In JoVE (1)
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Articles by Andoniya Petkova in JoVE
An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins Donatus Riemann1, Andoniya Petkova1, Thomas Dresbach1, Rebecca Wallrafen1 1Institute for Anatomy and Embryology, University Medical Centre Göttingen We describe an optical assay for synaptic vesicle (SV) recycling in cultured neurons. Combining this protocol with double transfection to express a presynaptic marker and protein of interest allows us to locate presynaptic sites, their synaptic vesicle recycling capacity, and determine the role of the protein of interest.