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Find video protocols related to scientific articles indexed in Pubmed.
Method to remove the effect of ambient temperature on radiometric calibration.
Appl Opt
PUBLISHED: 10-17-2014
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High precision radiometric calibration is essential for infrared imaging systems, especially in scientific applications where an accurate quantitative analysis is required. Nevertheless, calibration and radiometry are usually not simultaneously performed. Hence the discrepancy of ambient temperature between calibration and actual measurement can generate significant measurement errors unless the calibration results have been properly corrected. To overcome the restriction, we studied the effect of ambient temperature on radiometric calibration, then derived the relationship between calibration results and ambient temperature considering the integration time. A novel method compensating for the impact of ambient temperature on the calibration of a cooled infrared system is proposed. Several experiments are performed, and the results indicate that the proposed method can not only ensure the accuracy of calibration but achieve calibration results under any ambient temperature and arbitrary integration time.
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From Bad to Worse: Anemia on Admission and Hospital-Acquired Anemia.
J Patient Saf
PUBLISHED: 10-08-2014
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Anemia at hospitalization is often treated as an accompaniment to an underlying illness, without active investigation, despite its association with morbidity. Development of hospital-acquired anemia (HAA) has also been associated with increased risk for poor outcomes. Together, they may further heighten morbidity risk from bad to worse.
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Magnitude of Anemia at Discharge Increases 30-Day Hospital Readmissions.
J Patient Saf
PUBLISHED: 09-02-2014
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Anemia during hospitalization is associated with poor health outcomes. Does anemia at discharge place patients at risk for hospital readmission within 30 days of discharge? Our objectives were to examine the prevalence and magnitude of anemia at hospital discharge and determine whether anemia at discharge was associated with 30-day readmissions among a cohort of hospitalizations in a single health care system.
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Diagnostic utility of cardiac biomarkers in discriminating Takotsubo cardiomyopathy from acute myocardial infarction.
J. Card. Fail.
PUBLISHED: 08-05-2014
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Background: Takotsubo cardiomyopathy (TC) mimics acute myocardial infarction (AMI). We postulated that ventricular dysfunction in TC in the absence of significant myocardial necrosis would produce higher B-type natriuretic peptide (BNP)/troponin T (TnT) and BNP/creatine kinase MB fraction (CKMB) ratios than in AMI.Methods and Results: We studied 58 consecutive TC (age 65.8 +/- 12.9) and 97 AMI patients (age 59.8 +/-13.4). The ratios of BNP/TnT and BNP/CKMB were calculated with the use of first simultaneously drawn laboratory values. Receiver operating characteristic curves were used to distinguish TC from AMI with 95% specificity based on cardiac biomarker ratios. Median BNP/TnT and BNP/CKMB ratios were, respectively,1,292 [interquartile range 443.4-2,657.9] and 28.44 [13.7-94.8] in the TC group and 226.9[69.91-426.32] and 3.63 [1.07-10.02] in the AMI group (P <.001). TC can be distinguished from AMI with 95% specificity with the use of BNP/TnT ratio ?1,272 (sensitivity 52%) and BNP/CKMB ratio ?29.9 (sensitivity 50%).Conclusions: The value of BNP is significantly higher in TC than in AMI. Early BNP/TnT and BNP/CKMB ratios help to differentiate TC from AMI with greater accuracy than BNP alone.
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Proteomic View of Basement Membranes from Human Retinal Blood Vessels, Inner Limiting Membranes, and Lens Capsules.
