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Find video protocols related to scientific articles indexed in Pubmed.
[Research progress of negative costimulator B7-H4 in urologic tumors].
Zhejiang Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 11-06-2014
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To compare the safety and efficacy of totally laparoscopic distal gastrectomy?TLDG?with laparoscopic assisted distal gastrectomy?LADG?for gastric cancer by meta-analysis. METHODS?The literature on comparative studies of TLDG and LADG up to June 2014 were extensively retrieved from database PubMed, Cochrane library, Web of Science, and Biosis Previews. The operation time?blood loss?time to flatus? time to first oral intake?postoperative hospital stay?postoperative morbidity?times of analgestic requirement?pain score?and the level of C-reactive protein?CRP? on postoperative day 1 and 7 were analyzed. The statistical analysis was performed with RevMan 5.1 software. RESULTS? Seven studies met the inclusion criteria for meta-analysis. A total of 1783 Patients were included for meta-analysis?among whom 727 cases underwent TLDG and 1056 underwent LADG. Comparing with LADG?TLDG experienced less blood loss?weighted mean difference?WMD?=22.86 ml?95% confidence interval?CI?: 12.0-33.72?P<0.01???less times of analgesic requirement?WMD=0.58?95% CI: 0.35-0.81? P< 0.01??less pain score on postoperative day 1 and day 3 ?day1: WMD=0.60?95% CI: 0.20-0.99?P < 0.01; day3: WMD=0.36?95% CI: 0.24-0.48?P < 0.01??earlier beginning to take diet?WMD=0.66?95% CI: 0.13-1.19?P=0.01?. The operation time?postoperative hospital stay?overall morbidity and anastomosis-related morbidity?and the level of CRP on postoperative day 1 and 7 were similar between two groups?Ps>0.05?. CONCLUSION?TLDG is a safe and feasible procedure with less blood loss? less pain? and quicker recovery than those of LADG.
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A rare case of mixed mucinous cystadenoma with serous cystadenoma of the pancreas treated by laparoscopic central pancreatectomy.
World J Surg Oncol
PUBLISHED: 10-01-2014
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Mixed mucinous cystadenoma with serous cystadenoma of the pancreas is rare. There have been only two previous case reports in the English-language literature. We present a case of a 46-year-old woman who was diagnosed with mixed mucinous cystadenoma with serous cystadenoma of the pancreas. Computed tomography and magnetic resonance imaging showed a cystic neoplasm in the dorsal/proximal body of the pancreas with a clear-margin multilocular cavity and enhanced internal septum. The patient underwent laparoscopic central pancreatectomy. The diagnosis of mixed mucinous cystadenoma with serous cystadenoma of the pancreas was confirmed by pathological examination. The patient was followed up for 3 months and there were no signs of recurrence, or pancreatic exocrine or endocrine insufficiency. To the best of our knowledge, this is the first reported case treated by laparoscopic central pancreatectomy.
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Developmental changes in children's understanding of horizontal projectile motion.
Int J Psychol
PUBLISHED: 09-03-2014
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This study investigated 5- to 13-year-old children's performance in solving horizontal projectile motion problems, in which they predicted the trajectory of a carried object released from a carrier in three different contexts. The results revealed that 5- and 8-year-olds' trajectory predictions were easily distracted by salient contextual features (e.g. the relative spatial locations between objects), whereas a proportion of 11- and 13-year-olds' performance suggested the engagement of the impetus concept in trajectory prediction. The impetus concept is a typical misconception of inertial motion that assumes that motion is caused by force. Children's performance across ages suggested that their naïve knowledge of projectile motion was neither well-developed and coherent nor completely fragmented. Instead, this study presented the dynamic process in which children with age gradually overcame the influences of contextual features and consistently used the impetus concept across motion problems.
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Heat shock protein expression enhances heat tolerance of reptile embryos.
Proc. Biol. Sci.
PUBLISHED: 08-01-2014
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The role of heat shock proteins (HSPs) in heat tolerance has been demonstrated in cultured cells and animal tissues, but rarely in whole organisms because of methodological difficulties associated with gene manipulation. By comparing HSP70 expression patterns among representative species of reptiles and birds, and by determining the effect of HSP70 overexpression on embryonic development and hatchling traits, we have identified the role of HSP70 in the heat tolerance of amniote embryos. Consistent with their thermal environment, and high incubation temperatures and heat tolerance, the embryos of birds have higher onset and maximum temperatures for induced HSP70 than do reptiles, and turtles have higher onset and maximum temperatures than do lizards. Interestingly, the trade-off between benefits and costs of HSP70 overexpression occurred between life-history stages: when turtle embryos developed at extreme high temperatures, HSP70 overexpression generated benefits by enhancing embryo heat tolerance and hatching success, but subsequently imposed costs by decreasing heat tolerance of surviving hatchlings. Taken together, the correlative and causal links between HSP70 and heat tolerance provide, to our knowledge, the first unequivocal evidence that HSP70 promotes thermal tolerance of embryos in oviparous amniotes.
