Articles by Ana Farinho in JoVE
A Model of Free Tissue Transfer: The Rat Epigastric Free Flap Diogo Casal1,4, Diogo Pais1, Inês Iria3,4, Eduarda Mota-Silva5, Maria-Angélica Almeida2, Sara Alves6, Cláudia Pen6, Ana Farinho4, Luís Mascarenhas-Lemos1,6, José Ferreira-Silva6, Mário Ferraz-Oliveira6, Valentina Vassilenko5, Paula A. Videira3,4, João Gory O'Neill1,5 1Anatomy Department, NOVA Medical School, Universidade NOVA de Lisboa, 2Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central - Hospital de São José, 3UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, 4CEDOC, NOVA Medical School, Universidade NOVA de Lisboa, 5Physics Department, Faculty of Sciences and Technology, LIBPhys, 6Pathology Department, Centro Hospitalar de Lisboa Central – Hospital de São José This paper describes the steps required to raise a fasciocutaneous epigastric free flap and transfer it to the neck in the rat.
Other articles by Ana Farinho on PubMed
Denervation Impairs Regeneration of Amputated Zebrafish Fins BMC Developmental Biology. Dec, 2014 | Pubmed ID: 25551555 Zebrafish are able to regenerate many of its tissues and organs after damage. In amphibians this process is regulated by nerve fibres present at the site of injury, which have been proposed to release factors into the amputated limbs/fins, promoting and sustaining the proliferation of blastemal cells. Although some candidate factors have been proposed to mediate the nerve dependency of regeneration, the molecular mechanisms involved in this process remain unclear.
Control of Tissue Growth by Yap Relies on Cell Density and F-actin in Zebrafish Fin Regeneration Development (Cambridge, England). Aug, 2015 | Pubmed ID: 26209644 Caudal fin regeneration is characterized by a proliferation boost in the mesenchymal blastema that is controlled precisely in time and space. This allows a gradual and robust restoration of original fin size. However, how this is established and regulated is not well understood. Here, we report that Yap, the Hippo pathway effector, is a chief player in this process: functionally manipulating Yap during regeneration dramatically affects cell proliferation and expression of key signaling pathways, impacting regenerative growth. The intracellular location of Yap is tightly associated with different cell densities along the blastema proximal-distal axis, which correlate with alterations in cell morphology, cytoskeleton and cell-cell contacts in a gradient-like manner. Importantly, Yap inactivation occurs in high cell density areas, conditional to F-actin distribution and polymerization. We propose that Yap is essential for fin regeneration and that its function is dependent on mechanical tension, conferred by a balancing act of cell density and cytoskeleton activity.