J. Proteome Res.
PUBLISHED: 07-04-2014
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Basement membranes (BMs) are extracellular matrix sheets comprising the laminins, type-IV collagens, nidogens, and the heparan sulfate proteoglycans, perlecan, collagen XVIII, and agrin. In intact BMs, BM proteins are physiologically insoluble and partially resistant to proteolytic digestion, making BMs a challenge to study. Here three types of BMs from adult human eyes, the inner limiting membrane (ILM), the retinal vascular BMs, and the lens capsule, were isolated for analysis by 1D-SDS-PAGE and LC-MS/MS. Peptide and protein identifications were done using MaxQuant. 1129 proteins were identified with a 1% false discovery rate. Data showed that BMs are composed of multiple laminins, collagen IVs, nidogens, and proteoglycans. The dominant laminin family member in all BMs was laminin ?5?2?1. The dominant collagen IV trimer in lens capsule (LC) and blood vessel (BV) BMs had a chain composition of ?1(IV)2, ?2 (IV), whereas the dominant collagen IV in the ILM had the ?3(IV), ?4(IV), ?5(IV) chain composition. The data also showed that the ratio of laminin and collagen IVs varied among different BM types: the ratio of collagen IV to the other BM proteins is highest in LC, followed by BV and lowest for the ILM. The data have been deposited to the ProteomeXchange with identifier PXD001025.
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Single-center study of interrater agreement in the identification of central line-associated bloodstream infection.
Am J Infect Control
PUBLISHED: 02-26-2014
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Interrater reliability of central line-associated bloodstream infection (CLABSI) determination has not been well studied. The present study evaluated interrater reliability between infection preventionists (IPs) for CLABSI- and other bloodstream infection (BSI)-related factors and examined whether any nurse characteristics are associated with interrater reliability.
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Spectral CT Demonstration of the Superior Mesenteric Artery: Comparison of Monochromatic and Polychromatic Imaging.
Acad Radiol
PUBLISHED: 09-05-2013
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To investigate the performance of spectral computed tomography (CT) in depiction of the superior mesenteric artery (SMA) compared to conventional polychromatic CT.
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Diagnostic utility of cardiac biomarkers in discriminating Takotsubo Cardiomyopathy from Acute Myocardial Infarction.
J. Card. Fail.
PUBLISHED: 08-10-2013
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Takotsubo cardiomyopathy (TC) mimics acute myocardial infarction (AMI). We postulated that ventricular dysfunction in TC in the absence of significant myocardial necrosis would produce higher brain natriuretic peptide (BNP)/troponin T (TnT) and BNP/creatine kinase-MB (CKMB) ratios than in AMI.
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Endovascular versus open elephant trunk completion for extensive aortic disease.
J. Thorac. Cardiovasc. Surg.
PUBLISHED: 07-15-2013
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To compare the outcomes between patients undergoing endovascular (EEC) or open (OEC) approaches to second-stage elephant trunk completion (EC).
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Vaccination inhibits TLR2 transcription via suppression of GR nuclear translocation and binding to TLR2 promoter in porcine lung infected with Mycoplasma hyopneumoniae.
Vet. Microbiol.
PUBLISHED: 05-21-2013
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Toll-like receptors (TLRs) and glucocorticoid receptor (GR) act respectively as effectors of innate immune and stress responses. The crosstalk between them is critical for the maintenance of homeostasis during the immune response. Vaccination is known to boost adaptive immunity, yet it remains elusive whether vaccination may affect GR/TLR interactions following infection. Duroc×Meishan crossbred piglets were allocated to three groups. The control group (CC) received neither vaccination nor infection; the non-vaccinated infection group (NI) was artificially infected intratracheally with Mycoplasma hyopneumoniae (M. hyopneumoniae); while the vaccinated, infected group (VI) was vaccinated intramuscularly with inactivated M. hyopneumoniae one month before infection. The clinical signs and macroscopic lung lesions were significantly reduced by vaccination. However, vaccination did not affect the concentration of M. hyopneumoniae DNA in the lung. Serum cortisol was significantly decreased in both NI and VI pigs (P<0.01), but only VI pigs demonstrated significantly diminished nuclear GR content. TLRs 1-10 were all expressed in lung, among which TLR2 was the most abundant and was significantly up-regulated (P<0.05) in NI pigs, but not in VI pigs. Accordingly, GR binding to the GR response element on TLR2 promoter was significantly increased (P<0.05) in NI pigs, but not in VI pigs. These results suggest that the inhibition of GR nuclear translocation and binding to the TLR2 promoter, which results in diminished TLR2 expression, is associated with the protective effect of vaccination on M. hyopneumoniae-induced lung lesions in the pig.