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[Analysis of the role of endoscopic retrograde cholangiopancreatography in children with pancreaticobiliary diseases].
Zhonghua Er Ke Za Zhi
PUBLISHED: 06-28-2014
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To evaluate the safety and efficacy of endoscopic retrograde cholangiopancreatography (ERCP) in children with pancreaticobiliary diseases and the characteristics of pancreaticobiliary disorders in children.
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Three cases of laparoscopic total gastrectomy with intracorporeal esophagojejunostomy for gastric cancer in remnant stomach.
World J Surg Oncol
PUBLISHED: 05-01-2014
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Gastric cancer in remnant stomach is a rare tumor but with poor prognosis. Compared with conventional open surgery, laparoscopic gastrectomy has potential benefits for these patients due to advantages resulting from its minimally invasive approach. Herein, we report on three patients with gastric cancer in remnant stomach who underwent laparoscopic total gastrectomy with intracorporeal esophagojejunostomy successfully. The operative time was 280, 250 and 225 minutes, the estimated blood loss was 100, 80 and 50 ml and the length of postoperative hospital stay was seven, eight and nine days respectively. Our experience has suggested that laparoscopic total gastrectomy with intracorporeal esophagojejunostomy can be a safe, feasible and promising option for patients with gastric cancer in remnant stomach.
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Laparoscopic versus open resection for gastric gastrointestinal stromal tumors: an updated systematic review and meta-analysis.
World J Surg Oncol
PUBLISHED: 04-13-2014
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In past decades, laparoscopic surgery has been introduced for the treatment of gastrointestinal stromal tumors (GISTs). Recently, additional studies comparing laparoscopic versus open surgery for gastric GISTs have been published, and an updated meta-analysis of this subject is necessary.
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Totally laparoscopic gastrectomy for gastric cancer: A systematic review and meta-analysis of outcomes compared with open surgery.
World J. Gastroenterol.
PUBLISHED: 04-10-2014
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To systematically review the surgical outcomes of totally laparoscopic gastrectomy (TLG) vs open gastrectomy (OG) for gastric cancer.
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Laparoscopic spleen-preserving distal pancreatectomy for pancreatic neoplasms: A retrospective study.
World J. Gastroenterol.
PUBLISHED: 03-30-2014
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To describe the clinical characteristics, technical procedures, and outcomes of patients undergoing laparoscopic spleen-preserving distal pancreatectomy (LSPDP) for benign and malignant pancreatic neoplasms.
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Fever as a first manifestation of advanced gastric adenosquamous carcinoma: a case report.
World J. Gastroenterol.
PUBLISHED: 03-24-2014
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Gastric adenosquamous carcinoma (ASC) is a rare type of gastric cancer. It is a mixed neoplasm, consisting of glandular cells and squamous cells. It is often diagnosed at an advanced stage, thus carrying a poor prognosis. We describe a case of a 73-year-old male, who presented with refractory fever and an intra-abdominal mass on imaging. He underwent a laparoscopic exploration followed by a successful totally laparoscopic total gastrectomy with D2 lymphadenectomy for gastric cancer. Postoperative pathology revealed primary gastric ASC (T4aN0M0). The patient received adjuvant radiotherapy and chemotherapy with S1 and is alive 20 mo after surgery without recurrence. This is the first case of advanced gastric ASC with fever as the initial presentation treated with totally laparoscopic total gastrectomy reported in the English literature.
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Systematic review and meta-analysis of safety and efficacy of laparoscopic resection for gastrointestinal stromal tumors of the stomach.
Surg Endosc
PUBLISHED: 03-22-2014
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To evaluate the safety and efficacy of laparoscopic resection for gastrointestinal stromal tumors (GISTs) of the stomach with systematic review and meta-analysis.
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Laparoscopic ligation of proximal splenic artery aneurysm with splenic function preservation.
World J. Gastroenterol.
