JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Circulating betatrophin levels are increased in patients with type 2 diabetes and associated with insulin resistance.
J. Clin. Endocrinol. Metab.
PUBLISHED: 10-11-2014
Show Abstract
Hide Abstract
Context: Betatrophin has recently attracted increasing interests as a potential ?-cell regenerative therapy in diabetes. However, differences in betatrophin profiles in patients with type 2 diabetes mellitus (T2DM) remain unclear. Objective: To examine circulating betatrophin levels in subjects with different glucose tolerance status and its correlation with insulin resistance. Design, Setting and Participants: Serum betatrophin levels were measured using ELISA in age-, sex-, BMI- and blood lipids- matched subjects with normal glucose tolerance (NGT) (n=137), isolated impaired fasting glucose (IFG) (n=69), isolated impaired glucose tolerance (IGT) (n=120) and newly diagnosed T2DM (n=112) from the Risk Evaluation of cAncers in Chinese diabeTic Individuals: a lONgitudinal (REACTION) study. Results: Serum betatrophin levels were elevated in patients with T2DM compared to subjects with NGT, isolated IFG or isolated IGT (798.6 ± 42.5 vs. 692.7 ± 29.0, P < 0.05; vs. 682.7 ± 43.0, P < 0.05; vs. 646.8 ± 34.3, P < 0.01). Betatrophin levels positively correlated with index of homeostasis model assessment of insulin resistance (HOMA-IR) (partial r = 0.11); inversely correlated with quantitative insulin sensitivity check index (QUICKI) (partial r = -0.11), the Gutt insulin sensitivity index (ISIG) (partial r = -0.12) and the Matsuda insulin sensitivity index (ISIM) (partial r = -0.11) after controlling for age, sex, BMI and blood lipid in all participants (all P values < 0.05). Conclusion: Circulating betatrophin levels are increased in patients with T2DM and associated with indexes of insulin resistance.
Related JoVE Video
Associations study of vitamin D receptor gene polymorphisms with diabetic microvascular complications: a meta-analysis.
Gene
PUBLISHED: 03-05-2014
Show Abstract
Hide Abstract
Emerging evidence from preclinical and clinical studies has shown that vitamin D plays an important role in the pathogenesis of diabetic microvascular complications (DMI). Several potentially functional polymorphisms (ApaI, BsmI, FokI and TaqI) of vitamin D receptor (VDR) gene have been implicated in DMI risk, but individually published studies showed inconclusive results. The aim of this study was to quantitatively summarize the association between VDR polymorphisms and DMI risk.
Related JoVE Video
Leucocyte telomere shortening in relation to newly diagnosed type 2 diabetic patients with depression.
Oxid Med Cell Longev
PUBLISHED: 02-14-2014
Show Abstract
Hide Abstract
The goal of this study is to investigate the association between oxidative stress and telomere length shortening in the comorbid depression and diabetes. Therefore, 71 patients with newly diagnosed type 2 diabetes (T2D) and 52 subjects with normal glycemic level (control, Ctrl) were enrolled. Depressive status was identified with the Depression Subscale of Hospital Anxiety and Depression Scale (HADS-D). Leukocyte telomere length ratio (T/S ratio) was determined with quantitative PCR. Oxidative stress status was evaluated with 8-hydroxy-desoxyguanosine (8-OHdG) assay kit. Some other biochemical blood testing was also performed. The data showed that T2D patients had higher proportion of depression evaluated by the HADS-D (x(2) = 4.196, P = 0.041). T/S ratio was significantly negatively correlated with 8-OHdG, HADS-D, age, HbA1c, FPG, and HOMA-IR. In addition, HADS-D was significantly positively correlated with HbA1c, FPG, HOMA-IR, and 8-OHdG. Both HADS-D and 8-OHdG were the major independent predictors for T/S ratio. This study indicates that oxidative stress contributes to both telomere length shortening and depression development in newly diagnosed type 2 diabetic patients, while in depression status, some other mechanisms besides oxidative stress may also affect the telomere length.
Related JoVE Video
Short-term effects of continuous subcutaneous insulin infusion therapy in perioperative patients with diabetes mellitus.
Diabetes Technol. Ther.
PUBLISHED: 08-24-2013
Show Abstract
Hide Abstract
Abstract Background: Hyperglycemia is common and hard to control in surgical patients with diabetes. We retrospectively investigated short-term effects of continuous subcutaneous insulin infusion (CSII) in perioperative patients with diabetes. Patients and Methods: Perioperative patients with diabetes discharged between January 1, 2006 and January 1, 2012 were included. Glucose control and postoperative outcomes were compared between the patients using CSII or non-CSII insulin therapy. Results: We identified 108 pairs of patients matched by propensity and surgical category who were using CSII therapy (CSII group) or non-CSII insulin therapy (control group). The CSII group had significantly lower fasting glucose levels (on the first postoperative day, 9.06±3.09?mmol/L vs. 11.05±4.19?mmol/L; P=0.003) and lower mean glucose levels (on the operation day, 9.93±2.65?mmol/L vs. 12.05±3.86?mmol/L; P=0.001). The CSII group also had a lower incidence of fever (on the first postoperative day, 30.4% vs. 53.2%; P=0.005). Furthermore, patients in the CSII group experienced significantly shorter postoperative intervals for suture removal (P=0.02) and hospital discharge (P=0.03). No significant difference in the total medical expenditure was observed between the two groups (P=0.47). We also made a comparison between the 30 pairs of patients who were using CSII or multiple daily insulin injection therapy but observed no significant difference between these two therapies in glucose control or postoperative outcomes. Conclusions: Compared with non-CSII insulin therapy, even short-term implementation of CSII can improve the postoperative control of glucose, reduce the incidence of postoperative fever, and shorten the time for suture removal and discharge in surgical patients with diabetes.
Related JoVE Video
SREBP-1c, Pdx-1, and GLP-1R involved in palmitate-EPA regulated glucose-stimulated insulin secretion in INS-1 cells.
J. Cell. Biochem.
PUBLISHED: 07-01-2010
Show Abstract
Hide Abstract
Impairment of glucose-stimulated insulin secretion (GSIS) caused by glucolipotoxicity is an essential feature in type 2 diabetes mellitus (T2DM). Palmitate and eicosapentaenoate (EPA), because of their lipotoxicity and protection effect, were found to impair or restore the GSIS in beta cells. Furthermore, palmitate was found to up-regulate the expression level of sterol regulatory element-binding protein (SREBP)-1c and down-regulate the levels of pancreatic and duodenal homeobox (Pdx)-1 and glucagon-like peptide (GLP)-1 receptor (GLP-1R) in INS-1 cells. To investigate the underlying mechanism, the lentiviral system was used to knock-down or over-express SREBP-1c and Pdx-1, respectively. It was found that palmitate failed to suppress the expression of Pdx-1 and GLP-1R in SREBP-1c-deficient INS-1 cells. Moreover, down-regulation of Pdx-1 could cause the low expression of GLP-1R with/without palmitate treatment. Additionally, either SREBP-1c down-regulation or Pdx-1 over-expression could partially alleviate palmitate-induced GSIS impairment. These results suggested that sequent SREBP-1c-Pdx-1-GLP-1R signal pathway was involved in the palmitate-caused GSIS impairment in beta cells.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.