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CCAAT/enhancer-binding protein ? is involved in the breed-dependent transcriptional regulation of 3?-hydroxysteroid dehydrogenase/?(5)-?(4)-isomerase in adrenal gland of preweaning piglets.
J. Steroid Biochem. Mol. Biol.
PUBLISHED: 04-29-2013
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The enzyme 3?-hydroxysteroid dehydrogenase/?(5)-?(4)-isomerase (3?-HSD) catalyzes the biosynthesis of all steroid hormones. The molecular mechanisms regulating porcine adrenal 3?-HSD expression in different breeds are still poorly understood. In this study, we aimed to compare the expression of 3?-HSD between preweaning purebred Large White (LW) and Erhualian (EHL) piglets and to explore the potential factors regulating 3?-HSD transcription. EHL had significantly higher serum levels of cortisol (P<0.01) and testosterone (P<0.01), which were associated with significantly higher expression of 3?-HSD mRNA (P<0.01) and protein (P<0.05) in the adrenal gland, compared with LW piglets. The 5 flanking region of the porcine 3?-HSD gene showed significant sequence variations between breeds, and the sequence of EHL demonstrated an elevated promoter activity (P<0.05) in luciferase reporter gene assay. Higher adrenal expression of 3?-HSD in EHL was accompanied with higher CCAAT/enhancer binding protein ? (C/EBP?) expression (P<0.05), enriched histone H3 acetylation (P<0.05) and C/EBP? binding to 3?-HSD promoter (P<0.05). In addition, higher androgen receptor (AR) (P=0.06) and lower glucocorticoid receptor (GR) (P<0.05) were detected in EHL. Co-immunoprecipitation analysis revealed interactions of C/EBP? with both AR and GR. These results indicate that the C/EBP? binding to 3?-HSD promoter is responsible, at least in part, for the breed-dependent 3?-HSD expression in adrenal gland of piglets. The sequence variations of 3?-HSD promoter and the interactions of AR and/or GR with C/EBP? may also participate in the regulation.
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Early removal of urinary catheters in patients with thoracic epidural catheters.
J Nurs Care Qual
PUBLISHED: 04-26-2013
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The purpose of this study was to determine whether early removal of urinary catheters in patients with thoracic epidurals resulted in urinary retention (>500 mL by bladder scanner). Patients were given up to 8 hours to void before further intervention. Of 61 patients, only 4 (6.6%) required urinary catheter reinsertion due to urinary retention. Early removal of urinary catheters after thoracic surgery in patients with thoracic epidurals was safe, with minimal urinary retention.
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Hospital-acquired anemia: Prevalence, outcomes, and healthcare implications.
J Hosp Med
PUBLISHED: 03-11-2013
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Evidence suggests that patients with normal hemoglobin (Hgb) levels on hospital admission who subsequently develop hospital-acquired anemia (HAA) may be at risk for adverse outcomes. Our objectives were to (1) determine the prevalence of HAA and (2) examine whether HAA is associated with increased mortality, length of stay (LOS), and total hospital charges.
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A direct comparison of early and late outcomes with three approaches to carotid revascularization and open heart surgery.
J. Am. Coll. Cardiol.
PUBLISHED: 02-27-2013
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The aim of this study was a comparison of risk-adjusted outcomes of 3 approaches to carotid revascularization in the open heart surgery (OHS) population.
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Statin therapy is associated with fewer infections after cardiac operations.
Ann. Thorac. Surg.