PUBLISHED: 03-05-2014
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Splenic artery aneurysm is one of the most common visceral aneurysms, and patients with this type of aneurysm often present without symptoms. However, when rupture occurs, it can be a catastrophic event. Although most of these aneurysms can be treated with percutaneous embolization, some located in uncommon parts of the splenic artery may make this approach impossible. We present a patient with an aneurysm in the proximal splenic artery, close to the celiac trunk, which was treated by laparoscopic ligation only, without resection of the aneurysm, and with long-term preservation of splenic function.
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Prenatal exposure to PFOS caused mitochondia-mediated apoptosis in heart of weaned rat.
Environ. Toxicol.
PUBLISHED: 02-17-2014
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Perfluorooctanyl sulfonate (PFOS), a cardiac toxicity compound, has been widely detected in the environment and in organisms. However, the toxic mechanism is not clear. Our previous study indicated that prenatal PFOS exposure led to swollen mitochondrial with vacuolar structure and loss of cristae in offsping's heart. The purpose of this study was to investigate the effect of PFOS on the apoptosis in developing heart and mitochondria-mediated apoptosis pathway. Pregnant Sprague-Dawley (SD) rats were exposed to PFOS at doses of 0.1, 0.6, and 2.0 mg/kg-d and 0.05% Tween 80 as control by gavage from gestation day 2 (GD 2) to GD 21. Apoptosis, as well as expression of apoptosis related genes associated with mitochondrial-mediated apoptosis pathway, including p53, bcl-2, bax, cytochrome c, caspase-9, and caspase-3 were analyzed in heart tissues from weaned (postnatal day 21, PND 21) offspring. The results showed that prenatal PFOS exposure resulted in apoptosis in the offspring's heart. The mRNA and protein expression levels of p53, bax, cytochrome c, caspase-9, and caspase-3 in the offspring's heart were enhanced in various PFOS-treated groups, meanwhile, the bcl-2 expression levels were decreased. Our results indicated that prenatal PFOS exposure induced the apoptosis of weaned offspring rat heart tissue via mitochondria-mediated apoptotic pathway. © 2014 Wiley Periodicals, Inc. Environ Toxicol, 2014.
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Short-term surgical and long-term survival outcomes after laparoscopic distal gastrectomy with D2 lymphadenectomy for gastric cancer.
BMC Gastroenterol
PUBLISHED: 02-17-2014
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Laparoscopic distal gastrectomy (LDG) for gastric cancer has gradually gained popularity. However, the long-term oncological outcomes of LDG have rarely been reported. This study aimed to investigate the survival outcomes of LDG, and evaluate the early surgical outcomes of laparoscopy-assisted distal gastrectomy (LADG) and totally laparoscopic distal gastrectomy (TLDG).
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Laparoscopic resection of synchronous gastric cancer and primary small intestinal lymphoma: a case report.
World J. Gastroenterol.
PUBLISHED: 01-01-2014
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Synchronous gastric cancer and primary small intestinal lymphoma are extremely rare. A 49-year-old woman was referred to our hospital with a history of upper abdominal pain for two weeks and was diagnosed with synchronous cancer. During hospitalization, the patient underwent laparoscopic distal gastrectomy + resection of bilateral ovaries + partial resection of both small intestine and descending colon. Pathological examination revealed a synchronous cancer consisting of early gastric cancer with poorly differentiated adenocarcinoma located in mucosa, with lymph node metastasis (3+/29) (T1N1M0, stage IB); and diffuse large B cell lymphoma of small intestine involving descending colon and bilateral ovaries, with lymph node metastasis (2+/5) (Ann Arbor IIE). The patient recovered well, without any obvious complications and was discharged on post-operative day 7. The patient received six cycles of chemotherapy after operation. She has been doing well with no evidence of recurrence for 13 mo.
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[The diagnosis and treatment of pancreatic neuroendocrine neoplasms].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 12-17-2013
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To analyze the prognostic factors of pancreatic neuroendocrine neoplasms (PNEN).
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Can infants make transitive inferences?
Cogn Psychol
PUBLISHED: 10-31-2013
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Researchers have long been interested in the emergence of transitive reasoning abilities (e.g., if A>B and B>C, then A>C). Preschool-aged children are found to make transitive inferences. Additionally, nonhuman animals demonstrate parallel abilities, pointing to evolutionary roots of transitive reasoning. The present research examines whether 16-month-old infants can make transitive inferences about other peoples preferences. If an agent prefers object-A over B (A>B) and B over C (B>C), infants seem to reason that she also prefers A over C (A>C) (Experiment 1). Experiment 2 provides indirect evidence that a one-directional linear ordering of the three items (A>B>C) may have helped infants to succeed in the task. These and control results present the first piece of evidence that precursors of transitive reasoning cognitive abilities exist in infancy.
