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Articles by Archana Kumar in JoVE
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Een praktische en nieuwe methode om Genomic DNA Uittreksel uit Bloedafname Kits voor Plasma Protein Preservation
Jon Waters1, Vishal Dhere1, Adam Benjamin1, Arvind Sekar1, Archana Kumar1, Sampath Prahalad2, David T. Okou1, Subra Kugathasan1
1Division of Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, Emory University School of Medicine and Children's Health Care of Atlanta, 2Division of Rheumatology, Department of Pediatrics, Emory University School of Medicine and Children's Health Care of Atlanta
We beschrijven een nieuwe werkwijze voor het isoleren van genomisch DNA uit volbloed voor plasma / serologie. Na het plasma, wordt de verdichte bloed meestal weggegooid. Onze nieuwe methode is een aanzienlijke verbetering ten opzichte van bestaande methoden en maakt DNA en plasma verkrijgbaar vanaf een enkele collectie, zonder daarvoor extra bloed.
Other articles by Archana Kumar on PubMed
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Blood Lead Levels in Children of Lucknow, India
Environmental Toxicology.
Feb, 2010 |
Pubmed ID: 19161238 After implementing measures to control lead pollution in many countries, blood lead levels (BLLs) in children have been declined. The phase-out of leaded petrol began in Lucknow, the capital of most populated state, Uttar Pradesh in India on April 1, 2000. We evaluated the mean BLL and associated risk factors for lead exposure in Lucknow children (3-12 years) after petrol lead phase-out activity. The mean BLL of the 200 children was 9.3 microg/dL (range: 1.0-27.9 microg/dL). Seventy-four children (37%) had BLL above the Centre for Disease Control and Prevention's (CDC) level of concern (10 microg/dL). When these data were compared with BLLs determined by the George Foundation among the children of Mumbai, Bangalore, Kolkata, Chennai, Hyderabad, and Delhi during the year 1997 when leaded petrol was in use, where 62%, 62%, 87%, 96%, 43%, and 95%, respectively, then exceeded the CDC intervention level. Further, BLL of present study was very close to Mumbai children (age < or =12 years) estimated following the introduction of unleaded petrol, and 33% children had BLL above the CDC concern level with an average 8.4 microg/dL. Low socioeconomic status, proximity of home to traffic density, and mother's illiteracy were the factors associated with elevated BLLs in Lucknow children (P < 0.05). Overall, results indicate a declining trend of BLL in Lucknow children when compared with those reported from other cities of India when leaded petrol was in practice. A national population-based study is recommended to determine the prevalence of elevated BLLs after the phase-out of leaded-petrol. In addition, future studies on other sources of lead exposure in Lucknow are warranted for public health purposes.
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