PUBLISHED: 02-04-2013
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Statins interact with multiple pathways involved in infection. Therefore, we examined the association between preoperative statin therapy and infections after cardiac operations and assessed whether statin therapy was associated with lower infection-related mortality.
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Validation of whole slide imaging for primary diagnosis in surgical pathology.
Arch. Pathol. Lab. Med.
PUBLISHED: 01-16-2013
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High-resolution scanning technology provides an opportunity for pathologists to make diagnoses directly from whole slide images (WSIs), but few studies have attempted to validate the diagnoses so obtained.
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Enhanced oxygen reduction reactions in fuel cells on H-decorated and B-substituted graphene.
Chemphyschem
PUBLISHED: 01-09-2013
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In the light of recent experimental research on the oxygen reduction reaction (ORR) with carbon materials doped with foreign atoms, we study the performance of graphene with different defects on this catalytic reaction. In addition to the reported N-graphene, it is found that H-decorated and B-substituted graphene can also spontaneously promote this chemical reaction. The local high spin density plays the key role, facilitating the adsorption of oxygen and OOH, which is the start of ORR. The source of the high spin density for all of the doped graphene is attributed to unpaired single ? electrons. Meanwhile, the newly formed C-H covalent bond introduces a higher barrier to the p electron flow, leading to more localized and higher spin density for H-decorated graphene. At the same time, larger structural distortion should be avoided, which could impair the induced spin density, such as for P-substituted graphene.
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Predictors for electronic survey completion in healthcare research.
Comput Inform Nurs
PUBLISHED: 09-22-2011
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Few studies have examined patients preferences for and predictors of completing health surveys by paper versus Internet. The purpose of this study was to examine if participants of registry research preferred to complete health surveys by the Internet or paper, and if demographics and previous computer experiences were associated with health survey completion method preference. Using a descriptive design and convenience sample, participants of colorectal surgery registries completed an 18-item survey about Internet use and personal characteristics. Multiple linear regressions were used to determine predictors of total Internet use and access and survey preference. In 526 participants, preference for Internet-based health survey completion was associated with younger age, higher education, computer ownership, and using e-health medical records (all P ? .01). Those who previously completed Internet-based health surveys were more often married or divorced and computer owners and had electronic access to health records (all P ? .001). After multivariable regression, the Internet use/access sum score was associated with computer ownership, using a secure Web-based system and preference for completing electronic health surveys (all P < .001). In conclusion, after controlling for demographics, computer ownership, comfort in using Web-based systems including surveys, and access to computerized health records predicted preference for completing research-based health surveys by the Internet.
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The frequency of low muscle mass and its overlap with low bone mineral density and lipodystrophy in individuals with HIV--a pilot study using DXA total body composition analysis.
J Clin Densitom
PUBLISHED: 07-25-2011
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As a result of the advances in antiretroviral therapy, the life span of human immunodeficiency virus (HIV)-infected patients has increased dramatically. Attendant to these effects are signs of premature aging with notable changes in the musculoskeletal system. Although changes in bone and fat distribution have been studied extensively, far less is known about changes in muscle. This study examined the extent of low muscle mass (LMM) and its relationship with low bone mineral density (BMD) and lipodystrophy (LD) in HIV-positive males. As such, HIV-positive males on therapy or treatment naive underwent dual-energy X-ray absorptiometry total body composition measurements. Appendicular lean mass/(height)2 and lowest 20% of residuals from regression analysis were used to define LMM. BMD criteria defined osteopenia/osteoporosis, and the percent central fat/percent lower extremity ratio defined LD. Several potential risk factors were assessed through chart review. Sixty-six males (57 with treatment and 9 treatment naive) volunteered. Treated individuals were older than naive (44 vs 34 yr) and had HIV longer (108 vs 14 mo). When definitions for sarcopenia (SP) in elderly individuals were applied, the prevalence of LMM was 21.9% and 18.8% depending on the definition used. Low BMD was present in 68.2% of participants. LD with a cutoff of 1.5 and 1.961 was present in 54.7% and 42.2% of participants, respectively. LMM and LD were negatively associated. In conclusion, this study shows that LMM is common in males with HIV and that SP affecting muscle function could be present in a substantial number of individuals. Future research needs to examine what impact decreased muscle mass and function has on morbidity, physical function, and quality of life in individuals with HIV.