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Schistosomiasis combined with colorectal carcinoma diagnosed based on endoscopic findings and clinicopathological characteristics: a report on 32 cases.
Asian Pac. J. Cancer Prev.
PUBLISHED: 10-03-2013
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To improve understanding of the relationship between schistosome-related enteropathy and colorectal carcinoma with particular focus on endoscopic findings and clinicopathological characteristics of colonic schistosomiasis.
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Successful interventional radiological management of postoperative complications of laparoscopic distal pancreatectomy.
World J. Gastroenterol.
PUBLISHED: 08-11-2013
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During the past decade, laparoscopic distal pancreatectomy (LDP) has gained increasing acceptance in the surgical community as a viable treatment option for distal pancreatic lesions. However, the possible complication of post-LDP pancreatic leakage remains a challenge, because it may lead to a series of events resulting in intraperitoneal abscess formation, sepsis, pseudoaneurysm formation, and occasional fatal hemorrhage. Dealing with these complications is extremely difficult and not much experience has been reported to date. We report a case involving the aforementioned post-LDP complications successfully managed by interventional radiological techniques while avoiding reoperation. We conclude that these management options are attractive, safe and minimally invasive alternatives to standard protocols.
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Laparoscopic transduodenal local resection of periampullary neuroendocrine tumor: a case report.
World J. Gastroenterol.
PUBLISHED: 08-05-2013
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Studies on laparoscopic transduodenal local resection have not been readily available. Only three cases have been reported in the English-language literature. We describe herein a case of 25-year-old woman with periampullary neuroendocrine tumor (NET). Endoscopic ultrasonography revealed a duodenal papilla mass originated from the submucosa and close to the ampulla. The periampullary tumor was successfully managed with laparoscopic transduodenal local resection without any procedure-related complications. Pathological examination showed a NET (Grade 2) with negative margin. The patient was followed up for six months without signs of recurrence. This case suggests that laparoscopic transduodenal local resection is a feasible procedure in selected patients with periampullary tumor.
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[Predictive value of 3 methods in severity evaluation and prognosis of acute paraquat poisoning].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 08-03-2013
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To evaluate the predictive value of 3 methods, namely plasma paraquat concentration, severity index of paraquat poisoning (SIPP), and acute physiology and chronic health evaluation (APACHE II) in severity evaluation and prognosis of acute paraquat poisoning.
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[Meta-analysis of laparoscopic-assisted versus open distal gastrectomy for gastric cancer].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 08-02-2013
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To evaluate the safety and efficacy of laparoscopic-assisted distal gastrectomy (LADG) for gastric cancer through a meta-analysis of LADG versus open distal gastrectomy(ODG).
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Systematic review and meta-analysis of laparoscopic and open gastrectomy for advanced gastric cancer.
World J Surg Oncol
PUBLISHED: 06-10-2013
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The use of laparoscopic gastrectomy (LG) in advanced gastric cancer (AGC) remains a controversial topic, mainly because of doubts about its oncologic validity. This study is a systematic review and meta-analysis of the available evidence.
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Laparoscopic vs open distal pancreatectomy for solid pseudopapillary tumor of the pancreas.
World J. Gastroenterol.
PUBLISHED: 06-08-2013
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To compare short- and long-term outcomes of laparoscopic vs open distal pancreatectomy for solid pseudopapillary tumor (SPT) of the pancreas.
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[The clinical comparison of totally laparoscopic versus open total gastrectomy for gastric cancer].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 04-13-2013
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To investigate the feasibility, safety and oncological effect of totally laparoscopic total gastrectomy (TLTG).
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Systematic review and meta-analysis of laparoscopy-assisted and open total gastrectomy for gastric cancer.
World J. Gastroenterol.
PUBLISHED: 03-29-2013
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To evaluate the safety and efficacy of laparoscopy-assisted total gastrectomy (LATG) and open total gastrectomy (OTG) for gastric cancer.
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A study of the technique of western blot for diagnosis of lyme disease caused by Borrelia afzelii in China.
Biomed. Environ. Sci.
PUBLISHED: 02-22-2013
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To study the technique of Western blot for the diagnosis of Lyme disease caused by Borrelia afzelii in China and to establish the standard criteria by operational procedure.
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Development of ELISAs for the detection of urogenital chlamydia trachomatis infection targeting the pORF5 protein.
Biomed. Environ. Sci.
PUBLISHED: 02-22-2013
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To prepare antibodies against pORF5 plasmid protein of Chlamydia trachomatis and develop double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) for the detection of genital C. trachomatis infections.
