
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Nerve growth factor promotes breast cancer angiogenesis by activating multiple pathways.
Mol. Cancer
PUBLISHED: 03-26-2010
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Although several anti-angiogenic therapies have been approved in the treatment of cancer, the survival benefits of such therapies are relatively modest. Discovering new molecules and/or better understating signaling pathways of angiogenesis is therefore essential for therapeutic improvements. The objective of the present study was to determine the involvement of nerve growth factor (NGF) in breast cancer angiogenesis and the underlying molecular mechanisms.
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Ghrelin O-acyltransferase (GOAT) is essential for growth hormone-mediated survival of calorie-restricted mice.
Proc. Natl. Acad. Sci. U.S.A.
PUBLISHED: 03-15-2010
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Ghrelin O-acyltransferase (GOAT) attaches octanoate to proghrelin, which is processed to ghrelin, an octanoylated peptide hormone that stimulates release of growth hormone (GH) from pituitary cells. Elimination of the gene encoding ghrelin or its receptor produces only mild phenotypes in mice. Thus, the essential function of ghrelin is obscure. Here, we eliminate the Goat gene in mice, thereby eliminating all octanoylated ghrelin from blood. On normal or high fat diets, Goat(-/-) mice grew and maintained the same weights as wild-type (WT) littermates. When subjected to 60% calorie restriction, WT and Goat(-/-) mice both lost 30% of body weight and 75% of body fat within 4 days. In both lines, fasting blood glucose initially declined equally. After 4 days, glucose stabilized in WT mice at 58-76 mg/dL. In Goat(-/-) mice, glucose continued to decline, reaching 12-36 mg/dL on day 7. At this point, WT mice showed normal physical activity, whereas Goat(-/-) mice were moribund. GH rose progressively in calorie-restricted WT mice and less in Goat(-/-) mice. Infusion of either ghrelin or GH normalized blood glucose in Goat(-/-) mice and prevented death. Thus, an essential function of ghrelin in mice is elevation of GH levels during severe calorie restriction, thereby preserving blood glucose and preventing death.
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Intake of fiber and nuts during adolescence and incidence of proliferative benign breast disease.
Cancer Causes Control
PUBLISHED: 02-25-2010
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We examined the association between adolescent fiber intake and proliferative BBD, a marker of increased breast cancer risk, in the Nurses Health Study II.
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Effect of regulatory T cells and adherent cells on the expansion of HBcAg-specific CD8+ T cells in patients with chronic hepatitis B virus infection.
Cell. Immunol.
PUBLISHED: 01-19-2010
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Patients with chronic HBV infection show poor immune response to HBV-specific CD8+ T cells. Several studies demonstrate that regulatory T cells (Treg) and dendritic cells (DC) are important to maintain peripheral immune tolerance. In this study, we investigated the effects of CD4+CD25+Treg and/or the adherent cells (AC) on the proliferation of HBc18-27-specific CD8+ T cells (c18-27-CD8Ts) in response to in vitro stimulation. The frequency of c18-27-CD8Ts in four different mixed leukocyte reactions (MLRs) were analyzed using an HLA-A2-HBc18-27 tetramer. The data indicated that the median percentage of c18-27-CD8Ts in four different MLRs were significant difference in patients with chronic HBV infection. Our results showed that Treg and/or AC might suppress the frequency of HBc18-27-specific CD8+ T cell proliferation in response to in vitro stimulation in chronic HBV patients, and AC might be more effective than Treg.
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Individuals at risk of beta-blocker discontinuation: a cohort study in 19,177 Chinese patients.
Clin Res Cardiol
PUBLISHED: 01-18-2010
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This cohort study evaluated the factors associated with discontinuation of antihypertensive pharmacotherapy among Chinese patients who were prescribed beta-blockers. We tested the hypothesis that patients age, gender, socioeconomic status, clinical settings and the number of comorbidities were significantly associated with the discontinuation of beta-blockers.
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Role of endothelial progenitor cells in breast cancer angiogenesis: from fundamental research to clinical ramifications.
Breast Cancer Res. Treat.
PUBLISHED: 11-03-2009
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Blood vessel formation (neovascularization) in tumors can occur through two mechanisms: angiogenesis and vasculogenesis. Angiogenesis results from proliferation and sprouting of existing blood vessels close to the tumor, while vasculogenesis is believed to arise from recruitment of circulating cells, largely derived from the bone marrow, and de novo clonal formation of blood vessels from these cells. Increasing evidence in animal models indicate that bone marrow-derived endothelial precursor cells (EPC) can contribute to tumor angiogenesis. This review aims to collate existing literature and provide an overview on the current knowledge of EPC involvement in breast cancer angiogenesis. We also discuss recent attempts to use EPC as biomarker and therapeutic target in clinical trials.
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Babesia sp. BQ1 (Lintan): molecular evidence of experimental transmission to sheep by Haemaphysalis qinghaiensis and Haemaphysalis longicornis.
Parasitol. Int.
PUBLISHED: 10-01-2009
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Ovine babesiosis is an economically important disease induced by tick transmitted haemoparasites throughout the world. In China, several ovine Babesia strains have been isolated from field-collected ticks or sheep blood during the last two decades but little is known about the vector ticks and transmission pattern. Babesia sp. BQ1 (Lintan) is a Babesia strain infective for sheep and goats, isolated from blood of sheep experimentally infested with Haemaphysalis qinghaiensis collected in field. In the present study, we explored the experimental transmission of Babesia sp. BQ1 (Lintan) to sheep by H. qinghaiensis and Haemaphysalis longicornis. Based on the evidence from nested PCR, it suggested that H. qinghaiensis and H. longicornis are the potential vector ticks of Babesia sp. BQ1 (Lintan) and that larvae, nymphs and adults of both tick species were able to transmit Babesia sp. BQ1 (Lintan) to sheep. Parasites could be detected in the blood, by specific nested PCR, for one month post-infestation.
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Chinese medicinal herbs in relieving perimenopausal depression: a randomized, controlled trial.
J Altern Complement Med
PUBLISHED: 09-23-2009
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To explore the effects of GengNianLe (GNL, also called perimenopausal depression relieving formula), a defined formula of Chinese medicinal herbs in relieving perimenopausal depression in Chinese women.
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Influence of usage history, instrument complexity, and different cleaning procedures on the cleanliness of blood-contaminated dental surgical instruments.
Infect Control Hosp Epidemiol
PUBLISHED: 06-06-2009
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Our study assessed the factors that influence the resistance of blood residues on dental surgical instruments to washer-disinfector-based cleaning procedures in a clinical setting. The use of 2 additional cleaning methods-presoaking and scrubbing by hand-and the use of newer and/or less structurally complex instruments significantly increased the efficacy of washer-disinfector cleaning.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.