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Complexity of care is associated with distressing environmental factors.
Clin Nurse Spec
PUBLISHED: 06-10-2011
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The purpose of this study was to describe work and cognitive complexity patterns and assess predictors of increased complexity of care.
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Peak circulatory power as an indicator of clinical status in children after Fontan procedure.
Pediatr Cardiol
PUBLISHED: 05-26-2010
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Peak circulatory power (CircP), a product of peak exercise oxygen uptake (VO(2)) and peak mean or systolic arterial blood pressure, has proved to be a strong predictor of poor outcome in adults with congenital heart disease. This study sought to compare CircP with other cardiopulmonary exercise (CPX) test variables and to assess whether CircP is superior in categorizing patients into well-functioning vs. poorly functioning at-risk groups in the pediatric population after a Fontan procedure. The CPX test reports of 50 patients were retrospectively reviewed after the Fontan procedure. The patients were divided into two groups. The well-functioning group included patients in New York Heart Association (NYHA) classes 1 and 2 (n = 36). The poorly functioning at-risk group included patients in NYHA classes 3 and 4 and those with significant indicators or outcomes of a poor prognosis (n = 14). The patients in the well-functioning group had significantly higher CircP values based on mean blood pressure (MBP) (P < 0.001), higher CircP values based on systolic blood pressure (SBP) (P < 0.001), and higher peak VO(2) (P = 0.004) than those in the poorly functioning at-risk group. At a cutoff value less than 2100.4 mmHg/mlO(2)/kg/min, CircP MBP had a sensitivity of 85% in categorizing children to the poorly functioning at-risk group. CircP correlated well with the clinical status of our patients. CircP and peak VO(2) did not differ significantly in ability to identify poorly functioning patients. Further prospective analysis is needed to assess whether CircP can serve as a prognostic marker for the pediatric population after Fontan procedure.
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Preoperative statin therapy is not associated with a reduced incidence of postoperative acute kidney injury after cardiac surgery.
Anesth. Analg.
PUBLISHED: 04-07-2010
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Our objective was to examine the association between preoperative statin therapy and the prevalence of postoperative acute kidney injury (AKI) in patients undergoing cardiac surgery with the use of cardiopulmonary bypass.
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Ecdysteroid regulation of ovarian growth and oocyte maturation in the red flour beetle, Tribolium castaneum.
Insect Biochem. Mol. Biol.
PUBLISHED: 02-24-2010
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Previous studies from our laboratory showed the involvement of juvenile hormone (JH) and ecdysteroid signaling in the regulation of female reproduction in the red flour beetle, Tribolium castaneum. JH regulates vitellogenin (Vg) synthesis in the fat body but the role of ecdysteroid signaling is not known. Here, we report on ecdysteroid regulation of ovarian growth and oocyte maturation. Microarray analysis of RNA isolated from ovaries showed the up-regulation of several genes coding for proteins involved in ecdysteroid signaling on the 4th day after female adult eclosion. The functional analyses of genes coding for proteins involved in ecdysteroid and JH signaling pathways by RNA interference (RNAi) revealed that ecdysteroids but not JH regulate ovarian growth and primary oocyte maturation. Ultrastructural studies showed the temporal sequences of key events in oogenesis including the development of primary oocytes, the differentiation and development of follicle epithelial cells, and the formation of intercellular spaces to facilitate uptake of Vg protein. RNAi studies showed that ecdysone receptor (EcR) and ultraspiracle (USP) are required for the ovarian growth, primary oocyte maturation and the growth and migration of the follicle cells. These studies suggest important roles for ecdysteroids in the regulation of oocyte maturation in the beetle ovaries.