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Isolated splenic metastases from gastric carcinoma: a case report and literature review.
World J. Gastroenterol.
PUBLISHED: 02-16-2013
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Isolated metastases to the spleen from gastric carcinoma is very rare. Only a few cases have been reported in the literature. We herein present a case of isolated splenic metastases in a 62-year-old man, occurring 12 mo after total gastrectomy for gastric carcinoma. The patient underwent a laparoscopic exploration, during which two lesions were found at the upper pole of the spleen, without involvement of other organs. A laparoscopic splenectomy was performed. Histological examination confirmed that the splenic tumor was a poorly differentiated adenocarcinoma similar to the primary gastric lesion. The postoperative course was uneventful and the patient has been well for 9 mo, with no tumor recurrence. The clinical data of 18 cases of isolated splenic metastases from gastric carcinoma treated by splenectomy were summarized after a literature review. To our knowledge, this is the first reported case of isolated splenic metastases undergoing laparoscopic splenectomy.
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Case-matched comparison of laparoscopy-assisted and open distal gastrectomy for gastric cancer.
World J. Gastroenterol.
PUBLISHED: 01-16-2013
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To compare short- and long-term outcomes of laparoscopy-assisted and open distal gastrectomy for gastric cancer.
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[Cloning and cellular localization of pORF8 plasmid protein of Chlamydia trachomatis].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 12-01-2011
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To clone the plasmid protein pORF8 of Chlamydia trachomatis and localize its expression in Chlamydia-infected cells.
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[Induction of proinflammatory cytokines and cell apoptosis by Chlamydia pneumoniae Cpn0810].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
PUBLISHED: 09-13-2011
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To expresse the Chlamydia pneumoniae Cpn0810 in E.coli BL21, and to study weather could it inducing proinflamatory cytokines including TNF-? and IL-6 in human monocytic (THP-1) and cell apoptosis.
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Molecular modeling of cytochrome b? with a single cytochrome c-like thioether linkage.
J Mol Model
PUBLISHED: 06-08-2011
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Bovine liver cytochrome b (5) (cyt b (5)), with heme bound noncovalently, has been converted into a cyt c-like protein (cyt b (5) N57C) by constructing a thioether linkage between the heme and the engineered cysteine residue. With no X-ray or NMR structure available, we herein performed a molecular modeling study of cyt b (5) N57C. On the other hand, using amino acid sequence information for a newly discovered member of the cyt b (5) family, domestic silkworm cyt b (5) (DS cyt b (5)), we predicted the protein structure by homology modeling in combination with MD simulation. The modeling structure shows that both Cys57 in cyt b (5) N57C, and Cys56, a naturally occurring cysteine in DS cyt b (5), have suitable orientations to form a thioether bond with the heme 4-vinyl group, as the heme is in orientation A. In addition to providing structural information that was not previously obtained experimentally, these modeling studies provide insight into the formation of cyt c-like thioether linkages in cytochromes, and suggest that c-type cyt b (5) maturation involves a b-type intermediate.
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Long-term effect of interferon treatment on the progression of chronic hepatitis B: Bayesian meta-analysis and meta-regression.
Hepatol. Res.
PUBLISHED: 04-19-2011
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Aim:? The long-term effects of interferon treatment on the progression of chronic hepatitis B (CHB) have been studied extensively, but its true clinical benefits and the predictors of its efficacy remain unclear. Methods:? A systematic published work search was undertaken. Eligible studies included those with interferon treatment and control groups, and with liver cirrhosis (LC), hepatocellular carcinoma (HCC) or death as main outcomes. Bayesian meta-analysis and meta-regression were performed to assess associations between interferon treatment and disease progression, and the impacts of potential covariates. Results:? Eleven articles met the inclusion criteria. LC, HCC and death were end-points in four, nine and six studies, respectively. In all studies, interferon was associated with significant preventive effects on HCC according to the DerSimonian-Laird method (relative risk [RR]?=?0.470, 95% confidence interval [CI]?=?0.260-0.850) and Bayesian method adjusting underlying risk (RR?=?0.249, 95% Bayesian credible intervals [BCI]?=?0.049-0.961), but not according to Bayesian meta-analysis (RR?=?0.274, 95% BCI?=?0.059-1.031); and it showed similar effects in death but not in LC. However, most of the high-quality studies never revealed protective benefits in these end-points. Bayesian meta-regression identified Asian ethnicity in death, higher hepatitis B e-antigen (HBeAg) seroconversion rate or positivity rate, and length of follow up (?5?years) in HCC as potentially protective against disease progression. Subgroup analysis confirmed similar effects from these factors in HCC and death. Conclusion:? Additional evidence is needed to support the role of interferon in delaying CHB progression.
