In JoVE (1)
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Articles by Andreas Hörnblad in JoVE
Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research Anna U. Eriksson*1, Christoffer Svensson*1, Andreas Hörnblad1, Abbas Cheddad1, Elena Kostromina1, Maria Eriksson1, Nils Norlin1, Antonello Pileggi2, James Sharpe3, Fredrik Georgsson4, Tomas Alanentalo1, Ulf Ahlgren1 1Umeå Centre for Molecular Medicine, Umeå University, 2Cell Transplant Center, Diabetes Research Institute, University of Miami,, 3EMBL-CRG Systems Biology Program, Centre for Genomic Regulation, Catalan Institute of Research and Advanced Studies, 4Dept. of Computing Science, Umeå University We describe the adaptation of optical projection tomography (OPT)1 to imaging in the near infrared spectrum, and the implementation of a number of computational tools. These protocols enable assessments of pancreatic β-cell mass (BCM) in larger specimens, increase the multichannel capacity of the technique and increase the quality of OPT data.