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Juvenile hormone regulation of vitellogenin synthesis in the red flour beetle, Tribolium castaneum.
Insect Biochem. Mol. Biol.
PUBLISHED: 02-24-2010
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To elucidate the endocrine regulation of vitellogenin (Vg) synthesis in the red flour beetle, Tribolium castaneum, the titers of juvenile hormone (JH) and ecdysteroids in the whole body of female beetles were measured and compared with Vg mRNA levels. Juvenile hormone levels remained high while the ecdysteroid levels declined steadily during 1-5 days post adult emergence (PAE). The Vg mRNA levels began to increase by the end of 3rd day PAE and peaked by the 4th-5th day PAE. Gene expression profiling by microarray and quantitative real-time PCR analyses of RNA isolated from 1 to 5 days PAE beetles revealed that the genes coding for proteins involved in JH biosynthesis and action, but not those involved in 20-hydroxyecdysone (20E) biosynthesis and action had similar expression patterns as the genes coding for Vg. RNA interference (RNAi)-aided knock-down in the expression of these genes showed that both JH and 20E were required for Vg gene expression. However, Vg mRNA was induced by the application of JH III but not by the injection of 20E into the previtellogenic females. These data suggest that JH is required for Vg synthesis in the fat body and 20E influences Vg synthesis through its action on oocyte maturation.
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Effectiveness of implantable cardioverter-defibrillator lead coil treatments in facilitating ease of extraction.
Heart Rhythm
PUBLISHED: 02-10-2010
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Expanded polytetrafluoroethylene (ePTFE) coated and medical adhesive back-filled (MABF) implantable cardioverter-defibrillator (ICD) coils have been promoted to reduce the fibrous ingrowth into coils that can complicate lead extraction.
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Pediatric specialists beliefs about gastroesophageal reflux disease in premature infants.
Pediatrics
PUBLISHED: 12-14-2009
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Wide variation exists in the treatment of suspected gastroesophageal reflux disease (GERD) in premature infants; it is unknown to what degree diagnosis and treatment are affected by the treating physicians medical specialty or interpretation of the medical literature.
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Patterns of procedure-specific radiation exposure in the endovascular era: impetus for further innovation.
J. Vasc. Surg.
PUBLISHED: 02-11-2009
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Although patient preference and outcome data support continued development and use of minimally invasive endovascular therapies, only a few studies have documented radiation exposure to the patient. This report summarizes patient radiation exposure by endovascular procedure at Cleveland Clinic.
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Sleep apnea and epilepsy: whos at risk?
Epilepsy Behav
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Obstructive sleep apnea (OSA) is highly prevalent, affecting 25% of men and 10% of women. Treatment reduces seizures in some patients. Awareness of the comorbidity of sleep disturbances in epilepsy has been increasing. No study has explored OSA predictors in patients unselected for epilepsy severity and sleep disorder symptoms. We assessed cross-sectional OSA prevalence and predictors (apnea-hypopnea index [AHI] ?10) in 130 consecutive adults using structured interview, subjective assessments, and polysomnography. Obstructive sleep apnea prevalence was 30%, 16% having moderate-severe disease, rates that markedly exceed general population estimates. Obstructive sleep apnea predictors in multivariate modeling included age, dental problems, and standardized AED dose. Male gender, older age, higher BMI, hypertension, and dental problems were associated with higher AHI. Adults with epilepsy appear at increased risk for OSA, increasing with age and AED load, regardless of gender, BMI, and seizure frequency. These findings support the implementation of routine OSA screening in adult epilepsy clinics.
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The positive influence of boron-doped graphene for its supported Au clusters: enhancement of SERS and oxygen molecule adsorption.