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In vitro activities of erythromycin, tetracycline and levofloxacin alone and in dual combinations against ureaplasma spp.
Chemotherapy
PUBLISHED: 03-24-2011
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It was the aim of our study to evaluate the in vitro activities of tetracycline (TET), erythromycin (ERY) and levofloxacin (LVX) alone and in dual combinations against ureaplasmas.
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Interactions of uranyl ion with cytochrome b? and its His39Ser variant as revealed by molecular simulation in combination with experimental methods.
J Mol Model
PUBLISHED: 03-22-2011
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The biological toxicity of uranyl ion (UO (2) (2+) ) lies in interacting with proteins and disrupting their native functions. The structural and functional consequences of UO (2) (2+) interacting with cytochrome b (5) (cyt b (5)), a small membrane heme protein, and its heme axial ligand His39Ser variant, cyt b (5) H39S, were investigated both experimentally and theoretically. In experiments, although cyt b (5) was only slightly affected, UO (2) (2+) binding to cyt b (5) H39S with a K (D) of 2.5 ?M resulted in obvious alteration of the heme active site, and led to a decrease in peroxidase activity. Theoretically, molecular simulation proposed a uranyl ion binding site for cyt b (5) at surface residues of Glu37 and Glu43, revealing both coordination and hydrogen bonding interactions. The information gained in this study provides insights into the mechanism of uranyl toxicity toward membrane protein at an atomic level.
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Dynamics comparison of two myoglobins with a distinct heme active site.
J Mol Model
PUBLISHED: 01-25-2011
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Sperm whale myoglobin (swMb) is a well-studied heme protein, both experimentally and theoretically. Comparatively, little attention has been paid to another member of Mb family, Aplysia limacina myoglobin (apMb). swMb and apMb have the same overall structure and perform the same biological function, i.e., O(2) carrier, while using a distinct heme active site. To provide insights into the structure-function relationship for these two Mbs, we herein made a comparison in terms of their dynamics properties using molecular dynamics simulation. We analyzed the overall structure and protein motions, as well as the intramolecular contacts, namely salt-bridges and hydrogen bonds, especially the interactions between the heme propionate groups and the polypeptide chain. The internal cavities in apMb were also compared to the well-known xenon and other cavities in swMb. Based on current simulations, we propose a unique "arginine gate" for apMb, which has a similar function to the histidine gate observed for swMb in previous studies. This study provides valuable information for understanding the homology of heme proteins, and also aids in rational design of structural and functional heme proteins by alternating the heme active site.
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Laparoscopic wedge resection of synchronous gastric intraepithelial neoplasia and stromal tumor: a case report.
World J. Gastroenterol.
PUBLISHED: 10-19-2010
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Synchronous occurrence of epithelial neoplasia and gastrointestinal stromal tumor (GIST) in the stomach is uncommon. Only rare cases have been reported in the literature. We present here a 60-year-old female case of synchronous occurrence of gastric high-level intraepithelial neoplasia and GIST with the features of 22 similar cases and detailed information reported in the English-language literature summarized. In the present patient, epithelial neoplasia and GIST were removed en bloc by laparoscopic wedge resection. To the best of our knowledge, this is the first reported case treated by laparoscopic wedge resection.
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A novel mitochondrial tRNA(Val) T1658C mutation identified in a CPEO family.
Mol. Vis.
PUBLISHED: 05-06-2010
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To analyze mitochondrial DNA (mt DNA) gene mutations in a 19-year-old female patient, who presented with chronic progressive external ophthalmoplegia (CPEO), together with her mother and younger sister.
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[Totally laparoscopic gastrectomy for gastric cancer].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 04-07-2010
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To evaluate the feasibility and clinical efficacy of totally laparoscopic gastrectomy (TLG) for gastric cancer.
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[Polypropylene mesh wrap around reinforced pancreatojejunostomy for soft pancreas].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 08-13-2009
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To evaluate the preventive effect of a new anastomotic technique upon pancreatic leakage-polypropylene mesh wrap around reinforced pancreatojejunostomy on soft pancreas.
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Aortoduodenal fistula following aortic reconstruction of a pseudoaneurysm caused by stab wound 12 years ago.
