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Bioengineering
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在小鼠心脏的动作电位和钙瞬变的光学测绘
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
Please note that all translations are automatically generated.
Click here for the English version.
在小鼠心脏的动作电位和钙瞬变的光学测绘
DOI:
10.3791/3275-v
•
08:13 min
•
September 13, 2011
•
Di Lang
,
Matthew Sulkin
,
Qing Lou
,
Igor R. Efimov
1
Department of Biomedical Engineering
,
Washington University in St. Louis
Chapters
00:05
Title
01:24
Preparing the Perfusion and Imaging Setup
03:23
Langendorff Perfusion of the Isolated Heart
05:25
Loading Voltage and Calcium Sensitive Dyes
06:38
Optical Imaging and Data Analysis
07:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文详细解剖程序,工具设置,并在光学测绘跨膜电位(VM)和细胞内钙瞬变(CAT),在隔离的Langendorff完整的实验条件下灌注小鼠心脏。
Tags
Optical Mapping
Action Potentials
Calcium Transients
Mouse Heart
Cardiovascular Studies
Fluorescence Imaging
Genetic Engineering
Physiological Phenotyping
Transmembrane Potential (Vm)
Excitation-contraction Coupling
Langendorff Perfused Mouse Hearts
Heart Failure
Arrhythmias
Metabolic Disease
Heart Diseases
Activation Visualization
Conduction Velocity
Action Potential Duration
Instrumentation Setup
Experimental Conditions
Spatio-temporal Resolution
CMOS Imaging Technology
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