Phys Chem Chem Phys
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The interactions between Au clusters and graphene sheets with and without doping have been studied systematically. It is found that B-doped graphene is a good support for Au clusters, not only because of the charge transfer from the graphene sheet to its supported Au clusters, but also due to its stronger interaction at the interface resulting from the doped B atom, which will facilitate more electrons being transferred to the upper surface of the Au cluster. This is good for surface-enhanced Raman scattering (SERS) and molecule oxygen adsorption, beneficial to the material preparation and apparatus design for high active SERS substrates and nano-catalysts.
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Characteristics of duplex sonographic parameters over time after successful carotid artery stenting.
J Ultrasound Med
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Carotid duplex sonography is the primary tool for surveillance after carotid artery stenting, but the course of sonographic velocities over time after successful stenting is unclear. The purpose of this study was to describe carotid duplex sonographic velocity parameters after successful carotid artery stenting and to determine the predictors of poststent sonographic velocities.
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Safety of "bridging" with eptifibatide for patients with coronary stents before cardiac and non-cardiac surgery.
Am. J. Cardiol.
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Patients with previously implanted coronary stents are at risk for stent thrombosis if dual-antiplatelet therapy is prematurely discontinued. Bridging with a glycoprotein IIb/IIIa inhibitor has been advocated as an alternative, with few supporting data. The aim of this study was to determine the safety of such a strategy by retrospectively analyzing bleeding in 100 consecutive patients with previously implanted coronary stents who were bridged to surgery with eptifibatide after discontinuing thienopyridine therapy. A propensity-matched control comparison was performed for a subgroup of 71 patients who underwent cardiovascular surgery. Blood transfusions were required in 65% in the bridged group versus 66% in the control group (p = 0.86). The mean numbers of units transfused were 4.84 ± 6.93 and 3.65 ± 7.46, respectively (p >0.25). Rates of return to the operating room for bleeding or tamponade were 10% and 2.9%, respectively (p = 0.085). Increased rates of transfusion were noted for patients who received concomitant aspirin and/or intravenous heparin infusion. In conclusion, there does not appear to be any increase in the need for blood transfusions or rate of return to the operating room for patients being bridged with eptifibatide when thienopyridines are discontinued in the perioperative period, but concomitant use of additional antiplatelet or anticoagulant agents may increase transfusions and delays to surgery. Clinicians who are considering this strategy must weigh the risks of stent thrombosis versus bleeding.
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Impact of isolation on hospital consumer assessment of healthcare providers and systems scores: is isolation isolating?
Infect Control Hosp Epidemiol
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The Hospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS) survey was used to measure the effect of isolation on patient satisfaction. Isolated patients reported lower scores for questions regarding physician communication and staff responsiveness. Overall scores for these domains were lower in isolated than in nonisolated patients.
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Diversity of a mesophilic lignocellulolytic microbial consortium which is useful for enhancement of biogas production.
Bioresour. Technol.
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A mesophilic lignocellulolytic microbial consortium BYND-5, established by successive subcultivation, was applied to enhance the biogas production. The degradation efficiency of BYND-5 for rice straw was more than 49.0 ± 1.8% after 7 days of cultivation at 30°C. Various organic compounds, including acetic acid, propionic acid, butyric acid and glycerin were detected during biodegradation. The diversity analysis of BYND-5 was conducted by ARDRA (Amplified ribosomal DNA restriction analysis) of the 16S rDNA clone library. Results indicated that bacterial groups represented in the clone library were the Firmicutes (5.96%), the Bacteroidetes (40.0%), Deferribacteres (8.94%), Proteobacteria (16.17%), Lentisphaerae (2.13%), Fibrobacteraceae (1.7%), and uncultured bacterium (25.1%). Additionally, the enhancement of biogas yield and methane content was directly related to the pretreatment with BYND-5. The microbial community identified herein is potential candidate consortium for the degradation of waste lignocellulose and enhancement of biogas production under the mesophilic temperature conditions.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.