J Zhejiang Univ Sci B
PUBLISHED: 05-13-2009
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Gastrointestinal bleeding due to aortoenteric fistula is extremely rare. Aortoenteric fistula is difficult to be diagnosed timely and entails a significant morbidity and mortality. Herein, we present an uncommon case of gastrointestinal bleeding caused by aortoduodenal fistula, which was a complication of a successful aortic reconstruction 4 months ago for an aortic pseudoaneurysm resulted from a stab wound 12 years ago. An urgent laparotomy confirmed an aortoduodenal fistula and repaired the defects in aorta and duodenum, but a prolonged shock led to the patients death. In summary, early diagnosis and surgical intervention for aortoenteric fistula are vital for survival.
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Electrophysiological correlates for response inhibition in intellectually gifted children: a Go/NoGo study.
Neurosci. Lett.
PUBLISHED: 03-02-2009
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Superior response inhibition is an essential component of the advanced cognitive abilities of gifted children. This study investigated response inhibition in intellectually gifted children by recording event-related brain potentials (ERPs) during a Go/NoGo task. Fifteen intellectually gifted children and 15 intellectually average children participated. Our present findings showed that intellectually gifted children had shorter Go-P3 latency, indicating faster processing of Go stimuli, a finding consistent with previous studies. We focused on the two inhibition-related components, NoGo-N2 and NoGo-P3. The results showed that NoGo-P3 latency was shorter for intellectually gifted children compared to their average peers. N2 latency did not indicate the intelligence difference. These results suggested that intellectually gifted children showed faster inhibition when dealing with NoGo stimuli, and this superiority came from the later stages of inhibition, i.e., response evaluation or the success of inhibiting a response, as indexed by the shorter P3 latency.
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Drug-resistant gene based genotyping for Acinetobacter baumannii in tracing epidemiological events and for clinical treatment within nosocomial settings.
Chin. Med. J.
PUBLISHED: 02-25-2009
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Acinetobacter baumannii has emerged as an important pathogen related to serious infections and nosocomial outbreaks around the world. However, of the frequently used methods, pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) in Acinetobacter baumannii genotyping lack the direct molecular proof of drug resistance. This study was conducted to establish a typing method based on drug resistant gene identification in contrast to traditional PFGE and AFLP in the period of nosocomial epidemic or outbreak.
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Mycoplasma lipoproteins and Toll-like receptors.
J Zhejiang Univ Sci B
PUBLISHED: 02-07-2009
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Mycoplasmas, the smallest free-living, self-replicating bacteria with diameters of 200 to 800 nm, have been reported to be associated with human diseases. It is well known that the mycoplasma lipoprotein/peptide is able to modulate the host immune system, whose N-terminal structure is an important factor in inducing immunity and distinguishing Toll-like receptors (TLRs). However, there is still no clear elucidation about the pathogenic mechanism of mycoplasma lipoprotein/peptide and the signaling pathway. Some researchers have focused on understanding the structures of these proteins and the relationships between their structure and biological function. This review provides an update on the research in this field.
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Splenomegaly as the first manifestation of pancreatic adenosquamous carcinoma: a case report.
Turk J Gastroenterol
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Adenosquamous carcinoma of the pancreas directly invading surrounding organs is rare. Here, we describe a case of pancreatic adenosquamous carcinoma directly invading the spleen and colon in a 44-year-old man. Imaging examinations revealed a huge heterogeneous mass in the enlarged spleen and a colonic lump, but showed no obvious space-occupying lesion in the pancreas. An exploratory laparotomy revealed that the enlarged spleen, pancreatic tail, and splenic flexure of the colon were firmly attached to one another. Splenectomy combined with resection of the distal pancreas and splenic flexure of the colon was performed. In the distal pancreatectomy specimen, a 0.8-cm solid mass was present in the tail. The tumor contained definite components of both adenocarcinoma and squamous cell carcinoma. The patient died of cachexia five months after admission. This case is unique in that the metastatic tumor involving the spleen was considerably larger than the primary tumor itself.
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Development and evaluation of a MAb-based ELISA for detection of Chlamydophila pneumoniae infection with variable domain 2 and 3 of the major outer membrane protein.
Biomed. Environ. Sci.
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This paper aims to develop a monoclonal antibodies (MAbs)- based ELISA for detecting Chlamydophila pneumoniae (C. pneumoniae) antigens in humans with the variable domains (VD) 2 and 3 of the major outer membrane protein (MOMPVD2-VD3) and to assess its sensitivity and specificity by comparing with a widely used MAb that is able to recognize the elementary bodies of C. pneumoniae.
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Laparoscopic resection of synchronous intraductal papillary mucinous neoplasms: a case report.
World J. Gastroenterol.
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We describe herein a 68-year-old woman who was diagnosed with a quite rare entity of intraductal papillary mucinous neoplasms (IPMNs) occurring simultaneously in the left lateral lobe of liver and the tail of pancreas. Abdominal computed tomography and magnetic resonance cholangiopancreatography showed a cystic dilatation of the pancreatic duct in the pancreatic tail, which suggested an IPMN, and multiple intrahepatic duct stones in the left lateral lobe. The patient underwent a laparoscopic left lateral hepatolobectomy and spleen-preserving distal pancreatectomy. Intra-operative finding of massive mucin in the dilated bile duct implied an intraductal mucinous tumor in the liver. The diagnosis of synchronous IPMNs in the liver and pancreas was confirmed by pathological examination. The patient was followed up for 6 mo without signs of recurrence. Although several cases of IPMN of liver without any pancreatic association have been reported, the simultaneous occurrence of IPMNs in the liver and pancreas is very rare. To the best of our knowledge, it is the first reported case treated by laparoscopic resection.
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[Laparoscopic distal pancreatectomy: the experience of 68 cases in a single centre].
Zhonghua Wai Ke Za Zhi
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To evaluate the feasibility and efficacy of laparoscopic distal pancreatectomy.
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Intelligent bioengineering in vitiligo treatment: transdermal protein transduction of melanocyte-lineage-specific genes.
Med. Hypotheses
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Vitiligo is a common, incurable skin disease with a prevalence of about 1%. Although many vitiligo therapies are available in clinics, there is almost no one method that causes significant improvement in all vitiligo patients. Some have hypothesized that melanocyte dysfunction or deficiency underlies the loss of skin pigmentation observed in vitiligo. The autoimmune-mediated apoptosis of melanocytes might be an important part of the etiology of vitiligo, which prevents the formation of melanocytes in the skin. Here we propose a novel hypothesis for vitiligo treatment using in situ melanocyte regeneration induced by melanocyte-lineage-specific genes (MLSGs). This may serve as an intelligent bioengineering prototype. The hypothesis is based on the fact that MLSGs regulate melanocyte differentiation through epigenetic reprogramming, which includes microphthalmia-associated transcription factor (MITF), paired box 3 (PAX3), and Notch signaling. MITF directs the terminal differentiation of melanocytes, and PAX3 helps to establish the properties of the melanocyte stem cells. Notch signaling promotes adult stem cell proliferation and self-renewal. This process could be mimicked by Notch intracellular domain (NICD). MLSGs could also stimulate anti-apoptotic gene expression. Recent improvements in relevant biotechniques allow the transdermal delivery of MLSG proteins into the patient, where they enter cells through protein transduction. This process may promote melanocyte regeneration in situ with little impact on the hair follicular cycle or on carcinogenesis. This simple and efficient treatment may have significant impact on the treatment of vitiligo patients.
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[Necessity of routine nasogastric decompression after gastrectomy for gastric cancer: a meta-analysis].
Zhonghua Yi Xue Za Zhi
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To evaluate the necessity of routine nasogastric decompression after radical gastrectomy for gastric cancer with systematic review and Meta-analysis.
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Laparoscopic distal pancreatectomy is as safe and feasible as open procedure: a meta-analysis.
World J. Gastroenterol.
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To evaluate the feasibility and safety of laparoscopic distal pancreatectomy (LDP) compared with open distal pancreatectomy (ODP).
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Successful en bloc resection of papillary neuroendocrine tumors by duodenoscope using endoscopic submucosal dissection method.
Clin Res Hepatol Gastroenterol
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Neuroendocrine tumors (NETs) of the main duodenal papilla are rare. Surgery such as pancreaticoduodenectomy is traditionally recommended as the standard treatment for papillary neuroendocrine tumors. Endoscopic resection is increasingly used for treating these lesions. However, resection of papillary NETs using endoscopic submucosal dissection (ESD) technique was scarcely reported. In this paper, we report a patient with neuroendocrine tumor of the main duodenal papilla originating from the muscularis propria on EUS underwent successful endoscopic submucosal dissection using a duodenoscope. With 6-month followed up, no stenosis of bile and pancreatic ducts or relapse of tumor occurred.
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[Construction of miRNA expression vector dual-targeting on HIF-1?/survivin genes and its effects on proliferation of pancreatic cancer Panc-1 cell line].
Zhejiang Da Xue Xue Bao Yi Xue Ban
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To design and construct miRNA expression vector dual-targeting on HIF-1? and survivin genes and to investigate its effects on proliferation of human pancreatic cancer cells.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.